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| 1 | miRNA-711-SP1-collagen-I pathway is involved in the anti-fibrotic effect of pioglitazone in myocardial infarction显示文摘Although microRNAs(miRNAs) have been intensively studied in cardiac fibrosis,their roles in drug-mediated anti-fibrotic therapy are still unknown.Previously,Pioglitazone attenuated cardiac fibrosis and increased miR-711 experimentally.We aimed to explore the role and mechanism of miR-711 in pioglitazone-treated myocardial infarction in rats.Our results showed that pioglitazone significantly reduced collagen-I levels and increased miR-711 expression in myocardial infarction heart.Pioglitazone increased the expression of miR-711 in cardiac fibroblasts,and overexpression of miR-711 suppressed collagen-I levels in angiotensin II(Ang II)-treated or untreated cells.Transfection with antagomir-711 correspondingly abolished the pioglitazone-induced reduction in collagen-I levels.Bioinformatics analysis identified SP1,which directly promotes collagen-I synthesis,as the putative target of miR-711.This was confirmed by luciferase assay and western blot analysis.Additionally,increased SP1 expression was attenuated by pioglitazone in myocardial infarction heart.Furthermore,transfection of antagomir-711 attenuated pioglitazone-reduced SP1 expression in cardiac fibroblasts with or without Ang II stimulation.We conclude that pioglitazone up-regulated miR-711 to reduce collagen-I levels in rats with myocardial infarction.The miR-711-SP1-collagen-I pathway may be involved in the anti-fibrotic effects of pioglitazone.Our findings may provide new strategies for miRNA-based anti-fibrotic drug research. | ZHAO Na YU HaiYi YU HaiTao SUN Min ZHANG YouYi XU Ming GAO Wei | 2013 | Science China(Life Sciences)2013，56，5： | 9 |
| 2 | Triassic Transgressive-Regressive Sequences in Guizhou-Guangxi Region, South China显示文摘Seven third-order transgressive-regressive sequences are recognized in the Early Triassic -Early Carnian stratigraghc succession of Guizhou-Guangxi region, South China. Based on currently available ages by the correlation of conodont ammonites and bivalve faunas, these sequences range approximately from 1.0 Ma to 5.6 Ma, with the average of 3 Ma. They occur largely in the Griesbachian-Dienerian, Smithian,uppermost Smithian-lowermost Anisian, Lower Anisian, Middle Anisian, Upper Anisian-Upper Ladinian and the uppermost Ladinian-Lower Carnian respectively, and can be grouped into three second-order transgressive-regressive supersequences. A relative sea level curve is established from the sequence stratigraghic analysis. Transgressive-regressive sequences,bounded by transgressive surfaces and the correlatable unconformities, are real unconformity-bounded units of lower rank. Each sequence comprises transgressive, highstand and regressive systems tracts, and can be easily correlated with the depositional sequence. | Wei Jiayong (Regional Geological Survey, Xi’an College of Geology, Xi ’an 710054) Liao Nengmao Yu Youyi (Regional Geological Surveying Institute of Guizhou, Guiyang 550005) | 1996 | Journal of Earth Science1996，15，1： | 6 |
| 3 | Pathological matrix stiffness promotes cardiac fibroblast differentiation through the POU2F1 signaling pathway显示文摘Cardiac fibroblast(CF)differentiation into myofibroblasts is a crucial cause of cardiac fibrosis,which increases in the extracellular matrix(ECM)stiffness.The increased stiffness further promotes CF differentiation and fibrosis.However,the molecular mechanism is still unclear.We used bioinformatics analysis to find new candidates that regulate the genes involved in stiffnessinduced CF differentiation,and found that there were binding sites for the POU-domain transcription factor,POU2F1(also known as Oct-1),in the promoters of 50 differentially expressed genes(DEGs)in CFs on the stiffer substrate.Immunofluorescent staining and Western blotting revealed that pathological stiffness upregulated POU2F1 expression and increased CF differentiation on polyacrylamide hydrogel substrates and in mouse myocardial infarction tissue.A chromatin immunoprecipitation assay showed that POU2F1 bound to the promoters of fibrosis repressors IL1R2,CD69,and TGIF2.The expression of these fibrosis repressors was inhibited on pathological substrate stiffness.Knockdown of POU2F1 upregulated these repressors and attenuated CF differentiation on pathological substrate stiffness(35 kPa).Whereas,overexpression of POU2F1 downregulated these repressors and enhanced CF differentiation.In conclusion,pathological stiffness upregulates the transcription factor POU2F1 to promote CF differentiation by inhibiting fibrosis repressors.Our work elucidated the crosstalk between CF differentiation and the ECM and provided a potential target for cardiac fibrosis treatment. | Mingzhe Li Jimin Wu Guomin Hu Yao Song Jing Shen Junzhou Xin Zijian Li Wei Liu Erdan Dong Ming Xu Youyi Zhang Han Xiao | 2021 | Science China(Life Sciences)2021，64，2： | 3 |
| 4 | OPTIMAL RESERVE PRICE IN STATIC AND DYNAMIC SPONSORED SEARCH AUCTIONS显示文摘Sponsored search advertising is a significant revenue source for search engines.To ameliorate revenues,search engines often set fixed or variable reserve price to in influence advertisers' bidding. This paper studies the optimal reserve price for a generalized second-price auction(GSP) under both static and dynamic settings.We show that if advertisers' per-click value has an increasing generalized failure rate,the search engine's revenue rate is quasi-concave and hence there exists an optimal reserve price under both settings.Different from a static GSP auction where the optimal reserve price is proved to be constant,in a dynamic setting the optimal reserve price is dependent on not only advertisers' per-click values,but also the number of ad links sold.A search engine should gradually raise reserve price as more qualified advertisers arrive,and maintain the same threshold after all first-page positions are occupied. | Wei YANG Jun QIAO Youyi FENG Baichun XIAO | 2013 | Journal of Systems Science and Systems Engineering2013，22，4： | 2 |
| 5 | Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation显示文摘Macrophage migration inhibitory factor(MIF) is an inflammatory cytokine. It is elevated early in the blood of acute myocardial infarction patients. However, it is unclear whether and how MIF is released. This study investigated the cellular source and mechanism of MIF release from hearts. An ischemia-mimic treatment induced the secretion of MIF from neonatal rat cardiomyocytes but not from fibroblasts. The treatment did not cause significant leakage of lactate dehydrogenase, suggesting that ischemia induced the MIF secretion without causing severe cell damage. Plasma samples from patients with acute chest pain at the emergency department were collected for the detection of MIF. MIF levels in patients with acute coronary syndrome(ACS)increased early, when cardiac injury markers were not yet elevated, suggesting that ischemia can induce MIF secretion before the occurrence of severe myocardial damage. Serum-starvation caused MIF secretion from rat cardiomyocytes and Langendorffperfused rat hearts. The secretion was suppressed by the inhibition of autophagy by inhibitors or by silencing of Atg5. In conclusion, serum-starvation induces the secretion of MIF from cardiomyocytes via autophagy dependent pathway. Clarifying the mechanism of MIF secretion will be helpful for its application in the early diagnosis and treatment of ACS. | Jimin Wu Xiangning Deng Juan Gao Wei Gao Han Xiao Xinyu Wang Youyi Zhang | 2019 | Science China(Life Sciences)2019，62，8： | 2 |
| 6 | Single-molecule imaging reveals the stoichiometry change of epidermal growth factor receptor during transactivation by β_2-adrenergic receptor显示文摘Stimulation of G protein-coupled receptors(GPCRs) can lead to the transactivation of the epidermal growth factor receptors(EGFR). The cross-communication between the two signaling pathways regulates several important physiological or pathological processes. However, the molecule mechanism underlying EGFR transactivation remains poorly understood. Here, we aim to study the GPCR-mediated EGFR transactivation process using the single-molecule fluorescence imaging and tracking approach.We found that although EGFR existed as monomers at the plasma membrane of resting cells, they became dimers and thus diffused slower following the activation of β2-adrenergic receptor(β2-AR) by isoproterenol(ISO). We further proved thatβ2-AR-mediated changes of EGFR in stoichiometry and dynamics were mediated by Src kinase. Thus, the observations obtained via the single-molecule imaging and tracking methods shed new insights into the molecular mechanism of EGFR transactivation at single molecule level. | Mingliang Zhang Kangmin He Jimin Wu Nan Li Jinghe Yuan Wei Zhou Zi Ye Zijian Li Han Xiao Zhizhen Lv Youyi Zhang Xiaohong Fang | 2017 | Science China Chemistry2017，60，10： | 1 |
| 7 | Synthesis and photoconductive characteristics of biphenylporphyrin-containing poly (amic acid)s and polyimides 显示文摘 | BaoKu Zhu YouYi Xu XiuZhen Wei | 2004 | Polymer International2004，53，： | 1 |
| 8 | Permian and Triassic depositional history of the Yangtze Platform and Great Bank of Guizhou in the Nanpanjiang Basin of Guizbou and Guangxi, South China 显示文摘 | Lehrmmm D J Enos P Payne J L Montgomery P Wei Jiayong Yu Youyi Xiao Jiafei Orchard M J | 2005 | Albertiana2005，33，： | 1 |
| 9 | Permian-Triassic boundary and a Lower-Middle Tri- assic boundary sequence on the Great Bank of Guizhou, Nanpanjiang Basin, southern Guizhou Province 显示文摘 | Lehrmann D J P Payne J L E Montgomery P Wei Jiayong Yu Youyi Orchard M J | 2005 | Albertiana2005，33，： | 1 |
| 10 | Analysis of steel-reinforced concrete-filled steel tubular(SRCFST) columns under cyclic loading显示文摘 | CHANG XU WEI YOUYI YUN YANCHUN | 2012 | Construction and Building Materials2012，28，： | 1 |
| 11 | Chemiluminescence micro flow system for rapid determination of chemical oxygen demand in water显示文摘 | Liu Wei Zhang Zhujun Zhang Youyi | 2008 | Microchim Acta2008，160，： | 1 |
| 12 | Unified stress-strain model of concrete for FRP-confined columns显示文摘 | WEI YOUYI WU YUFEI | 2012 | Construction and Building Materials2012，26，1： | 1 |
| 13 | Polymorphism study of nine SNPs associated with subjective response to alcohol in Chinese Han, Hui, Tibetan, Mongolian and Uygur populations显示文摘Heavy alcohol drinking is a major public health problem,causing a large disease,social and economic burden in societies.Subjective response (SR) to alcohol is an intermediate characteristic of heavy drinking.A variety of candidate genes have been reported to be associated with SR to alcohol.In this study,we investigated nine single nucleotide polymorphisms (SNPs) related to SR to alcohol in healthy individuals from five Chinese ethnic groups,the Han,Hui,Tibetan,Mongolian and Uygur populations,and a total of 584 bloodstain samples were collected.The nine SNPs included four SNPs in alcohol-metabolizing genes (ADH1B,ADH1C,ALDH2 and CYP2E1*5B) and five SNPs in genes of neurobiological pathways (GABRA2,OPRM1,CHRNA3,HYKK and SLC6A4).A SNaPshot analysis method was developed to type these SNPs simultaneously,and all samples were typed successfully.Statistical analyses of the allele frequencies indicated that the frequencies of all SNPs,except for ADH1C,showed varying degrees of difference in the five studied ethnic groups.Tibetans showed the highest frequencies of risk alleles for heavy drinking at most loci.The genetic polymorphic differences found in this study revealed the variation in genetic susceptibility to heavy drinking in the studied populations. | Qingtao Wei Yi Ye Fan Chen Jienan Li Hao Wu Yingqiang Fu Youyi Yan Linchuan Liao | 2018 | Forensic Sciences Research2018，3，2： | 1 |
| 14 | Lower Triassic peritidal cyclic limestone: an example of anachronistic carbonate facies from the Great Bank of Guizhou, Nanpanjiang Basin, Guizhou province, South China显示文摘 | Daniel J Lehrmann Yang Wan Jiayong Wei YouYi Yu Jiafei Xiao | 2001 | Palaeogeography Palaeoclimatology Palaeoecology2001，，3： | 1 |
| 15 | Analysis of α_1-adrenoceptor subtypes in neonatal rat heart显示文摘The expression levels of 3 subtypes (α1A, α1B and α1D) of α1-adrenoceptor have been determined by radioligand binding assay and reverse-transcription polymerase chain reaction (RT-PCR) assay in neonatal rat heart. mRNAs for all the 3 subtypes have been detected in RT-PCR assays. The density of total α1-AR ( Bmax) was (134±4) fmol/mg protein and the Kd value was (164 ± 15) pmol/L. The α1-adrenoceptor subtypes selective antagonists, RS17053, ( + )niguldipine, 5-MU, BMY 7378 and WB4101, competitively inhibited binding of 125I-BE 2254 to α1-adrenoceptor in a concentration-dependent manner. The inhibition curves were better fit for the two-site model. The binding sites for α1A-, α1B- and α1D-adrenoceptor amounted to 25% , 50% and 25% of the total binding sites, respectively. | WEI Hong, L Zhizhen, ZHANG Youyi and HAN QideInstitute of Vascular Medicine , The Third Hospital, Beijing Medical University , Beijing 100083 , China | 1999 | Chinese Science Bulletin1999，44，12： | 0 |
| 16 | Exercise training attenuates angiotensinⅡ-induced cardiac fibrosis by reducing POU2F1 expression显示文摘Background:Exercise training protects against heart failure.However,the mechanism underlying the protective effect of exercise training on angiotensinⅡ(AngⅡ)-induced cardiac fibrosis remains unclear.Methods:An exercise model involving C57BL/6N mice and 6 weeks of treadmill training was used.AngⅡ(1.44 mg/kg/day)was administered to induce cardiac fibrosis.RNA sequencing and bioinformatic analysis were used to identify the key factors mediating the effects of exercise training on cardiac fibrosis.Primary adult mouse cardiac fibroblasts(CFs)were used in vitro.Adeno-associated virus serotype 9 was used to overexpress POU domain,class 2,transcription factor 1(POU2F1)in vivo.Results:Exercise training attenuated AngⅡ-induced cardiac fibrosis and reversed 39 gene expression changes.The transcription factor regulating the largest number of these genes was POU2F1.Compared to controls,POU2F1 was shown to be signififcantly upregulated by AngⅡ,which is itself reduced by exercise training.In vivo,POU2F1 overexpression nullified the benefits of exercise training on cardiac fibrosis.In CFs,POU2F1 promoted cardiac fibrosis.CCAAT enhancer-binding proteinβ(C/EBPβ)was predicted to be the transcription factor of POU2F1and verified using a dual-luciferase reporter assay.In vivo,exercise training activated AMP-activated protein kinase(AMPK)and alleviated the increase in C/EBPβinduced by AngⅡ.In CFs,AMPK agonist inhibited the increase in C/EBPβand POU2F1 induced by Ang II,whereas AMPK inhibitor reversed this effect.Conclusion:Exercise training attenuates AngⅡ-induced cardiac fibrosis by reducing POU2F1.Exercise training inhibits POU2F1 by activating AMPK,which is followed by the downregulation of C/EBPβ,the transcription factor of POU2F1. | Na Feng Haiyi Yu Yueshen Wang Youyi Zhang Han Xiao Wei Gao | 2023 | Journal of Sport and Health Science2023，12，4： | 0 |
| 17 | Determination of Chlorpyrifos in Human Blood by Gas Chromatography-Mass Spectrometry显示文摘Gas chromatography-mass spectrometry method was developed for the qualitative and quantitative analyses of chlorpyrifos in human blood samples.The chlorpyrifos and parathion(internal standard)in human blood were extracted with a mixed solvent of hexane and acetonitrile.Chlorpyrifos was well separated from the internal standard.The linear range of chlorpyrifos was 0.01-2 μg/ml in blood.The limit of detection and limit of quantification were estimated at 0.002 and 0.01μg/ml,respectively.The inter-and intra-day precisions,accuracy,and recovery were assessed to verify this method.The results showed that the developed method is rapid,sensitive,and reliable.It is suitable for the determination of chlorpyrifos in forensic toxicological analysis and clinical diagnosis. | Xinhua Dai Fei Fan Yi Ye Fan Chen Zhigui Wu Xiang Lu Qingtao Wei Jianxia Chen Youyi Yan Linchuan Liao | 2017 | Journal of Forensic Science and Medicine2017，3，1： | 0 |
| 18 | Association between serotonin transporter gene polymorphisms and non-lesional temporal lobe epilepsy in a Chinese Han population显示文摘Serotonin (5-hydroxytryptamine, 5-HT) influences the cortical and subcortical excitatory/inhibitory balance and participates in the pathophysiological processes of epilepsy. The serotonin transporter (5-HTT) is the most important factor in serotonin inactivation. We tested whether 5-HTT polymorphisms are involved in the pathogenesis of epilepsy in Chinese Han population. We did not find a significant difference in the frequencies of genotypes and alleles in the 5-HTT gene-linked polymorphic region (5-HTTLPR) in patients with non-lesional temporal lobe epilepsy and normal controls (P > 0.05). Frequencies of the 5-HTT intron 2 variable number tandem repeat (5-HTTVNTR) 12/12 genotype and allele 12 were higher in the patients with non-lesional temporal lobe epilepsy than normal controls (P < 0.01). The odds ratio of affecting non-lesional temporal lobe epilepsy was 1.435 (95% CI, 1.096-1.880) in patients carrying allele 12 (P < 0.05). Although the 5-HTTLPR may not be a genetic locus of non-lesional temporal lobe epilepsy in Chinese Han population, allele 12 in the 5-HTTVNTR may correlate with non-lesional temporal lobe epilepsy. The Stin2.12 allele and 12/12 genotype could be predisposing to non-lesional temporal lobe epilepsy. | Fengyuan Che Youyi Wei Xueyuan Heng Qingxi Fu Jianzhang Jiang | 2010 | Neural Regeneration Research2010，5，16： | 0 |
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