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| 1 | Thinking on the informatization development of China’s healthcare system in the post-COVID-19 era显示文摘With the application of Internet of Things,big data,cloud computing,artificial intelligence,and other cuttingedge technologies,China’s medical informatization is developing rapidly.In this paper,we summaried the role of information technology in healthcare sector’s battle against the coronavirus disease 2019(COVID-19)from the perspectives of early warning and monitoring,screening and diagnosis,medical treatment and scientific research,analyzes the bottlenecks of the development of information technology in the post-COVID-19 era,and puts forward feasible suggestions for further promoting the construction of medical informatization from the perspectives of sharing,convenience,and safety. | Ming Zhang Danyun Dai Siliang Hou Wei Liu Feng Gao Dong Xu Yu Hu | 2021 | Intelligent Medicine2021，1，1： | 4 |
| 2 | Aldehyde dehydrogenase ALDH3F1 involvement in flowering time regulation through histone acetylation modulation on FLOWERING LOCUS C显示文摘Flowering time regulation is one of the most important processes in the whole life of flowering plants and FLOWERING LOCUS C(FLC)is a central repressor of flowering time.However,whether metabolic acetate level affects flowering time is unknown.Here we report that ALDEHYDE DEHYDROGENASE ALDH3F1 plays essential roles in floral transition via FLC-dependent pathway.In the aldh3f1-1 mutant,the flowering time was significant earlier than Col-0 and the FLC expression level was reduced.ALDH3F1 had aldehyde dehydrogenase activity to affect the acetate level in plants,and the amino acids of E214 and C252 are essential for its catalytic activity.Moreover,aldh3f1 mutation reduced acetate level and the total acetylation on histone H3.The H3K9Ac level on FLC locus was decreased in aldh3f1-1,which reduced FLC expression.Expression of ALDH3F1 could rescue the decreased H3K9Ac level on FLC,FLC expression and also the early-flowering phenotype of aldh3f1-1,however ALDH3F1E214A or ALDH3F1C252A could not.Our findings demonstrate that ALDH3F1 participates in flowering time regulation through modulating the supply of acetate for acetyl-CoA,which functions as histone acetylation donor to modulate H3K9Ac on FLC locus. | Danyun Xu Qing Liu Gang Chen Zhiqiang Yan Honghong Hu | 2020 | Journal of Integrative Plant Biology2020，62，8： | 2 |
| 3 | NbSe2 nanosheet supported PbBiO2Br as a high performance photocatalyst for the visible light-driven asymmetric alkylation of aldehyde显示文摘 | LI Xiaojie WANG Jun XU Danyun | 2015 | Sustainable Chem Eng2015，3，5： | 1 |
| 4 | Preferences in Wikipedia abstracts: Empirical findings and implications for automatic entity summarization显示文摘 | Xu Danyun Cheng Gong Qu Yunzhong | 2014 | Information Processing & Management2014，50，2： | 1 |
| 5 | The binding epitope of sintilimab on PD-1 revealed by AbMap显示文摘PD-1 plays an important role as an immune checkpoint.Sintilimab is a newly approved PD-1 antibody for cancer immunotherapy with an unknown binding epitope on PD-1.In this study,to elucidate the molecular mechanism by which sintilimab blocks PD-1 activation,we applied Antibody binding epitope Mapping(AbMap)to identify the binding epitope of sintilimab.An epitope was successfully identified,i.e.SLAPKA,aa 127-132.By constructing a series of point mutations,the dominant residues S127,L128,A129,P130,and A132 of PD-1 were further validated by western blot analysis,biolayer interferometry,and flow cytometry.Structural analysis showed that the epitope is partially within the binding interface of PD-1 and PD-L1,and this epitope also partially overlaps with that of nivolumab and pembrolizumab.These results demonstrate that sintilimab can attenuate PD-1 activation by directly competing with the interaction between PD-1 and PD-L1 through binding with the key residues of the FG loop on PD-1.This study also demonstrates the high efficiency and accuracy of AbMap for determining the binding epitope of therapeutic antibodies. | Mingliang Ma Huan Qi Chuansheng Hu Zhaowei Xu Fanlin Wu Nan Wang Danyun Lai Yang Li Hainan Zhang Hewei Jiang Qingfeng Meng Shujuan Guo Yani Kang Xiaodong Zhao Hua Li Sheng-ce Tao | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 1 |
| 6 | Regulation of FLC nuclear import by coordinated action of the NUP62-subcomplex and importinβSAD2显示文摘Flowering locus C(FLC)is a central transcriptional repressor that controls flowering time.However,how FLC is imported into the nucleus is unknown.Here,we report that Arabidopsis nucleoporins 62(NUP62),NUP58,and NUP54 composed NUP62-subcomplex modulates FLC nuclear import during floral transition in an importinα-independent manner,via direct interaction.NUP62 recruits FLC to the cytoplasmic filaments and imports it into the nucleus through the NUP62-subcomplex composed central channel.Importinβsupersensitive to ABA and drought 2(SAD2),a carrier protein,is critical for FLC nuclear import and flower transition,which facilitates FLC import into the nucleus mainly through the NUP62-subcomplex.Proteomics,RNAseq,and cell biological analyses indicate that the NUP62-subcomplex mainly mediates the nuclear import of cargos with unconventional nuclear localization sequences(NLSs),such as FLC.Our findings illustrate the mechanisms of the NUP62-subcomplex and SAD2 on FLC nuclear import process and floral transition,and provide insights into the role of NUP62-subcomplex and SAD2 in protein nucleocytoplasmic transport in plants. | Gang Chen Danyun Xu Qing Liu Zhichuang Yue Biao Dai Shujuan Pan Yongqiang Chen Xinhua Feng Honghong Hu | 2023 | Journal of Integrative Plant Biology2023，65，9： | 0 |
| 7 | De novo transcriptome assembly and metabolomic analysis of three tissue types in Cinnamomum cassia显示文摘Objective:The barks,leaves,and branches of Cinnamomum cassia have been historically used as a traditional Chinese medicine,spice,and food preservative,in which phenylpropanoids are responsible compounds.However phenylpropanoid biosynthesis pathways are not clear in C.cassia.We elucidated the pathways by descriptive analyses of differentially expressed genes related to phenylpropanoid biosynthesis as well as to identify various phenylpropanoid metabolites.Methods:Chemical analysis,metabolome sequencing,and transcriptome sequencing were performed to investigate the molecular mechanisms underlying the difference of active components content in the barks,branches and leaves of C.cassia.Results:Metabolomic analysis revealed that small amounts of flavonoids,coumarine,and cinnamaldehyde accumulated in both leaves and branches.Transcriptome analysis showed that genes associated with phenylpropanoid and flavonoid biosynthesis were downregulated in the leaves and branches relative to the barks.The observed differences in essential oil content among the three tissues may be attributable to the differential expression of genes involved in the phenylpropanoid and flavonoid metabolic pathways.Conclusion:This study identified the key genes in the phenylpropanoid pathway controling the flavonoid,coumarine,and cinnamaldehyde contents in the barks,branches and leaves by comparing the transcriptome and metabolome.These findings may be valuable in assessing phenylpropanoid and flavonoid metabolites and identifying specific candidate genes that are related to the synthesis of phenylpropanoids and flavonoids in C.cassia. | Hongyang Gao Huiju Zhang Yuqing Hu Danyun Xu Sikai Zheng Shuting Su Quan Yang | 2023 | Chinese Herbal Medicines2023，15，2： | 0 |
| 8 | IQ67 DOMAIN protein 21 is critical for indentation formation in pavement cell morphogenesis显示文摘In plants, cortical microtubules anchor to the plasma membrane in arrays and play important roles in cell shape. However, the molecular mechanism of microtubule binding proteins, which connect the plasma membrane and cortical microtubules in cell morphology remains largely unknown. Here, we report that a plasma membrane and microtubule duallocalized IQ67 domain protein, IQD21, is critical for cotyledon pavement cell(PC) morphogenesis in Arabidopsis. iqd21 mutation caused increased indentation width, decreased lobe length, and similar lobe number of PCs, whereas IQD21 overexpression had a different effect on cotyledon PC shape. Weak overexpression led to increased lobe number, decreased indentation width, and similar lobe length, while moderate or great overexpression resulted in decreased lobe number, indentation width, and lobe length of PCs. Live-cell observations revealed that IQD21 accumulation at indentation regions correlates with lobe initiation and outgrowth during PC development. Cell biological and genetic approaches revealed that IQD21 promotes transfacial microtubules anchoring to the plasma membrane via its polybasic sites and bundling at the indentation regions in both periclinal and anticlinal walls. IQD21 controls cortical microtubule organization mainly through promoting Katanin 1-mediated microtubule severing during PC interdigitation. These findings provide the genetic evidence that transfacial microtubule arrays play a determinant role in lobe formation, and the insight into the molecular mechanism of IQD21 in transfacial microtubule organization at indentations and puzzle-shaped PC development. | Xinhua Feng Shujuan Pan Haifu Tu Junjie Huang Chuanlei Xiao Xin Shen Lei You Xinyan Zhao Yongqiang Chen Danyun Xu Xiaolu Qu Honghong Hu | 2023 | Journal of Integrative Plant Biology2023，65，3： | 0 |
| 9 | SARS-CoV-2-specific antibody response characteristics in COVID-19 patients of different ages显示文摘Age has been found to be one of the main risk factors for the severity and outcome of COVID-19.However,differences in SARS-CoV-2 specific antibody responses among COVID-19 patients of different age groups remain largely unknown.In this study,we analyzed the IgG/IgM responses to 21 SARS-CoV-2 proteins and 197 peptides that fully cover the spike protein against 731 sera collected from 731 COVID-19 patients aged from 1 to 92 years.We show that there is no overall difference in SARS-CoV-2 antibody responses in COVID-19 patients in the 4 age groups.By antibody response landscape maps,we find that the IgG response profiles of SARS-CoV-2 proteins are positively correlated with age.The S protein linear epitope map shows that the immunogenicity of the S-protein peptides is related to peptide sequence,disease severity and age of the COVID-19 patients.Furthermore,the enrichment analysis indicates that low S1 IgG responses are enriched in patients aged<50 and high S1 IgG responses are enriched in mild COVID-19 patients aged>60.In addition,high responses of non-structural/accessory proteins are enriched in severe COVID-19 patients aged>70.These results suggest the distinct immune response of IgG/IgM to each SARS-CoV-2 protein in patients of different age,which may facilitate a deeper understanding of the immune responses in COVID-19 patients. | Linfang Lu Siqi Yu Min Liu Yang Li Qing Lei Mingxi Lin Danyun Lai Shujuan Guo Hewei Jiang Hongyan Hou Yunxiao Zheng Xuening Wang Mingliang Ma Bo Zhang Hong Chen Junbiao Xue Hainan Zhang Huan Qi Ziyong Sun Feng Wang Xionglin Fan Zhaowei Xu | 2022 | Acta Biochimica et Biophysica Sinica2022，54，4： | 0 |
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