|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Alteration of p53 and p21 during hepatocarcinogenesis in tree shrews显示文摘AIM: To investigate p53 mutation and p21 expression in hepatocarcinogenesis induced by hepatitis B virus (HBV) and aflatoxin B1 (AFB1) in tree shrews, and to reveal the role of these genes in hepatocarcinogenesis.METHODS: Tree shrews were divided into four groups:group A, those infected with HBV and fed with AFB1 (n = 39);group B, those infected with HBV alone (n = 28); group C,those fed with AFB1 alone (n = 29); and group D, normal controls (n = 20). The tree shrews underwent liver biopsies once every 15 wk. Expression of p53 and p21 proteins and genes in the biopsies and tumor tissues of the experimental tree shrews was detected, respectively, by immunohistochemistry,and by Southem blotting and reverse transcription-polymerase chain reaction and sequencing.RESULTS: The incidence of hepatocellular carcinomas (HCC) was higher in group A (66.7%) than that in group B (3.57%) and C (30%). The time of HCC occurrence was also earlier in group A than that in group C (120.0±16.6 wk vs 153.3±5.8 wk, respectively, P<0.01). p53 protein was not detected by immunohistochemistry in all groups before the 75^th wk of the experiment. At the 105^th wk, the positive rates fo p53 were 78.6%, 60% and 71.4% in groups A, B and C, respectively, which were significantly higher than that in group D (10%) (all P<0.05). An abnormal band of p53 gene was observed in groups A and C. The mutation points of p53gene in tree shrews with HCC were at codons 275, 78 and 13. The nucleotide sequence and amino acid sequence of tree shrew's wild-type p53 showed 91.7% and 93.4% homologies with those of human p53,respectively. The immunopositivity for p21 was found before HCC development. The incidence of HCC was significantly higher in tree shrews that were positive for p21 than those negative for p21 (80.0% vs 11.0%, P<0.001).The incidence of HCC in p21 positive animals in group A was significantly higher than those positive for p21 in group C (P | Jian-Jia Su Yuan Li Ke-Chen Ban Liu-Liang Qin Chun Yang Chao Ou Xiao-Xian Duan Hui-Yun Wang Rui-Qi Yang Young-Lk Lee | 2004 | World Journal of Gastroenterology2004，10，24： | 21 |
| 2 | Impact of Heat Wave in 2005 on Mortality in Guangzhou, China显示文摘Objective To assess the impact of the heat wave in 2005 on mortality among the residents in Guangzhou and to identify susceptible subpopulations in Guangzhou, China. Methods The data of daily number of deaths and meteorological measures from 2003 to 2006 in Guangzhou were used in this study. Heat wave was defined as ≥7 consecutive days with daily maximum temperature above 35.0 ℃ and daily mean temperature above the 97th percentile during the study period. The excess deaths and rate ratio (RR) of mortality in the case period compared with the reference period in the same summer were calculated. Results During the study period, only one heat wave in 2005 was identified and the total number of excess deaths was 145 with an average of 12 deaths per day. The effect of the heat wave on non-accidental mortality (RR=1.23, 95% CI: 1.11-1.37) was found with statistically significant difference. Also, greater effects were observed for cardiovascular mortality (RR=1.34, 95% CI: 1.13-1.59) and respiratory mortality (RR=1.31, 95% CI: 1.02-1.69). Females, the elderly and people with lower socioeconomic status were at significantly higher risk of heat wave-associatedmortality. Conclusion The 2005 heat wave had a substantial impact on mortality among the residents in Guangzhou, particularly among some susceptible subpopulations. The findings from the present study may provide scientific evidences to develop relevant public health policies and prevention measures aimed at reduction of preventable mortality from heat waves. | YANG Jun LIU Hua Zhang OU Chun Quan LIN Guo Zhen DING Yan ZHOU Qin SHEN Ji Chuan CHEN Ping Yan | 2013 | Biomedical and Environmental Sciences2013，26，8： | 16 |
| 3 | The Impacts of Mosquito Density and Meteorological Factors on Dengue Fever Epidemics in Guangzhou, China, 2006-2014: a Time-series Analysis显示文摘Objective To explore the associations between the monthly number of dengue fever(DF) cases and possible risk factors in Guangzhou, a subtropical city of China. Methods The monthly number of DF cases, Breteau Index(BI), and meteorological measures during 2006-2014 recorded in Guangzhou, China, were assessed. A negative binomial regression model was used to evaluate the relationships between BI, meteorological factors, and the monthly number of DF cases. Results A total of 39,697 DF cases were detected in Guangzhou during the study period. DF incidence presented an obvious seasonal pattern, with most cases occurring from June to November. The current month's BI, average temperature(Tave), previous month's minimum temperature(Tmin), and Tave were positively associated with DF incidence. A threshold of 18.25 °C was found in the relationship between the current month's Tmin and DF incidence. Conclusion Mosquito density, Tave, and Tmin play a critical role in DF transmission in Guangzhou. These findings could be useful in the development of a DF early warning system and assist in effective control and prevention strategies in the DF epidemic. | SHEN Ji Chuan LUO Lei LI Li JING Qin Long OU Chun Quan YANG Zhi Cong CHEN Xiao Guang | 2015 | Biomedical and Environmental Sciences2015，28，5： | 12 |
| 4 | LiFePO_4 doped with magnesium prepared by hydrothermal reaction in glucose solution显示文摘与镁做的锂铁磷酸盐(LiFePO4 ) 热水地在水溶液作为碳先锋与葡萄糖从商业 LiOH, FeSO4, H3PO4 和 MgSO4 被综合。样品被 X 光检查粉末衍射描绘,扫描电子显微检查法和骑车的经常的费用分泌物。结果证明综合粉末在与从葡萄糖的焦糖反应生产的碳先锋涂的 situ。在环境温度(28 ± 2 ° C ) ,准备的 LiFePO4 的电气化学的表演展出 135 mAh g 的高排出量 ? 1 在 5C 和超过 90 的 98% 的好能力保留骑车。优秀电气化学的表演应该与在碳和主要的 LiFePO4 之间的亲密接触被相关,源于,导致 LiFePO4 的电导率的增加并且二级粒子在里面 situ 积炭的形成先锋 / 碳。 | Xiu Qin Ou Guang Chuan Liang Jin Sheng Liang Sheng Zhao Xu Xia Zhao | 2008 | Chinese Chemical Letters2008，19，3： | 10 |
| 5 | Association of the polymorphisms of MMP-9 and TIMP-3 genes with thoracic aortic dissection in Chinese Han population显示文摘 | Xiao-long WANG Ou LIU Yan-wen QIN Hong-jia ZHANG Yi LV | 2014 | Acta Pharmacologica Sinica2014，35，3： | 6 |
| 6 | Dampness in dwellings and its associations with asthma and allergies among children in Chongqing: A cross-sectional study显示文摘The city of Chongqing has high outdoor temperatures and humidity throughout the year and consequently a high risk of dampness and mold problems in dwellings.As part of the China,Children,Homes,Health(CCHH),associations between home characteristics and children’s asthma,allergies,and related symptoms were investigated in Chongqing,China.A cross-sectional questionnaire survey on home characteristics including dampness and symptoms of asthma and allergies in 5299 children was performed.Data for 4754 children(3–6 years)and their homes were analyzed.Results showed that 35.1%of homes were reported to have'damp bedding',14.3%'condensation on window panes(higher than 5 cm)',11.6%'mouldy odor',9.3%'water leakage',8.3%'damp stains'and 5.3%'visible mould'.Wheezing and rhinitis ever were reported for 27.0%and 51.1%children respectively,and rhinitis,wheezing,cough at night and eczema in the last 12 months were reported for 38.1%,20.5%,18.9%and 13.2%respectively.Doctor-diagnosed asthma was reported for 8.3%of children,and doctor-diagnosed rhinitis for 6.2%.Dampness in homes was generally strongly associated with asthma and allergies among Chongqing children.The strongest association was found between'visible mould'and doctor-diagnosed rhinitis,and the adjusted Odds Ratio(ORA)was 2.27(95%CI:1.48,3.49).'Damp bedding'was significantly associated with all asthma and allergic symptoms,but doctor-diagnosed asthma and rhinitis.The behavior of'putting bedding to sunshine'could effectively reduce the prevalence of asthma and allergic symptoms,and the risk of bedding affected with damp was reduced significantly when bedding was put to sunshine frequently. | WANG Han LI BaiZhan YANG Qin YU Wei WANG Juan LIU YiLong OU YangJin SUNDELL Jan | 2013 | Chinese Science Bulletin2013，58，34： | 4 |
| 7 | A Note on Lower Bound of Centered L_2 -discrepancy on Combined Designs显示文摘This note provides a theoretical justification of optimal foldover plans in terms of uniformity. A new lower bound of the centered L2 -discrepancy values of combined designs is obtained, which can be used as a benchmark for searching optimal foldover plans. Our numerical results show that this lower bound is sharper than existing results when more factors reverse the signs in the initial design. | Yi Ju LEI Zu Jun OU Hong QIN Na ZOU | 2012 | Acta Mathematica Sinica,English Series2012，28，4： | 4 |
| 8 | A four-component combination derived from Huang-Qin Decoction significantly enhances anticancer activity of irinotecan显示文摘Huang-Qin Decoction(HQD)is a classic prescription for diarrhea in Chinese medicine treatment.Recent studies have demonstrated that HQD and its modified formulation PHY906 could ameliorate irinotecan(CPT-11)induced gastrointestinal(GI)toxicity and enhance its anticancer therapeutic efficacy.Nevertheless,which constituents in HQD are effective is still unclear so far.The study aims to screen out the key bioactive components combination from HQD that could enhance the anticancer effect of CPT-11.First,the potential bioactive constituents were obtained through system pharmacology strategy.Then the bioactivity of each constituent was investigated synthetically from the aspects of NCM460 cell migration,TNF-αrelease of THP-1-derived macrophage and MTT assay in HCT116 cell.The contribution of each constituent in HQD was evaluated using the bioactive index Ei,which taken the content and bioactivity into comprehensive consideration.And then,the most contributing constituents were selected out to form a keycomponent combination.At last,the bioefficacy of the key-component combination was validated in vitro and in vivo.As a result,a key-component combination(HB4)consisting of four compounds baicalin,baicalein,glycyrrhizic acid and wogonin was screened out.In vitro assessment indicated that HB4 could enhance the effect of CPT-11 on inhibiting cell proliferation and inducing apoptosis in HCT116.Furthermore,the in vivo study confirmed that HB4 and HQD have similar pharmacological activity and could both enhance the antitumor effect of CPT-11 in HCT116 xenograft model.Meanwhile,HB4 could also reduce the CPT-11 induced GI toxicity. | XU Dou-Dou HOU Xiao-Ying WANG Ou WANG Di LI Dan-Ting QIN Si-Yuan LV Bo DAI Xiao-Min ZHANG Zun-Jian WAN Jian-Bo XU Feng-Guo | 2021 | Chinese Journal of Natural Medicines2021，19，5： | 3 |
| 9 | Effects of Scutellaria Baicalensis on Activity and Biofilm Formation of Klebsiella Pneumoniae显示文摘Objective To explore the effects of Scutellaria baicalensis on activity and biofilm formation of Klebsiella pneumonia(Kp).Methods The broth and agar dilution methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of Scutellaria baicalensis for TW518.VITEK-32 system was used to assay TW518 susceptibility to antibiotics.Kp biofilms were formed in vitro and stained with Bac Light Live/Dead stain.The class integron geneⅠ1 m RNA expression was analyzed with RT-PCR.Results The minimum inhibitory concentration of Scutellaria baicalensis on TW518 identified as a Kp colony was 32 mg/ml,and minimum bactericidal concentration was 64 mg/ml.Scutellaria baicalensis and broad-spectrum penicillin,cephalosporin,quinolones,or beta-lactamase had synergistic bactericidal effects.Biofilm formation activity of Kp treated with Scutellaria baicalensis was significantly lower than that of the control group.And class integron geneⅠ1 m RNA expression of TW518 was significantly inhibited by Scutellaria baicalensis.Conclusions Scutellaria baicalensis has sterilization effect on Kp,and Scutellaria baicalensis could effectively inhibit Kp biofilm formation with prolonged treatment.Scutellaria baicalensis might inhibit Kp biofilm formation through down-regulating integron geneⅠ1 expression. | Wei Chen Bei Li Shuai Li Yi-wen Ou Qin Ou | 2016 | Chinese Medical Sciences Journal2016，31，3： | 3 |
| 10 | Modular construction mechanics of a European pressurized reactor steel containment liner显示文摘 | Jia-chuan YAN Xiao-fei JIN Feng QIN Zheng LI Feng FAN Jin-ping OU | 2017 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2017，18，6： | 2 |
| 11 | Infectivity of Severe Acute Respiratory Syndrome during Its Incubation Period显示文摘Objective To evaluate the infectivity of severe acute respiratory syndrome(SARS) during its incubation period by investigating chains of transmission and individuals isolated for medical observation with a view to providing scientific evidence for updating protocols of medical isolation.Methods Individuals related with the two SARS chains of transmission in Beijing in 2003 and a group of individuals isolated for medical observation in Haidian district of Beijing during the SARS outbreak were selected as subjects of study.Contactors with SARS patients and those with symptom development following the contacts were investigated via questionnaire.Serum samples were collected from super transmitters and tested for SARS-CoV antibody by neutralization test and enzyme linked immunosorbent assay(ELISA) .Results A total of 1 112 contactors were investigated in three surveys.Of them,669 had a history of close contact with symptomatic SARS patients,101 developed symptoms with a rate of 15.1%,363 had a history of close contact with patients in their incubation period,none of whom developed symptoms(0%) .Serum samples were collected from 32 highly-exposed individuals,of whom 13 developing SARS symptoms after contact had serum samples positive for SARS-CoV antibody.Samples collected from the asymptomatic contactors were all negative for SARS-CoV antibody.Conclusion SARS cases are infectious only during their symptomatic period and are non-infectious during the incubation period.Isolation for medical observation should be placed for individuals who are in close contact with symptomatic SARS patients.The results of our study are of decisive significance for the Ministry of Health to the definition of SARS close contactor. | GUANG ZENG SHU-YUN XIE QIN LI JIAN-MING OU | 2009 | Biomedical and Environmental Sciences2009，22，6： | 2 |
| 12 | Optimal foldover plans of three-level designs with minimum wrap-around L-_2 discrepancy显示文摘Abstract The objective of this paper is to study the issue of employing the uniformity criterion measured by the wrap-around L2-discrepancy to assess the optimal foldover plans for three-level designs.For three-level fractional factorials as the original designs,the general foldover plan and combined design under a foldover plan are defined,some theoretical properties of the defined foldover plans are obtained,a tight lower bound of the wrap-around L2-discrepancy of combined designs under a general foldover plan is also obtained,which can be used as a benchmark for searching optimal foldover plans.For illustration of the usage of our theoretical results,a catalog of optimal foldover plans for uniform initial designs with s three-level factors is tabulated,where 2≤ s ≤11. | OU ZuJun QIN Hong CAI Xu | 2015 | Science China Mathematics2015，58，7： | 2 |
| 13 | Effect of garlic on micronucleus frequency of peripheral blood lymphocytes in MNNG-induced gastric carcinoma and precancerous lesion in rats显示文摘EfectofgarliconmicronucleusfrequencyofperipheralbloodlymphocytesinMNNGinducedgastriccarcinomaandprecancerouslesioninratsSUQi?.. | SU Qi, LUO ZhaoYang, OU YangGui, LI YiQin, ZHOU JianGuo and ZHANG DanInstitute of Oncology, Hengyang Medical College, Hengyang 421001, Hunan Province, China | 1997 | World Journal of Gastroenterology1997，3，4： | 2 |
| 14 | Expert consensus on the perioperative management of patients with sepsis显示文摘INTRODUCTION Sepsis is defined as an infection associated with systemic manifestations of inflammation,which has become the most common complication in the perioperative period caused by severe burn/trauma and major surgical operation.^([1,2])The incidence of sepsis in adults is estimated to be 149–240/100 000 per year,and that of severe sepsis and septic shock is 56–91/100 000per year.In the last decade,the short-term | Jun-ping Chen Xiang-ming Fang Xiao-ju Jin Rong-tian Kang Ke-xuan Liu Jin-bao Li Yan Luo Zhi-jie Lu Chang-hong Miao Han-xiang Ma Wei Mei Yang-wen Ou Si-hua Qi Zai-sheng Qin Guo-gang Tian An-shi Wu Dong-xin Wang Tian Yu Yong-hao Yu Jing Zhao Ming-zhang Zuo Shi-hai Zhang | 2015 | World Journal of Emergency Medicine2015，6，4： | 2 |
| 15 | Role of autolysin mediated DNA re- lease in biofilm formation of Staphylococcus epidermidis显示文摘 | Qin Z Ou Y Yang L | 2007 | Mi- crobiology2007，153，7： | 1 |
| 16 | Some applications of indicator function in two-level factorial designs显示文摘 | Ou Z J Qin H | 2010 | Statist Probab Lett2010，80，： | 1 |
| 17 | Role of autolysin-mediated DNA release in biofilm formation of Staphylococcus epidermidis显示文摘 | Qin ZQ Ou YZ Yang L | 2007 | Microbiology2007，153，： | 1 |
| 18 | In-situ stress measurements and regional stress field assessment of the Beishan area, China显示文摘 | X.G. Zhao J. Wang M. Cai L.K. Ma Z.H. Zong X.Y. Wang R. Su W.M. Chen H.G. Zhao Q.C. Chen Q.M. An X.H. Qin M.Y. Ou J.S. Zhao | 2013 | Engineering Geology2013，，： | 1 |
| 19 | Improved efficacy of chemotherapy for glioblastoma by radiation-induced opening of blood-brain barrier:clinical results显示文摘 | QIN D OU G MO H | 2001 | Int J Radiat Oncol Biol Phys2001，51，4： | 1 |
| 20 | Antimicrobial activities of a new formula of spice water extracts against foodborne bacteria显示文摘 | Qin Wang Zibian Ou Hanwu Lei | 2012 | Journal of food processing and preservation2012，36，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员