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| 1 | Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice显示文摘Phosphorus (P) deficiency is a major limitation for plant growth and development. Among the wide set of responses to cope with low soil P, plants increase their level of intracellular and secreted acid phosphatases (APases), which helps to catalyze inorganic phosphate (Pi) hydrolysis from organophosphates. In this study we characterized the rice (Oryza sativa) purple acid phosphatase 10a (OsPAP10a). OsPAP10a belongs to group Ia of purple acid phosphatases (PAPs), and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis. The transcript abundance of OsPAP10a is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation. Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root-associated APase. Furthermore, overexpression of OsPAP10a significantly improved ATP hydrolysis and utilization compared with wild type plants. These results indicate that OsPAP10a can potentially be used for crop breeding to improve the efficiency of P use. | Jingluan Tian Chuang Wang Qian Zhang Xiaowei He James Whelan Huixia Shou | 2012 | Journal of Integrative Plant Biology2012，54，9： | 18 |
| 2 | Colorectal cancer in patients under 50 years of age:A retrospective analysis of two institutions' experience显示文摘AIM:To investigate the epidemiological characteristics of colorectal cancer(CRC)in patients under 50 years of age across two institutions.METHODS:Records of patients under age 50 years of age who had CRC surgery over a 16 year period were assessed at two institutions.The following documents where reviewed:admission notes,operative notes,and discharge summaries.The main study variables included:age,presenting symptoms,family history,tumor location,operation,stage/differentiation of disease,and post operative complications.Stage of disease was classified according to the American Joint Committee on Cancer TNM staging system:tumor depth;node status;and metastases.RESULTS:CRC was found in 180 patients under age50 years(87 females,93 males;mean age 41.4±6.2years).Young patients accounted for 11.2%of cases during a 6 year period for which the full data set wasavailable.Eight percent had a 1stdegree and 12%a 2nd degree family CRC history.Almost all patients(94%)were symptomatic at diagnosis;common symptoms included:bleeding(59%),obstruction(9%),and abdominal/rectal pain(35%).Evaluation was often delayed and bleeding frequently attributed to hemorrhoids.Advanced stage CRC(Stage 3 or 4)was noted in 53%of patients.Most tumors were distal to the splenic flexure(77%)and 39%involved the rectum.Most patients(95%)had segmental resections;6 patients had subtotal/total colectomy.Poorly differentiated tumors were noted in 12%and mucinous lesions in 19%of patients of which most had Stage 3 or 4 disease.Twenty-two patients(13%)developed recurrence and/or progression of disease to date.Three patients(ages 42,42and 49 years)went on to develop metachronous primary colon cancers within 3 to 4 years of their initial resection.CONCLUSION:CRC was common in young patients with no family history.Young patients with symptoms merit a timely evaluation to avoid presentation with late stage CRC. | Elizabeth A Myers Daniel L Feingold Kenneth A Forde Tracey Arnell Joon Ho Jang Richard L Whelan | 2013 | World Journal of Gastroenterology2013，19，34： | 9 |
| 3 | Systematic review: faecal microbiota transplantation in the management of inflammatory bowel disease显示文摘 | J. L. Anderson R. J. Edney K. Whelan | 2012 | Aliment Pharmacol Ther2012，，6： | 5 |
| 4 | Inactivation of Mitochondrial Complex Ⅰ Induces the Expression of a Twin Cysteine Protein that Targets and Affects Cytosolic, Chloroplastidic and Mitochondrial Function显示文摘At12Cys-1 (At5g64400 ) 和 At12Cys-2 (At5g09570 ) 是二密切相关的 isogenes 编码小、成双的半胱氨酸蛋白质,典型地在线粒体定位了。At12Cys-2 抄本与破坏 mitochondrial 蛋白质,而是 At12Cys 的增加在许多异种被导致蛋白质仅仅与减少的 mitochondrial 在异种被检测复杂我丰富。在缺乏 mitochondrial 建筑群的异种的 At12Cys 蛋白质感应我被位于线粒体,叶绿体,和 cytosol 的 At12Cys 蛋白质伴随。生物化学的分析揭示了那甚至单个的基因删除,即, At12cys-1 或 At12cys-2,在 mitochondrial 和叶绿体功能上有效果。然而,仅仅加倍异种,即, At12cys-1 : At12cys-2,影响许多蛋白质和 mRNA 抄本编码翻译延伸因素以及 rRNA 丰富。蓝本国的页证明 At12Cys 与 mitochondrial supercomplex 共同移居我 + III。同样, At12cys-1 和 At12cys-2 基因的删除,然而并非单个基因删除,导致提高的忍耐到干旱和轻应力和增加的抗氧化剂能力。正式就职和在在建筑群的减小之上的 At12Cys 的多重本地化我丰富提供机制明确地表明 mitochondrial 机能障碍到 cytosol 然后在以外到在房间的另外的细胞器。 | Yan Wang Wenhui Lyu Oliver Berkowitz Jordan D. Radomiljac Simon R. Law Monika W. Murcha Chris Carrie Pedro F. Teixeira Beata Kmiec Owen Duncan Olivier Van Aken Reena Narsai Elzbieta Glaser Shaobai Huang Ute Roessner A. Harvey Millar James Whelan | 2016 | Molecular Plant2016，9，5： | 5 |
| 5 | 采用里卡多高速直喷式4气门发动机以满足低排放和燃油经济性的要求显示文摘为了研究和证明高速直喷式柴油机的潜力,里卡多公司实施了一项研究并分析每缸4气门燃烧系统性能和排放的计划。分析研究以计算流体力学的研究为基础,以模拟活塞顶燃烧室凹腔内油束与空气的相互作用,并加深对混合过程的理解。试验研究的主要变量包括涡流比、燃烧室凹腔的几何形状、喷油嘴的结构型式、当代先进的转子式电控分配泵和废气再循环的作用。系统的优化过程提供一种固定不变的涡流型式,其制动平均有效压力可达1.5 MPa[15 bar]以上,而BSU(波许烟度单位)却在1.0以下。R49欧洲Ⅱ NO_X水平满足烟度、颗粒排放量和燃油经济性的竞争要求,证明完全按今后发展的欧洲Ⅱ具有极大的开发潜力。车辆排放和燃油耗的模拟表明,此燃烧系统具有满足90年代规定的重型和轻型车排放法规的潜力。 | Needham J R Whelan S 张耀庆 | 1996 | 国外内燃机1996，28，2： | 5 |
| 6 | Defining the Mitochondrial Stress Response in Arabidopsis thaliana显示文摘获得的全球概述线粒体对应力作出回应,我们试图定义工厂 mitochondrial 压力反应(MSR ) 。由联合通常认为地与压力相关的条件的 16 个 microarray 实验编码 mitochondrial 蛋白质的一套 1196 Arabidopsis thaliana 基因, 45 原子编码基因被定义为广泛地压力应答。用绿色荧光灯蛋白质(GFP ) 熔化试金,很多这些指向蛋白质的 mitochondrial 被测试,在是的总数 26 蛋白质证实 mitochondrially 指向了。几这些蛋白质被观察双指向了到线粒体和质体,包括小热吃惊蛋白质 sHSP23.5 和 sHSP23.6。除了线粒体的明确的压力部件,例如其他的 oxidases,菸碱腺嘌 dinucleotide (NAD (P) H ) 脱氢酶,和热震惊蛋白质,许多另外的蛋白质,许多与未知功能,被识别。mitochondrial 搬运人蛋白质家庭在压力应答的基因是过去代表的,建议那个压力越过 mitochondrial 为代谢物运输导致改变的需要内部膜。尽管这些蛋白质编码许多的基因包含普通行动 cis 规章的元素,很多个不同规章的过程或信号多半触发了 MSR,是明显的。因此,这些基因提供新模型系统学习 mitochondrial 后退规定,除了广泛地使用的其他的 oxidase 模型。另外,当在对应力应答的蛋白质的变化没在一个抄本与变化相关很好,铺平,它建议 post-transcriptional 机制也在定义 MSR 起一个重要作用。 | Olivier Van Aken Botao Zhang Chris Carrie Vindya Uggalla Ellen Paynter Estelle Giraud James Whelan | 2009 | Molecular Plant2009，2，6： | 4 |
| 7 | Plasma chitinase 3-like 1 is persistently elevated during first month after minimally invasive colorectal cancer resection显示文摘AIM: To assess blood chitinase 3-like 1(CHi3L1) levels for 2 mo after minimally invasive colorectal resection(MICR) for colorectal cancer(CRC). METHODS: CRC patients in an Institutional Review Board approved data/plasma bank who underwent elective MICR for whom preoperative(PreO p), early postoperative(PostO p), and 1 or more late PostO p samples [postoperative day(POD) 7-27] available were included. Plasma CHi3L1 levels(ng/m L) were determined in duplicate by enzyme linked immunosorbent assay. RESULTS: PreOp and PostOp plasma sample were available for 80 MICR cancer patients for the study. The median PreOp CHi3L1 level was 56.8 CI: 41.9-78.6 ng/mL(n = 80). Significantly elevated(P < 0.001) median plasma levels(ng/mL) over PreOp levels were detected on POD1(667.7 CI: 495.7, 771.7; n = 79), POD 3(132.6 CI: 95.5, 173.7; n = 76), POD7-13(96.4 CI: 67.7, 136.9; n = 62), POD14-20(101.4 CI: 80.7, 287.4; n = 22), and POD 21-27(98.1 CI: 66.8, 137.4; n = 20, P = 0.001). No significant difference in plasma levels were noted on POD27-41. CONCLUSION: Plasma CHi3L1 levels were significantly elevated for one month after MICR. Persistently elevated plasma CHi3L1 may support the growth of residual tumor and metastasis. | HMC Shantha Kumara David Gaita Hiromichi Miyagaki Xiaohong Yan Sonali AC Hearth Linda Njoh Vesna Cekic Richard L Whelan | 2016 | World Journal of Gastrointestinal Oncology2016，8，8： | 4 |
| 8 | 多土壤信息测量传感器的硬件系统集成设计(英文)显示文摘土壤在作物的生长过程中起到了重要的作用,所以对于基于决策的作物生产管理,土壤信息是必需的。传统的土壤取样获取土壤信息技术耗时且成本高,尤其是对于大规模农田土壤信息测量。目前一些近地面的可连续测量的土壤信息传感器技术能够提供高精度的数字土壤信息地图,然而这些商业化的技术成熟的传感器通常需要单独使用。该文提出了将γ射线光谱仪GR320、利用电磁感应原理的EM38和EM31以及Veris3100和VerispH这些可在农田近地面连续测量的土壤特性测试传感仪器集成在一起同时使用的方案,介绍了此集成系统的硬件设备和相关特性参数以及今后需要继续研究解决的问题。利用该系统可一次获得不同的土壤特性参数数据,如土壤矿物质含量,不同深度的土壤电导率值和土壤pH值等,可避免多次测量车辆行走对土壤的压实。多传感器数据之间的互相补充可以进一步提高且更有利于精确农业中基于土壤信息的决策规划。该系统适用于大面积农田土壤特性测量。 | 马瑞峻 Michael Short Craig Lobsey Alex McBramey Brett Whelan Budiman Minasny 孙光永 | 2010 | 农业工程学报2010，26，4： | 3 |
| 9 | Plasma MMP-2 and MMP-7 levels are elevated first month after surgery and may promote growth of residual metastases显示文摘BACKGROUND MMP-2 also known as gelatinase A and MMP-7(matrilysin)are members of the zinc-dependent family of MMPs(Matrix metalloproteinase).MMP-2 and MMP-7 are remodeling enzymes that digest extracellular matrix;MMP-2 is extensively expressed during development and is upregulated at sites of tissue damage,inflammation,and in stromal cells of metastatic tumors.MMP-7 is expressed in the epithelial cells and in a variety of cancers including colon tumors.Plasma MMP-2 and MMP-7 levels were assessed before and after minimally invasive colorectal resection for cancer pathology.AIM To determine plasma MMP-2 and MMP-7 levels before and after minimally invasive colorectal resection for cancer pathology.METHODS Patients enrolled in a plasma bank for whom plasma was available were eligible.Plasma obtained from preoperative(Preop)and postoperative blood samples was used.Only colorectal cancer(CRC)patients who underwent elective minimally invasive cancer resection with preop,post-operative day(POD)1,3 and at least 1 late postop sample(POD 7-34)were included.Late samples were bundled into 7 d blocks(POD 7-13,14-20,etc.)and treated as single time points.Plasma MMP-2 and MMP-7 levels were determined via enzyme-linked immunosorbent assay in duplicate.RESULTS Total 88 minimally invasive CRC resection CRC patients were studied(right colectomy,37%;sigmoid,24%;and LAR/AR 18%).Cancer stages were:1,31%;2,30%;3,34%;and 4,5%.Mean Preop MMP-2 plasma level(ng/mL)was 179.3±40.9(n=88).Elevated mean levels were noted on POD1(214.3±51.2,n=87,P<0.001),POD3(258.0±63.9,n=80,P<0.001),POD7-13(229.9±62.3,n=65,P<0.001),POD 14-20(234.9±47.5,n=25,P<0.001),POD 21-27(237.0±63.5,n=17,P<0.001,)and POD 28-34(255.4±59.7,n=15,P<0.001).Mean Preop MMP-7 level was 3.9±1.9(n=88).No significant differences were noted on POD 1 or 3,however,significantly elevated levels were noted on POD 7-13(5.7±2.5,n=65,P<0.001),POD 14-20(5.9±2.5,n=25,P<0.001),POD 21-27(6.1±3.6,n=17,P=0.002)and on POD 28-34(6.8±3.3,n=15 P<0.001,)vs preop levels.CONCLUSION MMP-2 levels are elevated for 5 wk and MMP-7 levels elevated for weeks 2-6.The etiology of these changes in unclear,trauma and wound healing likely play a role.These changes may promote residual tumor growth and metastasis. | HMC Shantha Kumara Hiromichi Miyagaki Sajith A Herath Erica Pettke Xiaohong Yan Vesna Cekic Richard L Whelan | 2021 | World Journal of Gastrointestinal Oncology2021，13，8： | 3 |
| 10 | Smoking in inflammatory bowel disease: Impact on disease course and insights into the aetiology of its effect显示文摘 | Gareth C. Parkes Kevin Whelan James O. Lindsay | 2014 | Journal of Crohn’s and Colitis2014，，： | 3 |
| 11 | Probiotics in the management of irritable bowel syndrome and inflammatory bowel disease显示文摘 | Kevin Whelan Eamonn M.M. Quigley | 2013 | Current Opinion in Gastroenterology2013，，2： | 3 |
| 12 | 克罗恩病患者使用低聚果糖的临床、微生物学和免疫学效果显示文摘Background and aims: The intestinal microbiota play a pivotal role in the inflammation associated with Crohn’s disease through their interaction with the mucosal immune system. Some bifidobacteria species are immunoregulatory and induce increased dendritic cell interleukin 10 (IL- 10) release in vitro. Fructo-oligosaccharides (FOS) increase faecal and mucosal bifidobacteria in healthy volunteers. The aim of this study was to assess the effect of FOS administration on disease activity, bifidobacteria concentrations, and mucosal dendritic cell function in patients with moderately active Crohn’s disease. Patients and methods: Ten patients with active ileocolonic Crohn’s disease received 15 g of FOS for three weeks. Disease activity was measured using the Harvey Bradshaw index. Faecal and mucosal bifidobacteria were quantified by fluorescence in situ hybridisation, and mucosal dendritic cell IL- 10 and Toll-like receptor (TLR) expression were assessed by flow cytometry of dissociated rectal biopsies. Results: FOS induced a significant reduction in the Harvey Bradshaw index from 9.8 (SD 3.1) to 0.9 (3.4) (p < 0.01). There was a significant increase in faecal bifidobacteria concentration from 8.8 (0.9) log10 to 9.4 (0.9)- log10 cells/g dry faeces (p < 0.001). The percentage of IL- 10 positive dendritic cells increased from 30 (12)% to 53 (10)% (p = 0.06). Finally, the percentage of dendritic cells expressing TLR2 and TLR4 increased from 1.7 (1.7)% to 36.8 (15.9)% (p = 0.08) and from 3.6 (3.6)% to 75.4 (3.4)% (p < 0.001), respectively. Conclusions: FOS supplementation increases faecal bifidobacteria concentrations and modifies mucosal dendritic cell function. This novel therapeutic strategy appears to decrease Crohn’s disease activity in a small open label trial and therefore warrants further investigation. | Lindsay J.O. Whelan K. Stagg A.J. 程妍 | 2006 | 世界核心医学期刊文摘(胃肠病学分册)2006，2，8： | 2 |
| 13 | Dietary Bacillus spp.enhanced growth and disease resistance of weaned pigs by modulating intestinal microbiota and systemic immunity显示文摘Background:Previous research has shown that dietary supplementation of Bacillus spp.probiotics exerts beneficial effects on animals’growth.However,limited studies have evaluated the efficacy of Bacillus spp.on weaned pigs and their effects on host gut health and microbiome,and systemic immunity using a disease challenge model.The objective of this experiment was to investigate the effects of two Bacillus spp.strains(Bacillus subtilis DSM 32540 and Bacillus pumilus DSM 32539)on growth performance,diarrhea,intestinal health,microbiome,and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli(ETEC).Results:Pigs in PRO1(Bacillus subtilis DSM 32540)had greater(P<0.05)body weight on d 7 and 14 PI,greater(P<0.05)ADG from d 0 to 7 and d 7 to 14 PI,compared with pigs in CON(Control).Pigs in PRO1 had milder(P<0.05)diarrhea on d 2 and 3 PI compared with pigs in CON.However,no differences were observed in growth performance and diarrhea score between PRO2(Bacillus pumilus DSM 32539)and CON groups.Supplementation of PRO1 decreased(P<0.05)lymphocyte counts on d 7 and 14 PI,compared with CON.Supplementation of PRO1 and PRO2 both reduced(P<0.05)total coliforms in mesenteric lymph nodes on d 21 PI.Pigs in PRO2 had greater(P<0.05)goblet cell number and sulfomucin percentage in duodenal villi and greater(P<0.05)sialomucin percentage in jejunal villi than pigs in CON.Supplementation of PRO1 up-regulated(P<0.05)MUC2 gene expression in jejunal mucosa and reduced(P<0.05)PTGS-2 and IL1B gene expression in ileal mucosa on d 21 PI,compared with CON.Pigs in PRO1 had reduced(P<0.05)relative abundance of families Lachnospiraceae,Peptostreptococcaceae and Pasteurellaceae in the ileum.Conclusions:Supplementation of Bacillus subtilis DSM 32540 improved growth performance,alleviated diarrhea severity,enhanced gut health,and reduced systemic inflammation of weaned pigs infected with ETEC F18.Although Bacillus pumilus DSM 32539 was able to alleviate systemic inflammation,it had limited impacts on growth performance and severity of diarrhea of ETEC F18 challenged weaned pigs. | Yijie He Cynthia Jinno Kwangwook Kim Zhaohai Wu Bie Tan Xunde Li Rose Whelan Yanhong Liu | 2021 | Journal of Animal Science and Biotechnology2021，12，2： | 2 |
| 14 | Systematic review: faecal microbiota transplantation in the management of inflammatory bowel disease显示文摘 | J. L. Anderson R. J. Edney K. Whelan | 2012 | Aliment Pharmacol Ther2012，，6： | 2 |
| 15 | Intestinal tuberculosis: return of an old disease显示文摘 | Karen D. Horvath Richard L. Whelan | 1998 | The American Journal of Gastroenterology1998，，5： | 2 |
| 16 | Intermittent Androgen Deprivation for Locally Advanced and Metastatic Prostate Cancer: Results from a Randomised Phase 3 Study of the South European Uroncological Group显示文摘 | Fernando E.C. Calais da Silva Aldo V. Bono Peter Whelan Maurizio Brausi Anton Marques Queimadelos Jose A. Portillo Martin Ziya Kirkali Fernando M.V. Calais da Silva Chris Robertson | 2009 | European Urology2009，，6： | 2 |
| 17 | Effective DNA vaccination of cattle with the mycobac terial antigens MPB83 and MPB70 does not compromise the specificity of the comparative intradermal tuberculin skin test显示文摘 | Vordermeier H M Cockle P J Whelan A O | 2000 | Vaccine2000，19，210： | 1 |
| 18 | 40-Gbit/s OEIC on GaAs substrate through metamorphic buffer technology显示文摘 | Zhang Y Whelan C S Leoni R | 2003 | IEEE Electron Device Lett2003，24，9： | 1 |
| 19 | Trocar site recurrence is unlikely to result from aerosolization of tumor cells 显示文摘 | Sellers GJ Alleudorf JD | 1996 | Dis Colon Rectum1996，39，10： | 1 |
| 20 | Gefitinib in second line treatment of metastatic or locally advanced synovial sarcoma expressing HERI:A phase Ⅱ trial of EORTC Soft Tissue and Bone Sarcoma Group显示文摘 | Blay J Le Cesne A Whelan J | 2006 | J Clin Oncol2006，24，： | 1 |
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