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| 1 | Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes显示文摘在从经由单性生殖和男性单性生殖的老鼠的胚胎的干细胞(haESCs ) 启用了在修改基因的动物的哺乳动物的房间和代的基因屏蔽的 haploid 的推导的最近的成功。然而, haESCs 是否能从首领被导出,仍然保持未知。这里,我们从 Macaca fascicularis 猴子的单性生殖的胚囊报导 haESCs 的推导。这些房间,作为 PG-haESCs 称为,是 pluripotent 并且能在 vitro 或在 vivo 区分到三胚胎的细菌层的房间。有趣地,一根猴子 PG-haESC 线(MPH1 ) 的 haploidy 与另一个(MPH2 ) 的相比是更稳定的,作为没有排序的激活荧光的房间(FACS ) ,由 haploid 房间的存在出现超过 140 天 haploid 房间的丰富。重要地,转基因的猴子 PG-haESC 线能被 lentivirus- 和 piggyBac 产生调停 transposon 的基因转移。而且,基因屏蔽在猴子 PG-haESCs 是可行的。我们的结果证明 PG-haESCs 能从猴子被产生,提供为在首领的基因分析的一个理想的工具。 | Hui Yang Zhen Liu Yu Ma Cuiqing Zhong Qi Yin Chikai Zhou Linyu Shi Yijun Cai Hanzhi Zhao Hui Wang Fan Tang Yan Wang Chenchen Zhang Xin-yuan Liu Dongmei Lai Ying Jin Qiang Sun Jinsong Li | 2013 | Cell Research2013，23，10： | 22 |
| 2 | BMAL1 knockout macaque monkeys display reduced sleep and psychiatric disorders显示文摘Circadian disruption is a risk factor for metabolic, psychiatric and age-related disorders, and non-human primate models could help to develop therapeutic treatments. Here, we report the generation of BMAL1 knockout cynomolgus monkeys for circadian-related disorders by CRISPR/Cas9 editing of monkey embryos. These monkeys showed higher nocturnal locomotion and reduced sleep, which was further exacerbated by a constant light regimen. Physiological circadian disruption was reflected by the markedly dampened and arrhythmic blood hormonal levels. Furthermore, BMAL1-deficient monkeys exhibited anxiety and depression, consistent with their stably elevated blood cortisol, and defective sensory processing in auditory oddball tests found in schizophrenia patients. Ablation of BMAL1 up-regulated transcriptional programs toward inflammatory and stress responses, with transcription networks associated with human sleep deprivation, major depressive disorders, and aging. Thus, BMAL1 knockout monkeys are potentially useful for studying the physiological consequences of circadian disturbance, and for developing therapies for circadian and psychiatric disorders. | Peiyuan Qiu Jian Jiang Zhen Liu Yijun Cai Tao Huang Yan Wang Qiming Liu Yanhong Nie Fang Liu Jiumu Cheng Qing Li Yun-Chi Tang Mu-ming Poo Qiang Sun Hung-Chun Chang | 2019 | National Science Review2019，6，1： | 21 |
| 3 | Isoliquiritigenin inhibits TGF-β1-induced fibrogenesis through activating autophagy via PI3K/AKT/mTOR pathway in MRC-5 cells显示文摘Isoliquiritigenin(ISL),a natural flavonoid derived from the root of liquorice,has been reported to possess anti-inflammatory and antioxidant activities.Previous studies have found that ISL plays a crucial role in anti-fibrosis of adipose tissue and renal tissue;however,its effect on pulmonary fibrogenesis has not been demonstrated.In this study,we aimed to explore the roles and the underlying mechanisms of ISL in TGF-β1-induced fibrogenesis using human lung fibroblast-derived MRC-5 cells.Cell proliferation and migration were determined by MTT and wound healing assay,respectively.The expression levels of alpha-smooth muscle actin(α-SMA),collagen type I alpha 1(COLIA1)and fibronectin(FN),microtubule-associated protein light chain 3(LC3)and related signaling molecules were detected by quantitative real-time PCR,western blot and immunofluorescence assay,correspondingly.EGFP-LC3 transfection was used for autophagy analysis.The results showed that ISL inhibited the TGF-β1-induced proliferation and migration,and down-regulated the expressions ofα-SMA,COLIA1 and FN.ISL treatment led to up-regulation of LC3 in TGF-β1-treated MRC-5 cells,accompanied by significant decrease in the phosphorylation levels of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),and mammalian target of rapamycin(mTOR).In addition,the inhibitory effects of ISL on TGF-β1-induced fibrogenic features in MRC-5 cells were enhanced by pretreatment with autophagy activator Rapmycin and PI3K/AKT inhibitor LY294002 and reversed by autophagy inhibitor 3-methyladenine and PI3K/AKT activator IGF-1.Taken together,our results demonstrated that ISL could attenuate the fibrogenesis of TGF-β1-treated MRC-5 cells by activating autophagy via suppressing the PI3K/AKT/mTOR pathway.Therefore,ISL holds a great potential to be developed as a novel therapeutic agent for the treatment of pulmonary fibrosis. | Jinjuan He Hao Peng Meifang Wang Ying Liu Xingrong Guo Bin Wang Longjun Dai Xueqin Cheng Zhongji Meng Leyong Yuan Fenglin Cai Yijun Tang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，8： | 16 |
| 4 | Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer显示文摘Cloning of macaque monkeys by somatic cell nucleus transfer(SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases. Here, we report the feasibility of this approach by SCNT of fibroblasts from a macaque monkey(Macaca fascicularis), in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing(see accompanying paper).Out of 325 SCNT embryos transferred into 65 surrogate monkeys, we cloned five macaque monkeys with BMAL1 mutations in both alleles without mosaicism, with nuclear genes identical to that of the fibroblast donor monkey. Further peripheral blood m RNA analysis confirmed the complete absence of the wild-type BMAL1 transcript. This study demonstrates that the SCNT approach could be used to generate cloned monkeys from fibroblasts of a young adult monkeys and paves the way for the development of macaque monkey disease models with uniform genetic backgrounds. | Zhen Liu Yijun Cai Zhaodi Liao Yuting Xu Yan Wang Zhanyang Wang Xiaoyu Jiang Yuzhuo Li Yong Lu Yanhong Nie Xiaotong Zhang Chunyang Li Xinyan Bian Mu-ming Poo Hung-Chun Chang Qiang Sun | 2019 | National Science Review2019，6，1： | 14 |
| 5 | Review of Chinese atmospheric science research over the past 70 years: Atmospheric physics and atmospheric environment显示文摘Since the founding of the People’s Republic of China 70 years ago,the subject of atmospheric physics and atmospheric environment has developed rapidly in China,providing important support for the development of atmospheric science and guarantee for the development of national economy.In this paper,the general advancement of atmospheric physics and atmospheric environment in last 70 years was described.The main research progress of atmospheric physics and atmospheric environment in the past 40 years of reform and opening-up was reviewed,the outstanding research achievements since the 21 st century were summarized,the major problems and challenges are pointed out,and the key directions and suggestions for future development are put forward. | Tijian WANG Taichang GAO Hongsheng ZHANG Maofa GE Hengchi LEI Peichang ZHANG Peng ZHANG Chunsong LU Chao LIU Hua ZHANG Qiang ZHANG Hong LIAO Haidong KAN Zhaozhong FENG Yijun ZHANG Xiushu QIE Xuhui CAI Mengmeng LI Lei LIU Shengrui TONG | 2019 | Science China Earth Sciences2019，62，12： | 5 |
| 6 | Physicochemical and tribological properties of gemini-type halogen-free dicationic ionic liquids显示文摘A series of new halogen-free dicationic ionic liquids(ILs)with different alkyl chain lengths were prepared,and the relationship between the alkyl chain length,physicochemical and tribological properties of ILs,and their role as neat lubricant for steel–steel friction pairs,was investigated.Evaluation of stability during hydrolysis and copper strip corrosion test results show that synthetic ILs are stable and not corrosive to metal contacts,due to the halogen-free anions.The friction and wear test results indicate that ILs with long alkyl chains have excellent friction-reducing and anti-wear properties,especially at high temperatures.Based on the surface three-dimensional(3D)profiles,electrical contact resistance,scanning electron microscopy(SEM),energy dispersive X-ray spectroscopy(EDS),and the X-ray photoelectron spectrometry(XPS)analysis of the worn surfaces of steel discs,we can conclude that the efficiency of ILs is due to the formation of high quality tribofilms that consist of both tribochemical reaction and ordered absorption films. | Qiangliang YU Chaoyang ZHANG Rui DONG Yijun SHI Yurong WANG Yanyan BAI Jiaying ZHANG Meirong CAI Feng ZHOU Weimin LIU | 2021 | Friction2021，9，2： | 3 |
| 7 | Accelerated passage of gene-modified monkeys by hormone-induced precocious puberty显示文摘Mosaicism occurs in most founder genemodified monkeys generated by virus infection and programmable-nucleases[1-3]. In addition, macaque monkeys have a long preadolescence period, with four or more years usually required for male macaque monkeys to become sexually mature [4,5]. The mosaicism and long time to sexual maturity have largely prevented wide usage of gene-modified monkey models. | Zhen Liu Kui Li Yijun Cai Qiming Liu Tikui Zhang Yong Lu Yanhong Nie Yan Wang Zhiguang Yan Yinwei Qu Yuzhuo Li Zhanyang Wang Zhi-Qi Xiong Qiang Sun | 2021 | National Science Review2021，8，7： | 2 |
| 8 | Sparse representation of global features of visual images in human primary visual cortex: Evidence from fMRI显示文摘In fMRI experiments on object representation in visual cortex, we designed two types of stimuli: one is the gray face image and its line drawing, and the other is the illusion and its corresponding completed illusion. Both of them have the same global features with different minute details so that the results of fMRI experiments can be compared with each other. The first kind of visual stimuli was used in a block design fMRI experiment, and the second was used in an event-related fMRI experiment. Comparing and analyzing interesting visual cortex activity patterns and blood oxygenation level dependent (BOLD)- fMRI signal, we obtained results to show some invariance of global features of visual images. A plau- sible explanation about the invariant mechanism is related with the cooperation of synchronized re- sponse to the global features of the visual image with a feedback of shape perception from higher cortex to cortex V1, namely the integration of global features and embodiment of sparse representation and distributed population code. | ZHAO SongNian YAO Li JIN Zhen XIONG XiaoYun WU Xia ZOU Qi YAO GuoZheng CAI XiaoHong LIU YiJun | 2008 | Chinese Science Bulletin2008，53，14： | 2 |
| 9 | An Experimental and Kinetic Modelling Study of Three Butene Isomers Pyrolysis at Low Pressure 显示文摘 | ZHANG Yijun CAI Jianghuai1 ZHAO Long | 2012 | Combustion and Flame2012，159，3： | 1 |
| 10 | Enmein-type diterpenoid analogs from natural kaurene-type oridonin: Synthesis and their antitumor biological evaluation显示文摘 | Dahong Li Shengtao Xu Hao Cai Lingling Pei Hengyuan Zhang Lei Wang Hequan Yao Xiaoming Wu Jieyun Jiang Yijun Sun Jinyi Xu | 2013 | European Journal of Medicinal Chemistry2013，，： | 1 |
| 11 | Singularity treatment and high-order RWG basis functions for integral equations of electromagnetic scattering显示文摘 | Cai W Yu Yijun Yuan X C | | 0，，1： | 1 |
| 12 | Poly ( styrene-ah-maleic anhydride) derivatives as potent anti-HIV mierobicide candidates 显示文摘 | Fang Weijun Cai Yijun Chen Xiaoping | 2009 | Bioorganic & Medicinal Chemistry Letters2009，19，： | 1 |
| 13 | Background progenitor activation is associated with recurrence after hepatectomy of combined hepatocellular‐cholangiocarcinoma显示文摘 | Xiong Cai Jian Zhai David E. Kaplan Yijun Zhang Lining Zhou Xutao Chen Guangyang Qian Qiudong Zhao Yonghai Li Lu Gao Wenming Cong Minghua Zhu Zhenlin Yan Lehua Shi Dong Wu Lixin Wei Feng Shen Mengchao Wu | 2012 | Hepatology2012，，5： | 1 |
| 14 | Design, Synthesis and Biological Evaluation of Novel Selective Thiol-based Histone Deacetylase(HDAC) Ⅵ Inhibitors Bearing Indeno[1,2-c]pyrazole or Benzoindazole Scaffold显示文摘 | XU Qihao YU Shujia CAI Yijun YANG Jinyu ZHAO Linxiang LIU Dan | 2018 | Chemical Research in Chinese Universities2018，34，1： | 0 |
| 15 | Hawking Evaporation for the 2+1 Dimensional Radiating Black Hole显示文摘By using a metric which is a counterpart of four dimensional Vaidya metric,we have investigated the Hawking evaporation of the 2+1 dimensional radiating black hole.The Hawking temperature of this radiating black hole is given.In a specific case,our result reduces to that of Banados.Teitelboim and Zanelli’s. | CAI Ronggen ZHANG Yijun SU Rukeng | 1995 | Chinese Physics Letters1995，12，7： | 0 |
| 16 | Differences between triggered lightning striking to ground and distribution line inferred from measured currents and electromagnetic fields显示文摘In 2018-2019,two types of rocket-triggered lightning tests,that is,striking to ground and distribution line,were conducted in Guangdong,China.The main focus of this paper is to compare the waveform parameters of the return strokes(RS)from the two tests.For current,the 10%-90%risetime of RS from lightning striking to distribution line is 2.4 times higher than that striking to ground,and the differences in the other parameters are small.For close magnetic field,the RS from lightning striking to distribution line have a lower peak(30%lower after considering the peak current)and a larger risetime and half-peak width time;besides,it is necessary to divide the front edge of the close magnetic field waveform into two parts:leader(BL)and return stroke(BRS).For close electric field,the close RS electric field peak(ERS)was less sensitive to distance than the leader electric field peak(EL).For far electric field,the differences in waveform parameters of RS from the two tests were not significant,but after considering the peak current,the normalised initial electric field peak(EP)of RS from lightning striking to distribution line was 14%lower than that striking to ground. | Li Cai Qiang Hu Jianguo Wang Mi Zhou Quanxin Li Rui Su Yijun Huang | 2022 | High Voltage2022，7，4： | 0 |
| 17 | 利用体细胞核移植技术构建克隆猕猴显示文摘文章简介体细胞核移植,又称体细胞克隆,即将体细胞注入去核的卵母细胞中,使体细胞在卵母细胞中重编程并发育为新的胚胎,进而获得动物个体。1997年,克隆羊'Dolly'诞生,之后许多其他体细胞克隆哺乳动物如雨后春笋般被相继报道出来。 | Zhen Liu Yijun Cai Yan Wang Yanhong Nie Chenchen Zhang Yuting Xu Xiaotong Zhang Yong Lu Zhanyang Wang Muming Poo 孙强 | 2019 | 科学新闻2019，0，2： | 0 |
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