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| 1 | Aquaporin 5 is degraded by autophagy in diabetic submandibular gland显示文摘Autophagy is a catabolic process which is involved in the development of many diseases including diabetes mellitus and its complications. Hyposalivation is a common complication of diabetes mellitus, whereas its mechanism remains unclear. Here, we observed that the stimulated salivary flow rate of SMG was significantly decreased in db/db mice, a diabetic mice model. The expressions of aquaporin 5(AQP5), a water channel protein, were decreased, whereas the m RNA level of AQP5 was increased in SMGs of both diabetic patients and mice. Under transmission electron microcope, more autophagosomes were detected in diabetic SMGs. Expressions of autophagy related proteins LC3 II, Beclin-1 and ATG5 were increased, meanwhile autophagy substrate p62 was decreased in SMGs of diabetic patients and mice, indicating that autophagy was activated in diabetic SMG.Double immunofluorescence staining showed that the colocalization of AQP5 and LC3 was increased in SMGs of diabetic mice.In cultured SMG-C6 cells, high glucose(HG), but not high osmotic pressure, reduced AQP5 protein expression and induced autophagy. Moreover, inhibition of autophagy by 3-methyladenin, an autophagy inhibitor, or by autophagy-related gene 5 siRNA, decreased HG-induced AQP5 reduction in SMG-C6 cells. Additionally, the expression of p-p85, p-Akt and p-mTOR were decreased in HG-treated SMG-C6 cells. Pretreatment with 740 Y-P, a PI3 K agonist, significantly suppressed HG-induced autophagy and AQP5 degradation. Taken together, these results indicate that autophagy plays a crucial role in AQP5 degradation in diabetic SMG via PI3 K/Akt/mTOR signaling pathway, which contributes to the dysfunction of diabetic SMG. Our study provides a novel mechanism of diabetic hyposalivation. | Yan Huang Xijin Shi Qianying Mao Yan Zhang Xin Cong Xueming Zhang Zhejing Zhang Liling Wu Ruolan Xiang Guangyan Yu | 2018 | Science China(Life Sciences)2018，61，9： | 5 |
| 2 | Bacterial community dynamics during the traditional brewing ofWuyi Hong Qu glutinous rice wine as determined by culture-independent methods显示文摘 | LV XUCONG HUANG RUOLAN CHEN FANG | 2013 | Food Control2013，，34： | 1 |
| 3 | CCL2 recruitment of IL-6-producing CD11b^+ monocytes to the draining lymph nodes during the initiation of Th17-dependent B cell-mediated autoimmunity 显示文摘 | Yin Bai Ruolan Liu DeRen Huang | 2008 | EurJ Immunol2008，38，： | 1 |
| 4 | Treating COVID-19 with Chloroquine显示文摘A novel coronavirus disease 2019(COVID-19)emerged around December 2019 in Wuhan,China and has spread rapidly worldwide(Lu et al.,2020).Until March 27,2020,the Chinese health authorities had reported 82082 confirmed COVID-19 cases in China with 3298 deaths and 381443 confirmed cases with 20787 deaths outside China.The World Health Organization(WHO)named the virus SARS-CoV-2,which belongs to a distinct clade from the human severe acute respiratory syndrome CoV(SARS-CoV)and Middle East respiratory syndrome CoV(MERSCoV)(Zhu et al.,2020). | Mingxing Huang Tiantian Tang Pengfei Pang Man Li Ruolan Ma Jiahui Lu Jingxian Shu Yingying You Binghui Chen Jiabi Liang Zhongsi Hong Huili Chen Ling Kong Dajiang Qin Duanqing Pei Jinyu Xia Shanping jiang Hong Shan | 2020 | Journal of Molecular Cell Biology2020，12，4： | 1 |
| 5 | Bacterial community dynamics during the traditional brewing of Wuyi Hong Qu glutinous rice wine as determined by culture-independent methods 显示文摘 | Xucong Lv Ruolan Huang Fang Chen | 2013 | Food Control2013，34，： | 1 |
| 6 | Interactions between HMG proteins (HMG1/2 and HMG14/17) and human ε-globin gene promoter (ε-promoter)显示文摘High mobility group (HMG) proteins are abundant non-histone proteins in the nuclei of eukaryocytes. It has been shown that HMG proteins may play important roles in the structure and function of chromatin. In the present study, the binding of HMG proteins (HMG1/2 and HMG14/17) to the human e-globin gene promoter (e-promo-ter, -177-+1 bp) has been examined by using both the in vitro nucleosome reconstitution and the electrophoresis mobility shift assay (EMSA). We found that HMG1/2 proteins could bind to the naked e-promoter DNA, however, HMG14/17 could not. Using the in vitro nucleosome reconstitution, we revealed that HMG14/17 could bind to the mononucleosome reconstituted in vitro with E-promoter, while HMG1/2 could not. Those results indicate that the binding of HMG proteins to e-promoter is dynamic as the nucleosome assembling and disassembling. We speculated that this selective binding of HMG proteins to e-promoter might play a critical role in the regulation of e-globin gene expression. | Chunhui Hou Jian Huang Shubing Zhang Ruolan Qian | 2002 | Chinese Science Bulletin2002，47，18： | 0 |
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