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| 1 | 2019新型冠状病毒基因组特征和流行病学:病毒起源和受体结合的意义显示文摘研究者对来自9例新型冠状病毒肺炎住院患者的支气管肺泡灌洗液样本和培养的分离株进行了下一代测序。从这些个体中获得了严重急性呼吸综合征-冠状病毒2(severe acute respiratory syndrome-coronavirus 2,SARS-CoV-2)的完整和部分基因组序列。利用Sanger测序连接病毒重叠群以获得全长基因组,cDNA末端快速扩增确定终端区。对这些SARSCoV-2基因组和其他冠状病毒基因组进行了系统进化分析,以确定该病毒的进化史并有助于推断其可能的起源。 | 刘青(译) 刘莉(审校) Lu R Zhao X Li J Niu P Yang B Wu H Wang W Song H Huang B Zhu N Bi Y Ma X Zhan F Wang L Hu T Zhou H Hu Z Zhou W Zhao L Chen J Meng Y Wang J Lin Y Yuan J Xie Z Ma J Liu WJ Wang D Xu W Holmes EC Gao GF Wu G Chen W Shi W Tan W | 2020 | 中华高血压杂志2020，28，3： | 516 |
| 2 | Suppression of P-gp induced multiple drug resistance in a drug resistant gastric cancer cell line by overexpression of Fas显示文摘AIM To observe the drug sensitizing effect andrelated mechanisms of fas gene transduction onhuman drug-resistant gastric cancer cellSGC7901/VCR(resistant to Vincristine).METHODS The cell cycle alteration wasobserved by FACS.The sensitivity of gastriccancer cells to apoptosis was determined by invitro apoptosis assay.The drug sensitization ofcells to several anti-tumor drugs was observedby MTT assay.Immunochemical method wasused to show expression of P-gp and Topo Ⅱ ingastric cancer cells.RESULTS Comparing to SGC7901 and pBK-SGC7901/VCR,fas-SGC7901/VCR showeddecreasing G2 cells and increasing S cells,theG2 phase fraction of pBK-SGC7901/VCR wasabout 3.0 times that of fas-SGC7901/VCR,but Sphase fraction of fas-SGC7901/VCR was about1.9 times that of pBK-SGC7901/VCR,indicatingS phase arrest of fas-SGC7901/VCR.FACS alsosuggested apoptosis of fas-SGC7901/VCR,fas-SGC7901/VCR was more sensitive to apoptosisinducing agent VM-26 than pBK-SGC7901/VCR.MTT assay showed increased sensitization offas-SGC7901/VCR to DDP,MMC and 5-FU,butsame sensitization to VCR according to pBK-SGC7901/VCR.SGC7901,pBK-SGC7901/ VCRand fas-SGC7901/VCR had positively stainedTopo Ⅱ equally.P-gp staining in pBK- SGC7901/VCR was stronger than in SG07901,but there was little staining of P-gp in fas.SGC7901/VCR.CONCLUSION fas gene transduction couldreverse the MDR of human drug-resistant gastriccancer cell SGC7901/VCR to a degree,possiblybecause of higher sensitization to apoptosis anddecreased expression of P-gp. | Yin F Shi YQ Zhao WP Xiao B Miao JY Fan DM | 2000 | World Journal of Gastroenterology2000，6，5： | 24 |
| 3 | Structural basis of anti-PD-L1 monoclonal antibody avelumab for tumor therapy显示文摘 | Kefang Liu ShuguangTan Yan Chai Danqing Chen Hao Song Catherine Wei-Hong Zhang yi Shi Jun Liu Wenjie Tan Jianxin Lyu Shah Gao Jinghua Yan Jianxun Qi George F Gao | 2017 | Cell Research2017，27，1： | 11 |
| 4 | Structure and receptor-binding properties of an airborne transmissible avian infl uenza A virus hemagglutinin H5(VN1203mut)显示文摘Avian infl uenza A virus continues to pose a global threat with occasional H5N1 human infections,which is em-phasized by a recent severe human infection caused by avian-origin H7N9 in China.Luckily these viruses do not transmit effi ciently in human populations.With a few ami-no acid substitutions of the hemagglutinin H5 protein in the laboratory,two H5 mutants have been shown to obtain an air-borne transmission in a mammalian ferret model.Here in this study one of the mutant H5 proteins devel-oped by Kawaoka’s group(VN1203mut)was expressed in a baculovirus system and its receptor-binding properties were assessed.We herein show that the VN1203mut had a dramatically reduced binding affi nity for the avianα2,3-linkage receptor compared to wild type but showed no detectable increase in affi nity for the humanα2,6-linkage receptor,using Surface Plasmon Resonance techonology.Further,the crystal structures of the VN1203mut and its complexes with either human or avian receptors demon-strate that the VN1203mut binds the human receptor in the same binding manner(cis conformation)as seen for the HAs of previously reported 1957 and 1968 pandemic influenza viruses.Our receptor binding and crystallo-graphic data shown here further confi rm that the ability to bind the avian receptor has to decrease for a higher hu-man receptor binding affi nity.As the Q226L substitution is shown important for obtaining human receptor binding,we suspect that the newly emerged H7N9 binds human receptor as H7 has a Q226L substitution. | Xishan Lu Yi Shi Wei Zhang Yanfang Zhang Jianxun Qi George F Gao | 2013 | Protein & Cell2013，4，7： | 9 |
| 5 | Receptor activator of NF-κB Ligand (RANKL) expression is associated with epithelial to mesenchymal transition in human prostate cancer cells显示文摘 | Valerie A Odero-Marah Ruoxiang Wang Gina Chu Majd Zayzafoon Jianchun Xu Chunmeng Shi Fray F Marshall Haiyen E Zhau Leland WK Chung | 2008 | Cell Research2008，18，8： | 6 |
| 6 | Involvement of cholinergic system in suppression ot formalin-induced inflammatory pain by cobratoxin显示文摘瞄准: 调查 cobratoxin (CTX ) 的止痛效果,长链的 α从泰国眼镜蛇恶意的神经毒素,在导致福尔马林的煽动性的疼痛的一个老鼠模型。 | Gao-na SHI Yan-li LIU Hai-ming LIN Shi-lin YANG Yu-lin Feng Paul F REID Zheng-hong QIN | 2011 | Acta Pharmacologica Sinica2011，32，10： | 6 |
| 7 | The EST Analysis of A Suppressive Subtraction cDNA Library of Chinese Wild Vitis pseudoreticulata Inoculated with Uncinula necator显示文摘Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production. In order to get more informations on defense related EST sequences and help breeding program, we constructed and characterized a suppression subtractive hybridization cDNA library with artificially inoculated leaves and control Chinese wild Vitis pseudoreticulata clone Baihe-35-1, which is highly resistant to powdery mildew. In the library, the length of 58 EST fragments known as putative functions varied from 130 to 800 bp, and 60% of the ESTs exhibited high similarity to known sequences in database of GenBank with BLASTX analysis. These genes were involved in stress/defense response, detoxification, signal transduction, disease defense, and etc., and 14 ESTs remained unknown or hypothetical proteins, which may be new genes. The experiment provided an important basis for studying the disease-resistance mechanism and obtaining the genes for the aim of improving grapevine powdery mildew resistance. | SHI Jiang-li, WANG Yue-jin, ZHU Zi-guo and ZHANG Chao-hong Key Laboratory of Horticultural Plant Germplasm Utilization in Northwest China, Ministry of Agriculture/Shaanxi Key Laboratory of Molecular Biology for Agriculture/College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China | 2010 | Agricultural Sciences in China2010，9，2： | 5 |
| 8 | Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor显示文摘T 房间 receptor-CD3 建筑群(TCR ) 是能基于 CD3 细胞质的领域的各种各样的生物化学的变化开始抗原特定的有免疫力的回答的一台万用的发信号机器,但是内在的结构的基础留下逃犯。这里,我们开发了生物物理的途径学习 CD3 细胞质的领域的 conformational 动力学(CD3 CD ) 。在单个分子的水平,我们发现那 CD3 CD 能与三个功能的主题的不同坦诚有多重 conformational 状态,即, ITAM, BRS 和 PRS。因为,这些符合构造被产生 CD3 CD 有异构的类脂化合物绑定性质因此有异构的动力学。实时房间的成像实验证明不同抗原刺激能稳定 CD3 在不同符合构造的 CD 。类脂化合物依赖者 conformational 动力学因此为 TCR 的万用的发信号性质提供结构的基础。 | Xingdong Guo Chengsong Yan Hua Li Wenmao Huan Xiaoshan Shi Min Huang Yingfang Wang Weiling Pan Mingjun Cai Lunyi Li Wei Wu Yibing Bai Chi Zhang Zhijun Liu Xinyan Wang Xiaohui F Zhang Chun Tang Hongda Wang Wanli Liu Bo Ouyang Catherine C Wong Yi Cao Chenqi Xu | 2017 | Cell Research2017，27，4： | 5 |
| 9 | Inducing embryonic stem cells to differentiate into pancreatic beta cells by a novel three-step approach with activin A and all-trans retinoic acid显示文摘 | Shi Y Hou L Tang F Jiang W Wang P Ding M Deng H | 2006 | 中国生物学文摘2006，20，7： | 4 |
| 10 | MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors显示文摘 | Liu Xi Sempere Lorenzo F Ouyang Haoxu Memoli Vincent A Andrew Angeline S Luo Yue Demidenko Eugene Korc Murray Shi Wei Preis Meir Dragnev Konstantin H Li Hua DiRenzo James Bak Mads Freemantle Sarah J Kauppinen Sakari Dmitrovsky Ethan | 2010 | Journal of Clinical Investigation2010，，4： | 3 |
| 11 | Magnitude of modulation of gene expression in aneuploid maize depends on the extent of genomic imbalance显示文摘Aneuploidy has profound effects on an organism,typically more so than polyploidy,and the basis of this contrast is not fully understood.A dosage series of the maize long arm of chromosome 1(1L)was used to compa re relative global gene expression in diffe rent types and degrees of aneuploidy to gain insights into how the magnitude of genomic imbalance as well as hypoploidy affects global gene expression.While previously available methods require a selective examination of specific genes,RNA sequencing provides a whole-genome view of gene expression in aneuploids.Most studies of global aneuploidy effects have concentrated on individual types of aneuploids because multiple dose aneuploidies of the same genomic region are difficult to produce in most model genetic organisms.The genetic toolkit of maize allows the examination of multiple ploidies and 1-4 doses of chromosome arms.Thus,a detailed examination of expression changes both on the varied chromosome arms and elsewhere in the genome is possible,in both hypoploids and hyperploids,compared with euploid controls.Previous studies observed the inverse trans effect,in which genes not varied in DNA dosage were expressed in a negative relationship to the varied chromosomal region.This response was also the major type of changes found globally in this study.Many genes varied in dosage showed proportional expression changes,though some were seen to be partly or fully dosage compensated.It was also found that the effects of aneuploidy were progressive,with more severe aneuploids producing effects of greater magnitude. | Adam F·Johnson Jie Hou Hua Yang Xiaowen Shi Chen Chen Md Soliman Islam Tieming Ji Jianlin Cheng James A·Birchler | 2020 | Journal of Genetics and Genomics2020，47，2： | 3 |
| 12 | Interobserver agreement for contrast-enhanced ultrasound of liver imaging reporting and data system:A systematic review and metaanalysis显示文摘BACKGROUND Hepatocellular carcinoma is the most common primary liver malignancy.From the results of previous studies,Liver Imaging Reporting and Data System(LIRADS)on contrast-enhanced ultrasound(CEUS)has shown satisfactory diagnostic value.However,a unified conclusion on the interobserver stability of this innovative ultrasound imaging has not been determined.The present metaanalysis examined the interobserver agreement of CEUS LI-RADS to provide some reference for subsequent related research.AIM To evaluate the interobserver agreement of LI-RADS on CEUS and analyze the sources of heterogeneity between studies.METHODS Relevant papers on the subject of interobserver agreement on CEUS LI-RADS published before March 1,2020 in China and other countries were analyzed.The studies were filtered,and the diagnostic criteria were evaluated.The selected references were analyzed using the“meta”and“metafor”packages of R software version 3.6.2.RESULTS Eight studies were ultimately included in the present analysis.Meta-analysis results revealed that the summary Kappa value of included studies was 0.76[95%confidence interval,0.67-0.83],which shows substantial agreement.Higgins I2 statistics also confirmed the substantial heterogeneity(I2=91.30%,95%confidence interval,85.3%-94.9%,P<0.01).Meta-regression identified the variables,including the method of patient enrollment,method of consistency testing,and patient race,which explained the substantial study heterogeneity.CONCLUSION CEUS LI-RADS demonstrated overall substantial interobserver agreement,but heterogeneous results between studies were also obvious.Further clinical investigations should consider a modified recommendation about the experimental design. | Jun Li Ming Chen Zi-Jing Wang Shu-Gang Li Meng Jiang Long Shi Chun-Li Cao Tian Sang Xin-Wu Cui Christoph F Dietrich | 2020 | World Journal of Clinical Cases2020，8，22： | 2 |
| 13 | Modeling of chemical mechanical polishing with soft pads显示文摘 | Shi F G Zhao B | 1998 | Applied Physics A1998，67，： | 2 |
| 14 | Origin and diversity of novel avian influenza A H7N9 viruses causing human infection: phylogenetic, structural, and coalescent analyses显示文摘 | Di Liu Weifeng Shi Yi Shi Dayan Wang Haixia Xiao Wei Li Yuhai Bi Ying Wu Xianbin Li Jinghua Yan Wenjun Liu Guoping Zhao Weizhong Yang Yu Wang Juncai Ma Yuelong Shu Fumin Lei George F Gao | 2013 | The Lancet2013，，9881： | 2 |
| 15 | Adhesive joint design for high yield and low cost assembly of fiberoptic devices 显示文摘 | Lin Yaomin Liu Wenning Shi F G | 1992 | IEEE Circuits and Devices Magazine1992，8，6： | 2 |
| 16 | Response of antioxidative enzymes in cucumber chloroplasts to cadmium toxicity显示文摘 | Zhang F Q Shi W Y Jin Z X | 2003 | J Plant Nutr2003，26，： | 2 |
| 17 | Texture feature based on local fourier transform显示文摘 | Zhou F F Shi J Q | 2001 | IEEE International Conference on ImageProcessing2001，17，2： | 2 |
| 18 | Transfusion of autologous cytokine-induced killer cells inhibits viral replication in patients with chronic hepatitis B virus infection显示文摘 | Shi M Fu J Shi F | | Clinical Immunology0，，： | 2 |
| 19 | A DNA vector-based RNAi technology to suppress gene expression in mammalian cells显示文摘 | Sui G Soohoo C Affar el B Gay F Shi Y Forrester W C Shi Y | | 0，，： | 2 |
| 20 | Phycocyanin aggregation:A small angle neutron scattering and size exclusion chromatographic study显示文摘 | Shi Y Y Yun R H Van Gunsteren W F | 1988 | J Mol Biol1988，200，： | 1 |
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