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| 1 | Epigenetic regulation of phosphodiesterase 4d in restrictive cardiomyopathy mice with cTnI mutations显示文摘Epigenetic regulations play an important role in disease development.In this study,we have investigated epigenetic regulations in restrictive cardiomyopathy mice with cTnI 193 His mutation.Our results demonstrated that phosphodiesterase(PDEs)4d was down-regulated in the heart of these mice.Further studies showed that the epigenetic modifications were associated with enhanced acetylation of histone 3 lysine 4 and lysines 9,whereas tri-methylation of histone 3 lysine 4,were decreased in histones near the PDE4d gene promoter regions.The binding levels of histone transmethylase SMYD1 and histone deacetylase HDAC1 were increased in the gene promoter regions in cTnI193 His transgenic hearts.Using immune-fluorescent labeling we found an evidence of cTnI existence in the nucleus of cardiomyocytes and Western blotting further confirmed that both wild type and mutated cTnI could be detected in the cell nucleus of the hearts.Furthermore,an interaction between cTnI and SMYD1,or cTnI and HDAC1 was observed.Overexpression of the mutated cTnI in cultured cardiomyocytes reduced the expression of PDE4d.Our data suggest that the decrease of PDE4d expression in RCM mice caused by cTnI mutations may be related to epigenetic regulation,i.e.,histone acetylation and methylation,and cTnI might be involved in this procedure via an interaction with HDAC1 and SMAD1 in the hearts. | Weian Zhao Xiaoqi Wu Zhiyuan Wang Bo Pan Lifei Liu Lingjuan Liu Xupei Huang Jie Tian | 2020 | Science China(Life Sciences)2020，63，4： | 4 |
| 2 | Hepatocyte Growth Factor-Loaded Biomaterials for Mesenchymal Stem Cell Recruitment显示文摘 | Julia van de Kamp Willi Jahnen-Dechent Bjoern Rath Ruth Knuechel Sabine Neuss Weian Zhao | 2013 | Stem Cells International2013，，： | 1 |
| 3 | Nanoparticle-based monitoring of cell therapy显示文摘 | Chenjie Xu Luye Mu Isaac Roes David Miranda-Nieves Matthias Nahrendorf James A Ankrum Weian Zhao Jeffrey M Karp | 2011 | Nanotechnology2011，，49： | 1 |
| 4 | Mesenchymal stem cells engineered to express selectin ligands and IL-10 exert enhanced therapeutic efficacy in murine experimental autoimmune encephalomyelitis显示文摘 | Wenbin Liao Victor Pham Linan Liu Milad Riazifar Egest J. Pone Shirley Xian Zhang Fengxia Ma Mengrou Lu Craig M. Walsh Weian Zhao | 2016 | Biomaterials2016，，： | 1 |
| 5 | Synthesis, characterization and evaluation of a quadripolymer with lowmolecular weight as a water based drilling fluid viscosity reducer at high temperature (245 ℃ ) 显示文摘 | Huang Weian Zhao Cong Qiu Zhengsong | 2015 | Polymer International2015，64，： | 1 |
| 6 | Effects of different kinds of clay and different vinyl acetate content on the morphology and properties of EVA/clay nanocomposites 显示文摘 | ZHANG Weian CHEN Dazhu ZHAO Quanbao | 2003 | Polymer2003，44，26： | 1 |
| 7 | Engineered mesenchymal stem cells with self-assembled vesicles for systemic cell targeting显示文摘 | Debanjan Sarkar Praveen K. Vemula Weian Zhao Ashish Gupta Rohit Karnik Jeffrey M. Karp | 2010 | Biomaterials2010，，19： | 1 |
| 8 | Cardiac troponin I R193H mutant interacts with HDAC1 to repress phosphodiesterase 4D expression in cardiomyocytes显示文摘Cardiac Troponin I(cTnI)is a subunit of the thin filament involved in regulation of heart contraction.Mutated cTnI accounts for most genetic mutations associated with restric-tive cardiomyopathy(RCM).We previously found phosphodiesterase 4D(PDE4D)decreased in RCM mice with cTnIR193H mutation and the mutant cTnI might be involved in PDE4D reduction.This study aims to elucidate a novel role of cTnIR193H mutant as a gene regulator.Overexpres-sion of cTnIR193H mutant in cardiomyocytes showed decrease in PDED4D protein expression,while the enrichment of histone deacetylase 1(HDAC1)was increased along with decreases in acetylated lysine 4(acH3K4)and 9(acH3K9)levels in the PDE4D promoter.HDAC1 overex-pression could also downregulate PDE4D via reducing acH3K4 and acH3K9 levels.Co-IP assays showed that cTnIR193H mutant owed increased binding ability to HDAC1 compared with wild type cTnI.EGCG as a HDAC1 inhibitor could diminish the strength of cTnIR193H-HDAC1 inter-actions and alleviate the reduction in PDE4D expression.Together,our data indicated that cTnIR193H mutant could repress PDE4D expression in cardiomyocytes through HDAC1 associ-ated histone deacetylation modification.Unlike the typical function of cTnI in cytoplasm,our study suggested a novel role of cTnI mutants in nuclei in regulating gene expression. | Weian Zhao Qian lu Jing Luo Bo Pan Ling-Juan Liu Jie Tian | 2021 | Genes & Diseases2021，8，4： | 0 |
| 9 | Inference for constant-stress accelerated life test with Type-I progressively hybrid censored data from Burr-XII distribution显示文摘This paper proposes a simple constant-stress accelerated life test(ALT) model from Burr type XII distribution when the data are Type-I progressively hybrid censored. The maximum likelihood estimation(MLE) of the parameters is obtained through the numerical method for solving the likelihood equations. Approximate confidence interval(CI), based on normal approximation to the asymptotic distribution of MLE and percentile bootstrap CI is derived. Finally, a numerical example is introduced and then a Monte Carlo simulation study is carried out to illustrate the proposed method. | Jiao Zhao Yimin Shi Weian Yan | 2014 | Journal of Systems Engineering and Electronics2014，25，2： | 0 |
| 10 | New Story of Red River Valley显示文摘Legend has it that it was some 500 years ago when the local leader Qoigyi Raodun Guisang Parba built on Gyangze's Zongshan Hill the first palace called Gyikarze, from which the county's name is derived. | ZHAO WEIAN | 2004 | China's Tibet2004，15，3： | 0 |
| 11 | Intranuclear cardiac troponin I plays a functional role in regulating Atp2a2 expression in cardiomyocytes显示文摘In the past studies,it is shown that cardiac troponin I(cTnI,encoded by TNNI3),as a cytoplasmic protein,is an inhibitory subunit in troponin complex,and involves in cardiomyocyte diastolic regulation.Here,we assessed a novel role of cTnI as a nucleoprotein.Firstly,the nuclear translocation of cTnI was found in mouse,human fetuses and rat heart tissues.In addition,there were differences in percentage of intranuclear cTnI in different conditions.Based on weighted gene co-expression network analyses(WGCNA)and verification in cell experiments,a strong expression correlation was found between TNNI3 and Atp2a2,which encodes sarco-endoplasmic reticulum Ca2t ATPase isoform 2a(SERCA2a),and involves in ATP hydrolysis and Ca2t transient.TNNI3 gain and loss caused Atpa2a2 increase/decrease in a dosedependent manner both in mRNA and protein levels,in vivo and in vitro.By using ChIP-sequence we demonstrated specific binding DNA sequences of cTnI were enriched in ATP2a2 promoter239we889 region and the specific binding sequence motif of cTnI was analyzed by software as'CCAT',which has been reported to be required for YY1 binding to the promoter region of YY1-related genes.Moreover,it was further verified that pcDNA3.1()-TNNI3 could express cTnI proteins and increase the promoter activity of Atp2a2 through luciferase report assay.In the end,we evaluated beat frequencies,total ATP contents,Ca2t transients in TNNI3-siRNA myocardial cells.These findings indicated,for the first time,cTnI may regulate Atp2a2 in cardiomyocytes as a co-regulatory factor and participate in the regulation of intracellular Ca ions. | Qian Lu Bo Pan Haobo Bai Weian Zhao Lingjuan Liu Gu Li Ruimin Liu Tiewei Lv Xupei Huang Xi Li Jie Tian | 2022 | Genes & Diseases2022，9，6： | 0 |
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