|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 3D bioprinting: an emerging technology full of opportunities and challenges显示文摘 | Bin Zhang Yichen Luo Liang Ma Lei Gao Yuting Li Qian Xue Huayong Yang Zhanfeng Cui | 2018 | Bio-Design and Manufacturing2018，1，1： | 13 |
| 2 | Anti-inflammatory activity of curcumin-loaded tetrahedral framework nucleic acids on acute gouty arthritis显示文摘Gouty arthritis is a very familiar inflammatory arthritis.Controlling inflammation is the key to preventing gouty arthritis.However,colchicine,the most highly represented drug used in clinical practice,has strict contraindications owing to some severe side effects.Curcumin(Cur),a natural anti-inflammatory drug,has demonstrated good safety and efficacy.However,the rapid degradation,poor aqueous solubility,and low bioavailability of Cur limit its therapeutic effect.To strengthen the effectiveness and bioavailability of Cur.Cur loaded tetrahedral framework nucleic acids(Cur-TFNAs)were synthesized to deliver Cur.Compared with free Cur,Cur-TFNAs exhibit a preferable drug stability,good biocompatibility(CCK-8 assay),ease of uptake(immunofluorescence),and higher tissue utilization(in vivo biodistribution).Most importantly,Cur-TFNAs present better anti-inflammatory effect than free Cur both in vivo and in vitro experiments through the determination of inflammation-related cytokines expression.Therefore,we believe that Cur-TFNAs have great prospects for the prevention of gout and similar inflammatory diseases. | Mei Zhang Xiaolin Zhang Taoran Tian Qi Zhang Yuting Wen Junyao Zhu Dexuan Xiao Weitong Cui Yunfeng Lin | 2022 | Bioactive Materials2022，7，2： | 10 |
| 3 | Crack monitoring method based on Cu coating sensor and electrical potential technique for metal structure显示文摘Advanced crack monitoring technique is the cornerstone of aircraft structural health monitoring.To achieve real-time crack monitoring of aircraft metal structures in the course of service,a new crack monitoring method is proposed based on Cu coating sensor and electrical potential difference principle.Firstly,insulation treatment process was used to prepare a dielectric layer on structural substrate,such as an anodizing layer on 2A12-T4 aluminum alloy substrate,and then a Cu coating crack monitoring sensor was deposited on the structure fatigue critical parts by pulsed bias arc ion plating technology.Secondly,the damage consistency of the Cu coating sensor and2A12-T4 aluminum alloy substrate was investigated by static tensile experiment and fatigue test.The results show that strain values of the coating sensor and the 2A12-T4 aluminum alloy substrate measured by strain gauges are highly coincident in static tensile experiment and the sensor has excellent fatigue damage consistency with the substrate.Thirdly,the fatigue performance discrepancy between samples with the coating sensor and original samples was investigated.The result shows that there is no obvious negative influence on the fatigue performance of the 2A12-T4 aluminum alloy after preparing the Cu coating sensor on its surface.Finally,crack monitoring experiment was carried out with the Cu coating sensor.The experimental results indicate that the sensor is sensitive to crack,and crack origination and propagation can be monitored effectively through analyzing the change of electrical potential values of the coating sensor. | Hou Bo He Yuting Cui Ronghong Gao Chao Zhang Teng | 2015 | Chinese Journal of Aeronautics2015，28，3： | 10 |
| 4 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 5 | Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors显示文摘Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells(PSCs)is a central aim in T cell regenerative medicine.To date,preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge.Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme(iR9-PSC)in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients.Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs,unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors.The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβrepertoire.The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice.Furthermore,gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells.This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source. | Rongqun Guo Fangxiao Hu Qitong Weng Cui Lv Hongling Wu Lijuan Liu Zongcheng Li Yang Zeng Zhijie Bai Mengyun Zhang Yuting Liu Xiaofei Liu Chengxiang Xia Tongjie Wang Peiqing Zhou Kaitao Wang Yong Dong Yuxuan Luo Xiangzhong Zhang Yuxian Guan Yang Geng Juan Du Yangqiu Li Yu Lan Jiekai Chen Bing Liu Jinyong Wang | 2020 | Cell Research2020，30，1： | 7 |
| 6 | Structural Surface Crack Monitoring Method Based on Electrical Potential Technique and Modern Surface Technology显示文摘Crack monitoring plays a great role in modern structural health monitoring, however, most of the conventional crack inspections have disadvantages in terms of the accuracy, expense, reliability, durability and level of instrumentation required. Thus, development of a simple and reliable crack inspection technique that allows continuous monitoring has been desired. In this paper, electrical potential technique and modern surface technology are employed together to develop a new structural surface crack monitoring method. A special crack monitoring coating sensor based on electrical potential technique was deposited on the hot spot of the structure by modern surface technology. The sensor consists of three layers: the isolated layer, the sensing layer and the protective layer. The isolated layer is prepared by anodic oxidation technology, the sensing layer is made of ion plated copper, and the protective layer is made of silicone. The thickness of each layer is at micrometer magnitude. The electrical conductivity of the sensor is very stable, and the fatigue performance of the specimen with or without coating sensor is nearly unchanged. The crack monitoring experiment result shows that there are two sudden rises of the coating sensor electrical potential values, corresponding to different stages of the crack initiation and propagation. Since the width of the surface coating sensor is only 0.5 mm, this crack monitoring sensor can detect the propagation of cracks less than 0.5 mm long. The method proposed takes the simplicity of electrical potential technique and can monitor surface crack of nearly all kinds of structures precisely. The results of this paper may form the basis of a new crack monitoring system. | CUI Ronghong HE Yuting YU Zhiming LI Hongpeng SHU Wenjun DU Jinqiang | 2011 | Chinese Journal of Mechanical Engineering2011，24，4： | 7 |
| 7 | Acute high-altitude hypoxic brain injury Identification of ten differential proteins显示文摘Hypobaric hypoxia can cause severe brain damage and mitochondrial dysfunction,and is involved in hypoxic brain injury.However,little is currently known about the mechanisms responsible for mitochondrial dysfunction in hypobaric hypoxic brain damage.In this study,a rat model of hypobaric hypoxic brain injury was established to investigate the molecular mechanisms associated with mitochondrial dysfunction.As revealed by two-dimensional electrophoresis analysis,16,21,and36 differential protein spots in cerebral mitochondria were observed at 6,12,and 24 hours post-hypobaric hypoxia,respectively.Furthermore,ten protein spots selected from each hypobaric hypoxia subgroup were similarly regulated and were identified by mass spectrometry.These detected proteins included dihydropyrimidinase-related protein 2,creatine kinase B-type,isovaleryl-CoA dehydrogenase,elongation factor Ts,ATP synthase beta-subunit,3-mercaptopyruvate sulfurtransferase,electron transfer flavoprotein alpha-subunit,Chain A of 2-enoyl-CoA hydratase,NADH dehydrogenase iron-sulfur protein 8 and tropomyosin beta chain.These ten proteins are all involved in the electron transport chain and the function of ATP synthase.Our findings indicate that hypobaric hypoxia can induce the differential expression of several cerebral mitochondrial proteins,which are involved in the regulation of mitochondrial energy production. | Jianyu Li Yuting Qi Hui Liu Ying Cui Li Zhang Haiying Gong Yaxiao Li Lingzhi Li Yongliang Zhang | 2013 | Neural Regeneration Research2013，8，31： | 6 |
| 8 | A male-ABCD algorithm for hepatocellular carcinoma risk prediction in HBs Ag carriers显示文摘Objective: Hepatocellular carcinoma(HCC) development among hepatitis B surface antigen(HBs Ag) carriers shows gender disparity, influenced by underlying liver diseases that display variations in laboratory tests. We aimed to construct a risk-stratified HCC prediction model for HBs Ag-positive male adults.Methods: HBs Ag-positive males of 35-69 years old(N=6,153) were included from a multi-center populationbased liver cancer screening study. Randomly, three centers were set as training, the other three centers as validation. Within 2 years since initiation, we administrated at least two rounds of HCC screening using Bultrasonography and α-fetoprotein(AFP). We used logistic regression models to determine potential risk factors,built and examined the operating characteristics of a point-based algorithm for HCC risk prediction.Results: With 2 years of follow-up, 302 HCC cases were diagnosed. A male-ABCD algorithm was constructed including participant's age, blood levels of GGT(γ-glutamyl-transpeptidase), counts of platelets, white cells,concentration of DCP(des-γ-carboxy-prothrombin) and AFP, with scores ranging from 0 to 18.3. The area under receiver operating characteristic was 0.91(0.90-0.93), larger than existing models. At 1.5 points of risk score,26.10% of the participants in training cohort and 14.94% in validation cohort were recognized at low risk, with sensitivity of identifying HCC remained 100%. At 2.5 points, 46.51% of the participants in training cohort and 33.68% in validation cohort were recognized at low risk with 99.06% and 97.78% of sensitivity, respectively. At 4.5 points, only 20.86% of participants in training cohort and 23.73% in validation cohort were recognized at high risk,with positive prediction value of 22.85% and 12.35%, respectively.Conclusions: Male-ABCD algorithm identified individual's risk for HCC occurrence within short term for their HCC precision surveillance. | Yuting Wang Minjie Wang He Li Kun Chen Hongmei Zeng Xinyu Bi Zheng Zhu Yuchen Jiao Yong Wang Jian Zhu Hui Zhao Xiang Liu Chunyun Dai Chunsun Fan Can Zhao Deyin Guo Hong Zhao Jianguo Zhou Dongmei Wang Zhiyuan Wu Xinming Zhao Wei Cui Xuehong Zhang Jianqiang Cai Wanqing Chen Chunfeng Qu | 2021 | Chinese Journal of Cancer Research2021，33，3： | 3 |
| 9 | DDIT4 mediates the proliferation-promotive effect of IL-34 in human monocytic leukemia cells显示文摘Interleukin 34(IL-34)is a cytokine that shares the receptor with colony-stimulating factor 1(CSF-1).IL-34 is involved in a broad range of pathologic processes including cancer.We previously demonstrated that IL-34 promoted the proliferation and colony formation of human acute monocytic leukemia(AMoL)cells.However,the mechanism has not been elucidated.Here,by analyzing the gene profiles of Molm13 and THP1 cells overexpressing IL-34(Molm13-IL-34 and THP1-IL-34),upregulation of the DNA damageinducible transcript 4(DDIT4)was detected in both series.Knockdown of DDIT4 effectively inhibited the proliferation,promoted apoptosis and colony formation in Molm13-IL-34 and THP1-IL-34 cells.Our results suggest that DDIT4 mediates the proliferationpromotive effect of IL-34 whereas does not mediate the promotive effect of IL-34 on colony formation in AMoL cells. | Xiaoqian Lv Yuting Hu Lina Wang Dongyue Zhang Hao Wang Yibo Dai Xiaoxi Cui Guoguang Zheng | 2021 | Blood Science2021，3，2： | 2 |
| 10 | A selected population study reveals the biochemical mechanism of intramuscular fat deposition in chicken meat显示文摘Background:Increasing intramuscular fat(IMF)is an important strategy to improve meat quality,but the regulation mechanism of IMF deposition needs to be systematically clarified.Results:A total of 520 chickens from a selected line with improved IMF content and a control line were used to investigate the biochemical mechanism of IMF deposition in chickens.The results showed that the increased IMF would improve the flavor and tenderness quality of chicken meat.IMF content was mainly determined both by measuring triglyceride(TG)and phospholipid(PLIP)in muscle tissue,but only TG content was found to be decisive for IMF deposition.Furthermore,the increase in major fatty acid(FA)components in IMF is mainly derived from TGs(including C16:0,C16:1,C18:1n9c,and C18:2n6c,etc.),and the inhibition of certain very-long-chain FAs would help to IMF/TG deposition.Conclusions:Our study elucidated the underlying biochemical mechanism of IMF deposition in chicken:Prevalent accumulation of long-chain FAs and inhibitions of medium-chain FAs and very long chain FA would jointly result in the increase of TGs with the FA biosynthesis and cellular uptake ways.Our findings will guide the production of high-quality chicken meat. | Huanxian Cui Lu Liu Xiaojing Liu Yongli Wang Na Luo Xiaodong Tan Yuting Zhu Ranran Liu Guiping Zhao Jie Wen | 2022 | Journal of Animal Science and Biotechnology2022，13，5： | 2 |
| 11 | Prepara- tion of ZnO: Eu3+ nanospheres via a micro - emulsion method and analysis of the photoluminescence properties 显示文摘 | Zhao Shihua Du Xuelian Cui Yuting | 2014 | Journal of Nanoscience and Nanotechnology2014，14，5： | 1 |
| 12 | In situ synthesis of biomass-derived Ni/C catalyst by self-reduction for the hydrogenation of levulinic acid to γ-valerolactone显示文摘Herein,we reported in situ synthesis of biomass-derived Ni/C catalyst by self-reduction with pomelo peel.Compared with the conventional method, which includes carbonization, activation, impregnation and reduction, the entire preparation process was simplified to two steps, which was more straightforward. This synthesis method was green as Ni/C can be prepared without any additional chemical and the self-reduction process was realized in N2, which can avoid using H2 thus averting some problems such as storage, transportation and safety of H2. Meanwhile, the size and dispersion of Ni particles can be controlled by changing carbonization temperature.The synthesis mechanism of Ni/C catalyst with selfreduction was investigated, which was mainly attributed to the carbon and reducing gas produced during the carbonization process.For the catalytic performance of GVL synthesis, a high yield (94.5%) can be obtained and it exhibited good stability up to 5 cycles without obvious loss of catalytic activity. | Shuqi Fang Zhibing Cui Yuting Zhu Chenguang Wang Jing Bai Xinghua Zhang Ying Xu Qiying Liu Lungang Chen Qi Zhang Longlong Ma | 2019 | Journal of Energy Chemistry2019，28，10： | 1 |
| 13 | Parallel design of convolutional neural networks for remote sensing images object recognition based on data-driven array processor显示文摘Object recognition in very high-resolution remote sensing images is a basic problem in the field of aerial and satellite image analysis.With the development of sensor technology and aerospace remote sensing technology,the quality and quantity of remote sensing images are improved.Traditional recognition methods have a certain limitation in describing higher-level features,but object recognition method based on convolutional neural network(CNN)can not only deal with large scale images,but also train features automatically with high efficiency.It is mainly used on object recognition for remote sensing images.In this paper,an AlexNet CNN model is trained using 2100 remote sensing images,and correction rate can reach 97.6%after 2000 iterations.Then based on trained model,a parallel design of CNN for remote sensing images object recognition based on data-driven array processor(DDAP)is proposed.The consuming cycles are counted.Simultaneously,the proposed architecture is realized on Xilinx V6 development board,and synthesized based on SMIC 130 nm complementary metal oxid semiconductor(CMOS)technology.The experimental results show that the proposed architecture has a certain degree of parallelism to achieve the purpose of accelerating calculations. | Shan Rui Jiang Lin Deng Junyong Cui Pengfei Zhang Yuting Wu Haoyue Xie Xiaoyan | 2020 | The Journal of China Universities of Posts and Telecommunications2020，27，6： | 1 |
| 14 | Transcriptomic Analysis of Pacific Oyster(Crassostrea gigas) Zygotes Under Hypotonic Triploid Induction显示文摘Polyploid breeding is widely used in various marine species. Low salinity treatment is an effective method of inducing triploid of bivalve mollusks. In this study, RNA-seq was performed to determine genes and pathways involved in hyposaline adaption and cell division of Pacific oyster(Crassostrea gigas) zygotes, trying to better understand the possible molecular mechanism of hypo-osmotic induction. A total of 26965 unigenes were generated in the de novo assembly of clean Illumina reads with an average length of 934 bp and N50 of 1721 bp. Of 3024 differentially expressed genes(DEGs), 2501 were up-regulated and 523 were downregulated. GO(Gene Ontology) annotation and KEGG(Kyoto Encyclopedia of Genes and Genomes) pathway analysis of these DEGs revealed that these DEGs participate a variety of biological processes including osmoregulation, cytoskeleton organization, cell survival and death, and substantially modulate cell proliferation and embryonic development. In summery, RNA-seq methodology was applied for the first time to demonstrate hypotonic-induced transcriptomic alteration in oyster zygotes. Our findings not only interpreted the relatively high mortality of induced larvae, but also provided a valuable reference for further investigations on the mechanism of hyposaline induction, thus should aid to the application of low salinity in triploid induction in large scale aquaculture in future. | LI Yangchun WANG Zhaoping CUI Yuting MA Peizhen ZHANG Xuekai FAN Chao | 2021 | Journal of Ocean University of China2021，20，1： | 1 |
| 15 | Reversible conductivity recovery of highly sensitive flexible devices by water vapor显示文摘With decreasing size of integrated circuits in wearable electronic devices,the circuit is more susceptible to aging or fracture problem,subsequently decreasing the transmission efficiency of electricity.Micro-healing represents a good approach to solve this problem.Herein,we report a water vapor method to repair microfiber-based electrodes by precise positioning and rapid healing at their original fracture sites.To realize this micro-level conducting healing,we utilize a bimaterial composed of polymeric microfibers as healing agents and electrically conductive species on its surface.This composite electrode shows a high-performance conductivity,great transparency,and ultra-flexibility.The transmittance of our electrode could reach up to 88 and 90%with a sheet resistance of 1 and 2.8Ωsq^(−1),respectively,which might be the best performance among Au-based materials as we know.Moreover,after tensile failure,water vapor is introduced to mediate heat transfer for the healing process,and within seconds the network electrode could be healed along with recovering of its resistance.The recovering process could be attributed to the combination of adhesion force and capillary force at this bimaterial interface.Finally,this functional network is fabricated as a wearable pressure/strain sensing device.It shows excellent stretchability and mechanical durability upon 1000 cycles. | Yuting Wang Yingchun Su Zegao Wang Zhongyang Zhang Xiaojun Han Mingdong Dong Lifeng Cui Menglin Chen | 2018 | npj Flexible Electronics2018，2，1： | 0 |
| 16 | 一个中央杏仁核—无名质神经环路编码厌恶增强信号(英文)显示文摘文章简介Aversive stimuli can impact motivation and support associative learning as reinforcers.However,the neural circuitry underlying the processing of aversive reinforcers has not been elucidated.Here,we report | Yuting Cui Guanghui Lv Sen Jin Jie Peng Jing Yuan Xiaobin He Hui Gong Fuqiang Xu 许彤辉 李浩洪 | 2018 | 科学新闻2018，0，4： | 0 |
| 17 | KDM2B能够依赖其招募PRC1.1复合物的功能调控体细胞重编程显示文摘文章简介PRC1(Polycomb Repressive Complex 1)复合物在发育过程中起着非常重要的作用,很多复合物成分的缺失或者异常都能直接导致胚胎发育的异常。最近的研究发现PRC1包含多种变体,但是其对细胞命运调控的具体机制并不十分清楚。 | Zhiwei Zhou Xuejie Yang Jiangping He Jing Liu Fang Wu Shengyong Yu Yuting Liu Runxia Lin He Liu Yuanbin Cui Chunhua Zhou Xiaoshan Wang Jian Wu Shangtao Cao Lin Guo Lihui Lin Tao Wang Xiaozhong Peng Boqing Qiang Andrew P.Hutchins 裴端卿 陈捷凯 | 2018 | 科学新闻2018，0，4： | 0 |
| 18 | Therapeutic application of chick early amniotic fluid: effective rescue of acute myocardial ischemic injury by intravenous administration显示文摘Myocardial regeneration has been considered a promising option for the treatment of adult myocardial injuries.Previously,a chick early amniotic fluid(ceAF)preparation was shown to contain growth-related factors that pro-moted embryonic growth and cellular proliferation,though the nature of the components within ceAF were not fully defined.Here we tested whether this ceAF preparation is similarly effective in the promotion of myocardial regen-eration,which could provide an alternative therapeutic for intervening myocardial injury.In this study,a myocardial ischemic injury model was established in adult mice and pigs by multiple research entities,and we were able to show that ceAF can efficiently rescue damaged cardiac tissues and markedly improve cardiac function in both experimental models through intravenous administration.ceAF administration increased cell proliferation and improved angio-genesis,likely via down-regulation of Hippo-YAP signaling.Our data suggest that ceAF administration can effectively rescue ischemic heart injury,providing the key functional information for the further development of ceAF for use in attenuating myocardial injury. | Baiping Cui Yufan Zheng Xiang Gao Lihong Zhang Borui Li Jia Chen Xinyan Zhou Mengyuan Cai Wenrui Sun Yuting Zhang Keejong Chang Jiayi Xu Fuyin Zhu Yan Luo Tao Sun Jin Qian Ning Sun | 2022 | Cell Regeneration2022，11，1： | 0 |
| 19 | Shipborne oceanic high-spectral-resolution lidar for accurate estimation of seawater depth-resolved optical properties显示文摘Lidar techniques present a distinctive ability to resolve vertical structure of optical properties within the upper water column at both day-and night-time.However,accuracy challenges remain for existing lidar instruments due to the ill-posed nature of elastic backscatter lidar retrievals and multiple scattering.Here we demonstrate the high performance of,to the best of our knowledge,the first shipborne oceanic high-spectral-resolution lidar(HSRL)and illustrate a multiple scattering correction algorithm to rigorously address the above challenges in estimating the depth-resolved diffuse attenuation coefficient Kd and the particulate backscattering coefficient bbp at 532 nm.HSRL data were collected during day-and night-time within the coastal areas of East China Sea and South China Sea,which are connected by the Taiwan Strait.Results include vertical profiles from open ocean waters to moderate turbid waters and first lidar continuous observation of diel vertical distribution of thin layers at a fixed station.The root-mean-square relative differences between the HSRL and coincident in situ measurements are 5.6%and 9.1%for Kd and bbp,respectively,corresponding to an improvement of 2.7-13.5 and 4.9-44.1 times,respectively,with respect to elastic backscatter lidar methods.Shipborne oceanic HSRLs with high performance are expected to be of paramount importance for the construction of 3D map of ocean ecosystem. | Yudi Zhou Yang Chen Hongkai Zhao Cedric Jamet Davide Dionisi Malik Chami Paolo Di Grolamo James H.Churnside Aleksey Malinka Huade Zhao Dajun Qiu Tingwei Cui Qun Liu Yatong Chen Sornsiri Phongphattarawat Nanchao Wang Sijie Chen Peng Chen Ziwei Yao Chengfeng Le Yuting Tao Peituo Xu Xiaobin Wang Binyu Wang Feitong Chen Chuang Ye Kai Zhang Chong Liu Dong Liu | 2022 | Light(Science & Applications)2022，11，10： | 0 |
| 20 | Molecular mechanism of lycopene cyclases regulating carotenoids ratio in different branches during tea leaf and flower development显示文摘Carotenoids are essential components in tea quality, contributing to leaf color and aroma. However, little information about carotenoids in different tea cultivars and their biosynthesis regulation mechanism during leaf development is known. Here we analyzed carotenoids by HPLC in the buds and leaves of 113 tea cultivars harvested on the same day. By profile clustering, carotenoids were divided into five groups. Same group cultivars displayed divergence in the total content of carotenoids but a similar molar ratio. To figure out the molecular mechanisms of this phenomenon, we further characterized all functional lycopene cyclases, which are the branch point of the carotenoid biosynthesis pathway. Two β-lycopene cyclases(CsLCYB1 and CsLCYB2) and one ε-lycopene cyclase(CsLCYE1) were cloned. Subcellular localization analysis showed that all cloned CsLCYs were localized in plastids. Enzyme activity assays in E. coli indicated both CsLCYBs catalyzed lycopene into β-carotene, and CsLCYE1 produced δ-carotene and ε-carotene. We found CsLCYB1 and CsLCYE1 predominantly expressed in leaf, while CsLCYB2was mainly expressed during flowering stages. Suppression by antisense oligonucleotides reduced CsLCYB1 and CsLCYE1 transcripts and led to reduction of both β,β-branch and β,ε-branch carotenoids in leaf. The expression levels of CsLCYB1 showed a significant positive correlation withβ,β-branch carotenoids in leaf. Our study provides carotenoid profiles of different tea cultivars, which can assist tea producers in selecting cultivars of interest. Meanwhile, we proposed the molecular mechanism of carotenoids reflecting the tenderness of tea plant leaf from a metabolic flux perspective, and suggested lycopene cyclase that could be applied to the breeding of tea varieties with different branch carotenoids. | Anqi Peng Xiaoyan Tang Yingying Feng Yun Huang Jilai Cui Kai Tian Mengqian Lu Yifan Zhao Yuting Pan Sanjin Wang Yutong Hu Wei Zhao Chuankui Song Qiang Wang | 2023 | Horticultural Plant Journal2023，9，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员