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| 1 | BMAL1 knockout macaque monkeys display reduced sleep and psychiatric disorders显示文摘Circadian disruption is a risk factor for metabolic, psychiatric and age-related disorders, and non-human primate models could help to develop therapeutic treatments. Here, we report the generation of BMAL1 knockout cynomolgus monkeys for circadian-related disorders by CRISPR/Cas9 editing of monkey embryos. These monkeys showed higher nocturnal locomotion and reduced sleep, which was further exacerbated by a constant light regimen. Physiological circadian disruption was reflected by the markedly dampened and arrhythmic blood hormonal levels. Furthermore, BMAL1-deficient monkeys exhibited anxiety and depression, consistent with their stably elevated blood cortisol, and defective sensory processing in auditory oddball tests found in schizophrenia patients. Ablation of BMAL1 up-regulated transcriptional programs toward inflammatory and stress responses, with transcription networks associated with human sleep deprivation, major depressive disorders, and aging. Thus, BMAL1 knockout monkeys are potentially useful for studying the physiological consequences of circadian disturbance, and for developing therapies for circadian and psychiatric disorders. | Peiyuan Qiu Jian Jiang Zhen Liu Yijun Cai Tao Huang Yan Wang Qiming Liu Yanhong Nie Fang Liu Jiumu Cheng Qing Li Yun-Chi Tang Mu-ming Poo Qiang Sun Hung-Chun Chang | 2019 | National Science Review2019，6，1： | 21 |
| 2 | Comparison of Undernutrition Prevalence of Children under 5 Years in China between 2002 and 2013显示文摘Objective To describe the undernutrition status of children under 5-year in China, and study the trend between 2002 and 2013. Methods The study was based on two national surveys. Undernutrition was determined against WHO's 2006 growth standards. The prevalence in 2013 and 2002 was weighted by China sixth National Population Census(2010). The relationship between undernutrition and gender/age groups/different areas use weighted logistic regression. Results The results indicated the overall prevalence of stunting, underweight, and wasting of Chinese children under 5-year was 8.1%, 2.4%, and 1.9% in 2013, respectively. The prevalence of stunting was higher for children aged 12-47 month, while underweight was higher for children aged 48-59 month. The prevalence of undernutrition was higher in rural areas than in urban areas, especially in poor rural areas. There was a decline of stunting, underweight, and wasting between 2002 and 2013 among the children, with greater reduction in rural areas than in urban areas. Conclusion The prevalence of undernutrition of children under 5-year remains high in rural areas especially in poor rural areas in China. It is urgent to take action to control undernutrition in the vulnerable areas and subgroups. | YU Dong Mei ZHAO Li Yun YANG Zhen Yu CHANG Su Ying YU Wen Tao FANG Hong Yun WANG Xun YU Dan GUO Qi Ya XU Xiao Li FANG Yue Hui ZHAO Wen Hua YANG Xiao Guang DING Gang Qiang LIANG Xiao Feng | 2016 | Biomedical and Environmental Sciences2016，29，3： | 18 |
| 3 | Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer显示文摘Cloning of macaque monkeys by somatic cell nucleus transfer(SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases. Here, we report the feasibility of this approach by SCNT of fibroblasts from a macaque monkey(Macaca fascicularis), in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing(see accompanying paper).Out of 325 SCNT embryos transferred into 65 surrogate monkeys, we cloned five macaque monkeys with BMAL1 mutations in both alleles without mosaicism, with nuclear genes identical to that of the fibroblast donor monkey. Further peripheral blood m RNA analysis confirmed the complete absence of the wild-type BMAL1 transcript. This study demonstrates that the SCNT approach could be used to generate cloned monkeys from fibroblasts of a young adult monkeys and paves the way for the development of macaque monkey disease models with uniform genetic backgrounds. | Zhen Liu Yijun Cai Zhaodi Liao Yuting Xu Yan Wang Zhanyang Wang Xiaoyu Jiang Yuzhuo Li Yong Lu Yanhong Nie Xiaotong Zhang Chunyang Li Xinyan Bian Mu-ming Poo Hung-Chun Chang Qiang Sun | 2019 | National Science Review2019，6，1： | 14 |
| 4 | Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome显示文摘PRKAG2 心脏的症候群正染色体的主导的继承疾病在编码 γ 的 PRKAG2 基因从变化被结果;激活安培的蛋白质 kinase 的 2 规章的子单元。影响病人通常开发室的 tachyarrhythmia 并且经历对医疗倔强并且要求心脏的移植的进步的心失败。在这研究,我们与家庭 Wolff-Parkinson-White 症候群从病人在 PRKAG2 识别一个 H530R 变化。由产生转基因的 H530R PRKAG2 和敲门在里面老鼠,我们证明两个模型包括心脏的肥大和肝糖存储概括人的症状,证实 H530R 变化是有原因地与 PRKAG2 有关心脏的症候群。我们进一步联合联系 adeno 的 virus-9 (AAV9 ) 和 CRISPR/Cas9 编辑基因的系统当让野类型的等位基因未经触动时,破坏编码 H530R 的变异的 PRKAG2 等位基因。在出生后的白天的 AAV9-Cas9/sgRNA 的单个全身的注射 4 或白天 42 实质地在转基因的 H530R PRKAG2 和敲门在里面鼠标恢复心的形态学和函数。一起,我们的工作建议在 vivo CRISPR/Cas9,染色体编辑由有选择地破坏引起疾病的变化是在 PRKAG2 心脏的症候群和另外的主导的继承心脏病的治疗的一个有效工具。 | Chang Xie Ya-Ping Zhang Lu Song Jie Luo Wei Qi Jialu Hu Danbo Lu Zhen Yang Jian Zhang Jian Xiao Bin Zhou Jiu-Lin Du Naihe Jing Yong Liu Yan Wang Bo-Liang Li Bao-Liang Song Yan Yan | 2016 | Cell Research2016，26，10： | 12 |
| 5 | Sequencing of p53 mutation in established human hepatocellular carcinoma cell line of HHC4 and HHC15 in nude mice显示文摘INTRODUCTIONAlthoughtheetiopathologyofhumanhepatocelularcarcinoma(hHCC)isstilunknown,alotofevidencestronglysuggestedthatinfec... | YANG Shan Min, ZHOU Hong, CHEN Rui Chuan, WANG Yu Fang, CHEN Fu, ZHANG Chang Gong, ZHEN Yun, YAN Jiang Hua and SU Jin Hua | 1998 | World Journal of Gastroenterology1998，4，6： | 11 |
| 6 | Quantifying the effects of short-term heat stress at booting stage on nonstructural carbohydrates remobilization in rice显示文摘Extreme heat stress events are becoming more frequent under anticipated climate change,which can have devastating impacts on rice growth and yield.To quantify the effects of short-term heat stress at booting stage on nonstructural carbohydrates(NSC)remobilization in rice,two varieties(Nanjing 41 and Wuyunjing 24)were subjected to 32/22/27°C(maximum/minimum/mean),36/26/31°C,40/30/35°C,and 44/34/39°C for 2,4 and 6 days in phytotrons at booting stage during 2014 and 2015.Yield and yield components,dry matter partitioning index(DMPI),NSC accumulation and translocation were measured and calculated.The results showed that the increase of high-temperature level and duration significantly reduced grain yield by suppressing spikelet number per panicle,seed-setting rate,and grain weight.Heat stress at booting decreased DMPI in panicles,increased DMPI in stems,but had no significant effect on photosynthetic rate.Stem NSC concentration increased whereas panicles NSC concentration,stem NSC translocation efficiency,and contribution of stem NSC to grain yield decreased.Severe heat stress even transformed the stem into a carbohydrate sink during grain filling.The heat-tolerant Wuyunjing 24 showed a higher NSC transport capacity under heat stress than the heat-sensitive Nanjing 41.Heat degree-days(HDD),which combines the effects of the intensity and duration of heat stress,used for quantifying the impacts of heat stress indicates the threshold HDD for the termination of NSC translocation is 9.82°C day.Grain yield was negatively correlated with stem NSC concentration and accumulation at maturity,and yield reduction was tightly related to NSC translocation reduction.The results suggest that heat stress at booting inhibits NSC translocation due to sink size reduction.Therefore,genotypes with higher NSC transport capacity under heat stress could be beneficial for rice yield formation. | Fengxian Zhen Junjie Zhou Aqib Mahmood Wei Wang Xini Chang Bing Liu Leilei Liu Weixing Cao Yan Zhu Liang Tang | 2020 | The Crop Journal2020，8，2： | 9 |
| 7 | Liraglutide reduces the body weight and waist circumference in Chinese overweight and obese type 2 diabetic patients显示文摘目的: 调查 liraglutide 的效果,象 glucagon 一样 peptide-1 (GLP-1 ) 受体使活跃之物,在用超重、肥胖的汉语的身体重量和腰圆周上打 2 个糖尿病的病人。 | Ping FENG De-min YU Li-ming CHEN Bao-cheng CHANG Qiu-di JI Shu-ying LI Mei ZHU Sheng-hua DING Bao- zhen ZHANG Su-li WANG Hong-tao LI Jing-na LIN Mao-jun WANG Jian-chao GUO Jie LIU Zhong-dong LIU Shen- tao WU Ju-hong YANG | 2015 | Acta Pharmacologica Sinica2015，36，2： | 9 |
| 8 | Isolation of 2019-nCoV from a Stool Specimen of a Laboratory- Confirmed Case of the Coronavirus Disease 2019 (COVID-19)显示文摘The novel coronavirus(2019-nCoV)is spreading very fast in Hubei Province of China.As of February 14,2020,51,986 confirmed cases(including laboratory-confirmed cases and clinically-confirmed cases)were reported in Hubei Province,and 1,318 of them died.Respiratory droplets and contact transmission are considered to be the most important routes of transmission of 2019-nCoV,but do not fully account for the occurrence of all coronavirus disease 2019(COVID-19)cases,previously known as novel coronavirus pneumonia(NCP),and the reasons for the rapid spread of this virus(1). | Yong Zhang Cao Chen Shuangli Zhu Chang Shu Dongyan Wang Jingdong Song Yang Song Wei Zhen Zijian Feng Guizhen Wu Jun Xu Wenbo Xu | 2020 | China CDC weekly2020，2，8： | 9 |
| 9 | Metabolomic Analysis of Serum Glycerophospholipid Levels in Eosinophilic and Neutrophilic Asthma显示文摘Objective To compare the serum glycerophospholipid levels in the inflammatory subtypes of asthma by using targeted metabolomic analysis. Methods Demographic and clinical data were collected from 51 patients with asthma between January 2015 and December 2015. Routine blood and sputum induction tests were performed. Eosinophilic asthma was defined as induced sputum containing ≥ 3% eosinophils, and neutrophilic asthma, as induced sputum containing ≥ 71% neutrophils. Serum metabolic glycerophospholipid profile was determined by liquid chromatography-mass spectrometry. Differences in glycerophospholipid levels between eosinophilic and non-eosinophilic asthma and between neutrophilic and non-neutrophilic asthma were analyzed using partial least squares discriminant analysis. Results The serum lysophosphatidylglycerol level was significantly higher in the group with ≥ 3% eosinophils in sputum than in the group with < 3% eosinophils in sputum. The area under the receiver-operating characteristic curve was ≥ 70%. There was no significant difference in the serum metabolic glycerophospholipid profile between the group with sputum neutrophils ≥ 71% and the group with sputum neutrophils < 71%. Conclusion Serum lysophosphatidylglycerol is produced abundantly in eosinophilic asthma and may be a biomarker of eosinophilic asthma. This information is helpful for identifying and tailoring treatment for the common asthma subtypes. | GAI Xiao Yan ZHANG Li Jiao CHANG Chun GUO Cheng Lin ABULIKEMU Mairipaiti LI Wen Xiong WANG Juan YAO Wan Zhen ZHANG Xu | 2019 | Biomedical and Environmental Sciences2019，32，2： | 8 |
| 10 | Malnutrition and Quality of Life in Chinese Cancer Patients: a Clinical Study of 23,994 Subjects显示文摘Background Malnutrition is common in patients with cancer,and can negatively impact their quality of life(QoL)and even survival.However,there is currently no large data available on the prevalence of malnutrition in Chinese cancer patients.This study evaluated the prevalence of malnutrition and the QoL of Chinese patients with locoregional,recurrent or metastatic cancer.Methods We conducted a nationwide observational,multi-center,hospital-based cross-sectional study within the Chinese Society of Nutritional Oncology(CSNO)Network.All of the patients were diagnosed with one of the following 18 different types of malignant tumors:lung cancer,gastric cancer,liver cancer,colorectal cancer,breast cancer,esophageal cancer,cervical cancer,endometrial cancer,nasopharyngeal carcinoma,malignant lymphoma,leukemia,pancreatic cancer,ovarian cancer,prostate cancer,bladder cancer,brain cancer,biliary tract malignant tumors or gastrointestinal stromal tumors.These patients were enrolled from 72 hospitals located in different regions of China.The patients’nutritional status was evaluated based on the body mass index(BMI),loss of bodyweight,laboratory measurements and patient generated-subjective global assessment(PG-SGA)scores.The cancer patients’physical status and QoL were assessed by the Karnofsky Performance Status(KPS)questionnaire and the European Organization for Research and Treatment of Cancer(EORTC)QLQ-C30 questionnaire,respectively.Results From December 2013 to April 2016,23,994 patients hospitalized for cancer treatment(such as surgery,chemotherapy or radiotherapy)were enrolled in the study.The patients included 12,494(52.9%)males and 11,124(47.1%)females.The mean age was 55.8±13.7 years.The proportions of patients in cancer stagesⅠ,Ⅱ,Ⅲ,Ⅳand uncertain were 11.5%,20.3%,27.5%,30.2%and 10.5%,respectively.Among the 23,994 inpatients,the proportions of patients who were underweight(BMI<18.5 kg/m2),normal(18.5 kg/m2 | Hong Xia Xu Chun Hua Song Zhen Ming Fu Chang Wang Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Jiu Wei Cui Wei Li Han Ping Shi the Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2021 | Journal of Nutritional Oncology2021，6，1： | 8 |
| 11 | Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma显示文摘AIM:To clarify the specific roles and mechanisms of long interspersed nuclear element-1 ORF-1 protein [human long interspersed nuclear element-1(LINE-1),ORF-1p] in chemotherapeutic drug resistance and cell proliferation regulation in hepatocellular carcinoma(HCC) cells.METHODS:MTT assays were performed to identify the effect of the chemotherapeutic drug toxicity on HepG2 cells.Cell proliferation inhibition and the IC 50 were calculated by the Origin 8.0 software.Western blotting assays were performed to investigate whether LINE-1 ORF-1p modulates the expression of some important genes,including p53,p27,p15,Bcl-2,mdr,and p-gp.To corroborate the proliferation and anchor-independent growth results,the HepG2 cells were analyzed by flow cytometry to investigate the effect of LINE-1 ORF1p on the apoptosis regulation.RESULTS:LINE-1 ORF-1p contributed to the resistance to several chemotherapeutic drugs(cisplatin and epirubicin) in HepG2 cells.The IC 50 of the epirubicin and cisplatin increased from 36.04 nmol/L to 59.11 nmol/L or from 37.94 nmol/L to 119.32 nmol/L.Repression of LINE-1 ORF-1p expression by the siRNA could markedly enhance the response of HepG2 cells to the epirubicin and cisplatin.The IC 50 correspondingly decreased from 28.06 nmol/L to 3.83 nmol/L or from 32.04 nmol/L to 2.89 nmol/L.Interestingly,down-regulation of LINE-1 ORF-1p level by siRNA could promote the response of HepG2 cells to the paclitaxel.The IC 50 decreased from 35.90 nmol/L to 7.36 nmol/L.However,overexpression of LINE-1 ORF-1p did not modulate the paclitaxel toxicity in HepG2 cells.Further Western blotting revealed that LINE-1 ORF-1p enhanced mdr and p-gp gene expression.As a protein arrested in the nucleus,LINE-1 ORF-1p may function through modulating transcriptional activity of some important transcription factors.Indeed,LINE-1 ORF-1p promoted HepG2 cell proliferation,anchor-independent growth and protected the cells against apoptosis through modulating the expression of p15,p21,p53,and Bcl-2 genes.CONCLUSION:LINE-1 ORF-1p promotes HepG2 cell proliferation and plays an important role in the resistance of chemotherapeutic drugs.By establishing novel roles and defining the mechanisms of LINE-1 ORF1p in HCC chemotherapeutic drug resistance and cell proliferation regulation,this study indicates that LINE-1 ORF-1p is a potential target for overcoming HCC chemotherapeutic resistance. | Fan Feng Yin-Ying Lu Fan Zhang Xu-Dong Gao Chuan-Fu Zhang Alex Meredith Zhong-Xian Xu Yu-Tao Yang Xiu-Juan Chang Hong Wang Jian-Hui Qu Zhen Zeng Jun-Lan Yang Chun-Ping Wang Yun-Feng Zhu Jia-Jun Cui Yong-Ping Yang | 2013 | World Journal of Gastroenterology2013，19，7： | 8 |
| 12 | Prognostic value of lymph node metastasis in patients with T1-stage colorectal cancer from multiple centers in China显示文摘AIM To explore the features and prognostic value of lymph node metastasis in patients with T1-stage colorectal cancer(CRC).METHODS In all,321 cases of T1-stage CRC were selected from 10132 patients with CRC who received surgical therapy in six large-scale hospitals in China and were retrospectively analyzed. Univariate and multivariate analyses were performed to analyze the risk factors for lymphatic metastasis. A survival analysis was then performed to analyze the prognostic value of lymph node metastasis.RESULTS The occurrence rate of T1 stage was 3.17%(321/10132);of these patients,the lymph node metastasis rate was 8.41%(27/321),and the non-lymph node metastasis rate was 91.59%(294/321). Univariate analysis showed that preoperative serum CEA,preoperative serum CA199,preoperative serum CA724,vascular invasion,and degree of differentiation were associated with lymph node metastasis in T1-stage CRC(P < 0.05 for all). Multivariate analysis indicated that preoperative serum CA724,vascular invasion,and degree of differentiation were closely related to lymph node metastasis(P < 0.05 for all). Log-rank survival analysis showed that age,preoperative serum CEA,preoperative serum CA199,vascular invasion,degree of differentiation,and lymph node metastasis(χ2 = 24.180,P < 0.001) were predictors of 5-year overall survival(OS)(P < 0.05 for all). COX regression analysis demonstrated that preoperative serum CA199 and lymph node metastasis(HR = 5.117;P < 0.05;95%CI: 0.058-0.815) were independent prognostic indicators of 5-year OS in patients with T1-stage CRC(P < 0.05 for both). CONCLUSION The morbidity of T1-stage CRC was 3.17% for all CRC cases. Preoperative serum CA724,vascular invasion,and degree of differentiation are independent risk factors for lymph node metastasis. Lymph node metastasis is an independent prognostic factor for OS in patients with T1-stage CRC. | Zhen-Qiang Sun Shuai Ma Quan-Bo Zhou Shuai-Xi Yang Yuan Chang Xiang-Yue Zeng Wei-Guo Ren Fang-Hai Han Xiang Xie Fan-Ye Zeng Xian-Tao Sun Gui-Xian Wang Zhen Li Zhi-Yong Zhang Jun-Min Song Jin-Bo Liu Wei-Tang Yuan | 2017 | World Journal of Gastroenterology2017，23，48： | 7 |
| 13 | Cryotherapy for cirrhosis-based hepatocellular carcinoma: a single center experience from 1595 treated cases显示文摘Cryoablation 是为 hepatocellular 癌(HCC ) 的不太流行的经皮的夺格的治疗,并且关于它的实用性的当前的证据是有限的。我们在与经皮的 cryoablation 对待 1595 个 HCC 案例关于有效性,安全和这治疗的长期的结果给全面侧面报导我们的经验。从 2003 年 1 月到 2013 年 12 月,有 2313 个 HCC 小瘤的 1595 个病人在我们的中心与 2958 个 cryoablation 会议被切除。完全的脱离与 3.4 朠湥浯捩椠獮慴楢楬祴 ? 畢 ? 的一条吝啬的直径为 1893 个小瘤在 1294 个病人被完成 ?? 潣扭湩摥眠瑩 ??? 瑡整畮瑡摥琠敨攠瑸湥 ? 景朠湥浯捩椠獮慴楢楬祴椠 ? 牰浩牡 ? 塖′畴潭 ? 楴獳敵瑡吗?? | Guanghua Rong Wenlin Bai Zheng Dong Chunping Wang Yinying Lu Zhen Zeng Jianhui Qu Min Lou Hong Wang Xudong Gao Xiujuan Chang Linjing An Yan Chen Yongping Yang | 2015 | Frontiers of Medicine2015，9，1： | 7 |
| 14 | Validity of the Chinese Version of the Patient-Generated Subjective Global Assessment (PG-SGA) in Lung Cancer Patients显示文摘The prevalence of malnutrition is high among oncology patients in China. Although the Patient- Generated Subjective Global Assessment (PG-SGA) is widely recognized as an important nutritional assessment tool, it has not been validated for Chinese cancer patients. The purpose of this study was to conduct an analysis of nutrition screening and assessment data to validate the Chinese version of the PG-SGA in Chinese patients with lung cancer. This was an observational, cross-sectional study of 2,000 consecutive adult patients with lung cancer treated at several tertiary hospitals in China. Anthropometric and patient descriptive data were collected. The PG-SGA generated a score for the nutritional risk and a global rating for the nutritional status. The internal consistency, external consistency, and construct validity were evaluated, and a known-groups comparison and principal component analyses were conducted to evaluate the reliability and validity of the PG-SGA. The known-groups comparison demonstrated the ability of the PG-SGA to differentiate lung cancer patients of different nutritional and body mass index (BMI) groups. A confirmatory factor analysis supported the original four-factor structure of the PG-SGA. In terms of the internal and external consistency, the PG-SGA demonstrated intra-class correlation coefficients (ICC) up to 0.987. Alternative form validity was also demonstrated between Box 4 of the PG-SGA and the Karnofsky Performance Status score, with excellent external consistency (r = 0.972, P < 0.001). The construct validity was supported, and selected questionnaire dimensions were evident in the principal component analysis. Significant Spearman correlations (P < 0.001) were demonstrated. The Chinese version of the PG-SGA demonstrated significant reliability and sufficient exploratory properties to support its validity. It seems to be a valid tool that can be used to access the nutritional status of Chinese lung cancer patients. The validity of the PG-SGA in Chinese cancer patients warrants further investigation in other cancer types. | Zhen Ming Fu Hong Xia Xu Chun Hua Song Wei Li Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Chang Wang Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) Group | 2016 | Journal of Nutritional Oncology2016，1，1： | 7 |
| 15 | Effect of Simulated Microgravity and its Associated Mechanism on Pulmonary Circulation in Rats显示文摘Objective To study the effect of Simulated Microgravity and its Associated Mechanism on Pulmonary Circulation in Rats. Methods Rat tail-suspension model was used to simulate the physiological effects of microgravity and changes in pulmonary blood vessel morphology, pulmonary arterial and venous blood pressure, pulmonary vascular resistance, pulmonary vasomotoricity, as well as the regulation of pulmonary circulation by cytokines produced and released by the lung of rats were measured. Results The walls of pulmonary blood vessels of rats were thickened, and the pulmonary artery was reconstructed with increased pulmonary vascular resistance. The pulmonary blood vessels of rats became more prone to dilation as contractions increased. Rat epithelial Adrenomedulin gene transcription and protein expression were upregulated. The level of basic fibroblast growth Factor of rat was also elevated. Conclusion Findings from the present study on rats revealed that the microgravity can affect pulmonary blood vessel structure, pulmonary arterial pressure, and pulmonary blood vessel self-regulation and cytokine production. | LI Tian Zhi YUAN Ming CHEN Zhen Hong GUO Ying Hua KANG Chun Yan WANG Jing Yu LI Zhi Li WANG De Sheng WANG Hui Juan YUAN Min LIU Chang Ting | 2013 | Biomedical and Environmental Sciences2013，26，2： | 6 |
| 16 | De novo assembly and comparative analysis of root transcriptomes from different varieties of Panax ginseng C. A. Meyer grown in different environments显示文摘Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at least the Ming dynasty to increase production, cultivated ginseng has lower quantities of ginsenosides and lower disease resistance than ginseng grown under natural conditions. We extracted root RNA from six varieties of fifth-year P. ginseng cultivars representing four different growth conditions, and performed Illumina paired-end sequencing. In total, 163,165,706 raw reads were obtained and used to generate a de novo transcriptome that consisted of 151,763 contigs(76,336 unigenes), of which 100,648 contigs(66.3%) were successfully annotated. Differential expression analysis revealed that most differentially expressed genes(DEGs) were upregulated(246 out of 258, 95.3%) in ginseng grown under natural conditions compared with that grown under artificial conditions. These DEGs were enriched in gene ontology(GO) terms including response to stimuli and localization. In particular, some key ginsenoside biosynthesis-related genes, including HMG-Co A synthase(HMGS), mevalonate kinase(MVK), and squalene epoxidase(SE), were upregulated in wild-grown ginseng. Moreover, a high proportion of disease resistance-related genes were upregulated in wild-grown ginseng. This study is the first transcriptome analysis to compare wild-grown and cultivated ginseng, and identifies genes that may produce higher ginsenoside content and better disease resistance in the wild; these genes may have the potential to improve cultivated ginseng grown in artificial environments. | ZHEN Gang ZHANG Lei DU YaNan YU RenBo LIU XinMin CAO FangRui CHANG Qi DENG Xing Wang XIA Mian HE Hang | 2015 | Science China(Life Sciences)2015，58，11： | 6 |
| 17 | Overexpression of metastasis-associated in colon cancer 1 predicts a poor outcome of hepatitis B virus-related hepatocellular carcinoma显示文摘AIM: To investigate the intratumoral expression of metastasis-associated in colon cancer 1 (MACC1) and c-Met and determine their clinical values associated with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). METHODS: A retrospective study admitted three hundred fifty-four patients with HBV-related HCC. The expression and distribution of MACC1 and c-Met were assessed by quantitative real-time polymerase chain reaction and immunohistochemistry staining. Prognostic factors influencing survival, metastasis and recurrence were assessed. RESULTS: Intratumoral MACC1 level was found to be associated with HCC disease progression. Both median tumor-free survival (TFS) and overall survival (OS) were significantly shorter in the postoperative HCC patients with high intratumoral MACC1 expression, as compared to those with low intratumoral MACC1 levels (TFS: 34 mo vs 48.0 mo, P < 0.001; OS: 40 mo vs 48 mo, P < 0.01). Multivariable analysis indicated that high MACC1 expression or co-expression with c-Met were independent predictors for HCC clinic outcome (P < 0.001). CONCLUSION: High intratumoral MACC1 expression can be associated with enhanced tumor progression and poor outcome of HBV-related HCC. MACC1 may serve as a prognostic biomarker for postoperative HCC. | Jian-Hui Qu Xiu-Juan Chang Yin-Ying Lu Wen-Lin Bai Yan Chen Lin Zhou Zhen Zeng Chun-Ping Wang Lin-Jing An Li-Yan Hao Gui-Lin Xu Xu-Dong Gao Min Lou Ji-Yun Lv Yong-Ping Yang | 2012 | World Journal of Gastroenterology2012，18，23： | 6 |
| 18 | Validity of the Chinese Version of the Patient-Generated Subjective Global Assessment (PG-SGA) in Gastric Cancer Patients显示文摘Purpose Malnutrition is severe among gastric cancer patients in China. While the Patient-Generated Subjective Global Assessment (PG-SGA) is a widely used nutritional assessment tool, the validation for Chinese patients with gastric cancer has not been performed yet. The aim of this research is to validate the Chinese version of the PGSGA in Chinese gastric cancer patients by conducting analyses of nutrition screening and assessment data. Methods Two thousand consecutive adult patients with gastric cancer were included in this observational, cross-sectional study in several tertiary hospitals in China. A survey was made among those patients, which included several questionnaires and some anthropometric parameters. We calculated the parameters to validate the use of PG-SGA categorizing the nutritional status of gastric cancer patients. Then we performed statistical analysis to evaluate the internal consistency, external consistency, and construct validity. Results This study verified the ability of the PG-SGA to differentiate gastric cancer patients into different nutritional groups. The confirmatory factor analysis verified the original four-factor structure of the PG-SGA. The internal and external consistency was measured by a concordance analysis which showed an intraclass correlation coefficients (ICC) of 0.987. The correlations of the Box 4 score with the Karnofsky performance status score demonstrated alternative form validity with good external consistency (Pearson, r = 0.643, P < 0.001). All three anthropometric dimensions of the PG-SGA were supported by principal component analysis. Spearman correlations (P < 0.001) were significant in the majority of tests. Conclusion The Chinese version of the PG-SGA is a valid tool to access the patients’ nutritional status for Chinese gastric patients. | Zhen Ming Fu Hong Xia Xu Chun Hua Song Wei Li Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Chang Wang Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) Group | 2018 | Journal of Nutritional Oncology2018，3，4： | 5 |
| 19 | Differentially expressed microRNAs at different stages of atherosclerosis in ApoE-deficient mice显示文摘 | SHAN Zhen YAO Chen LI Zi-lun TENG Yuan LI Wen WANG Jin-song YE Cai-sheng CHANG Guang-qi HUANG Xue-ling LI Xiao-xi WANG Wen-jian WANG Shen-ming | 2013 | Chinese Medical Journal2013，，3： | 5 |
| 20 | Perihematomal endothelin-1 level is associated with an increase in blood-brain barrier permeability in a rabbit model of intracerebral hematoma显示文摘 | WANG Li-kun HONG Zhen WU Guo-feng LI Chang | 2013 | Chinese Medical Journal2013，，18： | 5 |
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