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| 1 | 藁本内酯对神经干细胞/祖细胞的增殖作用及机制研究显示文摘目的研究藁本内酯对神经干/祖细胞(neural stem/progenitor cells,NS/PCs)的增殖作用及机制。方法孕14 d Wistar大鼠脱颈椎处死,分离胎鼠脑皮层部位NS/PCs,采用悬浮法原代培养7 d。采用WST-1法检测藁本内酯对NS/PCs存活率的影响;采用Western blotting法检测藁本内酯对NS/PCs增殖相关蛋白磷酸化蛋白激酶B(phosphorylated protein kinase B,p-Akt)、Akt、磷酸化糖原合成酶激酶(phosphorylated glycose synthase kinase 3β,p-GSK3β)、GSK3β和active-β-catenin表达;给予藁本内酯第1-3天,观察NS/PCs神经球生长状态并测量其直径;分化培养基诱导NS/PCs分化5 d后,采用免疫荧光法检测藁本内酯对NS/PCs分化类型的影响,并采用WST-1法检测藁本内酯对分化细胞存活率的影响。结果藁本内酯(25μmol/L)显著促进NS/PCs存活率(P<0.05),显著上调增殖相关蛋白p-Akt/Akt和active-β-catenin表达(P<0.05);给予藁本内酯第1-3天,可增加NS/PCs神经球直径,但无统计学差异;藁本内酯对神经元、星型胶质细胞方向分化无显著影响,但分化后细胞存活率显著升高(P<0.05)。结论藁本内酯可促进NS/PCs增殖,其作用机制与Akt/β-catenin通路有关。藁本内酯可能对神经元、星型胶质细胞以外的其他分化类型细胞有干预作用。 | 王敏 Hideki Hayashi 刘建勋 Norio Takagi 任钧国 | 2021 | 中草药2021，52，20： | 2 |
| 2 | Overexpression of NK2 inhibits liver regeneration after partial hepatectomy in mice显示文摘AIM: To investigate the in vivo effects of NK2 on liver regeneration after partial hepatectomy (PH). METHODS: Survival after PH was observed with 21 NK2 transgenic mice and 23 wild-type (WT) mice over 10 d. Liver regeneration was analyzed using histology and immunohistochemistry. Expressions of genes were analyzed using Northern blot analysis, immunoprecipitation and immunoblotting, and reverse transcriptase polymerase chain reaction assay. KaplanMeier method and the log-rank test were used for ahalyzing the survival after PH. Differences in the resultsof immunohistochemistry and percentage of liver regeneration was determined by the Student's t-test. RESULTS: More than half of NK2 transgenic mice died within 48 h after PH. After PH, increased deposition of small lipid droplets in hepatocytes was evident and hepatic proliferation was inhibited in NK2 transgenic mice. The hepatic expression and kinase activity of HGF receptor, c-Met, were unchanged among WT mice and NK2 transgenic mice after PH. The expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in liver tissues were prolonged in NK2 transgenic mice that died after PH.CONCLUSION: Our findings indicate that overexpression of NK2 inhibits liver regeneration after PH. | Toshiyuki Otsuka Norio Horiguchi Daisuke Kanda Takashi Kosone Yuichi Yamazaki Kazuhisa Yuasa Naondo Sohara Satoru Kakizaki Ken Sato Hitoshi Takagi Glenn Merlino Masatomo Mori | 2005 | World Journal of Gastroenterology2005，11，47： | 2 |
| 3 | Clinical characteristics of null responders to Peg-IFNα2b/ ribavirin therapy for chronic hepatitis C显示文摘AIM: To predict which chronic hepatitis C patients are likely to be late-responders, we herein investigated the clinical characteristics of null-responders at 36 wk with hepatitis C virus (HCV) genotype Ib and a high viral load during the course of pegylated interferon (Peg-IFN)/ ribavirin ther apy. METHODS: One hundred forty-two patients with genotype Ib HCV and a high viral load were included in this study. Peg-IFNα2b (1.5 μg/kg once a week) and ribavirin (600-1000 mg per day according to body weight) were administered for 48 wk. We def ined nullresponders as the cases that never cleared serum HCV RNA as determined using RT-PCR until 36 wk. Other patients were def ined as responders. We compared the clinical characteristics (age, gender, body mass index, previous treatment) and HCV RNA titer during the therapy between null-responders and responders.RESULTS: The HCV RNA clearance rate was 17.9% (24/134), 46.3% (62/134), 60.6% (86/142), 86.6% (123/142), and 88.0% (125/142) at 4, 8, 12, 24, and 36 wk, respectively. There were 17 patients (12.0%) who were still null-responders at 36 wk. There were no differences in the clinical characteristics between the responders and null-responders except for the titer and decline rates of HCV RNA at 1 wk and 4 wk. The HCV RNA titers at 1 wk and after 4 wk of treatment were significantly higher in the null-responders in comp arison to the responders (P <0.01). The serum HCV RNA titers of the responders decreased by 1.3 log after 1 wk of treatment, and 1.6 log after 4 wk of treatm ent, respectively. On the other hand, the titers of the null responders decreased by only 0.5 log after 1 wk, and 0.7 log after 4 wk of treatment, respectively. The decrease rates of HCV RNA after 1 and 4 wk of treatm ent were signif icantly worse for null responders than for the responders (P <0.01). CONCLUSION: The HCV RNA titer at 1 wk and 4 wk after initiating treatment may be useful for predicting null-responders to Peg-IFNα2b/ribavirin therapy. However, further investigation is needed to determine the optimal time at which the decision to discontinue the Peg-IFNα2b/ribavirin therapy for null-responders can be made. | Hideyuki Suzuki Satoru Kakizaki Norio Horiguchi Takeshi Ichikawa Ken Sato Hitoshi Takagi Masatomo Mori | 2010 | World Journal of Hepatology2010，2，11： | 2 |
| 4 | Hepatitis B Virus Gene in Liver Tissue Promotes Hepatocellular Carcinoma Development in Chronic Hepatitis C Patients显示文摘 | Shin-Ichi Fujioka Hiroyuki Shimomura Yoshiaki Iwasaki Kozo Fujio Hiroshi Nakagawa Yasuhiro Onishi Shinjiro Takagi Hideaki Taniguchi Fumi Umeoka Hirofumi Nakajima Akio Moriya Katsuyuki Nanba Cheng-Yu Piao Toshiyuki Shinji Norio Koide Yasush Shiratori | 2003 | Digestive Diseases and Sciences2003，，10： | 1 |
| 5 | The effects of monobromobimane on neuronal cell death in the hippocampus after transient global cerebral ischemia in rats 显示文摘 | Tsutomu Abe Norio Takagi Midori Nakano | 2004 | Neuroscience Letters2004，357，： | 1 |
| 6 | Lackof macrophage inhibitor factor protects mice against concanavalinA-induced liver injury显示文摘 | Hiroaki Nakajima Hitoshi Takagi Norio Horiguchi | 2006 | Liver International2006，26，3： | 1 |
| 7 | Lackof macrophage inhibitor factor protects mice against concanavalinA - induced liver injury 显示文摘 | Hiroaki Nakajima Hitoshi Takagi Norio Hofiguchi | 2006 | Liver International2006，26，3： | 1 |
| 8 | Low rate of YMDD motif mutations in polymerase gene of hepatitis B virus in chronically infected patients not treated with lamivudine显示文摘 | Marie Matsuda Fumitaka Suzuki Yoshiyuki Suzuki Akihito Tsubota Norio Akuta Tetsuya Hosaka Takashi Someya Masahiro Kobayashi Satoshi Saitoh Yasuji Arase Junko Satoh Kimiko Takagi Mariko Kobayashi Kenji Ikeda Hiromitsu Kumada | 2004 | Journal of Gastroenterology2004，，1： | 1 |
| 9 | Intravenous injectionof neural progenitor cells facilitates angiogenesis after cerebral ischemia 显示文摘 | Yoshiyuki Moriyama Norio Takagi Kanae Hashimura | 2013 | Brain Behav2013，3，2： | 1 |
| 10 | Lack of mac- mphage migration inhibitory factor protects mice against concanavalin A-induced liver injury 显示文摘 | Hiroaki Nakajima Hitoshi Takagi Norio Horiguehi | 2006 | Liver International2006，26，3： | 1 |
| 11 | Fibrous dysplasia in the maxilla:possible mechanism of bone remodeling by calcitonin treatment显示文摘 | Tadashi Yasuoka Norio Takagi Daijiro Hatakeyama | 2003 | Oral Oncology2003，39，： | 1 |
| 12 | Hepatocyte growth factor attenuates cerebral ischemia-induced increasein permeability of the blood-brain barrier and decreases in expression of tight junctional proteins in cerebral vessels显示文摘 | Ichiro Date Norio Takagi Keiko Takagi | 2006 | Neuroscience Letters2006，407，： | 1 |
| 13 | Low rate of YMDD motif mutations in polymerase gene of hepatitis B virus in chronically infected patients not treated with lamivudine显示文摘 | Marie Matsuda Fumitaka Suzuki Yoshiyuki Suzuki Akihito Tsubota Norio Akuta Tetsuya Hosaka Takashi Someya Masahiro Kobayashi Satoshi Saitoh Yasuji Arase Junko Satoh Kimiko Takagi Mariko Kobayashi Kenji Ikeda Hiromitsu Kumada | 2004 | Journal of Gastroenterology2004，，1： | 1 |
| 14 | Alterations in hippocampal GAP-43, BDNF, and L1 following sustained cerebral ischemia显示文摘 | Keiko Miyake Wataru Yamamoto Mina Tadokoro Norio Takagi Kyoko Sasakawa Atsumi Nitta Shoei Furukawa Satoshi Takeo | 2002 | Brain Research2002，，1： | 1 |
| 15 | Role of nuclear receptor CAR in carbon tetrachloride-induced hepatotoxicity显示文摘AIM: To investigate the precise roles of CAR in CCl4-induced acute hepatotoxicity.METHODS: To prepare an acute liver injury model, CCl4 was intraperitoneally injected in CAR+/+ and CAR-/- mice.RESULTS: Elevation of serum alanine aminotransferase and extension of centrilobular necrosis were slightly inhibited in CAR-/- mice compared to CAR+/+ mice without PB. Administration of a CAR inducer, PB, revealed that CCl4-induced liver toxicity was partially inhibited in CAR-/- mice compared with CAR+/+ mice. On the other hand,androstanol, an inverse agonist ligand, inhibited hepatotoxicity in CAR+/+ but not in CAR-/- mice. Thus, CAR activation caused CCl4 hepatotoxicity while CAR inhibition resulted in partial protection against CCl4-induced hepatotoxicity.There were no differences in the expression of CYP2E1, the main metabolizing enzyme for CCl4, between CAR+/+ and CAR-/- mice. However, the expression of other CCl4-metabolizing enzymes, such as CYP2B10 and 3A11, was induced by PB in CAR+/+ but not in CAR-/- mice. Although the main pathway of CCl4-induced acute liver injury is mediated by CYP2E1, CAR modulates its pathway via induction of CYP2B10 and 3A11 in the presence of activator or inhibitor.CONCLUSION: The nuclear receptor CAR modulates CCl4-induced liver injury via induction of CCl4-metabolizing enzymes in the presence of an activator. Our results suggest that drugs interacting with nuclear receptors such as PB might play critical roles in drug-induced liver injury or drugdrug interaction even though such drugs themselves are not hepatotoxic. | Yuichi Yamazaki Satoru Kakizaki Norio Horiguchi Hitoshi Takagi Masatomo Mori Masahiko Negishi | 2005 | World Journal of Gastroenterology2005，11，38： | 1 |
| 16 | A Case of Superior Oblique Myokymia Observed by an Image-analysis System显示文摘 | Yuuki Hayakawa Mineo Takagi Hiruma Hasebe Shigeru Hasegawa Ritsuko Takada Tomoaki Usui Haruki Abe Kikuo Shibasaki Hiroshi Yaoeda Kazuhiko Ukai Norio Ishikawa | 2000 | Journal of Neuro-Ophthalmology2000，，3： | 1 |
| 17 | Effect of NMDA receptor antagonist on proliferation of neurospheres from embryonic brain显示文摘 | Nobuyuki Mochizuki Norio Takagi Koji Kurokawa Takayuki Kawai Shintaro Besshoh Kouichi Tanonaka Satoshi Takeo | 2007 | Neuroscience Letters2007，，2： | 1 |
| 18 | The effects of monobromobimane on neuronal cell death in the hippocampus after transient global cerehral ischemia in rats显示文摘 | Tsutomu Abe Norio Takagi Midori Nakano | 2004 | Neuroscience Letters2004，357，： | 1 |
| 19 | The effects of monobromobimane on neuronal cell death in the hippocampus after transient global cerebral ischemia in rats显示文摘 | Norio Takagi Midori Nakano | 2004 | Neuroscience Letters2004，357，： | 1 |
| 20 | The effects of monobromobimane on neuronal cell death in the hippocampus after transient global cerebral ischemia in rats显示文摘 | Tsutomu Abe Norio Takagi Midori Nakano | 2004 | Neuroscience Letters2004，357，： | 1 |
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