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| 1 | DNA microarray synthesis by using PDMS molecular stamps (Ⅲ)——Optimization for the reaction conditions显示文摘Optimization for the technological processes of fabricating oligonucleotide microarray by the molecular stamping method is studied in this note. Three factors that affect the pressing coupling reactions of the nucleosides are focused on: the stability of the chemical activities of the reaction solutions, the contamination of the remain of the reactive nucleotides among the different spots on the chip, and the influence of the capping reaction on the hybridization result. The experiments show that the acetonitrile solution of tetrazole and nucleoside monomer could maintain sufficient reactive activity for more than 10 h. An effective method has been used and proved to eliminate the residual reactive nucleosides on chip with small molecules containing hydroxylgroup. Finally, the capping step-a regular step in theconventional DNA chemical synthesis can be neglected in our on-chip DNA synthetic process, which would not affect its hybridization results. | XIAO Pengfeng, LU Zuhong, LIU Zhengchun, HE Quanguo & HE NongyueKey Laboratory for Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096, China | 2002 | Chinese Science Bulletin2002，47，13： | 5 |
| 2 | Current signal amplification strategies in aptamer-based electrochemical biosensor:A review显示文摘Due to their high specificity and affinity towards various targets,along with other unique advantages such as stability and low cost,aptamers are widely applied in analytical techniques.A typical aptamerbased electrochemical biosensor is composed of a aptamer as the biological recognition element and transducer converting the biologic interaction into electrical signals for the quantitative measurement of targets.Improvement of the sensitivity of a biosensor is significantly important in order to achieve the detection of biomolecules with low abundance,and different amplification strategies have been explored.The strategies either employ nanomaterials such as gold nanoparticles to con struct electrodes which can transfer the biological reactions more efficiently,or attempt to obtain enhanced signal through multi-labeled carriers or utilize enzyme mimics to catalyze redox cycling.This review discusses recent advances in signal amplification methods and their applications.Critical assessment of each method is also considered. | Lei He Rongrong Huang Pengfeng Xiao Yuan Liu Lian Jin Hongna Liu Song Li Yan Deng Zhu Chen Zhiyang Li Nongyue He | 2021 | Chinese Chemical Letters2021，32，5： | 3 |
| 3 | Feasibility of Isolation Remediation Technology for Heavy Metal Contaminated Soil显示文摘This paper discussed current situations of researches about the isolation remediation technology ' soil barrier and landfill technology' and ' physical isolation remediation technology' for heavy metal contaminated soil in mining areas.In view of defects of current technologies,it introduced a new isolation remediation technology,of which the new isolation materials were mixed by slaked lime,soil,find sand,and clay mineral in certain proportion.The new isolation remediation technology is expected to realize isolation remediation of heavy metal combined pollution of soil through chemical passivation of slaked lime and physical adsorption function of clay minerals or activated carbons. | Pengfeng LI Wei LIU Shuguang WANG | 2017 | Asian Agricultural Research2017，9，12： | 2 |
| 4 | Roles of Co element in Fe-based bulk metallic glasses utilizing industrial FeB alloy as raw material显示文摘A series of Fe-based bulk metallic glasses were fabricated by a conventional copper mold casting method using a kind of Fe-B industrial raw alloy. It is found that Fe-B-Y-Nb bulk metallic glass with 3 at% of Co addition possesses the best glass forming ability, thermal stability, hardness, magnetic property and anti-corrosion property. The hardness test result indicates a synchronically trend with glass-forming ability parameters. The excellent glass-forming ability and a combination of good mechanical and functional properties suggest that the alloys in this work might be good candidates for commercial use. | Shouyuan Wang Wei Jiang Haidong Hu Pengfeng Liu Jili Wu Bo Zhang | 2017 | Progress in Natural Science:Materials International2017，27，4： | 2 |
| 5 | The Solar Upper Transition Region Imager(SUTRI)Onboard the SATech-01 Satellite显示文摘The Solar Upper Transition Region Imager(SUTRI)onboard the Space Advanced Technology demonstration satellite(SATech-01),which was launched to a Sun-synchronous orbit at a height of~500 km in 2022 July,aims to test the on-orbit performance of our newly developed Sc/Si multi-layer reflecting mirror and the 2k×2k EUV CMOS imaging camera and to take full-disk solar images at the Ne VII 46.5 nm spectral line with a filter width of~3 nm.SUTRI employs a Ritchey-Chrétien optical system with an aperture of 18 cm.The on-orbit observations show that SUTRI images have a field of view of~416×416 and a moderate spatial resolution of~8″without an image stabilization system.The normal cadence of SUTRI images is 30 s and the solar observation time is about16 hr each day because the earth eclipse time accounts for about 1/3 of SATech-01's orbit period.Approximately15 GB data is acquired each day and made available online after processing.SUTRI images are valuable as the Ne VII 46.5 nm line is formed at a temperature regime of~0.5 MK in the solar atmosphere,which has rarely been sampled by existing solar imagers.SUTRI observations will establish connections between structures in the lower solar atmosphere and corona,and advance our understanding of various types of solar activity such as flares,filament eruptions,coronal jets and coronal mass ejections. | Xianyong Bai Hui Tian Yuanyong Deng Zhanshan Wang Jianfeng Yang Xiaofeng Zhang Yonghe Zhang Runze Qi Nange Wang Yang Gao Jun Yu Chunling He Zhengxiang Shen Lun Shen Song Guo Zhenyong Hou Kaifan Ji Xingzi Bi Wei Duan Xiao Yang Jiaben Lin Ziyao Hu Qian Song Zihao Yang Yajie Chen Weidong Qiao Wei Ge Fu Li Lei Jin Jiawei He Xiaobo Chen Xiaocheng Zhu Junwang He Qi Shi Liu Liu Jinsong Li Dongxiao Xu Rui Liu Taijie Li Zhenggong Feng Yamin Wang Chengcheng Fan Shuo Liu Sifan Guo Zheng Sun Yuchuan Wu Haiyu Li Qi Yang Yuyang Ye Weichen Gu Jiali Wu Zhe Zhang Yue Yu Zeyi Ye Pengfeng Sheng Yifan Wang Wenbin Li Qiushi Huang Zhong Zhang | 2023 | Research in Astronomy and Astrophysics2023，23，6： | 1 |
| 6 | Prepara- tion of hydrophilie poly ( dimethylsiloxane ) stamps by plasma- induced grafting显示文摘 | He Quanguo Liu Zhengchun Xiao Pengfeng | 2003 | Langrnuir2003，19，17： | 1 |
| 7 | Mitochondrion-processed TERC regulates senescence without affecting telomerase activities显示文摘Mitochondrial dysfunctions play major roles in ageing.How mitochondrial stresses invoke downstream responses and how specificity of the signaling is achieved, however, remains unclear. We have previously discovered that the RNA component of Telomerase TERC is imported into mitochondria, processed to a shorter form TERC-53, and then exported back to the cytosol. Cytosolic TERC-53 levels respond to mitochondrial functions, but have no direct effect on these functions, suggesting that cytosolic TERC-53 functions downstream of mitochondria as a signal of mitochondrial functions. Here, we show that cytosolic TERC-53 plays a regulatory role on cellular senescence and is involved in cognition decline in 10 months old mice, independent of its telomerase function. Manipulation of cytosolic TERC-53 levels affects cellular senescence and cognition decline in 10 months old mouse hippocampi without affecting telomerase activity, and most importantly, affects cellular senescence in terc^-/- cells. These findings uncover a senescence-related regulatory pathway with a non-coding RNA as the signal in mammals. | Qian Zheng Peipei Liu Ge Gao Jiapei Yuan Pengfeng Wang Jinliang Huang Leiming Xie Xinping Lu Fan Di Tanjun Tong Jun Chen Zhi Lu Jisong Guan Geng Wang | 2019 | Protein & Cell2019，10，9： | 0 |
| 8 | Design,Fabrication and Assembly of the Solar Upper Transition Region Imager(SUTRI)显示文摘The Solar Upper Transition Region Imager(SUTRI)focuses on the solar transition region to achieve dynamic imaging observation of the upper transition region.In this paper,we report the optical system design,mechanical design,ultrasmooth mirror manufacture and measurement,EUV multilayer film coating,prelaunch installation and calibration for the SUTRI payload at IPOE,Tongji University.Finally,the SUTRI carried by the SATech-01 satellite was successfully set to launch.All functions of this telescope were normal,and the observation results obtained in orbit were consistent with the design. | Zhanshan Wang Jun Yu Runze Qi Chunling He Zhengxiang Shen Li Jiang Weichen Gu Jiali Wu Zhe Zhang Pengfeng Sheng Yifan Wang Yue Yu Zeyi Ye JingJing Xia Yujie Xing Hongfei Jiao Bin Ma Qiushi Huang Zhong Zhang Xianyong Bai Yuanyong Deng Hui Tian Nange Wang Lun Shen Song Guo Lei Jing Xiaobo Chen Yang Gao Xiaocheng Zhu Xiaofeng Zhang Junwang He Qi Shi Shuo Liu Xingzi Bi | 2023 | Research in Astronomy and Astrophysics2023，23，9： | 0 |
| 9 | Defoliation in Flue-cured Tobacco and Its Reasons显示文摘The flue-cured tobacco G80 is not only one of the characteristic varieties in Changsha,but also indispensable raw materials for the series of Baisha cigarette. Defoliation during the late stage of growth seriously reduces the yield and quality of tobacco,and limits the popularization and application of G80. In this paper,in order to investigate the reasons for the defoliation of G80,the transverse section structure,cellulose content and differential proteins of leaf major veins were analyzed. The results revealed that the autolysis and collapse of parenchyma cells is the main cause of defoliation,and fungal infection is a factor inducing the autolysis of parenchyma cells. Application of bud suppression agent is one of the main ways to prevent fungal infection. The low cellulose content,low synthetic ability of xylan and pectin,the low removal ability of abnormal protein and low immune responsiveness are the inner reason for defoliation of flue-cured tobacco G80. | Chungui TANG Fan LI Hongwu YANG Pengfeng CHEN Li LI Jiamei LI Xinjian CHEN Hongxiang GUO Weiqun LIU | 2017 | Agricultural Biotechnology2017，6，3： | 0 |
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