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| 1 | The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism显示文摘在动物农业和药的抗菌素的 overuse 引起了一系列潜在的威胁到公共健康。Macleaya cordata 是从 Papaveraceae 家庭的药用的植物种,提供为抗菌剂的制造的一个安全资源为家畜喂添加剂。从 M. 的活跃成分 ? cordata 被知道包括 benzylisoquinoline 碱(偏爱) 象 sanguinarine (圣) 和 chelerythrine (CHE ) 那样的 ?,而是他们的新陈代谢的小径还得在这非模型植物被学习。在 M. 的圣和 CHE 的活跃生合成 ? cordata 被喂 13标记 C 的酷氨酸。为了增加,推进卓见,我们 de novo 定序 M 的整个染色体。cordata,第一从 Papaveraceae 家庭被定序。M.? 盖住 378 Mb 的 cordata 染色体与 43.5%being 编码 22,328 预言的编码蛋白质的基因 transposable 元素。作为基础 eudicot 的一个成员, M.? cordata 染色体缺乏 ? 发生在几乎所有 eudicots 的 paleohexaploidy 事件。从 genomics 数据, 16 的一个完全的集合 ? 新陈代谢 ? 为圣和 CHE 生合成的基因被检索,并且 14 项他们的生物化学的活动被验证。这些 genomics 和新陈代谢的数据在 M. 显示出保存 BIA 新陈代谢的小径 ? cordata 并且由庄稼改进或微生物引起的小径重建为圣和 CHE 的未来生产提供知识基础。 | Xiubin Liu Yisong Liu Peng Huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang Yixiu Zhuo Yancong Zhang Linlan Yu Jialu Huang Peng Yang Qiong Peng dinbo Zhang Wenkai Jiang Zhonghua Zhang Kui Lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong Yi Shang Sanwen Huang Jianguo Zeng | 2017 | Molecular Plant2017，10，7： | 19 |
| 2 | A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis显示文摘Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur compounds.Here,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced genome.Analysis of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic genome.We examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two compounds.Furthermore,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged bulbs.The reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding. | Xiudong Sun Siyuan Zhu Ningyang Li Yi Cheng Jing Zhao Xuguang Qiao Li Lu Shiqi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo Shuang Gao Zemao Yang Fu Li Zheng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian Zhao Xiaomi ng Lu Huanwe n Meng Zhenlin Han Chun she ng Gao Wenkai Jiang Xing Zhao Shilin Tian Jianguang Su Zhihui Cheng Touming Liu | 2020 | Molecular Plant2020，13，9： | 14 |
| 3 | Cloning, expression and protective immunity evalua-tion of the full-length cDNA encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum显示文摘1071-bp fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the 3′ and 5′ ends of the incomplete expression sequence tag (EST) of succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) were amplified by the anchored PCR with 2 pairs of primers designed according to the EST of SjSDISP and the sequence of multiclone sites of the library vector. Sequence analysis indicated that the fragment was a full-length cDNA with a complete open reading frame (ORF), encoding 278 amino acid residues. The fragment was cloned into prokary- otic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE and Western-blot analyses showed that the recombinant protein was about 32 kD and could be recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Compared with the FCA controls, mice vaccinated with rSjSDISP (test) or rSjGST (posi- tive control) all revealed high levels of specific antibody and significant reduction in worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs. These results suggest that SjSDISP may be a novel and partially protective vaccine candidate against schistosomiasis. In contrast to the worm burden reduction rate, the higher degree of egg reduction rate in the test group also sug- gested that SjSDISP vaccine may primarily play a role in anti-embryonation or anti-fecundity immunity. | YU JunLong, WANG ShiPing, LI WenKai, DAI Gan, XU ShaoRui, HE Zhuo, PENG XianChu, ZHOU SongHua & LIU XueQin Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China | 2007 | Science China(Life Sciences)2007，50，2： | 4 |
| 4 | Side-chain modification of polyethylene glycol on conjugated polymers for ternary blend all-polymer solar cells with efficiency up to 9.27%显示文摘With the rapid progress achieved by all-polymer solar cells(all-PSCs), wide-bandgap copolymers have attracted intensive attention for their unique advantage of constructing complementary absorption profiles with conventional narrow-bandgap copolymers. In this work, we designed and synthesized a wide bandgap ternary copolymer PEG-2% which has the benzodithiophene-alt-difluorobenzotriazole as the backbone and the polyethylene glycol(PEG) modified side chain. The PBTA-PEG-2%:N2200 can be processed with a non-chlorinated solvent of 2-methyl-tetrahydrofuran(MeTHF) for the binary all-PSC, which exhibits a moderate photovoltaic performance. In particular, the ternary all-PSCs that consisting an additional narrow bandgap polymer donor PTB7-Th can also be processed with MeTHF, resulting in an unprecedented power conversion efficiency(PCE)of 9.27%, and a high PCE of 8.05% can be achieved with active layer thickness of 240 nm, both of which are the highest values so far reported from all-PSCs. Detailed investigations revealed that the dramatically improved device performances are attributable to the well-extended absorption band in the photoactive layer. Hence,developing novel copolymers with tailored side chains, and introducing additional polymeric components, can broaden the horizon for high-performance all-PSCs. | Zhenye Li Baobing Fan Baitian He Lei Ying Wenkai Zhong Feng Liu Fei Huang Yong Cao | 2018 | Science China Chemistry2018，61，4： | 4 |
| 5 | Association between arterial stiffness and risk of coronary artery disease in a community-based population显示文摘 | Zhang Yun Ye Ping Luo Leiming Bai Yongyi Xu Ruyi Xiao Wenkai Liu Dejun Wu Hongmei | 2014 | Chinese Medical Journal2014，，22： | 3 |
| 6 | Development of a SYBR-based real-time PCR to detect rabbit hemorrhagic disease virus(RHDV) and analyze its tissue distribution in experimentally infected rabbits显示文摘Dear Editor,Rabbit hemorrhagic disease(RHD)is a highly contagious disease of both wild and domesticated rabbits(Oryctolagus cuniculus).The causative agent of the disease is the rabbit hemorrhagic disease virus(RHDV),belongs to the genus Lagovirus within the family Caliciviridae(Granzow et al.,1996;Ohlinger et al.,1990).It is a small and non-enveloped virus with a 7.5 kb single stranded positive sense RNA genome(Meyers et al.,1991;Meyers et al.,2000).Based on an analysis of | Wenkai Liu Ruyi Dang Xinglong Wang | 2015 | Virologica Sinica2015，30，3： | 3 |
| 7 | The Environmental Functions and Ecological Effects of Organic Carbon in Silt显示文摘Silt is a kind of unconsolidated sediment consisting of fine particles;silt is generally deposited across wide areas on the surfaces of drainages and in oceans under static or slow-hydrodynamic conditions. The organic carbon(OC) in silt has multiple essential environmental functions. This paper elaborates the morphological and environmental indication functions of OC in silt, and the effect of its own migration and transformation on environmental deterioration. Organic carbon exists in silt in two forms, free and mineral-binding. Meanwhile, based on its formation and structure, OC can be divided into light and heavy fraction of OC. Environmental information including data related to paleoclimates, ancient levels of productivity level, and variations in regional organism abundance can be discovered from total organic carbon, the C/N ratio, and OC isotope content. Degradation of OC is believed to participate in the emission of greenhouse gases, release of heavy metals and other contaminants. Finally, from the view of silt deposition, the possible influence of complex water-rock interaction in which OC is involved during the evolution of silt to a clayey aquitard on the hydrochemical composition of groundwater is discussed, which provides a new perspective for future research on the carbon cycle in nature. | Rui Liu Teng Ma Wenkai Qiu Ziqi Peng Chenxuan Shi | 2020 | Journal of Earth Science2020，31，4： | 2 |
| 8 | Melon short internode(CmSi)encodes an ERECTA-like receptor kinase regulating stem elongation through auxin signaling显示文摘Plant height is one of the most important agronomic traits that directly determines plant architecture,and compact or dwarf plants can allow for increased planting density and land utilization as well as increased lodging resistance and economic yield.At least four dwarf/semidwarf genes have been identified in different melon varieties,but none of them have been cloned,and little is known about the molecular mechanisms underlying internode elongation in melon.Here,we report map-based cloning and functional characterization of the first semidwarf gene short internode(Cmsi)in melon,which encodes an ERECTA-like receptor kinase regulating internode elongation.Spatial-temporal expression analyses revealed that CmSI exhibited high expression in the vascular bundle of the main stem during internode elongation.The expression level of CmSI was positively correlated with stem length in the different melon varieties examined.Ectopic expression of CmSI in Arabidopsis and cucumber suggested CmSI as a positive regulator of internode elongation in both species.Phytohormone quantitation and transcriptome analysis showed that the auxin content and the expression levels of a number of genes involved in the auxin signaling pathway were altered in the semidwarf mutant,including several well-known auxin transporters,such as members of the ABCB family and PINFORMED genes.A melon polar auxin transport protein CmPIN2 was identified by protein–protein interaction assay as physically interacting with CmSI to modulate auxin signaling.Thus,CmSI functions in an auxin-dependent regulatory pathway to control internode elongation in melon.Our findings revealed that the ERECTA family gene CmSI regulates stem elongation in melon through auxin signaling,which can directly affect polar auxin transport. | Sen Yang Kaige Zhang Huayu Zhu Xiaojing Zhang Wenkai Yan Nana Xu Dongming Liu Jianbin Hu Yufeng Wu Yiqun Weng Luming Yang | 2020 | Horticulture Research2020，7，1： | 2 |
| 9 | Quantitative evaluation of heavy metals’ pollution hazards in liquefaction residues of sewage sludge显示文摘 | Huajun Huang Xingzhong Yuan Guangming Zeng Huina Zhu Hui Li Zhifeng Liu Hongwei Jiang Lijian Leng Wenkai Bi | 2011 | Bioresource Technology2011，，22： | 2 |
| 10 | Design of intelligent vehicle system basedon embedded ARM显示文摘 | LIU Wenkai XU Aigong WU Menglong | 2014 | WITPress2014，51，： | 1 |
| 11 | A Downhill Brake Strategy Focusing on Temperature and Wear Loss Control of Brake System显示文摘 | Liu Zongyu Zheng Hongyu Xu Wenkai | 2013 | SAE Paper2013，，： | 1 |
| 12 | 13.4 fs,0.1 Hz OPCPA Front End for the 100 PW-Class Laser Facility显示文摘Here,we report the recent progress on the front end developed for the 100 PW-class laser facility.Using 3 stages of optical parametric chirped-pulse amplification(OPCPA)based on lithium triborate(LBO)crystals,we realized a 5.26 J/0.1 Hz amplified output with a bandwidth over 200 nm near the center wavelength of 925 nm.After the compressor,we obtained a pulse duration of 13.4 fs.As the compression efficiency reached 67%,this OPCPA front end could potentially support a peak power of 263 TW at a repetition rate of 0.1 Hz.To the best of our knowledge,among all the 100 TW-level OPCPA systems,it shows the widest spectral width,the shortest pulse duration,and it is also the first OPCPA system working at a repetition-rate mode. | Xinliang Wang Xingyan Liu Xiaoming Lu Junchi Chen Yingbin Long Wenkai Li Haidong Chen Xun Chen Peile Bai Yanyan Li Yujie Peng Yanqi Liu Fenxiang Wu Cheng Wang Zhaoyang Li Yi Xu Xiaoyan Liang Yuxin Leng Ruxin Li | 2022 | Ultrafast Science2022，，3： | 1 |
| 13 | Association Between Tp‐e/QT Ratio and Prognosis in Patients Undergoing Primary Percutaneous Coronary Intervention for ST‐Segment Elevation Myocardial Infarction显示文摘 | Xiangmei Zhao Zhouliang Xie Yingjie Chu Lei Yang Wenkai Xu Xianzhi Yang Xiaoyu Liu Lixiao Tian | 2012 | Clin Cardiol2012，，9： | 1 |
| 14 | Particle-in-cell simulation of the focusing properties of high power electron beam in plasma ion channel显示文摘 | Meng Lin Xie Wenkai Liu Shenggang | 1999 | Int J of IR/MM Waves1999，20，8： | 1 |
| 15 | Feature Alignment Approach for Hand Posture Recognition Based on Curvature Scale Space显示文摘 | Chang C C Liu Chengyi Tai Wenkai | 2008 | Neurocomputing2008，71，1012： | 1 |
| 16 | Motion detection for human bodies basing adaptive background subtraction by using an omni-directional camera显示文摘 | Pi Wenkai Liu Hong Cha Hongbin | 2004 | Acta Scientiarum Naturalium Univeraitatis Pekinensis2004，40，3： | 1 |
| 17 | Characterizing the influence of gut microbiota on host tryptophan metabolism with germ-free pigs显示文摘Intestinal microbes are closely associated with host health,depending on metabolic crosstalk between the microbiota and host.Tryptophan metabolism is one of the best examples of metabolic crosstalk between intestinal microbiota and host;however,our understanding about the influence of intestinal microbiota on host tryptophan metabolism is limited.Thus,we established germ-free(GF)pig models to systemically explore the influence of intestinal microbiota on tryptophan metabolism.Five GF pigs were kept in GF conditions throughout the experiment(GF group).Six GF pigs were transplanted with fecal microbiota from donor sows to act as control pigs.Compared with control pigs,the GF pigs had remarkable alterations in tryptophan metabolism.The differential metabolites(P<0.05)were mainly found in the liver,circulation system and large intestine.Notably,the alteration of metabolites in tryptophan metabolism varied among organs,especially for the serotonin pathway.In GF pigs,tryptophan and kynurenine in the large intestine and 5-hydroxytryptophan in most organs were increased(P<0.05),while metabolites in the indole pathway in most organs were decreased(P<0.05).Collectively,our study reveals changes in tryptophan metabolism in GF pigs,highlighting the critical role of gut microbes in shaping host tryptophan metabolism. | Bingnan Liu Dongming Yu Jing Sun Xiaoyan Wu Zhongquan Xin Baichuan Deng Lijuan Fan Jian Fu Liangpeng Ge Wenkai Ren | 2022 | Animal Nutrition2022，，4： | 1 |
| 18 | Research on the process of fabricating a multi-layer metal micro-structure based on UV-LIGA overlay technology显示文摘In this paper,we report the study of the process of fabricating a multi-layermetal micro-structure using UV-LIGA overlay technology,includingmask fabrication,substrate treatment,and UV-LIGA overlay processes.To solve the process problems in the masking procedure,the swelling problemof the first layer of SU-8 thick photoresist was studied experimentally.The 5μmline-width compensation and closed 20μmand 30μmisolation strips were designed and fabricated around the micro-structure pattern.The pore problemin the Ni micro-electroforming layer was analyzed and the electroforming parameters were improved.The pH value of the electroforming solution should be controlled between 3.8 and 4.4 and the current density should be below 3 A/dm^2.To solve the problems of high inner stress and incomplete development of the micro-cylinder hole array with a diameter of 30μm,the lithography process was optimized.The pre-baking temperature was increased via gradient heating and rose every 5℃ from 65℃ to 85℃ and then remained at 85℃ for 50 min–1 h.In addition,the full contact exposure was used.Finally,a multi-layer metal micro-structure with high precision and good quality of microelectroforming layer was fabricated using UV-LIGA overlay technology. | Yali Ma Wenkai Liu Chong Liu | 2019 | Nanotechnology and Precision Engineering2019，2，2： | 1 |
| 19 | Preliminary report of the September 5,2022 M_(S) 6.8 Luding earthquake,Sichuan,China显示文摘The 2022 M_(S)6.8 Luding earthquake is the strongest earthquake in Sichuan Province, Western China, since the 2017 M_(S)7.0 Jiuzhaigou earthquake. It occurred on the Moxi fault in the southeastern segment of the Xianshuihe fault, a tectonically active and mountainous region with severe secondary earthquake disasters. To better understand the seismogenic mechanism and provide scientific support for future hazard mitigation, we summarize the preliminary results of the Luding earthquake, including seismotectonic background, seismicity and mainshock source characteristics and aftershock properties, and direct and secondary damage associated with the mainshock.The peak ground displacements in the NS and EW directions observed by the nearest GNSS station SCCM are ~35 mm and ~55 mm, respectively, resulting in the maximum coseismic dislocation of 20 mm along the NWW direction, which is consistent with the sinistral slip on the Xianshuihe fault. Back-projection of teleseismic P waves suggest that the mainshock rupture propagated toward south-southeast. The seismic intensity of the mainshock estimated from the back-projection results indicates a Mercalli scale of Ⅷ or above near the ruptured area,consistent with the results from instrumental measurements and field surveys. Numerous aftershocks were reported, with the largest being M_(S)4.5. Aftershock locations(up to September 18, 2022) exhibit 3 clusters spanning an area of 100 km long and 30 km wide. The magnitude and rate of aftershocks decreased as expected, and the depths became shallower with time. The mainshock and two aftershocks show left-lateral strike-slip focal mechanisms. For the aftershock sequence, the b-value from the Gutenberg-Richter frequency-magnitude relationship, h-value, and p-value for Omori’s law for aftershock decay are 0.81, 1.4, and 1.21, respectively, indicating that this is a typical mainshock-aftershock sequence. The low b-value implies high background stress in the hypocenter region. Analysis from remote sensing satellite images and UAV data shows that the distribution of earthquake-triggered landslides was consistent with the aftershock area. Numerous small-size landslides with limited volumes were revealed, which damaged or buried the roads and severely hindered the rescue process. | Yanru An Dun Wang Qiang Ma Yueren Xu Yu Li Yingying Zhang Zhumei Liu Chunmei Huang Jinrong Su Jilong Li Mingxiao Li Wenkai Chen Zhifan Wan Dengjie Kang Baoshan Wang | 2023 | Earthquake Research Advances2023，3，1： | 1 |
| 20 | Research on etch rate of reactive ion etching of GaAs,AlAs and DBR显示文摘 | Lin Shiming Wu Shu | 2001 | Chinese Journal of Semiconductors2001，22，9： | 1 |
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