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| 1 | The CRISPR/Cas9 induces large genomic fragment deletions of MSTN and phenotypic changes in sheep显示文摘The CRISPR/Cas9 system has been extensively used to engineer genetic loci for the generation of knockouts, insertions, and point mutations in animal models. However, many mutations that have been reported in animals are small insertions or deletions. This study used the CRISPR/Cas9 system to induce large DNA fragment deletions in MSTN via three guide RNAs in sheep. This successfully achieved the precise gene editing of the ovine MSTN gene by injecting both Cas9 m RNA and sg RNAs into embryos at the one-cell stage. Of 10 edited animals, 3 animals(30%) exhibited large genomic fragment deletions(~5 kb). Furthermore, the body weights of these 3 animals were significantly different(P0<0.0001, P15=0.001, P30=0.005, P60=0.027) between lambs with large deletions and wildtype lambs. In addition, the edited lambs were also significantly different(P0<0.0001, P15<0.0001, P30=0.002, P60=0.011) compared with wildtype. These results suggest that the generated MSTN knockout sheep is a reliable and effective animal model for further study. Furthermore, this method is time-and labor-saving, and efficient for the creation of animal models for agriculture, biology, and medicine. | DING Yi ZHOU Shi-wei DING Qiang CAI Bei ZHAO Xiao-e ZHONG Shu JIN Miao-han WANG Xiao-long MA Bao-hua CHEN Yu-lin | 2020 | Journal of Integrative Agriculture2020，19，4： | 8 |
| 2 | Constraint-induced movement therapy promotes motor function recovery and downregulates phosphorylated extracellular regulated protein kinase expression in ischemic brain tissue of rats显示文摘Motor function impairment is a common outcome of stroke.Constraint-induced movement therapy(CIMT)involving intensive use of the impaired limb while restraining the unaffected limb is widely used to overcome the effects of‘learned non-use’and improve limb function after stroke.However,the underlying mechanism of CIMT remains unclear.In the present study,rats were randomly divided into a middle cerebral artery occlusion(model)group,a CIMT+model(CIMT)group,or a sham group.Restriction of the affected limb by plaster cast was performed in the CIMT and sham groups.Compared with the model group,CIMT significantly improved the forelimb functional performance in rats.By western blot assay,the expression of phosphorylated extracellular regulated protein kinase in the bilateral cortex and hippocampi of cerebral ischemic rats in the CIMT group was significantly lower than that in the model group,and was similar to sham group levels.These data suggest that functional recovery after CIMT may be related to decreased expression of phosphorylated extracellular regulated protein kinase in the bilateral cortex and hippocampi. | Bei Zhang Qiang He Ying-ying Li Ce Li Yu-long Bai Yong-shan Hu Feng Zhang | 2015 | Neural Regeneration Research2015，10，12： | 5 |
| 3 | Characterization of DNA damage response deficiency in pancreatic cancer patients from China显示文摘To the editor,Pancreatic ductal adenocarcinoma(PDAC)has the worst prognosis among all common malignant solid tumors,with a 5-year overall survival(OS)rate of less than 10%[1].Few effective targets for anticancer ther-apy have been confirmed in pancreatic cancer.Recently,it was substantiated that pancreatic cancer patients carry-ing deleterious mutations of the DNA damage response(DDR)genes are more likely to benefit from platinum-based chemotherapy[2]and poly(adenosine diphosphate-ribose)polymerase(PARP)inhibitor[3]. | Xiaofei Zhang Tiebo Mao Bei Zhang Haiyan Xu Jiujie Cui Feng Jiao Dongqin Chen Yu Wang Jiong Hu Qing Xia Shumin Li Ming Yue Jingyu Ma Jiayu Yao Yongchao Wang Xiao Zhang Shiqing Chen Yuezong Bai Yuexiang Wang Xuebin Zhang Qiang Liu Yongwei Sun Deliang Fu Yingbin Liu Lei Xiong Liwei Wang | 2022 | Cancer Communications2022，42，1： | 2 |
| 4 | HMGB1 induces secretion of matrix vesicles which participate in microcalcification of atherosclerotic plaques显示文摘AIM: Early calcification of atherosclerotic plaques are colocalized with macrophage and high mobility group box 1( HMGB1),a cytokine associated with biomineralizing process under physiological and pathological conditions. Our study aims to evaluate whether HMGB1 induces ectopic mineralization via promoting the secretion of matrix vesicles( MVs) from macrophages. METHODS: HMGB1 was added to the medium of macrophages,the secretion of MVs in the supernatant was tested by flow cytometry analysis. The mineral deposition in calcifying medium was detected by Alizarin Red staining and von Kossa staining. Transmission electron microscopy showed the formation of hydroxyapatite crystals in MVs. Then we subcutaneous injection into mice with MVs to induce regional mineralization. RESULTS: HMGB1 significantly promoted secretion of MVs from macrophages as raveled by flow cytometry analysis. TNAP activity,considered as a marker of MVs maturation,was higher in HMGB1-induced MVs compared to the control-MVs. HMGB1-MVs also led to mineral deposition in an in vitro MVs-collagen mineralization model. Subcutaneous injection into mice with MVs derived from HMGB1-treated cells showed a greater potential to initiate regional mineralization. Mechanistic experiments revealed that HMGB1 activated neutral sphingomyelinase 2( n SMase2) that involved the receptor for advanced glycation end products( RAGE) and p38MAPK( upstream of n SMase2). Inhibition of n SMase2 with GW4869 or p38 MAPK with SB-239063 prevented MVs secretion and mineral deposition. CONCLUSIONS: HMGB1 induces MVs secretion from macrophages at least in part,via the RAGE / p38 MAPK /n SMase2 signaling pathway. Our findings thus reveal a novel mechanism by which HMGB1 may participated in the early calcification of atherosclerotic plaques. | CHEN Qiang BEI Jun-jie LIU Chuan FENG Shi-bin ZHAO Wei-bo ZHOU Zhou YU Zheng-ping DU Xiao-jun HU Hou-yuan | 2016 | 中国病理生理杂志2016，32，8： | 2 |
| 5 | Encapsulation of lyophilized platelet-rich fibrin in alginate-hyaluronic acid hydrogel as a novel vascularized substitution for myocardial infarction显示文摘Cardiovascular diseases such as myocardial infarction(MI)are among the major causes of death worldwide.Although intramyocardial injection of hydrogels can effectively enhance the ventricular wall,this approach is limited because of its restriction to the poor vascularization in the infarcted myocardium.Here,we reported a new type of hydrogel composed of alginate(ALG)and hyaluronic acid(HA)with lyophilized platelet-rich fibrin(Ly-PRF)for releasing abundant growth factors to realize their respective functions.The results of in vitro studies demonstrated favorable mechanical property and release ability of ALG-HA with Ly-PRF.When injected into the infarcted myocardium,this composite hydrogel preserved heart function and the Ly-PRF within the hydrogel promoted angiogenesis and increased vascular density in both infarcted and border zone,which rescued the ischemic myocardium.These beneficial effects were also accompanied by macrophage polarization and regulation of myocardial fibrosis.Moreover,the autologous origin of Ly-PRF with ALG-HA hydrogel offers myriad advantages including safety profile,easiness to obtain and cost-effectiveness.Overall,this study demonstrated the versatile therapeutic effects of a novel composite hydrogel ALG-HA with Ly-PRF,which optimizes a promising vascularized substitution strategy for improving cardiac function after MI. | Bei Qian Qi Yang Mingliang Wang Shixing Huang Chenyu Jiang Hongpeng Shi Qiang Long Mi Zhou Qiang Zhao Xiaofeng Ye | 2022 | Bioactive Materials2022，7，1： | 2 |
| 6 | Clinical application of free/total PSA ratio in the diagnosis of prostate cancer in men over 50 years of age with total PSA levels of 2.0-25.0 ng ml^(−1) in Western China显示文摘The goal of this study was to investigate the clinical application of free/total prostate-specific antigen(F/T PSA)ratio,considering the new broad serum total PSA(T-PSA)“gray zone”of 2.0–25.0 ng ml^(−1)in differential diagnosis of prostate cancer(PCa)and benign prostate diseases(BPD)in men over 50 years in Western China.A total of 1655 patients were included,528 with PCa and 1127 with BPD.Serum T-PSA,free PSA(F-PSA),and F/T PSA ratio were analyzed.Receiver operating characteristic curves were used to assess the efficiency of PSA and F/T PSA ratio.There were 47.4%of cancer patients with T-PSA of 2.0–25.0 ng ml^(−1).When T-PSA was 2.0–4.0 ng ml^(−1),4.0–10.0 ng ml^(−1),and 10.0–25.0 ng ml^(−1),the area under the curve(AUC)of F/T PSA ratio was 0.749,0.769,and 0.761,respectively.The best AUC of F/T PSA ratio was 0.811 when T-PSA was 2.0–25.0 ng ml^(−1),with a specificity of 0.732,a sensitivity of 0.788,and an optimal cutoff value of 15.5%.The AUC of F/T PSA ratio in different age groups(50–59 years,60–69 years,70–79 years,and≥80 years)was 0.767,0.806,0.815,and 0.833,respectively,and the best sensitivity(0.857)and specificity(0.802)were observed in patients over 80 years.The T-PSA trend was in accordance with the Gleason score,tumor node metastasis(TNM)stage,and American Joint Committee on Cancer prognosis group.Therefore,the F/T PSA ratio can facilitate the differential diagnosis of PCa and BPD in the broad T-PSA“gray zone”.Serum T-PSA can be a Gleason score and prognostic indicator. | Xue-Dan Gao Qiang Miao Jun-Long Zhang Jian-Zhao Zhai Xue-Mei Gui Yi-Han Cai Qian Niu Bei Cai | 2022 | Asian Journal of Andrology2022，24，2： | 2 |
| 7 | Signal transducer and activator of transcription 3 signaling upregulatesfascin via nuclear factor?κB in gastric cancer: Implications in cell invasionand migration显示文摘 | Jun Yao Cui?Juan Qian Bei Ye Zhi?Qiang Zhao Jie Wei Yong Liang Xin Zhang | 2014 | Oncology Letters2014，，3： | 1 |
| 8 | Trends and Regional Distribution Pattern of Female Lung Cancer--China,1992-2016显示文摘What is already known about this topic?The incidence of female lung cancer in China has been rising,whereas the incidence ratio among men and women is declining.The rising trend of female lung cancer is a prominent public health concern for China.What is added by this report?The joint secular trends distribution of the incidence,mortality,and years of life lost(YLLs)due to premature death of lung cancer in Chinese women demonstrated a certain regionality,which implied the risk factors for female lung cancer in China might be different by region.Common determinant risk factors may exist in regions sharing the same joint secular trends.What are the implications for public health practice?The secular trends and the regional pattern of the trends of female lung cancer in China suggested that the prevention and control of female lung cancer should be implemented with targeted regional interventions. | Xue Ni Bei Zhang Ning Xu Xinying Zeng Qiang Wang | 2020 | China CDC weekly2020，2，32： | 1 |
| 9 | Functional metagenomic characterization of antibiotic resistance genes in agricultural soils from China显示文摘 | Jian Qiang Su Bei Wei Chun Yan Xu Min Qiao Yong Guan Zhu | 2013 | Environment International2013，，： | 1 |
| 10 | A study of laparoscopic extraperitoneal sigmoid colostomy after abdomino-perineal resection for rectal cancer显示文摘Objective:To established a procedure for laparoscopic extraperitoneal ostomy after abdomino-perineal resection(APR)and study safety aspects and complications.Method:From July 2011 to July 2012,36 patients with low rectal cancer undergoing APR were included in the study and divided into extraperitoneal ostomy group(n=18)and intraperitoneal ostomy group(n=18).Short-and long-term complications were compared between the two groups.All patients were followed up and the median duration was 17 months(range:12-24).Results:The rates of short-term complication related to colostomies were comparable between the two groups,except the rate for stoma edema was higher in the extraperitoneal group(33.3%vs 0%;P=0.008).In the intraperitoneal ostomy group,two patients developed stoma prolapse,one had stoma stenosis,and two had parastomal hernia.In contrast,no long-term complications related to colostomies occurred in the extraperitoneal ostomy group.The rate of long-term complication was lower in the extraperitoneal ostomy group(0%vs 22.2%;P=0.036).Conclusion:The laparoscopic extraperitoneal ostomy is a relatively simple and safe procedure,with fewer long-term complications related to colostomy.However the follow-up period was not too long and needs to be extended. | Jin Heiying Du Yonghong Wang Xiaofeng Yao Hang Wu Kunlan Zhang Bei Zhang Jinhao Leng Qiang | 2014 | Gastroenterology Report2014，2，1： | 1 |
| 11 | Identification and validation of Temozolomide resistance hub gene LncRNA MOCS2-DT in glioblastoma显示文摘Background:Temozolomide(TMZ)is an important adjuvant chemotherapy drug after glioma surgery.However,with the progress of treatment,most patients will gradually develop drug resistance.Long non-coding RNA(lncRNA)in the prediction and reversal of chemotherapy drug resistance possesses great potential.Methods:1.The clinical data and RNA expression matrix of glioma patients were downloaded from the Chinese Glioma Genome Atlas(CGGA),and the clinical data and RNA expression profiles of 47 patients who only received TMZ chemotherapy after surgery were selected;2.WGCNA was performed on RNA expression profiles to find modules highly correlated with survival time and lncRNAs with high connectivity in modules.Core lncRNA were further screened from the GEO database(P<0.05);survival analysis,correlation analysis,enrichment analysis and co-expression analysis were performed;and 3.A TMZ concentration gradient induced glioma-resistant cells(U251TR).Cell proliferation toxicity assays and real-time quantitative PCR were used to verify the core lncRNA and identify the biological characteristics of U251TR.Results:1.According to the WGCNA analysis,it was found that the pink module is highly correlated with survival time and that there are 17 core lncRNAs with high connectivity in the pink module.2.The expression of the core lncRNA MOCS2-DT in the U251 resistant strain was significantly lower than that in the sensitive strain(P<0.05).3.U251TR cells had significantly larger cell bodies with irregular shapes.U251TR cells had a stronger tolerance to TMZ than U251 cells,and the resistance index was about four times higher(P<0.05).4.The expression levels of MGMT and ABCG2 in U251TR cells were significantly higher than those in parental U251 cells(P<0.05),and the expression levels of MOCS2-DT in U251TR cells were significantly lower than those in parental U251 cells(P<0.05).5.The survival time and survival rate of patients with low expression of MOCS2-DT were significantly lower than those of patients with high expression of MOCS2-DT in patients who only received TMZ chemotherapy after surgery.6.The enrichment analysis results show that the mRNA co-expressed with MOCS2-DT is enriched in synapse organization,regulation of membrane potential and signal release,indicating that MOCS2-DT is closely related to these functions.Conclusion:Targeted regulation of MOCS2-DT may reverse the drug resistance of glioma,improve the prognosis of patients and prolong the survival time of patients. | Lang Li Qiang Gu Jia-Hao Yao Bei Zhou Fa-Yan Dong Xue-Feng Wang | 2023 | Medical Theory and Hypothesis2023，6，2： | 0 |
| 12 | Translational oncology toward benefiting cancer patients:the Sun Yat-sen University Cancer Center experience显示文摘Cancer is a leading cause of death in China with an estimation of nearly 2 million deaths every year(Chen and Fu,2011b).Matter of a public health importance in China and worldwide,the scientific community is still facing | Mathilde Guerin Chaonan Qian Qian Zhong Qian Cui Yunmiao Guo Jinxin Bei Jianyong Shao Xiaofeng Zhu Wenlin Huang Jiangxue Wu Ranyi Liu Qiang Liu Jing Wang Weihua Jia Xiaohui Zheng Yixin Zeng | 2016 | Science China(Life Sciences)2016，59，10： | 0 |
| 13 | One-pot and One-step Cu(0)-mediated Reversible-Deactivation Radical Polymerization of N-Isopropylacrylamide(NIPAM) in Water显示文摘Copper(0)-mediated reversible-deactivation radical polymerization(Cu(0)-mediated RDRP) of the water-soluble monomer Nisopropylacrylamide(NIPAM) has been challenging with the problems of high dispersity, poor control over the molecular weights(MWs) or complex or multi reaction steps, etc. In this work, we report the well-controlled polymerization of NIPAM in water via a facile one-pot and one-step Cu(0)-mediated RDRP. The results of this approach show that the key for kicking off the Cu(0)-mediated NIPAM RDRPs is to ensure sufficient Cu~I at the very beginning, and the key to achieve a well-controlled chain growth is to provide adequate deactivation strength during the polymerization process. For NIPAM, which has a high propagation rate constant, the deactivation control can be effectively enhanced by extra adding deactivator(i.e., Cu~II) to the system. Moreover, a low reaction temperature(4 ℃) is necessary in the controlled synthesis of higher MW poly(Nisopropylacrylamide)(PNIPAM) to avoid the compromise in control caused by the phase transition from its lower critical solution temperature(LCST). Through this new kinetically controlled strategy, PNIPAMs with well-defined structure, narrow molecular weight distributions(MWDs) and varied MWs were successfully achieved. | Zi-Shan Li Jing Lyu Bei Qiu Ying-Hao Li Ruth Foley Ri-Jian Song Melissa Johnson Qiang Geng Wen-Xin Wang | 2024 | Chinese Journal of Polymer Science2024，42，1： | 0 |
| 14 | A Spatial Supply-Demand Imbalance Between Rural Primary and Secondary Schools in Shaanxi Province Based on Road Network Analysis显示文摘It is of great significance to optimize the spatial matching of basic education facilities in rural areas and promote the equalization of urban and rural basic public services and the overall revitalization of rural areas.Based on the theory of spatial accessibility and spatial balance of supply and demand,this paper constructs the evaluation system of rural basic education facilities and layout rationality.In this study,we took 42 primary and secondary schools and 2089 rural areas with concentrated population in six township areas of Hancheng City as the research subjects.We used ArcGIS network analysis,population coupling model,and other methods,from the administrative scale and rural population concentration area scale for evaluation.The accessibility of basic education facilities and its coupling relationship with the spatial distribution of student population describe the spatial and geographical pattern of basic education facilities in rural areas.The results show that the accessibility of rural administrative scale and the spatial balance of supply and demand of population concentration area scale are associated with great problems,the differences between towns are obvious,and the overall level of the best performing towns is still low.On the scale of population concentration area,the overall coupling degree is too low,the'dislocation'distribution of educational facilities and student population is obvious,and the matching degree of the student population and school in different towns is significantly different,showing a more obvious'core-periphery'spatial structure characteristics centered on the eastern and southeast regions.The terrain characteristics of rural areas in Shaanxi province are prominent,and the accessibility of the road network is particularly significant.Therefore,optimizing the construction of rural roads and optimizing school layout according to local conditions will become an important solution to promote the balanced allocation of basic education resources in Shaanxi province. | Ziwei Qiang Bei Jin Shiyuan Xu | 2022 | Journal of Contemporary Educational Research2022，6，2： | 0 |
| 15 | Benzothiadiazole-based materials for organic solar cells显示文摘The past few years have witnessed power conversion efficiency(PCE)of organic solar cells(OSCs)skyrocketing to the value of 20%due to the outstanding advantages of organic photoactive materials.The latter,which consist of donor and acceptor materials,indeed play important roles in OSCs,and particularly one building block has attracted considerable research attention,namely benzothiadiazole(BT).The diversity of OSCs based on the BT structure have indeed sprung up,and the progressive increase in PCE values is more than just eye-catching since it heralds a renewal and bright future of OSCs.This review analyzes significant studies that have led to these remarkable progresses and focuses on the most effective BT small-molecules and BT polymers for OSC reported in the last decades.The pivotal structure-property relationships,donor-acceptor matching criteria,and morphology control approaches are gathered and discussed in this paper.Lastly,we summarize the remaining challenges and offer a personal perspective on the future advance and improvement of OSCs. | Qiang Bei Bei Zhang Kaifeng Wang Shiming Zhang Guichuan Xing Clément Cabanetos | 2024 | Chinese Chemical Letters2024，35，1： | 0 |
| 16 | OGT介导的糖修饰在Hippo通路激活和肿瘤发生中发挥重要作用显示文摘文章简介研究人员发现,肿瘤细胞可以响应细胞外葡萄糖浓度的变化,Hippo通路的核心蛋白YAP可以发生O-糖基化修饰。YAP的O-糖修饰由O糖基化转移酶OGT和糖苷酶OGA调控。 | Changmin Peng Yue Zhu Wanjun Zhang Qinchao Liao Yali Chen Xinyuan Zhao Qiang Guo Pan Shen Bei Zhen Xiaohong Qian Dong Yang Jin-San Zhang Dongguang Xiao 秦伟捷 裴华东 | 2018 | 科学新闻2018，0，4： | 0 |
| 17 | Modeling iron release from cast iron pipes in an urban water distribution system caused by source water switch显示文摘Significant iron release from cast iron pipes in water distribution systems(WDSs),which usually occurs during the source water switch period,is a great concern of water utilities because of the potential occurrence of'red water'and customer complaints.This study developed a new method which combined in-situ water stagnation experiments with mathematical models and numerical simulations to predict the iron release caused by source water switch.In-situ water stagnation experiments were conducted to determine the total iron accumulation in nine cast iron pipes in-service in Beijing when switching the local water to tre ated Danjiangkou Reservior water.Results showe d that the difference in the concentration increment of total iron in 24 hr(ΔCITI,24),i.e.short-term iron release,caused by source water switch was mainly dependent on the difference in the key quality parameters(pH,hardness,nitrate,Larson Ratio and dissolved oxygen(DO))between the two source waters.The iron release rate(RFe)after switch,i.e.long-term iron release,was closely related to the pipe properties as well as the DO and total residual chlorine(TRC)concentrations.Mathematical models ofΔCITI,24 and RFe were developed to quantitatively reveal the relationship between iron release and the key quality parameters.The RFe model could successfully combine with EPANET-MSX,a numerical simulator of water quality for WDSs to extend the iron release modeling from pipe level to network level.The new method is applicable to predicting iron release during source water switch,thus facilitating water utilities to take preventive actions to avoid'red water'. | Xiaodan Lin Qiang Xu Yuxian Li Bei Zhao Li Li Zhimin Qiang | 2021 | Journal of Environmental Sciences2021，33，12： | 0 |
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