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| 1 | Arsenic trioxide alleviates acute graft-versus-host disease by modulating macrophage polarization显示文摘This study aimed to explore macrophage polarization in acute graft-versus-host disease after hematopoietic stem cell transplantation, and investigated if arsenic trioxide(ATO) could correct this imbalance. In the colon of GVHD mice, we found that the number of F4/80+iNOS+ cells as well as the expression intensity of TNF-α and IL-1β was greater in the GVHD group than in the BM group, whereas the number of F4/80+CD206+ cells and the expression intensity of IL-10 and TGF-β was greater in the BM group than in the GVHD group. We investigated the effect of ATO on GVHD mice, and found that ATO treatment clearly improved the survival of the mice and reduced the severity of GVHD. In addition, ATO reduced the number of F4/80+iNOS+ cells, and increased the number of F4/80+CD206+ cells in the colon of GVHD mice. Furthermore, ATO sharply decreased CD86 and CD80 expression, and increased CD163 and CD206 expression in macrophages induced from aGVHD patients. Therefore,ATO can modulate the M1 and M2 phenotype in GVHD mice or in macrophages from aGVHD patients. Our data suggest that macrophage polarization is involved in the pathogenesis of aGVHD, and ATO treatment modulates macrophage polarization toward an M2 phenotype. | Xiao Liu Yan Su Xueyan Sun Haixia Fu Qiusha Huang Qi Chen Xiaodong Mo Meng Lv Yuan Kong Lanping Xu Xiaojun Huang Xiaohui Zhang | 2020 | Science China(Life Sciences)2020，63，11： | 6 |
| 2 | Mutant p53 increases exosome-mediated transfer of mi R-21-3p and mi R-769-3p to promote pulmonary metastasis显示文摘Objective: Tumor metastasis is a complex, multistep process that depends on tumor cells and their communication with the tumor microenvironment. A p53 gain-of-function mutant has been shown to enhance the tumorigenesis, invasion, and metastasis abilities of tumor cells. This study aimed to investigate the roles of p53 R273 H mutation in the tumor microenvironment.Methods: The in vitro and in vivo effects of the p53 R273 H mutant on the invasion and metastasis of HCT116 cells were investigated. Exosomes from wild-type and HCT116-TP53(R273 H) cells were cocultured with mouse embryonic fibroblasts(MEFs). The roles of differentially expressed exosomal micro RNAs identified by microarray analysis were investigated. The functions of the p53 R273 H mutant in tumor cells were also investigated via gene expression microarray and quantitative polymerase chain reaction(q PCR) analyses.Results: Introducing p53 R273 H mutant into HCT116 cells significantly potentiated pulmonary metastasis in vivo. In the presence of exosomes derived from HCT116-TP53(R273 H) cells, the exosomes were taken up by MEFs and became activated. Microarray analysis showed that the p53 R273 H mutation increased the exosomal levels of mi R-21-3 p and mi R-769-3 p. Intriguingly, in clinical samples, mi R-21-3 p and mi R-769-3 p levels were significantly higher in patients with a p53 mutation than in those without this mutation. Furthermore, both mi R-21-3 p and mi R-769-3 p activated fibroblasts and exerted a synergistic effect via their target genes on the transforming growth factor-β(TGF-β)/Smad signaling pathway. The activated fibroblasts excreted cytokine TGF-β and may have reciprocally induced cancer cells to undergo epithelial-mesenchymal transition(EMT). Indeed, HCT116-TP53(R273 H) cells showed increased expression of ZEB1 and SNAI2 and decreased transcription of several cell adhesion molecules.Conclusions: The mutant p53-exosomal mi R-21-3 p/mi R-769-3 p-fibroblast-cytokine circuit appears to be responsible for communication between tumor and stromal cells, with exosomal mi RNAs acting as a bridge. mi R-21-3 p and mi R-769-3 p are potential predictive markers of pulmonary metastasis and candidate targets for therapeutic interventions. | Qiang Ju Lina Zhao Jiajia Gao Lanping Zhou Yang Xu Yulin Sun Xiaohang Zhao | 2019 | Chinese Journal of Cancer Research2019，31，3： | 4 |
| 3 | Production of or-amylase by aspergillus oryzae as 3951in solid state fermentation using spent brewing grains as substrate 显示文摘 | Xu Hui Sun Lanping Zhao Daqing | 2008 | Journal of the Science of Food and Agriculture2008，88，3： | 1 |
| 4 | Two-phase anaerobic co-digestion of dairy manure with swine manure显示文摘In order to solve the problems associated with high fiber content,and the ensuing lower biogas volume yield in anaerobic digestion of dairy manure,a study of the co-digestion of separated liquids from dairy manure combined with swine manure using a two-phase anaerobic digestion process was conducted.The influence of level of total solids(TS)and hydraulic retention time(HRT)of the mixed liquor on the specific methane production were studied.Three TS levels 8%,10%and 12%were investigated.Analysis of the results show that a maximum specific methane yield of 132.99 L/kg volatile solids(VS),can be obtained with a TS of 9%,an inoculation rate of 30%,the duration of hydrolytic acidification phase of 5 d,and an HRT of the methanogenic phase of 10 d.These findings could provide directions for improving the biogas production by performing the co-digestion of dairy manure with swine manure. | Guan Zhengjun Sun Xianli Bi Lanping Li Wenzhe Zhang Ying Wang Zhigang | 2016 | International Journal of Agricultural and Biological Engineering2016，9，2： | 1 |
| 5 | Second unmanipulated allogeneic transplantation could be used as a salvage option for patients with relapsed acute leukemia post-chemotherapy plus modified donor lymphocyte infusion显示文摘Relapse is the main problem after allogeneic hematopoietic stem cell transplantation(allo-HSCT).The outcome of a second allo-HSCT(HSCT2)for relapse post-HSCT has shown promising results in some previous studies.However,little is known about the efficacy of HSCT2 in patients with relapsed/refractory acute leukemia(AL)post-chemotherapy plus modified donor lymphocyte infusion(post-Chemo+m-DLI)after the first allo-HSCT(HSCT1).Therefore,we retrospectively analyzed the efficacy of HSCT2 in 28 patients with relapsed/refractory AL post-Chemo+m-DLI in our center.With a median follow-up of 918(457–1732)days,26 patients(92.9%)achieved complete remission,and 2 patients exhibited persistent disease.The probabilities of overall survival(OS)and disease-free survival(DFS)1 year after HSCT2 were 25.0%and 21.4%,respectively.The cumulative incidences of nonrelapse mortality on day 100 and at 1 year post-HSCT2 were 7.1%±4.9%and 25.0%±8.4%.The cumulative incidences of relapse were 50.0%±9.8%and 53.5%±9.9%at 1 and 2 years post-HSCT2,respectively.Risk stratification prior to HSCT1 and percentage of blasts before HSCT2 were independent risk factors for OS post-HSCT2,and relapse within 6 months post-HSCT1 was an independent risk factor for DFS and relapse post-HSCT2.Our findings suggest that HSCT2 could be a salvage option for patients with relapsed AL post-Chemo+m-DLI. | Tingting Han Yuqian Sun Yang Liu Chenhua Yan Yu Wang Lanping Xu Kaiyan Liu Xiaojun Huang Xiaohui Zhang | 2021 | Frontiers of Medicine2021，15，5： | 1 |
| 6 | Visualizing the spatial distribution and alteration of metabolites in continuously cropped Salvia miltiorrhiza Bge using MALDI-MSI显示文摘Salvia miltiorrhiza Bge(SMB)has long been used in traditional Chinese medicine to treat cardiovascular and cerebrovascular diseases.Growing clinical usage has led to a huge demand for artificial planting of SMB.Thus,continuous cropping of SMB is an important challenge that needs to be addressed.Continuous cropping can alter the metabolic profile of plants,resulting in poor growth and low yield.In this study,we tried to image the spatial location and variation of endogenous metabolites in continuously cropped SMB using matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDIMSI).Spatially resolved expressions of tanshinones,salvianolic acids,polyamines,phenolic acids,amino acids,and oligosaccharides in normal and continuously cropped SMB roots were compared.The expressions of dihydrotanshinone Ⅰ,tanshinone Ⅱ A,dehydromiltirone,miltirone,dehydrotanshinone ⅡA,spermine,salvianolic acid B/E,tetrasaccharide,and pentasaccharide in continuously cropped SMB roots were much lower than those in normal roots.There was little difference in the expressions of caffeic acid and salvianolic acid A in normal and continuously cropped SMB roots.Ferulic acid was more widely distributed in xylem of normal SMB but strongly expressed in xylem,phloem,and cambium of continuously cropped SMB.The spatially resolved metabolite information enhances our understanding of the metabolic signature of continuously cropped SMB and also provides insights into the metabolic effects of continuous cropping in other plants. | Chenglong Sun Li Cui Bingqian Zhou Xiao Wang Lanping Guo Wei Liu | 2022 | Journal of Pharmaceutical Analysis2022，12，5： | 1 |
| 7 | Molecular monitoring and stepwise preemptive therapy for Epstein–Barr virus viremia after allogeneic stem cell transplantation显示文摘 | Qifa Liu Li Xuan Hui Liu Fen Huang Hongsheng Zhou Zhiping Fan Ke Zhao Meiqing Wu Lanping Xu Xiao Zhai Fuhua Zhang Can Liu Jing Sun Xiaojun Huang | 2013 | Am. J. Hematol2013，，7： | 1 |
| 8 | Optimization of cultivation conditions for extracellular polysaccharide and mycelium biomass by Morchella esculenta As51620显示文摘 | XU Hui SUN Lanping SHI Yazhong | 2008 | Biochemical Engineering Journal2008，39，1： | 1 |
| 9 | Association of C-reactive protein and metabolic syndrome in a rural Chinese population显示文摘 | Jiangping Wen Yuanbo Liang Fenghua Wang Lanping Sun Yongjun Guo Xinrong Duan Xiangyi Liu Qiushan Tao Tien Yin Wong Xinxin Lu Ningli Wang | 2009 | Clinical Biochemistry2009，，10： | 1 |
| 10 | Influence of the degree of donor bone marrow hyperplasia on patient clinical outcomes after allogeneic hematopoietic stem cell transplantation显示文摘This study evaluated the influence of the degree of donor bone marrow(BM)hyperplasia on patient clinical outcomes after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Twelve patients received allo-HSCT from hypoplastic BM donors between January 2010 and December 2017.Forty-eight patients whose donors demonstrated BM hyperplasia were selected using a propensity score matching method(1:4).Primary graft failure including poor graft function and graft rejection did not occur in two groups.In BM hypoplasia and hyperplasia groups,the cumulative incidence(CI)of neutrophil engraftment at day 28(91.7%vs.93.8%,P=0.75),platelet engraftment at day 150(83.3%vs.93.8%,P=0.48),the median time to myeloid engraftment(14 days vs.14 days,P=0.85)and platelet engraftment(14 days vs.14 days,P=0.85)were comparable.The 3-year progression-free survival,overall survival,CI of non-relapse mortality and relapse were 67.8%vs.71.7%(P=0.98),69.8%vs.77.8%(P=0.69),18.5%vs.13.6%(P=0.66),and 10.2%vs.10.4%(P=0.82),respectively.In multivariate analysis,donor BM hypoplasia did not affect patient clinical outcomes after allo-HSCT.If patients have no other suitable donor,a donor with BM hypoplasia can be used for patients receiving allo-HSCT if the donor Complete Blood Count and other examinations are normal. | Feifei Tang Xiaodong Mo Xiaohui Zhang Yu Wang Chenhua Yan Yuhong Chen Huan Chen Wei Han Yao Chen Fengrong Wang Jingzhi Wang Yuqian Sun Yuanyuan Zhang Kaiyan Liu Xiaojun Huang Lanping Xu | 2020 | Science China(Life Sciences)2020，63，1： | 0 |
| 11 | Theoretical investigation on the elusive biomimetic iron(Ⅲ)-iodosylarene chemistry: An unusual hydride transfer triggers the Ritter reaction显示文摘Introduction of iodosylarnes into biomimetic nonheme chemistry has made great achievement on identification of the subtle metal-oxygen reaction intermediates. However, after more than three decades of experimental and theoretical efforts the nature of the metal-iodosylarene adducts and the related dichotomous one-oxidant/multiple-oxident controversy have remained a matter of speculation. Herein, we report a theoretical study of the structure-activity relationship of the noted iron(Ⅲ)-iodsylarene complex,FeⅢ(Ph IO)(OTf)3(1), in oxygenation of cyclohexene. The calculated results revealed that 1 behaves like a chameleon by adapting its roles as a 2 e-oxidant or an oxygen donor, as a response to the regioselective attack of the C–H bond and the C=C bond. The oxidative C–H bond activation by 1 was found, for the first time, to proceed via a novel hydride transfer process to form a cyclohexene carbonium intermediate,such non-rebound step triggers the Ritter reaction to uptake an acetonitrile molecule to form the amide product, or proceeds with the rebound of the hydroxyl group return to the solvent cage to form the hydroxylated product. While in the C=C bond activation, 1 is a normal oxygen donor and shows two-state reactivity to present the epoxide product via a direct oxygen atom transfer mechanism. These mechanistic findings fit and explain the famous Valentine’s experiments and enrich the non-rebound scenario in bioinorganic chemistry. | Lanping Gao Xiaolu Chen Dongru Sun Hua Zhao Yufen Zhao Wonwoo Nam Yong Wang | 2021 | Chinese Chemical Letters2021，32，12： | 0 |
| 12 | Gut liver brain axis in diseases:the implications for therapeutic interventions显示文摘Gut-liver-brain axis is a three-way highway of information interaction system among the gastrointestinal tract,liver,and nervous systems.In the past few decades,breakthrough progress has been made in the gut liver brain axis,mainly through understanding its formation mechanism and increasing treatment strategies.In this review,we discuss various complex networks including barrier permeability,gut hormones,gut microbial metabolites,vagus nerve,neurotransmitters,immunity,brain toxic metabolites,β-amyloid(Aβ)metabolism,and epigenetic regulation in the gut-liver-brain axis.Some therapies containing antibiotics,probiotics,prebiotics,synbiotics,fecal microbiota transplantation(FMT),polyphenols,low FODMAP diet and nanotechnology application regulate the gut liver brain axis.Besides,some special treatments targeting gut-liver axis include farnesoid X receptor(FXR)agonists,takeda G protein-coupled receptor 5(TGR5)agonists,glucagon-like peptide-1(GLP-1)receptor antagonists and fibroblast growth factor 19(FGF19)analogs.Targeting gut-brain axis embraces cognitive behavioral therapy(CBT),antidepressants and tryptophan metabolism-related therapies.Targeting liver-brain axis contains epigenetic regulation and Aβmetabolism-related therapies.In the future,a better understanding of gut-liver-brain axis interactions will promote the development of novel preventative strategies and the discovery of precise therapeutic targets in multiple diseases. | Mengyao Yan Shuli Man Benyue Sun Long Ma Lanping Guo Luqi Huang Wenyuan Gao | 2024 | Signal Transduction and Targeted Therapy2024，9，1： | 0 |
| 13 | Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua L.显示文摘这研究瞄准位于 artemisinin 累积下面阐明的分子的机制由镉(Cd ) 导致了。Artemisia annua L 的生合成上的不同 Cd 集中(0, 20, 60,和 120 mol/L ) 的效果。被检验。中介和结束产品被 HPLC-ESI-MS/MS 分析确定。关键生合成酶的表示被 qRT-PCR 也决定。结果证明有为 3 天的 60 和 120 mol/L Cd 的处理的申请显著地改进了 artemisinic 酸, arteannuin B,和 artemisinin 的生合成。在 120 mol/L 的 artemisinic 酸, arteannuin B,和 artemisinin 的集中对待 Cd 的组分别地是比在控制组的那些高级 2.26, 102.08,和 33.63 倍的。在 60 mol/L 的 arteannuin B 和 artemisinin 的集中对待 Cd 的叶子分别地比在控制组的那些高 61.10 和 26.40 倍。HMGR, FPS,广告, CYP71AV1, DBR2, ALDH1,和 DXR 的相对表示层次在 120 mol/L 是起来调整的对待 Cd 的组因为在 3 天处理以后的 artemisinic 代谢物的增加的内容。因此, Cd 的适当剂量能增加在某个时间的代谢物指由的 artemisinic 的集中起来调整关键酶基因的相对表示层次在 artemisinin 包含了生合成。 | Liangyun Zhou Guang Yang Haifeng Sun Jinfu Tang Jian Yang Yizhan Wang Thomas Avery Garran Lanping Guo | 2017 | Frontiers of Medicine2017，11，1： | 0 |
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