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| 1 | CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development显示文摘Angiogenesis,最新形成的血容器从先存在发芽的一个过程,为脊椎动物开发和成年动态平衡是重要的。以前的研究证明了 neuronal 指导分子 netrin-1 参予脉管的系统的 angiogenesis 和形态发生。Netrin-1 在 angiogenesis 展出双活动:支持或禁止 angiogenesis。netrin-1 的 anti-angiogenic 活动被 UNC5B 受体调停。然而, netrin-1 怎么支持 angiogenesis,仍然保持不清楚。这里,我们报导那 CD146,免疫球蛋白总科的 endothelial transmembrane 蛋白质,是为 netrin-1 的受体。Netrin-1 与高亲密关系绑在 CD146,导致 endothelial 房间激活并且以一种 CD146 依赖的方式的下游的发信号。在由特定的 anti-CD146 抗体的 netrin-CD146 相互作用的鼠科的内皮细胞层或混乱的 cd146 基因的有条件的大美人堵住或减少 netrin-1-induced angiogenesis。在 zebrafish 胚胎, downregulating 任何一个 netrin-1a 或 CD146 与惹人注目的类似导致脉管的缺点。而且,将宫外的脉管的发芽由 netrin-1 overexpression 导致了的 CD146 块击倒。一起,我们的数据揭开 CD146 在 angiogenesis 为 CD146 作为为 netrin-1 并且也的以前未知的受体揭示功能的 ligand,表明在脊椎动物开发期间在 angiogenesis 发信号的 netrin-CD146 的参与。 | Tao Tu Chunxia Zhang Huiwen Yan Yongting Luo Ruirui Kong Pushuai Wen Zhongde Ye Jianan Chen Jing Feng Feng Liu Jane Y Wu Xiyun Yan | 2015 | Cell Research2015，25，3： | 23 |
| 2 | 2-Methyl-5H-benzo[d]pyrazolo[5,1-b][1,3]oxazin-5-imine, an edaravone analog, exerts neuroprotective effects against acute ischemic injury via inhibiting oxidative stress显示文摘Oxidative stress plays an indispensable role in the pathogenesis of cerebral ischemia.Inhibiting oxidative stress has been considered as an effective approach for stroke treatment.Edaravone,a free radical scavenger,has been shown to prevent cerebral ischemic injury.However,the clinical efficacy of edaravone is limited because it has a low scavenging activity for superoxide anions(O_2^(·-)).Here,we report that 2-methyl-5 H-benzo[d]pyrazolo[5,1-b][1,3]oxazin-5-imine,a novel small-molecule compound structurally related to edaravone,showed a stronger inhibitory effect on oxidative stress in vitro.In vivo,2-methyl-5 H-benzo[d]pyrazolo[5,1-b][1,3]oxazin-5-imine reversed transient middle cerebral artery occlusion-induced dysfunctions of superoxide dismutases and malondialdehyde,two proteins crucial for oxidative stress,suggesting a strengthened antioxidant system.Moreover,2-methyl-5 H-benzo[d]pyrazolo[5,1-b][1,3]oxazin-5-imine decreased blood brain barrier permeability.Then,we found that 2-methyl-5 Hbenzo[d]pyrazolo[5,1-b][1,3]oxazin-5-imine had a stronger neuroprotective effect than edaravone.More importantly,2-methyl-5 H-benzo[d]pyrazolo[5,1-b][1,3]oxazin-5-imine decreased not only infarct size and neurological deficits in the acute phase but also modified neurological severity score and escape latency in Morris water maze task in the delayed period,indicating enhanced neuroprotection,sensorimotor function and spatial memory.Together,these findings suggest that 2-methyl-5 H-benzo[d]pyrazolo[5,1-b][1,3]oxazin-5-imine could be a preferable option for stroke treatment. | Huanyu Ni Yixuan Song Haiyin Wu Lei Chang Chunxia Luo Dongya Zhu | 2018 | The Journal of Biomedical Research2018，32，4： | 6 |
| 3 | Comparison of nonhuman primates identified the suitable model for COVID-19显示文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19. | Shuaiyao Lu Yuan Zhao Wenhai Yu Yun Yang Jiahong Gao Junbin Wang Dexuan Kuang Mengli Yang Jing Yang Chunxia Ma Jingwen Xu Xingli Qian Haiyan Li Siwen Zhao Jingmei Li Haixuan Wang Haiting Long Jingxian Zhou Fangyu Luo Kaiyun Ding Daoju Wu Yong Zhang Yinliang Dong Yuqin Liu Yinqiu Zheng Xiaochen Lin Li Jiao Huanying Zheng Qing Dai Qiangming Sun Yunzhang Hu Changwen Ke Hongqi Liu Xiaozhong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 4 | Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells显示文摘Phosphofructokinase-1(PFK-1),a major regulatory glycolytic enzyme,has been implicated in the functions of astrocytes and neurons.Here,we report that PFK-1 negatively regulates neurogenesis from neural stem cells(NSCs)by targeting pro-neural transcriptional factors.Using in vitro assays,we found that PFK-1 knockdown enhanced,and PFK-1 overexpression inhibited the neuronal differentiation of NSCs,which was consistent with the findings from NSCs subjected to 5 h of hypoxia.Meanwhile,the neurogenesis induced by PFK-1 knockdown was attributed to the increased proliferation of neural progenitors and the commitment of NSCs to the neuronal lineage.Similarly,in vivo knockdown of PFK-1 also increased neurogenesis in the dentate gyrus of the hippocampus.Finally,we demonstrated that the neurogenesis mediated by PFK-1 was likely achieved by targeting mammalian achaete-scute homologue-1(Mash 1),neuronal differentiation factor(NeuroD),and sex-determining region Y(SRY)-related HMG box 2(Sox2).All together,our results reveal PFK-1 as an important regulator of neurogenesis. | Fengyun Zhang Xiaodan Qian Cheng Qin Yuhui Lin Haiyin Wu Lei Chang Chunxia Luo Dongya Zhu | 2016 | Neuroscience Bulletin2016，32，3： | 4 |
| 5 | Selection and characterization of the novel anti-human PD-1 FV78 antibody from a targeted epitope mammalian cell-displayed antibody library显示文摘Currently,display-based methods are well established and widely used in antibody engineering for affinity maturation and structural stability improvement.We obtained a novel anti-human programmed death 1(PD-1)antibody using computer-aided design and a mammalian cell display technology platform.We used computer-aided modeling and distance geometry methods to predict and assign the key residues that contributed to the binding of human PD-L1 to PD-1.Then,we analyzed the sequence of nivolumab(an anti-human PD-1 antibody,referred to as MIL75 in the article)to determine the template for antibody design and library construction.We identified a series of potential substitutions on the obtained template and constructed a virtual epitope-targeted antibody library based on the physicochemical properties and each possible location of the assigned key residues.The virtual antibody libraries were displayed on the surface of mammalian cells as the antigen-binding fragments of full-length immunoglobulin G.Then,we used flow cytometry and sequencing approaches to sort and screen the candidates.Finally,we obtained a novel anti-human PD-1 antibody named FV78.FV78 competitively recognized the PD-1 epitopes that interacted with MIL75 and possessed an affinity comparable to MIL75.Our results implied that FV78 possessed equivalent bioactivity in vitro and in vivo compared with MIL75,which highlighted the probability and prospect of FV78 becoming a new potential antibody therapy. | Longlong Luo Shi Wang Xiaoling Lang Tingting Zhou Jing Geng Xinying Li Chunxia Qiao Jiannan Feng Beifen Shen Ming Lv Yan Li | 2018 | Cellular & Molecular Immunology2018，15，2： | 4 |
| 6 | Double lock of a potent human therapeutic monoclonal antibody against SARS-CoV-2显示文摘Receptor recognition and subsequent membrane fusion are essential for the establishment of successful infection by SARS-CoV-2.Halting these steps can cure COVID-19.Here we have identified and characterized a potent human monoclonal antibody,HB27,that blocks SARS-CoV-2 attachment to its cellular receptor at sub-nM concentrations.Remarkably,HB27 can also prevent SARS-CoV-2 membrane fusion.Consequently,a single dose of HB27 conferred effective protection against SARS-CoV-2 in two established mouse models.Rhesus macaques showed no obvious adverse events when administrated with10 times the effective dose of HB27.Cryo-EM studies on complex of SARS-CoV-2 trimeric S with HB27 Fab reveal that three Fab fragments work synergistically to occlude SARS-CoV-2 from binding to the ACE2 receptor.Binding of the antibody also restrains any further conformational changes of the receptor binding domain,possibly interfering with progression from the prefusion to the postfusion stage.These results suggest that HB27 is a promising candidate for immuno-therapies against COVID-19. | Ling Zhu Yong-Qiang Deng Rong-Rong Zhang Zhen Cui Chun-Yun Sun Chang-Fa Fan Xiaorui Xing Weijin Huang Qi Chen Na-Na Zhang Qing Ye Tian-Shu Cao Nan Wang Lei Wang Lei Cao Huiyu Wang Desheng Kong Juan Ma Chunxia Luo Yanjing Zhang Jianhui Nie Yao Sun Zhe Lv Neil Shaw Qianqian Li Xiao-Feng Li Junjie Hu Liangzhi Xie Zihe Rao Youchun Wang Xiangxi Wang Cheng-Feng Qin | 2021 | National Science Review2021，8，3： | 3 |
| 7 | On the Innovation of Marketing Channel Modes of China's Seed Industry显示文摘In China,the seed industry has fierce competition. The situation of homogeneous management and homogeneous products is serious. A key problem is simple and outmoded marketing channel of seed. It threatens sustainable development of seed enterprises. At present,the two-level distribution channel of seed industry has problems of similarity,low access threshold,and unfair benefit distribution,and thus urgently needs innovation. The innovation of distribution channel is inevitable requirement for promoting reorganization of China's seed industry and building of new order,is necessary weapon for dealing with trans-national seed industry competition,is essential measure for lifting the overall service level of seed industry,and is also essential means for conforming to stage development of China's agriculture. The innovation of seed marketing channel should consider changes of future agricultural development,deep-level demand of industrial market and services,scitech development level,fair distribution of industrial profit,and differentiated competition. It is recommended that channel innovation should follow the flat principle,have new and unique conception,give full play connection advantage of marginal industry,realize complementary advantages and mutual promotion,strengthen service awareness,combine with agricultural development trend,and seek sci-tech innovation of channel carrier- variety. | Bican LUO Yunhai FU Chunxia LUO Ya Zhu | 2015 | Asian Agricultural Research2015，7，5： | 1 |
| 8 | Glioma-initiating cells: A predominant role in microglia/macrophages tropism to glioma显示文摘 | Liang Yi Hualiang Xiao Minhui Xu Xianzong Ye Jun Hu Fei Li Mei Li Chunxia Luo Shicang Yu Xiuwu Bian Hua Feng | 2010 | Journal of Neuroimmunology2010，，1： | 1 |
| 9 | Predictions of Spatially Averaged Cadmium Contents in Rice Grains in the Fuyang Valley, P.R. China显示文摘 | Brus Dick J Li Zhibo Song Jing Koopmans Gerwin F Temminghoff Erwin J M Yin Xuebin Yao Chunxia Zhang Haibo Luo Yongming Japenga Jan | 2009 | Journal of Environmental Quality2009，，3： | 1 |
| 10 | 20-Hydroxyeedysone protects against oxidative stress-induced neuronal injury by scaveng- ing free radicals and medulatitag NF- K B and JNK pathways 显示文摘 | Hu Jun Luo Chunxia Chu Weihua | 2012 | PLoSOne2012，7，50： | 1 |
| 11 | Characterizing iron deposition in Parkinson’s disease using susceptibility-weighted imaging: An in vivo MR study显示文摘 | Jiuquan Zhang Yanling Zhang Jian Wang Ping Cai Chunxia Luo Zhongming Qian Yongming Dai Hua Feng | 2010 | Brain Research2010，，： | 1 |
| 12 | A novel oncolytic adenovirus targeting to telomerase activity in tumor cell with potent显示文摘 | Zou Weiguo Luo Chunxia Zhang Zilai | 2004 | Oncogene2004，23，： | 1 |
| 13 | Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice显示文摘Ulcerative colitis(UC)is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.Previous studies have indicated that celastrol(CSR)has strong anti-inflammatory and immune-inhibitory effects.Here,we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model,and addressed the mechanism by which CSR exerts the protective effects.We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining,intestinal permeability assay,cytokine assay,flow cytometry,fecal microbiota transplantation(FMT),16S rRNA sequencing,untargeted metabolomics,and cell differentiation.CSR administration significantly ameliorated the dextran sodium sulfate(DSS)-induced colitis in mice,which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index(DAI)score and intestinal permeability.Meanwhile,CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels,and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.Notably,all the therapeutic effects were exerted in a gut microbiota-dependent manner.Furthermore,CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites,which is probably associated with the gut microbiota-mediated protective effects.In conclusion,this study provides the strong evidence that CSR may be a promising therapeutic drug for UC. | Mingyue Li Weina Guo Yalan Dong Wenzhu Wang Chunxia Tian Zili Zhang Ting Yu Haifeng Zhou Yang Gui Kaming Xue Junyi Li Feng Jiang Alexey Sarapultsev Huafang Wang Ge Zhang Shanshan Luo Heng Fan Desheng Hu | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 1 |
| 14 | A spike-trimer protein-based tetravalent COVID-19 vaccine elicits enhanced breadth of neutralization against SARS-CoV-2 Omicron subvariants and other variants显示文摘Multivalent vaccines combining crucial mutations from phylogenetically divergent variants could be an effective approach to defend against existing and future SARS-Co V-2 variants.In this study,we developed a tetravalent COVID-19 vaccine SCTV01E,based on the trimeric Spike protein of SARS-Co V-2 variants Alpha,Beta,Delta,and Omicron BA.1,with a squalenebased oil-in-water adjuvant SCT-VA02B.In the immunogenicity studies in na?ve BALB/c and C57BL/6J mice,SCTV01E exhibited the most favorable immunogenic characteristics to induce balanced and broad-spectrum neutralizing potencies against pre-Omicron variants(D614G,Alpha,Beta,and Delta)and newly emerging Omicron subvariants(BA.1,BA.1.1,BA.2,BA.3,and BA.4/5).Booster studies in C57BL/6J mice previously immunized with D614G monovalent vaccine demonstrated superior neutralizing capacities of SCTV01E against Omicron subvariants,compared with the D614G booster regimen.Furthermore,SCTV01E vaccination elicited na?ve and central memory T cell responses to SARS-Co V-2 ancestral strain and Omicron spike peptides.Together,our comprehensive immunogenicity evaluation results indicate that SCTV01E could become an important COVID-19 vaccine platform to combat surging infections caused by the highly immune evasive BA.4/5 variants.SCTV01E is currently being studied in a head-to-head immunogenicity comparison phase 3 clinical study with inactivated and m RNA vaccines(NCT05323461). | Rui Wang Hongpeng Huang Chulin Yu Chunyun Sun Juan Ma Desheng Kong Yalong Lin Dandan Zhao Shaozheng Zhou Jianbo Lu Sai Cao Yanjing Zhang Chunxia Luo Xuefeng Li Yang Wang Liangzhi Xie | 2023 | Science China(Life Sciences)2023，66，8： | 1 |
| 15 | Research on Modal Parameters Identification of Parallel Manipulator with Flexible Multi-Body System显示文摘 | Zhu Chunxia Luo Jiman Wang Dan | 2013 | Research Journal of Applied Sciences2013，5，10： | 1 |
| 16 | Characterizing iron deposition in Parkinson’s disease using susceptibility-weighted imaging: An in vivo MR study显示文摘 | Jiuquan Zhang Yanling Zhang Jian Wang Ping Cai Chunxia Luo Zhongming Qian Yongming Dai Hua Feng | 2010 | Brain Research2010，，： | 1 |
| 17 | Brasenia-inspired hydrogel with sustained and sequential release of BMP and WNT activators for improved bone regeneration显示文摘Although bone morphogenetic protein(BMP) and WNT signaling play pivotal roles in bone development,homeostasis, and regeneration, the applications of proteins to stimulate corresponding signaling pathways showed limited outcomes in the repair and regeneration of bone defects that might be attributed to the reciprocal interventions of these pathways. In order to satisfy the combinational and sequential activation of BMP and WNT pathways, inspired by the heterogeneous hydrogel-liked structures of Brasenia, heterogeneous alginate/chitosan hydrogels were fabricated and spatially loaded with FK506 and BIO to achieve sustained and sequential release of the activators. Alkaline phosphatase staining, alizarin red staining and q RT-PCR results suggested that FK506 and BIO enhanced osteoblastic differentiation in vitro when used separately. Besides, by mixing and matching the activators and the hydrogel layers, a superior releasing mode that a combination of early FK506 release and following BIO release was identified via both in vitro and in vivo explorations for most efficient bone regeneration. These results suggested that drug-loaded heterogeneous hydrogels possess great potentials in treating bone loss defects for future clinical practice. | Xinqing Hao Xuewei Zhang Yue Hu Chunxia Ren Cangwei Liu Lu Wang Yijun Zhou Shuangshuang Wang Huanyu Luo Guangxing Yan Xiao Wang Xiaomeng Wang Feilong Ren Ce Shi, Wenlong Song Hongchen Sun | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
| 18 | High-Affinity Decoy PD-1 Mutant Screened from an Epitope-Specific Cell Library显示文摘Immunotherapy with anti-programmed cell death protein-1(PD-1)/programmed cell death ligand-1(PDL1)monoclonal antibodies has become routine in the treatment of many kinds of human cancers,such as lung cancer,intestinal cancer,and melanoma.The PD-1/PD-L1 pathway inhibits T cell activation in the micro-environment,making it an attractive target against cancer.Wild-type(WT)PD-1 ectodomain has been shown to have difficulty blocking PD-1/PD-L1 mixture formation due to its low affinity.The present work uses three-dimensional(3D)crystal complex structures to analyze the interaction by which PD-1 binds to PD-L1 or PD-L2.It also reports on a theoretical study of the binding mode between PD-1 and its clinical antibody Opdivo.Based on the theoretical binding analysis of PD-1 and its ligands(i.e.,PD-L1 and PD-L2)or antibody(Opdivo),a small-content,epitope-oriented mammalian cell library was established for PD-1.After three rounds of cell sorting,the decoy PD-1 mutant 463,which presented a higher affinity than WT PD-1 to the PD-L1(the affinity has increased by almost three orders of magnitude)was screened out.It exhibited an inhibitory effect against PD-1 to prevent it from forming mixtures with PD-L1,which was similar to the effect of the commercial anti-PD-L1 antibody atezolizumab(ATE).The median effective concentration(EC50)value of the decoy mutant was 0.031 μg·mL^(-1) in comparison with 0.063 μg·mL^(-1) for ATE;both values were much lower than that of WT PD-1,at 2.571 μg·mL^(-1).The 463 decoy mutant reversed the inhibitory function of PD-1 in T cell activation;furthermore,10 mg·kg^(-1) of 463 inhibited about 75%of tumor growth in vivo in a MC38 transgenic xenograft mice model,which was similar to the activity of ATE.More interestingly,an even lower dose of 463(2 mg·kg^(-1))showed a better effect than 10 mg·kg^(-1) of WT PD-1.This work offers the decoy 463 with an improved curative effect,which holds potential to become a good option against PD-1/PD-L1-related cancers. | Hao Liu Chunxia Qiao Naijing Hu Zhihong Wang Jing Wang Jiannan Feng Beifen Shen Yuanfang Ma Longlong Luo | 2021 | Engineering2021，7，11： | 0 |
| 19 | The olfactory route is a potential way for SARS-CoV-2 to invade the central nervous system of rhesus monkeys显示文摘Neurological manifestations are frequently reported in the COVID-19 patients.Neuromechanism of SARS-CoV-2 remains to be elucidated.In this study,we explored the mechanisms of SARS-CoV-2 neurotropism via our established non-human primate model of COVID-19.In rhesus monkey,SARS-CoV-2 invades the CNS primarily via the olfactory bulb.Thereafter,viruses rapidly spread to functional areas of the central nervous system,such as hippocampus,thalamus,and medulla oblongata.The infection of SARS-CoV-2 induces the inflammation possibly by targeting neurons,microglia,and astrocytes in the CNS.Consistently,SARS-CoV-2 infects neuro-derived SK-N-SH,glial-derived U251,and brain microvascular endothelial cells in vitro.To our knowledge,this is the first experimental evidence of SARS-CoV-2 neuroinvasion in the NHP model,which provides important insights into the CNS-related pathogenesis of SARS-CoV-2. | Li Jiao Yun Yang Wenhai Yu Yuan Zhao Haiting Long Jiahong Gao Kaiyun Ding Chunxia Ma Jingmei Li Siwen Zhao Haixuan Wang Haiyan Li Mengli Yang Jingwen Xu Junbin Wang Jing Yang Dexuan Kuang Fangyu Luo Xingli Qian Longjiang Xu Bin Yin Wei Liu Hongqi Liu Shuaiyao Lu Xiaozhong Peng | 2021 | Signal Transduction and Targeted Therapy2021，6，5： | 0 |
| 20 | Application of eDNA Metabarcoding for Detecting Anura in North China显示文摘eDNA metabarcoding is an advanced method formonitoring biodiversityproposed in recent years.By analyzing DNA in water,soil and sediment samples,the technology obtains species distribution and population quantity information.It was found that macrobarcode technology is more accurate than the traditional method in measuring the species richness of some groups.In Europe,America and South America,the relia bility of this technology in monitoring amphibian diversity in the wild was studied,and it was found to be better than traditional biodiversity monitoring methods in detecting species diversity.At present,amphibian monitoring mainly depends on various traditional methods,such as transects,drift fence traps,artificial shelters and mark-recapture.These monitoring techniques have many shortcomings,such as low accuracy and strong subjectivity of study results.These technologies have poor effects on rare,invasive and endangered species with strong concealment ability,low density and strong seasonality and are difficult to implement in sites inaccessible to people.Traditional monitoring technology also requires considerable investment of human and material resources,and the economic cost is relatively high,while eDNA metabarcoding ismore efficient and less costly,so it is important to use eDNA meta barcoding in amphibian monitoring in China.In this study,the eDNA meta barcoding and traditional line transect method(TLTM)were used to study the characteristics of the two methods in the Beijing-Tianjin-Hebeiregion.Repeated samplingwas conducted on 58 waterbodies in July 2019 and June 2020.After sequencing the samples using highthroughput sequencing technology,the differences between metabarcoding and commonly used TLTM surveys in detecting the diversity of four amphibians in North China were assessed.Our results showed that eDNA meta barcoding is more sensitive to the detection of the four amphibian species in the sampling area,and the combined use of eDNA metabarcoding and TLTM can improve the survey results of amphibians in the survey area to the greatest extent.In addition,in the process of species classification and identification of metabarcoding results,7 species of reptiles were detected,indicating that eDNA metabarcoding is also useful to detect reptiles.The results of this study indicate that metabarcoding in combination with TLTM can accurately estimate the diversityof amphibians in a short-term survey in North China and is also useful in reptile species detection. | Wenhao LI Mingshuo QIN Xianglei HOU Jiaqi ZHANG Siqi WANG Yu LI Zexu LUO Teng DENG Tianjian SONG Chunxia XU Xuan LIU Xuyu WANG Yiming LI | 2022 | Asian Herpetological Research2022，13，4： | 0 |
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