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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | The Association of Maternal Body Composition and Dietary Intake with the Risk of Gestational Diabetes Mellitus during the Second Trimester in a Cohort of Chinese Pregnant Women显示文摘Objective To investigate the association of maternal body composition and dietary intake with the risk of gestational diabetes mellitus(GDM). Methods A total 154 GDM subjects and 981 controls were enrolled in a prospective cohort study in 11 hospitals from May 20, 2012 to December 31, 2013. Bioelectrical impedance analysis and dietary surveys were used to determine body composition and to evaluate the intake of nutrients in subjects at 21-24 weeks' gestation(WG). Logistic regression analysis was applied to explore the relationships of maternal body composition and dietary intake with the risk of GDM morbidity. Results Age, pre-pregnant body weight(BW), and body mass index(BMI) were associated with increased risk of GDM. Fat mass(FM), fat mass percentage(FMP), extracellular water(ECW), BMI, BW, energy, protein, fat, and carbohydrates at 21-24 WG were associated with an increased risk of GDM. In contrast, fat free mass(FFM), muscular mass(MM), and intracellular water(ICW) were associated with a decreased risk of GDM. Conclusion Maternal body composition and dietary intake during the second trimester of pregnancy were associated with the risk of GDM morbidity. | Xu Qing Gao Zhi Ying Li Li Ming Wang Lu Zhang Qian Teng Yue Zhao Xia Ge Sheng Jing Hong Jiang Yang Yong Tao Liu Xiao Jun Lyu Chun Jian Mao Lun Yu Xiao Ming Liu Ying Hua Kong Ai Jing Yang Xue Yan Liu Zhao Zhang Yong Wang Jin Zhang Xin Sheng Xue Chang Yong Lu Yan Ping | 2016 | Biomedical and Environmental Sciences2016，29，1： | 29 |
| 3 | Surveillance, epidemiology, and pathogen spectrum of hand, foot, and mouth disease in mainland of China from 2008 to 2017显示文摘Hand,foot and mouth disease(HFMD)was reported in May 2,2008 to be the 38th legally notifiable disease in China's National Notifiable Disease Reporting and Surveillance System.In order to solve the infection,an extensive three-level HFMD surveillance laboratory network was established.In this study,the framework of that network is assessed and the incidence of HFMD in China from 2008 to 2017 is reported using a descriptive epidemiologic method.During these 10 years,a series of techniques have been widely applied in all the network laboratories.Using information and material obtained from the network,a virus bank and database containing 18,238 viruses were established.Nationally,18,184,834 HFMD cases,including 152,436 severe cases and 3633 fatal cases,were reported in mainland of China.The average annual incidence in the population was 133.99/100,000 people,with a maximum incidence of 205.06/100,000 people in 2014.The incidence and mortality rates of HFMD were the highest in children aged 1–2 years.The numbers of reported cases fluctuated,with a high incidence observed every 2 years.An overall increase in the number of reported cases was also observed throughout the study period.Despite this,the incidence of severe cases and the mortality rate have been decreasing.High-risk regions are located in southern,eastern,and central China.Two peaks of HFMD infection cases were observed annually except for Northeast China.Different proportions of enterovirus serotypes were observed during the studied years.The predominant enterovirus varies from year to year,but the disease severity is always closely related to the specific serotype.EV-A71 is the dominant serotype associated with severe and fatal cases,with constituent ratios of 70.03%and 92.23%,respectively.The studied highly sensitive and efficient surveillance network provides information that is critical for prevention and control of the disease.It is extremely necessary and important to continuously conduct extensive virological surveillance for HFMD. | Tianjiao Ji Taoli Han Xiaojuan Tan Shuangli Zhu Dongmei Yan Qian Yang Yang Song Aili Cui Yan Zhang Naiying Mao Songtao Xu Zhen Zhu Dandan Niu Yong Zhang Wenbo Xu | 2019 | Biosafety and Health2019，1，1： | 26 |
| 4 | Diagnostic efficiency and complication rate of CT-guided lung biopsy: a single center experience of the procedures conducted over a 10-year period显示文摘计算断层摄影术( CT )指导了的背景 transthoracic 肺活体检视是一种生长得很好的技术因为这研究客观的肺的 lesions.The 的诊断是在中国 population.Methods 评估诊断效率和 指导CT 的肺活体检视的复杂并发症率 指导CT 的切针肺活体检视在在1月之间的 1014 个病人上在我们的机构被执行 2000 并且2010年10月 .A 胸拍被像关于基本耐心的信息的 biopsy.Data ,最后的 diagn 仅仅 53 个病人没得到期末考试 biopsy.Final 诊断的从肺的诊断在 131 个病人发现 639 恶意的损害( 63.0 )和 322 良性的损害( 31.8 ) .Pneumothoraxoccurred ,脊间的 drain.Small hemoptysis 的 15 要求的插入在 41 个病人发生了,温和 parenchymal 出血处于 16 patients.The 外套复杂并发症率发生了肺的损害的 指导CT 的切针活体检视是的 18.5 .Conclusions 有高诊断的 accuracy.It | YUAN Dong-mei LU Yan-ling YAO Yan-wen LIU Hong-bing WANG Qian XIAO Xin-wu CAO E-hong SHI Yi ZHOU Xiao-jun t SONG Yong | 2011 | Chinese Medical Journal2011，，20： | 22 |
| 5 | Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro显示文摘AIM: To study the inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma (HCC) cells in vitro.METHODS: Antisense RNA of HAb18G/CD147 vector PCIasHAb18G was constructed by reversely inserting HAb18G/CD147 cDNA to eukaryotic expression vector PCI-neo. The HCC cell line HHCC was transfected by PCI-asHAb18G via cation liposome. Expression of HAb18G/CD147 of transfected cells selected by G418 (geneticin) was observed by immunohistochemical SP staining and FACS (fluorescence activated cell sorting). Gelatin zymography was used to determine the effect of PCI-asHAb18G on reducing secretions of MMP2 and MMP-9 of the transfected cells. Boyden chamber was employed to test the invasion of HCC cells in vitro.RESULTS: The construction of antisense RNA vector PCIasHAb18G was verified correct by partial nucleotide sequencing and restricted endonuclease digestion. The expression of HAb18G/CD147 in transfected HHCC was inhibited by PCI-asHAb18G. Secretions of MMP-2 and MMP9 of transfected HHCC were reduced and the invasion of transfected HHCC was inhibited compared to HHCC,respectively.CONCLUSION: Invasion of HCC cells can be inhibited by antisense RNA of HAb18G/CD147. HAb18G/CD147 may be used as a potential target of drugs for anti-invasion and metastasis of HCC. | Yu Li Peng Shang Ai-Rong Qian Li Wang Yong Yang Zhi-Nan Chen, Department of Cell Biology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China | 2003 | World Journal of Gastroenterology2003，9，10： | 22 |
| 6 | The Role of Fibrocyte in the Pathogenesis of Silicosis显示文摘Exposure to free silica induces silicosis and myofibroblasts are regarded as primary effector cells.Fibrocytes can differentiate into myofibroblast.Therefore,the present study was designed to investigate whether | LI Juan YAO Wu HOU Jian Yong ZHANG Lin BAO Lei CHEN Hui Ting WANG Di YUE Zhong Zheng LI Yi Ping ZHANG Miao YU Xing Hao ZHANG Jian Hui QU Ya Qian HAO Chang Fu | 2018 | Biomedical and Environmental Sciences2018，31，4： | 20 |
| 7 | Gambogic acid induces mitochondria-dependent apoptosis by modulation of Bcl-2 and Bax in mantle cell lymphoma JeKo-1 cells显示文摘Aim: To study the mechanisms in gambogic acid (GA) -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro. Methods: The proliferation of GA-treated JeKo-1 cells was measured by CCK-8 assay and Ki-67 immunocytochemical detection. Apoptosis, cell cycle and mitochondrial membrane potential were measured by flow cytometric analysis. Caspase-3,-8 and -9 were detected by colorimetric assay. Bcl-2 and Bax were analyzed by Western blotting. Results: GA inhibited cell growth in a time-and dose-dependent manner. GA induces apoptosis in JeKo-1 cells but not in normal bone marrow cells, which was involved in reducing the membrane potential of mitochondria, activating caspases-3, -8 and -9 and decreasing the ratio of Bcl-2 and Bax without cell cycle arresting. Conclusions: GA induced apoptosis in human MCL JeKo-1 cells by regulating Bcl-2/Bax and activating caspase-3, -8 and -9 via mitochondrial pathway without affecting cell cycle. | Jingyan Xu Min Zhou Jian Ouyang Jing Wang Qiguo Zhang Yong Xu Yueyi Xu Qian Zhang Xihui Xu Hui Zeng | 2013 | Chinese Journal of Cancer Research2013，25，2： | 18 |
| 8 | Ginsenoside Rk1 suppresses pro-inflammatory responses in lipopolysaccharide-stimulated RAW264.7 cells by inhibiting the Jak2/Stat3 pathway显示文摘The saponin ginsenoside Rk1 is a major compound isolated from ginseng.Ginsenoside Rk1 has been reported to have anti-inflammatory and anti-tumor properties and to be involved in the regulation of metabolism.However,the effect and mechanism of anti-inflammatory action of ginsenoside Rk1 has not been fully clarified.We investigated whether ginsenoside Rk1 could suppress the inflammatory response in lipopolysaccharide-stimulated RAW264.7 macrophages and to explore its mechanism of the action.RAW264.7 cells were treated with LPS(1 μg×mL^(–1))in the absence or the presence of Ginsenoside Rk1(10,20,and 40 μmol×L^(–1)).Then the inflammatory factors were tested with Griess reagents,ELISA,and RT-PCR.The proteins were analyzed by Western blotting.Ginsenoside Rk1 inhibited lipopolysaccharide-induced expression of nitric oxide(NO),interleukin(IL)-6,IL-1β,tumor necrosis factor(TNF)-α,and monocyte chemotactic protein(MCP)-1.Ginsenoside Rk1 inhibited the lipopolysaccharide-stimulated phosphorylation of NF-κB and janus kinase(Jak)2 and signal transducer and activator of transcription(Stat)3 at Ser727 and Tyr705.These data suggested that ginsenoside Rk1 could inhibit expression of inflammatory mediators and suppress inflammation further by blocking activation of NF-κB and the Jak2/Stat3 pathway in LPS-stimulated RAW264.7 cells. | YU Qian ZENG Ke-Wu MA Xiao-Li JIANG Yong TU Peng-Fei WANG Xue-Mei | 2017 | Chinese Journal of Natural Medicines2017，15，10： | 17 |
| 9 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 10 | Flame retardant polyamide 6 by in situ polymerization of ε-caprolactam in the presence of melamine derivatives显示文摘An improved method for preparing melamine cyanurate (MCA) based flame retardant polyamide 6 (FRPA6) materials has been proposed. This processing method, i.e., improved in situ polymerization, was used to synthesize flame retardant PA6. In situ formed MCA nanoparticles were supposed to be linked to PA6 chains in the e-caprolactam hydrolytic polymerization system to obtain star- type polymers for the first time. Through TEM photographs, it can be found that the in situ formed MCA nanoparticles with diametric size of less than 50 nm, are nanoscaled, highly uniformly dispersed in the PA6 matrix. Synthesized ?ame retardant PA6 have good fire performance which can achieve UL-94 V-0 rating at 1.6 mm thickness with the presence of 7.34 wt.% MCA in the matrix. | Zhi Yong Wu Wei Xu Jin Kui Xia Yao Chi Liu Qian Xin Wu Wei Jian Xu | 2008 | Chinese Chemical Letters2008，19，2： | 15 |
| 11 | Shanghai Score: A Prognostic and Adjuvant Treatment-evaluating System Constructed for Chinese Patients with Hepatocellular Carcinoma after Curative Resection显示文摘 | Hui-Chuan Sun Lu Xie Xin-Rong Yang Wei Li Jian Yu Xiao-Dong Zhu Yong Xia Ti Zhang Yang Xu Bo Hu Li-Ping Du Ling-Yao Zeng Jian Ouyang Wei Zhang Tian-Qiang Song Qiang Li Ying-Hong Shi Jian Zhou Shuang-Jian Qiu Qian Liu Yi-Xue Li Zhao-You Tang Yu Shyr Feng Shen Jia Fan | 2017 | Chinese Medical Journal2017，，22： | 15 |
| 12 | Rb1 protects endothelial cells from hydrogen peroxide-induced cell senescence by modulating redox status显示文摘Objectives Endothelial senescence has been proposed to be involved in endothelial dysfunction and atherogenesis. This study investigates the effects of ginsenoside Rbl, a major constituent of ginseng,on H2O2-induced endothelial senescence.Methods Primary human umbilical vein endothelial cells(HUVECs) senescence was induced by H2O2 as judged by senescence-associated P-galactosidase assay (SA-P-gal).Fntracellur superoxide dismutase(S0D1) activity and malondialdehyde(MDA) level were determined by commercial kit.S0D1 mRNA and protein expression were analyzed by real time PCR and Western blot.Reactive oxygen species(ROS) were determined by flow cytometry.Results Rb1 was found to reverse endothelial senescence,as witnessed by a significant decrease of senescent cell numbers. Rbl could markedly increase intracellular SOD activity, decrease the MDA level,and suppress the generation of intracellular ROS in H2O2-treated HUVECs.Consistent with these findings,Rbl can effectively restore SOD1 mRNA and protein expression which decreased in H2O2 treated cells. Conclusions Our report demonstrates thatRbl can exert reversal effects on H2O2-induced cellular senescence through modulating cellular redox status. | LIU Ding-hui~1,CHEN Yan-ming~2,LIU Yong~1,HAO Bao-shun~1, ZHOU Bin~1,WU Lin~1,WANG Min~1,CHEN Lin~1,WU Wei-kang~3,QIAN Xiao-xian~1 (1.Department of Cardiology,The Third Affiliated Hospital of Sun Yat-sen University,Guagnzhou 510630 2.Department of Endocrinology,The Third Affiliated Hospital of Sun Yat-sen, Guagnzhou 510630 3.Institute Integrated Traditional Chinese and Western Medicine,Sun Yat-sen University,Guagnzhou 510630) | 2011 | 岭南心血管病杂志2011，17，S1： | 15 |
| 13 | An economic tie network-structure analysis of urban agglomeration in the middle reaches of Changjiang River based on SNA显示文摘由于它在集成地区性的协调开发并且提高华中的上升的大战略意义,在 Changjiang (Yangtze ) 河的中间的活动范围的城市的凝块从理论、实际的方面吸引了许多注意。对区域经济网络结构的如此的研究为城市发展、地区性发展的策略的形成是有益的。这篇论文基于一个修改引力模型构造一个经济领带模型。随后,指社会网络分析,论文以经验为主地学习网络密度,网络中心性,亚群和 Changjiang 河城市的凝块的中间的活动范围的结构的洞经济网络。调查结果是四折的:(1 ) 在 Changjiang 河的中间的活动范围的城市的凝块的一个经济网络被形成了,并且在在这个网络的城市之间的经济关系是比较地稠密的;( 2 )在 Changjiang 河的中间的活动范围的城市的凝块能被划分成四重要亚群,与有它的自己的明显的经济通讯的每亚群,当在亚群之中有不太经济行为的异质时,这为在波伊昂·莱克存在的二亚群是特别真的生态的经济地区;( 3 )由武汉,长沙和南昌的中央城市驾驶的一个经济模式出现在 Changjiang 河的中间的活动范围的城市的凝块,当这三个首都的城市施加了大放射能力到他们的包围城市时,后者不太能从前者吸收资源;(4 ) 武汉大城市的区域和波伊昂·莱克生态的经济地区比长沙, Zhuzhou 和 Xiangtan 城市簇的戒指有更结构的洞,意味着在这二个区域的圆周的城市是容易由中央城市抑制了。长沙, Zhuzhou 和 Xiangtan 城市的戒指聚类有更少结构的洞;因此,在这个区域的城市不在另外的二个区域与那些比面对同样多限制。 | SUN Qian TANG Fanghua TANG Yong | 2015 | Journal of Geographical Sciences2015，25，6： | 15 |
| 14 | Preparation and Characterization of KNO3/Diatomite Shape-Stabilized Composite Phase Change Material for High Temperature Thermal Energy Storage显示文摘A new potassium nitrate(KNO_3)/diatomite shape-stabilized composite phase change material(SSCPCM) was prepared by the mixing and sintering method. KNO_3 served as the phase change material(PCM) for thermal energy storage, while diatomite acted as the carrier matrix to provide the structural strength and prevent the leakage of PCM. It was found that KNO_3 could be retained 65 wt% into pores and on surfaces of diatomite without the leakage of melted KNO_3 from the SS-CPCM. The calculated filling rate of molten KNO_3 that could enter into the disc-like shape pore of diatomite verified the scanning electronic microscopy images of SS-CPCM. X-ray diffraction and Fourier transform infrared spectroscopy results showed that no reaction occurred between KNO_3 and diatomite, performing good compatibility. According to the differential scanning calorimetry results, after 50 thermal cycles, the phase change temperatures for melting and freezing of SS-CPCM with 65 wt% KNO_3 were changed from 330.23 °C and 332.90 °C to330.11 °C and 332.84 °C and corresponding latent heats varied from 60.52 J/g and 47.30 J/g to 54.64 J/g and 41.25 J/g, respectively. The KNO_3/diatomite SS-CPCM may be considered as a potential storage media in solar power plants for thermal energy storage. | Yong Deng Jinhong Li Tingting Qian Weimin Guan Xiang Wang | 2017 | Journal of Materials Science & Technology2017，33，2： | 15 |
| 15 | Optimized nitrogen application methods to improve nitrogen use efficiency and nodule nitrogen fixation in a maize-soybean relay intercropping system显示文摘In China, the abuse of chemical nitrogen(N) fertilizer results in decreasing N use efficiency(NUE), wasting resources and causing serious environmental problems. Cereal-legume intercropping is widely used to enhance crop yield and improve resource use efficiency, especially in Southwest China. To optimize N utilization and increase grain yield, we conducted a two-year field experiment with single-factor randomized block designs of a maize-soybean intercropping system(IMS). Three N rates, NN(no nitrogen application), LN(lower N application: 270 kg N ha–1), and CN(conventional N application: 330 kg N ha–1), and three topdressing distances of LN(LND), e.g., 15 cm(LND1), 30 cm(LND2) and 45 cm(LND3) from maize rows were evaluated. At the beginning seed stage(R5), the leghemoglobin content and nitrogenase activity of LND3 were 1.86 mg plant–1 and 0.14 m L h–1 plant–1, and those of LND1 and LND2 were increased by 31.4 and 24.5%, 6.4 and 32.9% compared with LND3, respectively. The ureide content and N accumulation of soybean organs in LND1 and LND2 were higher than those of LND3. The N uptake, NUE and N agronomy efficiency(NAE) of IMS under CN were 308.3 kg ha–1, 28.5%, and 5.7 kg grain kg–1 N, respectively; however, those of LN were significantly increased by 12.4, 72.5, and 51.6% compared with CN, respectively. The total yield in LND1 and LND2 was increased by 12.3 and 8.3% compared with CN, respectively. Those results suggested that LN with distances of 15–30 cm from the topdressing strip to the maize row was optimal in maize-soybean intercropping. Lower N input with an optimized fertilization location for IMS increased N fixation and N use efficiency without decreasing grain yield. | YONG Tai-wen CHEN Ping DONG Qian DU Qing YANG Feng WANG Xiao-chun LIU Wei-guo YANG Wen-yu | 2018 | Journal of Integrative Agriculture2018，17，3： | 15 |
| 16 | Preliminary study on the production of transgenic mice harboring hepatitis B virus X gene显示文摘INTRODUCTIONDespiteoverwhelmingepidemiologicalevidencelinkingpersistentHepatitisBVirus(HBV)infectionandthedevelopmentofhepato... | ZHU Huan Zhang 1, CHENG Guo Xiang 2, CHEN Jian Qu 2, KUANG Shu Yuan 3, CHENG Yong 2, ZHANG Xin Li 1, Ll Hou Da 2, XU Shao Fu 2, SHI Jing Quan 1, QIAN Geng Sun 3 and GU Jian Ren 3 | 1998 | World Journal of Gastroenterology1998，4，6： | 14 |
| 17 | Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents,β-ionone and linalool显示文摘Sweet osmanthus(Osmanthus fragrans Lour.)is among the top ten most well-known flowers in China and is recognized as both an aromatic plant and ornamental flower.Here,manual sectioning,scanning electron microscopy,and transmission electron microscopy of sweet osmanthus petals revealed that large amounts of lipids are present inside the petal cells and on the cell surfaces.However,no secretory structures were observed.Instead,the petal cells protrude slightly outward,and the surfaces of the cells are adorned with highly regular brush-shaped hairs.The surfaces of the‘Yingui’petals possessed mostly curled and more numerous hairs,whereas the‘Dangui’petals possessed fewer brush-shaped and more sparsely arranged hairs.In addition,many granular substances were attached to the brush-shaped hairs,and the granules were denser on the hairs of the‘Yingui’petals compared to the hairs on the‘Dangui’petals.Furthermore,35 aromatic components in the‘Yingui’petals and 30 aromatic components in the‘Dangui’petals were detected via GC-MS.The main aromatic component of the‘Yingui’petals wasβ-ionone,whereas that of the‘Dangui’petals was linalool and its oxides.Transcriptome sequencing and qRT-PCR indicated that the highβ-ionone content in the‘Yingui’petals was due to the overexpression of CCD1 and CCD4 and that the high linalool content in the‘Dangui’petals was due to the overexpression of MECS,HDR,IDI1,and LIS1,which function upstream of the linalool synthetic pathway.In particular,the expression levels of CCD4 and LIS1 were upregulated by 5.5-and 5.1-fold in the‘Yingui’and‘Dangui’petals,respectively.One transcription factor(ERF61)was cloned and named,and the expression pattern of ERF61 in sweet osmanthus petals was found to be generally consistent with that of CCD4.Tobacco transformation experiments,yeast one-hybrid experiments,and electrophoretic mobility shift assays indicated that ERF61 binds to the CCD4 promoter and stimulates CCD4 expression,thereby regulating the synthesis ofβ-ionone in sweet osmanthus petals. | Yuanji Han Hongyun Wang Xiaodan Wang Ke Li Meifang Dong Yong Li Qian Zhu Fude Shang | 2019 | Horticulture Research2019，6，1： | 13 |
| 18 | Short-Impending Earthquake Anomaly Index Extraction of GNSS Continuous Observation Data in Yunnan,Southwestern China显示文摘This paper presents a comprehensive area expansion prediction index method to apply GNSS for short-impending prediction of earthquakes. Based on continuous GNSS observation data from Yunnan Province, a displacement field was detected after data cycle-slip repair using precision data processing software and geophysical field effect model correction. The Yunnan area was divided into 56 grid cells for displacement field interpolation to obtain a more uniform displacement field and a strain field variation time series. The pre-earthquake response of each grid-cell expansion time series was evaluated and synthesized to extract a short-impending earthquake anomaly identification index. The results show that this index indicated occurrence times and hypocenter for earthquakes of magnitude M≥5. Fourteen earthquakes were predicted accurately, and there were five false reports. This index can therefore be used for the short-impending prediction of earthquakes. | Min Hong Desheng Shao Tengfei Wu Shuangxi Zhang Yong Zhang Lingli Wang Xiaodong Qian | 2018 | Journal of Earth Science2018，29，1： | 13 |
| 19 | Chinese Medicine Sequential Therapy Improves Pregnancy Outcomes after Surgery for Endometriosis-Associated Infertility: A Multicenter Randomized Double-blind Placebo Parallel Controlled Clinical Trial显示文摘Objective:To evaluate the efficacy and safety of Chinese medicine(CM)improving pregnancy outcomes after surgery for endometriosis-associated infertility.Methods:A multicenter,randomized,doubleblind placebo parallel controlled clinical trial was designed.A total of 202 patients who had laparoscopy for endometriosis-associated infertility with qi stagnation and blood stasis syndrome were included and randomly divided into the CM treatment group and placebo control group at a ratio of 1:1 using a central block randomization from May 2014 to September 2017,101 patients in each group.The two groups received continuous intervention at 1–5 days after surgery,for 6 menstrual cycles.Before ovulation,the CM group was treated Huoxue Xiaoyi Granule(活血消异颗粒);after ovulation,Bushen Zhuyun Granule(补肾助孕颗粒)was involved.The control group was treated with placebo.Transvaginal ultrasonography was performed every menstrual cycle during the treatment,and female hormone levels in the follicular and luteal phases were measured during the 1 st,3 rd and 6 th menstrual cycles.The analysis was continued until pregnancy.The primary outcomes were clinical pregnancy rate and pregnancy outcome,and the secondary outcomes were follicular development and endometrial receptivity.Safety evaluations were performed before and after treatment.Results:(1)Clinical pregnancy and live birth rates:the clinical pregnancy and live birth rates of the CM group were significantly higher than those of the placebo group[44.6%(45/101)vs.29.7%(30/101),34.7%(35/101)vs.20.8%(21/101),both P<0.05].(2)Follicle development:the incidence of dominant follicles,rate of cumulative cycle ovulation,and rate of cumulative cycle mature follicle ovulation were significantly higher in the CM group than those in the placebo group[93.8%(350/373)vs.89.5%(341/381),80.4%(275/342)vs.69.1%(253/366),65.8%(181/275)vs 56.1%(142/253),P<0.05 or P<0.01]).The incidence of cumulative cycle luteinized unruptured follicle syndrome was significantly lower in the CM group than in the placebo group[11.7%(40/342)vs.17.8%(65/366),P<0.05).(3)Endometrial receptivity:after treatment,both endometrial types and endometrial blood flow types in the CM group were mainly types A and B,while those in the placebo group were mainly types B and C,with a significant difference between the two groups(both P<0.05).(4)Adverse events:the incidence of adverse events between the two groups was not significantly different(P>0.05).Conclusion:Strategies for activating blood circulation-regulating Gan(Liver)-tonifying Shen(Kidney)sequential therapy can effectively improve the clinical pregnancy rate and live birth rate of endometriosis-associated infertility with qi stagnation and blood stasis after laparoscopy,improve follicular development,promote ovulation,improve endometrial receptivity,while being a safe treatment option.(Trial registration No.NCT02676713). | ZHAO Rui-hua LIU Yong LU Dan WU Ying WANG Xiao-yun LI Wei-li ZENG Cheng MENG Qing-wei LIAN Feng-mei ZHOU Jun SHI Yun SUN Wei-wei HAN Qian TANG Yi SHI Guang | 2020 | Chinese Journal of Integrative Medicine2020，26，2： | 13 |
| 20 | PPARy agonists inhibit TGF-β-PKA signaling in glomeru Iosclerosis显示文摘 | Rong ZOU Gang XU Xiao-cheng LIU Min HAN Jing-jing JIANG Qian HUANG Yong HE Ying YAO | 2010 | Acta Pharmacologica Sinica2010，31，1： | 12 |
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