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| 1 | Houshiheisan compound prescription protects neurovascular units after cerebral ischemia显示文摘Houshiheisan is composed of wind-dispelling(chrysanthemun flower, divaricate saposhnikovia root, Manchurian wild ginger, cassia twig, Szechwan lovage rhizome, and platycodon root) and deficiency-nourishing(ginseng, Chinese angelica, large-head atractylodes rhizome, Indian bread,and zingiber) drugs. In this study, we assumed these drugs have protective effects against cerebral ischemia, on neurovascular units. Houshiheisan was intragastrically administered in a rat model of focal cerebral ischemia. Hematoxylin-eosin staining, transmission electron microscopy, immunofluorescence staining, and western blot assays showed that Houshiheisan reduced pathological injury to the ischemic penumbra, protected neurovascular units, visibly up-regulated neuronal nuclear antigen expression, and down-regulated amyloid precursor protein and amyloid-β 42expression. Wind-dispelling and deficiency-nourishing drugs maintained NeuN expression to varying degrees, but did not affect amyloid precursor protein or amyloid-β 42 expression in the ischemic penumbra. Our results suggest that the compound prescription Houshiheisan effectively suppresses abnormal amyloid precursor protein accumulation, reduces amyloid substance deposition, maintains stabilization of the internal environment of neurovascular units, and minimizes injury to neurovascular units in the ischemic penumbra. | Haizheng Wang Lei Wang Nan Zhang Qi Zhang Hui Zhao Qiuxia Zhang | 2014 | Neural Regeneration Research2014，9，7： | 7 |
| 2 | Bushen Yisui Capsule ameliorates axonal injury in experimental autoimmune encephalomyelitis显示文摘A preliminary clinical study by our group demonstrated Bushen Yisui Capsule(formerly called Erhuang Formula)in combination with conventional therapy is an effective prescription for the treatment of multiple sclerosis.However,its effect on axonal injury during early multiple sclerosis remains unclear.In this study,a MOG35–55-immunized C57BL/6 mouse model of experimental autoimmune encephalomyelitis was intragastrically administered Bushen Yisui Capsule.The results showed that Bushen Yisui Capsule effectively improved clinical symptoms and neurological function of experimental autoimmune encephalomyelitis.In addition,amyloid precursor protein expression was down-regulated and microtubule-associated protein 2 was up-regulated.Experimental findings indicate that the disease-preventive mechanism of Bushen Yisui Capsule in experimental autoimmune encephalomyelitis was mediated by amelioration of axonal damage and promotion of regeneration.But the effects of the high-dose Bushen Yisui Capsule group was not better than that of the medium-dose and low-dose Bushen Yisui Capsule group in preventing neurological dysfunction. | Ling Fang Qi Zheng Tao Yang Hui Zhao Qiuxia Zhang Kangning Li Li Zhou Haiyang Gong Yongping Fan Lei Wang | 2013 | Neural Regeneration Research2013，8，35： | 6 |
| 3 | Assessment on alien herbicide-resistant gene flow among crucifers by sexual compatibility显示文摘Cross-pollination and high cross-compatibility are frequently found in many cruciferous plants, which im-plies that there might be potential risk of alien gene flow among crucifers. In this work, the alien glyphosate-resistant gene flow between GM (Genetically modified) rapeseed vari-ety Q3 (Brassica napus) and 6 varieties from 5 cruciferous species was assessed by sexual compatibility through aniline blue fluorescence observation, manual cross, mentor pollina-tion with the mixture pollen and DNA identification. The results indicated that the compatibility indices of two B. napus varieties, B. juncea var. multiceps Tsen et Lee and Chinese cabbage (B. chinensis) (as female) with Q3 (as male) were 16.15, 12.77, 2.345 and 0.85, respectively. F1 plants were examined by PCR and all of these samples were confirmed as positive. Crossing between Orychophragmus violaceus, Des-curainia sophia (as female) and Q3 (as male) expressed high cross-incompatibility. Fluorescence microscope observation indicated that growing of pollen tube terminated at the upper 1/3 part of the style of O. violaceus and on the stigma surface of D. sophia. The mentor plants were further analyzed by PCR and all were shown to be negative. Under the neighboring growth and natural pollination conditions, the rates of gene flow to two B. napus varieties were 2.3278% and 2.1487%, respectively, B. juncea var. multiceps Tsen et Lee and Chinese cabbage were 1.0157% and 0.9236%, re-spectively. No gene flow was observed in O. violaceus and D. sophia. Frequency of gene flow was highly correlated with sexual compatibility. Due to possible alien gene flow among crucifers, special consideration and care should be taken to grow GM rapeseed. | ZHAO Xiangxiang LU Weiping QI Cunkou PU Huiming XIA Qiuxia LU Dalei LIU Geshan WANG Youping | 2005 | Chinese Science Bulletin2005，50，15： | 6 |
| 4 | Effects of wind-dispelling drugs and deficiency-nourishing drugs of Houshiheisan compound prescription on astrocyte activation and inflammatory factor expression in the corpus striatum of cerebral ischemia rats显示文摘This study explored protective effects of Houshiheisan and its compound prescription of wind-dispelling drugs and deficiency-nourishing drugs on cerebral ischemia in terms of astrocyte activation and inflammatory factor expression.Results suggested that Houshiheisan lessened neuronal degeneration in the corpus striatum on the ischemic side of rats following cerebral ischemia/reperfusion injury,contributed to astrocyte activation and glial fibrillary acidic protein expression in the corpus striatum and decreased the levels of interleukin-2,interleukin-6, interleukin-1βand tumor necrosis factor-α.Factor analysis results demonstrated that deficiency-nourishing drugs were more beneficial in protecting neurons and upregulating glial fibrillary acidic protein expression than wind-dispelling drugs.However,wind-dispelling drugs were more effective in increasing the number of glial fibrillary acidic protein-positive cells and reducing inflammatory factor expression than deficiency-nourishing drugs.These indicate that different ingredients of Houshiheisan suppress cerebral ischemic injury by promoting astrocyte activation and diminishing inflammatory factor expression. | Qiuxia Zhang Hui Zhao Lei Wang Qi Zhang Haizheng Wang | 2012 | Neural Regeneration Research2012，7，24： | 6 |
| 5 | Hippocampal expression of synaptic structural proteins and phosphorylated cAMP response element-binding protein in a rat model of vascular dementia induced by chronic cerebral hypoperfusion显示文摘The present study established a rat model of vascular dementia induced by chronic cerebral hypoperfusion through permanent ligation of bilateral common carotid arteries.At 60 days after modeling,escape latency and swimming path length during hidden-platform acquisition training in Morris water maze significantly increased in the model group.In addition,the number of accurate crossings over the original platform significantly decreased,hippocampal CA1 synaptophysin and growth-associated protein 43 expression significantly decreased,cAMP response element-binding protein expression remained unchanged,and phosphorylated cAMP response element-binding protein expression significantly decreased.Results suggested that abnormal expression of hippocampal synaptic structural protein and cAMP response element-binding protein phosphorylation played a role in cognitive impairment following chronic cerebral hypoperfusion. | Hui Zhao Zhiyong Li Yali Wang Qiuxia Zhang | 2012 | Neural Regeneration Research2012，7，11： | 4 |
| 6 | Embryoid bodies formation and differentiation from mouse embryonic stem cells in collagen/Matrigel scaffolds显示文摘Embryonic stem (ES) cells have the potential to develop into any type of tissue and are considered as a promising source of seeding cells for tissue engineering and transplantation therapy.The main catalyst for ES cells differentiation is the growth into embryoid bodies (EBs),which are utilized widely as the trigger of in vitro differentiation.In this study,a novel method for generating EBs from mouse ES cells through culture in collagen/Matrigel scaffolds was successfully established.When single ES cells were seeded in three dimensional collagen/Matrigel scaffolds,they grew into aggregates gradually and formed simple EBs with circular structures.After 7 days' culture,they formed into cystic EBs that would eventually differentiate into the three embryonic germ layers.Evaluation of the EBs in terms of morphology and potential to differentiate indicated that they were typical in structure and could generate various cell types;they were also able to form into tissue-like structures.Moreover,with introduction of ascorbic acid,ES cells differentiated into cardiomyocytes efficiently and started contracting synchronously at day 19.The results demonstrated that collagen/Matrigel scaffolds supported EBs formation and their subsequent differentiation in a single three dimensional environment. | Jin Zhou Ye Zhang Qiuxia Lin Zhiqiang Liu Haibin Wang Cuimi Duan Yanmeng Wang Tong Hao Kmwu Wu Changyong Wang | 2010 | Journal of Genetics and Genomics2010，37，7： | 4 |
| 7 | Cellulose Nanofibrous Membranes Modified with Phenyl Glycidyl Ether for Efficient Adsorption of Bovine Serum Albumin显示文摘Hydrophobic interaction chromatography(HIC)as an indispensable method for protein purification has attracted considerable attentions of researchers as well as biopharmaceutical industries.However,the low binding capacity and slow adsorption rate of the currently available HIC media lead to a little supply and high price of the highly purified proteins.Herein,nanofibrous membranes with hydrophobic binding sites were developed for HIC by directly coupling phenyl glycidyl ether on the hydrolyzed cellulose acetate nanofiber membrane(cellulose-phenyl NFM).Scanning electron microscope(SEM),water contact angle(WCA),Fourier transform infrared(FTIR),thermogravimetric analysis(TGA),Brunauer-Emmett-Teller(BET)surface area analysis and capillary flow porometer(CFP)were applied to evaluate the physically and chemically structural transformation.The obtained cellulose-phenyl NFMs showed a proper hydrophilcity(WCA=37°),a relatively high BET surface area(3.6 times the surface area of commercial fibrous membranes),and tortuous-channel structure with through-hole size in the range of 0.25-1.2μm,which led to a little non-specificity adsorption,high bovine serum albumin adsorption capacity of 118 mg g^(−1),fast adsorption process within 12 h,good long-term stability and reusability.Moreover,compared with traditional modification methods which always include activation and graft two steps,direct coupling method is more efficient for HIC media fabrication.Therefore,cellulose-phenyl NFMs with outstanding protein adsorption performance could be a kind of promising candidate for HIC. | Lihuan Wang Qiuxia Fu Jianyong Yu Lifang Liu Bin Ding | 2019 | Advanced Fiber Materials2019，1，3： | 3 |
| 8 | Amyloid precursor protein and growth-associated protein 43 expression in brain white matter and spinal cord tissues in a rat model of experimental autoimmune encephalomyelitis显示文摘Studies have demonstrated that amyloid precursor protein(APP)expression increases in multiple sclerosis tissues during acutely and chronically active stages.To determine the relationship between axonal injury and regeneration in multiple sclerosis,an animal model of experimental autoimmune encephalomyelitis was induced using different doses of myelin basic protein peptide.APP and growth-associated protein 43(GAP-43),which is considered a specific marker of neural regeneration,were assessed by western blot analysis.Expression of APP and GAP-43,as well as the correlation between these two proteins,in brain white matter and spinal cord tissues of experimental autoimmune encephalomyelitis rats at different pathological stages was analyzed.Results showed that APP and GAP-43 expression increased during the acute stage and decreased during remission,with a positive correlation between APP and GAP-43 expression in brain white matter and spinal cord tissues.These results suggest that APP and GAP-43 could provide nutritional and protective effects on damaged neurons. | Yizhou Wang Shuang Kou Jingcheng Tang Ping Zhang Qiuxia Zhang Yan Liu Qi Zheng Hui Zhao Lei Wang | 2011 | Neural Regeneration Research2011，6，2： | 3 |
| 9 | Control Effect of Several Replacement Plants on Invasive Weed Eupatorium adenophorum Spreng显示文摘To select the suitable plant species controlling Eupatorium adenophorum in hilly area,ecological adaptability,competitiveness and control efficacy of many replacement plants with economic value in Guizhou Province of China were studied.The results showed that the coverage of Paspalum wetsfeteini and Dolichos lablab were 100% and 93%,which increased by 3.70 and 3.44 times compared with Lolium perenne,respectively;their relative crowding coefficient with E.adenophorum were 7.09 and 22.78,which increased by 2.43 and 7.80 times compared with L perenne,respectively.Using excavation method,the control efficacies of replacement plants were 99.3 % and 96.9%,respectively,while the control efficacy of replacement plants using mowing method was lower than 66.4%.The overwintering rate of P.wetsfeteini in the following year was 95% ;its coverage was still 100% and its control efficacy against E.adenophorum remained over 99%.D.lablab was difficult to survive the winter in the north region beyond 26.2°N,so it could only be applied as annual replacement plant.The coverage of Setaria anceps,Cajanus cajan and other test plants were less than 90%,with poor control efficacy against E.adenophorum. | Lu Xiangyang Wang Qiuxia Zhang Jinhua Zuo Xiangbing Liu Bin | 2013 | Plant Diseases and Pests2013，4，2： | 3 |
| 10 | Isolation and Neuroprotective Activity of Phenolic Derivatives from the Marine-Derived Fungus Penicillium janthinellum显示文摘A new phenolic compound, 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-3-methyl-2H-pyran-2-one(1), along with other six known phenolic derivatives(2-7), were isolated from the mangrove rhizosphere fungus Penicillium janthinellum HK1-6 cultured in potato dextrose broth medium containing 30 g L^(-1) of natural sea salt. The structure of the new compound(1) was elucidated by comprehensive analysis of spectroscopic data including 1D and 2D NMR spectra. The proposed biosynthetic pathway of compound 1 was also studied in this research. Interestingly, a brominated phenolic derivative, aryl bromide(compound 8), was obtained from this fungal strain cultured in medium containing 30 g L^-1 of NaBr instead of natural sea salt. Compound 8 is proposed as a new natural product and formed through bromination of compound 7 when the fungus was cultured with NaBr. The neuroprotective effect of compound 1 on oxygen-glucose deprivation(OGD)-induced injury was investigated in rat spinal cord astrocytes. MTT assay demonstrated that compound 1 can attenuate OGD-induced cell viability loss in rat spinal cord astrocytes. | ZHENG Yaoyao CHEN Xu CHEN Lumin SHEN Li FU Xiumei CHEN Qiuxia CHEN Min WANG Changyun | 2020 | Journal of Ocean University of China2020，19，3： | 2 |
| 11 | A comparative study of proliferation and osteogenic differentiation of rat adipose-derived stem cells in β-tricalcium phosphate (β-TCP), forsterite (Mg_2SiO_4 ) and clinoenstatite (MgSiO_3)显示文摘In this study, the effects of forsterite and clinoenstatite powder extracts on the proliferation and osteogenic differentiation of rat adipose-derived stem cells (ASCs) were investigated and compared with the β-tricalcium phosphate (β-TCP) powder extracts. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and live-dead staining were performed to evaluate the viability and proliferation of rat ASCs. Osteogenic differentiation of rat ASCs were assayed by alkaline phosphatase (ALP) staining and ALP activity test. The expression of osteogenic marker genes (alkaline phosphatase (ALP), runt related transcription factor 2 (Runx2), collagen type Iα1 (Col1α1), secreted phosphoprotein1 (Spp1, osteopontin), integrin binding sialoprotein (Ibsp), bone gla protein (Bglap)) were detected by real-time polymerase chain reaction (PCR). The MTT assay and the live-dead staining showed that all the three ceramics possessed good cytocompatibility with rat ASCs. Furthermore, forsterite and clinoenstatite promoted the proliferation of rat ASCs compared with β-TCP. The results of the ALP activity test and the real-time PCR demonstrated that forsterite and clinoenstatite promoted the osteogenic differentiation of rat ASCs. These results suggested that forsterite and clinoenstatite are bioactive ceramics that may be used for preparation of bone tissue engineering (BTE) scaffolds. | LENG Bin JIN XiaoGang LIN QiuXia CHEN Lei WANG Yan DU ZhiYan LIN KaiLi CHANG Jiang GU XiaoMing WANG ChangYong | 2013 | Chinese Science Bulletin2013，58，24： | 2 |
| 12 | Comparative analysis of membranous and other nephropathy subtypes and establishment of a diagnostic model显示文摘This study aimed to compare clinical features between membranous nephropathy (MN) and nonmembranous nephropathy (non-MN), to explore the clinically difTerential diagnosis of these two types, and to establish a diagnostic model of MN. After renal biopsy was obtained, 798 patients were divided into two groups based on their examination results: primary MN group (n = 248) and non-MN group (n = 550). Their data were statistically analyzed. Logistic regression analysis indicated that anti-PLA2R antibodies, IgG, and Cr were independently correlated with MN, and these three parameters were then used to establish the MN diagnostic model. A receiver operating characteristic (ROC) curve confirmed that our diagnostic model could distinguish between patients with and without MN, and their corresponding sensitivity, specificity, and AUC were 79.9%, 89.4%, and 0.917, respectively. The cutoff value for this combination in MN diagnosis was 0.34. The established diagnostic model that combined multiple factors shows a potential for broad clinical applications in differentiating primary MN from other kidney diseases and provides reliable evidence supporting the feasibility of noninvasive diagnosis of kidney diseases. | Hanyu Zhu Bo Fu Yong Wang Jing Gao Qiuxia Han Wenjia Geng Xiaoli Yang Guangyan Cai Xiangmei Chen Dong Zhang | 2019 | Frontiers of Medicine2019，13，5： | 2 |
| 13 | Association of a potential functional pre‐miR‐218 polymorphism and its interaction with hepatitis B virus mutations with hepatocellular carcinoma risk显示文摘 | Yifang Han Rui Pu Xue Han Jun Zhao Weiping Li Jianhua Yin Yuwei Zhang Qiuxia Shen Jiaxin Xie Qi Zhang Shuang Jiang Juhong Li Hongwei Zhang Hongyang Wang Guangwen Cao | 2014 | Liver Int2014，，5： | 1 |
| 14 | Analysis of the Role of General Practitioners Services in Rural Areas during the COVID-19 Epidemic显示文摘Since the outbreak of the COVID-19,various regions of China have been rapidly deployed under the leadership of the Central Committee of the Party to actively prevent and control the COVID-19.The rural areas of my country have weak links to the prevention and management of public health emergencies.Problems include lack of medical and health resources and farmers’low awareness of epidemic prevention.Situations that correspond to the prevention and management of the COVID-19 are more serious.As the patient’s first contact and“gatekeeper”in the fight against the epidemic,the general practitioner is responsible for the“first visit-subsequent ongoing intervention”.This article is about the prevention and control of the COVID-19 epidemics and epidemic prevention in terms of dissemination of knowledge,informed crowd control,joint prevention and control,and standardized management of people.This is a summary of the efforts of general practitioners.Quarantine at home,interactive referrals to medical consortiums,special care for contracted families.The function during the management period aims to analyze the role played by general practitioners during the epidemic and to provide new ideas for the prevention and management of the epidemic.Provide more targeted general practitioner-style services in rural areas to promote the implementation and improvement of health and poverty alleviation.The health level of the rural population provides a theoretical standard. | Zuohua Liu Feiya Li Zihan Liu Qiuxia Wu Xiangyu Li Qiang Wang | 2021 | Journal of Advances in Medicine Science2021，4，2： | 1 |
| 15 | Studies on Isosorbide-enhanced Biodegradable Poly(ethylene succinate)显示文摘To improve the mechanical properties of bio-based poly(ethylene succinate),the sugar monomer isosorbide,whicli is relatively easy to obtain,was used as a copolymerized third monomer to synthesize poly(ethyleneco-isosorbide succinate),a 100%biomass copolyester.The effects of isosorbide on the crystallinity and thermal properties of copolyester were studied by nuclear magnetic resonancefH NMR),differential scanning calorimeter(DSC),and thermogravimetric(TG).Owing to its distinct rigid bicyclic structure,isosorbide can improve the glass transition temperature of the copolyester and decrease the crystallization rate,as well as accelerate the hydrolysis of the copolyester.Simultaneously,the introduction of isosorbide can effectively improve the antistatic properties of copolyester. | QU Dezhi ZHANG Fuchen GAO Hongwei WANG Qiuxia BAI Yongping LIU Huihui | 2019 | Chemical Research in Chinese Universities2019，35，2： | 1 |
| 16 | Research progress ofecological restoration for Wetlands in coal mine areas显示文摘 | Qiuxia Zhang Fang Wang Rusong Wang | 2011 | ProcediaEnvironmental Sciences2011，，10： | 1 |
| 17 | Bioluminescence imaging of caspase-3 activity in mouse liver显示文摘 | Qiuxia Fu Xiangguo Duan Shaoduo Yan Licui Wang Yong Zhou Shuaizheng Jia Juan Du Xiaohui Wang Yuhua Zhang Linsheng Zhan | 2013 | Apoptosis2013，，8： | 1 |
| 18 | A chitosan–glutathione based injectable hydrogel for suppression of oxidative stress damage in cardiomyocytes显示文摘 | Junjie Li Yao Shu Tong Hao Yan Wang Yufeng Qian Cuimi Duan Hongyu Sun Qiuxia Lin Changyong Wang | 2013 | Biomaterials2013，，36： | 1 |
| 19 | Dynamic self-assembly synthesis and controlled release as drug vehicles of porous hollow silica nanoparticles显示文摘 | Shun Tan Qiuxia Wu Jia Wang Yanli Wang Xiaolei Liu Keke Sui Xiaoyong Deng Haifang Wang Minghong Wu | 2011 | Microporous and Mesoporous Materials2011，，2： | 1 |
| 20 | The influence of chitosan hydrogel on stem cell engraftment, survival and homing in the ischemic myocardial microenvironment显示文摘 | Zhiqiang Liu Haibin Wang Yan Wang Qiuxia Lin Anning Yao Feng Cao Dexue Li Jin Zhou Cuimi Duan Zhiyan Du Yanmeng Wang Changyong Wang | 2011 | Biomaterials2011，，11： | 1 |
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