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| 1 | Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation显示文摘ABSTRACTHuman placenta-derived mononuclear cells (MNC) were isolated by a Percoll density gradient and cultured in mesen-chymal stem cell (MSC) maintenance medium. The homogenous layer of adherent cells exhibited a typical fibroblast-like morphology, a large expansive potential, and cell cycle characteristics including a subset of quiescent cells. In vitrodifferentiation assays showed the tripotential differentiation capacity of these cells toward adipogenic, osteogenic andchondrogenic lineages. Flow cytometry analyses and immunocytochemistry stain showed that placental MSC was ahomogeneous cell population devoid of hematopoietic cells, which uniformly expressed CD29, CD44, CD73, CD105,CD166, laminin, fibronectin and vimentin while being negative for expression of CD31, CD34, CD45 and α-smoothmuscle actin. Most importantly, immuno-phenotypic analyses demonstrated that these cells expressed class I majorhistocompatibility complex (MHC-I), but they did not express MHC-II molecules. Additionally these cells could sup-press umbilical cord blood (UCB) lymphocytes proliferation induced by cellular or nonspecific mitogenic stimuli. Thisstrongly implies that they may have potential application in allograft transplantation. Since placenta and UCB arehomogeneous, the MSC derived from human placenta can be transplanted combined with hematopoietic stem cells(HSC) from UCB to reduce the potential graft -versus-host disease (GVHD) in recipients. | Chang Dong LI Wei Yuan ZHANG He Lian LI Xiao Xia JIANG Yi ZHANG Pei Hsien TANG Ning MAO | 2005 | Cell Research2005，15，7： | 36 |
| 2 | The Serum Exosome Derived MicroRNA-135a,-193b, and-384 Were Potential Alzheimer's Disease Biomarkers显示文摘Objective MicroR NAs(mi Rs) are attractive molecules to be considered as one of the blood-based biomarkers for neurodegenerative disorders such as Alzheimer's disease(AD). The goal of this study was to explore their potential value as biomarkers for the diagnosis of AD. Methods The expression levels of exosomal miR-135 a,-193 b, and-384 in the serum from mild cognitive impairment(MCI), dementia of Alzheimer-type(DAT), Parkinson's disease with dementia(PDD), and vascular dementia(VaD) patients were measured with a real-time quantitative reverse transcriptase PCR(qR T-PCR) method. Results Both serum exosome mi R-135 a and miR-384 were up-regulated while miR-193 b was down-regulated in serum of AD patients compared with that of normal controls. Exosome miR-384 was the best among the three miR s to discriminate AD, VaD, and PDD. Using the cut-off value could better interpret these laboratory test results than reference intervals in the AD diagnosis. ROC curve showed that the combination of mi R-135 a,-193 b, and-384 was proved to be better than a particular one for early AD diagnosis. Conclusion Our results indicated that the exosomal miR s in the serum were not only potential biomarker of AD early diagnosis, but might also provide novel insights into the screen and prevention of the disease. | YANG Ting Ting LIU Chen Geng GAO Shi Chao ZHANG Yi WANG Pei Chang | 2018 | Biomedical and Environmental Sciences2018，31，2： | 35 |
| 3 | Phase I trial of icotinib, a novel epidermal growth factor receptor tyrosine kinase inhibitor, in Chinese patients with non-small cell lung cancer显示文摘 | WANG Hart-ping ZHANG Li WANG Yin-xiang TAN Fen-lai XIA Ying REN Guan-jun HU Pei JIANG Ji WANG Meng-zhao XIAO Yi | 2011 | Chinese Medical Journal2011，，13： | 23 |
| 4 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 5 | Tumor suppressor miR-128-3p inhibits metastasis and epithelial-mesenchymal transition by targeting ZEB1 in esophageal squamous-cell cancer显示文摘MicroRNAs (miRNAs ) 是在 post-transcriptional 层次调整多重生物功能的一些短 RNA,例如 tumorigenic 过程,煽动性的损害和房间 apoptosis。(ZEB1 ) 锌手指电子盒子有约束力的 homeobox 因素 1 是上皮间充质的转变(EMT ) 的一个关键调停人。它包括人的食道的有鳞房间的癌(ESCC ) 导致各种各样的癌症的恶意的前进。在这研究,我们发现 miR-128-3p 由使用 PCR 是在 ESCC 纸巾和房间的 downregulated。而且, miR-128-3p 的下面调整的表示被证明与 ESCC 病人的差的预后被联系并且可能被认为是一个独立预示的因素。然后,我们在 ESCC 房间检验了 miR-128-3p 的角色,并且发现 miR-128-3p 能在 vitro 压制房间迁居和侵略。而且, ZEB1 被证实是由酶记者试金的 miR-128-3p 的一个直接目标。营救实验证明 EMT 被 miR-128-3p 经由 ZEB1 的抑制调整。一起拿所有,我们断定 miR-128-3p 压制 EMT,经由 ZEB1 的转移,和 miR-128-3p 可以是在 ESCC 的一个批评调停人。 | Lili Zhao Rui Li Shanling Xu Yi Li Pei Zhao Wei Dong Zhenjun Liu Qian Zhao Bo Tan | 2018 | Acta Biochimica et Biophysica Sinica2018，50，2： | 21 |
| 6 | Drug-resistant gene based genotyping for Acinetobacter baumannii in tracing epidemiological events and for clinical treatment within nosocomial settings显示文摘背景 Acinetobacter baumannfi 在全世界作为与严重感染和医院的爆发有关的重要病原体出现了。经常使用的方法,然而,脉搏地胶化电气泳动(PFGE ) 和在 Acinetobacter baumannii genotyping 的放大碎片长度多型性(AFLP ) 缺乏药抵抗的直接分子的证明。这研究被进行基于药建立一个打字方法与在从2005年1月的医院的流行病或到2005年10月的 outbreak.Methods 的经期的传统的 PFGE 和 AFLP 相对照的抵抗基因鉴定,来自特别护理单位的 Acinetobacter 种类的 27 紧张,在 Ningbo 的第二所隶属于的医院被孤立,包括流行、分散的事件。危险性测试, PFGE, AFLP 和打字的药抵抗基因(DRGT ) 在外面是 carded 证实药抵抗并且分别地分析 genotyping。PFGE 被用作一本参考书评估 Acinetobacter 的 27 紧张显示了的 DRGT 和 AFLP.Results 的 typeability 多重抗菌素抵抗和药抵抗基因,和 -lactamase 基因在 85.2% 紧张被检测。尽管小差别存在, DRGT 的结果比得上在有不同的药抵抗的 Acinetobacter 紧张的 PFGE,并且甚至建议了不同药抵抗的紧张的一堂分子的进化功课。AFLP 显示出在紧张之间的大多型性并且在区分比作 AFLP 和 PFGE 的药 resistance.Conclusion 有弱能力, DRGT 是有用的分析医院的感染和爆发的局部性的分子的传染病学,它将有益于临床的诊断和治疗。 | JIN Hui XU Xiao-min MI Zu-huang MOU Yi LIU Pei | 2009 | Chinese Medical Journal2009，，3： | 18 |
| 7 | Effects of Honghua preserved amniotic membrane on scar healing in experimental glaucoma surgery显示文摘AIM:To investigate the efficacy and safety of Honghua preserved amniotic membrane(AM)for preventing scar formation of the filtering bleb in a rabbit model of glaucoma trabeculectomy surgery.METHODS:Totally 36 rabbits(36 eyes)were randomly divided into 3 groups:the experimental group(ocular trabeculectomy in combination with Honghua preserved AM transplantation),the control group(ocular trabeculectomy surgery in combination with AM implantation),and the blank group(single trabeculectomy).Clinical observations[including intraocular pressure(IOP),filtering blebs and complications],MassonTrichrome staining,real-time quantitative reverse transcription-polymerase chain reaction(real-time PCR),Western blot were performed on different time points(D1,D7,D14,D21 and D56)after the surgery.RESULTS:After operated for 14d,there were statistically significant differences in the filtering blebs compared to the situation before operation(P<0.05),whereas no statistically difference on that among three groups(P>0.05).After 21d,the IOP of experimental group was lowest(P<0.05).There was significant difference between control group and blank group(P<0.05).On postoperative D14,the mean number of fibroblasts in the experimental group was significantlylower(40.6±10.2)compared to those in the control group(54.4±10.8)and blank group(68.2±11.6)(P<0.05,respectively).The mean numbers of the macrophage in the experimental and control groups were respcitively significantly lower versus the blank group(P<0.05,P<0.05,respectively).Compared to that in blank group,the level of transforming growth factor-β(TGF-β1)expression in sclera and conjunctival areas was reduced in the experimental and control groups on protein and mRNA level(P<0.05),but not significant difference between these two groups(P>0.05).CONCLUSION:The trabeculectory surgery with Honghua preserved AM can control IOP,sustain the functional filtration bleb,inhibit the proliferation of fibroblasts and open the filtrating pathway on the rabbit glaucoma models. | Yi Shao Yao Yu Qiu-Ping Liu Jing-Ming Li Fei Dong Xin Huang Chong-Gang Pei Ping Tu Hen-Hui Li Gui-Ping Gao | 2014 | International Journal of Ophthalmology(English edition)2014，7，2： | 16 |
| 8 | Evaluation of novel decellularizing corneal stroma for cornea tissue engineering applications显示文摘·AIM:To develop a new decellularization method depended upon the natural corneal structure and to harvest an ideal scaffold with good biocompatibilities for corneal reconstruction.METHODS:The acellular cornea matrix (ACM) were prepared from de-epithelium fresh porcine corneas (DFPCs) by incubation with 100% fresh human sera and additional electrophoresis at 4℃. Human corneal epithelial cells (HCEs) were used for the cytotoxicity tests of ACM. ACM were implanted into the Enhanced Green Fluorecence Protein (eGFP) transgenic mouse anterior chamber for evaluation of histocompatibility.RESULTS:HE and GSIB4 results showed fresh porcine cornea matrix with 100% human sera and electrophoresis could entirely decellularize stromal cell without reducing its transparency. ACM has no cytotoxic effect ex vivo. Animal test showed there was no rejection for one month after surgery.CONCLUSION:These results provide a decellularizing approach for the study of corneal tissue engineering and had the broader implications for the field of biological tissue engineering in other engineered organ or tissue matrix. | Yi Shao, Lu Yang | 2012 | International Journal of Ophthalmology(English edition)2012，5，4： | 16 |
| 9 | Evapotranspiration and its main controlling mechanism over the desert riparian forests in the lower Tarim River Basin显示文摘Evapotranspiration(ET) and its controlling mechanism over the desert riparian forests in arid regions are the important scientific basis for the water resources managements of the lower reaches of the inland rivers of China. Nearly three years of continuous measurements of surface ET, soil water content at different depths and groundwater table over a typical Tamarix spp. stand and a typical Populus euphratica stand were conducted in the lower reach of the Tarim River. The ET seasonal trends in the growing season were controlled by plant phenology, and ET in non-growing season was weak. The diurnal variations of ET resulting from the comprehensive effects of all atmospheric factors were significantly related with reference ET. The spatial pattern of ET was determined by vegetation LAI, more vegetation coverage, more ET amount. Groundwater is the water source of surface ET, and the soil water in shallow layers hardly took part in the water exchange in the groundwatersoil-plant-air system. The temporal processes of ET over the Tamarix stand and the Populus stand were similar, but the water consumption of the well-grown Populus euphratica was higher than that of the well-grown Tamarix spp. Further analysis indicates that plant transpiration accounts for most of the surface ET, with soil evaporation weak and negligible; groundwater table is a crucial factor influencing ET over the desert riparian forests, groundwater influences the processes and amounts of ET by controlling the growth and spatial distribution of desert riparian forests; quantifying the water stress of desert riparian forests using groundwater table is more appropriate, rather than soil water content. Based on the understanding of ET and water movements in the groundwater-soil-plant-air system, a generalized framework expressing the water cycling and its key controlling mechanism in the lower reaches of the inland rivers of China is described, and a simple model to estimate water requirements of the desert riparian forests is presented. | YUAN GuoFu LUO Yi SHAO MingAn ZHANG Pei ZHU XuChao | 2015 | Science China Earth Sciences2015，58，6： | 14 |
| 10 | Effects of viral infection and microbial diversity on patients with sepsis:A retrospective study based on metagenomic next-generation sequencing显示文摘BACKGROUND: The study aims to investigate the performance of a metagenomic next-generationsequencing (NGS)-based diagnostic technique for the identifi cation of potential bacterial and viral infectionsand eff ects of concomitant viral infection on the survival rate of intensive care unit (ICU) sepsis patients.METHODS: A total of 74 ICU patients with sepsis who were admitted to our institution from February1, 2018 to June 30, 2019 were enrolled. Separate blood samples were collected from patients for bloodcultures and metagenomic NGS when the patients’ body temperature was higher than 38 °C. Patients’demographic data, including gender, age, ICU duration, ICU scores, and laboratory results, were recorded.The correlations between pathogen types and sepsis severity and survival rate were evaluated.RESULTS: NGS produced higher positive results (105 of 118;88.98%) than blood cultures(18 of 118;15.25%) over the whole study period. Concomitant viral infection correlated closelywith sepsis severity and had the negative effect on the survival of patients with sepsis. However,correlation analysis indicated that the bacterial variety did not correlate with the severity of sepsis.CONCLUSIONS: Concurrent viral load correlates closely with the severity of sepsis and thesurvival rate of the ICU sepsis patients. This suggests that prophylactic administration of antiviraldrugs combined with antibiotics may be benefi cial to ICU sepsis patients. | Li-wei Duan Jin-long Qu Jian Wan Yong-hua Xu Yi Shan Li-xue Wu Jin-hao Zheng Wei-wei Jiang Qi-tong Chen Yan Zhu Jian Zhou Wen-bo Yu Lei Pei Xi Song Wen-fang Li Zhao-fen Lin | 2021 | World Journal of Emergency Medicine2021，12，1： | 14 |
| 11 | COVID-19: Challenges to GIS with Big Data显示文摘The outbreak of the 2019 novel coronavirus disease(COVID-19)has caused more than 100,000 people infected and thousands of deaths.Currently,the number of infections and deaths is still increasing rapidly.COVID-19 seriously threatens human health,production,life,social functioning and international relations.In the fight against COVID-19,Geographic Information Systems(GIS)and big data technologies have played an important role in many aspects,including the rapid aggregation of multi-source big data,rapid visualization of epidemic information,spatial tracking of confirmed cases,prediction of regional transmission,spatial segmentation of the epidemic risk and prevention level,balancing and management of the supply and demand of material resources,and socialemotional guidance and panic elimination,which provided solid spatial information support for decision-making,measures formulation,and effectiveness assessment of COVID-19 prevention and control.GIS has developed and matured relatively quickly and has a complete technological route for data preparation,platform construction,model construction,and map production.However,for the struggle against the widespread epidemic,the main challenge is finding strategies to adjust traditional technical methods and improve speed and accuracy of information provision for social management.At the data level,in the era of big data,data no longer come mainly from the government but are gathered from more diverse enterprises.As a result,the use of GIS faces difficulties in data acquisition and the integration of heterogeneous data,which requires governments,businesses,and academic institutions to jointly promote the formulation of relevant policies.At the technical level,spatial analysis methods for big data are in the ascendancy.Currently and for a long time in the future,the development of GIS should be strengthened to form a data-driven system for rapid knowledge acquisition,which signifies ts that GIS should be used to reinforce the social operation parameterization of models and methods,especially when providing support for social management. | Chenghu Zhou Fenzhen Su Tao Pei An Zhang Yunyan Du Bin Luo Zhidong Cao Juanle Wang Wen Yuan Yunqiang Zhu Ci Song Jie Chen Jun Xu Fujia Li Ting Ma Lili Jiang Fengqin Yan Jiawei Yi Yunfeng Hu Yilan Liao Han Xiao | 2020 | Geography and Sustainability2020，1，1： | 13 |
| 12 | X-ray image characteristics and related measurements in the ankles of 118 adult patients with Kashin-Beck disease显示文摘 | Zeng Yi Zhou Zongke Shen Bin Yang Jing Kang Pengde Zhou Xuan Zou Ling Pei Fuxing | 2014 | Chinese Medical Journal2014，，13： | 13 |
| 13 | Nanoscale perspective: Materials designs and understandings in lithium metal anodes显示文摘 | Dingchang Lin Yayuan Liu Allen Pei Yi Cui | 2017 | Nano Research2017，10，12： | 12 |
| 14 | Bedaquiline-containing regimens in patients with pulmonary multidrug-resistant tuberculosis in China:focus on the safety显示文摘Background:World Health Organization recommends countries introducing new drug and short treatment regimen for drug resistant tuberculosis(DR-TB)should develop and implement a system for active pharmacovigilance that allows for detection,reporting and management of adverse events.The aim of the study is to evaluate the frequency and severity of adverse events(AEs)of bedaquiline-containing regimen in a cohort of Chinese patients with multidrug-resistant(MDR)/extensively drug-resistant(XDR)-TB based on active drug safety monitoring(aDSM)system of New Drug Introduction and Protection Program(NDIP). | Jing-Tao Gao Juan Du Gui-Hui Wu Yi Pei Meng-Qiu Gao Leonardo Martinez Lin Fan Wei Chen Li Xie Yu Chen Hua Wang Long Jin Guo-Bao Li Pei-Lan Zong Yu Xiong Qian-Hong Wu Ming-Wu Li Xiao-Feng Yan Yan-Fang Miao Qing-Shan Cai Xin-Jie Li Da-Peng Bai Shu-Jun Geng Guo-Li Yang Pei-JunTang Yi Zeng Xiao-Hong Chen Tong-Xia Li Cui Cai Yun Zhou Ma Zhuo Jian-Yun Wang Wen-Long Guan Lin Xu Ji-Chan Shi Wei Shu Li-Li Cheng FeiTeng Yu-Jia Ning Shi-Heng Xie Yu-Xian Sun Li-Jie Zhang Yu-Hong Liu | 2021 | Infectious Diseases of Poverty2021，10，2： | 11 |
| 15 | Clinical features and long-term prognosis of patients with anomalous origin of the left coronary artery from the pulmonary artery显示文摘从肺的动脉(ALCAPA ) 的左冠的动脉的背景异常起源是一个稀罕的先天的心异例。我们试图与 ALCAPA.Methods 说明临床的特征和病人的长期的预后 23 个病人( 13 男性和 10 女性,年龄从 2.5 个月 到 65 年)在北京 Anzhen 作为 ALCAPA 鉴别到2009年6月的从1984年4月的医院被划分成二个组,基于发作的年龄:组 1 (12 个月, n=16 ) 并且组 2 (12 个月, n=7 ) 56 削尖的 .Results 在组 1 的百分之三个病人作为 endocardial fibroelastosis (9/16 ) 被误诊, 18.8% 是扩大了心肌症(3/16 ) 和 6.3% 同样心肌的梗塞(1/16 ) 。在组 2 的病人通常作为冠的心疾病,心肌炎,或专利 ductus arteriosus 被诊断。在组 1 揭示反常 Q 的 Electrocardiography 处于领先与 T 波浪倒置飘动我, avL, V4-V6,特别在铅 avL (深、宽的 Q 波浪) ;但是在组 2 的没有特定的表明。在组 1 的病人的一个更高的百分比在胸拍上有心脏扩大症(86.7% 对 33.3% , P=0.031 ) 当肺的动脉伸出在组 2 是更普通的时(26.7% 对 83.3% , P=0.046 ) 。更低的左室的喷射部分(LVEF ) 在比在组 2 的组 1 是在场的((48.5 ? 桴 ? 慢吗? | ZHENG Jian-yong HAN Ling DING Wen-hong JIN Mei ZHANG Gui-zhen XIAO Yan-yan LUO Yi CHENG Pei MENG Xu ZHAO Qu-ming | 2010 | Chinese Medical Journal2010，，20： | 10 |
| 16 | Synthesis of a benzoxazine monomer containing maleimide and allyloxy groups显示文摘新奇 benzoxazine 单体 3-(4-allyloxy )phenyl-3,4-dihydro-2H-6-(N-maleimido )-1,3-benzoxazine (AMB ) 被综合,结构被英尺红外证实,(1 ) H NMR。AMB 的热分析(DSC ) 证明烯丙氧基组的介绍减少了熔点并且展出了一扇狭窄、对称的治好的发热的窗户。 | Yan Tang Qing Lai Zhao Ke Zeng Pei Kai Miao Ke Zhou Wen Rui Tang Hong Fei Zhou Tao Liu Yi Peng Wang Gang Yang | 2007 | Chinese Chemical Letters2007，18，8： | 10 |
| 17 | Modifi cation of wheat bran insoluble and soluble dietary fibers with snail enzyme显示文摘Insoluble dietary fiber(IDF)and soluble dietary fiber(SDF)extracted from wheat bran were modifi ed by snail enzyme and their physicochemical properties(water retention capacity and oil retention capacity),functional properties(cholesterol adsorption capacity,glucose adsorption capacity and antioxidant activity)and structural characterizations were evaluated.The results showed that snail enzyme modification led to the significant increase in oil retention capacity of IDF,glucose adsorption capacity and cholesterol adsorption capacity of IDF and SDF.Enzymatic modification also markedly improved the DPPH radical scavenging capacity and reducing power of IDF and SDF.Meanwhile,scanning electron microscopy(SEM)analysis indicated the microstructures of IDF and SDF powders were signifi cantly changed.Fourier transfer-infrared spectrometry(FT-IR)showed that snail enzyme modifi cation could degrade the part of cellulose and hemicellulose of IDF and SDF.All these improved physicochemical and functional properties of IDF and SDF might depend on their structural changes.It suggested that snail enzyme modifi cation could effectively improve physicochemical and functional properties of IDF and SDF from wheat bran. | Xin Liu Keke Suo Pei Wang Xue Li Limin Hao Jiaqing Zhu Juanjuan Yi Qiaozhen Kang Jinyong Huang Jike Lu | 2021 | Food Science and Human Wellness2021，10，3： | 10 |
| 18 | The Total Embedding Distributions of Cacti and Necklaces显示文摘全部的嵌入分布以仅仅图的一些班著名。在这份报纸,仙人掌和项链的全部的嵌入分布被获得。而且,我们获得与 1 由使用这的方法糊的最大的类嵌入所有图的分布的总数。关键词重叠矩阵 - 全部的嵌入分发 - 仙人掌,项链先生(2000 ) 题目分类 05C10 支持了由由不到资助号码 60373030 的 NNSFC 并且在资助号码 2004SM054 下面北京交通 Univeristy | Yi Chao CHEN Yah Pei LIU Tao WANG | 2006 | Acta Mathematica Sinica,English Series2006，22，5： | 9 |
| 19 | Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis显示文摘Objective To investigate the expression of miRNA-140 in chondrocytes and synovial fluid of osteoarthritis(OA) patients, and explore the relationship between the miRNA-140 expression and OA severity. Methods This study enrolled 30 OA patients who underwent total knee arthroplasty for chondrocytes sampling and 30 OA patients who underwent intra-articular injection for synovial fluid sampling. All OA patients were grouped into mild [Kellgren and Lawrence(KL) grade 1-2], moderate(KL grade 3) and severe(KL grade 4), with 10 in each subgroups for each sampling purposes. 7 non-OA patients and 10 patients with knee injury were collected for cartilage and synovial fluid sampling respectively as control groups. Chondrocytes were isolated from the cartilage tissue and cultured in vitro. Quantitative real time PCR for miRNA-140 in chondrocytes and synovial fluid were performed, and the U6 sn RNA was used as internal control. The expression difference of miRNA-140 among groups and correlation between the expression and the KL grade of OA were analysed using one-way ANOVA and Spearman test respectively. Results The expression of miRNA-140 in chondrocytes of knees in OA patients was reduced than that in normal knees, and the between-group difference was statistically significant(F=305.464, P<0.001). miRNA-140 could be detected in synovial fluid of both normal knees and OA knees, its relative expression level was reduced in synovial fluid of OA group compared with normal group, and the between-group difference was statistically significant as well(F=314.245, P<0.001). The relative expression level of miRNA-140 in both chondrocytes and synovial fluid were negatively correlated with the KL grade of OA(r=-0.969, P<0.001; r=-0.970, P<0.001). Conclusion miRNA-140 could be detected in chondrocytes and synovial fluid of OA patients, and its expression was negatively correlated with the severity of OA. | Hai-bo Si Yi Zeng Zong-ke Zhou Fu-xing Pei Yan-rong Lu Jing-qiu Cheng Bin Shen | 2016 | Chinese Medical Sciences Journal2016，31，4： | 9 |
| 20 | Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield显示文摘Artemisia annua is the major natural source of artemisinin,an anti-malarial medicine commonly used worldwide.Here,we present chromosome-level haploid maps for two A.annua strains with different artemisinin contents to explore the relationships between genomic organization and artemisinin production.High-fidelity sequencing,optical mapping,and chromatin conformation capture sequencing were used to assemble the heterogeneous and repetitive genome and resolve the haplotypes of A.annua.Approximately 5o,ooo genes were annotated for each haplotype genome,and a triplication event that occurred approximately 58.12million years ago was examined for the first time in this species.A total of 3,903,467-5,193,414 variants(SNPs,indels,and structural variants)were identified in the 1.5-Gb genome during pairwise comparison between haplotypes,consistent with the high heterozygosity of this species.Genomic analyses revealed a correlation between artemisinin concents and the copy number of amorpha-4,11-dienes ynthasegenes.This correlation was further confirmed by resequencing of 36A.annua samples with varied artemisinin contents.Circular consensus sequencing of transcripts facilitated the detection of paralog expression.Collectively,our study provides chromosome-level allele-aware genome assemblies for two A.annua strains and new insights into the biosynthesis of artemisinin and its regulation,which will contribute to conquering malaria worldwide. | Baosheng Liao Xiaofeng Shen Li Xiang Shuai Guo Shiyu Chen Ying Meng Yu Liang Dandan Ding Junqi Bai Dong Zhang Tomasz Czechowski Yi Li Hui Yao Tingyu Mai Caroline Howard Chao Sun Haitao Liu Jiushi Liu Jin Pei Jihai Gao Jigang Wang Xiaohui Qiu Zhihai Huang Hongyi Li Ling Yuan Jianhe Wei lan Graham Jiang Xu Boli Zhang Shilin Chen | 2022 | Molecular Plant2022，15，8： | 9 |
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