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| 1 | Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9- Guided Hyperactive hAID Mutant显示文摘 | Bin Ren Fang Yan Yongjie Kuang Na Li Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 46 |
| 2 | Base-Editing-Mediated Artificial Evolution of OsALS1 In Planta to Develop Novel Herbicide-Tolerant Rice Germplasms显示文摘Recently developed CRISPR-mediated base editors,which enable the generation of num erous nucleotide changes in target genomic regions,have been widely adopted for gene correction and generation of crop germ plasms containing im portant gain-of-function genetic variations.How ever,to engineer target genes with unknown functional SNPs remains challenging.To address this issue,we present here abase-e diting-mediated gene evolution(BEMGE)m ethod,employing both Cas9n-based cytosine and adenine base editors as well as a single-guide RNA(sgRNA)library tiling the full-length coding region,for developing novel rice germ plasm swith mutations in any endogenous gene.To this end,OsALS1 was artificially evolved in rice cells using BEMGE through both Agrobacterium-mediated and particle-bom bardment-mediated transform ation.Four different types of amino acid substitutions in the evolved OsALS1,derived from two sites that have never been targeted by natural or human selection during rice dom estication,were identified,conferring varying levels of tolerance to the herbicide bispyribac-sodium.Furtherm ore,the P171F substitution identified in a strong OsALS1 allele was quickly introduced into the commercial rice cultivar Nangeng 46 through precise base editing w ith the corresponding base editor and sgRNA.Collectively,these data indicate great potential of BEMGE in creating important genetic variants of target genes for crop improvement. | Yongjie Kuang Shaofang Li Bin Ren Fang Yan Carl Spetz Xiangju Li Xueping Zhou Huanbin Zhou | 2020 | Molecular Plant2020，13，4： | 37 |
| 3 | A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice显示文摘Dear Editor,Gene-targeting technologies using sequence specific nucleases have been widely adopted to induce genetic modifications in both plant molecular biology research and crop improvement,of which generating targeted point mutation for gain-of-function phenotype is of great value.Although tremendous efforts have been made in developing geneinsertion or replacement approaches by | Bin Ren Fang Yan Yongjie Kuang Na Li Dawei Zhang Honghui Lin Huanbin Zhou | 2017 | Science China(Life Sciences)2017，60，5： | 28 |
| 4 | Highly Efficient A. T to G. C Base Editing by Cas9n- 3uided tRNA Adenosine Deaminase in Rice显示文摘 | Fang Yan Yongjie Kuang Bin Ren Jingwen Wang Dawei Zhang Honghui Lin Bing Yang Xueping Zhou Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 26 |
| 5 | ROS activates JNK-mediated autophagy to counteract apoptosis in mouse mesenchymal stem cells in vitro显示文摘目的: 为糖尿病的可勃起的机能障碍(编辑) 的治疗的间充质的干细胞(MSC ) 的移植被移植房间的 apoptosis 妨碍。在糖尿病的编辑,有增加的氧化应力并且在阴茎海绵体,和反应的氧种类(ROS ) 不减少导致移植房间的 apoptosis。在这研究,我们检验了是否并且 autophagy 怎么涉及 MSCs.Methods 的导致 ROS 的 apoptosis : 老鼠 C3H10 MSC 与 H 2 在糖尿病的编辑房间生存能力模仿高氧化的条件的 O 2 用 MTT 被测量试金。Apoptosis 被流动 cytometry 分析。Apoptosis 相关、 autophagy 相关的蛋白质与西方的污点试金被检测。细胞内部的 autophagosome 累积用传播电子 microscopy.Results 被学习: 有 H 2 O 2(50-400 μ mol/L ) 在集中依赖者和时间依赖者礼貌禁止了房间生存能力。而且, H 2 O 2(300 μ mol/L ) 在 MSC 的导致的 apoptosis,以及激活的 autophagy。有 lysosome 禁止者 chloroquine 的预告的处理(10 μ mol/L ) 或 PI3K 禁止者 3-methyladenine (5 mmol/L ) 显著地提高了 H 2 O 2-induced 房间死亡。有 JNK 禁止者 SP600125 的预告的处理(10 μ mol/L ) 废除 H 2 LC3-II 的 O 2-induced 累积,和稀释 H 2 在 MSCs.Conclusion 的 Bcl-2 层次的 O 2-induced 减小: ROS 导致 autophagy 由 JNK 的激活在 MSC 抵抗 apoptosis。因此, autophagy 的扩大可以减少 apoptosis,延长 MSC 幸存并且改进为糖尿病的编辑的基于 MSC 的治疗学的功效。 | Guan-yu LIU Xiao-xue JIANG Xin ZHU Wei-yang HE You-lin KUANG Ke REN Yong LIN Xin GOU | 2015 | Acta Pharmacologica Sinica2015，36，12： | 20 |
| 6 | Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice显示文摘CRISPR technologies enabling precise genome manipulation are valuable for gene function studies and molecular crop breeding. However, the requirement of a protospacer adjacent motif (PAM)y such as NGG and TTN, for Cas protein recognition restricts the selection of targetable genomic loci in practical applications of CRISPR technologies. Recently Cas9-NG, which recognizes a minimal NG PAM, was reported to expand the targeting space of genome editing in human cells, but it remains unclear whether this Cas9 variant can be used in plants. In this study, we evaluated the nuclease activity of Cas9-NG toward various NGN PAMs by targeting endogenous genes in transgenic rice. We found that Cas9-NG edits all NGG, NGA, NGT, and NGC sites with impaired activity, while the gene-edited plants were dominated by monoallelic mutations. Cas9-NG-engineered base editors were then developed and used to generate O s B Z R I gainof- function plants that can not be created by other available Cas9-engineered base editors. Moreover, we showed that a Cas9-NG-based transcriptional activator efficiently upregulated the expression of endogenous target genes in rice. In addition, we discovered that Cas9-NG recognizes NAC, NTG, NTT, and NCG apart from NG PAM. Together, these findings demonstrate that Cas9-NG can greatly expand the targeting scope of genome-editing tools, showing great potential for targeted genome editing, base editing, and genome regulation in plants. | Bin Ren Lang Liu Shaofang Li Yongjie Kuang Jingwen Wang Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2019 | Molecular Plant2019，12，7： | 18 |
| 7 | Preliminary study on the production of transgenic mice harboring hepatitis B virus X gene显示文摘INTRODUCTIONDespiteoverwhelmingepidemiologicalevidencelinkingpersistentHepatitisBVirus(HBV)infectionandthedevelopmentofhepato... | ZHU Huan Zhang 1, CHENG Guo Xiang 2, CHEN Jian Qu 2, KUANG Shu Yuan 3, CHENG Yong 2, ZHANG Xin Li 1, Ll Hou Da 2, XU Shao Fu 2, SHI Jing Quan 1, QIAN Geng Sun 3 and GU Jian Ren 3 | 1998 | World Journal of Gastroenterology1998，4，6： | 14 |
| 8 | Association between Ambient Air Pollution and Outpatient Visits for Acute Bronchitis in a Chinese City显示文摘Objective To investigate the short-term association between outdoor air pollution and outpatient visits for acute bronchitis,which is a rare subject of research in the mainland of China.Methods A time-series analysis was conducted to examine the association of outdoor air pollutants with hospital outpatient visits in Shanghai by using two-year daily data(2010-2011).Results Outdoor air pollution was found to be associated with an increased risk of outpatient visits for acute bronchitis in Shanghai.The effect estimates of air pollutants varied with the lag structures of the concentrations of the pollutants.For lag06,a 10 μg/m3 increase in the concentrations of PM10,SO2,and NO2 corresponded to 0.94%(95% CI:0.83%,1.05%),11.12%(95% CI:10.76%,11.48%),and 4.84%(95% CI:4.49%,5.18%) increases in hospital visits for acute bronchitis,respectively.These associations appeared to be stronger in females(P<0.05).Between-age differences were significant for SO2(P<0.05),and between-season differences were also significant for SO2(P<0.05).Conclusion Our analyses have provided the first evidence that the current air pollution level in China has an effect on acute bronchitis and that the rationale for further limiting air pollution levels in Shanghai should be strengthened. | GUO Li Juan ZHAO Ang CHEN Ren Jie KAN Hai Dong KUANG Xing Ya | 2014 | Biomedical and Environmental Sciences2014，27，11： | 13 |
| 9 | Ambient Temperature and Outpatient Visits for Acute Exacerbation of Chronic Bronchitis in Shanghai: A Time Series Analysis显示文摘The association between ambient temperature and acute exacerbation of chronic bronchitis(AECB)was still unknown.Therefore,we performed an epidemiological study in a large hospital of Shanghai to explore the relationship about temperature and outpatient visit for AECB.We adopted a quasi-Poisson generalized additive models and distributed lag nonlinear models to estimate the accumulative effects of | HUANG Fang ZHAO Ang CHEN Ren Jie KAN Hai Dong KUANG Xing Ya | 2015 | Biomedical and Environmental Sciences2015，28，1： | 7 |
| 10 | Frequent Gain and Loss of Resistance against Tobacco Mosaic Virus in Nicotiana Species显示文摘 | Yuan, Xinjie Yan, Chenghuan Wu, Zhujun Ren, Feihong Zhang, Hui Baker, Barbara Chen, Jiongjiong Kuang, Hanhui | 2015 | Molecular Plant2015，8，12： | 5 |
| 11 | A remote sensing monitoring method for alpine grasslands desertification in the eastern Qinghai–Tibetan Plateau显示文摘Alpine grassland is the typical vegetation in the eastern Qinghai–Tibetan Plateau,which has important ecological service functions,and also supports the development of alpine stock farming.In recent years,under both the natural and human disturbance,alpine grasslands in this area have appeared to different degrees of desertification.A diagnosis of the desertification degree serves as the basis for grassland ecological restoration.This study constructs a comprehensive index based on remote sensing called alpine grassland desertification index(AGDI)to monitor the areas and degree of desertification.The most relevant indicators of desertification,namely,vegetation fraction,aboveground biomass,soil moisture,and land surface temperature,were selected to establish AGDI.The geographical detector is used to reselect and assess these indicators.The results show that the overall verification accuracy of AGDI is 82.05%.In particular,the accuracy of identifying severe desertification is the highest.Our study confirms that the desertification of alpine grasslands in the eastern Qinghai–Tibetan Plateau is characterized by fragmentation.Thus,Landsat-8 OLI data with a spatial resolution of 30 m is more suitable than MODIS data for alpine grasslands desertification monitoring.The research results can provide a methodological reference for monitoring desertification of alpine grasslands and other grassland regions in the world. | KUANG Qian YUAN Quan-zhi HAN Ji-chong LENG Rong WANG Yu-shuang ZHU Ke-hong LIN Shuo REN Ping | 2020 | Journal of Mountain Science2020，17，6： | 4 |
| 12 | Overexpression CPT1A reduces lipid accumulation via PPARα/CD36 axis to suppress the cell proliferation in ccRCC显示文摘Clear cell renal carcinoma(ccRCC)is histologically defined by its cytoplasmic lipid deposits.Lipid metabolism disorder largely increases the risk of ccRCC.In this study,we aimed to investigate the biological functions and molecular mechanisms of carnitine palmitoyl transferase 1A(CPT1A)in ccRCC.Our results showed that CPT1A is decreased in ccRCC clinical samples and cell lines compared with that in normal samples.Lentivirus overexpressing CPT1A was used to investigate the neoplastic phenotypes of ccRCC,and the results showed that lipid accumulation and tumor growth are attenuated both in vitro and in vivo.In addition,CPT1A prevents cholesterol uptake and lipid accumulation by increasing the peroxisome proliferator-activated receptorα(PPARα)level through regulation of Class B scavenger receptor type 1(SRB1)and cluster of differentiation 36(CD36).Furthermore,PI3K/Akt signaling pathway promotes tumor cell proliferation in ccRCC,which is related to the enhanced expression of CD36.Functionally,weakened CPT1A expression is critical for lipid accumulation to promote ccRCC development.Collectively,our research unveiled a novel function of CPT1A in lipid metabolism via PPARα/CD36 axis,which provides a new theoretical explanation for the pathogenesis of ccRCC.Targeting CPT1A may be a potential therapeutic strategy to treat ccRCC. | Hui Yang Hongbo Zhao Zhongkun Ren Xiaojia Yi Qiao Zhang Zhe Yang Yingmin Kuang Yuechun Zhu | 2022 | Acta Biochimica et Biophysica Sinica2022，54，2： | 4 |
| 13 | Electron-withdrawing functional ligand promotes CO2 reduction catalysis in single atom catalyst显示文摘Electrochemical carbon dioxide reduction reaction (CO2RR) powered by renewable electricity offers an attractive approach to reduce carbon emission and at the same time produce valuable chemicals/fuels.To design efficient CO2 reduction electrocatalyst,it is important to understand the structure-activity relationship.Herein,we design a series of single Co atoms electrocatalysts with well-defined active sites electronic structures,which exhibit outstanding CO2RR activity with controllable selectivity to CO.Experimental and density functional theory (DFT) calculation studies show that introducing nitro (amino) ligand next to single Co atom catalytic center with electron-withdrawing (electron-donating) capability favors (hinders) CO2 reduction catalysis.This work provides an in-depth understanding of how functional ligand affects the splitting of transition metal 3d electron orbital,thereby changing the electron transfer from transition metal active site to CO2,which is closely related to the Gibbs free energy of the rate-determining step (CO2+e^-+*→*CO2^-). | Xinyi Ren Song Liu Huicong Li Jie Ding Linghui Liu Zhichong Kuang Ling Li Hongbin Yang Fuquan Bai Yanqiang Huang Tao Zhang Bin Liu | 2020 | Science China Chemistry2020，63，12： | 3 |
| 14 | Probiotics with anti-type 2 diabetes mellitus properties:targets of polysaccharides from traditional Chinese medicine显示文摘Traditional Chinese medicine polysaccharides is a biologically active ingredient that is not easy to be digested.It is fermented by intestinal microflora to promote qualitative and selective changes in the composition of the intestinal microbiome,which often result in beneficial effects on the health of the host.People call it“prebiotics”.In this review,we systematically summarized the anti-diabetic effect of traditional Chinese medicine polysaccharides.These polysaccharides regulate the metabolism of sugar and lipids by inter-influence with the intestinal microflora,and maintain human health,while improving type 2 diabetes-like symptoms such as high blood glucose,and abnormal glucose and lipid metabolism. | Lun WU Yue GAO Yang SU Jing LI Wen-Chen REN Qiu-Hong WANG Hai-Xue KUANG | 2022 | Chinese Journal of Natural Medicines2022，20，9： | 3 |
| 15 | Deficiency of Tfh Cells and Germinal Center in Deceased COVID-19 Patients显示文摘Summary:The COVID-19 pandemic caused by SARS-CoV2 is characterized by a remarkable variation in clinical severity ranging from a mild illness to a fatal multi-organ disease.Understanding the dysregulated human immune responses in the fatal subjects is critical for management of COVID-19 patients and the pandemic.In this study,we examined the immune cell compositions in the lung tissues and hilar lymph nodes using immunohistochemistry on 6 deceased COVID-19 patients and 4 focal organizing pneumonia(FOP)patients who underwent lung surgery and served as controls.We found a dominant presence of macrophages and a general deficiency of T cells and B cells in the lung tissues from deceased COVID-19 patients.In contrast to the FOP patients,Tfh cells and germinal center formation were largely absent in the draining hilar lymph nodes in the deceased COVID-19 patients.This was correlated with reduced IgM and IgG levels compared to convalescent COVID-19 patients.In summary,our data highlight a defect of germinal center structure in deceased COVID-19 patients leading to an impaired humoral immunity.Understanding the mechanisms of this deficiency will be one of the key points for the management of this epidemic. | Ya-qi DUAN Ming-hui XIA Liang REN Yan-fang ZHANG Qi-lin AO San-peng XU Dong KUANG Qian LIU Bing YAN Yi-wu ZHOU Qian CHU Liang LIU Xiang-Ping YANG Guo-ping WANG | 2020 | Current Medical Science2020，40，4： | 2 |
| 16 | Topotactically constructed nickel-iron(oxy)hydroxide with abundant in-situ produced high-valent iron species for efficient water oxidation显示文摘The low efficiency of oxygen evolution reaction(OER) is regarded as one of the major roadblocks for metal-air batteries and water electrolysis.Herein,a high-performance OER catalyst of NiFe_(0.2)(oxy)hydroxide(NiFe_(0.2)-O_(x)H_(y)) was developed through topotactic transformation of a Prussian blue analogue in an alkaline solution,which exhibits a low overpotential of only 263 mV to reach a current density of 10 mA cm^(-2) and a small Tafel slope of 35 mV dec-1.Ex-situ/operando Raman spectroscopy results indicated that the phase structure of NiFe_(0.2)-O_(x)H_(y) was irreversibly transformed from the type of α-Ni(OH)_(2) to γ-NiOOH with applying an anodic potential,while ex-situ/operando 57Fe Mossbauer spectroscopic studies evidenced the in-situ production of abundant high-valent iron species under OER conditions,which effectively promoted the OER catalysis.Our work elucidates that the amount of high-valent iron species in-situ produced in the NiFe(oxy)hydroxide has a positive correlation with its water oxidation reaction performance,which further deepens the understanding of the mechanism of NiFe-based electrocatalysts. | Zhichong Kuang Song Liu Xuning Li Meng Wang Xinyi Ren Jie Ding Rile Ge Wenhui Zhou Alexandre IRykov Moulay TSougrati Pierre-Emmanuel Lippens Yanqiang Huang Junhu Wang | 2021 | Journal of Energy Chemistry2021，30，6： | 2 |
| 17 | Recent diagnostic developments at the 100 kJ-level laser facility in China显示文摘A 100 kJ-level laser facility has been designed to study inertial confinement fusion physics in China.This facility incorporates various diagnostic techniques,including optical,x-ray imaging,x-ray spectrum,and fusion product diagnostics,as well as general diagnostics assistance systems and central control and data acquisition systems.This paper describes recent developments in diagnostics at the facility. | Feng Wang Shaoen Jiang Yongkun Ding Shenye Liu Jiamin Yang Sanwei Li Tianxuan Huang Zhurong Cao Zhenghua Yang Xin Hu Wenyong Miao Jiyan Zhang Zhebin Wang Guohong Yang Rongqing Yi Qi Tang Longyu Kuang Zhichao Li Dong Yang Yulong Li Xiaoshi Peng Kuan Ren Baohan Zhang | 2020 | Matter and Radiation at Extremes2020，5，3： | 2 |
| 18 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 19 | Measurements ofthe 197Au(n, γ) crosssectionupto100keV at the CSNSBack-nfacility显示文摘The neutron capture cross section of 197 Au was measured using the time-of-flight(TOF)technique at the Back-n facility of the China Spallation Neutron Source(CSNS)in the 1 eV to 100 keV range.Prompt c-rays originating from neutron-induced capture events were detected by four C_(6)D_(6) liquid scintillator detectors.Pulse height weighting technology(PHWT)was used to analyze the data.The results are in good agreement with ENDF/B-VIII.0,CENDL-3.1,and other evaluated libraries in the resonance region,and in agreement with both n TOF and GELINA experimental data in the 5–100 keV range.Finally,the resonance peaks in the energy range from 1eV to 1 keV were fitted by the SAMMY R-matrix code. | Xin-Rong Hu Gong-Tao Fan Wei Jiang Jie Ren Long-Xiang Liu Hong-Wei Wang Ying-Du Liu Xin-Xiang Li Yue Zhang Zi-Rui Hao Pan Kuang Xiao-He Wang Ji-Feng Hu Bing Jiang De-Xin Wang Suyalatu Zhang Zhen-Dong An Yu-Ting Wang Chun-Wang Ma Jian-Jun He Jun Su Li-Yong Zhang | 2021 | Nuclear Science and Techniques2021，32，9： | 2 |
| 20 | The general geometrical models of the design and 2 - axis NC machining of a helical end - mill with constant pitch显示文摘 | REN Bing - Yin TANG Yu - Yong CHEN Cha - Kuang | 2001 | Material Processing Technology2001，115，3： | 2 |
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