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| 1 | Exercise-induced irisin in bone and systemic irisin administration reveal new regulatory mechanisms of bone metabolism显示文摘Irisin is a polypeptide hormone derived from the proteolytic cleavage of fibronectin-type III domaincontaining 5(FNDC5) protein. Once released to circulation upon exercise or cold exposure, irisin stimulates browning of white adipose tissue(WAT) and uncoupling protein 1(UCP1) expression, leading to an increase in total body energy expenditure by augmented UCP1-mediated thermogenesis. It is currently unknown whether irisin is secreted by bone upon exercise or whether it regulates bone metabolism in vivo. In this study, we found that 2 weeks of voluntary wheel-running exercise induced high levels of FNDC5 messenger RNA as well as FNDC5/irisin protein expression in murine bone tissues. Increased immunoreactivity due to exercise-induced FNDC5/irisin expression was detected in different regions of exercised femoral bones,including growth plate, trabecular bone, cortical bone, articular cartilage, and bone–tendon interface. Exercise also increased expression of osteogenic markers in bone and that of UCP1 in WAT, and led to bodyweight loss. Irisin intraperitoneal(IP) administration resulted in increased trabecular and cortical bone thickness and osteoblasts numbers, and concurrently induced UCP1 expression in subcutaneous WAT. Lentiviral FNDC5 IP administration increased cortical bone thickness. In vitro studies in bone cells revealed irisin increases osteoblastogenesis and mineralization, and inhibits receptor activator of nuclear factor-k B ligand(RANKL)-induced osteoclastogenesis. Taken together, our findings show that voluntary exercise increases irisin production in bone, and that an increase in circulating irisin levels enhances osteogenesis in mice. | Jin Zhang Paloma Valverde Xiaofang Zhu Dana Murray Yuwei Wu Liming Yu Hua Jiang Michel M Dard Jin Huang Zhiwei Xu Qisheng Tu Jake Chen | 2017 | Bone Research2017，5，1： | 23 |
| 2 | Characterization of Nestin-positive stem Leydig cells as a potential source for the treatment of testicular Leydig cell dysfunction显示文摘 | Mei Hua Jiang Bing Cai Ying Tuo Jiancheng Wang Zhi Jun Zang Xiang'an Tu Yong Gao Zhijian Su Weiqiang Li Guilan Li Min Zhang Jianwei Jiao Zi Wan Chunhua Deng Bruce T Lahn Andy Peng Xiang | 2014 | Cell Research2014，24，12： | 14 |
| 3 | From surface to bulk modification: Plasma polymerization of amine-bearing coating by synergic strategy of biomolecule grafting and nitric oxide loading显示文摘Integration of two or more biomolecules with synergetic and complementary effects on a material surface can help to obtain multi-functions for various biomedical applications.However,the amounts of biomolecules integrated and their physiological functions are compromised due to the limited surface anchoring sites.Herein,we propose a novel concept of film engineering strategy“from surface to bulk synergetic modification”.This new concept is realized by employing the surface amine groups of plasma polymerized allylamine(PPAm)film for grafting a molecule e.g.,thrombin inhibitor,bivalirudin(BVLD),meanwhile its bulk amine groups is used as a universal depot for storing and releasing therapeutic nitric oxide(NO)gas as supplement to the functions of BVLD.It is demonstrated that such a“from surface to bulk synergetic modification”film engineering can impart the modified-substrates with anti-platelet and anti-coagulant dual functions,giving rise to a highly endotheliummimetic thromboresistant property.We believe that our research provides a very promising strategy to deliver multifunctional surface versatilely that require NO release in combination with other properties,which will find broad biomedical applications in blood-contacting devices,and et al.Moreover,it also provides a brand-new film engineering strategy for tailoring surface multi-functionalities of a wide range of materials. | Tong Yang Zeyu Du Hua Qiu Peng Gao Xin Zhao Huaiyu Wang Qiufen Tu Kaiqin Xiong Nan Huang Zhilu Yang | 2020 | Bioactive Materials2020，5，1： | 10 |
| 4 | Endothelium-Mimicking Multifunctional Coating Modified Cardiovascular Stents via a Stepwise Metal-Catechol-(Amine)Surface Engineering Strategy显示文摘Stenting is currently the major therapeutic treatment for cardiovascular diseases.However,the nonbiogenic metal stents are inclined to trigger a cascade of cellular and molecular events including inflammatory response,thrombogenic reactions,smooth muscle cell hyperproliferation accompanied by the delayed arterial healing,and poor reendothelialization,thus leading to restenosis along with late stent thrombosis.To address prevalence critical problems,we present an endothelium-mimicking coating capable of rapid regeneration of a competently functioning new endothelial layer on stents through a stepwise metal(copper)-catechol-(amine)(MCA)surface chemistry strategy,leading to combinatorial endothelium-like functions with glutathione peroxidase-like catalytic activity and surface heparinization.Apart from the stable nitric oxide(NO)generating rate at the physiological level(2:2×10^(-10) mol/cm^(2)/min lasting for 60 days),this proposed strategy could also generate abundant amine groups for allowing a high heparin conjugation efficacy up to∼1μg/cm^(2),which is considerably higher than most of the conventional heparinized surfaces.The resultant coating could create an ideal microenvironment for bringing in enhanced antithrombogenicity,anti-inflammation,anti-proliferation of smooth muscle cells,re-endothelialization by regulating relevant gene expressions,hence preventing restenosis in vivo.We envision that the stepwise MCA coating strategy would facilitate the surface endothelium-mimicking engineering of vascular stents and be therefore helpful in the clinic to reduce complications associated with stenosis. | Ying Yang Peng Gao Juan Wang Qiufen Tu Long Bai Kaiqin Xiong Hua Qiu Xin Zhao Manfred FMaitz Huaiyu Wang Xiangyang Li Qiang Zhao Yin Xiao Nan Huang Zhilu Yang | 2020 | Research2020，，1： | 8 |
| 5 | OsSPL18 controls grain weight and grain number in rice显示文摘Grain weight and grain number are two important traits directly determining grain yield in rice. To date,a lot of genes related to grain weight and grain number have been identified; however, the regulatory mechanism underlying these genes remains largely unknown. In this study, we studied the biological function of OsSPL18 during grain and panicle development in rice. Knockout (KO) mutants of OsSPL18exhibited reduced grain width and thickness, panicle length and grain number, but increased tiller number. Cytological analysis showed that OsSPL18 regulates the development of spikelet hulls by affecting cell proliferation. qRT-PCR and GUS staining analyses showed that OsSPL18 was highly expressed in developing young panicles and young spikelet hulls, in agreement with its function in regulating grain and panicle development. Transcriptional activation experiments indicated that OsSPL18is a functional transcription factor with activation domains in both the N-terminus and C-terminus, and both activation domains are indispensable for its biological functions. Quantitative expression analysis showed that DEP1, a major grain number regulator, was significantly down-regulated in OsSPL18 KO lines.Both yeast one-hybrid and dual-luciferase (LUC) assays showed that OsSPL18 could bind to the DEP1promoter, suggesting that OsSPL18 regulates panicle development by positively regulating the expression of DEP1. Sequence analysis showed that OsSPL18 contains the OsmiR156k complementary sequence in the third exon; 5?RLM-RACE experiments indicated that OsSPL18 could be cleaved by OsmiR156k. Taken together, our results uncovered a new OsmiR156k-OsSPL18-DEP1 pathway regulating grain number in rice. | Hua Yuan Peng Qin Li Hu Shijie Zhan Shifu Wang Peng Gao Jing Li Mengya Jin Zhengyan Xu Qiang Gao Anping Du Bin Tu Weilan Chen Bingtian Ma Yuping Wang Shigui Li | 2019 | Journal of Genetics and Genomics2019，46，1： | 8 |
| 6 | Loss of Gn1a/OsCKX2 confers heavy-panicle rice with excellent lodging resistance显示文摘Significant achievements have been made in breeding programs for the heavy-panicle-type(HPT)rice(Oryza sativa) in Southwest China. The HPT varieties now exhibit excellent lodging resistance,allowing them to overcome the greater pressures caused by heavy panicles. However, the genetic mechanism of this lodging resistance remains elusive. Here, we isolated a major quantitative trait locus, Panicle Neck Diameter 1(PND1), andidentified the causal gene as GRAIN NUMBER 1 A/CYTOKININ OXIDASE 2(Gn1 A/Os CKX2). The null gn1 a allele from rice line R498(gn1 aR498) improved lodging resistance through increasing the culm diameter and promoting crown root development.Loss-of-function of Gn1 a/Os CKX2 led to cytokinin accumulation in the crown root tip and accelerated the development of adventitious roots. Gene pyramiding between the null gn1 aR498 allele with two gain-of-function alleles, STRONG CULM 2(SCM2)and SCM3, further improved lodging resistance.Moreover, Gn1 a/Os CKX2 had minimal influence on overall rice quality. Our research thus highlights the distinct genetic components of lodging resistance of HPT varieties and provides a strategy for tailormade crop improvement of both yield and lodging resistance in rice. | Bin Tu Zhang Tao Shiguang Wang Lei Zhou Ling Zheng Chun Zhang Xinzi Li Xiaoyu Zhang Junjie Yin Xiaobo Zhu Hua Yuan Ting Li Weilan Chen Peng Qin Bingtian Ma Yuping Wang Shigui Li | 2022 | Journal of Integrative Plant Biology2022，64，1： | 5 |
| 7 | Early Kinetics of Procalcitonin in Predicting Surgical Outcomes in Type A Aortic Dissection Patients显示文摘 | Hua Liu Zhe Luo Lan Liu Xiao-Mei Yang Ya-Min Zhuang Ying Zhang Guo-Wei Tu Guo-Guang Ma Guang-Wei Hao Jian-Feng LUO Ji-Li Zheng Chun-Sheng Wang | 2017 | Chinese Medical Journal2017，，10： | 5 |
| 8 | Breaking mechanism and control technology of sandstone straight roof in thin bedrock stope显示文摘The key problem to be solved urgently is how to avoid the occurrence of support break-off and water inrush in the stoping of sandstone straight roof under the action of load transfer in unconsolidated aquifer.For this reason,taking the thin bedrock 1602(3)working face of Huainan(the middle part of Anhui Province)Panyi Coal Mine as the engineering background,this study establishes the stope mining model by using the discrete element UDEC software and the mathematics mechanical model of the support load,and analyzes the reason of support crushing and decides to re-mining the working face by using the compulsive roof caving method.It is concluded that when the working face of sandstone straight roof is broken,the'voussoir beam'structure cannot be formed and acts on the support in the form of cantilever beam,but only when it falls to the high key stratum can the'voussoir beam'structure be formed and at this point,at this time,the bracket bears the weight of the rock layer in the range from the fractured sandstone layer to the lower critical layer.The working resistance of the support increases with the increase of the thickness and the breaking length of straight sandstone roof.When the breaking length of the roof reaches a certain extreme value,the support crushing accidents will occur.Managing roof with compulsive roof caving method can reduce the intensity of rock pressure in the stope,and the working face can be safely stoped,which provides a certain reference for similar conditions. | Hualei Zhang Min Tu Hua Cheng Yongzhi Tang | 2020 | International Journal of Mining Science and Technology2020，30，2： | 4 |
| 9 | Resting-state network complexity and magnitude changes in neonates with severe hypoxic ischemic encephalopathy显示文摘Resting-state functional magnetic resonance imaging has revealed disrupted brain network connectivity in adults and teenagers with cerebral palsy. However, the specific brain networks implicated in neonatal cases remain poorly understood. In this study, we recruited 14 termborn infants with mild hypoxic ischemic encephalopathy and 14 term-born infants with severe hypoxic ischemic encephalopathy from Changzhou Children's Hospital, China. Resting-state functional magnetic resonance imaging data showed efficient small-world organization in whole-brain networks in both the mild and severe hypoxic ischemic encephalopathy groups. However, compared with the mild hypoxic ischemic encephalopathy group, the severe hypoxic ischemic encephalopathy group exhibited decreased local efficiency and a low clustering coefficient. The distribution of hub regions in the functional networks had fewer nodes in the severe hypoxic ischemic encephalopathy group compared with the mild hypoxic ischemic encephalopathy group. Moreover, nodal efficiency was reduced in the left rolandic operculum, left supramarginal gyrus, bilateral superior temporal gyrus, and right middle temporal gyrus. These results suggest that the topological structure of the resting state functional network in children with severe hypoxic ischemic encephalopathy is clearly distinct from that in children with mild hypoxic ischemic encephalopathy, and may be associated with impaired language, motion, and cognition. These data indicate that it may be possible to make early predictions regarding brain development in children with severe hypoxic ischemic encephalopathy, enabling early interventions targeting brain function. This study was approved by the Regional Ethics Review Boards of the Changzhou Children's Hospital(approval No. 2013-001) on January 31, 2013. Informed consent was obtained from the family members of the children. The trial was registered with the Chinese Clinical Trial Registry(registration number: ChiCTR1800016409) and the protocol version is 1.0. | Hong-Xin Li Min Yu Ai-Bin Zheng Qin-Fen Zhang Guo-Wei Hua Wen-Juan Tu Li-Chi Zhang | 2019 | Neural Regeneration Research2019，14，4： | 4 |
| 10 | Nitric oxide-generating compound and bio-clickable peptide mimic for synergistically tailoring surface anti-thrombogenic and anti-microbial dual-functions显示文摘Application of extracorporeal circuits and indwelling medical devices has saved many lives.However,it is accompanied with two major complications:thrombosis and infection.To address this issue,we apply therapeutic nitric oxide gas(NO)and antibacterial peptide for synergistically tailoring such devices for surface anti-thrombogenic and antifouling dual functions.Such functional surface is realized by stepwise conjugation of NO-generating compound of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid(DOTA)chelated copper ions(Cu-DOTA)and dibenzylcyclooctyne-(DBCO-)modified antimicrobial peptide based on carbodiimide and click chemistry respectively.The integration of peptide and Cu-DOTA grants the modified surface the ability to not only efficiently inhibit bacterial growth,but also catalytically generate NO from endogenous s-nitrosothiols(RSNO)to reduce adhesion and activation of platelets,preventing the formation of thrombus.We envision that the stepwise synergistic modification strategy by using anticoagulant NO and antibacterial peptide would facilitate the surface multifunctional engineering of extracorporeal circuits and indwelling medical devices,with reduced clinical complications associated with thrombosis and infection. | Han Yu Shaoxing Yu Hua Qiu Peng Gao Yingzhong Chen Xin Zhao Qiufen Tu Minggang Zhou Lin Cai Nan Huang Kaiqin Xiong Zhilu Yang | 2021 | Bioactive Materials2021，6，6： | 3 |
| 11 | A single nucleotide substitution at 5'-UTR of GSN1 represses its translation and leads to an increase of grain length in rice显示文摘Rice grain size is an important trait that affects rice yield and quality, and thus the identification of genes related to grain size is of great significance for improving rice yield and quality. Many genes related to grain size, such as DEP1(Huang et al., 2009),GW5(Liu et al., 2017). | Xia Zhang Peng Qin Youlin Peng Bo Ma Jiangbo Hu Shijun Fan Binhua Hu Guohua Zhang Hua Yuan Wei Yan Weilan Chen Bin Tu Hang He Bingtian Ma Yuping Wang Shigui Li | 2019 | Journal of Genetics and Genomics2019，46，2： | 2 |
| 12 | Synthesis and Crystal Structure of 1,7,7-Trimethyl-4-(4-methoxyphenyl)-4,6,7,8-tetrahydroquinoline-2,5(1H,3H)-diones under Microwave Irradiation显示文摘 | HUA Guo-Ping ZHU Xiao-Tong ZHANG Jin-Peng XU Jia-Ning WANG Qian JI Shun-Jun ZHANG Yong TU Shu-Jiang | 2006 | Chinese Journal of Structural Chemistry2006，25，5： | 2 |
| 13 | An analytical model for heat and mass transfer processes in internally cooled or heated liquid desiccant–air contact units显示文摘 | Cheng Qin Ren Min Tu Hua Hui Wang | 2007 | International Journal of Heat and Mass Transfer2007，，17： | 2 |
| 14 | Isolation and Characterization of Recombinant Variable Domain of Heavy Chain Anti-idiotypic Antibodies Specific to Aflatoxin B_1显示文摘Some unique subclasses of Camelidae antibodies are devoid of the light chain,and the antigen binding site is comprised exclusively of the variable domain of the heavy chain(VHH).The recombinant VHHs have a high potential as alternative reagents for the next generation of immunoassay.In particular,they might be very useful for molecular mimicry.The present study demonstrated an alpaca immunized with the F(ab’)2fragment of anti-aflatoxin B1 mAb and developed an important anti-idiotypic(anti-Id)responses.Antigen-specific elution method was used for panning private anti-Id VHHs from the constructed alpaca VHH library.The selected VHHs were expressed,renatured,purified,and then identified by a competitive enzyme-linked immunosorbent assay(ELISA).Our findings indicated that the VHH would be an alternative tool for haptens mimicry studies. | WANG Dan XU Yang TU Zhui FU Jin Heng XIONG Yong Hua FENG Fan TAO Yong LEI Da | 2014 | Biomedical and Environmental Sciences2014，27，2： | 2 |
| 15 | A natural allele of TAW1 contributes to high grain number and grain yield in rice显示文摘Grain number per panicle (GNP) is a complex trait controlled by quantitative trait loci (QTL),directly determining grain yield in rice.Identifying GNP-associated QTL is desirable for increasing rice yield.A rice chromosome segment substitution line (CSSL),F771,which showed increased panicle length and GNP,was identified in a set of CSSLs derived from a cross between two indica cultivars,R498 (recipient) and WY11327 (donor).Genetic analysis showed that the panicle traits in F771 were semidominant and controlled by multiple QTL.Six QTL were consistently identified by QTL-seq analysis.Among them,the major QTL q PLN10 for panicle length and GNP was localized to a 121-kb interval between markers N802 and N909 on chromosome 10.Based on quantitative real-time PCR and sequence analysis,TAWAWA1(TAW1),a known regulator of rice inflorescence architecture,was identified as the candidate gene for q PLN10.A near-isogenic line,NIL-TAW1,was developed to evaluate its effects.In comparison with the recurrent parent R498,NIL-TAW1 showed increased panicle length (14.0%),number of secondary branches (20.9%) and GNP (22.0%),and the final grain yield per plant of NIL-TAW1 was increased by18.6%.Transgenic experiments showed that an appropriate expression level of TAW1 was necessary for panicle development.Haplotype analysis suggested that the favorable F771-type (Hap 13) of TAW1was introduced from aus accessions and had great potential value in high-yield breeding both in indica and japonica varieties.Our results provide a promising genetic resource for rice grain yield improvement. | Hua Yuan Zhengyan Xu Xueqin Tan Peng Gao Mengya Jin Wencheng Song Shiguang Wang Yunhai Kang Peixiong Liu Bin Tu Yuping Wang Peng Qin Shigui Li Bingtian Ma Weilan Chen | 2021 | The Crop Journal2021，9，5： | 2 |
| 16 | Kinetic Characteristics of Neutralizing Antibody Responses Vary among Patients with COVID-19显示文摘Objective The coronavirus disease 2019(COVID-19) pandemic continues to present a major challenge to public health. Vaccine development requires an understanding of the kinetics of neutralizing antibody(NAb) responses to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods In total, 605 serum samples from 125 COVID-19 patients(from January 1 to March 14, 2020)varying in age, sex, severity of symptoms, and presence of underlying diseases were collected, and antibody titers were measured using a micro-neutralization assay with wild-type SARS-CoV-2.Results NAbs were detectable approximately 10 days post-onset(dpo) of symptoms and peaked at approximately 20 dpo. The NAb levels were slightly higher in young males and severe cases, while no significant difference was observed for the other classifications. In follow-up cases, the NAb titer had increased or stabilized in 18 cases, whereas it had decreased in 26 cases, and in one case NAbs were undetectable at the end of our observation. Although a decreasing trend in NAb titer was observed in many cases, the NAb level was generally still protective.Conclusion We demonstrated that NAb levels vary among all categories of COVID-19 patients. Longterm studies are needed to determine the longevity and protective efficiency of NAbs induced by SARS-CoV-2. | LI Ling Hua TU Hong Wei LIANG Dan WEN Chun Yan LI An An LIN Wei Yin HU Ke Qi HONG Wen Shan LI Yue Ping SU Juan ZHAO San Tao LI Wei YUAN Run Yu ZHOU Ping Ping HU Feng Yu TANG Xiao Ping KE Chang Wen KE Bi Xia CAI Wei Ping | 2021 | Biomedical and Environmental Sciences2021，34，12： | 1 |
| 17 | Energy performance analysis on telecommunication base station 显示文摘 | Tu Rang Liu Xiao -hua Li zhen | 2011 | Energy and Buildings2011，43，23： | 1 |
| 18 | Levofloxacin‐containing Triple and Sequential Therapy or Standard Sequential Therapy as the First Line Treatment for Helicobacter pylori Eradication in China显示文摘 | Juan Qian Feng Ye Jun Zhang Yan‐Mei Yang Hui‐Ming Tu Qi Jiang Li Shang Xiao‐Lin Pan Rui‐Hua Shi Guo‐Xin Zhang | 2012 | Helicobacter2012，，6： | 1 |
| 19 | 一种用于小空间湿度控制的全固态湿泵显示文摘众多领域对湿度管理都有着广泛的需求,传统的除湿技术多以冷却冷凝或干燥剂除湿为主。但是,这些方法具有固有的一些缺点,比如说能耗大、体积大、结构复杂等。本文作者研究出一种全固态“湿泵”,它充分结合了商用热电模块和硅胶除湿剂的优点,在散热器表面覆盖硅胶涂层,使得材料与热源之间的接触热阻达到最小,同时也保证了干燥剂与空气之间留有足够的传热传质面积。 | Bangjun Li Lingji Hua Yaodong Tu Ruzhu Wang 李巧红 | 2019 | 家电科技2019，，3： | 1 |
| 20 | Role of the acidity and porosity of MWW-type zeolites in liquid-phase reaction显示文摘 | WANG J TU X HUA W | 2011 | Microporous and Mesoporous Materials2011，142，1： | 1 |
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