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| 1 | Cloning of 9-cis-epoxycarotenoid dioxygenase (NCED) gene encoding a key enzyme during abscisic acid (ABA) biosynthesis and ABA-regulated ethylene production in detached young persimmon calyx显示文摘Unlike the typical climacteric fruits, persimmons (Diospyros kaki Thunb.) produce higher levels of ethylene when they are detached from trees at a younger stage. In order to obtain detailed information on the role of abscisic acid (ABA) in ripening, we cloned the DKNCED1, DKACS2, and DKACO1 genes from the calyx. Water loss was first noted in the calyx lobe, and DKNCED1 was highly expressed 1 d after the fruits were detached, coinciding with an increase in the ABA content. Then, the DKACS2 and DKACO1 genes were expressed after some delay. In the calyx, the ABA peak was observed 2 d after the fruits were harvested, and this peak preceded the ethylene peak observed on day 3. The fruit firmness rapidly decreased on day 4, and the fruits softened completely 6 d after they were harvested. The increases in the expressions of ABA, ethylene, and the genes in the calyxes occurred earlier than the corresponding increases in the pulp, although the 3 increases occurred on different days. Exogenous ABA treatment increased ABA concentration, induced expression of both ACS and ACO, and promoted ethylene synthesis and young-fruit softening; by contrast, treatment with NDGA inhibited the gene expressions and ethylene synthesis and delayed young-fruit softening. These results indicate that ethylene biosynthesis in the detached young persimmon fruits is initially triggered by ABA, which is induced by water loss in the calyx, through the induction of DKACS2 and DKACO1 expressions. The ethylene produced in the calyx subsequently diffuses into the pulp tissue, where it induces autocatalytic ethylene biosynthesis, resulting in an abrupt increase in ethylene production. | LENG Ping ZHANG GuangLian LI XiangXin WANG LiangHe ZHENG ZhongMing | 2009 | Chinese Science Bulletin2009，54，16： | 13 |
| 2 | Inhibiting Hv1 channel in peripheral sensory neurons attenuates chronic inflammatory pain and opioid side effects显示文摘Both opioids and nonsteroidal anti-inflammatory drugs(NSAIDS)produce deleterious side effects and fail to provide sustained relief in patients with chronic inflammatory pain.Peripheral neuroinflammation(PN)is critical for initiation and development of inflammatory pain.A better understanding of molecular mechanisms underlying PN would facilitate the discovery of new analgesic targets and the development of new therapeutics.Emerging evidence suggests that peripheral sensory neurons are not only responders to painful stimuli,but are also actively engaged in inflammation and immunity,whereas the intrinsic regulatory mechanism is poorly understood.Here we report the expression of proton-selective ion channel Hv1 in peripheral sensory neurons in rodents and humans,which was previously shown as selectively expressed in microglia in mammalian central nervous system.Neuronal Hv1 was up-regulated by PN or depolarizing stimulation,which in turn aggravates inflammation and nociception.Inhibiting neuronal Hv1 genetically or by a newly discovered selective inhibitor YHV98-4 reduced intracellular alkalization and ROS production in inflammatory pain,mitigated the imbalance in downstream SHP-1-pAKT signaling,and also diminished pro-inflammatory chemokine release to alleviate nociception and morphine-induced hyperalgesia and tolerance.Thus,our data reveal neuronal Hv1 as a novel target in analgesia strategy and managing opioids-related side effects. | Qiansen Zhang Yimin Ren Yiqing Mo Peipei Guo Ping Liao Yuncheng Luo Jie Mu Zhuo Chen Yang Zhang Ya Li Linghui Yang Daqing Liao Jie Fu Juwen Shen Wei Huang Xuewen Xu Yanyan Guo Lianghe Mei Yunxia Zuo Jin Liu Huaiyu Yang Ruotian Jiang | 2022 | Cell Research2022，32，5： | 3 |
| 3 | Molecular basis for ligand activation of the human KCNQ2 channel显示文摘The voltage-gated potassium channel KCNQ2 is responsible for M-current in neurons and is an important drug target to treat epilepsy,pain and several other diseases related to neuronal hyper-excitability.A list of synthetic compounds have been developed to directly activate KCNQ2,yet our knowledge of their activation mechanism is limited,due to lack of high-resolution structures.Here,we report cryo-electron microscopy(cryo-EM)structures of the human KCNQ2 determined in apo state and in complex with two activators,ztz240 or retigabine,which activate KCNQ2 through different mechanisms.The activator-bound structures,along with electrophysiology analysis,reveal that ztz240 binds at the voltage-sensing domain and directly stabilizes it at the activated state,whereas retigabine binds at the pore domain and activates the channel by an allosteric modulation.By accurately defining ligand-binding sites,these KCNQ2 structures not only reveal different ligand recognition and activation mechanisms,but also provide a structural basis for drug optimization and design. | Xiaoxiao Li Qiansen Zhangc Peipei Guo Jie Fu Lianghe Mei Dashuai Lv Jiangqin Wang Dongwu Lai Sheng Ye Huaiyu Yang Jiangtao Guo | 2021 | Cell Research2021，31，1： | 3 |
| 4 | Transarterial infusion chemotherapy with FOLFOX for advanced hepatocellular carcinoma:a multi-center propensity score matched analysis of real-world practice显示文摘Background:To compare the treatment effectiveness and safety among transarterial infusion chemotherapy(TAI)with FOLFOX regimen,transarterial chemoembolization(TACE),and sorafenib in patients with BCLC stage C hepatocellular carcinoma(HCC).Methods:The data of consecutive patients with BCLC stage C HCC treated with TAI,TACE,or sorafenib from January 2015 to December 2018 at three centers were retrospectively analyzed.Propensity-score matched(PSM)analysis was pairwise performed to reduce selection bias.Treatment effectiveness and safety were evaluated and compared using the Kaplan-Meier method,log-rank test,Cox regression models,andχ2 test.Results:The median overall survival(OS)in the matched TAI cohort was significantly longer than the sorafenib cohort(19.6 vs.7.5 months,P=0.009),and the TACE cohort(estimated 27.8 vs.6.6 months,P<0.001).The difference in median progression-free survival(PFS)between the matched TAI and sorafenib cohorts was not significant(5.8 vs.2.3 months,P=0.219).The median PFS in the matched TAI cohort was significantly longer than the TACE cohort(6.5 vs.2.8 months,P<0.001).The objective response rate(ORR)in the matched TAI cohort was significantly higher than the sorafenib cohort(36.4%vs.0.0%,P<0.001)and the TACE cohort(48.7%vs.4.7%,P<0.001).The incidences of adverse events(AEs)were similar among these three cohorts.Conclusions:TAI with FOLFOX regimen was an effective and safe therapy that improved survival of patients with BCLC stage C HCC. | Shaohua Li Jie Mei Qiaoxuan Wang Feng Shi Hongyan Liu Ming Zhao Lianghe Lu Yihong Ling Zhixing Guo Yabing Guo Xiaoming Chen Ming Shi Wan Yee Lau Wei Wei Rongping Guo | 2021 | Hepatobiliary Surgery and Nutrition2021，10，5： | 2 |
| 5 | CrystallineplasGticityoncopper(001)(110)and(111)surfacesduringnanoindentation 显示文摘 | LiangH WooC H HuangH etal | 2004 | Computational Methodsin EngiGneeringScienceandMechanics2004，6，1： | 1 |
| 6 | Grayscale morphological filter for small target detection显示文摘 | Zhu Z F Li Z L LiangH C | 2000 | Proceedings of SPIE2000，4130，： | 1 |
| 7 | Ethanol extract of Fructus Ligustri Lucidi promotes osteogenesis of mesenchymal stem cells显示文摘 | GuoLi Xiao‐aiZhang Jin‐fangZhang Chu‐yanChan David Tai WaiYew Ming‐liangHe Marie Chia‐miLin Ping‐chungLeung Hsiang‐fuKung | 2010 | Phytother Res2010，，4： | 1 |
| 8 | SpectralCTimagingoflaryngealandhypo-pharyngealsquamouscellcarcinoma:Evaluationofimagequalityandstatusoflymphnodes显示文摘 | LiA LiangH LiW etal | 2013 | PLoSONE2013，8，83： | 1 |
| 9 | T helper cell effector fates-who,how and where显示文摘 | Reinhardtrl Kangs J Lianghe | 2006 | Curr opin Immunol2006，15，： | 1 |
| 10 | Red-light-emit-tingdiodesfabricatedbynear-ultravioletIn-aN chipswithmolybdatephosphors显示文摘 | WangZL LiangH B WangJ etal | 2006 | ApplPhysLett2006，89，7： | 1 |
| 11 | Gpx4 protects mitochondrial ATP generation against oxidative damage 显示文摘 | LiangH Remmen H V Frohlich V | 2007 | Biochemical Bio-physical Research Communications2007，356，： | 1 |
| 12 | 在背景中理解PACS发展-以中国为例[J]显示文摘 | XueY LiangH | 2007 | IEEE生物医学信息技术汇刊2007，11，1： | 1 |
| 13 | Microstructure of solution-chlorinated polyethylene by laC nuclear magnetic res onance显示文摘 | Chi Zhikuai Shi Lianghe Sheppard R N | 1984 | Polymer1984，25，36： | 1 |
| 14 | Chilling tolerance of wheat seedlings is related to an enhanced alternative respiratory pathway显示文摘 | FENG H Q LI X DUAN J G LIH Y LIANGH G | 2008 | Crop Science2008，48，： | 1 |
| 15 | XR(X23 ThI241Met polymorphism and ovarian cancer risk: a meta-analysis 显示文摘 | YanY LiangH LiR et aI | 2014 | Tumour Biol2014，35，3： | 1 |
| 16 | Themaincomponentsoftheradixofflavonoidcontentanalysis[J]显示文摘 | ZHOUXQ ZHANGQY LIANGH | | 中国中药杂志0，，： | 1 |
| 17 | Hsa‐let‐7g inhibits proliferation of hepatocellular carcinoma cells by downregulation of c‐Myc and upregulation of p16<sup>INK4A</sup>显示文摘 | Fei‐FeiLan HuaWang Yang‐ChaoChen Chu‐YanChan Samuel S.Ng KuiLi DanXie Ming‐LiangHe Marie C.Lin Hsiang‐FuKung | 2010 | Int J Cancer2010，，2： | 1 |
| 18 | Cerebral microbleeds as a predictor of 1-year outcome of poststroke depression显示文摘 | TangWK ChenY LiangH | 2014 | Stroke2014，45，： | 1 |
| 19 | Practical Strategies to Improve Test Efficiency显示文摘 | DING Zhigang WANG Hongcheng LING Lianghe | 2007 | TSINGHUA SCIENCE AND TECHNOLOGY2007，12，1： | 1 |
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