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| 1 | A new cell death program regulated by toll-like receptor 9 through p38 mitogen-activated protein kinase signaling pathway in a neonatal rat model with sepsis associated encephalopathy显示文摘Background:Sepsis,a serious condition with high mortality,usually causes sepsis associated encephalopathy(SAE)that involves neuronal cell death.However,the cell death programs involved and their underlying mechanisms are not clear.This study aimed to explore the regulatory mechanisms of different cell death programs in SAE.Methods:A neonatal rat model of SAE was established by cecal ligation and perforation.Survival rate and vital signs(mean arterial pressure and heart rate)were monitored,nerve reflexes were evaluated,and cortical pathological changes were observed by hematoxylin and eosin staining.The expression of pyroptosis,apoptosis,and necroptosis(PANoptosis)-related proteins,mitogen-activated protein kinase(MAPK),and its upstream regulator toll-like receptor 9(TLR9)were detected.The expression of TLR9 in neurons was observed by immunofluorescence staining.The ultrastructure of neurons was observed by transmission electron microscope.Results:First,PANoptosis was found in cortical nerve cells of the SAE rats.Meanwhile,the subunits of MAPKs,p38 MAPK,Jun N-terminal kinase,and extracellular signal-regulated kinase(ERK)were activated.After pharmacologically inhibiting each of the subunits,only p38 MAPK was found to be associated with PANoptosis.Furthermore,blocking the p38 MAPK signaling pathway activated necroptosis but inhibited apoptosis and pyroptosis.When necroptosis was pharmacologically inhibited,apoptosis and pyroptosis were reactivated.Finally,we found that the expression of TLR9,a regulator of MAPKs,was significantly increased in this model.After down-regulation of TLR9,p38 MAPK,and ERK signaling pathways were inhibited,which led to the inhibition of PANoptosis.Further analysis found that down-regulation of TLR9 improved the survival rate and reduced the pathological changes in SAE rats.Conclusions:Our study showed that the programs comprising PANoptosis are activated simultaneously in SAE rats.TLR9 activated PANoptosis through the p38 MAPK signaling pathway.TLR9 may work as a potential target for SAE treatment. | Ruixi Zhou Junjie Ying Xia Qiu Luting Yu Yan Yue Qian Liu Jing Shi Xihong Li Yi Qu Dezhi Mu | 2022 | Chinese Medical Journal2022，，12： | 3 |
| 2 | The small GTPase RABA2a recruits SNARE proteins to regulate the secretory pathway in parallel with the exocyst complex in Arabidopsis显示文摘Delivery of proteins to the plasma membrane occurs via secretion,which requires tethering,docking,priming,and fusion of vesicles.In yeast and mammalian cells,an evolutionarily conserved RAB GTPase activation cascade functions together with the exocyst and SNARE proteins to coordinate vesicle transport with fusion at the plasma membrane.However,it is unclear whether this is the case in plants.In this study,we show that the small GTPase RABA2a recruits and interacts with the VAMP721/722-SYP121-SNAP33 SNARE ternary complex for membrane fusion.Through immunoprecipitation coupled with mass spectrometry analysis followed by the validatation with a series of biochemical assays,we identified the SNARE proteins VAMP721 and SYP121 as the interactors and downstream effectors of RABA2a.Further expreiments showed that RABA2a interacts with all members of the SNARE complex in its GTP-bound form and modulates the assembly of the VAMP721/722-SYP121-SNAP33 SNARE ternary complex.Intriguingly,we did not observe the interaction of the exocyst subunits with either RABA2a or theSNARE proteins in several different experiments.Neither RABA2a inactivation affects the subcellular localization or assembly of the exocystnor the exocyst subunit mutant exo84b shows the disrupted RABA2a-SNARE association or SNARE assembly,suggesting that the RABA2a-SNARE-and exocyst-mediated secretory pathways are largely independent.Consistently,our live imaging experiments reveal that the two sets of proteins follow non-overlapping trafficking routes,and genetic and cell biologyanalyses indicate that the two pathways select different cargos.Finally,we demonstrate that the plant-specific RABA2a-SNARE pathway is essential for the maintenance of potassium homeostasis in Arabisopsis seedlings.Collectively,our findings imply that higher plants might have generated different endomembrane sorting pathways during evolution and may enable the highly conserved endomembrane proteins to participate in plant-specific trafficking mechanisms for adaptation to the changing environment. | Lei Pang Zhiming Ma Xi Zhang Yuanzhi Huang Ruili Li Yansong Miao Ruixi Li | 2022 | Molecular Plant2022，15，3： | 2 |
| 3 | Timing of formation of the Hongdonggou Pb-Zn polymetallic ore deposit,Henan Province,China:Evidence from Rb-Sr isotopic dating of sphalerites显示文摘The Hongdonggou Pb-Zn polymetallic ore deposit,located in the southwestern part of the Luanchuan Mo-W-Pb-Zn-Ag polymetallic ore mineralization in Henan Province,China,is an important part of the East Qinling metallogenic belt.The orebodies in the deposit,which are vein,bedded and lenticular,are mainly hosted in the syenite porphyry,and formed within the carbonate and clastic rocks of the Yuku and Qiumugou formations partially.The genesis of the deposit has previously been argued to be of hydrothermal-vein type or of skarn-hydrothermal type.In this study,we report the results of Rb-Sr isotopic dating based on sphalerites from the main orebody of the Hongdonggou Pb-Zn polymetallic ore deposit,which yield an isochron age of 135.7 ± 3.2 Ma,constraining the timing of mineralization as early Cretaceous.The age is close to those reported for the Pb-Zn deposits in the Luanchuan ore belt.The(^(87)Sr/^(86)Sr),values of the sphalerites(0.71127 + 0.00010) are lower than that of terrigenous silicates(0.720) and higher than the mantle(0.707),suggesting that the metallogenic components were mainly derived through crust-mantle mixing.Combining the results from this study with those from previous work,we propose that the Hongdonggou Pb-Zn polymetallic ore deposit is a hydrothermal-vein deposit associated with the early Cretaceous tectonothermal event,and the mineralization is controlled by NWand near EW-trending faults in the Luanchuan Mo-W-Pb-Zn-Ag polymetallic ore concentration belt. | Fan Yang Gongwen Wang Huawen Cao Ruixi Li Li Tang Yufeng Huang Hao Zhang Fei Xue Wenjuan Jia Nana Guo | 2017 | Geoscience Frontiers2017，8，3： | 2 |
| 4 | An SVM-based machine learning method for accurate internet traffic classification显示文摘 | Ruixi Yuan Zhu Li Xiaohong Guan Li Xu | 2010 | Information Systems Frontiers2010，，2： | 1 |
| 5 | Preparation and characterization of macroporous poly (N-isopropylacrylamide) hydrogels for the controlled release of proteins显示文摘 | Zhuo Ruixi Li Wei | 2003 | J Polym Chem2003，4，： | 1 |
| 6 | Drop volume technique measuring interracial tension between two liquids显示文摘 | Zhang Ke Zongliang Du Ruixi Li | 2004 | Journal of Sichuan Universty (Engineering Science Edition)2004，36，2： | 1 |
| 7 | An SVM-based machine learning method for accurate Intemet traffic classification 显示文摘 | Yuan Ruixi Li Zhu Guan Xiaohong | 2010 | Information Systems Frontiers2010，12，2： | 1 |
| 8 | Resource prediction and assessment based on 3D/4D big data modeling and deep integration in key ore districts of North China显示文摘The North China district has been subjected to significant research with regard to the ore-forming dynamics,processes,and quantitative forecasting of gold deposits;it accounts for the highest number of gold reserves and annual products in China.Based on the top-level design of geoscience theory and the method adopted by the National Key R&D Project(deep process and metallogenic mechanism of North China Craton(NCC)metallogenic system),this paper systematically collects and constructs the geoscience data(district,camp,and deposit scales)in four key gold districts of North China(Jiaojia-Sanshandao,Southern Zhaoping,Wulong,and Qingchengzi).The settings associated with the geological dynamics of gold deposits were quantitatively and synthetically analyzed,namely:NCC destruction,metallogenic events,genetic models,and exploration models.Three-dimensional(3D)and four-dimensional(4D)geological modeling was performed using the big data on the districts,while the district-scale 3D exploration criteria were integrated to construct a quantitative exploration model.Among them,FLAC3D modelling and the Geo Cube software(version 3.0)were used to implement the numerical simulation of the 3D geological models and the constraints of the fluid saturation parameters of the Jiaojia fault to reconstruct the 4D fault structure models of the Jiaojia fault(with a depth of 5000 m).Using Geo Cube3.0,multiple integration modules(general weights of evidence(Wof E),Boost Wof E,Fuzzy Wof E,Logistic Regression,Information Entropy,and Random Forest)and exploration criteria were integrated,while the C-V fractal classification of A,B and C targets in four districts was carried out.The research results are summarized in the following four areas:(1)Four gold districts in the study area have more than three targets(the depth is 3000 m),and the class A,B and C targets exhibit a good spatial correlation with gold bodies that are controlled by mining engineering at depths greater than 1000 m.(2)The Boost Wof E method was used to identify the target optimization in 3D spaces(at depths of 3000–5000 m)of the Jiaojia-Sanshandao,Southern Zhaoping,and Wulong districts.(3)The general Wof E method is based on the Bayesian theory in 3D space and provides robust integration and target optimization that are suitable for the Jiaojia-Sanshandao and Southern Zhaoping districts in the Jiaodong area;it can also be applied to the Wulong district in the Liaodong area using a quantitative genetic model and an exploration model.Random forest is a multi-objective integration and target optimization method for 3D spaces,and it is suitable for the complex exploration model in the Qingchengzi district of the Liaodong area.The genetic model and exploration criteria associated with the exploration model of the Qingchengzi district were constrained by the common characteristics of the gold fault structure,magmatic rock emplacement in North China,and the strata fold and interlayer detachment structure.(4)Based on the gold reserves and the 3D block unit model of the Sanshandao gold deposit in the Jiaojia-Sanshandao district,the gold contents of the 3D block units in class A and B targets of the ore concentration were estimated to be 65.5%and 25.1%,respectively.The total Au resources of the optimized targets below a depth of 3000 m were 3908 t(including 1700 t reserves),and the total Au resources of the targets at depths from 3000 to 5000 m were 936 t.The study shows that the deep gold deposits in the four gold districts of North China exhibit a strong'transport-deposition'spatial correlation with potential targets.These'transport-deposition'spatial models represent the tectonic-magmatic-hydrothermal activities of the metallogenic system associated with the NCC destruction events and indicate the Au enrichment zones. | Gongwen WANG Zhiqiang ZHANG Ruixi LI Junjian LI Deming SHA Qingdong ZENG Zhenshan PANG Dapeng LI Leilei HUANG | 2021 | Science China Earth Sciences2021，64，9： | 1 |
| 9 | An SVM-based machine learning method for accurate internet traffic classification显示文摘 | Yuan Ruixi Li Zhu Guan Xiaohong | 2010 | Information Systems Frontiers2010，12，2： | 1 |
| 10 | An SVM-based machine learning method for accurate internet traffic classification显示文摘 | YUAN Ruixi LI Zhu GUAN Xiaohong Xu Li | 2010 | Information Systems Frontiers2010，12，2： | 1 |
| 11 | Modulating the C-terminus of DEP1 synergistically enhances grain quality and yield in rice显示文摘Grain shape is a key breeding target that influences both grain yield and appearance quality in rice. Breeding for long-slender grains is an effective strategy to improve grain quality (Jain et al., 2004;Wang et al., 2012, 2015a). In the past two decades, several quantitative trait loci (QTLs) responsible for grain size and shape have been identified, including GS3 (Fan et al., 2006), GS5 (Li et al., 2011). | Haixiang Huang Yafeng Ye Wenzhen Song Qi Li Ruixi Han Chenchen Wu Shuoxun Wang Jianping Yu Xueying Liu Xiangdong Fu Qian Liu Kun Wu | 2022 | Journal of Genetics and Genomics2022，49，5： | 1 |
| 12 | Broad-cast Ephemeris Accuracy Analysis for GPS Based on Precise Ephemeris 显示文摘 | Jia Ruixi Li Xiaoyu Xia Changfeng | 2014 | Applied Mechanics and Materials2014，602，： | 1 |
| 13 | An SVM-based machine learning method for accurate intemet traffic classification显示文摘 | Ruixi Yuan Zhu Li Xiaohong Guan | 2010 | Infor- marion Systems Frontiers2010，，： | 1 |
| 14 | MK-801 induces schizophrenic behaviors through downregulating Wnt signaling pathways in male mice显示文摘 | Jianfeng Yu Dake Qi Mengjuan Xing Ruixi Li Kaida Jiang Yuwen Peng Donghong Cui | 2011 | Brain Research2011，，： | 1 |
| 15 | Traffic classification- towards accurate real time network applications显示文摘 | Li Zhu Yuan Ruixi Guan Xiaohong | 2007 | Lecture Notes in Computer Science2007，4553，： | 1 |
| 16 | Abnormal anti-oxidation behavior of hexagonal boron nitride grown on copper显示文摘Atomic-layered hexagonal boron nitride(hBN)is expected to be the best two-dimensional(2D)anti-oxidation layer on metals for its incomparable impermeability,insulativity,and stability,as well as the progressive bottom-up growth techniques to ensure fast coating on metal surface in large area.However,its real anti-oxidation ability in practice is found to be unsatisfactory and nonuniform,and the main obstacle to achieving ideal anti-oxidation performance lies in unclear anti-oxidation behavior at special interface between 2D hBN and three-dimensional(3D)metals.Herein,system of monolayer hBN grown on copper(Cu)foils with various lattice orientations was grown to investigate the anti-oxidation behavior of different interlayer configurations.By using structural characterizations together with analysis of topography,we surprisingly found that stronger interlayer coupling led to worse anti-oxidation performance owing to fast diffusion of O2 through higher hBN corrugations generated at the commensurate hBN/Cu(111)configuration.In view of this,we developed the approach of cyclic reannealing that can effectively flatten corrugations and steps,and therefore improve the anti-oxidation performance to a great extent.This work provides a more indepth understanding of anti-oxidation behavior of 2D materials grown on 3D metals,and a practical method to pave the way for its large-scale applications in future. | Li Wang Jiajie Qi Shuai Zhang Mingchao Ding Wei Wei Jinhuan Wang Zhihong Zhang Ruixi Qiao Zhibin Zhang Zehui Li Kehai Liu Ying Fu Hao Hong Can Liu Muhong Wu Wenlong Wang Jun He Yi Cui Qunyang Li Xuedong Bai Kaihui Liu | 2022 | Nano Research2022，15，8： | 0 |
| 17 | D-π-D molecular layer electronically bridges the NiO_(x) hole transport layer and the perovskite layer towards high performance photovoltaics显示文摘Nickel oxide (NiO_(x)) has significant cost and stability advantages over poly[bis (4-phenyl)(2,4,6-trimethyl phenyl)amine](PTAA) for inverted p-i-n perovskite solar cells (PSCs),but the poor NiO_(x)/perovskite contact stemming from some reactive species at the interface led to suboptimal device performance.To solve this problem,we take a multiple donor molecule approach,using 3,3’-(4,8-bis(hexylthio)benzo[1,2-b:4,5-b’]dithiophene-2,6-diyl)bis(10-(6-bromohexyl)-10H-phenoxazine)(BDT-POZ) as an example,to modify the NiO_(x)/perovskite interface.The primary goal was to reduce the under-coordinated Ni^(≥3+) cations via electron transfer from the donor molecules to NiO_(x),thus mitigating the detrimental reactions between perovskite and NiO_(x).Equally importantly,the hole extraction at the interface was greatly enhanced after the organic donor modification,since the hydrophobic species atop NiO_(x) not only enabled pinhole-free crystallization of the perovskite but also properly tuned the interfacial energy level alignment.Consequently,the PSCs with NiO_(x)/BDT-POZ HTL achieved a high power conversion efficiency (PCE) up to 20.16%,which compared excellently with that of the non-modified devices (17.83%).This work provides a new strategy to tackle the exacting issues that have so far impeded the development of NiO_(x) based PSCs. | Rongguo Xu Xiuwen Xu Ruixi Luo Yu Li Gaopeng Wang Tongfa Liu Ning Cai Shihe Yang | 2022 | Journal of Energy Chemistry2022，31，4： | 0 |
| 18 | A distributed EEMDN-SABiGRU model on Spark for passenger hotspot prediction显示文摘To address the imbalance problem between supply and demand for taxis and passengers,this paper proposes a distributed ensemble empirical mode decomposition with normalization of spatial attention mechanism based bi-directional gated recurrent unit(EEMDN-SABiGRU)model on Spark for accurate passenger hotspot prediction.It focuses on reducing blind cruising costs,improving carrying efficiency,and maximizing incomes.Specifically,the EEMDN method is put forward to process the passenger hotspot data in the grid to solve the problems of non-smooth sequences and the degradation of prediction accuracy caused by excessive numerical differences,while dealing with the eigenmodal EMD.Next,a spatial attention mechanism is constructed to capture the characteristics of passenger hotspots in each grid,taking passenger boarding and alighting hotspots as weights and emphasizing the spatial regularity of passengers in the grid.Furthermore,the bi-directional GRU algorithm is merged to deal with the problem that GRU can obtain only the forward information but ignores the backward information,to improve the accuracy of feature extraction.Finally,the accurate prediction of passenger hotspots is achieved based on the EEMDN-SABiGRU model using real-world taxi GPS trajectory data in the Spark parallel computing framework.The experimental results demonstrate that based on the four datasets in the 00-grid,compared with LSTM,EMDLSTM,EEMD-LSTM,GRU,EMD-GRU,EEMD-GRU,EMDN-GRU,CNN,and BP,the mean absolute percentage error,mean absolute error,root mean square error,and maximum error values of EEMDN-SABiGRU decrease by at least 43.18%,44.91%,55.04%,and 39.33%,respectively. | Dawen XIA Jian GENG Ruixi HUANG Bingqi SHEN Yang HU Yantao LI Huaqing LI | 2023 | Frontiers of Information Technology & Electronic Engineering2023，24，9： | 0 |
| 19 | Effects of Shenlian formula(参连方)on microbiota and inflammatory cytokines in adults with type 2 diabetes:a double-blind randomized clinical trial显示文摘OBJECTIVES:To observe the efficacy of Shenlian formula(SL formula,参连方),which consist of Huanglian(Rhizoma Coptidis)and Renshen(Radix Ginseng),in the treatment of type 2 diabetes mellitus(T2DM)and explore the effects on gut microbiota and serum inflammatory cytokines.METHODS:In a double-blind,randomized,placebocontrolled parallel-group clinical trial,31 adults with T2DM were randomly allocated to receive the SL formula or placebo for 12 weeks.Body mass index(BMI),blood lipid indices,glycemic biomarkers including glycated hemoglobin(HbA1C),fasting plasma glucose(FPG),postprandial blood glucose(PBG),fasting insulin levels(FIL),fasting C-peptide(C-P),homoeostasis model assessment for insulin resistance(HOMA-IR)and inflammatory cytokines were assessed at baseline and 12 weeks.The contents of gut microbiota were determined by pyrosequencing of the V3-V4 regions of 16S rRNA genes.RESULTS:Sixteen cases were allocated in the treatment group and 15 in the placebo group.Compared with the placebo,SL formula resulted in a higher significant reduction in PBG[(-1.318±0.772)vs(-0.008±1.404)mmol/L,P=0.003],BMI[(-0.611±0.524)vs(0.957±2.212)kg/m2,P=0.01],FIL[(-1.627±6.268)vs(3.976±6.85)μIU/mL,P=0.02],HOMA-IR[(-0.530±1.461)vs(1.511±2.288),P=0.006],and C-reactive protein(CRP)[(-1.307±0.684)vs(0.828±0.557)mg/L,P=0.04].In terms of gut microbiota,compared with the placebo,the SL formula resulted in a significant decrease in species richness and evenness.CONCLUSIONS:The SL formula showed the efficacy to improve postprandial blood glucose,insulin resistance,BMI and CRP levels.In addition,it could reduce the total number,richness and evenness of species,meanwhile increase the abundance of probiotics to modulate the structure of gut microbiota in patients with T2DM.However,further studies are required for exploring the deeper mechanism of TCM on gut microbiota. | JIANG Li FU Qiang WANG Shidong ZHAO Jinxi CHEN Yu LI Jiayue XIAO Yonghua HUANG Weijun SUN Ruixi XIAO Yao SHEN Aijia WANG Junheng LIU Jiangteng FU Xiaozhe LI Yuanyuan ZHAO Yu XUE Taiqi | 2023 | Journal of Traditional Chinese Medicine2023，43，4： | 0 |
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