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| 1 | Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells显示文摘Hydroxyapatite(HA)is a representative substance that induces bone regeneration.Our research team extracted nanohydroxyapatite(EH)from natural resources,especially equine bones,and developed it as a molecular biological tool.Polyethylenimine(PEI)was used to coat the EH to develop a gene carrier.To verify that PEI is well coated in the EH,we first observed the morphology and dispersity of PEI-coated EH(pEH)by electron microscopy.The pEH particles were well distributed,while only the EH particles were not distributed and aggregated.Then,the existence of nitrogen elements of PEI on the surface of the pEH was confirmed by EDS,calcium concentration measurement and fourier transform infrared spectroscopy(FT-IR).Additionally,the pEH was confirmed to have a more positive charge than the 25 kD PEI by comparing the zeta potentials.As a result of pGL3 transfection,pEH was better able to transport genes to cells than 25 kD PEI.After verification as a gene carrier for pEH,we induced osteogenic differentiation of DPSCs by loading the BMP-2 gene in pEH(BMP-2/pEH)and delivering it to the cells.As a result,it was confirmed that osteogenic differentiation was promoted by showing that the expression of osteopontin(OPN),osteocalcin(OCN),and runt-related transcription factor 2(RUNX2)was significantly increased in the group treated with BMP-2/pEH.In conclusion,we have not only developed a novel nonviral gene carrier that is better performing and less toxic than 25 kD PEI by modifying natural HA(the agricultural byproduct)but also proved that bone differentiation can be effectively promoted by delivering BMP-2 with pEH to stem cells. | Myung Chul Lee Hoon Seonwoo Kyoung Je Jang Shambhavi Pandey Jaewoon Lim Sangbae Park Jae Eun Kim Yun-Hoon Choung Pankaj Garg Jong Hoon Chung | 2021 | Bioactive Materials2021，6，9： | 2 |
| 2 | Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells显示文摘 | Jangho Kim Hong Nam Kim Ki-Taek Lim Yeonju Kim Shambhavi Pandey Pankaj Garg Yun-Hoon Choung Pill-Hoon Choung Kahp-Yang Suh Jong Hoon Chung | 2013 | Biomaterials2013，，30： | 1 |
| 3 | A novel digital algorithm for Sobel edge detection显示文摘 | Jagadish H P Shambhavi D S | 2010 | Communications in Computer and Information Science2010，70，： | 1 |
| 4 | Inflammatory Myofibroblastic Tumor of the Hepatobiliary System: Report of MR Imaging Appearance in Four Patients显示文摘 | Shambhavi V Richard CS Lona-Maria D | 2003 | Radiology2003，227，3： | 1 |
| 5 | Finite Element Analysis of sheet metal bending process to predict the springback显示文摘 | S.K. Panthi N. Ramakrishnan Meraj Ahmed Shambhavi S. Singh M.D. Goel | 2009 | Materials and Design2009，，2： | 1 |
| 6 | Inflammatory myofibroblastic tumor of the hepatobiliary system:report of MR imaging apperance in four patients显示文摘 | Shambhavi V Richard CS Iona-Maria D | | 0，，： | 1 |
| 7 | Inflam- matory Myofibroblastic Tumor of the Hepatobiliary System : Report of MR Imaging Appearance in Four Patients 显示文摘 | Shambhavi Venkataraman Semelka RC Larissa Braga | 2003 | Radiology2003，227，： | 1 |
| 8 | Inflammatory myofibroblastic tumor of the hepatobiliary systemr:report of MR imaging appearance in four patients显示文摘 | Shambhavi V Richard CS Larissa B | 2003 | Radiology2003，227，： | 1 |
| 9 | Inflammatory myofibro?blastic tumor of the hepatobiliary system: report of MR imaging appearance in four patients显示文摘 | Shambhavi V Bichant CS Hssa B | 2003 | Radiology2003，227，3： | 1 |
| 10 | Inflammatory myofihroblastic tumor of the hepatohiliary system report of MR imaging appearance in four patients 显示文摘 | Shambhavi V Richard CS Hssa B | 2003 | Radiology2003，227，： | 1 |
| 11 | A novel Digital Algorithm for Sobel Edge Detection显示文摘 | Jagadish P Shambhavi D S | 2010 | Communications in Computer and Information Science2010，70，6： | 1 |
| 12 | Medical image segmentation based on vigorous smoothingand edge detection ideology显示文摘 | Pujar Jagadish H Gurjal Pallavi S Shambhavi I) S | 2010 | International Journal of E-lectrical Computer Energetic and Computer Engineering2010，4，8： | 1 |
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