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| 1 | BCRP基因在非小细胞肺癌组织和非癌肺组织中的表达及意义显示文摘目的:进一步研究从人类乳腺癌细胞株 (MCF-7/AdrVp)中克隆出的、对蒽环类抗癌药耐药的 BCRP基因在非癌肺组织及非小细胞肺癌组织中的表达。方法:从术后即刻获得的正常肺组织和有活力的非小细胞肺癌组织新鲜标本中及时提取细胞总 RNA,通过 RT-PCR及 PCR法获得并扩增 BCRP基因的 cDNA产物,再经凝胶电泳分离 BCRP基因 cDNA带,然后再通过转膜技术把这些 BCRP基因 cDNA产物转移到杂交膜上,用 Southern blot杂交技术检测 BCRP基因的表达程度。结果:细胞总 RNA分别从 8个只有肺癌组织标本和 12对同时获取的肺癌组织及非癌肺组织标本中成功提取,然后用变性凝胶电泳鉴定提取 RNA的质量。其中 4例标本因术中缺血时间太长,或有坏死炎症或术前放化疗等原因,所提 RNA有降解而放弃进一步的检测。通过 RT-PCR及 PCR方法从 16例可用标本中获得并扩增 BCRP基因的 cDNA产物,再通过转膜及 Southern blot杂交技术,最终发现 BCRP基因在正常肺组织中均有不同程度的表达( 10/10),而在非小细胞肺癌组织标本中只有一半病人的标本有不同程度的表达 (8/16)。结论: BCRP基因可能是一个在非癌肺组织中常规表达的基因,而在部分病人的肺癌组织中可能此基因有缺失或被抑制。因此。 | 毛友生 Austin Doyle Weidong Yang Yuetong Wei Mark J.Krasna Douglas D.Ross | 2001 | 癌症2001，20，3： | 9 |
| 2 | Direct laser writing of volumetric gradient index lenses and waveguides显示文摘Direct laser writing(DLW)has been shown to render 3D polymeric optical components,including lenses,beam expanders,and mirrors,with submicrometer precision.However,these printed structures are limited to the refractive index and dispersive properties of the photopolymer.Here,we present the subsurface controllable refractive index via beam exposure(SCRIBE)method,a lithographic approach that enables the tuning of the refractive index over a range of greater than 0.3 by performing DLW inside photoresist-filled nanoporous silicon and silica scaffolds.Adjusting the laser exposure during printing enables 3D submicron control of the polymer infilling and thus the refractive index and chromatic dispersion.Combining SCRIBE’s unprecedented index range and 3D writing accuracy has realized the world’s smallest(15μm diameter)spherical Luneburg lens operating at visible wavelengths.SCRIBE’s ability to tune the chromatic dispersion alongside the refractive index was leveraged to render achromatic doublets in a single printing step,eliminating the need for multiple photoresins and writing sequences.SCRIBE also has the potential to form multicomponent optics by cascading optical elements within a scaffold.As a demonstration,stacked focusing structures that generate photonic nanojets were fabricated inside porous silicon.Finally,an all-pass ring resonator was coupled to a subsurface 3D waveguide.The measured quality factor of 4600 at 1550 nm suggests the possibility of compact photonic systems with optical interconnects that traverse multiple planes.SCRIBE is uniquely suited for constructing such photonic integrated circuits due to its ability to integrate multiple optical components,including lenses and waveguides,without additional printed supports. | Christian R.Ocier Corey A.Richards Daniel A.Bacon-Brown Qing Ding Raman Kumar Tanner J.Garcia Jorik van de Groep Jung-Hwan Song Austin J.Cyphersmith Andrew Rhode Andrea N.Perry Alexander J.Littlefield Jinlong Zhu Dajie Xie Haibo Gao Jonah F.Messinger Mark L.Brongersma Kimani C.Toussaint Jr Lynford L.Goddard Paul V.Braun | 2020 | Light(Science & Applications)2020，9，1： | 6 |
| 3 | Baseline effects of lysophosphatidylcholine and nerve growth factor in a rat model of sciatic nerve regeneration after crush injury显示文摘Schwann cells play a major role in helping heal injured nerves. They help clear debris, produce neurotrophins, upregulate neurotrophin receptors, and form bands of Büngner to guide the healing nerve. But nerves do not always produce enough neurotrophins and neurotrophin receptors to repair themselves. Nerve growth factor(NGF) is an important neurotrophin for promoting nerve healing and lysophosphatidylcholine(LPC) has been shown to stimulate NGF receptors(NGFR). This study tested the administration of a single intraneural injection of LPC(1 mg/mL for single LPC injection and 10 mg/mL for multiple LPC injections) at day 0 and one(day 7), two(days 5 and 7), or three(days 5, 7, and 9) injections of NGF(160 ng/mL for single injections and 80 ng/mL for multiple injections) to determine baseline effects on crush ed sciatic nerves in rats. The rats were randomly divided into four groups: control, crush, crush-NGF, and crush-LPC-NGF. The healing of the nerves was measured weekly by monitoring gait; electrophysiological parameters: compound muscle action potential(CMAP) amplitudes; and morphological parameters: total fascicle areas, myelinated fiber counts, fiber densities, fiber packing, and mean g-ratio values at weeks 3 and 6. The crush, crush-NGF, and crush-LPC-NGF groups statistically differed from the control group for all six weeks for the electrophysiological parameters but only differed from the control group at week 3 for the morphological parameters. The crush, crush-NGF, and crush-LPC-NGF groups did not differ from each other over the course of the study. Single injections of LPC and NGF one week apart or multiple treatments of NGF at 5, 7 and 9 days post-injury did not alter the healing rate of the sciatic nerves during weeks 1-6 of the study. These findings are important to define the baseline effects of NGF and LPC injections, as part of a larger effort to determine the minimal dose regimen of NGF to regenerate peripheral nerves. | Ryan L.Wood Keaton S.Karlinsey Austin D.Thompson Mark N.Rigby Greggory D.Boatright William G.Pitt Beverly L.Roeder Scott C.Steffensen Alonzo D.Cook | 2018 | Neural Regeneration Research2018，13，5： | 5 |
| 4 | Higher-risk periods for suicide among VA patients receiving depression treatment: Prioritizing suicide prevention efforts显示文摘 | Marcia Valenstein Hyungjin Myra Kim Dara Ganoczy John F. McCarthy Kara Zivin Karen L. Austin Katherine Hoggatt Daniel Eisenberg John D. Piette Frederic C. Blow Mark Olfson | 2008 | Journal of Affective Disorders2008，，： | 2 |
| 5 | Methylprednisolone and cyclophosphamide, alone or in combination, in patients with lupus nephritis 显示文摘 | Mark FGourle Y Howard A Austin Ⅲ Dorothy Scorr | 1996 | Ann Intern Med1996，125，7： | 1 |
| 6 | Use of PCR primers derived from a putative transcriptional regulator gene for species-specific determination of Listeria monocytogenes显示文摘 | Dongyou Liu A.Jerald Ainsworth Frank W. Austin Mark L. Lawrence | 2003 | International Journal of Food Microbiology2003，，3： | 1 |
| 7 | Aerobic exercise effects on neuroprotection and brain repair following stroke: A systematic review and perspective显示文摘 | Mark W. Austin Michelle Ploughman Lindsay Glynn Dale Corbett | 2014 | Neuroscience Research2014，，： | 1 |
| 8 | Bacteremia, Acute Physiology and Chronic Health Evaluation II and Modified End Stage Liver Disease are independent predictors of mortality in critically ill nontransplanted patients with acute on chronic liver failure显示文摘 | Constantine J. Karvellas Fred Pink Mark McPhail Mark Austin Georg Auzinger William Bernal Elizabeth Sizer Demetrios J. Kutsogiannis Ian Eltringham Julia A. Wendon | 2010 | Critical Care Medicine2010，，1： | 1 |
| 9 | Identification of Global DNA Methylation Signatures in Glioblastoma-Derived Cancer Stem Cells显示文摘Glioblastoma(GBM)is the most common and most aggressive primary brain tumor in adults.The existence of a small population of stem-like tumor cells that efficiently propagate tumors and resist cytotoxic therapy is one proposed mechanism leading to the resilient behavior of tumor cells and poor prognosis.In this study,we performed an in-depth analysis of the DNA methylation landscape in GBMderived cancer stem cells(GSCs).Parallel comparisons of primary tumors and GSC lines derived from these tumors with normal controls(a neural stem cell(NSC)line and normal brain tissue)identified groups of hyper- and hypomethylated genes that display a trend of either increasing or decreasing methylation levels in the order of controls,primary GBMs,and their counterpart GSC lines,respectively.Interestingly,concurrent promoter hypermethylation and gene body hypomethylation were observed in a subset of genes including MGMT,AJAP1 and PTPRN2.These unique DNA methylation signatures were also found in primary GBM-derived xenograft tumors indicating that they are not tissue culture-related epigenetic changes.Integration of GSC-specific epigenetic signatures with gene expression analysis further identified candidate tumor suppressor genes that are frequently down-regulated in GBMs such as SPINT2,NEFM and PENK.Forced re-expression of SPINT2 reduced glioma cell proliferative capacity,anchorage independent growth,cell motility,and tumor sphere formation in vitro.The results from this study demonstrate that GSCs possess unique epigenetic signatures that may play important roles in the pathogenesis of GBM. | Eun-Joon Lee Prakash Rath Jimei Liu Dungsung Ryu Lirong Pei Satish K.Noonepalle Austin Y.Shull Qi Feng N.Scott Litofsky Douglas C.Miller Douglas C.Anthony Mark D.Kirk John Laterra Libin Deng Hong-Bo Xin Xinguo Wang Jeong-Hyeon Choi Huidong Shi | 2015 | Journal of Genetics and Genomics2015，42，7： | 1 |
| 10 | Promoter Methylation and Response to Chemotherapy and Radiation in Esophageal Cancer显示文摘 | James P. Hamilton Fumiaki Sato Bruce D. Greenwald Mohan Suntharalingam Mark J. Krasna Martin J. Edelman Austin Doyle Agnes T. Berki John M. Abraham Yuriko Mori Takatsugu Kan Carmit Mantzur Bogdan Paun Suna Wang Tetsuo Ito Zhe Jin Stephen J. Meltzer | 2006 | Clinical Gastroenterology and Hepatology2006，，6： | 1 |
| 11 | Temporal trends in outcomes following sublobar and lobar resections for small (≤2 cm) non–small cell lung cancers—a Surveillance Epidemiology End Results database analysis显示文摘 | Sai Yendamuri Rohit Sharma Michael Demmy Adrienne Groman Mark Hennon Elisabeth Dexter Chukwumere Nwogu Austin Miller Todd Demmy | 2013 | Journal of Surgical Research2013，，1： | 1 |
| 12 | Velocity Adaptive Handoff Algorithms for Microcellular System显示文摘 | Mark D Austin Gordon L Stuber | 1994 | IEEE Transactions on Vehicular Technology1994，43，3： | 1 |
| 13 | An Audit Of Obsessive Compulsive Disorder in A Bedford(UK)Community Mental Health Team显示文摘 | Yi Kenwoo Austin James Agius Mark | 2014 | Psychiatria Danubina2014，26，1： | 1 |
| 14 | UK guidelines on the management of variceal haemorrhage in cirrhotic patients显示文摘 | Tripathi Dhiraj Stanley Adrian J Hayes Peter C Patch David Millson Charles Mehrzad Homoyon Austin Andrew Ferguson James W Olliff Simon P Hudson Mark Christie John M | 2015 | Gut2015，，11： | 1 |
| 15 | Frequent Mitochondrial Gene Rearrangements at the Hymenopteran nad3–nad5 Junction显示文摘 | Mark Dowton Lyda R. Castro Sarah L. Campbell Sharmilla D. Bargon Andrew D. Austin | 2003 | Journal of Molecular Evolution2003，，5： | 1 |
| 16 | Bacteremia, Acute Physiology and Chronic Health Evaluation II and Modified End Stage Liver Disease are independent predictors of mortality in critically ill nontransplanted patients with acute on chronic liver failure显示文摘 | Constantine J. Karvellas Fred Pink Mark McPhail Mark Austin Georg Auzinger William Bernal Elizabeth Sizer Demetrios J. Kutsogiannis Ian Eltringham Julia A. Wendon | 2010 | Critical Care Medicine2010，，1： | 1 |
| 17 | 拉里·佩奇:隐身的Google联合创始人显示文摘当谷歌面临生存挑战之际,拉里·佩奇似乎正在行使他被遗忘的权利。拉里·佩奇没有应约现身。谷歌的这位联合创始人和事实上的领导者因疯狂押注飞行出租车和太空升降舱而闻名,但他显然未能搭乘航班飞往华盛顿哥伦比亚特区。佩奇和Twitter的首席执行官杰克·多尔西以及Facebook的首席运营官谢丽尔·桑德伯格一起,被美国国会召唤,要他们在参议院情报委员会(SIC)面前就外国资助的行动者利用他们这些平台的方式作证。 | Mark Bergen Austin Carr | 2018 | 东西南北2018，，24： | 0 |
| 18 | 肿瘤示踪动力学分析原理(第1部分):原理和方法概述显示文摘PET可以实现局部体内肿瘤生物学的无创显像。通过设计放射性示踪剂来靶向与肿瘤相关的特定生物过程(例如肿瘤代谢、血流、增殖和肿瘤受体表达或配体结合),PET可以检测肿瘤扩散,表征肿瘤表型,并评估其对治疗的反应。例如,放射性标记的葡萄糖类似物18F-FDG糖代谢显像已被广泛用于完成上述3种任务,并在癌症护理中发挥着重要作用。然而,目前的临床实践通常是在示踪剂注射后的单个时间点进行显像(即静态显像),并没有使用PET肿瘤显像可提供的所有信息,尤其是在解决肿瘤检测以外的问题时,仅依赖从静态显像获得的示踪剂测量信息可能出现误导性结果。该篇论文由2部分组成,描述了肿瘤PET显像动力学分析的原理(第1部分),然后举例说明了肿瘤PET显像动力学分析的具体实现(第2部分)。该综述旨在介绍肿瘤显像中动力学分析的基本概念,阐明动力学分析如何增强对体内肿瘤生物学的理解,改进临床决策方法,并指导对静态图像定量测量的解释。 | Austin R.Pante Varsha Viswanath Mark Muzi Robert K.Doot David A.Mankoff 孔祥星(译) 朱华杨志(审校) | 2023 | 中华核医学与分子影像杂志2023，43，7： | 0 |
| 19 | 澳大利亚多空间创意别墅显示文摘本项目的设计宗旨在于扩大室外空间。而原建筑双正面的维多利亚式住宅存在很多问题,尤其是其有限的光照。设计师经过精心的重新布局,将现有住宅分为三部分:原住宅、新增部分和车库,三者空间通过室外空间相分隔,室内室外空间存在着一条模糊的界限. | Andrew Maynard Mark Austin Mattew McClurg Kevin Hui 张小千 | 2011 | 设计家2011，，1： | 0 |
| 20 | 澳大利亚RaeRae五口之家显示文摘它不囿于传统,但却完全符合逻辑,就如德国跑车一样,RaeRae的优美外观之下,隐藏着高度的理性主义。RaeRae是一栋位于墨尔本北菲茨罗伊的住宅,业主原先准备翻新房子的单面露台,恰逢隔壁的残损房屋出售,于是业主也将其收购,并请当地的Austin Maynard建筑设计事务所为其进行改造设计,通过翻新与合并,创建出一个带花园的五口之家住宅。 | Andrew Maynard Mark Austin Mark Strahan Kathryne Houchin Peter Bennetts(摄影) Stephanie Rooney(摄影) 范嘉苑(编译) | 2020 | 现代装饰2020，，6： | 0 |
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