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| 1 | TLR2 and TLR4 polymorphisms influence m RNA and protein expression in colorectal cancer显示文摘AIM: To evaluate the effect of promoter region polymorphisms of toll-like receptor(TLR)2-196 to-174 del and TLR4-1607T/C(rs10759932) on m RNA and protein expression in tumor tissue and of TLR4+896A/G(rs4986790) on colorectal cancer(CRC) risk.METHODS: The TLR2-196 to-174 del polymorphism was investigated using allele-specific polymerase chain reaction(PCR) and the TLR4-1607T/C and TLR4+896A/G by PCR-restriction fragment length p o l y m o r p h i s m( R F L P). W e g e n o t y p e d 4 3 4 D N A samples from 194 CRC patients and 240 healthy individuals. The m RNA relative quantification(RQ) was performed in 40 tumor tissue samples by quantitative PCR Taq Man assay, using specific probes for TLR2 and TLR4 genes, and ACTB and GAPDH reference geneswere used as endogenous controls. Protein expression was analyzed by immunohistochemistry with specific primary antibodies.RESULTS: No association was found for TLR4-1607T/C and TLR4+896A/G by three statistical models(logadditive, dominant and recessive). However, based on dominant and log-additive models, the polymorphic variant TLR2-196 to-174 del was associated with increased CRC risk [dominant: odds ratio(OR) = 1.72, 95%CI: 1.03-2.89; P = 0.038 and log-additive: OR =1.59, 95%CI: 1.02-2.48; P = 0.039]. TLR2 m RNA expression was increased in tumor tissue(RQ = 2.36) when compared to adjacent normal tissue(RQ = 1; P < 0.0001), whereas the TLR4 m RNA showed a basal expression(RQ = 0.74 vs RQ = 1, P = 0.452). Immunohistochemistry analysis of TLR2 and TLR4 protein expression was concordant with the findings of m RNA expression. In addition, the TLR2-196 to-174 del variant carriers showed m RNA relative expression 2.19 times higher than wild-genotype carriers. The TLR2 protein expression was also higher for the TLR2-196 to-174 del variant carriers [117 ± 10 arbitrary unit(a.u.) vs 95 ± 4 a.u., P = 0.03]. However, for the TLR4-1607T/C polymorphism no significant difference was found for both m RNA(P = 0.56) and protein expression(P = 0.26).CONCLUSION: Our findings suggest that TLR2-196 to-174 del polymorphism increases TLR2 m RNA expression and is associated with higher CRC risk, indicating an important role in CRC genetic susceptibility. | Marcela Alcantara Proenca Juliana Garcia de Oliveira Aline Cristina Targa Cadamuro Maysa Succi Joao Gomes Netinho Eny Maria Goloni-Bertolo érika Cristina Pavarino Ana Elizabete Silva | 2015 | World Journal of Gastroenterology2015，21，25： | 9 |
| 2 | Artemisinin Biosynthesis in Non-glandular Trichome Cells of Artemisia annua显示文摘Artemisinin-based combination therapy(ACT)forms the first line of malaria treatment.However,the yield fluctuation of artemisinin has remained an unsolved problem in meeting the global demand for ACT.This problem is mainly caused by the glandular trichome(GT)-specific biosynthesis of artemisinin in all currently used Artemisia annua cultivars.Here,we report that non-GT cells of self-pollinated inbred A.annua plants can express the artemisinin biosynthetic pathway.Gene expression analysis demonstrated the transcription of six known pathway genes in GT-free leaves and calli of inbred A.annua plants.LC-qTOF-MS/MS analysis showed that these two types of GT-free materials produce artemisinin,artemisinic acid,and arteannuin B.Detailed IR-MALDESI image profiling revealed that these three metabolites and dihydroartemisinin are localized in non-GT cells of leaves of inbred A.annua plants.Moreover,we employed all the above approaches to examine artemisinin biosynthesis in the reported XL annua glandless(gl)mutant.The resulting data demonstrated that leaves of regenerated gl plantlets biosynthesize artemisinin.Codectively,these findings not only add new knowledge leading to a revision of the current dogma of artemisinin biosynthesis inannua but also may expedite innovation of novel metabolic engineering approaches for high and stable production of artemisinin in the future. | Rika Judd M.Caleb Bagley Mingzhuo Li Yue Zhu Caiyan Lei Seyit Yuzuak Mans Ekelof Gaobin Pu Xiting Zhao David C.Muddiman De-Yu Xie | 2019 | Molecular Plant2019，12，5： | 5 |
| 3 | Polaprezinc protects human colon cells from oxidative injury induced by hydrogen peroxide:Relevant to cytoprotective heat shock proteins显示文摘AIM: To investigate the effect of polaprezinc on cellular damage induced by hydrogen peroxide (H2O2) in human colon CaCo2 cells. METHODS: CaCo2 cells were treated with polaprezinc (10-100 μmol/L) for 6 h. After polaprezinc treatment, the cells were incubated with H2O2 (20 μmol/L) for 1 h. Cell viability was measured by MTT assay. Western blot analysis for heat shock protein (HSP) 27 and HSP72 in the cells was performed. Moreover, cells were pretreated with quercetin (200 μmol/L), an inhibitor of HSP synthe- sis, 2 h before polaprezinc treatment, and cell viability and the expression of HSP27 and 72 were assessed in these cells. RESULTS: Polaprezinc significantly protected CaCo2 cells from cell damage induced by H2O2, and up-regulated the expressions of HSP27 and HSP72 in the cells (10, 30 and 100 μmol/L of polaprezinc; 35.0% ± 7.7%, 58.3% ± 14.6% and 64.2% ± 8.2%, respectively. P < 0.01 versus polaprezinc-nontreated cells; 6.0% ± 4.4%). Quercetin inhibited the up-regulation of HSP27 and HSP72 by pola- prezinc and diminished the protective effect of polaprez- inc against H2O2-caused injury in the cells. CONCLUSION: Polaprezinc is a useful therapeutic agent for treatment of colitis and its effects depend on the function of cytoprotective HSP in colon. | Tatsuya Ohkawara Jun Nishihira Rika Nagashima Hiroshi Takeda Masahiro Asaka | 2006 | World Journal of Gastroenterology2006，12，38： | 3 |
| 4 | High K^+-induced contraction requires depolarization- induced Ca^2+ release from internal stores in rat gut smooth muscle显示文摘 | Timo KIRSCHSTEIN Mirko REHBERG Rika BAJORAT Tursonjan TOKAY Katrin PORATH Rudiger KOHLING | 2009 | Acta Pharmacologica Sinica2009，30，8： | 3 |
| 5 | Histological analysis of pollen-pistil interactions in sour passion fruit plants (Passiflora edulis Sims)显示文摘The success of sexual plant reproduction is directly influenced by specific interactions between the pollen and pistil.Light,fluorescence and scanning electron microscopy techniques were used to evaluate the steps of pollination in sour passion fruit plants(Passiflora edulis Sims).In the compatible interaction,pollen tubes grow through stigma projections towards the ovary.The pollen grain surface was found to be spheroidal and to consist of heteroreticulate exine with six colpi.Furthermore,analysis in vivo of pollenpistil interactions indicated that stigmas of flowers 24 hours before anthesis are unable to discriminate compatible(genetically unrelated)and incompatible(genetically related)pollen grains.Taken together,these results provide insight into the cellular mechanisms underlying pollination in passion fruit plants. | HÉRIKA CHAGAS MADUREIRA TELMA NAIR SANTANA PEREIRA MAURA DA CUNHA DENISE ESPELLET KLEIN | 2012 | BIOCELL2012，36，2： | 2 |
| 6 | Correlations of CTLA‐4 gene polymorphisms and hepatitis C chronic infection显示文摘 | Debora L. S.Danilovic Maria C.Mendes‐Correa érika U.Lima HevertonZambrini RaffaelleK. Barros SuemiMarui | 2012 | Liver Int2012，，5： | 2 |
| 7 | PERCIST用于头颈部鳞状细胞癌患者放化疗疗效评价及预后预测显示文摘目的探讨PET实体瘤疗效评价标准(PERCIST)评价头颈部鳞状细胞癌(HNSCC)患者放化疗疗效及预测复发和死亡的价值。方法42例患者(平均年龄63岁,范围20~79岁)在放化疗前及放化疗后约3个月(平均95d,范围70~119d)行脱氧葡萄糖(FDG)-PET/CT显像,其中包括10例鼻咽癌、13例口咽癌、11例下咽癌及8例喉癌。与PERCIST相关的无进展生存时间(PFS)和总生存时间(OS)采用对数秩检验和Cox回归分析进行统计学处理。结果PERCIST确定的完全代谢反应(CMR,即病灶FDG摄取降低至肝脏的平均活度水平以下或病灶FDG摄取与周围组织无明显差别)、部分代谢反应[PMR,即经去脂体质量校正的高峰标准摄取值(SULpeak)下降30%及以上]、稳定代谢反应(SMD,即FDG摄取无明显变化)和病灶代谢增加(PMD,即SULpeak增加30%及以上)的患者数分别为30例(71.4%)、9例(21.4%)、3例(7.1%)和0例。 | 唐军(译) Takayuki Katsuura Kazuhiro Kitajirma Masayuki Fujiwara Tomonori Terada Nobuhiro Uwa Kazuma Noguchi Hiroshi Doi Yukihisa Tamaki Rika Yoshida Tatsuya Tsuchitani Masahiro Fujita Koichiro Yamakado | 2018 | 中华核医学与分子影像杂志2018，38，12： | 2 |
| 8 | 无针头注射+皮内免疫--猪疫苗接种的一种完美组合方法显示文摘在养猪业中,接种疫苗是预防和控制多种病原体的首选方法。传统上,疫苗借助针头和注射器通过肌肉注射的方法进行接种。然而,鉴于注射方法对人类和动物健康的影响,由此激发了研究人员寻找替代性方法的研究。除了克服原有方法的缺点,替代方法的研制也是出于加强疗效的考虑,一些新方法的疗效与使用针头和注射器接种疫苗的方法一样甚至更为有效。本文介绍的这种方法结合了无针头注射和皮内接种疫苗各自的益处。 | 高凌云 潘雪男 韩浩月 Rika Jolie | 2016 | 国外畜牧学(猪与禽)2016，36，9： | 2 |
| 9 | Dual mode regulation of migration by lysophosphatidic acid in human gastric cancer cells显示文摘 | Dai Shida Joji Kitayama Hironori Yamaguchi Kotaro Hama Junken Aoki Hiroyuki Arai Hiroharu Yamashita Ken Mori Akihiro Sako Tsuyoshi Konishi Toshiaki Watanabe Teruyuki Sakai Rika Suzuki Hideo Ohta Yoh Takuwa Hirokazu Nagawa | 2004 | Experimental Cell Research2004，，2： | 2 |
| 10 | Room temperature ionic liquids of alkylimidazolium cations and fluoroanions显示文摘 | Rika Hagiwara Yasuhiko Ito | 2000 | Journal of Fluorine Chemistry2000，，2： | 2 |
| 11 | Development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for New World hantavirus infection显示文摘 | Takaaki Koma Kumiko Yoshimatsu Midori Taruishi Daisuke Miyashita Rika Endo Kenta Shimizu Shumpei P. Yasuda Takako Amada Takahiro Seto Ryo Murata Haruka Yoshida Hiroaki Kariwa Ikuo Takashima Jiro Arikawa | 2012 | Journal of Virological Methods2012，，1： | 2 |
| 12 | CD44 Variant Regulates Redox Status in Cancer Cells by Stabilizing the xCT Subunit of System xc ? and Thereby Promotes Tumor Growth显示文摘 | Takatsugu Ishimoto Osamu Nagano Toshifumi Yae Mayumi Tamada Takeshi Motohara Hiroko Oshima Masanobu Oshima Tatsuya Ikeda Rika Asaba Hideki Yagi Takashi Masuko Takatsune Shimizu Tomoki Ishikawa Kazuharu Kai Eri Takahashi Yu Imamura Yoshifumi Baba Mitsuyo O | 2011 | Cancer Cell2011，，3： | 2 |
| 13 | 显示文摘 | Rika Hagiwara Yasuhiko Ito | 2000 | Journal of Fluorine Chemistry2000，105，： | 1 |
| 14 | Effect of Chemical Composition on Recrystallization Behavior and r-Value in Ti-Added Ultra Low Carbon Sheet Steel显示文摘 | Yoda Rika Tsukatani Ichiro Inoue Tsuyoshi | 1994 | ISIJ International1994，34，1： | 1 |
| 15 | Direct electrolytic reduction of solid SiO2 in molten CaCl2 for the production of solar grade silicon显示文摘 | Kouji Y Toshiyuki N Rika H | 2007 | Electrochimica Acta2007，53，1： | 1 |
| 16 | Purification and characterization of an ε-poly-L-lysine-degrading enzyme from an ε-poly-L-lysine-producing strain of Streptomyces albulus显示文摘 | Mitsuaki Kito Rika Takimoto Toyokazu Yoshida Toru Nagasawa | 2002 | Archives of Microbiology2002，，5： | 1 |
| 17 | GATA transcription, translation and regulation in Haemaphysalis longicornis tick: Analysis of the cDNA and an essential role for vitellogenesis显示文摘 | Damdinsuren Boldbaatar Banzragch Battur Rika Umemiya-Shirafuji Min Liao Tetsuya Tanaka Kozo Fujisaki | 2010 | Insect Biochemistry and Molecular Biology2010，，1： | 1 |
| 18 | Room Temperature Ionic Liquids of Alkylimidazolium Cations and Fluoroanions显示文摘 | Rika Hagiwara Yasuhiko Ito | 2000 | Journal of Fluorine Chemistry2000，105，22： | 1 |
| 19 | Galectin-9 expression links to malignant potential of cervical squamous cell carcinoma显示文摘 | Meiyan Liang Masaki Ueno Souichi Oomizu Tomohiro Arikawa Rika Shinonaga Shulan Zhang Akira Yamauchi Mitsuomi Hirashima | 2008 | Journal of Cancer Research and Clinical Oncology2008，，8： | 1 |
| 20 | Determination of ammonia in low concentrations with Nessler's regent by flow injection analysis显示文摘 | Krug F.J Rika J Hansen E.H | | 0，，： | 1 |
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