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| 1 | A transgene-free method for rapid and efficient generation of precisely edited pigs without monoclonal selection显示文摘Gene-edited pigs for agricultural and biomedical applications are typically generated using somatic cell nuclear transfer(SCNT).However, SCNT requires the use of monoclonal cells as donors, and the time-consuming and laborious monoclonal selection process limits the production of large populations of gene-edited animals. Here, we developed a rapid and efficient method named RE-DSRNP(reporter RNA enriched dual-sg RNA/CRISPR-Cas9 ribonucleoproteins) for generating gene-edited donor cells. RE-DSRNP takes advantage of the precise and efficient editing features of dual-sg RNA and the high editing efficiency, low off-target effects, transgene-free nature, and low cytotoxic characteristics of reporter RNA enriched RNPs(CRISPR-Cas9ribonucleoproteins), thus eliminating the need for the selection of monoclonal cells and thereby greatly reducing the generation time of donor cells from 3–4 weeks to 1 week, while also reducing the extent of apoptosis and chromosomal aneuploidy of donor cells. We applied RE-DSRNP to produce cloned pigs bearing a deletion edit of the wild-type p53-induced phosphatase 1(WIP1)gene: among 32 weaned cloned pigs, 31(97%) carried WIP1 edits, and 15(47%) were homozygous for the designed fragment deletion, and no off-target event was detected. The WIP1 knockout(KO) pigs exhibited male reproductive disorders, illustrating the utility of RE-DSRNP for rapidly generating precisely edited animals for functional genomics and disease research. REDSRNP's strong editing performance in a large animal and its marked reduction in the required time for producing SCNT donor cells support its application prospects for rapidly generating populations of transgene-free cloned animals. | Kui Xu Xiuling Zhang Zhiguo Liu Jinxue Ruan Changjiang Xu Jingjing Che Ziyao Fan Yulian Mu Kui Li | 2022 | Science China(Life Sciences)2022，65，8： | 9 |
| 2 | GABAergic Abnormalities Associated with Sensorimotor Corticostriatal Community Structural Deficits in ErbB4 Knockout Mice and First-Episode Treatment-Naive Patients with Schizophrenia显示文摘The current study was designed to explore how disruption of specific molecular circuits in the cerebral cortex may cause sensorimotor cortico-striatal community structure deficits in both a mouse model and patients with schizophrenia.We used prepulse inhibition(PPI)and brain structural and diffusion MRI scans in 23 mice with conditional ErbB4 knockout in parvalbumin interneurons and 27 matched controls.Quantitative real-time PCR was used to assess the differential levels of GABA-related transcripts in brain regions.Concurrently,we measured structural and diffusion MRI and the cumulative contribution of risk alleles in the GABA pathway genes in firstepisode treatment-naı¨ve schizophrenic patients(n=117)and in age-and sex-matched healthy controls(n=86).We present the first evidence of gray and white matter impairment of right sensorimotor cortico-striatal networks and reproduced the sensorimotor gating deficit in a mouse model of schizophrenia.Significant correlations between gray matter volumes(GMVs)in the somatosensory cortex and PPI as well as glutamate decarboxylase 1 mRNA expression were found in controls but not in knockout mice.Furthermore,these findings were confirmed in a human sample in which we found significantly decreased gray and white matter in sensorimotor cortico-striatal networks in schizophrenic patients.The psychiatric risk alleles of the GABA pathway also displayed a significant negative correlation with the GMVs of the somatosensory cortex in patients.Our study identified that ErbB4 ablation in parvalbumin interneurons induced GABAergic dysregulation,providing valuable mechanistic insights into the sensorimotor cortico-striatal community structure deficits associated with schizophrenia. | Chengcheng Zhang Peiyan Ni Yikang Liu Yang Tian Jinxue Wei Bo Xiang Liansheng Zhao Xiaojing Li Xiaohong Ma Wei Deng Wanjun Guo Rongjun Ni Yamin Zhang Qiang Wang Hailiang Huang Nanyin Zhang Tao Li | 2020 | Neuroscience Bulletin2020，36，2： | 9 |
| 3 | Research on Pressure Tactile Sensing Technology Based on Fiber Bragg Grating Array显示文摘一个压力触觉的传感器基于纤维布拉格栅栏(FBG ) ,数组在这篇论文,和它的有弹性的身体的数字模拟被介绍被有限元素软件(ANSYS ) 实现。根据模拟,纤维布拉格栅栏绳在灵活硅酮被植入认识到传感器制造过程,并且一个严峻的系统被造。一系列刻度测试经由高精确被做通用出版社机器。触觉的传感器数组察觉了外部压力,它被纤维栅栏解调仪器使解调,并且三尺寸的图画被规划视觉上显示位置和尺寸。同时,传感器的一个动态接触实验为模仿在未知环境遇到另外的目标的机器人被进行。试验性的结果证明传感器有好线性,重覆性,并且有动态反应的好效果,并且它的压力敏感是 0.03 nm/N。另外,传感器也有反电磁的干扰的优点,好灵活性,简单结构,低等等花费,它被期望以后在便携人工的皮肤被过去常。 | Jinxue SONG Qi JIANG Yuanyang HUANG Yibin LI Yuxi JIA Xuewen RONG Rui SONG Hongbin LIU | 2015 | Photonic Sensors2015，5，3： | 8 |
| 4 | Long-term,multidomain analyses to identify the breed and allelic effects in MSTN-edited pigs to overcome lameness and sustainably improve nutritional meat production显示文摘Beef and mutton production has been aided by breeding to integrate allelic diversity for myostatin(MSTN),but a lack of diversity in the MSTN germplasm has limited similar advances in pig farming.Moreover,insurmountable challenges with congenital lameness and a dearth of data about the impacts of feed conversion,reproduction,and meat quality in MSTN-edited pigs have also currently blocked progress.Here,in a largest-to-date evaluation of multiple MSTN-edited pig populations,we demonstrated a practical alternative edit-site-based solution that overcomes the major production obstacle of hindlimb weakness.We also provide long-term and multidomain datasets for multiple breeds that illustrate how MSTN-editing can sustainably increase the yields of breed-specific lean meat and the levels of desirable lipids without deleteriously affecting feed-conversion rates or litter size.Apart from establishing a new benchmark for the data scale and quality of genome-edited animal production,our study specifically illustrates how gene-editing site selection profoundly impacts the phenotypic outcomes in diverse genetic back-grounds. | Ziyao Fan Zhiguo Liu Kui Xu Tianwen Wu Jinxue Ruan Xinmin Zheng Shideng Bao Yulian Mu Tad Sonstegard Kui Li | 2022 | Science China(Life Sciences)2022，65，2： | 5 |
| 5 | Transportation Development Transition in China显示文摘Scientific development is an invaluable asset to a country.Policies and development modes should be carried out based on scientific findings not only in industry,but also in transportation infrastructure construction.Building an integrated transportation system,which is in line with the national requirements of China and supports sustainable socio-economic development,is a key strategic issue related to building a moderately prosperous society and achieving realistic goals of a medium-level developed country.Based on a systematic review of the advances in China's transportation infrastructure over the last 60 years,this paper explores the main drivers of transportation development,including national policy,transportation structure,investment efficiency,and technological innovation.Analysis shows that China's comprehensive transportation infrastructure construction since 1949 can be divided into five stages initiated by these drivers,which correspond to four transition modes:policy transition,structural transition,efficiency transition,and technology transition.The transition path of China's transportation development shows that the dominant factors have changed,and the interval for each transition has shortened.With the implementation of the '12th Five-Year Plan',China's transportation infrastructure construction is showing some new characteristics and facing a new development transition.Finally,this paper analyzes the trends in transportation development in China and concludes that technological innovation will be the main driving force to increase the transportation supply and service area in the future. | JIN Fengjun DING Jinxue WANG Jiao'e LIU Dong WANG Chengjin | 2012 | Chinese Geographical Science2012，22，3： | 4 |
| 6 | Work Hardening Behavior of 1020 Steel During Cold-Beating Simulation显示文摘The present research of cold-beating formation mainly focused on roller design and manufacture, kinematics, constitutive relation, metal flow law, thermo-mechanical coupling, surface micro-topography and microstructure evolution. However, the research on surface quality and performance of workpieces in the process of cold-beating is rare. Cold-beating simulation experiment of 1020 steel is conducted at room temperature and strain rates ranging from 2000 to 4000 s^(-1) base on the law of plastic forming. According to the experimental data, the model of strain hardening of 1020 steel is established,Scanning Electron Microscopy(SEM) is conducted, the mechanism of the work hardening of 1020 steel is clarified by analyzing microstructure variation of 1020 steel. It is found that the strain rate hardening effect of 1020 steel is stronger than the softening effect induced by increasing temperatures, the process of simulation cold-beating cause the grain shape of 1020 steel significant change and microstructure elongate significantly to form a fibrous tissue parallel to the direction of deformation, the higher strain rate, the more obvious grain refinement and the more hardening effect. Additionally, the change law of the work hardening rate is investigated, the relationship between dislocation density and strain, the relationship between work hardening rate and dislocation density is obtained.Results show that the change trend of the work hardening rate of 1020 steel is divided into two stages, the work hardening rate decreases dramatically in the first stage and slowly decreases in the second stage, finally tending toward zero. Dislocation density increases with increasing strain and strain rate, work hardening rate decreases with increasing dislocation density. The research results provide the basis for solving the problem of improving the surface quality and performance of workpieces under cold-beating formation of 1020 steel. | Fengkui CUI Yuanfei LING Jinxue XUE Jia LIU Yuhui LIU Yan LI | 2017 | Chinese Journal of Mechanical Engineering2017，30，2： | 2 |
| 7 | Evaluation and optimization of the spatial organization of the petrochemical industry in China显示文摘中国石油化学的工业的空间组织根据采用线性编程和 ArcGIS 的工业的开发的地位被优化空间分析工具。我们首先识别了石油化学的工业的空间组织的索引并且建立了包括四个主要范畴和 11 指示物的一个全面评估索引系统。每个索引的重量然后被分析层次过程决定。后来,作为基本单位花 337 中国人府级的政府并且科学地评估城市的潜在的全面布局系数, 151 个府级的行政单位与一个线性编程模型一起作为优化地点的选择的基础被选择。用 151 个府级的行政单位和最大范围的模型,第二,最佳的数字和精炼厂的空间分发为 100, 200 和 300 km 的服务半径被识别。就瓷器精炼厂的实际分发而言,第三,将军为精炼厂布局点和目的数字裁定价值被总结,并且 52 个精炼厂布局点为中国被选择。与 ArcGIS 空间分析工具,最后, 52 个最佳的精炼厂布局点的空间效果分别地为服务范围和社会经济的因素被模仿,并且为每个精炼厂布局点的 GDP 和人口数据然后在服务范围以内被提取。在这个基础上并且与粗略油的消费的紧张的评价,最后的结果为精制能力和乙烯生产能力的汉语的最佳的空间组织被获得。 | LIU He JIN Fengjun LIU Yi DING Jinxue XU Xu | 2013 | Journal of Geographical Sciences2013，23，1： | 2 |
| 8 | Rapid separation and idention cation of multiple constituents in traditional Chi- nese medicine formula Shenqi Fuzheng Injection by ultra-fast liquid chromatography combined with quadrupole-time-ogight mass speetrometry显示文摘 | Liu Menghua Tong Xin Wang Jinxu | 2013 | J Pharm Biomed Anal2013，74，： | 1 |
| 9 | Pressure-induced photoluminescence enhancement and ambient retention in confined carbon dots显示文摘Piezochromic luminescent materials have shown great potential in advanced optoelectronic applications.However,most of luminescent materials usually undergo emission quenching under external stimuli.Herein,we demonstrate for the first time that the photoluminescence of carbon dots(CDs)confined within sodium hydroxide can be enhanced when high pressure is applied.They exhibit a 1.6-fold fluorescence enhancement compared with pristine CDs.Importantly,the enhanced fluorescence intensity can be retained after the release of pressure to ambient conditions.A combination of experimental analysis and theoretical simulations indicates that such an enhanced emission is mainly attributed to the strong confinement resulting from the sodium hydroxide matrix,which can separate the CDs spatially and restrict the nonradiative pathway.These results provide a rational strategy for manipulating the optical properties of CDs with enhanced and retainable photoluminescence(PL)performance,thus opening up a venue for designing luminescent CDs-based materials. | Qing Lou Xigui Yang Kaikai Liu Zhongzheng Ding Jinxu Qin Yizhe Li Chaofan Lv Yuan Shang Yuewen Zhang Zhuangfei Zhang Jinhao Zang Lin Dong Chong-Xin Shan | 2022 | Nano Research2022，15，3： | 1 |
| 10 | Electron diffraction study on iron-zinc Г1 intermetallic phase in a galvannealed IF steel sheet 显示文摘 | Yu Jinshan Liu Junliang Zhang Jinxu | 2005 | Materials Letters2005，59，： | 1 |
| 11 | Molecular Dynamics Simulation and Experimental Proof of Hydrogen-enhanced Dislocation Emission in Nickel显示文摘A quasi three dimensions molecular dynamic method was used to simulate the effect of hydrogen on dislocation emission and crack propagation in nickel. In situ observation in a transmission electron microscope (TEM) was used to confirm the simulation results. The simulation result indicated that hydrogen solubilized in nickel decreased the critical stress intensity for the dislocation emission, i.e., hydrogen enhanced dislocation emission. In situ observation in TEM showed that hydrogen enhanced dislocation emission and motion before the initiation of hydrogen-induced crack. | Zhongji Li, Jinxu Li, Wuyang Chu, Hui Liu, Lyie Qiao, Materials Science and Engineering School, University of Science and Technology Beijing. Beijing 100083, China E-mail: lqiao@ustb.edu.cn | 2002 | Journal of University of Science and Technology Beijing2002，9，1： | 1 |
| 12 | Electron diffraction study on iron-zinc G1 intermetallic phase 显示文摘 | Yu Jinshan Liu Junliang Zhang Jinxu | 2005 | Mater Lett2005，59，： | 1 |
| 13 | Effects of Specific Surface Area and Impurities of Reactive Alumina on Properties of Corundum Based Castables显示文摘Effects of specific surface area and tiny amount of impurities of reactive alumina on the workability,sintering and high temperature mechanical strength of corundum based castables were investigated. The results show that the presence of reactive alumina with high specific surface area leads to accelerating of the hydration of calcium aluminate cement,thus shortening the working time and setting time of the castables,which can be ascribed that the critical energy barrier for stable nuclei of hydration products of cement can be reduced by the high specific surface area of reactive alumina. The sintering of the corundum based castables can be accelerated by the reactive alumina with high specific surface area and high amount of impurities, however, the reactive alumina with too high specific surface area and impurities can also lead to noticeable shrinkage of castables. In addition,high temperature mechanical strength of corundum based castables can be decreased by the higher amount of trace impurities of reactive alumina due to formation of lowmelting phase at high temperatures. | ZHANG Ju ZHOU Yunpeng LONG Bin ZHANG Lanyin LIU Jinxue Andreas BUHR JIA Quanli YE Guotian | 2017 | China's Refractories2017，26，4： | 1 |
| 14 | The entropy weighted non-uniform scanning algorithm for diffraction tomography显示文摘This paper studies diffraction tomography based on Fourier diffraction projection theorem. By the observation of frequency domain projecting image, one can find that projection in frequency domain is not uniformly distributed when the spatial domain scanning angle is equispaced. This results in loss of information.We analyze the conditions under which the information lost most. Then, we define the information entropy of each scanning and propose a non-uniform scanning algorithm to maximize the amount of information under conditions of a fixed number of sampling points and scanning angles. The algorithm has five different kinds of the weighting vectors based on different weighting programs. The simulation result supports our hypotheses and indicates that the algorithm proposed in this paper can obviously improve the reconstruction quality. | SUN YuLi TAO JinXu CHEN Hao LIU CongGui | 2015 | Science China(Information Sciences)2015，58，6： | 1 |
| 15 | TEM investigation of FIB induced damages in preparation of metal material TEM specimens by FIB 显示文摘 | Yu Jinshan Liu Junliang Zhang Jinxu | 2006 | Mater Lett2006，60，2： | 1 |
| 16 | Highly sensitive humidity sensors based on hexagonal boron nitride nanosheets for contactless sensing显示文摘Humidity sensors with high sensitivity,rapid response,and facile fabrication process for contactless sensing applications have received considerable attention in recent years.Herein,humidity sensors based on hexagonal boron nitride(h-BN)nanosheets that are synthesized by a facile ultrasonic process have been fabricated,which display an ultrahigh sensitivity of 28,384%at 85%relative humidity(RH),rapid response/recovery time(3.0/5.5 s),and long-term stability in a wide humidity detection range(11%-85%RH),superior to most of the reported humidity sensors.The high sensitivity can be ascribed to the massive hydrophilic functional groups absorbed on the h-BN nanosheet surface.Benefiting from the high humidity sensing performances,contactless Morse code messaging and human writing and speech recognition have been demonstrated.This work demonstrates the great potential of the high-performance h-BN nanosheet-based humidity sensors for future contactless sensing devices. | Hang Liu Jinxu Qin Xigui Yang Chaofan Lv Wentao Huang Fukui Li Chuang Zhang Yanran Wu Lin Dong Chongxin Shan | 2023 | Nano Research2023，16，7： | 0 |
| 17 | Effects of Exogenous H_(2)O_(2) on ROS Metabolism in Nitraria tangutorum Bobr. Leaves Under Salt Stress显示文摘Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of Nitralia tangutorum Bobr. under salt stress were studied. N. tangutorum seedlings were subjected to 200 mmol·L^(-1) NaCl treatment with or without the exogenous application of H_(2)O_(2) for 7 days. The results showed that NaCl stress significantly increased the relative conductivity, the contents of thiobarbituric acid reactive substances(TBARS) and ROS(H_(2)O_(2) and O_(2)^(·-)), as well as promoted the activities of antioxidant enzymes including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX) in N. tangutorum leaves. In addition, exogenous H_(2)O_(2) decreased the relative conductivity, the contents of TBARS, H_(2)O_(2) and O_(2)^(·-), while further enhanced the activities of antioxidant enzymes. These results indicated that H_(2)O_(2) effectively alleviated the adverse effects of NaCl stress on N. tangutorum through the regulation of ROS metabolism. | Zhang Nan Cahaeraduqin Sunaer Du Jinxue Pan Yiliang Wang Rui Liu Wei | 2024 | Journal of Northeast Agricultural University(English Edition)2024，31，1： | 0 |
| 18 | Applications and recent advances in transdermal drug delivery systems for the treatment of rheumatoid arthritis显示文摘Rheumatoid arthritis is a chronic,systemic autoimmune disease predominantly based on joint lesions with an extremely high disability and deformity rate.Several drugs have been used for the treatment of rheumatoid arthritis,but their use is limited by suboptimal bioavailability,serious adverse effects,and nonnegligible first-pass effects.In contrast,transdermal drug delivery systems(TDDSs)can avoid these drawbacks and improve patient compliance,making them a promising option for the treatment of rheumatoid arthritis(RA).Of course,TDDSs also face unique challenges,as the physiological barrier of the skin makes drug delivery somewhat limited.To overcome this barrier and maximize drug delivery efficiency,TDDSs have evolved in terms of the principle of transdermal facilitation and transdermal facilitation technology,and different generations of TDDSs have been derived,which have significantly improved transdermal efficiency and even achieved individualized controlled drug delivery.In this review,we summarize the different generations of transdermal drug delivery systems,the corresponding transdermal strategies,and their applications in the treatment of RA. | Yuyi Xu Ming Zhao Jinxue Cao Ting Fang Jian Zhang Yanli Zhen Fangling Wu Xiaohui Yu Yaming Liu Ji Li Dongkai Wang | 2023 | Acta Pharmaceutica Sinica B2023，13，11： | 0 |
| 19 | Enzootic epidemiology of Brucella in livestock in central Gansu Province after the National Brucellosis Prevention and Control Plan显示文摘Brucellosis remains one of the most common zoonoses spread worldwide,inducing enormous economic losses to the livestock industry and posing serious health threats to humans.Brucellosis re-emerged in China in the mid-1990s and reached a historically high level in 2015.The National Brucellosis Prevention and Control Plan(NBPCP)was initiated from 2016 to 2020.However,the present epidemiological status in livestock has not been elucidated,and whether Brucella variation occurred remains unclear.This study performed an extensive serological investigation in ruminant livestock from 2019 to 2021 in central Gansu Province,China.In total,11,296 samples from 337 farms were collected to detect the specific antibodies of Brucella.The yearly average serological prevalence of Brucella at the flock level and individual level declined from 11.32%to 8.26%and 1.17%to 0.57%,respectively.The apparent individuallevel seroprevalence of small and large ruminants was 0.89%and 0.52%,respectively.The brucellosis distribution has shifted from pastoral areas to agro-pastoral areas.Flock size and gender may be major risks of Brucella infection.Then,the B.melitensis TZ strain was isolated from female Tibetan sheep blood cell lysates.Phonotypical characterization demonstrated that it belongs to B.melitensis.biovar 3,and multilocus sequencing typing results indicated that it belongs to ST8.The whole genome and subsequent phylogenetic analysis demonstrated that the B.melitensis TZ strain is genetically more closely related to the B.melitensis QH61 strain.The B.melitensis TZ strain has similar growth characteristics to the B.melitensis 16 M strain.Overall,our study suggests that after strengthening control and prevention measures based on the NBPCP,there is a very low prevalence or absence of B.melitensis in the central Gansu Province of China,and the genotype of an epidemic strain of Brucella in Northwest China is relatively stable. | Yupeng Fang Jianjun Wang Guanyin Zhang Fengdong Zhu Chaoyue Guo Jiandong Zhang Kaixuan Guo Yun Deng Jinxue Zhang Huanchun Chen Zhengfei Liu | 2023 | Animal Diseases2023，3，2： | 0 |
| 20 | Trace doping realizing superior electrochemical performance in P2-type Na_(0.50)Li_(0.08)Mn_(0.60)Co_(0.16)Ni_(0.16)0_(2)cathode for sodium-ion batteries显示文摘P2-type layered oxides are receiving significant interest due to their superior structure and intrinsic performances.There are strenuous attempts to balance the structure stability,phase transition as well as desirable electrochemical performances by inducing anion/cation ions,changing morphology,adjusting valence,etc.In this work,several same-period elements of Sc,Ti,V,Cr,Fe,Cu and Zn are doped into Na_(0.50)Li_(0.08)Mn_(0.60)Co_(0.16)Ni_(0.16)O_(2)cathodes,which are manipulated by ions radii and valence state,further studied by operando X-ray powder diffraction patterns(XRD).As a result,the Cu^(2+)doped cathode performed higher rate capacities(as high as 86 mAh/g even at 10 C)and more stable structures(capacity retention of~89.4%for 100 cycles),which owing to the synergistic effect among the tightened TMO_(2)layer,enlarged d-spacing,reduce O-O electrostatic repulsion,ameliorate lattice distortion as well as mitigate ordering of Na^(+)/vacancy. | Hongying Hou Jinxu Qiu Bao Li Liang Wang Zhuangzhuang Zhang Mengmin Jia Xiaobing Lai Mingming Han Pengyao Yan Dai-Huo Liu Dongmei Dai Bao Wang | 2023 | Chinese Chemical Letters2023，34，12： | 0 |
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