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| 1 | Recent progress in engineering near-infrared persistent luminescence nanoprobes for time-resolved biosensing/bioimaging显示文摘Persistent luminescence nanoprobes (PLNPs) can remain luminescent after ceasing excitation.Due to the ultra-long decay time of persistent luminescence (PersL),autofluorescence interference can be efficiently eliminated by collecting PersL signal after autofluorescence decays completely,thus the imaging contrast and sensing sensitivity can be significantly improved.Since near-infrared (NIR) light shows reduced scattering and absorption coefficient in penetrating biological organs or tissues,near-infrared persistent luminescence nanoprobes (NIR PLNPs) possess deep tissue penetration and offer a bright prospect in the areas of in vivo biosensing/bioimaging.In this review,we firstly summarize the design of different types of NIR PLNPs for biosensing/bioimaging,such as transition metal ions-doped NIR PLNPs,lanthanide ions-doped NIR PLNPs,organic molecules-based NIR PLNPs,and semiconducting polymer self-assembled NIR PLNPs.Notably,organic molecules-based NIR PLNPs and semiconductor self-assembled NIR PLNPs,for the first time,were introduced to the review of PLNPs.Secondly,the effects of different types of charge carriers on NIR PersL and luminescence decay of NIR PLNPs are significantly emphasized so as to build up an in-depth understanding of their luminescence mechanism.It includes the regulation of valence band and conduction band of different host materials,alteration of defect types,depth and concentration changes caused by ion doping,effective radiation transitions and energy transfer generated by different luminescence centers.Given the design and potential of NIR PLNPs as long-lived luminescent materials,the current challenges and future perspective in this rapidly growing field are also discussed. | Ling Liang Na Chen Yiyi Jia Qinqin Ma Jie Wang Quan Yuan Weihong Tan | 2019 | Nano Research2019，12，6： | 11 |
| 2 | Pseudomonas aeruginosa:pathogenesis,virulence factors,antibiotic resistance,interaction with host,technology advances and emerging therapeutics显示文摘Pseudomonas aeruginosa(P.aeruginosa)is a Gram-negative opportunistic pathogen that infects patients with cystic fibrosis,burn wounds,immunodeficiency,chronic obstructive pulmonary disorder(COPD),cancer,and severe infection requiring ventilation,such as COVID-19.P.aeruginosa is also a widely-used model bacterium for all biological areas.In addition to continued,intense efforts in understanding bacterial pathogenesis of P.aeruginosa including virulence factors(LPS,quorum sensing,two-component systems,6 type secretion systems,outer membrane vesicles(OMVs),CRISPR-Cas and their regulation),rapid progress has been made in further studying host-pathogen interaction,particularly host immune networks involving autophagy,inflammasome,noncoding RNAs,cGAS,etc.Furthermore,numerous technologic advances,such as bioinformatics,metabolomics,scRNA-seq,nanoparticles,drug screening,and phage therapy,have been used to improve our understanding of P.aeruginosa pathogenesis and host defense.Nevertheless,much remains to be uncovered about interactions between P.aeruginosa and host immune responses,including mechanisms of drug resistance by known or unannotated bacterial virulence factors as well as mammalian cell signaling pathways.The widespread use of antibiotics and the slow development of effective antimicrobials present daunting challenges and necessitate new theoretical and practical platforms to screen and develop mechanism-tested novel drugs to treat intractable infections,especially those caused by multi-drug resistance strains.Benefited from has advancing in research tools and technology,dissecting this pathogen’s feature has entered into molecular and mechanistic details as well as dynamic and holistic views.Herein,we comprehensively review the progress and discuss the current status of P.aeruginosa biophysical traits,behaviors,virulence factors,invasive regulators,and host defense patterns against its infection,which point out new directions for future investigation and add to the design of novel and/or alternative therapeutics to combat this clinically significant pathogen. | Shugang Qin Wen Xiao Chuanmin Zhou Qinqin Pu Xin Deng Lefu Lan Haihua Liang Xiangrong Song Min Wu | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 5 |
| 3 | An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte–tumor cell crosstalk,local immunosuppression and tumor progression显示文摘Dissecting and understanding the cancer ecosystem,especially that around the tumor margins,which have strong implications for tumor cell infiltration and invasion,are essential for exploring the mechanisms of tumor metastasis and developing effective new treatments.Using a novel tumor border scanning and digitization model enabled by nanoscale resolution-SpaTial Enhanced REsolution Omics-sequencing(Stereo-seq),we identified a 500µm-wide zone centered around the tumor border in patients with liver cancer,referred to as“the invasive zone”.We detected strong immunosuppression,metabolic reprogramming,and severely damaged hepatocytes in this zone.We also identified a subpopulation of damaged hepatocytes with increased expression of serum amyloid A1 and A2(referred to collectively as SAAs)located close to the border on the paratumor side.Overexpression of CXCL6 in adjacent malignant cells could induce activation of the JAK-STAT3 pathway in nearby hepatocytes,which subsequently caused SAAs’overexpression in these hepatocytes.Furthermore,overexpression and secretion of SAAs by hepatocytes in the invasive zone could lead to the recruitment of macrophages and M2 polarization,further promoting local immunosuppression,potentially resulting in tumor progression.Clinical association analysis in additional five independent cohorts of patients with primary and secondary liver cancer(n=423)showed that patients with overexpression of SAAs in the invasive zone had a worse prognosis.Further in vivo experiments using mouse liver tumor models in situ confirmed that the knockdown of genes encoding SAAs in hepatocytes decreased macrophage accumulation around the tumor border and delayed tumor growth.The identification and characterization of a novel invasive zone in human cancer patients not only add an important layer of understanding regarding the mechanisms of tumor invasion and metastasis,but may also pave the way for developing novel therapeutic strategies for advanced liver cancer and other solid tumors. | Liang Wu Jiayan Yan Yinqi Bai Feiyu Chen Xuanxuan Zou Jiangshan Xu Ao Huang Liangzhen Hou Yu Zhong Zehua Jing Qichao Yu Xiaorui Zhou Zhifeng Jiang Chunqing Wang Mengnan Cheng Yuan Ji Yingyong Hou Rongkui Luo Qinqin Li Liang Wu Jianwen Cheng Pengxiang Wang Dezhen Guo Waidong Huang Junjie Lei Shang Liu Yizhen Yan Yiling Chen Sha Liao Yuxiang Li Haixiang Sun Na Yao Xiangyu Zhang Shiyu Zhang Xi Chen Yang Yu Yao Li Fengming Liu Zheng Wang Shaolai Zhou Huanming Yang Shuang Yang Xun Xu Longqi Liu Qiang Gao Zhaoyou Tang Xiangdong Wang Jian Wang Jia Fan Shiping Liu Xinrong Yang Ao Chen Jian Zhou | 2023 | Cell Research2023，33，8： | 1 |
| 4 | On Skew Strongly Reversible Rings Relative to a Monoid显示文摘For a monoid M, we introduce the concept of skew strongly M-reversible rings which is a generalization of strongly M-reversible rings, and investigate their properties. It is shown that if G is a finitely generated Abelian group, then G is torsion-free if and only if there exists a ring R with |R| ≥ 2 such that R is skew strongly G-reversible. Moreover, we prove that if R is a right Ore ring with classical right quotient ring Q, then R is skew strongly M-reversible if and only if Q is skew strongly M-reversible. | Zhaiming PENG Qinqin GU Liang ZHAO | 2016 | Journal of Mathematical Research with Applications2016，36，1： | 1 |
| 5 | Probingthe interaction of magnetic iron oxide nanoParticleswith bovine serum albumin by sPectroscoPic techniques显示文摘 | Yang Qinqin Liang Jiangong Han Heyou | 2009 | J Phys Chem B2009，113，10： | 1 |
| 6 | Gene2DGE: A Perl Package for Gene Model Renewal with Digital Gene Expression Data显示文摘For transcriptome analysis, it is critical to precisely define all the transcripts across the whole genome. More and more digital gene expression (DGE) scannings have indicated the presence of huge amount of novel transcripts in addition to the known gene models. However, almost all these studies still depend crucially on existing annotation. Here, we present Gene2DGE, a Perl software package for gene model renewal with DGE data. We applied Gene2DGE to the mouse blastomere transcriptome, and defined 98,532 read-enriched regions (RERs) by read clustering supported by more than four reads for each base pair. Taking advantage of this ab initio method, we refined 2,104 exonic regions (4% of a total of 48,501 annotated transcribed regions) with remarkable extension into un-annotated regions (>50 bp). For 5% of uniquely mapped reads falling within intron regions, we identified 13,291 additional possible exons. As a result, we renewed 4,788 gene models, which account for 39% of a total of 12,277 transcribed genes. Furthermore, we identified 12,613 intergenic RERs, suggesting the possible presence of novel genes outside the existing gene models. In this study, therefore, we have developed a suitable tool for re-newal of known gene models by ab initio prediction in transcriptome dissection. The Gene2DGE package is freely available at http://gffzz7910605137224006hxufwvbnp0b6n6pn9.ffgz.tsg.suse.edu.cn/. | Xiaoli Tang Libin Deng Dake Zhang Jiari Lin Yi Wei Qinqin Zhou Xiang Li Guilin Li Shangdong Liang | 2012 | Genomics, Proteomics & Bioinformatics2012，10，1： | 0 |
| 7 | Formation of secondary inorganic aerosol in a frigid urban atmosphere显示文摘Formation of secondary inorganic aerosol(SIA)was investigated during a six-month long heating season in Harbin,China.Enhanced sulfate formation was observed at high relative humidity(RH),with the same threshold RH(80%)for both colder and warmer measurement periods.Compared to wintertime results from Beijing,the threshold RH was considerably higher in Harbin,whereas the RH-dependent enhancement of sulfur oxidation ratio(SOR)was less significant.In addition,the high RH events were rarely encountered,and for other periods,the SOR were typically as low as~0.1.Therefore,the sulfate formation was considered inefficient in this study.After excluding the several cases with high RH,both SOR and the nitrogen oxidation ratio(NOR)exhibited increasing trends as the temperature increased,with the increase of NOR being sharper.The nitrate to sulfate ratio tended to increase with increasing temperature as well.Based on a semi-quantitative approach,this trend was attributed primarily to the temperature-dependent variations of precursors including SO_(2) and NO_(2).The influence of biomass burning emissions on SIA formation was also evident.With stronger impact of biomass burning,an enhancement in NOR was observed whereas SOR was largely unchanged.The different patterns were identified as the dominant driver of the larger nitrate to sulfate ratios measured at higher concentrations of fine particulate matter. | Yuan Cheng Qinqin Yu Jiumeng Liu Youwen Sun Linlin Liang Zhenyu Du Guannan Geng Wanli Ma Hong Qi Qiang Zhang Kebin He | 2022 | Frontiers of Environmental Science & Engineering2022，16，2： | 0 |
| 8 | Hypercompact CRISPR–Cas12j2 (CasF) enables genome editing, gene activation, and epigenome editing in plants显示文摘CRISPR-Cas9,-Cas12a,-Cas12b,and-Cas13 have been harnessed for genome engineering in human and plant cells(Liu et al.,2022).However,the large size of these Cas proteins(e.g.190 kDa for SpCas9)makes them difficult to deliver into cells via a viral vector.The development of smaller Cas proteins will lead to reduced viral vector sizes that can be more widely adopted in versatile genome engineering systems.Recently,a CRISPR-Cas12j2(CasF)system was discovered in huge phages and developed into a hypercompact genome editor due to the small size of Cas12j2(80 kDa)(Pausch et al.,2020).Unfortunately,the gene editing efficiency of Cas12j2 in Arabidopsis protoplasts using ribonucleoprotein delivery was less than one percent(Pausch et al.,2020).Further optimization of this system is clearly required if CRISPR-Cas12j2-mediated editing in plant genomes is to be adopted by the plant sciences community. | Shishi Liu Simon Sretenovic Tingting Fan Yanhao Cheng Gen Li Aileen Qi Xu Tang Yang Xu Weijun Guo Zhaohui Zhong Yao He Yanling Liang Qinqin Han Xuelian Zheng Xiaofeng Gu Yiping Qi Yong Zhang | 2022 | Plant Communications2022，3，6： | 0 |
| 9 | Genomic basis of selective breeding from the closest wild relative of large-fruited tomato显示文摘The long and intricate domestication history of the tomato(Solanum lycopersicum)includes selection sweeps that have not been fully explored,and these sweeps show significant evolutionary trajectories of domestication traits.Using three distinct selection strategies,we represented comprehensive selected sweeps from 53 Solanum pimpinellifolium(PIM)and 166 S.lycopersicum(BIG)accessions,which are defined as pseudo-domestication in this study.We identified 390 potential selection sweeps,some of which had a significant impact on fruit-related traits and were crucial to the pseudo-domestication process.During tomato pseudo-domestication,we discovered a minor–effect allele of the SlLEA gene related to fruit weight(FW),as well as the major haplotypes of fw2.2/cell number regulator(CNR),fw3.2/SlKLUH,and fw11.3/cell size regulator(CSR)in cultivars.Furthermore,18 loci were found to be significantly associated with FW and six fruit-related agronomic traits in genome-wide association studies.By examining population differentiation,we identified the causative variation underlying the divergence of fruit flavonoids across the large-fruited tomatoes and validated BRI1-EMS-SUPPRESSOR 1.2(SlBES1.2),a gene that may affect flavonoid content by modulating the MYB12 expression profile.Our results provide new research routes for the genetic basis of fruit traits and excellent genomic resources for tomato genomics-assisted breeding. | Junwei Yang Yun Liu Bin Liang Qinqin Yang Xuecheng Li Jiacai Chen Hongwei Li Yaqing Lyu Tao Lin | 2023 | Horticulture Research2023，10，8： | 0 |
| 10 | Layer-by-layer epitaxy of multi-layer MoS_(2) wafers显示文摘The 2D semiconductor of MoShas great potential for advanced electronics technologies beyond silicon.So far,high-quality monolayer MoSwafers have been available and various demonstrations from individual transistors to integrated circuits have also been shown.In addition to the monolayer,multilayers have narrower band gaps but improved carrier mobilities and current capacities over the monolayer.However,achieving high-quality multi-layer MoSwafers remains a challenge.Here we report the growth of high-quality multi-layer Mo S_(2) 4-inch wafers via the layer-by-layer epitaxy process.The epitaxy leads to well-defined stacking orders between adjacent epitaxial layers and offers a delicate control of layer numbers up to six.Systematic evaluations on the atomic structures and electronic properties were carried out for achieved wafers with different layer numbers.Significant improvements in device performances were found in thicker-layer field-effect transistors(FETs),as expected.For example,the average field-effect mobility(μFE)at room temperature(RT)can increase from~80 cm·V·sfor monolayers to~110/145 cm·V·for bilayer/trilayer devices.The highest RTμFEof 234.7 cm·V·sand record-high on-current densities of 1.70 m A·μmat Vds=2V were also achieved in trilayer MoSFETs with a high on/off ratio of>107.Our work hence moves a step closer to practical applications of 2D MoSin electronics. | Qinqin Wang Jian Tang Xiaomei Li Jinpeng Tian Jing Liang Na Li Depeng Ji Lede Xian Yutuo Guo Lu Li Qinghua Zhang Yanbang Chu Zheng Wei Yanchong Zhao Luojun Du Hua Yu Xuedong Bai Lin Gu Kaihui Liu Wei Yang Rong Yang Dongxia Shi Guangyu Zhang | 2022 | National Science Review2022，9，6： | 0 |
| 11 | Structure-guided engineering of transcriptional activator XYR1 for inducer-free production of lignocellulolytic enzymes in Trichoderma reesei显示文摘The filamentous fungus Trichoderma reesei is widely used for the production of lignocellulolytic enzymes in industry.XYR1 is the major transcriptional activator of cellulases and hemicellulases in T.reesei.However,rational engineering of XYR1 for improved lignocellulolytic enzymes production has been limited by the lack of structure information.Here,alanine 873 was identified as a new potential target for the engineering of XYR1 based on its structure predicted by AlphaFold2.The mutation of this residue to tyrosine enabled significantly enhanced production of xylanolytic enzymes in the medium with cellulose as the carbon source.Moreover,xylanase and cellulase production increased by 56.7-and 3.3-fold,respectively,when glucose was used as the sole carbon source.Under both conditions,the improvements of lignocellulolytic enzyme production were higher than those in the previously reported V821F mutant.With the enriched hemicellulases and cellulases,the crude enzymes secreted by the A873Y mutant strain produced 51%more glucose and 52%more xylose from pretreated corn stover than those of the parent strain.The results provide a novel strategy for engineering the lignocellulolytic enzyme-producing capacity of T.reesei,and would be helpful for understanding the molecular mechanisms of XYR1 regulation. | Qinqin Zhao Zezheng Yang Ziyang Xiao Zheng Zhang Jing Xing Huiqi Liang Liwei Gao Jian Zhao Yinbo Qu Guodong Liu | 2023 | Synthetic and Systems Biotechnology2023，8，4： | 0 |
| 12 | AlCl_(3)modified Pd/Al_(2)O_(3)catalyst for enhanced anthraquinone hydrogenation显示文摘Anthraquinone hydrogenation to produce H_(2)O_(2) is an economically interesting reaction with great industrial importance.Here,we report a series of Pd/xAl catalysts with different AlCl_(3) contents by a conventional stepwise impregnation method.The optimal Pd/1.0Al catalyst exhibits a higher performance toward anthraquinone hydrogenation with 8.3 g·L^(-1)hydrogenation efficiency,99.5%selectivity and good stability,obviously superior to that of Pd/Al_(2)O_(3) catalyst(5.2 g·L^(-1)and 97.2%).Detailed characterization demonstrates that AlCl_(3) can be grafted on the γ-Al_(2)O_(3) support to obtain a modified support with abundant surface weak acid and Lewis acid,which can adsorb and activate anthraquinone.Meanwhile,its steric hindrance could isolate and disperse active metals to form more active sites.The synergies between metal sites and acid sites promotes the anthraquinone hydrogenation.Furthermore,the good stability after grafting AlCl_(3) could attribute to the enhanced metal-support interaction inhibiting metal particles agglomeration and leaching. | Qinqin Yuan Jingyue Liang Wei Li Jinli Zhang Cuili Guo | 2023 | Chinese Journal of Chemical Engineering2023，64，12： | 0 |
| 13 | Cu-doped SnO_(2)/rGO nanocomposites for ultrasensitive H2S detection at low temperature显示文摘Hydrogen sulfide(H_(2)S)detection remains a significant concern and the sensitivity,selectivity,and detection limit must be balanced at low temperatures.Herein,we utilized a facile solvothermal method to prepare Cu-doped SnO_(2)/rGO nanocomposites that have emerged as promising candidate materials for H_(2)S sensors.Characterization of the Cu-SnO_(2)/rGO was carried out to determine its surface morphology,chemical composition,and crystal defects.The optimal sensor response for 10 ppm H_(2)S was~1415.7 at 120℃,which was over 320 times higher than that seen for pristine SnO_(2)CQDs(R_(a)/R_(g)=4.4)at 280℃.Moreover,the sensor material exhibited excellent selectivity,a superior linear working range(R^(2)=0.991,1-150 ppm),a fast response time(31 s to 2 ppm),and ppb-level H_(2)S detection(R_(a)/R_(g)=1.26 to 50 ppb)at 120℃.In addition,the sensor maintained a high performance even at extremely high humidity(90%)and showed outstanding long-term stability.These superb H_(2)S sensing properties were attributed to catalytic sensitization by the Cu dopant and a synergistic effect of the Cu-SnO_(2)and rGO,which offered abundant active sites for O_(2)and H_(2)S absorption and accelerated the transfer of electrons/holes. | Tingting Chen Jianhai Sun Ning Xue Wen Wang Zongchang Luo Qinqin Liang Tianye Zhou Hao Quan Haoyuan Cai Kangsong Tang Kaisheng Jiang | 2023 | Microsystems & Nanoengineering2023，9，3： | 0 |
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