|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Observed displacement data-based identification method of deformation time-varying effect of high concrete dams显示文摘The long-term safety of high concrete dams has become the focus and central issues of public attention. Deformation is an actual comprehensive reflection of concrete dams. Especially the deformation time-varying effect, is a key index for evaluating the structural behavior, health status, and their evolution of a concrete dam in long-term service. In this paper, causing factors of the deformation time-varying effect of concrete dams were analyzed, and the time-varying effect was divided into two parts, which are the inherent time-varying effect and the time-varying effect caused by the change of dam structural performance. Then, based on the observed dam displacement and the wavelet multi-resolution analysis, causal models for identifying the later deformation time-varying effect and the identification process were proposed. Finally, the efficiency and rationality of the proposed method were verified by two actual concrete dams with runoff reservoir and regulatory reservoir. | WANG ShaoWei GU ChongShi BAO TengFei | 2018 | Science China(Technological Sciences)2018，61，6： | 4 |
| 2 | Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody显示文摘肝炎 E 病毒(HEV ) ,一个非包的、积极感觉的、搁浅单人赛的 RNA 病毒,是伤寒肝炎的一个主要原因。分类进家庭 Hepeviridae, HEV 包括四遗传型(遗传型 1-4 ) ,它属于单个 serotype。我们描述 monoclonal 抗体(mAb ) ,有 ~ 2.53-3.45 nM 的 8G12,同等地认出 HEV 的所有四遗传型,有约束力的亲密关系。mAb 8G12 有一保护,抵销能力,它能显著地在主人房间堵住病毒感染。动物与 1, 3 和 4 证实了跨遗传型的抵销的遗传型学习 8G12 和它肝炎 E 疾病的有效预防的能力。有丰富的遗传型的 HEV E2s 领域(病毒衣壳的最耸出的区域) 的 8G12 的复杂水晶结构 1 和 4 在 4.0 和 2.3 Å 被决定;分辨率分别地。这些结构表明 8G12 在 E2s dimerization 区域通过 epitopes 认出两遗传型。有像病毒的粒子的基于结构的 mutagenesis 和房间模型试金识别了为 8G12 中立化是必要的几保存残余(Glu549, Lys554 和 Gly591 ) 。而且, 8G12 的 epitope 作为涉及病毒主人相互作用的关键 epitope 被识别。这些调查结果将帮助为 HEV 感染的最丰富的形式的预防开发普通策略。 | Ying Gu Xuhua Tang Xiao Zhang Cuiling Song Minghua Zheng Kaihang Wang Jun Zhang Mun-Hon Ng Choy-Leong Hew Shaowei Li Ningshao Xia J. Sivaraman | 2015 | Cell Research2015，25，5： | 3 |
| 3 | Current progress in the development of prophylactic and therapeutic vaccines显示文摘Vaccines are essential public health tools and play an important role in reducing the burden of infectious diseases in the population.Emerging infectious diseases and outbreaks pose new challenges for vaccine development,requiring the rapid design and production of safe and effective vaccines against diseases with limited resources.Here,we focus on the development of vaccines in broad fields ranging from conventional prophylactic vaccines against infectious diseases to therapeutic vaccines against chronic diseases and cancer,providing a comprehensive overview of recent advances in eight different vaccine forms(live attenuated vaccines,inactivated vaccines,polysaccharide and polysaccharide conjugate vaccines,recombinant subunit vaccines,virus-like particle and nanoparticle vaccines,polypeptide vaccines,DNA vaccines,and m RNA vaccines)and the therapeutic vaccines against five solid tumors(lung cancer,breast cancer,colorectal cancer,liver cancer and gastric cancer),three infectious diseases(human immunodeficiency virus,hepatitis B virus and human papillomavirus-induced diseases)and three common chronic diseases(hypertension,diabetes mellitus and dyslipidemia).We aim to provide new insights into vaccine technologies,platforms,applications and understanding of potential next-generation preventive and therapeutic vaccine technologies,paving the way for the vaccines design in the future. | Tingting Li Ciying Qian Ying Gu Jun Zhang Shaowei Li Ningshao Xia | 2023 | Science China(Life Sciences)2023，66，4： | 1 |
| 4 | Truncated glycoprotein E of varicella-zoster virus is an ideal immunogen for Escherichia coli-based vaccine design显示文摘Varicella-zoster virus(VZV)is a highly infectious agent responsible for both varicella and herpes zoster disease.Despite high efficacy,there remain safety and accessibility concerns with the licensed vaccines.Here,we sought to produce a VZV g E immunogen using an E.coli expression system.We found that the soluble expression and yield of g E protein could be enhanced via C-terminal truncations to the protein,thereby facilitating a robust and scalable purification process for the purpose of vaccine manufacturing.The lead truncated g E(aa 31–358),hereafter referred to as tg E,was a homogenous monomer in solution and showed excellent antigenicity.Finally,we assessed and compared the immunogenicity of tg E with commercial v Oka LAV and Shingrix vaccine.We found that aluminum-adjuvanted tg E was immunogenic as compared with v Oka LAV.When adjuvanted with AS01B,a two-dose immunization of tg E showed comparable or better potency in antibody responses and cell-mediated immunity with those of the Shingrix vaccine at the same dosage,especially in terms of the proportion of IFN-γ-expressing CD4^(+)T cells.In conclusion,this method of E.coli-mediate tg E expression offers a cost-effective and scalable strategy to generate an ideal VZV g E immunogen for the development of both varicella and zoster vaccines. | Tingting Chen Jie Sun Sibo Zhang Tingting Li Liqin Liu Wenhui Xue Lizhi Zhou Siting Liang Zhili Yu Qingbing Zheng Hai Yu Tong Cheng Jun Zhang Ying Gu Shaowei Li Ningshao Xia | 2023 | Science China(Life Sciences)2023，66，4： | 1 |
| 5 | An Approach for Quantifying the Influence of Seepage Dissolution on Seismic Performance of Concrete Dams显示文摘Many concrete dams seriously suffer from long-term seepage dissolution,and the induced mechanical property deterioration of concrete may significantly affect the structural performance,especially the seismic safety.An approach is presented in this paper to quantify the influence of seepage dissolution on seismic performance of concrete dams.To connect laboratory test with numerical simulation,dissolution tests are conducted for concrete specimens and using the cumulative relative leached calcium as an aging index,a deterioration model is established to predict the mechanical property of leached concrete in the first step.A coupled seepage-calcium dissolutionmigrationmodel containing two calculation modes is proposed to simulate the spatially non-uniformdeterioration of concrete dams.Based on the simulated state of a roller compacted concrete dam subjected to 100 years of seepage dissolution,seismic responses of the damare subsequently analyzed.During which the nonlinear cracking of concrete,the radiation damping of the far-field foundation is considered.Research results show that seepage dissolution will seriously weaken the seismic safety of concrete dams because of the dissolution-induced decrease of effective thickness of the dam body.The upstream surface,dam toe and gallery wall suffer from a large degree of dissolution,whereas it is minimal and basically the same inside the dam body,at a degree of 0.19%within 100 years.The horizontal displacements of dam crest under the design static load and fortification against earthquake increase by 6.9%and 21.9%,respectively,and the dissolution-induced seismic cracking leads to the failure of dam anti-seepage system.This study can provide engineers with a reference basis for reinforcement decision of old concrete dams. | Shaowei Wang Cong Xu Hao Gu Pinghua Zhu Hui Liu Bo Xu | 2022 | Computer Modeling in Engineering & Sciences2022，，4： | 1 |
| 6 | 显示文摘 | Zhu Xiaonan Ni Shaowei | 2004 | The International Journal of Biochemistry & Cell Biology2004，36，： | 1 |
| 7 | Current progress and challenges in the design and development of a successful COVID-19 vaccine显示文摘The coronavirus disease 2019(COVID-19)pandemic,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is still a worldwide concern,with little to no sign of a decreasing trend.There is a general consensus that normal life will be hampered until a safe and effective vaccine strategy is available and globally administered.Numerous countries have accelerated the clinical trials process for the development of a successful COVID-19 treatment,with over 200 candidates presently available for testing against SARS-CoV-2.Here,we provide an overview of the COVID-19 vaccine candidates currently in development,discuss the scientific and practical challenges associated with COVID-19 vaccine design,and share the potential strategies that could be exploited for vaccine design success. | Tingting Li Tianying Zhang Ying Gu Shaowei Li Ningshao Xia | 2021 | Fundamental Research2021，1，2： | 1 |
| 8 | Sul-conraining Fluorinated Polyimides for Optical Waveguide Device显示文摘 | Wei Jiang Dong Wang Shaowei Gu qn et M | 2008 | Journal of Photochemistry and Photobiology A: Chemistry2008，，197： | 1 |
| 9 | Two antibodies show broad,synergistic neutralization against SARS-CoV-2 variants by inducing conformational change within the RBD显示文摘Continual evolution of the severe acute respiratory syndrome coronavirus(SARS-CoV-2)virus has allowed for its gradual evasion of neutralizing antibodies(nAbs)produced in response to natural infection or vaccination.The rapid nature of these changes has incited a need for the development of superior broad nAbs(bnAbs)and/or the rational design of an antibody cocktail that can protect against the mutated virus strain.Here,we report two angiotensin-converting enzyme 2 competing nAbs—8H12 and 3E2—with synergistic neutralization but evaded by some Omicron subvariants.Cryo-electron microscopy reveals the two nAbs synergistic neutralizing virus through a rigorous pairing permitted by rearrangement of the 472-489 loop in the receptor-binding domain to avoid steric clashing.Bispecific antibodies based on these two nAbs tremendously extend the neutralizing breadth and restore neutralization against recent variants including currently dominant XBB.1.5.Together,these findings expand our understanding of the potential strategies for the neutralization of SARS-CoV-2 variants toward the design of broad-acting antibody therapeutics and vaccines. | Hui Sun Tingting Deng Yali Zhang Yanling Lin Yanan Jiang Yichao Jiang Yang Huang Shuo Song Lingyan Cui Tingting Li Hualong Xiong Miaolin Lan Liqin Liu Yu Li Qianjiao Fang Kunyu Yu Wenling Jiang Lizhi Zhou Yuqiong Que Tianying Zhang Quan Yuan Tong Cheng Zheng Zhang Hai Yu Jun Zhang Wenxin Luo Shaowei Li Qingbing Zheng Ying Gu Ningshao Xia | 2024 | Protein & Cell2024，15，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员