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| 1 | Suppression of m6A reader Ythdf2 promotes hematopoietic stem cell expansion显示文摘 | Zhenrui Li Pengxu Qian Wanqing Shao Hailing Shi Xi C. He Madelaine Gogol Zulin Yu Yongfu Wang Meijie Qi Yunfei Zhu John M. Perry Kai Zhang Fang Tao Kun Zhou Deqing Hu Yingli Han Chongbei Zhao Richard Alexander Hanzhang Xu Shiyuan Chen Allison Peak Kathyrn Hall Michael Peterson Anoja Perera Jeffrey S. Haug Tari Parmely Hua Li Bin Shen Julia Zeitlinger Chuan He Linheng Li | 2018 | Cell Research2018，28，9： | 18 |
| 2 | Wnt and BMP signaling crosstalk in regulating dental stem cells:Implications in dental tissue engineering显示文摘Tooth is a complex hard tissue organ and consists of multiple cell types that are regulated by important signaling pathways such as Wnt and BMP signaling.Serious injuries and/or loss of tooth or periodontal tissues may significantly impact aesthetic appearance,essential oral functions and the quality of life.Regenerative dentistry holds great promise in treating oral/dental disorders.The past decade has witnessed a rapid expansion of our understanding of the biological features of dental stem cells,along with the signaling mechanisms governing stem cell self-renewal and differentiation.In this review,we first summarize the biological characteristics of seven types of dental stem cells,including dental pulp stem cells,stem cells from apical papilla,stem cells from human exfoliated deciduous teeth,dental follicle precursor cells,periodontal ligament stem cells,alveolar bone-derived mesenchymal stem cells(MSCs),and MSCs from gingiva.We then focus on how these stem cells are regulated by bone morphogenetic protein(BMP)and/or Wnt signaling by examining the interplays between these pathways.Lastly,we analyze the current status of dental tissue engineering strategies that utilize oral/dental stem cells by harnessing the interplays between BMP and Wnt pathways.We also highlight the challenges that must be addressed before the dental stem cells may reach any clinical applications.Thus,we can expect to witness significant progresses to be made in regenerative dentistry in the coming decade. | Fugui Zhang Jinlin Song Hongmei Zhang Enyi Huang Dongzhe Song Viktor Tollemar Jing Wang Jinhua Wang Maryam Mohammed Qiang Wei Jiaming Fan Junyi Liao Yulong Zou Feng Liu Xue Hu Xiangyang Qu Liqun Chen Xinyi Yu Hue H.Luu Michael J.Lee Tong-Chuan He Ping Ji | 2016 | Genes & Diseases2016，3，4： | 16 |
| 3 | Boceprevir,GC-376,and calpain inhibitors Ⅱ,Ⅻ inhibit SARS-CoV-2 viral replication by targeting the viral main protease显示文摘A new coronavirus SARS-CoV-2,also called novel coronavirus 2019(2019-nCoV),started to circulate among humans around December 2019,and it is now widespread as a global pandemic.The disease caused by SARS-CoV-2 virus is called COVID-19,which is highly contagious and has an overall mortality rate of 6.35%as of May 26,2020.There is no vaccine or antiviral available for SARS-CoV-2.In this study,we report our discovery of inhibitors targeting the SARS-CoV-2 main protease(Mpro).Using the FRET-based enzymatic assay,several inhibitors including boceprevir,GC-376,and calpain inhibitorsⅡ,andⅫwere identified to have potent activity with single-digit to submicromolar ICs0 values in the enzymatic assay.The mechanism of action of the hits was further characterized using enzyme kinetic studies,thermal shift binding assays,and native mass spectrometry.Significantly,four compounds(boceprevir,GC-376,calpain inhibitorsⅡandⅫ)inhibit SARS-CoV-2 viral replication in cell culture with EC50 values ranging from 0.49 to 3.37μM.Notably,boceprevir,calpain inhibitorsⅡandⅫrepresent novel chemotypes that are distinct from known substrate-based peptidomimetic Mpro inhibitors.A complex crystal structure of SARS-CoV-2 Mpro with GC-376,determined at 2.15(A)resolution with three protomers per asymmetric unit,revealed two unique binding configurations,shedding light on the molecular interactions and protein conformational flexibility underlying substrate and inhibitor binding by Mpro.Overall,the compounds identified herein provide promising starting points for the further development of SARS-CoV-2 therapeutics. | Chunlong Ma Michael Dominic Sacco Brett Hurst Julia Alma Townsend Yanmei Hu Tommy Szeto Xiujun Zhang Bart Tarbet Michael Thomas Marty Yu Chen Jun Wang | 2020 | Cell Research2020，30，8： | 13 |
| 4 | 声波激励的超小型纳机电系统磁电天线显示文摘当前的小型天线依赖于电磁波的谐振,因此天线的尺寸与电磁波波长相关。典型的天线尺寸大于波长的1/10,而进一步缩小天线尺寸在近十年来都是一个公开的挑战。介绍一种通过悬浮铁氧/压电薄膜异质结构的声波激励纳机械磁电天线。这种磁电天线通过其在声波谐振频率处的磁电效应接收和发射电磁波。磁电天线中的体声波激发铁磁薄膜的磁化振荡,产生电磁波的辐射;反之,天线感应电磁波的磁场,得到压电电压输出。这种磁电天线(尺寸仅为波长的千分之一)比现有小型天线的尺寸小1到2个数量级,但性能却没有降低,在便携式无线通信系统中具有很大的应用潜力。 | Tianxiang Nan Hwaider Lin Yuan Gao Alexei Matyushov Guoliang Yu Huaihao Chen Neville Sun Shengjun Wei Zhiguang Wang Menghui Li Xinjun Wang Amine Belkessam Rongdi Guo Brian Chen James Zhou Zhenyun Qian Yu Hui Matteo Rinaldi Michael E.McConney BrANDon M.Howe Zhongqiang Hu John G.Jones Gail J.Brown Nian Xiang Sun 张建强(翻译) 陈鼎鼎(审校) | 2017 | 通信对抗2017，36，4： | 8 |
| 5 | Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions显示文摘Brain tumors are dynamic complex ecosystems with multiple cell types.To model the brain tumor microenvironment in a reproducible and scalable system,we developed a rapid three-dimensional(3D)bioprinting method to construct clinically relevant biomimetic tissue models.In recurrent glioblastoma,macrophages/microglia prominently contribute to the tumor mass.To parse the function of macrophages in 3D,we compared the growth of glioblastoma stem cells(GSCs)alone or with astrocytes and neural precursor cells in a hyaluronic acid-rich hydrogel,with or without macrophage.Bioprinted constructs integrating macrophage recapitulate patient-derived transcriptional profiles predictive of patient survival,maintenance of stemness,invasion,and drug resistance.Whole-genome CRISPR screening with bioprinted complex systems identified unique molecular dependencies in GSCs,relative to sphere culture.Multicellular bioprinted models serve as a scalable and physiologic platform to interrogate drug sensitivity,cellular crosstalk,invasion,context-specific functional dependencies,as well as immunologic interactions in a species-matched neural environment. | Min Tang Qi Xie Ryan C.Gimple Zheng Zhong Trevor Tam Jing Tian Reilly L.Kidwell Qiulian Wu Briana C.Prager Zhixin Qiu Aaron Yu Zhe Zhu Pinar Mesci Hui Jing Jacob Schimelman Pengrui Wang Derrick Lee Michael H.Lorenzini Deobrat Dixit Linjie Zhao Shruti B.hargava Tyler E.Miller Xueyi Wan Jing Tang Bingjie Sun Benjamin F.Cravatt Alysson R.Muotri Shaochen Chen Jeremy N.Rich | 2020 | Cell Research2020，30，10： | 6 |
| 6 | Post-stroke pain hypersensitivity induced by experimental thalamic hemorrhage in rats is region-specific and demonstrates limited efficacy of gabapentin显示文摘Intractable central post-stroke pain(CPSP) is one of the most common sequelae of stroke, but has been inadequately studied to date. In this study, we first determined the relationship between the lesion site and changes in mechanical or thermal pain sensitivity in a rat CPSP model with experimental thalamic hemorrhage produced by unilateral intra-thalamic collagenase IV(ITC) injection. Then, we evaluated the efficacy of gabapentin(GBP), an anticonvulsant that binds the voltage-gated Ca2+ channel α2δ and a commonly used anti-neuropathic pain medication. Histological case-by-case analysis showed that only lesions confined to the medial lemniscus and the ventroposterior lateral/medial nuclei of the thalamus and/or the posterior thalamic nucleus resulted in bilateral mechanical pain hypersensitivity. All of the animals displaying CPSP also had impaired motor coordination, while control rats with intra-thalamic saline developed no central pain or motor deficits. GBP had a dose-related anti-allodynic effect after a single administration(1, 10, or 100 mg/kg) on day 7 post-ITC, with significant effects lasting at least 5 hfor the higher doses. However, repeated treatment, once a day for two weeks, resulted in complete loss of effectiveness(drug tolerance) at 10 mg/kg, while effectiveness remained at 100 mg/kg, although the time period of efficacious analgesia was reduced. In addition, GBP did not change the basal pain sensitivity and the motor impairment caused by the ITC lesion, suggesting selective action of GBP on the somatosensory system. | Fei Yang Han Fu Yun-Fei Lu Xiao-Liang Wang Yan Yang Fan Yang Yao-Qing Yu Wei Sun Jia-Shuang Wang Michael Costigan Jun Chen | 2014 | Neuroscience Bulletin2014，30，6： | 5 |
| 7 | 基于形状导数和水平基函数的复合材料层合结构拓扑优化显示文摘首次利用水平基物质分布函数推出域内积分与边界积分泛函的形状导数,建立了复合材料刚性连续结构拓扑优化设计理论的新模型。通过将形状导数和增广的Lagrangian乘子法相结合,提出了复合材料结构拓扑优化敏度分析的新方法。设计边界的进化是通过人为掌握目标函数下降的速度来控制。水平基函数的曲面在不改变拓扑结构的前提下上下运动,从而通过边界的合并与分离改变嵌入其中的零水平基面上设计构件的拓扑结果。广泛的2D复合材料悬臂梁研究验证了本文中方法的有效性。 | 梁森 梁磊 仪垂杰 Wang Yu Michael | 2008 | 复合材料学报2008，25，3： | 4 |
| 8 | Key changes in denervated muscles and their impact on regeneration and reinnervation显示文摘The neuromuscular junction becomes progressively less receptive to regenerating axons if nerve repair is delayed for a long period of time.It is difficult to ascertain the denervated muscle's residual receptivity by time alone.Other sensitive markers that closely correlate with the extent of denervation should be found.After a denervated muscle develops a fibrillation potential,muscle fiber conduction velocity,muscle fiber diameter,muscle wet weight,and maximal isometric force all decrease; remodeling increases neuromuscular junction fragmentation and plantar area,and expression of myogenesis-related genes is initially up-regulated and then down-regulated.All these changes correlate with both the time course and degree of denervation.The nature and time course of these denervation changes in muscle are reviewed from the literature to explore their roles in assessing both the degree of detrimental changes and the potential success of a nerve repair.Fibrillation potential amplitude,muscle fiber conduction velocity,muscle fiber diameter,m RNA expression levels of myogenic regulatory factors and nicotinic acetylcholine receptor could all reflect the severity and length of denervation and the receptiveness of denervated muscle to regenerating axons,which could possibly offer an important clue for surgical choices and predict the outcomes of delayed nerve repair. | Peng Wu Aditya Chawla Robert J.Spinner Cong Yu Michael J.Yaszemski Anthony J.Windebank Huan Wang | 2014 | Neural Regeneration Research2014，9，20： | 3 |
| 9 | The P132H mutation in the main protease of Omicron SARSCoV-2 decreases thermal stability without compromising catalysis or small-molecule drug inhibition显示文摘Dear Editor,The ongoing SARS-CoV-2 pandemic continues to be a significant threat to global health.First reported in November 2021,the Omicron variant(B.1.1.529)is more transmissible and can evade immunity better than previous SARS-CoV-2 variants,fueling an unprecedented surge in cases.To produce functional proteins from its polyprotein,SARS-CoV-2 relies on the cysteine proteases Nsp3/papain-like protease(PLpro)and Nsp5/main protease(Mpro)/3C-like protease to cleave at three and more than 11 sites,respectively.1 Therefore,Mpro and PLpro inhibitors are considered to be one of the most promising SARS-CoV-2 antivirals.On December 22,2021,the Food and Drug Administration(FDA)issued an Emergency Use Authorization(EUA)for PAXLOVID,a ritonavir-boosted formulation of nirmatrelvir.Nirmatrelvir is a first-in-class orally bioavailable SARSCoV-2 Mpro inhibitor.2 Thus,the scientific community must vigilantly monitor potential mechanisms of drug resistance,especially because SARS-CoV-2 is naïve to Mpro inhibitors.Mutations have been well identified in variants to this point.3 Notably,Omicron Mpro(OMpro)harbors a single mutation—P132H.In this study,we characterized the enzymatic activity,drug inhibition,and structure of OMpro while evaluating the past and future implications of Mpro mutations. | Michael Dominic Sacco Yanmei Hu Maura Verenice Gongora Flora Meilleur Michael Trent Kemp Xiujun Zhang Jun Wang Yu Chen | 2022 | Cell Research2022，32，5： | 3 |
| 10 | Self-inflicted DNA double-strand breaks sustain tumorigenicity and stemness of cancer cells显示文摘 | Liu, Xinjian Li, Fang Huang, Qian Zhang, Zhengxiang Zhou, Ling Deng, Yu Zhou, Min Fleenor, Donald E. Wang, He Kastan, Michael B. Li, Chuan-Yuan | 2017 | Cell Research2017，27，6： | 3 |
| 11 | Level set band method: A combination of density-based and level set methods for the topology optimization of continuums显示文摘The level set method(LSM),which is transplanted from the computer graphics field,has been successfully introduced into the structural topology optimization field for about two decades,but it still has not been widely applied to practical engineering problems as density-based methods do.One of the reasons is that it acts as a boundary evolution algorithm,which is not as flexible as density-based methods at controlling topology changes.In this study,a level set band method is proposed to overcome this drawback in handling topology changes in the level set framework.This scheme is proposed to improve the continuity of objective and constraint functions by incorporating one parameter,namely,level set band,to seamlessly combine LSM and density-based method to utilize their advantages.The proposed method demonstrates a flexible topology change by applying a certain size of the level set band and can converge to a clear boundary representation methodology.The method is easy to implement for improving existing LSMs and does not require the introduction of penalization or filtering factors that are prone to numerical issues.Several 2D and 3D numerical examples of compliance minimization problems are studied to illustrate the effects of the proposed method. | Peng WEI Wenwen WANG Yang YANG Michael Yu WANG | 2020 | Frontiers of Mechanical Engineering2020，15，3： | 3 |
| 12 | A particle damper for vibration and noise reduction显示文摘 | Zhiwei Xu Michael Yu Wang Tianning Chen | 2003 | Journal of Sound and Vibration2003，，4： | 2 |
| 13 | 各向异性结构动态特性拓扑优化设计的敏度分析显示文摘利用水平集函数建立了各向异性连续结构动态特性拓扑优化设计的理论模型,使用一种全新方法证明了物质导数公式,得到了域积分和边界积分泛函的物质导数表达式.通过将物质导数法和不等式约束增广Lagrangian乘子法相结合推出了各向异性材料结构动态特性拓扑优化设计敏度分析的公式.设计边界的进化是通过人为的使目标函数下降的速度来控制,高维水平集曲面在不改变其拓扑结构的前提下上下运动,从而使嵌入在零水平集的结构形状通过其边界的合并与断裂在设计域内自动地改变其拓扑结构.广泛的2D正交各向异性悬臂梁研究验证了该方法的有效性,其结论为进一步发展复合材料结构的拓扑优化设计理论与算法奠定了基础. | 梁森 仪垂杰 郭健翔 Michael Yu Wang | 2008 | 青岛理工大学学报2008，29，3： | 2 |
| 14 | Comparison of pencil beam and Monte Carlo calculations with ion chamber array measurements for patient-specific quality assurance显示文摘Objective:To determine under what conditions and criteria comparisons between calculations made with the current clinical treatment planning system(Syngo)and an in-house built TPS(TIMPS)would allow skipping of in-beam portal-specific measurements.Methods:Measurements were made with an array of 24 ion chambers in a water phantom for 227 proton and 313 carbon ion portals with and without a range shifter(RS).These measurements were compared with calculations performed with Syngo and TIMPS using metrics of average dose difference and Gamma index.Results:For proton portals without RS,if a Gamma comparison between TIMPS and Syngo passed using criteria of 90%of tested points being within 3%and 3 mm,then 74%of measurements would agree with both TIMPS and Syngo.For proton portals with RS,more than 80%of measurements would agree with both calculations using the same criteria.For carbon ion portals without RS,if a Gamma evaluation between TIMPS and Syngo passed with criteria of 90%of tested points being within 2%and 2 mm,85%of measurements would agree with both cal-culations.For carbon ion portals with RS,if a Gamma evaluation between TIMPS and Syngo passed with criteria of 90%of tested points being within 3%and 3 mm,60%of measurements would agree with both calculations.Conclusions:Both the pencil beam algorithm in Syngo and the FDC algorithm in TIMPS can provide accurate dose calculations in water for most clinical portals.For about 75%of portals,physicists can perform comparisons of calculations instead of phantom measurements to verify Syngo calculations thereby saving a large amount of beam time.There are some portals,however,such as for low-energy protons without RS and high-energy carbon ions,where agreement between the two calculations and measurements are not yet satisfactory to allow the elimination of all measurements. | Yu Deng Zhi Chen Qianxia Wang Pablo Yepes Zhuangming Shen Hongliang Chen Jie Li Michael F.Moyers | 2022 | Radiation Medicine and Protection2022，3，3： | 2 |
| 15 | HMGB1 in health and disease显示文摘 | Rui Kang Ruochan Chen Qiuhong Zhang Wen Hou Sha Wu Lizhi Cao Jin Huang Yan Yu Xue-gong Fan Zhengwen Yan Xiaofang Sun Haichao Wang Qingde Wang Allan Tsung Timothy R. Billiar Herbert J. Zeh Michael T. Lotze Daolin Tang | 2014 | Molecular Aspects of Medicine2014，，： | 2 |
| 16 | Cell-Type-Based Analysis of MicroRNA Profiles in the Mouse Brain显示文摘 | Miao He Yu Liu Xiaowo Wang Michael Q. Zhang Gregory J. Hannon Z. Josh Huang | 2012 | Neuron2012，，1： | 2 |
| 17 | An NgAgo tool for genome editing:did CRISPR/Cas9 just find a competitor?显示文摘While CRISPR/Cas9-mediated genome editing technology has been experiencing a rapid transformation during the past few years,a recent report on NgAgo-mediated singlestranded DNA-guided genome editing may offer an attractive alternative for genome manipulation.While it’s too early to predict whether NgAgo will be able to compete with or be superior to CRISPR/Cas9,the scientific community is anxiously waiting for further optimization and broader applications of the NgAgo genome editing technology. | Qiang Wei Junyi Liao Xinyi Yu Eric J.Wang Claire Wang Hue H.Luu Rex C.Haydon Michael J.Lee Tong-Chuan He | 2016 | Genes & Diseases2016，3，3： | 2 |
| 18 | Effect of closure of live poultry markets on poultry-to-person transmission of avian influenza A H7N9 virus: an ecological study显示文摘 | Hongjie Yu Joseph T Wu Benjamin J Cowling Qiaohong Liao Vicky J Fang Sheng Zhou Peng Wu Hang Zhou Eric H Y Lau Danhuai Guo Michael Y Ni Zhibin Peng Luzhao Feng Hui Jiang Huiming Luo Qun Li Zijian Feng Yu Wang Weizhong Yang Gabriel M Leung | 2013 | The Lancet2013，，： | 2 |
| 19 | A level set method for structural topology optimization显示文摘 | Michael Yu Wang Xiaoming Wang Dongming Guo | 2002 | Computer Methods in Applied Mechanics and Engineering2002，，1： | 2 |
| 20 | Engineering feature design for level set based structural optimization 显示文摘 | Mingdong Zhou Michael Yu Wang | 2013 | Computer-Aided Design2013，45，12： | 1 |
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