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| 1 | Antioxidant and immunomodulatory activities of a polysaccharide from Flammulina velutipes显示文摘OBJECTIVE: To evaluate the antioxidant and immunomodulatory activities of a unique polysaccharide from the medicinal fungus Flammulina velutipes in vitro.METHODS: Using water extraction and alcohol precipitation, crude polysaccharides were obtained. After purification by DEAE-cellulose 52 ion exchange chromatography and Sephacryl S-300 HR gel filtration chromatography, High performance liquid chromatography equipped with evaporative light-scattering detector, Infrared radiation and Nuclear magnetic resonance were used to evaluate the structure of the polysaccharide. Its immunomodulatory activity was measured by examining the production of nitric oxide(NO) and cytokine secretion, and via lymphocyte proliferation experiments. Its effects on the scavenging activities of hydroxyl radical, superoxide anion and reducing power were also measured.RESULTS: A water-soluble polysaccharide, Flammulina velutipes polysaccharide I-A(FVP I-A), was obtained with a molecular mass of 8.14×104Da determined by high performance gel permeation chromatography. An in vitro antioxidant assay indicated that FVP I-A could scavenge hydroxyl radical, superoxide anion and possessed reducing power and could largely promote NO production and augment the interleukin-1β, interleukin-6, and tumor necrosis factor-α secretion by RAW264.7 macrophages(P<0.05). Moreover, FVP I-A could promote lymphocyte proliferation(P<0.05), and synergistically enhance the augmentation of the proliferation of mouse lymphocytes by concanavalin A and lipopolysaccharides(P<0.01, P<0.05).CONCLUSION: The FVP I-A obtained from Flammulina velutipes possessed antioxidant activity and could enhance non-specific and specific immune responses in vitro. | Ming Wu Xia Luo Xiaoyan Xu Wei Wei Mengyao Yu Nan Jiang Liming Ye Zhirong Yang Xiaofan Fei | 2014 | Journal of Traditional Chinese Medicine2014，34，6： | 14 |
| 2 | Metal distribution characteristic of MSWI bottom ash in view of metal recovery显示文摘Bottom ash is the major by-product of municipal solid waste incineration(MSWI), and is often reused as an engineering material, such as road-base aggregate. However, some metals(especially aluminum) in bottom ash can react with water and generate gas that could cause expansion and failure of products containing the ash; these metals must be removed before the ash is utilized. The size distribution and the chemical speciation of metals in the bottom ash from two Chinese MSWI plants were examined in this study, and the recovery potential of metals from the ash was evaluated. The metal concentrations in these bottom ashes were lower than that generated in other developed countries. Specifically, the contents of Al,Fe, Cu and Zn were 18.9–29.2, 25.5–32.3, 0.7–1.0 and 1.6–2.5 g/kg, respectively. Moreover,44.9–57.0 wt.% of Al and 55.6–75.4 wt.% of Fe were distributed in bottom ash particles smaller than 5 mm. Similarly, 46.6–79.7 wt.% of Cu and 42.9–74.2 wt.% of Zn were concentrated in particles smaller than 3 mm. The Fe in the bottom ash mainly existed as hematite, and its chemical speciation was considered to limit the recovery efficiency of magnetic separation. | Yi Xia Pinjing He Liming Shao Hua Zhang | 2017 | Journal of Environmental Sciences2017，29，2： | 11 |
| 3 | Developmental changes in intercellular junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods显示文摘Background: The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different intercellular junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, intercellular junctions and voltage-gated potassium(Kv) channels in the intestine of piglets during the suckling and post-weaning periods.Results: Samples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen(PCNA)-positive cells and alkaline phosphatase(AKP) activity, as well as the abundances of E-cadherin,occludin, and Kv1.5 m RNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1to d 21 during the suckling period(P < 0.05). Weaning induced decreases in the percentage of PCNA-positive cells,AKP activity and the abundances of E-cadherin, occludin and zonula occludens(ZO)-1 m RNA or protein in the jejunum on d 1, 3 and 5 post-weaning(P < 0.05). There were lower abundances of E-cadherin, occludin and ZO-1m RNA as well as claudin-1, claudin-3 and ZO-1 protein in the jejunum of weanling piglets than in 21-d-old suckling piglets(P < 0.05). The abundances of E-cadherin, occludin, ZO-1 and integrin m RNA were positively related to the percentage of PCNA-positive cells.Conclusion: Weaning at 14 d of age induced damage to the intestinal morphology and barrier. While there was an adaptive restoration on d 7 post-weaning, the measured values did not return to the pre-weaning levels, which reflected the impairment of intercellular junctions and Kv channels. | Jing Wang Liming Zeng Bie Tan Guangran Li Bo Huang Xia Xiong Fengna Li Xiangfeng Kong Gang Liu Yulong Yin | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 8 |
| 4 | Leaching toxicity characteristics of municipal solid waste incineration bottom ash显示文摘市政的稳固的浪费焚化底部灰(MSWIBA ) 的连续地增加的生产作为构造材料支持了它的利用并且提起了环境担心。物理化学的性质和 MSWIBA 的沥滤的行为被学习,并且用光细菌生物鉴定测试的 ecotoxicological 被执行估计与它的沥滤的成分联系的生态的污染风险。MSWIBA 被 leachants 的二种类型沥滤, H 2 那么 4/HNO3 和 HAc 答案,在到稳固的比率和接触时间的不同液体。在 leachates 的重金属和阴离子的集中被分析。包括原则部件分析,皮尔森关联分析和层次簇分析, Multivariate 统计分析被用来评估成分的贡献到毒性(MSWIBA leachate 的 EC 50) 。ecotoxicological 结果的统计分析证明 Ba, Cr, Cu, Pb, F 并且全部的器官的碳(TOC ) 集中密切与 EC 50 价值被相关,并且这些物质是到 MSWIBA leachate 的 ecotoxicity 的主要贡献者。另外,这些变量的簇显示了类似的沥滤的行为。总的来说,研究证明源于沥滤的 MSWIBA 的 ecotoxicological 风险能在它的利用前被估计,它为 MSWIBA 同样其他的材料的改编提供关键信息。 | Khamphe PHOUNGTHONG Yi XIA Hua ZHANG Liming SHAO Pinjing HE | 2016 | Frontiers of Environmental Science & Engineering2016，10，2： | 5 |
| 5 | Size-tunable synthesis of gold nanorods using pyrogallol as a reducing agent显示文摘A novel and facile seed-mediated method for the preparation of monodispersed gold nanorods(GNRs) is presented by introducing pyrogallol as a reductant. Fast Fourier transformation of high-resolution transmission electron microscopy reveals that the synthesized GNRs are single crystalline. The longitudinal surface plasmon resonance of GNRs can be finely tuned by varying silver ion concentrations or seed amounts. Also, both thick(diameter >30 nm) and thin(diameter <10 nm) GNRs with exceptional monodispersity can be well prepared by this method. These findings indicate that this method has a greater performance in controlling the morphology of GNRs than that of traditional approach with ascorbic acid as a reductant. | Yuanfu Huang Kai Xia Nongyue He Zhuoxuan Lu Liming Zhang Yan Deng Libo Nie | 2015 | Science China Chemistry2015，58，11： | 3 |
| 6 | Cardiac Na^(+)-Ca^(2+) exchanger 1(ncx1h)is critical for the ventricular cardiomyocyte formation via regulating the expression levels of gata4 and hand2 in zebrafish显示文摘Ca^(2+) signaling is critical for heart development;however,the precise roles and regulatory pathways of Ca^(2+) transport proteins in cardiogenesis remain largely unknown.Sodium-calcium exchanger 1(Ncx1)is responsible for Ca^(2+) efflux in cardiomyocytes.It is involved in cardiogenesis,while the mechanism is unclear.Here,using the forward genetic screening in zebrafish,we identified a novel mutation at a highly-conserved leucine residue in ncx1 gene(mutant^(LDD353)/ncx1h^(L154P))that led to smaller hearts with reduced heart rate and weak contraction.Mechanistically,the number of ventricular but not atrial cardiomyocytes was reduced in ncx1h^(L154P) zebrafish.These defects were mimicked by knockdown or knockout of ncx1h.Moreover,ncx1h^(L154P) had cytosolic and mitochondrial Ca^(2+) overloading and Ca^(2+) transient suppression in cardiomyocytes.Furthermore,ncx1h^(L154P) and ncx1h morphants downregulated cardiac transcription factors hand2 and gata4 in the cardiac regions,while overexpression of hand2 and gata4 partially rescued cardiac defects including the number of ventricular myocytes.These findings demonstrate an essential role of the novel 154th leucine residue in the maintenance of Ncx1 function in zebrafish,and reveal previous unrecognized critical roles of the 154th leucine residue and Ncx1 in the formation of ventricular cardiomyocytes by at least partially regulating the expression levels of gata4 and hand2. | Liming Chu Huimin Yin Lei Gao Li Gao Yu Xia Chiyuan Zhang Yi Chen Tingxi Liu Jijun Huang Kenneth R.Boheler Yong Zhou Huang-Tian Yang | 2021 | Science China(Life Sciences)2021，64，2： | 3 |
| 7 | Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate 显示文摘 | Chen Ming Xia Liming Xue Peijian | 2007 | International Biodeterioration and Biodegradation2007，59，： | 1 |
| 8 | Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation显示文摘 | Renhua Huang Renxue Xia Liming Hu Yunmei Lu Mingyuan Wang | 2007 | Scientia Horticulturae2007，，2： | 1 |
| 9 | Effects of Transdermal Estrogen Therapy on Expressions of Estrogen Receptors and T-lymphocyte Apoptosis in Surgically Menopausal Women显示文摘ng。 | Jing Zhang Xing Chen Shaofen Zhang Guangyan Zhou Xian Xia Liming Lu | 2009 | Cellular & Molecular Immunology2009，6，4： | 1 |
| 10 | Influence of steam explosion pretreatment on plant fibre显示文摘 | Hen Yuru Xia Liming | 1999 | Journal of chemical engineering of Chinese universities1999，6，13： | 1 |
| 11 | Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation显示文摘 | HUANG Renhua XIA Renxue HU Liming | | 0，，： | 1 |
| 12 | High-yield cellulase production by Trichoderma reesei ZU-02 on corn cob residue显示文摘 | XIA LIMING SHEN XUELIANG | 2004 | Bioresource Technology2004，91，3： | 1 |
| 13 | Arabidopsis EXTRA-LARGE G PROTEIN 1(XLG1) functions together with XLG2 and XLG3 in PAMP-triggered MAPK activation and immunity显示文摘Pattern-triggered immunity(PTI) is an essential strategy used by plants to deploy broad-spectrum resistance against pathogen attacks. Heterotrimeric G proteins have been reported to contribute to PTI.Of the three non-canonical EXTRA-LARGE G PROTEINs(XLGs) in Arabidopsis thaliana, XLG2 and XLG3 were shown to positively regulate immunity,but XLG1 was not considered to function in defense,based on the analysis of a weak xlg1 allele.In this study, we characterized the xlg1 xlg2 xlg3 triple knockout mutants generated from an xlg1 knockout allele. The strong xlg1 xlg2 xlg3 triple mutants compromised pathogen-associated molecular pattern(PAMP)-triggered activation of mitogen-activated protein kinases(MAPKs) and resistance to pathogen infection. The three XLGs interacted with MAPK cascade proteins involved in defense signaling, including the MAPK kinase kinases MAPKKK3 and MAPKKK5, the MAPK kinases MKK4 and MKK5, and the MAPKs MPK3 and MPK6. Expressing a constitutively active form of MKK4 restored MAPK activation and partially recovered the compromised disease resistance seen in the strong xlg1 xlg2 xlg3 triple mutant. Furthermore, mutations of all three XLGs largely restored the phenotype of the autoimmunity mutant bak1-interacting receptor-like kinase 1. Our study reveals that all three XLGs function redundantly in PAMP-triggered MAPK activation and plant immunity. | Yiping Wang Hailei Zhang Pengxi Wang Huan Zhong Wuzhen Liu Shoudong Zhang Liming Xiong Yingying Wu Yiji Xia | 2023 | Journal of Integrative Plant Biology2023，65，3： | 1 |
| 14 | Enzymatic hydrolysis of comcob and ethanol production from cellulosic hydrolysate显示文摘 | Ming Chen Liming Xia Peijian Xue | 2007 | International Biodeterioration & Biodegradation2007，59，： | 1 |
| 15 | Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry显示文摘 | Xia Liming Cen Peilin | 1999 | Process Biochemistry1999，34，9： | 1 |
| 16 | Production and immobilization of cellobiase from Aspergillus niger ZU-07显示文摘 | Xueliang Shen Liming Xia | 2004 | Process Biochemistry2004，39，： | 1 |
| 17 | Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate显示文摘 | Chen Ming Xia Liming Xue Peijian | 2007 | International Biodeterilration and Biodegradation2007，59，： | 1 |
| 18 | Enhanced bioproduction of chitin in engineered Pichia pastoris显示文摘Biopolymer chitin and its derivatives have a wide range of applications in different fields.Chitin is primarily produced from marine crustacean by strong acid and strong alkali treatment.To reduce the environmental hazardous in the production of chitin,post-fermentation fungal biomass waste like fungi cell walls provides a viable alternative source for chitin.In this study,improved chitin content in yeast cell wall was achieved by overexpressing chitin biosyntheic pathway related genes.The chitin content was 51.5μg/mg cell wall and 122.9μg/mg cell wall in strain GS-1.10(gfat,uap and chs1 genes overexpression)and strain GS-2.6(gfat,gna1,agm1,uap and chs1 genes overexpression),which were 44.3%and 244.4%higher than that in control strain GS115,respectively.By overexpressing another chitin synthase gene(chs3),the chitin content in strain GS-3.10 was further increased to 136.2μg/mg cell wall,which was 10.8%and 281.6%higher than that in GS-2.6 and in control GS115,respectively.Moreover,chitin yield was further improved by various culture conditions optimization,and reached 162.4μg/mg cell wall,which was 4.43 times of that in the starting strain GS115.The final titer of chitin was 2.23 g/L culture broth in 84 h fed-batch fermentation in a 5 L bioreactor.To our knowledge,this is the first report of chitin production in engineered Pichia pastoris via biosynthetic pathway enhancement. | Xue Zhang Chunyue Zhang Mian Zhou Quanming Xia Liqiang Fan Liming Zhao | 2022 | Food Bioscience2022，47，3： | 1 |
| 19 | Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry 显示文摘 | Xia Liming Cen Peilin | 1999 | Process Biochemistry1999，34，： | 1 |
| 20 | Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry显示文摘 | Xia Liming Cen Peilin | 1999 | Process biochemistry1999，34，9： | 1 |
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