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| 1 | β-catenin activation in hair follicle dermal stem cells in duces ectopic hair outgrowth and skin fibrosis显示文摘Hair follicle dermal sheath(DS)harbors hair follicle dermal stem cells(hfDSCs),which can be recruited to replenish DS and dermal papilla(DP).Cultured DS cells can differentiate into various cell lineages in vitro.However,it is unclear how its plasticity is modulated in vivo.Wnt/β-catenin signaling plays an important role in maintaining stem cells of various lineages and is required for HF development and regeneration.Here we report that activation ofβ-catenin in DS generates ectopic HF outgrowth(EF)by reprogramming HF epidermal cells and DS cells themselves,and endows DS cells with hair inducing ability.Epidermal homeostasis of pre-existing HFs is disrupted.Additionally,cell-autonomous progressive skin fibrosis is prominent in dermis,where the excessive fibroblasts largely originate from DS.Gene expression analysis of purified DS cells with activatedβ-catenin revealed significantly increased expression of Bmp,Fgf,and Notch ligands and administration of Bmp,Fgf,or Notch signaling inhibitor attenuates EF formation.In summary,our findings advance the current knowledge of high plasticity of DS cells and provide an insight into understanding how Wnt/β-catenin signaling controls DS cell behaviors. | Yixin Tao Qingchun Yang Lei Wang Jie Zhang Xuming Zhu Qianqian Sun Yunbin Han Qian Luo Yushu Wang Xizhi Guo Ji Wu Baojie Li Xiao Yang Lin He Gang Ma | 2019 | Journal of Molecular Cell Biology2019，11，1： | 4 |
| 2 | Long-term Fiber Photometry for Neuroscience Studies显示文摘Fiber photometry is a sensitive and easy way to detect changes in fluorescent signals. The combination of fiber photometry with various fluorescent biomarkers has substantially advanced neuroscience research over the last decade. Despite the wide use of fiber photometry in biomedical fields, the lack of a detailed and comprehensive protocol has limited progress and sometimes complicated the interpretation of data. Here, we describe detailed procedures of fiber photometry for the long-term monitoring of neuronal activity in freely-behaving animals, including surgery, apparatus setup, data collection, and analysis. | Yi Li Zhixiang Liu Qingchun Guo Minmin Luo | 2019 | Neuroscience Bulletin2019，35，3： | 3 |
| 3 | Planar morphology and controlling factors of the gullies in the Yuanmou Dry-hot Valley based on field investigation显示文摘The plane form of a gully can provide a basis for evaluating the gully volume and erosion rate, acting process, and evolutionary stage. For describing the planar characteristics of a permanent gully and understanding their controlling factors, this study, utilizing a total station and GPS RTK, measured the shoulder lines and channel curves of 112 gullies in six sites of the Yuanmou Dry-hot Valley and then mapped them by Arc GIS software and calculated nine parameters. The results showed that the channel lengths range from 10.88 to 249.11 m; the widths range from 6.20 to 40.99 m; the perimeters range from 54.11 to 541.67 m; the gully areas range from 153.02 to 6,930.30 m2; the left-side areas range from 92.93 to 4,027.20 m2; and the right-side areas range from 63.65 to 3,539.77 m2. The slightly sinuous and straight gullies account for 73.21% of the total gullies; the quantity of the right skewed gullies is 8.93% greater than that of the left skewed ones based on the symmetry ratio; the shape ratios range from 1.12 to 1.40 and the morphology ratios from 0.038 to 1.294; the fractal dimension is 1.192. Gullies in different sites have diverse planar characteristics. Except for the symmetry index, which was close to a negatively skewed distribution, all of the other parameters had the characteristic of positively skewed distribution. The gully area is related to the length and width, but the gully length has a weak correlation with the width. The evolutionary stage, topographic conditions, strata, soil properties, and piping erosion played very important roles in the gully planar morphology. This study could provide useful information for controlling gully erosion and safeguarding human habitation and engineering buildings. | DENG Qingchun MIAO Fang ZHANG Bin LUO Mingliang LIU Hui LIU Xiaojiao QIN Fachao LIU Gangcai | 2015 | Journal of Arid Land2015，7，6： | 3 |
| 4 | Primary environmental factors controlling gully distribution at the local and regional scale:An example from Northeastern China显示文摘Gully erosion is the main cause of global land degradation.The factors controlling gully erosion at watershed scale have been extensively studied,but the spatial pattern of gullies and their primary environmental factors are unclear.In order to explore the primary factors at the regional scale of over thousands of square kilometers,34 sample areas were selected in northeastern China and 10 environ-mental factors were proposed.Based on gully interpretations from high-resolution Google Earth images and spatial analysis,kernel density estimation(with polyline features),and correlation analysis,the results show that the maximum kernel density(KD)of the 34 sample areas ranged from 0.11 to 87.16,and there were significant positive correlations between the average KD and the maximum value and standard deviation.At the regional scale,topography(relief amplitude,slope,surface roughness,surface incision,and elevation with correlation coefficients(p)of 0.60,0.59,0.57,0.48,and 0.41 respectively)was the primary environmental factor,with a significant positive correlation with KD.The correlation co-efficients of the normalized difference vegetation index(NDVI)(p=032),annual precipitation(p=0.19),and soil(silt,sand,and clay content with p of 0.139,-0.117,and 0.085,respectively)decreased succes-sively with the KD.At sample area scale,there were six areas with weak correlations between envi-ronmental factors and KD,20 areas with topographic factors,six areas with annual precipitation,and two areas with NDVI as the primary factor.The correlation between soil texture and KD was low.There were different positive or negative correlations between the environmental factors and KD.The spatial dis-tribution of gullies in northeastern China is heterogeneous,and the primary environmental factors were diverse.This study will help to understand the spatial pattern and formation mechanism of gullies at large scale. | Yushu Zhou Bin Zhang Wei Qin Qingchun Deng Jun Luo Hui Liu Dan Yang Houling Wang Ying Zhao | 2021 | International Soil and Water Conservation Research2021，9，1： | 2 |
| 5 | Acoustic emission source location method and experimental verification for structures containing unknown empty areas显示文摘Acoustic emission(AE)localization plays an important role in the prediction and control of potential hazardous sources in complex structures.However,existing location methods have less discussion on the presence of unknown empty areas.This paper proposes an AE source location method for structures containing unknown empty areas(SUEA).Firstly,this method identifies the shape,size,and location of empty areas in the unknown region by exciting the active AE sources and using the collected AE arrivals.Then,the unknown AE source can be located considering the identified empty areas.The lead break experiments were performed to verify the effectiveness and accuracy of the proposed method.Five specimens were selected containing empty areas with different positions,shapes,and sizes.Results show the average location accuracy of the SUEA increased by 78%compared to the results of the existing method.It can provide a more accurate solution for locating AE sources in complex structures containing unknown empty areas such as tunnels,bridges,railroads,and caves in practical engineering. | Longjun Dong Qing Tao Qingchun Hu Sijia Deng Yongchao Chen Qiaomu Luo Xihong Zhang | 2022 | International Journal of Mining Science and Technology2022，32，3： | 2 |
| 6 | New lncRNA annotation reveals extensive functional divergence of the transcriptome in maize显示文摘Long non-coding RNAs(lncRNAs), whose sequences are approximately 200 bp or longer and unlikely to encode proteins, may play an important role in eukaryotic gene regulation. Although the latest maize(Zea mays L.) reference genome provides an essential genomic resource, genome-wide annotations of maize lncRNAs have not been updated. Here, we report on a large transcriptomic dataset collected from 749 RNA sequencing experiments across different tissues and stages of the maize reference inbred B73 line and 60 from its wild relative teosinte. We identified 18,165 high-confidence lncRNAs in maize, of which 6,873 are conserved between maize and teosinte. We uncovered distinct genomic characteristics of conserved lncRNAs,non-conserved lncRNAs, and protein-coding transcripts.Intriguingly, Shannon entropy analysis showed that conserved lncRNAs are likely to be expressed similarly to protein-coding transcripts. Co-expression network analysis revealed significant variation in the degree of co-expression. Furthermore, selection analysis indicated that conserved lncRNAs are more likely than non-conserved lncRNAs to be located in regions subject to recent selection, indicating evolutionary differentiation. Our results provide the latest genomewide annotation and analysis of maize lncRNAs and uncover potential functional divergence between proteincoding, conserved lncRNA, and non-conserved lncRNA genes, demonstrating the high complexity of the maize transcriptome. | Linqian Han Zhenna Mu Zi Luo Qingchun Pan Lin Li | 2019 | Journal of Integrative Plant Biology2019，61，4： | 2 |
| 7 | An ahemative semianalytical/analytical solution to field-to-wire coupling in an electrically large eavity显示文摘 | Zhang Huabin Zhao Xiang Luo Qingchun | 2012 | IEEE Trans- actions on Electromagnetic Compatibility2012，54，5： | 1 |
| 8 | Risk Factors for Syphilis and Prevalence of HIV, Hepatitis B and C among Men Who Have Sex with Men in Beijing, China: Implications for HIV Prevention显示文摘 | Yuhua Ruan Fengji Luo Yujiang Jia Xinxu Li Qingchun Li Hongyuan Liang Xiaoxi Zhang Dongliang Li Wei Shi Jennifer M. Freeman Sten H. Vermund Yiming Shao | 2009 | AIDS and Behavior2009，，： | 1 |
| 9 | All-fiber-transmission photometry for simultaneous optogenetic stimulation and multi-color neuronal activity recording显示文摘Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuits in vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine(DA) responses to unexpected rewards in the nucleus accumbens(NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a compatible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases. | Zhongyang Qi Qingchun Guo Shu Wang Mingyue Jia Xinwei Gao Minmin Luo Ling Fu | 2022 | Opto-Electronic Advances2022，5，12： | 0 |
| 10 | Microstructure and Tensile Properties of Spray-deposited IC6 Alloy显示文摘The microstructure and mechanical properties of a Ni3Al based Ni-Al-Mo superalloy-IC6 which possesses low impurity content and high density by spray deposition technique have been studied.The results indicate that the IC6 alloy has good tensile strength and elongation both at room and intermediate | Mi, Guofa Li, Zhou Tian, Shifan Zhao, Xianguo Luo, Zhiping Zeng,
Songyan Li, Qingchun | 1998 | Journal of Materials Science & Technology1998，14，2： | 0 |
| 11 | Boosting the Activity and Stability with Dual-Metal-N Couplings for Li–O_(2)Battery显示文摘Electrocatalysts with high efficiency are crucial for improving the storage capacity and electrochemical stability of lithium–oxygen batteries(LOBs).In this work,through a facile hydrothermal method,cobalt–nitrogen-doped carbon nanocubes(Co–N/C),the calcination products of zeolitic imidazolate framework(ZIF–67)are encapsulated by ultrathin C–MoS_(2) nanosheets to obtain Co–N/C@C–MoS_(2) composites which are used as host materials for the oxygen cathode.The synergistic effect between Co–N_(x) active sites and Mo–N coupling centers effectively promotes the formation and decomposition of Li_(2)O_(2) during repeated discharge and charge process.The mesoporous C–MoS_(2) nanosheets with delicately designed morphology facilitate charge transfer and account for improved reaction kinetics and more importantly,suppressed side reactions between the carbon materials and the electrolyte.The oxygen cathode with the Co–N/C@C–MoS_(2)host shows a high initial discharge specific capacity of 21197 mAh g^(-1)and a long operation life of 332 cycles.Theoretical calculation provides in-depth explanation for the reaction mechanism and offers insights for the rational design of electrocatalysts for LOBs. | Shan Wu Di Wu Dawei Zhang Wenhong Liu Hao Luo Jianbo He Qingchun Yang Zhiwei Li Ruiping Liu | 2022 | Energy & Environmental Materials2022，5，3： | 0 |
| 12 | A neuropsin-based optogenetic tool for precise control of Gq signaling显示文摘G_(q)-coupled receptors regulate numerous physiological processes by activating enzymes and inducing intracellular Ca^(2+)signals.There is a strong need for an optogenetic tool that enables powerful experimental control over G_(q) signaling.Here,we present chicken opsin 5(cOpn5)as the long sought-after,single-component optogenetic tool that mediates ultra-sensitive optical control of intracellular G_(q) signaling with high temporal and spatial resolution.Expressing cOpn5 in HEK 293T cells and primary mouse astrocytes enables blue light-triggered,G_(q)-dependent Ca^(2+) release from intracellular stores and protein kinase C activation.Strong Ca^(2+) transients were evoked by brief light pulses of merely 10 ms duration and at 3 orders lower light intensity of that for common optogenetic tools.Photostimulation of cOpn5-expressing cells at the subcellular and single-cell levels generated fast intracellular Ca^(2+)transition,thus demonstrating the high spatial precision of cOpn5 optogenetics.The cOpn5-mediated optogenetics could also be applied to activate neurons and control animal behavior in a circuit-dependent manner.cOpn5 optogenetics may find broad applications in studying the mechanisms and functional relevance of G_(q) signaling in both non-excitable cells and excitable cells in all major organ systems. | Ruicheng Dai Tao Yu Danwei Weng Heng Li Yuting Cui Zhaofa Wu Qingchun Guo Haiyue Zou Wenting Wu Xinwei Gao Zhongyang Qi Yuqi Ren Shu Wang Yulong Li Minmin Luo | 2022 | Science China(Life Sciences)2022，65，7： | 0 |
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