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| 1 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 2 | Non-ammonia enrichment of rare earth by magnesium oxide from rare earth leaching liquor in magnesium salt system显示文摘In order to solve the problem of ammonia-nitrogen pollution in the enrichment process of the ionadsorption type rare earth ore,the technology of non-ammonia precipitation with magnesium oxide precipitant was carried out.It is determined that the rare earth precipitation efficiency is 99.6%and the purity of rare earth concentrates is only 85.89 wt%under the optimum precipitation conditions.And the contents of MgO,SO3 and Al2O3 in the rare earth concentrates are 5.12 wt%,6.77 wt%and 1.78 wt%,respectively.Furthermore,the thermo-decomposition process of precipitates was investigated by TGDSC,XRD and FI-IR.The thermal decomposition process consists of two stages:the dehydration of rare earth hydroxide and alkaline rare earth sulfate within 900℃and the thermal decomposition of RE2O2SO4 at 900-1300℃.Therefore,a high-temperature calcinations method for removing SO3 from precipitates is proposed.When the precipitates are calcined at 1300℃for 2 h,the rare earth concentrates with a purity of 92.03 wt%can be acquired.Moreover,the content of SO3 in the concentrate is only 0.46 wt%.In the MgO precipitation and high-temperature calcinations process,the raw material cost is low and the quality of rare earth concentrates is acceptable.It could have great significance for nonammonia enrichment of rare earth from the rare earth leaching liquor,and finally solve the problem of ammonia nitrogen in the extraction process of the ion-adsorption type rare earth ore within magnesium salt system. | Li Huang Guohua Gao Ran Wu Qian Zhang Fuguo Lai Yanfei Xiao | 2019 | Journal of Rare Earths2019，37，8： | 14 |
| 3 | Expert consensus on the glycemic management of critically ill patients显示文摘Introduction The incidence of hyperglycemia is 40-60%in critically ill patients and is up to 60-80%in those who have undergone car-diac surgery.[1]The results of an epidemiological study in the United States showed that 28.6%of diabetic patients and 9.3%of non-diabetic patients had elevated mean daily glucose on the day of ICU admission.[2]In critically ill patients,elevated blood glucose is primarily the result of stress,and stress-induced hy-perglycemia is an independent risk factor associated with prog-nosis,regardless of a previous diagnosis of diabetes.Nutritional therapy has become an integral treatment option for patients in the ICU,[3,4]though nearly 30%of patients with enteral nu-trition and 44-50%with parenteral nutrition(PN)experience elevated glucose.[5,6]Intensive insulin therapy(IIT)is an impor-tant treatment for controlling hyperglycemia in critically ill pa-tients,but it also carries a corresponding risk of hypoglycemia. | Zhixiong Wu Jiao Liu Dong Zhang Kai Kang Xiangrong Zuo Qianghong Xu Aijun Pan Wei Fang Fen Liu You Shang Haiyan Yin Juntao Hu Jinglun Liu Jiangquan Fu Wei Zhang Yuan Zong Min Shao Feng Zhao Mei Meng Yanfei Mao Yingchuan Li Dechang Chen | 2022 | Journal of Intensive Medicine2022，2，3： | 9 |
| 4 | New sensor technologies in quality evaluation of Chinese materia medica:2010–2015显示文摘New sensor technologies play an important role in quality evaluation of Chinese materia medica(CMM) and include near-infrared spectroscopy,chemical imaging,electronic nose and electronic tongue.This review on quality evaluation of CMM and the application of the new sensors in this assessment is based on studies from 2010 to 2015,with prospects and opportunities for future research. | Xiaosu Miao Qingyu Cui Honghui Wu Yanjiang Qiao Yanfei Zheng Zhisheng Wu | 2017 | Acta Pharmaceutica Sinica B2017，7，2： | 7 |
| 5 | N-doped porous carbon nanofibers sheathed pumpkin-like Si/C composites as free-standing anodes for lithium-ion batteries显示文摘Dramatic capacity fading and poor rate performance are two main obstacles that severely hamper the widespread application of the Si anode owing to its large volume variation during cycling and low intrinsic electrical conductivity.To mitigate these issues,free-standing N-doped porous carbon nanofibers sheathed pumpkin-like Si/C composites(Si/C-ZIF-8/CNFs)are designed and synthesized by electrospinning and carbonization methods,which present greatly enhanced electrochemical properties for lithium-ion battery anodes.This particular structure alleviates the volume variation,promotes the formation of stable solid electrolyte interphase(SEI)film,and improves the electrical conductivity.As a result,the as-obtained free-standing Si/C-ZIF-8/CNFs electrode delivers a high reversible capacity of 945.5 mAh g^(-1) at 0.2 A g^(-1) with a capacity retention of 64% for 150 cycles,and exhibits a reversible capacity of 538.6 mA h g^(-1) at 0.5 A g^(-1) over 500 cycles.Moreover,the full cell composed of a freestanding Si/C-ZIF-8/CNFs anode and commercial LiNi_(1/3)Co_(1/3)Mn_(1/3)O_(2)(NCM)cathode shows a capacity of 63.4 mA h g^(-1) after 100 cycles at 0.2 C,which corresponds to a capacity retention of 60%.This rational design could provide a new path for the development of high-performance Si-based anodes. | Yanfei Zeng Yudai Huang Niantao Liu Xingchao Wang Yue Zhang Yong Guo Hong-Hui Wu Huixin Chen Xincun Tang Qiaobao Zhang | 2021 | Journal of Energy Chemistry2021，30，3： | 6 |
| 6 | An experimental study of phase transformations in olivine under pressure and temperature conditions corresponding to the mantle transition zone显示文摘High-pressure polymorphs of olivine (wadsleyite and ringwoodite) are major minerals in the mantle transition zone (MTZ).Phase transformations in olivine are important for a series of geodynamic problems such as the mineralogical and evolutionary history of the mantle,mantle convection patterns,and deep focus earthquakes in subduction zones.In this study,we examine phase transformations in olivine with two compositions,namely Mg 2 SiO 4 (Fo 100) and (Mg 0.9 Fe 0.1) 2 SiO 4 (Fo 90),at pressures between 14.1 and 20 GPa and a constant temperature of 1400°C,using the newly installed multi-anvil system at the Laboratory for Studies of the Earth's Deep Interior (SEDI),China University of Geosciences (Wuhan).At 14.1 GPa,Fo 90 transformed completely into the wadsleyite structure (β),while Fo 100 remained as olivine (α).Between 14.8 and 15.6 GPa,both Fo 100 and Fo 90 transformed into the wadsleyite structure.Wadsleyite crystals were identified by two characteristic Raman peaks between 722 and 723 and 917 and 919 cm 1.They exhibit a bimodal grain size distribution:large-crystals with average grain sizes greater than 100 μm and microcrystals less than 10 μm.The population of microcrystals increased with pressure,apparently due to the increase in over-pressure (the difference between the experimental pressure condition and the equilibrium transformation pressure at 1400°C),which promotes nucleation and retards grain growth.All run charges contained large numbers of wadsleyite microcrystals,because of the low activation energy of the nucleation process.The experimentally observed microstructure may shed light on the morphology of wadsleyite observed in shocked meteorites.At 19.5 GPa,wadsleyite coexisted with ringwoodite (γ) in Fo 100,but was absent in Fo 90.At 20 GPa,both samples transformed completely into ringwoodite,which was characterized by the 798 and 840 cm 1 Raman lines.Ringwoodite crystals are euhedral grains (average grain size 10-20 μm),with well-developed triple junctions.The complex upper mantle structure in eastern China determined from seismological studies cannot be explained by the simple transformation sequence of the olivine system alone.Phase transformations in other pyroxene-normative components (including pyroxenes and garnets) and the interaction of these components with olivine may be responsible for the complex structure.High-pressure and high-temperature experimental studies on complex systems (e.g.olivine-pyroxene),combined with data from geophysical exploration,may help in establishing a more realistic geological-petrological model for eastern China and further our understanding of the possible physical mechanisms that are responsible for the complex structure.Such studies will have profound implications for understanding the dynamic processes in the deep Earth interior. | WU Yao WANG YanBin ZHANG YanFei JIN ZhenMin WANG Chao ZHOU ChunYin | 2012 | Chinese Science Bulletin2012，57，8： | 5 |
| 7 | Epigallocatechin-3-gallate inhibits angiotensin Il-induced cardiomyocyte hypertrophy via regulating Hippo signaling pathway in H9c2 rat cardiomyocytes显示文摘AngiotensinⅡ(All) has been well known to induce cardiomyocyte hypertrophy. Epigallocatechin-3-gallate (EGCG) is the main active comp on ent of green tea and it has bee n show n to exhibit strong cardioprotective potential, although the underlying molecular mechanisms remain unclear. In this study, we investigated the role and mechanism of EGCG in preventing All-induced cardiomyocyte hypertrophy using rat H9c2 cardiomyocytes cells. Reactive oxygen species assay, cell size, and mRNA expression of cardiac hypertrophy markers ANP and BNP were assessed in response to All treatment. In addition, expression of proteins involved in Hippo signaling pathway were determined by western blot analysis. We found that All treatment resulted in significant upregulation of ANP and BNP expression levels and increase in H9c2 cell size, which were markedly attenuated by EGCG treatment. Furthermore, our results suggested that EGCG inhibited Allinduced cardiac hypertrophy via regulating the Hippo signaling pathway. Therefore, EGCG may be an effective agent for preventing cardiac hypertrophy. | Yuan Ma Yongjia Hu Jiawen Wu Junru Wen Sen Li Lijuan Zhang Jie Zhang Yanfei Jue Li | 2019 | Acta Biochimica et Biophysica Sinica2019，51，4： | 4 |
| 8 | Strengthening in Al-,Mo-or Ti-doped CoCrFeNi high entropy alloys:A parallel comparison显示文摘In the current work,a parallel comparison of the influence of Al,Mo and Ti,on the microstructure and strengthening of the CoCrFeNi alloy was conducted.To achieve this,inconsistencies on variables including the extent of alloying,thermomechanical processing and property-evaluation method were avoided.Microstructurally,following cold-rolling,annealing of the 4 at.%Al-doped alloys at 800-1000℃ did not result in phase separation;nevertheless,that of the 4 at.%Mo-and Ti-doped alloys led to the respective formation ofσandηphase and,consequently,caused extra strengthening through the Orowan dislocation bypassing mechanism.Our systematic qualitative analysis and DFT calculations showed that Al and Ti are more effective than Mo in reducing the stacking fault energy(SFE)of the CoCrFeNi alloy,because they can induce more considerable deformation of electronic density,making the gliding of atomic layers easier.Following identical thermomechnical processing,Al-,Mo-,and Ti-doping causes different extent of solid solution strengthening and grain boundary strengthening.Mo causes the most pronounced solid solution strengthening but does not benefit the grain boundary strengthening;in contrast,the effectiveness of grain boundary strengthening is boosted by the doping Al and Ti.Current analyses support that Labusch instead of Fleischer mechanism is applicable to explain the differences in solid solution strengthening,and the observed differences in grain boundary strengthening arise from the different tendency of Al,Mo and Ti to reduce the SFE of CoCrFeNi.In addition,we determined the value of the dimensionless parameter f in the Labusch model for CoCrFeNi-based alloys and observed a close relation between Hall-Petch slope and SFE.Although more in-depth studies are needed to provide full and mechanistic understandings,both these findings in fact presents significant values toward designing novel singlephase high-strength CoCrFeNi-based alloys through manipulating the solid solution and grain boundary strengthening by compositional tuning. | Xi Li Zhongtao Li Zhenggang Wu Shijun Zhao Weidong Zhang Hongbin Bei Yanfei Gao | 2021 | Journal of Materials Science & Technology2021，，35： | 4 |
| 9 | Reaction thermodynamics and kinetics on in situ Al_2O_3/Cu composites显示文摘The preferred internal oxidation of aluminum in Cu Al alloy was used to obtain in situ Al 2O 3/Cu composites. The reinforcement particles were mainly γ Al 2O 3, some θ Al 2O 3 and a little α Al 2O 3. Thermodynamics analyses show that the chemical reactions are 3Cu 2O+2Al=6Cu+Al 2O 3 or 3CuO+2Al=3Cu+Al 2O 3. A related equilibrium diagram was drawn. The experiments and investigation show that the formation rate of Al 2O 3 was controlled by the diffusion of oxygen in matrix. | Li Guobin(李国彬) 1, Wu Jianjun(武建军) 1, Guo Quanmei(郭全梅) 2, Jiang Yanfei(姜延飞) 1, Shen Yutian(申玉田) 1, Lei Tingquan(雷廷权) 2 1. Department of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, P.R.Chi | 1999 | 中国有色金属学会会刊:英文版1999，9，3： | 4 |
| 10 | Acetate-assistant efficient cation-exchange of halide perovskite nanocrystals to boost the photocatalytic CO_(2) reduction显示文摘The judicious implantation of active metal cations into the surface of semiconductor nanocrystal(NC)through cation-exchange is one of the facile and viable strategies to enhance the activity of catalysts for photocatalytic CO_(2)reduction,by shortening the transfer pathway of photogenerated carriers and increasing the active sites simultaneously.However,cation-exchange is hard to achieve for halide perovskite NCs owing to the stable octahedron of[PbX6]4−with strong interaction between halogen and lead.Herein,we report a facile method to overcome this obstacle by replacing partial Br−with acetate(Ac−)to generate CsPbBr_(3) NC(coded as CsPbBr_(3−x)Ac_(x)).A small amount of Ac−instead of Br−does not change the crystal structure of halide perovskite.Owing to the weaker interaction between acetate and lead in comparison with bromide,the corresponding octahedron structure containing acetate in CsPbBr_(3−x)Ac_(x) can be easily opened to realize efficient cation-exchange with Ni^(2+) ions.The resulting high loading amount of Ni^(2+) as active site endows CsPbBr_(3−x)Ac_(x) with an improved performance for photocatalytic CO_(2)reduction under visible light irradiation,exhibiting a significantly increased CO yield of 44.09μmol·g^(−1)·h^(−1),which is over 8 and 3 times higher than those of traditional pristine CsPbBr_(3) and nickel doped CsPbBr_(3) NC,respectively.This work provides a critical solution for the efficient metal doping of low-cost halide perovskite NCs to enhance their photocatalytic activity,promoting their practical applications in the field of photocatalysis. | Jialin Cheng Yanfei Mu Liyuan Wu Zhaolei Liu Ke Su Guangxing Dong Min Zhang Tongbu Lu | 2022 | Nano Research2022，15，3： | 3 |
| 11 | Choline-based ionic liquids for CO_2 capture and conversion显示文摘Choline-based ionic liquids(Ch-ILs) with anions possessing interacting sites to attract CO_2 were designed, which could capture CO_2 with capacity >1.0 mol CO_2 per molar IL under ambient conditions. Moreover, this kind of ILs combining with Cu Cl could catalyze the formylation of amines with CO_2/H_2 at 120 °C. Especially, choline imidazolate showed the best performance,affording a series of N-formamides in excellent yields. It was demonstrated that the IL activated CO_2 and the synergistic effect between the IL and Cu Cl resulted in the high activity for catalysing the formylation of amines with CO_2/H_2. | Ruipeng Li Yanfei Zhao Zhiyong Li Yunyan Wu Jianji Wang Zhimin Liu | 2019 | Science China Chemistry2019，62，2： | 3 |
| 12 | Research for radiation-hardened high-voltage SOI LDMOS显示文摘Based on the silicon-on-insulator(SOI) technology and radiation-hardened silicon gate(RSG) process, a radiation-hardened high-voltage lateral double-diffused MOSFET(LDMOS) device is presented in this paper. With the gate supply voltage of 30 V, the LDMOS device has a gate oxide thickness of 120 nm, and the RSG process is effective in reducing the total ionizing dose(TID) radiation-induced threshold voltage shift. The p-type ion implantation process and gate-enclosed layout topology are used to prevent radiation-induced leakage current through a parasitic path under the bird's beak and at the deep trench corner,and the device is compatible with high-voltage SOI CMOS process. In the proposed LDMOS, the total ionizing dose radiation degradation for the ON bias is more sensitive than the OFF bias. The experiment results show that the SOI LDMOS has a negative threshold voltage shift of 1.12 V, breakdown voltage of 135 V, and off-state leakage current of 0.92 pA/μm at an accumulated dose level of 100 krad(Si). | Yanfei Li Shaoli Zhu Jianwei Wu Genshen Hong Zheng Xu | 2019 | Journal of Semiconductors2019，40，5： | 3 |
| 13 | Understanding divergent domestication traits from the whole-genome sequencing of swamp-and river-buffalo populations显示文摘Domesticated buffaloes have been integral to rice-paddy agro-ecosystems formillennia,yet relatively little is known about the buffalo genomics.Here,we sequenced and assembled reference genomes for both swamp and river buffaloes and we re-sequenced 230 individuals(132 swamp buffaloes and 98 river buffaloes)sampled from across Asia and Europe.Beyond the many actionable insights that our study revealed about the domestication,basic physiology and breeding of buffalo,we made the striking discovery that the divergent domestication traits between swamp and river buffaloes can be explained with recent selections of genes on social behavior,digestion metabolism,strengths and milk production. | Xier Luo Yu Zhou Bing Zhang Yi Zhang Xiaobo Wang Tong Feng Zhipeng Li Kuiqing Cui Zhiqiang Wang Chan Luo Hui Li Yanfei Deng Fenghua Lu Jianlin Han Yongwang Miao Huaming Mao Xiaoyan Yi Cheng Ai Shigang Wu Alun Li Zhichao Wu Zijun Zhuo Do Da Giang Bikash Mitra Mohammad Farhad Vahidi Shahid Mansoor Sahar Ahmed Al-Bayatti Eka Meutia Sari Neena Amatya Gorkhali Sigit Prastowo Laiba Shafique Guoyou Ye Qian Qian Baoshan Chen Deshun Shi Jue Ruan Qingyou Liu | 2020 | National Science Review2020，7，3： | 3 |
| 14 | Cooperative effect from cation and anion of pyridine-containing anion-based ionic liquids for catalysing CO_2 transformation at ambient conditions显示文摘Pyridine-containing anion-based ionic liquids(PA-ILs) with two kinds of interaction sites to bind CO_2, e.g., [P4444][2-OP], were found to be highly efficient for catalysing the cycloaddition reactions of atmospheric CO_2 with epoxides at room temperature under metal-and halogen-free conditions, producing a series of cyclic carbonates in high yields. It was demonstrated that the cooperative interaction from two interaction sites in the anions of PA-ILs activated CO_2, while the cation activated the epoxides substrates via coordination to the central P+ unit, thus resulting in the high activity of the IL catalysts. | Guangfeng Yuan Yanfei Zhao Yunyan Wu Ruipeng Li Yu Chen Dongmei Xu Zhimin Liu | 2017 | Science China Chemistry2017，60，7： | 2 |
| 15 | Reductive Cleavage of C-O Bond in Model Compounds of Lignin显示文摘 | Huanjun Xu Xinwei Liu Yanfei Zhao Cailing Wu Yu Chen Xiang Gao Zhimin Liu | 2017 | Chinese Journal of Chemistry2017，35，6： | 2 |
| 16 | Optimal power allocation for downlink two-user non-orthogonal multiple access in visible light communication显示文摘In this work,we consider employing the NOMA(Non-Orthogonal Multiple Access)technique in downlink VLC(Visible Light Communication)for performance enhancement.In particular,focusing on a typical NOMA scenario with two users,we optimize the power allocation strategies under both sum-rate maximization and max-min fairness criteria,where practical optical power and QoS(Quality of Service)constraints are included.As our main contribution,we achieve optimal power allocation solutions in semi-closed forms via mathematical analysis,which,to the best of our knowledge,have not been reported in literature.Simulation results demonstrate that NOMA can provide remarkable performance gains over OMA(Orthogonal Multiple Access)in the context of VLC downlinks. | Hong Shen Yanfei Wu Wei Xu Chunming Zhao | 2017 | Journal of Communications and Information Networks2017，2，4： | 2 |
| 17 | EMC10 governs male fertility via maintaining sperm ion balance显示文摘Infertility is a severe public health problem worldwide that prevails up to 15% in reproductive-age couples,and male infertility accounts for half of total infertility.Studies on genetically modified animal models have identifed lots of genes involved in the pathogenesis of male infertility.The underlying causes,however,remain largely unclear.In this study,we provide evidence that EMCIO,one subunit of endoplasmic reticulum (ER)membrane protein complex (EMC),is required for male fertility.EMC10 is significantly decreased in spermatozoa from patients with asthenozoospermia and positively associated with human sperm motility. Male mice lacking Emc10 gene are completely sterile.Emc10-null spermatozoa exhibit multiple defects including abnormal morphology,decreased motility,impaired capacitation,and impotency of acrosome reaction,thereby which are incapable of fertilizing intact or ZP-free oocytes.However,intracytoplasmic sperm injection could rescue this defect caused by EMC10 deletion. Mechanistically,EMC10 deficiency leads to inactivation of Na/K-ATPase,in turn giving rise to an increased level of intraceltutar Na^+ in spermatozoa,which contributes to decreased sperm motility and abnormal morphology.Other mechanistic investigations demonstrate that the absence of EMC10 results in a reduction of HCO3^- entry and subsequent decreases of both cAMP-dependent protein kinase A substrate phosphorylation and protein tyrosine phosphorytation.These data demonstrate that EMC10 is indispensable to mate fertility via maintaining sperm ion balance of Na^+ and HCO3^-,and also suggest that EMC10 is a promising biomarker for male fertility and a potential pharmaceutical target to treat male infertility. | Yuchuan Zhou Fei Wu Man Zhang Zuquan Xiong Qianqian Yin Yanfei Ru Huijuan Shi Jinsong Li Shanhua Mao Yanliang Li Xinyi Cao Renming Hu Chong Wee Liew Qiang Ding Xuanchun Wang Yonglian Zhang | 2018 | Journal of Molecular Cell Biology2018，10，6： | 2 |
| 18 | Living cell for drug delivery显示文摘The living cells have been emerged as useful platforms for drug delivery due to their advantages of good liquidity,stability,and low immunogenicity.In this review,we summarized the development of living cells-based drug delivery systems.The drug loading methods,applications,and advantages of living cell drug delivery systems were summarized.Different living cells for drug delivery,the mechanisms of action,therapeutic applications,as well as main features were summarized and highlighted.The recent research progress and challenges were discussed.The future directions and prospects were proposed. | Yuwei Wu Yanfei Liu Ting Wang Qiao Jiang Feng Xu Zhenbao Liu | 2022 | Engineered Regeneration2022，3，2： | 1 |
| 19 | Biodiesel production from rice straw and restaurant waste employing black soldierfly assisted by microbes显示文摘 | Zheng Longyu Hou Yanfei Li Wu et a/ | 2012 | Energy2012，47，： | 1 |
| 20 | Application of Headspace Solid-Phase Microextraction and Multivariate Analysis for the Differentiation Between Edible Oils and Waste Cooking Oil显示文摘 | Dawei Tu Hong Li Zhen Wu Bo Zhao Yanfei Li | 2014 | Food Analytical Methods2014，，6： | 1 |
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