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| 1 | Qingnaoyizhi decoction suppresses the formation of glial fibrillary acidic protein-positive cells in cultured neural stem cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway显示文摘OBJECTIVE: Inactivation of the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signaling axis plays a crucial role in determining the fate of neural stem cells(NSCs).Qingnaoyizhi decoction(QNYZD) has been used for the treatment of vascular dementia and has shown to improve synaptic remodeling. The aim of this study was to evaluate the effect of cerebrospinal fluid(CSF) containing QNYZD(CSF-QNYZD) on the differentiation of cultured NSCs and the involvement of the JAK2/STAT3 pathway.METHODS: The protein expression levels of glial fibrillary acidic protein(GFAP), tubulin, drosophila mothers against decapentaplegic protein(SMAD-1), STAT3, and phosphorylated-STAT3 were detected by western immunoblot analysis in the groups: control, CSF, JAK/STAT inhibitor(AG490),CSF-QNYZD, and CSF-XDZ(CSF-Xidezhen). The differentiation of NSCs was determined by immunofluorescence staining. The proliferation of NSCs was measured using the Cell Counting Kit-8 proliferation assay.RESULTS: Compared with the control group,CSF-QNYZD and AG490 significantly increased the number and expression of tubulin-positive cells, reduced the number and expression of GFAP-positive cells, and down-regulated the expression of p-STAT3. However, CSF-QNYZD also decreased the expression of SMAD-1 and STAT3.CONCLUSION: Enhanced neuronal differentiation may be associated with the down-regulation of glial differentiation instead of promoting proliferationin treated NSCs. Furthermore, QNYZD may play a direct role in suppressing the formation of GFAP-positive cells and enhancing neuronal differentiation by inhibiting JAK2/STAT3 activation. Overall, these results provide insights into the possible mechanism underlying QNYZD-mediated neurogenesis. | Wu Yanqing Jing Zhiwei Qin Xiude Zhou Zhen Wang Kai Song Wanshan Wang Xueyan Hou Mengmeng Zhang Yulian Kang Liyuan | 2015 | Journal of Traditional Chinese Medicine2015，35，1： | 11 |
| 2 | Axonal autophagy during regeneration of the rat sciatic nerve显示文摘BACKGROUND: The removal of degenerated axonal debris during Wallerian degeneration is very important for nerve regeneration. However, the mechanism by which debris is removed is not been completely understood. Considerable controversy remains as to the clearance pathway and cells that are involved. OBJECTIVE: To investigate axonal autophagy during removal of degenerated axonal debris by transecting the sciatic nerve in a rat Wallerian degeneration model. DESIGN, TIME AND SETTING: Experimental neuropathological analysis. The experiment was conducted at the Laboratory Animal Service Center of the Southern Medical University between January and June 2005. MATERIALS: Fifty-four adult, Wistar rats of either sex, weighing 180-250 g, were obtained from the Laboratory Animal Service Center of the Southern Medical University. Animals were randomly divided into nine groups of six rats. METHODS: Wallerian degeneration was induced by transecting the rat sciatic nerve, and tissue samples from the distal stump were obtained 0.2, 0.4, 1, 2, 3, 4, 7, 10, and 15 days post-transection. Ultrathin sections were prepared for electron microscopy to study ultrastructure and enzyme cytochemistry staining. MAIN OUTCOME MEASURES: Ultrastructure (axon body, autophagic body, and cystoskeleton) of axons and myelin sheaths observed with electron microscopy; acidic phosphatase activity detected by Gomori staining using electron microscopy. RESULTS: The major changes of degenerating axons after transection were axoplasm swelling and separation of axons from their myelin sheath between five hours and two days post-transection. At four days post-transection, the axoplasm condensed and axons were completely separated from the myelin sheath, forming dissociative axon bodies. Vacuoles of different sizes formed in axons during the early phase after lesion. Larger dissociative axon bodies were formed when the axons were completely separated from the myelin sheath during a late phase. The axolemma surrounding the axon body was derived from the neuronal cell membrane; the condensed axoplasm contained many autophagic vacuoles at all levels. A large number of neurofilaments, microtubules, and microfilaments were arranged in a criss-cross pattern. The autophagic vacuoles exhibited acidic phosphatase activity. Axonal bodies were absorbed after degradation from day 7 onwards, and macrophages were observed rarely in the formative cavity. CONCLUSION: The degenerating axons were cleared mainly by axonal autophagy and Schwann cell phagocytosis during regeneration of the rat sciatic nerve, and macrophages exhibited only an assisting function. | Kangrong Lu Zhongxian Piao Zhenxi Liu Weiwang Gu Wanshan Wang Yingjie Piao | 2008 | Neural Regeneration Research2008，3，6： | 2 |
| 3 | The Integrated System of QFD and FMEA Based on CSCW显示文摘There has been some research on the theory of QFD(Quality Function Deployment)and FMEA(Failure Modes and Effect Analysis)recently,but little work to the integrated system of QFD and FMEA development.This paper analyses the technical difficulties of realization of QFD and proposes away using computer-supported collaborative work to develop the integrated system of QFD and FMEA and how to do it.The way can make the system more efficient and more suitable to new requirements of networked manufacturing enterprise.The same time,prototype system of the integrated system is developed by comprehensively ap- plication of Java、JSP、JavaServlet,the open World Wide Web platforms of which enhances the communication function,improves the group cooperation and shorts the development time.The system is verified reasonably and reliably through the example of QFD analysis of some automobile. | JIANG Xingyu GONG Yadong WANG Guihe YU Tianbiao WANG Wanshan School of Mechanical Engineering and Automation,Northeastern University,Shenyang 110004,China | 2006 | 武汉理工大学学报2006，28，S3： | 2 |
| 4 | Post-mortem tissue proteomics reveals the pathogenesis of multi-organ injuries of COVID-19显示文摘Multi-organ injuries are a major complication of severe Coronavirus Disease 2019(COVID-19).However,its pathogenesis is barely understood.Herein;we profile host responses to fatal SARS-CoV-2 infection by performing quantitative proteomics of 19 post-mortem samples of 8 different organs or tissues,including lungs,liver,intestine,kidney;spleen,brain,heart and muscle,from 3 deceased COVID-19 victims during the first wave of the pandemic(Supplementary Fig.S1A and B,Tables SI-S3). | Yang Qiu Di Wu Wanshan Ning Jiqian Xu Ting Shu Muhan Huang Rong Chen Jiancheng Zhang Yang Han Qingyu Yang Ruiting Li Xiaobo Yang Yaxin Wang Xiaojing Zou Shangwen Pan Chaolin Huang Yu Xue You Shang Xi Zhou | 2021 | National Science Review2021，8，11： | 1 |
| 5 | Research on rotary surface topography by orthogonal turn-milling显示文摘 | Zhu Lida Li Haonan Wang Wanshan | 2013 | The International Journal of Advanced Manufacturing Technology2013，69，9101112： | 1 |
| 6 | Finite Element Simulation of Chip Formation with Single Grain显示文摘 | Wang Wanshan Su Chong Pang Zirui | 2009 | Key Engineering Material2009，416，： | 1 |
| 7 | Partner selection and optimization model in global manufacturing显示文摘 | DONG Wenhui GONG Yadong WANG Wanshan | 2005 | Journal of Northeastern University2005，26，5： | 1 |
| 8 | Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellularcarcinoma显示文摘 | Wanshan Ma Xiaohong Shi Sumei Lu Linlin Wu Yunshan Wang | 2013 | Oncology Reports2013，，6： | 1 |
| 9 | Finite element analysis of metal cutting process base on LS-DYNA显示文摘 | Hou Junming Zhu Lida Wang Wanshan | | 0，，： | 1 |
| 10 | Part- ner selection and optimization model in global manufactur- ing 显示文摘 | DONG Wenhui GONG Yadong WANG Wanshan | 2005 | Journal of Northeastern University2005，26，5： | 1 |
| 11 | Integration of fuzzy AHP and FPP with TOPSIS methodology for aero- engine health assessment 显示文摘 | Wang Jianrong Fan Kai Wang Wanshan | 2010 | Expert Systems with Applications2010，37，12： | 1 |
| 12 | Integration of fuzzy AHP and FPP with TOPSIS methodology for aero engine health assessment显示文摘 | Wang Jianrong Fan Kai Wang Wanshan | 2010 | Expert Systems with Applications2010，37，12： | 1 |
| 13 | Integra-ting analytic hierarchy process and genetic algorithm for air-craft engine maintenance scheduling problem显示文摘 | WANG Jianrong YU Tianbiao WANG Wanshan | 2010 | Advances inIntelligent and Soft Computing2010，66，： | 1 |
| 14 | Different Burial-Cooling History of Triassic Strata between the Western Weibei Uplift and the Northwestern Weihe Basin in Northwest China显示文摘Analysis of tectonothermal history of the Yanchang Formation in the western Weibei Uplift and in the northwestern Weihe Basin can reconstruct the cooling history of the southwest most remained Upper Triassic source rock of the North China Plate. Apatite fission-track(AFT) and(U-ThSm)/He(AHe) analysis were used to recover the cooling and uplift history of the Upper Triassic here. Ten sandstones from the Middle–Upper Triassic strata yield AFT ages between 179.8 ± 7.4 and 127.6 ± 8.1 Ma. AHe ages of two sandstones have the value of 37.7 ± 2.3–131.1 ± 8.1 and 45.7 ± 2.8–83.5 ± 5.2 Ma. Time-temperature modeling results showed that tectonothermal history of the Yanchang Formation was initially different in time-space relationships but then became almost identical through time followed by different cooling rate. Modeling results of the Triassic strata in the Qianyang area and the Yaojiagou area revealed three different uplift-cooling stages commencing in the Late Jurassic at ~165 Ma and in Early Cretaceous at ~110 Ma, respectively, both followed by first similar cooling histories to the Early Miocene at ~20 – 23 Ma and then different since the Late Miocene. Uplift-cooling rate since the Late Miocene at ~8 Ma were different between the Western Weibei Uplift and the Northwestern Weihe Basin. The timing, cooling-uplift rates of the Yaojiagou area, which was mainly controlled by movements related to the Liupanshan Mountains, the Qinling Orogens and the Weibei Uplift, had the earliest onset of uplift-cooling for the Upper Triassic series compared to other regions within the Weibei Uplift. Cooling paths for the Upper Triassic series became uniform regionally in the Early Cretaceous marking a key time for the tectonothermal evolutionary history of Upper Triassic series in the southwestern North China Plate. | Qiang Yu Zhanli Ren Rongxi Li Chung Ling Tao Ni Wanshan Lei Baojiang Wang Xiaoli Wu Xiaoli Qin Xianghe Lei | 2023 | Journal of Earth Science2023，34，5： | 0 |
| 15 | Selection of different endothelialization modes and different seed cells for tissue-engineered vascular graft显示文摘Tissue-engineered vascular grafts(TEVGs)have enormous potential for vascular replacement therapy.However,thrombosis and intimal hyperplasia are important problems associated with TEVGs especially small diameter TEVGs(<6 mm)after transplantation.Endothelialization of TEVGs is a key point to prevent thrombosis.Here,we discuss different types of endothelialization and different seed cells of tissue-engineered vascular grafts.Meanwhile,endothelial heterogeneity is also discussed.Based on it,we provide a new perspective for selecting suitable types of endothelialization and suitable seed cells to improve the long-term patency rate of tissue-engineered vascular grafts with different diameters and lengths. | Qingjin Cai Wanshan Liao Fangchao Xue Xiaochen Wang Weiming Zhou Yanzhao Li Wen Zeng | 2021 | Bioactive Materials2021，6，8： | 0 |
| 16 | STUDY ON COPYING QUALITY IN CONTOUR GRINDING显示文摘STUDYONCOPYINGQUALITYINCONTOURGRINDINGSTUDYONCOPYINGQUALITYINCONTOURGRINDINGWangWanshan;WangBo;LuShubin;ShanRuilan(Northeaste... | Wang Wanshan Wang Bo Lu Shubin Shan Ruilan(Northeastern University)Guo Changsheng(University of Massachusetts, U .S.A.) | 1995 | Chinese Journal of Mechanical Engineering1995，8，1： | 0 |
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