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| 1 | AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells显示文摘The current coronavirus disease 2019(COVID-19)pandemic presents a global public health challenge.The viral pathogen responsible,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),binds to the host receptor ACE2 through its spike(S)glycoprotein,which mediates membrane fusion and viral entry.Although the role of ACE2 as a receptor for SARS-CoV-2 is clear,studies have shown that ACE2 expression is extremely low in various human tissues,especially in the respiratory tract.Thus,other host receptors and/or co-receptors that promote the entry of SARS-CoV-2 into cells of the respiratory system may exist.In this study,we found that the tyrosine-protein kinase receptor UFO(AXL)specifically interacts with the N-terminal domain of SARS-CoV-2 S.Using both a SARS-CoV-2 virus pseudotype and authentic SARS-CoV-2,we found that overexpression of AXL in HEK293T cells promotes SARS-CoV-2 entry as efficiently as overexpression of ACE2,while knocking out AXL significantly reduces SARS-CoV-2 infection in HI 299 pulmonary cells and in human primary lung epithelial cells.Soluble human recombinant AXL blocks SARS-CoV-2 infection in cells expressing high levels of AXL.The AXL expression level is well correlated with SARS-CoV-2 S level in bronchoalveolar lavage fluid cells from COVID-19 patients.Taken together,our findings suggest that AXL is a novel candidate receptor for SARS-CoV-2 which may play an important role in promoting viral infection of the human respiratory system and indicate that it is a potential target for future clinical intervention strategies. | Shuai Wang Zongyang Qiu Yingnan Hou Xiya Deng Wei Xu Tingting Zheng Peihan Wu Shaofang Xie Weixiang Bian Chong Zhang Zewei Sun Kunpeng Liu Chao Shan Aifu Lin Shibo Jiang Youhua Xie Qiang Zhou Lu Lu Jing Huang Xu Li | 2021 | Cell Research2021，31，2： | 39 |
| 2 | Kinsenoside attenuates osteoarthritis by repolarizing macrophages through inactivating NF-κB/MAPK signaling and protecting chondrocytes显示文摘The objective was to investigate the effect of kinsenoside(Kin) treatments on macrophage polarity and evaluate the resulting protection of chondrocytes to attenuate osteoarthritis(OA) progression.RAW264.7 macrophages were polarized to M1/M2 subtypes then administered with different concentrations of Kin. The polarization transitions were evaluated with quantitative real-time polymerase chain reaction(q RT-PCR), confocal observation and flow cytometry analysis. The mechanism of Kin repolarizing M1 macrophages was evaluated by Western blot. Further, macrophage conditioned medium(CM) and IL-1β were administered to chondrocytes. Micro-CT scanning and histological observations were conducted in vivo on anterior cruciate ligament transection(ACLT) mice with or without Kin treatment. We found that Kin repolarized M1 macrophages to the M2 phenotype. Mechanistically, Kin inhibited the phosphorylation of IκBα, which further reduced the downstream phosphorylation of P65 in nuclear factor-κB(NF-κB) signaling. Moreover, Kin inhibited mitogen-activated protein kinases(MAPK) signaling molecules p-JNK, p-ERK and p-P38. Additionally, Kin attenuated macrophage CM and IL-1β-induced chondrocyte damage. In vivo, Kin reduced the infiltration of M1 macrophages,promoted M2 macrophages in the synovium, inhibited subchondral bone destruction and reduced articular cartilage damage induced by ACLT. All the results indicated that Kin is an effective therapeutic candidate for OA treatment. | Feng Zhou Jingtian Mei Xiuguo Han Hanjun Li Shengbing Yang Minqi Wang Linyang Chu Han Qiao Tingting Tang | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 35 |
| 3 | SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2显示文摘 | Huize Pan Di Guan Xiaomeng Liu Jingyi Li Lixia Wang Jun Wu Junzhi Zhou Weizhou Zhang Ruotong Ren Weiqi Zhang Ying Li Jiping Yang Ying Hao Tingting Yuan Guohong Yuan Hu Wang Zhenyu Ju Zhiyong Mao Jian Li Jing Qu FuchouTang Guang-Hui Liu | 2016 | Cell Research2016，26，2： | 32 |
| 4 | Laser cleaning of steel structure surface for paint removal and repaint adhesion显示文摘Paint removal from steel structure is executed for shipyards of marine and offshore engineering.Due to environmental unfriendliness and unhealthy drawbacks of sand blasting technique, laser ablation technique is proposed as a substituting method.By absorbing high energy of the 1064 nm pulsed laser, the paint is vaporized quickly.The ablated debris is then collected by using a suction pump.Initial metal surface of the steel is exposed when laser beam irradiates perpendicularly and scans over it.The cleaned surface fulfills the requirements of surface preparation standards ISO 8501 of SA2.The adhesion is further characterized with pull-off test after carrying out painting with Jotamastic 87 aluminum paint.The repainting can be embedded onto the laser cleaned surface to bond much more tightly.The excellent adhesion strength of 20 MPa between repainted coating and the substrate is achieved, which is higher than what is required by shipyards applications. | Xiaoguang Li Tingting Huang Ang Wei Chong Rui Zhou Yoo Sang Choo Minghui Hong | 2017 | 光电工程2017，44，3： | 24 |
| 5 | Circulating apoptotic bodies maintain mesenchymal stem cell homeostasis and ameliorate osteopenia via transferring multiple cellular factors显示文摘 | Dawei Liu Xiaoxing Kou Chider Chen Shiyu Liu Yao Liu Wenjing Yu Tingting Yu Ruili Yang Runci Wang Yanheng Zhou Songtao Shi | 2018 | Cell Research2018，28，9： | 17 |
| 6 | Chemical and optical properties of aerosols and their interrelationship in winter in the megacity Shanghai of China显示文摘A field campaign on air quality was carried out in Shanghai in winter of 2012. The concentrations of NO, NO2, NOx, SO2, CO, and PM2.5increased during haze formation. The average masses of SO42-, NO3-and NH4+were 10.3, 11.7 and 6.7 μg/m3 during the haze episodes, which exceeded the average(9.2, 7.9, and 3.4 μg/m3) of these components in the non-haze days. The mean values for the aerosol scattering coefficient(b sp), aerosol absorption coefficient(b ap) and single scattering albedo(SSA) were 288.7, 27.7 and0.91 Mm-1, respectively. A bi-peak distribution was observed for the mass concentrations of CO, NO, NO2, and NOx. More sulfate was produced during daytime than that in the evening due to photochemical reactions. The mass concentration of NH4+achieved a small peak at noontime. NO3-showed lower concentrations in the afternoon and higher concentrations in the early morning. There were obvious bi-peak diurnal patterns for b sp and b ap as well as SSA. b sp and b ap showed a positive correlation with PM2.5mass concentration.(NH4)2SO4, NH4NO3, organic mass, elemental carbon and coarse mass accounted for 21.7%, 19.3%, 31.0%, 9.3% and 12.3% of the total extinction coefficient during non-haze days, and 25.6%, 24.3%, 30.1%, 8.1% and 8.2% during hazy days. Organic matter was the largest contributor to light extinction. The contribution proportions of ammonium sulfate and ammonium nitrate to light extinction were significantly higher during the hazy time than during the non-haze days. | Tingting Han Liping Qiao Min Zhou Yu Qu Jianfei Du Xingang Liu Shengrong Lou Changhong Chen Hongli Wang Fang Zhang Qing Yu Qiong Wu | 2015 | Journal of Environmental Sciences2015，27，1： | 14 |
| 7 | Platelet-rich fibrin-induced bone marrow mesenchymal stem cell differentiation into osteoblast-like cells and neural cells显示文摘Bone marrow mesenchymal stem cells were allowed to develop for 14 days in a platelet-rich fibrin environment.Results demonstrated that platelet-rich fibrin significantly promoted bone marrow mesenchymal stem cell proliferation.In addition,there was a dose-dependent increase in Runt-related transcription factor-2 and bone morphogenetic protein-2 mRNA expression,as well as neuron-specific enolase and glial acidic protein.Results showed that platelet-rich fibrin promoted bone marrow mesenchymal stem cell proliferation and differentiation of osteoblast-like cells and neural cells in a dose-dependent manner. | Qi Li Yajun Geng Lei Lu Tingting Yang Mingrui Zhang Yanmin Zhou | 2011 | Neural Regeneration Research2011，6，31： | 12 |
| 8 | Landscape of DILI-related adverse drug reaction in China Mainland显示文摘Drug-induced liver injury(DILI)is a type of bizarre adverse drug reaction(ADR)damaging liver(L-ADR)which may lead to substantial hospitalizations and mortality.Due to the general low incidence,detection of L-ADR remains an unsolved public health challenge.Therefore,we used the data of 6.673 million of ADR reports from January 1st,2012 to December 31st,2016 in China National ADR Monitoring System to establish a new database of L-ADR reports for future investigation.Results showed that totally 114,357 ADR reports were retrieved by keywords searching of liver-related injuries from the original heterogeneous system.By cleaning and standardizing the data fields by the dictionary of synonyms and English translation,we resulted 94,593 ADR records reported to liver injury and then created a new database ready for computer mining.The reporting status of L-ADR showed a persistent 1.62-fold change over the past five years.The national population-adjusted reporting numbers of L-ADR manifested an upward trend with age increasing and more evident in men.The annual reporting rate of L-ADR in age group over 80 years old strikingly exceeded the annual DILI incidence rate in general population,despite known underreporting situation in spontaneous ADR reporting system.The percentage of herbal and traditional medicines(H/TM)L-ADR reports in the whole number was 4.5%,while 80.60%of the H/TM reports were new findings.There was great geographical disparity of reported agents,i.e.more cardiovascular and antineoplastic agents were reported in higher socio-demographic index(SDI)regions and more antimicrobials,especially antitubercular agents,were reported in lower SDI regions.In conclusion,this study presented a large-scale,unbiased,unified,and computer-minable L-ADR database for further investigation.Age-,sex-and SDI-related risks of L-ADR incidence warrant to emphasize the precise pharmacovigilance policies within China or other regions in the world. | Jiabo Wang Haibo Song Feilin Ge Peng Xiong Jing Jing Tingting He Yuming Guo Zhuo Shi Chao Zhou Zixin Han Yanzhong Han Ming Niu Zhaofang Bai Guangbin Luo Chuanyong Shen Xiaohe Xiao | 2022 | Acta Pharmaceutica Sinica B2022，12，12： | 12 |
| 9 | The Coix Genome Provides Insights into Panicoideae Evolution and Papery Hull Domestication显示文摘Coix is a grass crop domesticated as early as the Neolithic era.It is still widely cultivated for both highly nutritional food and medicinal use.However,the genetic study and breeding of this crop are hindered by the lack of a sequenced genome.Here,we report de novo sequencing and assembly of the 1619-Mb genome of Coix,and annotation of 75.39%repeats and 39629 protein-coding genes.Comparative genomics analysis showed that Coix is more closely related to sorghum than maize,but intriguingly only Coix and maize had a recent genome duplication event,which was not detected in sorghum.We further constructed a genetic map and mapped several important traits,especially the strength of hull.Selection of papery hull(thin:easy dehulling)from the stony hull(thick:difficult dehulling)in wild progenitors was a key step in Coix domestication.The papery hull makes seed easier to process and germinate.Anatomic and global transcriptome analysis revealed that the papery hull is a result of inhibition of cell division and wall biogenesis.We also successfully demonstrated that seed hull pressure resistance is controlled by two major quantitative trait loci(QTLs),which are associated with hull thickness and color,respectively.The two QTLs were further fine mapped within intervals of 250 kb and 146 kb,respectively.These resources provide a platform for evolutionary studies and will facilitate molecular breeding of this important crop. | Chao Guo Yanan Wang Aiguo Yang Jun He Chaowen Xiao Shanhua Lv Fengming Han Yibing Yuan Yuan Yuan Xiaolong Dong Juan Guo YawenYang-Hailan Liu Ningzhi Zuo Yaxi Hu Kangxu Zhao Zhengbo Jiang Xing Wang Tingting Jiang Yaou Sherf Moju Cao Yuan Wang Zhaobo Long Tingzhao Rong Luqi Huang Shufeng Zhou | 2020 | Molecular Plant2020，13，2： | 11 |
| 10 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update of the management of high-risk population显示文摘Aim Cerebrovascular disease is the leading cause of death and disability in China,causing a huge burden among patients and their families.Hence,stroke prevention is critical,especially in the high-risk population.Here,we present the evidence-based guideline suitable for the Chinese population.Methods Literature search of PubMed and Cochrane library(from January 1964 to June 2019)was done.After thorough discussion among the writing group members,recommendations were listed and summarised.This guideline was reviewed and discussed by the fellow writing committees of the Chinese Stroke Association’s Stroke.Results This evidence-based guideline was written in three parts:controlling the risk factors of stroke,utilisation of antiplatelet agents and assessing the risks of first-ever stroke.All recommendations were listed along with the recommending classes and levels of evidence.Conclusions This guideline provides recommendations for primary prevention of cerebrovascular disease among high-risk population in China.Controlling related risk factors,appropriately using antiplatelet agents,assessing the risk of developing first-ever stroke should help reduce the rate of cerebrovascular disease in China. | Yilong Wang Shangrong Han Haiqiang Qin Huaguang Zheng Bin Jiang Yong Cao Yuan Gao Ling Guan Qian Jia Yong Jiang Yuming Jiao Shuya Li Yapeng Li Zixiao Li Wei Liu Xiaojuan Ru Dongling Sun Haixin Sun Penglian Wang Tingting Wang Lixia Zong Lei Guo Xuewei Xie Yuming Xu Yuyuan Xu Xiaomeng Yang Yingying Yang Mengyuan Zhou Wenzhi Wang Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 11 |
| 11 | Solution structure of the RNA recognition domain of METTL3-METTL14 N^6-methyladenosine methyltransferase显示文摘N^6-methyladenosine(m6A),a ubiquitous RNA modification,is installed by METTL3-METTL14 complex.The structure of the heterodimeric complex between the methyltransferase domains(MTDs)of METTL3 and METTL14 has been previously determined.However,the MTDs alone possess no enzymatic activity.Here we present the solution structure for the zinc finger domain(ZFD)of METTL3,the inclusion of which fulfills the methyltransferase activity of METTL3-METTL14.We show that the ZFD specifically binds to an RNA containing 5'-GGACU-3'consensus sequence,but does not to one without.The ZFD thus serves as the target recognition domain,a structural feature previously shown for DNA methyltransferases,and cooperates with the MTDs of METTL3-METTL14 for catalysis.However,the interaction between the ZFD and the specific RNA is extremely weak,with the binding affinity at several hundred micromolar under physiological conditions.The ZFD contains two CCCH-type zinc fingers connected by an anti-parallel P-sheet.Mutational analysis and NMR titrations have mapped the functional interface to a contiguous surface.As a division of labor,the RNA-binding interface comprises basic residues from zinc finger 1 and hydrophobic residues fromβ-sheet and zinc finger 2.Further we show that the linker between the ZFD and MTD of METTL3 is flexible but partially folded,which may permit the cooperation between the two domains during catalysis.Together,the structural characterization of METTL3 ZFD paves the way to elucidate the atomic details of the entire process of RNA m6A modification. | Jinbo Huang Xu Dong Zhou Gong Ling-Yun Qin Shuai Yang Yue-Ling Zhu Xiang Wang Delin Zhang Tingting Zou Ping Yin Chun Tang | 2019 | Protein & Cell2019，10，4： | 11 |
| 12 | miR-21 promotes the differentiation of hair folliclederived neural crest stem cells into Schwann cells显示文摘Hair follicle-derived neural crest stem cells can be induced to differentiate into Schwann cells in vivo and in vitro. However, the underlying regulatory mechanism during cell differentiation remains poorly understood. This study isolated neural crest stem cells from human hair follicles and induced them to differentiate into Schwann cells. Quantitative RT-PCR showed that microRNA(miR)-21 expression was gradually increased during the differentiation of neural crest stem cells into Schwann cells. After transfection with the miR-21 agonist(agomir-21), the differentiation capacity of neural crest stem cells was enhanced. By contrast, after transfection with the miR-21 antagonist(antagomir-21), the differentiation capacity was attenuated. Further study results showed that SOX-2 was an effective target of miR-21. Without compromising SOX2 mRNA expression, miR-21 can down-regulate SOX protein expression by binding to the 3′-UTR of miR-21 mRNA. Knocking out the SOX2 gene from the neural crest stem cells significantly reversed the antagomir-21 inhibition of neural crest stem cells differentiating into Schwann cells. The results suggest that miR-21 expression was increased during the differentiation of neural crest stem cells into Schwann cells and miR-21 promoted the differentiation through down-regulating SOX protein expression by binding to the 3′-UTR of SOX2 mRNA. | Yuxin Ni Kaizhi Zhang Xuejuan Liu Tingting Yang Baixiang Wang Li Fu Lan A Yanmin Zhou | 2014 | Neural Regeneration Research2014，9，8： | 9 |
| 13 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 14 | Cardiac BIN1(cBIN1) is a regulator of cardiac contractile function and an emerging biomarker of heart muscle health显示文摘In recent decades,a cardiomyocyte membrane scaffolding protein bridging integrator 1(BIN1) has emerged as a critical multifunctional regulator of transverse-tubule(t-tubule) function and calcium signaling in cardiomyocytes.Encoded by a single gene with 20 exons that are alternatively spliced,more than ten BIN1 protein isoforms are expressed with tissue and disease specificity.The recently discovered cardiac alternatively spliced isoform BIN1(cBIN1 or BIN1 +13 + 17)plays a crucial role in organizing membrane microfolds within cardiac t-tubules.These cBIN1-induced microfolds form functional dyad microdomains by trafficking L-type calcium channels(LTCC) to t-tubule membrane and recruiting ryanodine receptors(RyR) to junctional sarcoplasmic reticulum membrane.When cBIN1 is transcriptionally reduced as occurs in heart failure,cBIN1-microfolds are disrupted and fail to form LTCC and RyR couplons.As a result,impaired dyad formation limits excitation-contraction coupling thus cardiac contractility,and accumulation of orphaned leaky RyRs outside of dyads increases ventricular arrhythmias.Reduced myocardial BIN1 in heart failure is also detectable at the blood level,and plasma BIN1 level predicts heart failure progression and future arrhythmias in cardiomyopathy patients.Here we will review the recent progress in BIN1-related cardiomyocyte biology studies and discuss the diagnostic and predictive values of cBIN1 in future clinical use. | Kang Zhou Tingting Hong | 2017 | Science China(Life Sciences)2017，60，3： | 8 |
| 15 | The geochemical characteristics,geochronology and tectonic significance of the Carboniferous volcanic rocks of the Santanghu area in northeastern Xinjiang,China显示文摘The Santanghu area is located on the northeastern margin of the Junggar Basin,northern Xinjiang,Northwest China.The Carboniferous volcanic rocks in this area are widely distributed in Kaokesaiergaishan,Santanghu,Daheishan and Naomaohu districts,which are located to the north of the Kalameili Fault.These rocks,sourced from a cognate magma,consist of basic,intermediate,and acidic lavas,and pyroclastic rock.The basic volcanic rocks are enriched with large-ion lithophile elements(LILE),but are relatively depleted in high field strength elements(HFSE),and have an obvious negative Nb-Ta-Ti anomaly.They were most probably derived from a depleted mantle source,and during their ascent,these magmas were not contaminated by the crustal material as they underwent magma crystallization differentiation.Based on the Carboniferous volcanic assemblage and geochemical data,it is apparent that the early Carboniferous volcanism occurred in a subduction-related tectonic setting.New LA-ICP-MS zircon U-Pb analyses constrain the age of the andesite within the volcanic rocks as the early Carboniferous(328.9-331.3 Ma).Combined with the regional geological record,comprehensive analysis of the isotope geochronological data indicates that the subduction of the Junggar Ocean predates the early Carboniferous,and that the Santanghu island arc magmatism was induced by the subduction of the Junggar Ocean in the Carboniferous. | LI Wei LIU YiQun DONG YunPeng ZHOU XiaoHu LIU XiaoMing LI Hong FAN TingTing ZHOU DingWu XU XueYi CHEN JunLu | 2013 | Science China Earth Sciences2013，56，8： | 8 |
| 16 | Texture control by {10-12} twinning to improve the formability of Mg alloys: A review显示文摘Texture control of wrought Mg alloys, particularly in rolled Mg alloy sheets, has been an important research topic for the past several decades because it has significant influence on stretch formability at room temperature. For Mg alloys, {10-12} twinning can be easily activated and causes a 86.3° lattice rotation. Thus, pre-twinning deformation is considered as an effective and low-cost method for texture control in wrought Mg and its alloys. Furthermore, it has been verified that texture control via pre-twinning deformation can remarkably improve stretch formability of rolled Mg alloy sheets. In this review, recent researches on texture control via twinning deformation and its influence on stretch formability will be critically reviewed. The main contents include the micro-mechanism and impact factors of control in twin-orientation, plastic processing techniques of pre-inducing twins and the application of pre-induced twins in improvement of stretch formability. Finally, further research directions on this field were proposed. | Bo Song Qingshan Yang Tao Zhou Linjiang Chai Ning Guo Tingting Liu Shengfeng Guo Renlong Xin | 2019 | Journal of Materials Science & Technology2019，35，10： | 8 |
| 17 | Transcriptome analysis of blood stasis syndrome in subjects with hypertension显示文摘OBJECTIVE:To screen for m RNAs associated with blood stasis syndrome and to explore the genetic mechanisms of blood stasis syndrome in hypertension.METHODS:This study involved groups of patients with hypertension and blood stasis,including those with Qi deficiency,Qi stagnation,cold retention and heat retention;as well as hypertensive patients without blood stasis and healthy individuals.Human umbilical vein endothelial cells were co-cultured with the sera of these healthy individuals and patients with blood stasis syndrome.Total RNA was extracted from these cells and assessed by a high-throughput sequencing method(Solexa)and digital gene expression.Differentially expressed genes among these six groups were compared using whole genome sequences,and m RNAs associated with blood stasis syndrome identified.Differences in gene use and gene ontology function were an-alyzed.Genes enriched significantly and their pathways were determined,as were network interactions,and encoded proteins.Gene identities were confirmed by real-time polymerase chain reactions.RESULTS:Compared with cells cultured in sera of the blood stasis groups,those culture in sera of healthy individuals and of the non-blood stasis group showed 11 and 301 differences,respectively in stasis-related genes.Genes identified as differing between the blood stasis and healthy groups included activating transcription factor 4,activating transcription factor 3,DNA-damage inducible transcription factor 3,Tribbles homolog 3,CCAAT/enhancer binding protein-β,and Jun proto-oncogene(JUN).Pathway and protein interaction network analyses showed that these genes were associated with endoplasmic reticulum stress.Cells cultured in sera of patients with blood stasis and Qi deficiency,Qi stagnation,heat retention,and cold retention were compared with cells cultured in sera of patients with the other types blood stasis syndrome.The comparison showed differences in expression of 28,28,34,and 32 specific genes,respectively.CONCLUSION:The pathogenesis of blood stasis syndrome in hypertension is related to endoplasmic reticulum stress and involves the differential expression of the activating transcription factor 4,activating transcription factor 3,DNA-damage inducible transcription factor 3,Tribbles homolog 3,CCAAT/enhancer binding protein-β,and JUN genes. | He Ling Fang Meixia Chen Liguo Zhou Jianhua Yuan Jing Xu Jing Shan Yan Xu Qingyun Xiong Tingting | 2016 | Journal of Traditional Chinese Medicine2016，36，2： | 7 |
| 18 | Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis显示文摘Lung injury and fibrosis represent the most significant outcomes of severe and acute lung disorders,including COVID-19.However,there are still no effective drugs to treat lung injury and fibrosis.In this study,we report the generation of clinical-grade human embryonic stem cells(hESCs)-derived immunity-and matrix-regulatory cells(IMRCs)produced under good manufacturing practice requirements,that can treat lung injury and fibrosis in vivo.We generate IMRCs by sequentially differentiating hESCs with serum-free reagents.IMRCs possess a unique gene expression profile distinct from that of umbilical cord mesenchymal stem cells(UCMSCs),such as higher expression levels of proliferative,immunomodulatory and anti-fibrotic genes.Moreover,intravenous delivery of IMRCs inhibits both pulmonary inflammation and fibrosis in mouse models of lung injury,and significantly improves the survival rate of the recipient mice in a dose-dependent manner,likely through paracrine regulatory mechanisms.IMRCs are superior to both primary UCMSCs and the FDA-approved drug pirfenidone,with an excellent efficacy and safety profile in mice and monkeys.In light of public health crises involving pneumonia,acute lung injury and acute respiratory distress syndrome,our findings suggest that IMRCs are ready for clinical trials on lung disorders. | Jun Wu Dingyun Song Zhongwen Li Baojie Guo Yani Xiao Wenjing Liu Lingmin Liang Chunjing Feng Tingting Gao Yanxia Chen Ying Li Zai Wang Jianyan Wen Shengnan Yang Peipei Liu Lei Wang Yukai Wang Liang Peng Glyn Nigel Stacey Zheng Hu Guihai Feng Wei Li Yan Huo Ronghua Jin Ng Shyh-Chang Qi Zhou Liu Wang Baoyang Hu Huaping Dai Jie Hao | 2020 | Cell Research2020，30，9： | 7 |
| 19 | A high-fiber diet synergizes with Prevotella copri and exacerbates rheumatoid arthritis显示文摘Both preclinical and established rheumatoid arthritis(RA)patients display alterations in the gut microbiome.Prevotella spp.are preferentially enriched in a subset of RA patients.Here,we isolated a Prevotella strain,P.copri RA,from the feces of RA patients and showed that colonization of P.copri RA exacerbated arthritis in a collagen-induced arthritis(CIA)model.With the presence of P.copri RA colonization,a high-fiber diet exacerbated arthritis via microbial alterations and intestinal inflammation.Colonization of P.copri together with a high-fiber diet enabled the digestion of complex fiber,which led to the overproduction of organic acids,including fumarate,succinate and short-chain fatty acids.Succinate promoted proinflammatory responses in macrophages,and supplementation with succinate exacerbated arthritis in the CIA model.Our findings highlight the importance of dysbiosis when evaluating the effects of dietary interventions on RA pathogenesis and provide new insight into dietary interventions or microbiome modifications to improve RA management. | Lingjuan Jiang Mengmeng Shang Shengnan Yu Yudong Liu Hui Zhang Yangzhong Zhou Min Wang Tingting Wang Hui Li Zhihua Liu Xuan Zhang | 2022 | Cellular & Molecular Immunology2022，19，12： | 6 |
| 20 | Internalization of NK cells into tumor cells requires ezrin and leads to programmed cell-in-cell death显示文摘细胞毒素的淋巴细胞是在有缺点的房间的有免疫力的反应和消除的组织的关键播放器。我们以前报导了生来的杀手(NK ) 房间进入目标肿瘤房间,导致在肿瘤房间以内的任何一个目标房间死亡或自我毁灭。然而,它留下了至于在成为主观以后的 NK 房间的命运逃犯并且 heterotypic cell-in-cell 过程最近是否与同型的 cell-in-cell 事件的不同,说出 entosis。这里,我们证明 NK 房间在肿瘤房间以内与 apoptosis 的最终的命运经历一个 cell-in-cell 过程并且表明成为主观过程要求肌动朊细胞骨架管理者, ezrin。设想 NK 房间怎么进入肿瘤房间,我们执行了 NK 房间成为主观的即时双颜色成像分析进肿瘤房间。令人惊讶地,大多数 NK 房间在他们的入口以后承诺规划房间死亡进肿瘤房间,它与在同型的 cell-in-cell 过程观察的 entosis 区别地不同。使内在化的 NK 房间的 apoptotic 房间死亡由 caspase 的激活是明显的 3 并且 DNA 破碎。而且,在成为主观以后的 NK 房间死亡被 caspase 禁止者稀释, Z-VAD-FMK,在肿瘤房间以内作为 NK 房间死亡的模式证实 apoptosis。决定为 NK 房间的入口必要的蛋白质因素进肿瘤房间,我们执行了基于 siRNA 的击倒的分析并且在 NK 房间成为主观发现了 ezrin 的一个关键角色。重要地, ezrin 的调停 PKA 的 phosphorylation 支持 NK 房间成为主观过程。我们的调查结果建议 ezrin 由管理 NK 房间成为主观进肿瘤房间的新奇规章的机制。 | Shan Wang Zhen Guo Peng Xia Tingting Liu Jufang Wang Shan Li Lihua Sun Jianxin Lu Qian Wen Mingqian Zhou Li Ma Xia Ding Xiaoning Wang Xuebiao Yao | 2009 | Cell Research2009，19，12： | 6 |
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