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| 1 | Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage显示文摘Asparaginyl endopeptidase (AEP ) 是有为在 P1 地点的天门冬素残余的偏爱的 endo/lysosomal 半胱氨酸 endopeptidase 并且在像使用费的受体 3/7/9 的成熟起一个重要作用。AEP 被知道为催化激活在酸的 pH 经历 autoproteolytic 成熟。这里,我们描述 AEP 酶原的水晶结构和 AEP 的成熟形式。在 AEP 和 caspases 之间的结构的比较在关键残余的作文并且在催化机制揭示了类似。Mutagenesis 研究作为为肽底层的劈开是必要的残余识别了 N44, R46, H150, E189, C191, S217/S218 和 D233。在成熟期间, AEP 的帽子领域的 autoproteolytic 劈开在核心领域上开创存取到活跃地点。出人意料地,一个中间的 autoproteolytic 成熟阶段被发现在近似 pH 4.5 在哪个部分激活的 AEP 能被颠倒回到它的酶原形式。这个唯一的特征被 AEP pH4.5 (AEP 在 pH 被成熟 4.5 并且在 pH 结晶 8.5 ) ,在哪个破肽契约被重新绑扎,结构被转变回到它的酶原形式。另外, AEP 禁止者 cystatin C 能被充分激活的 AEP 消化,但是不能被激活的组织蛋白酶消化。因此,我们第一次证明 cystatins 可以为活跃地点通过底层竞争调整 AEP 的活动。 | Lixia Zhao Tian Hua Christopher Crowley Heng Ru Xiangmin Ni Neil Shaw Lianying Jiao Wei Ding Lu Qu Li-Wei Hung Wei Huang Lei Liu Keqiang Ye Songying Ouyang Genhong Cheng Zhi-Jie Liu | 2014 | Cell Research2014，24，3： | 6 |
| 2 | Structural basis for termination of AIM2-mediated signaling by p202显示文摘 | Heng Ru Xiangmin Ni LixiaZhao Christopher Crowle Wei Ding Li-Wei Hung Neil Shaw Genhong Cheng Zhi-Jie Liu | 2013 | Cell Research2013，23，6： | 5 |
| 3 | Mechanism of the Rpn13-induced activation of Uch37显示文摘 | Lianying Jiao Songying Ouyang Neil Shaw Gaojie Song Yingang Feng Fengfeng Niu Weicheng Qiu Hongtao Zhu Li-Wei Hung Xiaobing Zuo V. Eleonora Shtykova Ping Zhu Yu-Hui Dong Ruxiang Xu Zhi-Jie Liu | 2014 | Protein & Cell2014，5，8： | 4 |
| 4 | Structural Basis and Catalytic Mechanism for the Dual Functional Endo-b-N-Acetylglucosaminidase A显示文摘Endo-b-N-acetylglucosaminidases(ENGases) are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation reactions. Recently, these enzymes have | Neil Shaw | 2009 | 生物物理学报2009，25，S1： | 3 |
| 5 | Double lock of a potent human therapeutic monoclonal antibody against SARS-CoV-2显示文摘Receptor recognition and subsequent membrane fusion are essential for the establishment of successful infection by SARS-CoV-2.Halting these steps can cure COVID-19.Here we have identified and characterized a potent human monoclonal antibody,HB27,that blocks SARS-CoV-2 attachment to its cellular receptor at sub-nM concentrations.Remarkably,HB27 can also prevent SARS-CoV-2 membrane fusion.Consequently,a single dose of HB27 conferred effective protection against SARS-CoV-2 in two established mouse models.Rhesus macaques showed no obvious adverse events when administrated with10 times the effective dose of HB27.Cryo-EM studies on complex of SARS-CoV-2 trimeric S with HB27 Fab reveal that three Fab fragments work synergistically to occlude SARS-CoV-2 from binding to the ACE2 receptor.Binding of the antibody also restrains any further conformational changes of the receptor binding domain,possibly interfering with progression from the prefusion to the postfusion stage.These results suggest that HB27 is a promising candidate for immuno-therapies against COVID-19. | Ling Zhu Yong-Qiang Deng Rong-Rong Zhang Zhen Cui Chun-Yun Sun Chang-Fa Fan Xiaorui Xing Weijin Huang Qi Chen Na-Na Zhang Qing Ye Tian-Shu Cao Nan Wang Lei Wang Lei Cao Huiyu Wang Desheng Kong Juan Ma Chunxia Luo Yanjing Zhang Jianhui Nie Yao Sun Zhe Lv Neil Shaw Qianqian Li Xiao-Feng Li Junjie Hu Liangzhi Xie Zihe Rao Youchun Wang Xiangxi Wang Cheng-Feng Qin | 2021 | National Science Review2021，8，3： | 3 |
| 6 | The CT-STAT (Coronary Computed Tomographic Angiography for Systematic Triage of Acute Chest Pain Patients to Treatment) Trial显示文摘 | James A. Goldstein Kavitha M. Chinnaiyan Aiden Abidov Stephan Achenbach Daniel S. Berman Sean W. Hayes Udo Hoffmann John R. Lesser Issam A. Mikati Brian J. O’Neil Leslee J. Shaw Michael Y.H. Shen Uma S. Valeti Gilbert L. Raff | 2011 | Journal of the American College of Cardiology2011，，14： | 1 |
| 7 | Binding of bacterial secondary messenger molecule c di-GMP is a STING operation显示文摘Initial skirmishes between the host and pathogen result in spillage of the contents of the bacterial cell.Amongst the spillage,the secondary messenger molecule,cyclic dimeric guanosine monophosphate(c di-GMP),was recently shown to be bound by stimulator of interferon genes(STING).Binding of c di-GMP by STING activates the Tank Binding Kinase(TBK1)mediated signaling cascades that galvanize the body’s defenses for elimi-nation of the pathogen.In addition to c di-GMP,STING has also been shown to function in innate immune re-sponses against pathogen associated molecular pat-terns(PAMPs)originating from the DNA or RNA of pathogens.The pivotal role of STING in host defense is exemplified by the fact that STING-/-mice die upon infection by HSV-1.Thus,STING plays an essential role in innate immune responses against pathogens.This opens up an exciting possibility of targeting STING for development of adjuvant therapies to boost the im-mune defenses against invading microbes.Similarly,STING could be targeted for mitigating the inflamma-tory responses augmented by the innate immune sys-tem.This review summarizes and updates our current understanding of the role of STING in innate immune responses and discusses the future challenges in de-lineating the mechanism of STING-mediated responses. | Neil Shaw Songying Ouyang Zhi-Jie Liu | 2013 | Protein & Cell2013，4，2： | 1 |
| 8 | 人酯酶D的三维结构研究显示文摘人酯酶D(EC3.1.1.1)是非特异性酯酶家族的一员,在人体脑、肝脏、肾等器官大量表达,它的功能主要是催化水解乙酰基团。 | 武栋 李扬 宋高洁 张荣光 Neil Shaw 刘志杰 | 2009 | 生物物理学报2009，25，S1： | 1 |
| 9 | The relative risks of hyperglycaemia, obesity and dyslipidaemia in the relatives of patients with type Ⅱ diabetes mellitus显示文摘 | SHAW J T PURDIE D M NEIL H A | 1999 | Diabetologia1999，42，1： | 1 |
| 10 | The relative risks of hyperglycaemia ,obesity and dyslipidaemia in the relatives of patients with type Ⅱ diabetes mellitus 显示文摘 | SHAW J T E PURDIE D M NEIL HA W | 1999 | Diabetologia1999，42，： | 1 |
| 11 | The relative risks of hyperglycaemia in the relatives of patients with type Ⅱ diabetes mellitus显示文摘 | Shaw JTE Purdie DM Neil HAW | 1999 | Diabetologia1999，42，： | 1 |
| 12 | Vitrification of mature oocytes:improved results following addition of polyethylene glycol to a dimethyl sulfoxide solution显示文摘 | O'Neil L Paynter S J Fuller B J Shaw R W | 1997 | Cryobiology1997，34，： | 1 |
| 13 | New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16显示文摘Interferon gamma-inducible protein 16(IFI16)senses DNA in the cytoplasm and the nucleus by using two tandem hematopoietic interferon-inducible nuclear(HIN)domains,HINa and HINb,through the cooperative assembly of IFI16 filaments on double-stranded DNA(dsDNA).The role of HINa in sensing DNA is not clearly understood.Here,we describe the crystal structure of the HINa domain in complex with DNA at 2.55A°resolution and provide the first insight into the mode of DNA binding by the HINa domain.The structure reveals the presence of two oligosaccharide/nucleotide-binding(OB)folds with a unique DNA-binding surface.HINa uses loop L45 of the canonical OB2 fold to bind to the DNA backbone.The dsDNA is recognized as two single strands of DNA.Interestingly,deletion of HINb compromises the ability of IFI16 to induce IFN-b,while HINa mutants impaired in DNAbinding enhance the production of IFN-b.These results shed light on the roles of IFI16 HIN domains in DNA recognition and innate immune responses. | Xiangmin Ni Heng Ru Feng Ma Lixia Zhao Neil Shaw Yingang Feng Wei Ding Weibin Gong Qiaofeng Wang Songying Ouyang Genhong Cheng Zhi-Jie Liu | 2016 | Journal of Molecular Cell Biology2016，8，1： | 1 |
| 14 | The relative risks of hyperglycaemia, obesity and dyslipidaemia in the relatives of patients with type Ⅱ diabetes mellitus显示文摘 | Shaw JTE Purdie DM Neil HAW | 1999 | Diabetologia1999，42，： | 1 |
| 15 | The CT-STAT (Coronary Computed Tomographic Angiography for Systematic Triage of Acute Chest Pain Patients to Treatment) Trial显示文摘 | James A. Goldstein Kavitha M. Chinnaiyan Aiden Abidov Stephan Achenbach Daniel S. Berman Sean W. Hayes Udo Hoffmann John R. Lesser Issam A. Mikati Brian J. O’Neil Leslee J. Shaw Michael Y.H. Shen Uma S. Valeti Gilbert L. Raff | 2011 | Journal of the American College of Cardiology2011，，14： | 1 |
| 16 | The Nod2 Sensor Promotes Intestinal Pathogen Eradication via the Chemokine CCL2-Dependent Recruitment of Inflammatory Monocytes显示文摘 | Yun-Gi Kim Nobuhiko Kamada Michael H. Shaw Neil Warner Grace Y. Chen Luigi Franchi Gabriel Nú?ez | 2011 | Immunity2011，，5： | 1 |
| 17 | The relative risks of hyperglycaemia,obesity and dyslipidaemia in the relatives of patients with type Ⅱ diabetes mellitus显示文摘 | Shaw JT Purdie DM Neil HA | 1999 | Diabetologia1999，42，1： | 1 |
| 18 | The ever-expanding function of NOD2: autophagy, viral recognition, and T cell activation显示文摘 | Michael H. Shaw Nobuhiko Kamada Neil Warner Yun-Gi Kim Gabriel Nu?ez | 2011 | Trends in Immunology2011，，2： | 1 |
| 19 | The relative risks of hyperglycaemia, obesity and dyslipidaemia in the relatives of patients with Type II diabetes mellitus (Short Communication)显示文摘 | J. T. E. Shaw D. M. Purdie H. A. W. Neil J. C. Levy R. C. Turner | 1999 | Diabetologia1999，，1： | 1 |
| 20 | The relative risks of hyperglycaemia,obesity and dyslipidae in the relatives of patients with type 2 diabetes mellitus显示文摘 | Shaw JTE Purdie DM Neil HAW | 1999 | Diabetologia1999，42，： | 1 |
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