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| 1 | Construction and Identification of a Human Liver Specific MicroRNA Eukaryotic Expression Vector显示文摘MiR-122 is one of the non-coding RNAs which showed its effects on the lipo-metablism,virus infection and HCC forming through regulation of liver gene expression.Its eukaryotic expression vector was constructed by using pSuper which was widely applied in the siRNA expression.The precursor of human miR-122 gene was amplified by polymerase chain reaction(PCR)from the human genomic DNA.The positive clones were screened by PCR and restriction enzyme digestion.The new expression vector of miR-122 was named pHsa-m122.PHsa-m122 and its controls were transfected to HepG2 cells.The miR-122 expression activity was evaluated by GFP122i sensor reporter plasmid through fluorescence detection and Western blot.It was shown that the fluorescence intensity of GFP122si and pHsa-m122 co-transfection group was weaker than that of the controls,so the functional activity of expressed miR-122 was detected.When HepG2 cells were co-transfected with HBV1.3 and pHsa-m122 plasmids, the results showed miR-122 may down-regulate the gene expression of HBV.The human liver specific microRNA eukaryotic expression vector of miR-122 was constructed successfully,which may facilitate further study of its function in the development of liver virus infection diseases and HCC. | Shanshan Chen Ming Ni Bing Yu Tingting Lv Mengji Lu Feili Gong | 2007 | Cellular & Molecular Immunology2007，4，6： | 18 |
| 2 | The Coix Genome Provides Insights into Panicoideae Evolution and Papery Hull Domestication显示文摘Coix is a grass crop domesticated as early as the Neolithic era.It is still widely cultivated for both highly nutritional food and medicinal use.However,the genetic study and breeding of this crop are hindered by the lack of a sequenced genome.Here,we report de novo sequencing and assembly of the 1619-Mb genome of Coix,and annotation of 75.39%repeats and 39629 protein-coding genes.Comparative genomics analysis showed that Coix is more closely related to sorghum than maize,but intriguingly only Coix and maize had a recent genome duplication event,which was not detected in sorghum.We further constructed a genetic map and mapped several important traits,especially the strength of hull.Selection of papery hull(thin:easy dehulling)from the stony hull(thick:difficult dehulling)in wild progenitors was a key step in Coix domestication.The papery hull makes seed easier to process and germinate.Anatomic and global transcriptome analysis revealed that the papery hull is a result of inhibition of cell division and wall biogenesis.We also successfully demonstrated that seed hull pressure resistance is controlled by two major quantitative trait loci(QTLs),which are associated with hull thickness and color,respectively.The two QTLs were further fine mapped within intervals of 250 kb and 146 kb,respectively.These resources provide a platform for evolutionary studies and will facilitate molecular breeding of this important crop. | Chao Guo Yanan Wang Aiguo Yang Jun He Chaowen Xiao Shanhua Lv Fengming Han Yibing Yuan Yuan Yuan Xiaolong Dong Juan Guo YawenYang-Hailan Liu Ningzhi Zuo Yaxi Hu Kangxu Zhao Zhengbo Jiang Xing Wang Tingting Jiang Yaou Sherf Moju Cao Yuan Wang Zhaobo Long Tingzhao Rong Luqi Huang Shufeng Zhou | 2020 | Molecular Plant2020，13，2： | 11 |
| 3 | Single-cell RNA sequencing profiling of the effects of aging on alveolar stem cells显示文摘The aging of alveolar stem cells has been linked to many chronic lung diseases, including pulmonary fibrosis. However, the effects of aging on alveolar stem cells during homeostasis and post-injury alveolar repair have not been well characterized. Here we conducted a single-cell RNA sequencing(scRNA-seq) analysis of alveolar stem cells of 3-month-old and 12-month-old mice to characterize the aging effect on alveolar stem cells. Our results have shown that the transcriptomes of alveolar stem cells of 3-month-old and 12-month-old mice are not significantly different under the steady condition. However, after a bleomycin-induced lung injury, the alveolar stem cells of 12-month-old mice show enhanced inflammatory responses and decreased lipid metabolism. Our study suggests a close relationship among aging, lipid metabolism, inflammatory responses and chronic lung diseases. | Tingting Lv Kewu Jiang Jiawen Wang Nan Tang Huaping Dai Chen Wang | 2019 | Science China(Life Sciences)2019，62，8： | 6 |
| 4 | Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17显示文摘雌激素涉及支持肺癌症房间分割和转移。云母和 MICB 为 NKG2D 作为 ligands 工作,在生来的杀手(NK ) 上表示的重要 immunoreceptor 房间。然而,雌激素是否调整 MICA/B 表示并且影响肿瘤免疫者逃跑,仍然保持未知。在这研究,我们测量了非小的房间肺癌症(NSCLC ) 房间线与雌激素对待的云母, MICB 和 ADAM17in 的 mRNA 层次。LTEP-a2 和 A549 上的 MICA/B 的表面表示用流动 cytometry 被检测。我们证明 mRNA 和在肺腺癌房间线的 MICA/B 的能分泌的蛋白质层次是由 estradiol 的 upregulated。Estradiol 提高了 ADAM17 的表示,它与 MICA/B 的分泌物被联系。MICA/B downregulated 的这分泌物 NK92 房间的表面上的 NKG2D 受体并且损害了 NK 房间的细胞毒素的活动。Estradiol 提高了 ADAM17 的表示,它与 MICA/B 的分泌物被联系。而且,在 estradiol 和云母的表示的集中之间的重要关联在 NSCLC 病人的肿瘤纸巾被发现。因此,我们断定雌激素能通过 ADAM17 调整 MICA/B 的表示和分泌物,它帮助肺癌症房间逃离调停 NKG2D 的有免疫力的监视。 | Jing Ren Yunzhong Nie Mingming Lv Sunan Shen Ruijing Tang Yujun Xu Yayi Hou Shuli Zhao Tingting Wang | 2015 | Cellular & Molecular Immunology2015，12，6： | 6 |
| 5 | SIRT1 inhibits angiotensin Ⅱ-induced vascular smooth muscle cell hypertrophy显示文摘血管收缩素 II (Ang II ) 刺激脉管的光滑的肌肉房间(VSMC ) 肥大作为在象动脉粥样硬化那样的脉管的疾病的发展的一个批评事件。Sirtuin (SIRT ) 1,菸碱腺嘌 dinucleotide 依赖者 deacetylase,被表明了由支持脉管的松驰和减少的巨噬细胞泡沫房间形成,而是它在 VSMC 肥大的角色仍然是的内皮细胞层依赖者在动脉粥样硬化施加保护的效果未知。在这研究,我们试着在 Ang 导致 II 的 VSMC 肥大上调查 SIRT1 的效果。结果显著地显示出 SIRT1 的那调停 adenoviral 的在表示上禁止的 Ang 导致 II 的 VSMC 肥大,当时由 RNAi 的 SIRT1 击倒导致了一增加[3H ] VSMC 的白氨酸加入。因此,菸碱腺嘌 dinucleotide 磷酸盐 oxidase 1 (Nox1 ) Ang II 导致的表示被 SIRT1 在 VSMC 禁止。SIRT1 使活跃之物 resveratrol 减少了,而内长的 SIRT1 禁止者菸碱在 A7r5 VSMC 增加了 Nox1 表示。而且,抄写因素 GATA-6 被 SIRT1 涉及 Nox1 表示的下面规定。这些结果由压制 Ang 提供新卓见进 SIRT1 的 anti-atherogenic 性质导致 II 的 VSMC 肥大。 | Li Li Peng Gao Huina Zhang Houzao Chen Wei Zheng Xiang Lv Tingting Xu Yusheng Wei Depei Liu Chihchuan Liang | 2011 | Acta Biochimica et Biophysica Sinica2011，43，2： | 6 |
| 6 | Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from Sophora alopecuroides seeds显示文摘Sophora alopecuroides lectin (柳安) ,从 Sophora alopecuroides 的种子的新奇 lectin,被离子交换层析在 diethylaminoethyl (DEAE ) 上净化 - 并且 carboxymethyl (厘米)-Sepharose 列,在 Sephadex 75 10/300 GL 列上由胶化过滤列在后面。柳安被发现是 39916.3 Da 的单体,由 tricine 钠 dodecyl sulphate-polyacrylamide 胶化电气泳动和高效的液体层析决定了 HPLC ) 。柳安的 N 终端 10-amino 酸顺序, KPWALSFSFG,另外的荚 lectins 类似于那些。对在 11.9 g/ml 的兔子红血球的柳安展览 hemagglutinating 活动。它的 hemagglutinating 活动在 pH 范围是稳定的 711 并且在温度范围 3090 吗? | Tingting Li Xiaoli Yin Dongliang Liu Xiaojin Ma Hui Lv Surong Sun | 2012 | Acta Biochimica et Biophysica Sinica2012，44，7： | 6 |
| 7 | Potent anti-angiogenesis and anti-tumor activity of a nove human anti-VEGF antibody, MIL60显示文摘 | Jing Yang Qun Wang Chunxia Qiao Zhou Lin Xinying Li Yifei Huang Tingting Zhou Yan Li Beifen Shen Ming Lv Jiannan Feng | 2014 | Cellular & Molecular Immunology2014，11，3： | 4 |
| 8 | Selection and characterization of the novel anti-human PD-1 FV78 antibody from a targeted epitope mammalian cell-displayed antibody library显示文摘Currently,display-based methods are well established and widely used in antibody engineering for affinity maturation and structural stability improvement.We obtained a novel anti-human programmed death 1(PD-1)antibody using computer-aided design and a mammalian cell display technology platform.We used computer-aided modeling and distance geometry methods to predict and assign the key residues that contributed to the binding of human PD-L1 to PD-1.Then,we analyzed the sequence of nivolumab(an anti-human PD-1 antibody,referred to as MIL75 in the article)to determine the template for antibody design and library construction.We identified a series of potential substitutions on the obtained template and constructed a virtual epitope-targeted antibody library based on the physicochemical properties and each possible location of the assigned key residues.The virtual antibody libraries were displayed on the surface of mammalian cells as the antigen-binding fragments of full-length immunoglobulin G.Then,we used flow cytometry and sequencing approaches to sort and screen the candidates.Finally,we obtained a novel anti-human PD-1 antibody named FV78.FV78 competitively recognized the PD-1 epitopes that interacted with MIL75 and possessed an affinity comparable to MIL75.Our results implied that FV78 possessed equivalent bioactivity in vitro and in vivo compared with MIL75,which highlighted the probability and prospect of FV78 becoming a new potential antibody therapy. | Longlong Luo Shi Wang Xiaoling Lang Tingting Zhou Jing Geng Xinying Li Chunxia Qiao Jiannan Feng Beifen Shen Ming Lv Yan Li | 2018 | Cellular & Molecular Immunology2018，15，2： | 4 |
| 9 | In vivo dissolution of poorly water-soluble drugs:Proof of concept based on fluorescence bioimaging显示文摘In vitro-in vivo correlation(IVIVC)of solid dosage forms should be established basically between in vitro and in vivo dissolution of active pharmaceutical ingredients.Nevertheless,in vivo dissolution profles have never been accurately portrayed.The current practice of IVIVC has to resort to in vivo absorption fractions(Fa).In this proof-of-concept study,in vivo dissolution of a model poorly watersoluble drug fenofbrate(FNB)was investigated by fuorescence bioimaging.FNB crystals were frst labeled by near-infrared fuorophores with aggregation-caused quenching properties.The dyes illuminated FNB crystals but quenched immediately and absolutely once been released into aqueous media,enabling accurate monitoring of residual drug crystals.The linearity established between fuorescence and crystal concentration justifed reliable quantifcation of FNB crystals.In vitro dissolution was frst measured following pharmacopoeia monograph protocols with well-documented IVIVC.The synchronicity between fuorescence and in vitro dissolution of FNB supported using fuorescence as a measure for determination of dissolution.In vitro dissolution correlated well with in vivo dissolution,acquired by either live or ex vivo imaging.The newly established IVIVC was further validated by correlating both in vitro and in vivo dissolution with Faobtained from pharmacokinetic data. | Yinqian Yang Yongjiu Lv Chengying Shen Tingting Shi Haisheng He Jianping Qi Xiaochun Dong Weili Zhao Yi Lu Wei Wu | 2021 | Acta Pharmaceutica Sinica B2021，11，4： | 3 |
| 10 | Herbal formula of Bushen Jianpi(补肾健脾方)combined with sorafenib inhibits hepatocellular carcinoma growth by promoting cell apoptosis and blocking the cell cycle显示文摘OBJECTIVE:To investigate the efficacy of an herbal formula of Bushen Jianpi(补肾健脾方,BSJP)combined with sorafenib on hepatocellular carcinoma(HCC)in vitro and in vivo,and to study the underlying mechanisms of action.METHODS:BSJP,a mixture of 12 raw herbs,was extracted in 70%alcohol/30%water and freeze-dried into a powder.The in vitro effects of BSJP alone,sorafenib alone,and their combination on cell survival,apoptosis,and cell cycle distribution were evaluated in HCC cell lines HCCLM3,HepG2,and SMMC-7721.The expression of B-cell lymphoma-2(Bcl-2),caspase-3,and caspase-9 in HCCLM3 cells was measured using Western blots after drug administration.The in vivo effects of BSJP and sorafenib were evaluated in a tumor surgical resection model using 4-week old male athymic BALB/c nude mice injected with HCCLM3 cells.Immunohistochemical analysis of tumor tissues was performed to evaluate the effects of BSJP alone,sorafenib alone,and their combination on the expression of caspase-3,caspase-9,and Bcl-2.RESULTS:BSJP decreased the survival rate of HCC cell lines,and the combination of BSJP and sorafenib further decreased the survival rate.BSJP significantly promoted cell apoptosis and blocked cell-cycle progression in HCCLM3,HepG2,and SMMC-7721 cells in a dose-dependent manner.Furthermore,the administration of BSJP and sorafenib inhibited the growth of HCCLM3 cell xenografts in nude mice,with no reduction in body weight.In vivo and in vitro experiments showed that BSJP combined with sorafenib could significantly decrease the expression of Bcl-2.CONCLUSION:Our findings suggest that the herbal formula of BSJP is a potential HCC antitumor agent. | ZHOU Zhangjie LIU Xinhua WU Tingting QUE Zujue WU Zhonghua WU Weizhong FU Shujuan ZHANG Shiqiang YANG Yun JIANG Haiyan XIA Xiaoting LV Junqiang DU Boqian LI Yun LU Tao ZHANG Zhihui ZHONG Yi | 2021 | Journal of Traditional Chinese Medicine2021，41，2： | 3 |
| 11 | Modes of genetic adaptations underlying functional innovations in the rumen显示文摘The rumen is the hallmark organ of ruminants and hosts a diverse ecosystem of microorganisms that facilitates efficient digestion of plant fibers.We analyzed 897 transcriptomes from three Cetartiodactyla lineages:ruminants,camels and cetaceans,as well as data from ruminant comparative genomics and functional assays to explore the genetic basis of rumen functional innovations.We identified genes with relatively high expression in the rumen,of which many appeared to be recruited from other tissues.These genes show functional enrichment in ketone body metabolism,regulation of microbial community,and epithelium absorption,which are the most prominent biological processes involved in rumen innovations.Several modes of genetic change underlying rumen functional innovations were uncovered,including coding mutations,genes newly evolved,and changes of regulatory elements.We validated that the key ketogenesis rate-limiting gene(HMGCS2)with five ruminant-specific mutations was under positive selection and exhibits higher synthesis activity than those of other mammals.Two newly evolved genes(LYZ1 and DEFB1)are resistant to Gram-positive bacteria and thereby may regulate microbial community equilibrium.Furthermore,we confirmed that the changes of regulatory elements accounted for the majority of rumen gene recruitment.These results greatly improve our understanding of rumen evolution and organ evo-devo in general. | Xiangyu Pan Yudong Cai Zongjun Li Xianqing Chen Rasmus Heller Nini Wang Yu Wang Chen Zhao Yong Wang Han Xu Songhai Li Ming Li Cunyuan Li Shengwei Hu Hui Li Kun Wang Lei Chen Bin Wei Zhuqing Zheng Weiwei Fu Yue Yang Tingting Zhang Zhuoting Hou Yueyang Yan Xiaoyang Lv Wei Sun Xinyu Li Shisheng Huang Lixiang Liu Shengyong Mao Wenqing Liu Jinlian Hua Zhipeng Li Guojie Zhang Yulin Chen Xihong Wang Qiang Qiu Brian PDalrymple Wen Wang Yu Jiang | 2021 | Science China(Life Sciences)2021，64，1： | 3 |
| 12 | Study of two-nutrient and two-micro-organism chemostat model with pulsed input in a polluted environment显示文摘 | Jianwen Jia Tingting Lv | 2015 | International Journal of Biomathematics2015，8，4： | 2 |
| 13 | Research on energy storage capacity configuration for PV power plants using uncertainty analysis and its applications显示文摘Compensating for photovoltaic(PV)power forecast errors is an important function of energy storage systems.As PV power outputs have strong random fluctuations and uncertainty,it is difficult to satisfy the grid-connection requirements using fixed energy storage capacity configuration methods.In this paper,a method of configuring energy storage capacity is proposed based on the uncertainty of PV power generation.A k-means clustering algorithm is used to classify weather types based on differences in solar irradiance.The power forecast errors in different weather types are analyzed,and an energy storage system is used to compensate for the errors.The kernel density estimation is used to fit the distributions of the daily maximum power and maximum capacity requirements of the energy storage system;the power and capacity of the energy storage unit are calculated at different confidence levels.The optimized energy storage configuration of a PV plant is presented according to the calculated degrees of power and capacity satisfaction.The proposed method was validated using actual operating data from a PV power station.The results indicated that the required energy storage can be significantly reduced while compensating for power forecast errors. | Honglu Zhu Ruyin Hou Tingting Jiang Qingquan Lv | 2021 | Global Energy Interconnection2021，4，6： | 2 |
| 14 | Spatial transcriptome analysis of long non-coding RNAs reveals tissue specificity and functional roles in cancer显示文摘Long non-coding RNAs(lncRNAs)play a significant role in maintaining tissue morphology and functions,and their precise regulatory effectiveness is closely related to expression patterns.However,the spatial expression patterns of lncRNAs in humans are poorly characterized.Here,we constructed five comprehensive transcriptomic atlases of human lncRNAs covering thousands of major tissue samples in normal and disease states.The lncRNA transcriptomes exhibited high consistency within the same tissues across resources,and even higher complexity in specialized tissues.Tissue-elevated(TE)lncRNAs were identified in each resource and robust TE lncRNAs were refined by integrative analysis.We detected 1 to 4684 robust TE lncRNAs across tissues;the highest number was in testis tissue,followed by brain tissue.Functional analyses of TE lncRNAs indicated important roles in corresponding tissue-related pathways.Moreover,we found that the expression features of robust TE lncRNAs made them be effective biomarkers to distinguish tissues;TE lncRNAs also tended to be associated with cancer,and exhibited differential expression or were correlated with patient survival.In summary,spatial classification of lncRNAs is the starting point for elucidating the function of lncRNAs in both maintenance of tissue morphology and progress of tissue-constricted diseases. | Kang XU Xiyun JIN Ya LUO Haozhe ZOU Dezhong LV Liping WANG Limei FU Yangyang CAI Tingting SHAO Yongsheng LI Juan XU | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，1： | 1 |
| 15 | Guidelines on the Diagnosis and Management of Primary Biliary Cholangitis (2021)显示文摘In 2015, the Chinese Society of Hepatology and the Chinese Society of Gastroenterology published a consensus on pri-mary biliary cholangitis (PBC). In the past years, numerous clinical studies have been published in the field of PBC. To guide the clinical diagnosis and management of PBC patients, the Chinese Society of Hepatology invited a panel of experts to assess the new clinical evidence and formulate the current guidelines. | Hong You Weijia Duan Shuxiang Li Tingting Lv Sha Chen Lungen Lu Xiong Ma Ying Han Yuemin Nan Xiaoyuan Xu Zhongping Duan Lai Wei Jidong Jia Hui Zhuang Chinese Society of Hepatology,Chinese Medical Association | 2023 | Journal of Clinical and Translational Hepatology2023，11，3： | 1 |
| 16 | Protein convertase subtilisin/Kexin type 9 inhibits hepatocellular carcinoma growth by interacting with GSTP1 and suppressing the JNK signaling pathway显示文摘Objective:Protein convertase subtilisin/Kexin type 9(PCSK9)has been found to be closely associated with the occurrence and development of numerous tumors.However,the precise role of PCSK9 and its relationship to the development of hepatocellular carcinoma(HCC)remain largely unknown.This study aimed to clarify these issues.Methods:The expression levels of PCSK9 in HCC tissues and HCC cell lines were determined by the quantitative reverse transcription polymerase chain reaction,Western blot,and immunohistochemical analyses,and the effects of PCSK9 expression on HCC cell biological traits were investigated by overexpressing and downregulating PCSK9 expression in vivo and in vitro.Additionally,the mechanism by which PCSK9 mediated dissociation of glutathione S-transferase Pi 1(GSTP1)dimers and phosphorylation of the Jun N-terminal kinase(JNK)pathway components were investigated.Results:PCSK9 expression levels were significantly lower in HCC tissues than in adjacent non-tumor samples.In vivo and in vitro experiments suggested that PCSK9 inhibited HCC cell proliferation and metastasis.Further analysis showed that PCSK9 interacted with GSTP1 and promoted GSTP1 dimer dissociation and JNK signaling pathway inactivation in HCC cells.Moreover,the relationships between PCSK9 protein expressions and clinical outcomes were investigated.The PCSK9-lo group displayed a significantly shorter overall survival(OS;median OS:64.2 months vs.83.2 months;log-rank statistic:4.237;P=0.04)and recurrencefree survival(RFS;median RFS:26.5 months vs.46.6 months;log-rank statistic:10.498;P=0.001)time than the PCSK9-hi group.Conclusions:PCSK9 inhibited HCC cell proliferation,cell cycle progression,and apoptosis by interacting with GSTP1 and suppressing JNK signaling,suggesting that PCSK9 might act as a tumor suppressor and be a therapeutic target in HCC patients. | Mingyan He Jing Hu Tingting Fang Wenqing Tang Bei Lv Biwei Yang Jinglin Xia | 2022 | Cancer Biology & Medicine2022，19，1： | 1 |
| 17 | Synthesis, structure and biological activity of cobalt ( Ⅱ ) and copper (Ⅱ) complexes of valine-derived schiff bases显示文摘 | LV Jian LIU Tingting CAI Sulan | 2006 | J Inorg Bioehem2006，100，11： | 1 |
| 18 | Stability of Sn-Pb mixed organic–inorganic halide perovskite solar cells:Progress,challenges,and perspectives显示文摘The exploration of low bandgap perovskite material to approach Shockley-Queisser limit of photovoltaic device is of great significance,but it is still challenging.During the past few years,tin–lead(Sn-Pb)mixed perovskites with low bandgaps have been rapidly developed,and their single junction solar cells have reached power conversion efficiency(PCE)over 21%,which also makes them ideal candidate as low bandgap sub-cell for tandem device.Nevertheless,due to the incorporation of unstable Sn^(2+),the stability issue becomes the vital problem for the further development of Sn-Pb mixed perovskite solar cells(PSCs).In this review,we are dedicated to give a full view in current understanding on the stability issue of SnPb mixed perovskites and their PSCs.We begin with the demonstration on the origin of instability of Sn-Pb mixed perovskites,including oxidation of Sn^(2+),defects,and interfacial layer induced instability.Sequentially,the up-to-date developments on the stability improvement of Sn-Pb mixed perovskites and their PSCs is systematically reviewed,including composition engineering,additive engineering,and interfacial engineering.At last,the current challenges and future perspectives on the stability study of Sn-Pb mixed PSCs are discussed,which we hope could promote the further application of Sn-Pb mixed perovskites towards commercialization. | Shaoshen Lv Weiyin Gao Yanghua Liu He Dong Nan Sun Tingting Niu Yingdong Xia Zhongbin Wu Lin Song Chenxin Ran Li Fu Yonghua Chen | 2022 | Journal of Energy Chemistry2022，31，2： | 1 |
| 19 | Development,validation and comparison of multivariable risk scores for prediction of total stroke and stroke types in Chinese adults:a prospective study of 0.5 million adults显示文摘Background and purpose Low-income and middle-income countries have the greatest stroke burden,yet remain understudied.This study compared the utility of Framingham versus novel risk scores for prediction of total stroke and stroke types in Chinese adults.Methods China Kadoorie Biobank(CKB)is a prospective study of 512726 adults,aged 30-79 years,recruited from 10 areas in China in 2004-2008.By 1 January 2018,43234 incident first stroke cases(36310 ischaemic stroke(IS);8865 haemorrhagic stroke(HS))were recorded in 503842 participants with no history of stroke at baseline.We compared the predictive utility of the Framingham Stroke Risk Profile(FSRP)with novel CKB stroke risk scores and included recalibration,refitting,stratifying by study area and addition of other risk factors.Discrimination was assessed using area under the receiver operating characteristic curve(AUC)and calibration was assessed using Greenwood-Nam-D’Agostinoχ^(2) statistics.Results Incidence of total stroke varied fivefold by area in China.The FSRP had good discrimination for total stroke(AUC(95%CI);men:0.78(0.77 to 0.79),women:0.77(95%CI 0.76 to 0.78)),but poor calibration(χ^(2);men:1,825,women:3,053),substantially underestimating absolute risks.Recalibration reducedχ^(2) by>80%,but did not improve discrimination.Refitting the FSRP did not materially improve discrimination,but further improved calibration.Stratification by area improved discrimination(AUC;men:0.82(0.82 to 0.83);women:0.82(0.82 to 0.83)),but not calibration.Adding other risk factors yielded modest,but statistically significant,improvements in the AUCs.The findings for IS and HS were similar to those for total stroke.Conclusions The FSRP reliably differentiated Chinese adults with incident stroke,but substantially underestimated the absolute risks of stroke.Novel local risk prediction equations that took account of differences in stroke incidence within China enhanced risk prediction of total stroke and major stroke pathological types. | Matthew Chun Robert Clarke Tingting Zhu David Clifton Derrick A Bennett Yiping Chen Yu Guo Pei Pei Jun Lv Canqing Yu Ling Yang Liming Li Zhengming Chen Benjamin J Cairns 无 | 2022 | Stroke & Vascular Neurology2022，7，4： | 1 |
| 20 | Traffic Allocation Scheme with Cooperation of Multiple RANs in Universal Wireless Environments显示文摘This paper introduces an adaptive traffic allocation scheme with cooperation of multiple Radio Access Networks (RANs) in universal wireless environments.The different cooperation scenarios are studied,and based on the scenario of cooperation in both network layer and terminal layer,an open queuing system model,which is aiming to depict the characteristics of packet loss rate of wireless communication networks,is proposed to optimize the traffic allocation results.The analysis and numerical simulations indicate that the proposed scheme achieves inter-networking load balance tominimize the whole transmission delay and expands the communication ability of single-mode terminals to support high data rate traffics. | Lv Zhaobiao Sun Lei Wang Jianquan Ran Hui Zhao Tingting | 2012 | China Communications2012，9，4： | 1 |
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