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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Genomic structure of metabotropic glutamate receptor 7 and comparison of genomic structures of extracellular domains of mGluR family显示文摘Metabotropic glutamate receptor 7, coupled with a chemical neurotransmitter L-glutamate, plays an important role in the development of many psychiatric and neurological disorders. To study the biological and genetic mechanism of the mGluR7-related diseases, a physical map covering the full-length mGluR7 genomic sequence has been constructed through seed clone screening and fingerprinting database searching. These BAC clones in the physical map have been sequenced with shotgun strategy and assembled by Phred-Phrap-Consed software; the error rate of the final genoniic sequence is less than 0.01%. mGluR7 spans 880 kb genoniic region, the GC content and repeat content of mGluR7 genoniic sequence are 38% and 37.5% respectively. mGluR7 has a typical 'house-keeping' promoter and consists of 11 exons, with introns ranging from 6 kb to 285 kb. mGluR7a and mGluR7b are two known alternatively splicing variants. Comparing the genomic structures of extracellular domains of mGluR family, their genomic structures can | Qingfa Wu Wei Dong Xiaohua Qi Weidong Bao Yuxin Niu Yiling Zhang Haiqing Zhang Chong Chen Bin Liu Songnian Hu et al. | 2002 | Chinese Science Bulletin2002，47，16： | 4 |
| 3 | CCAR1 5' UTR as a natural miRancer of miR-1254 overrides tamoxifen resistance显示文摘 | Gaopeng Li Xiaoli Wu Wenchang Qian Huayong Cai Xinbao Sun Weijie Zhang Sheng Tan Zhengsheng Wu Pengxu Qian Keshuo Ding Xuefei Lu Xiao Zhang Hong Yan Haifeng Song Shouhong Guang Qingfa Wu Peter E Lobie Ge Shan Tao Zhu | 2016 | Cell Research2016，26，6： | 3 |
| 4 | Role of humidity on the structures and properties of regenerated silk fibers显示文摘Silk fiber was processed from highly concentrated spinning dope to solid fibers along with water removal. To understand the mechanism of water removal during silk fiber spinning process, a microfluidic chip was designed and applied to investigate the structures and mechanical properties of two kinds of regenerated silk fibroin fibers dry-spun at different relative humidity. The experimental results showed that the diameters of the fibers spun at 40% RH are always larger than the fibers spun at 50% RH due to different removal rates of water. The fibers spun at low humidity contain more β-sheet structure and lower degree of chain orientation and crystalline orientation. These results indicate that the fast phase transition of silk fibroin from sol–gel to silk fiber undergoes with rapid water removal and higher fiber orientation relates to more residue water and drawing force. | Qingfa Peng Huili Shao Xuechao Hu Yaopeng Zhang | 2015 | Progress in Natural Science:Materials International2015，25，5： | 3 |
| 5 | AlCl 3 -promoted MCM-41-supported platinum catalysts with high activity and sulfur-tolerance for tetralin hydrogenation: Effect of Pt–Al interaction显示文摘 | Mingjian Luo Qingfa Wang Guozhu Li Xiangwen Zhang Li Wang Lijun Han | 2013 | Catalysis Communications2013，，： | 1 |
| 6 | Study on deactivation and regeneration of Pd/Al2O3 catalyst in hydrogen peroxide production by the anthraquinone process显示文摘 | Qingfa Wang Li Wang Yaquan Wang Fei He Zhenhua Li Zhentao Mi | 2004 | Reaction Kinetics and Catalysis Letters2004，，2： | 1 |
| 7 | Preparative isolation and purification of phenolic acids from Smilax china by high-speed countercurrent chromatography显示文摘 | Chunhua Yanga Qingfa Tang Jinghan l iu | 2008 | Separation and Purification Technology2008，61，： | 1 |
| 8 | Psychosocial predictors of smoking among secondary school students in Henan,China显示文摘 | ZHANG Lian WANG Weifeng ZHAO Qingfa | 2000 | Health Edo Res2000，15，： | 1 |
| 9 | Psychosocial predictors of smoking among secondary school students in Henan,China显示文摘 | Lian Zhang Weifeng Wang Qingfa Zhao | 2000 | Health Education Research2000，15，4： | 1 |
| 10 | Thermal Stability and Kinetic of Decomposition of Nitra- ted HTPB 显示文摘 | WANG Qingfa WANG Li ZHANG Xiangwen | 2009 | Journal of Hazardous Materials2009，172，23： | 1 |
| 11 | Development of stem cell-based therapy for Parkinson’s disease显示文摘Parkinson’s disease(PD)is one of the most common neurodegenerative disorders of aging,characterized by the degeneration of dopamine neurons(DA neurons)in the substantial nigra,leading to the advent of both motor symptoms and non-motor symptoms.Current treatments include electrical stimulation of the affected brain areas and dopamine replacement therapy.Even though both categories are effective in treating PD patients,the disease progression cannot be stopped.The research advance into cell therapies provides exciting potential for the treatment of PD.Current cell sources include neural stem cells(NSCs)from fetal brain tissues,human embryonic stem cells(hESCs),induced pluripotent stem cells(iPSCs)and directly induced dopamine neurons(iDA neurons).Here,we evaluate the research progress in different cell sources with a focus on using iPSCs as a valuable source and propose key challenges for developing cells suitable for large-scale clinical applications in the treatment of PD. | Fabin Han Deborah Baremberg Junyu Gao Jing Duan Xianjie Lu Nan Zhang Qingfa Chen | 2015 | Translational Neurodegeneration2015，4，1： | 1 |
| 12 | Synthesis of a novel biodegradable poly (ester amine) (PEAs) copolymer based on low-molecular-weight polyethyleneimine for gene delivery显示文摘 | Guo QingFa Shi Shuai Wang XiuHong | | 0，，01： | 1 |
| 13 | Research Progress in the Regulation of Tumor Cells and Tumor Stem Cells at Multiple Targets by Antrodia camphorata显示文摘Extensive in vitro and in vivo research reveals multiple intracellular molecular targets of Antrodia camphorata,and these targets affect growth,apoptosis,angiogenesis,invasion and metastasis of cells.These targets include tumor suppressor,cell cycle regulator,transcription factor,angiogenesis and metastasis factor,apoptosis and survival regulator,etc.Additionally,more and more attention has been paid to the molecular mechanism of A.camphorata on the regulation of tumor stem cells.Meanwhile,there is evidence that the immunoregulation of A.camphorata is enhanced,which may lead cell cycle arrest or apoptosis.In this paper,molecular mechanism of tumor cells and tumor stem cells regulated at multiple targets by A.camphorata in vitro and in vivo in the past decade is summarized. | Qingfa CHEN Yan XU Xiaodong SHI Chuanfei WEI HaitaoXIE Ruxi LV | 2020 | Medicinal Plant2020，11，3： | 1 |
| 14 | Facilitated transport of SO2 through supported liquid membrane using water as a carrier 显示文摘 | Teramoto Masaaki Huang Qingfa Maki Taisuke | 1999 | Separation and Purfication Technology1999，16，： | 1 |
| 15 | Study on deactivation and regeneration of Pd/Al2O3 catalyst in Hydrogen peroxide production by the anthraquinone process显示文摘 | Wang Qingfa Wang Li Wang Yaquan | 2004 | React Kinet Catal Lett2004，81，2： | 1 |
| 16 | Tough silk fibers prepared in air using a biomimetic microfluidic chip显示文摘 | Jie Luo Lele Zhang Qingfa Peng Mengjie Sun Yaopeng Zhang Huili Shao Xuechao Hu | 2014 | International Journal of Biological Macromolecules2014，，： | 1 |
| 17 | Numerical simulation of dimension effect to deformation in the cataclastic medium goafs using UDEC显示文摘 | HU Jianhua LAO Dezheng CHEN Qingfa | 2010 | Lecture Notes in Computer Science2010，5938，： | 1 |
| 18 | Metal-Free Synthesis of β-Bromoalkenyl Sulfides via Deoxygenative Bromination/Olefination/Sulfenylation of Ketones with SulfonylHydrazides and Pyridinium Tribromide显示文摘A novel metal-free method for synthesis of β-bromoalkenyl sulfides via deoxygenative bromination/olefination/sulfenylation process usingcommercially available ketones, sulfonyl hydrazides and pyridinium tribromide as starting materials has been developed. In this reaction, pyridiniumtribromide plays the role of oxidant and substrate, wherein water and molecular nitrogen are generated as environmentally benign by-products. Prelimi-nary investigation revealed that vinyl bromides were critical intermediate. Importantly, this protocol not only obviates the use of alkynes and traditionalsulfenylating agents, but also reveals that ketones can be used as precursors of vinyl bromides. | Yishu Bao Lingyu Zhong Qiaodan Hou Qingfa Zhou Fulai Yang | 2018 | Chinese Journal of Chemistry2018，36，11： | 0 |
| 19 | A host-guest approach to fabricate metallic cobalt nanoparticles embedded in silk-derived N-doped carbon fibers for efficient hydrogen evolution显示文摘Hydrogen evolution reaction(HER) plays a key role in generating clean and renewable energy. As the most effective HER electrocatalysts, Pt group catalysts suffer from severe problems such as high price and scarcity. It is highly desirable to design and synthesize sustainable HER electrocatalysts to replace the Pt group catalysts. Due to their low cost, high abundance and high activities, cobalt-incorporated N-doped nanocarbon hybrids are promising candidate electrocatalysts for HER. In this report, we demonstrated a robust and eco-friendly host-guest approach to fabricate metallic cobalt nanoparticles embedded in N-doped carbon fibers derived from natural silk fibers. Benefiting from the onedimensional nanostructure, the well-dispersed metallic cobalt nanoparticles and the N-doped thin graphitized carbon layer coating, the best Cobased electrocatalyst manifests low overpotential(61 mV@10 mA/cm^2) HER activity that is comparable with commercial 20% Pt/C, and good stability in acid. Our findings provide a novel and unique route to explore high-performance noble-metal-free HER electrocatalysts. | Fenglei Lyu Qingfa Wang Han Zhu Mingliang Du Li Wang Xiangwen Zhang | 2017 | Green Energy & Environment2017，2，2： | 0 |
| 20 | Molecular basis and mechanism of action of Albizia julibrissin in depression treatment and clinical application of its formulae显示文摘Albizzia julibrissin is empirically used as an antidepressant in clinical practice.Preclinical studies have indicated that its total extracts or bioactive constituents exerted antidepressant-like responses in animal models,providing the molecular basis to reveal its underlying mechanism of action.While attempts have been made to understand the antidepressant effect of A.julibrissin,many fundamental questions regarding its mechanism of action remain to be addressed at the molecular and systems levels.In this review,we conclusively discussed the mechanism of action of A.julibrissin and A.julibrissin formulae by reviewing recent preclinical and clinical studies conducted by using depressive animal models and depressive patients.Several representative bioactive constituents and formulae were highlighted as examples,and their mechanisms of action were discussed.In addition,some representative A.julibrissin formulae that have been shown to be compatible with conventional antidepressants in clinical practice were also reviewed.Furthermore,we discussed the future research directions to reveal the underlying mechanism of A.julibrissin at the molecular and systems levels in depression treatment.The integrated study using both the molecular and systematic approaches is required not only for improving our understanding of its molecular basis and mechanisms of action,but also for providing a way to discover novel agents or approaches for the effective and systematic treatment of depression. | Bishan Huang Yingyao Wu Chan Li Qingfa Tang Yuanwei Zhang | 2023 | Chinese Herbal Medicines2023，15，2： | 0 |
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