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| 1 | JMJD3 is a histone H3K27 demethylase显示文摘组蛋白甲基化是参予细胞的过程的一个多样的数组的重要 epigenetic 现象并且被发现了与癌症被联系。几的 Recentidentification 嘘一 demethylases 证明了那嘘一甲基化是一个可逆过程。通过一条候选人途径,我们化学上有简历作为 H3K27demethylase 识别了 JMJD3。进 HeLa 房间的 JMJD3 的 Transfection 引起了整齐的乙醇 H3K27 的特定的减小,但是没在 di- 和单音的甲基 H3K27 上有效果,或嘘 H3K4 和 H3K9 上的一离氨酸 methylations。Theenzymatic 活动要求 JmjC 域和被建议了为余因子绑定重要的保存组氨酸。试管内生物化学的实验证明那 JMJD3directly 催化 demethylation。另外,我们发现 JMJD3 起来在前列腺癌症,和它的表示调整了在变形前列腺癌症是更高的。因此,我们作为能够把整齐的乙醇组从移开的 ademethylase 识别了 JMJD3 嘘一 H3 离氨酸 27 并且起来在前列腺癌症调整了。 | Yang Xiang Ziqi Zhu Gang Han Hanqing Lin Longyong Xu Charlie Degui Chen | 2007 | Cell Research2007，17，10： | 37 |
| 2 | Quantitative determination of AI-2 quorum-sensing signal of bacteria using high performance liquid chromatography–tandem mass spectrometry显示文摘Autoinducer 2(AI-2), an important bioactive by-product of the Lux S-catalyzed S-ribosylhomocysteine cleavage reaction in the activated-methyl-cycle, has been suggested to serve as a universal intra- and inter-species signaling molecule. The development of reliable and sensitive methods for quantitative determination of AI-2 is highly desired.However, the chemical properties of AI-2 cause difficulty in its quantitative analysis.Herein, we report a high performance liquid chromatography-tandem mass spectrometric method that enables reproducible and sensitive measurement of AI-2 concentrations in complex matrixes. 4,5-Dimethylbenzene-1,2-diamine(DMBDM), an easy-to-obtain commercial reagent, was used for the derivatization treatment. The assay was linear in the concentration range of 1.0–1000 ng/m L(R^2= 0.999) and had a lower limit of quantification of0.58 ng/m L. The method exhibited several advantages, e.g., high selectivity, wide linear response range, and good sensitivity. Furthermore, the effectiveness of the method was further validated through measuring AI-2 concentrations in the cell-free culture supernatant from Escherichia coli wild type. | Fang Xu Xiangning Song Peijie Cai Guoping Sheng Hanqing Yu | 2017 | Journal of Environmental Sciences2017，29，2： | 8 |
| 3 | Development and characterization of a clinical strain of Coxsackievirus A16 and an eGFP infectious clone显示文摘Coxsackievirus A16(CA16) is one of the major causes of hand, foot, and mouth disease(HFMD) worldwide, which is a common illness that affects children. The frequent occurrence of HFMD outbreaks has become a serious public health problem in Asia. Therefore, it is important to understand the pathogenesis and replication of CA16. In this study, a stable infectious c DNA clone of an epidemic strain of Coxsackievirus A16(CA16) was assembled, and subsequently a reporter virus(e GFP-CA16) was constructed by inserting the e GFP gene between the 5'-UTR and the N-terminus of VP4, with the addition of a 2A protease cleavage site(ITTLG) at its C-terminus. This was transfected into Vero cells to generate infectious recombinant viruses. The growth characteristics and plaque morphology, in vitro, in mammalian cells were found to be indistinguishable between the parental and recombinant viruses. Although the e GFP-CA16 showed smaller plaque size as compared to recombinant CA16, both were found to exhibit similar growth trends and EC50 of NITD008. In summary, this stable infectious c DNA clone should provide a valuable experimental system to study CA16 infection and host response. The e GFP-CA16 is expected to provide a powerful tool to monitor e GFP expression in infected cells and to evaluate the antiviral activity of potential antiviral agents in the treatment of CA16 infections. | Chenglin Deng Xiaodan Li Siqing Liu Linlin Xu Hanqing Ye Cheng-Feng Qin Bo Zhang | 2015 | Virologica Sinica2015，30，4： | 5 |
| 4 | Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘Histone 离氨酸 methylation 能被 JmjC 包含域的蛋白质在 sequence-state-specific 和 methylation-state-specific 举止移开。然而,底层特性怎么样,决定,酶怎么被调整,大部分是未知的。我们最近发现了那 ceKDM7A,一个哲学博士 -- 并且 JmjC 包含域的蛋白质,是为 H3K9me2 特定的 histone demethylase, H3K27me2,和 PHD 摸绑定到 H3K4me3 指南在 vivo 的 demethylation 活动。为了为酶的活动和 PHD 的功能提供结构的卓见进分子的机制,摸,我们与包含 H3K4me3, H3K9me2,和 H3K27me2 修正的各种各样的联合的单身者或二肽一起在 apo 形式并且在建筑群解决了酶的六水晶结构。结构显示 H3K9me2 和 H3K27me2 以一种类似的方式与 ceKDM7A 交往,并且肽绑定特性被特定的相互作用的一个网络决定。结构的几何测量也揭示了与 PHD 手指和 H3K9me2 界限联系到 JmjC 领域的那 H3K4me3 从二个分开的分子,建议 trans-histone 肽绑定机制。因此,我们的全身的结构的研究重要地由催化领域而且更多揭示底层识别不仅,为 H3K9me2 和 H3K27me2 的 ceDKM7A 的双特性的分子的机制。 | Ying Yang Lulu Hu Ping Wang Haifeng Hou Yan Lin Yi Liu Ze Li Rui Gong Xiang Feng Lu Zhou Wen Zhang Yuhui Dong Huirong Yang Hanqing Lin Yiqin Wang Charlie Degui Chen Yanhui Xu | 2010 | Cell Research2010，20，8： | 3 |
| 5 | Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘H3K9me2 和 H3K27me2 是与抄写压抑联系的重要 epigenetic 标记,当 H3K4me3 与抄写激活被联系时。活跃、压抑的 histone methylations 以一种互相独占的方式散布,这被显示出,但是内在的机制糟糕被理解。这里,我们识别了 ceKDM7A,一个哲学博士(植物 homeodomain )- 并且 JmjC 包含域的蛋白质,为 H3K9me2 和 H3K27me2 特定的 histone demethylase。我们进一步证明 ceKDM7A 的 PHD 领域在染色体宽的水平绑了 H3K4me3 和与 ceKDM7A co 局部性的 H3K4me3。到 H3K4me3 的 PHD 域绑定的混乱在 vivo 减少了 demethylase 活动,并且 ceKDM7A 的损失减少了它的联系目标基因的表达式。这些结果显示 ceKDM7A 被招募到倡导者到 demethylate H3K9me2 和 H3K27me2 并且通过 PHD 领域的绑定激活基因表示到 H3K4me3。因此,我们的学习识别双特性的 histone demethylase 并且提供新奇卓见进 histone methylation 的规定。 | Hanqing Lin Yiqin Wang Yanru Wang Feng Tian Pu Pu Yi Yu Hailei Mao Ying Yang Ping Wang Lulu Hu Yan Lin Yi Liu Yanhui Xu Charlie Degui Chen | 2010 | Cell Research2010，20，8： | 2 |
| 6 | Determination of metformin in diabetic rat plasma by an improved ion-pair high- performance liquid chromatography: application to a pharma- cokineticstudy显示文摘 | Chen Ye Li HanQing Xu JiaoJiao et aL | 2012 | J ChinPharmSci2012，21，3： | 1 |
| 7 | Environmental pollution,a hidden culprit for health issues显示文摘The environmental and health impacts from the massive discharge of chemicals and subsequent pollution have been gaining increasing public concern.The unintended exposure to different pollutants,such as heavy metals,air pollutants and organic chemicals,may cause diverse deleterious effects on human bodies,resulting in the incidence and progression of different diseases.The article reviewed the outbreak of environmental pollution-related public health emergencies,the epidemiological evidence on certain pollution-correlated health effects,and the pathological studies on specific pollutant exposure.By recalling the notable historical life-threatening disasters incurred by local chemical pollution,the damning evidence was presented to criminate certain pollutants as the main culprit for the given health issues.The epidemiological data on the prevalence of some common diseases revealed a variety of environmental pollutants to blame,such as endocrine-disrupting chemicals(EDCs),fine particulate matters(PMs)and heavy metals.The retrospection of toxicological studies provided illustrative clues for evaluating ambient pollutant-induced health risks.Overall,environmental pollution,as the hidden culprit,should answer for the increasing public health burden,and more efforts are highly encouraged to strive to explore the cause-and-effect relationships through extensive epidemiological and pathological studies. | Hanqing Xu Yang Jia Zhendong Sun Jiahui Su Qian S.Liu Qunfang Zhou Guibin Jiang | 2022 | Eco-Environment & Health2022，1，1： | 1 |
| 8 | The preliminary research of the tektite composition in Lei-Qiong region显示文摘 | XU Hanqing HU Guohui ZHONG Honghai | 1983 | Geochemistry1983，3，： | 1 |
| 9 | Role of thioredox- in in lung disease 显示文摘 | Jian Xu Taoping Li Hanqing Wu | 2012 | Pulmonary Pharmacology and Thera- peutics2012，25，2： | 1 |
| 10 | A Novel Framework for Semantic Annotation and Personalized Retrieval of Sports Video 显示文摘 | Xu Changsheng Wang Jinjun Lu Hanqing | 2008 | IEEE Transactions on Muhimediea2008，10，3： | 1 |
| 11 | Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase显示文摘We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV. | Chao Shan Xiaodan Li Chenglin Deng Baodi Shang Linlin Xu Hanqing Ye Zhiming Yuan Bo Zhang | 2013 | Virologica Sinica2013，28，3： | 1 |
| 12 | Research on the Contents of Chinese Seabucklthorn Fruit Oil and Its Application to Chloasma Treatments显示文摘 | Hanqing Xu Junfeng Ren Huili Xu Kefeng Sun Ailin Lv Jie Fen | 2005 | Journal of US-China Medical Science2005，2，6： | 1 |
| 13 | Aging-elevated inflammation promotes DNMT3A R878H-driven clonal hematopoiesis显示文摘Aging-elevated DNMT3A R882H-driven clonal hematopoiesis(CH)is a risk factor for myeloid malignancies remission and overall survival.Although some studies were conducted to investigate this phenomenon,the exact mechanism is still under debate.In this study,we observed that DNMT3 A R878 H bone marrow cells(human allele:DNMT3 A R882 H)displayed enhanced reconstitution capacity in aged bone marrow milieu and upon inflammatory insult.DNMT3 A R878 H protects hematopoietic stem and progenitor cells from the damage induced by chronic inflammation,especially TNFa insults.Mechanistically,we identified that RIPK1-RIPK3-MLKL-mediated necroptosis signaling was compromised in R878 H cells in response to proliferation stress and TNFa insults.Briefly,we elucidated the molecular mechanism driving DNMT3 A R878 H-based clonal hematopoiesis,which raises clinical value for treating DNMT3 A R882 H-driven clonal hematopoiesis and myeloid malignancies with aging. | Min Liao Ruiqing Chen Yang Yang Hanqing He Liqian Xu Yuxuan Jiang Zhenxing Guo Wei He Hong Jiang Jianwei Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 1 |
| 14 | H-Shaped Resonant Optical Antennas with Slot Coupling显示文摘 | Xu Han Xiang Ji Hanqing Wen | 2012 | Plamonics2012，7，： | 1 |
| 15 | Comparison between inhibitor and uncoupler for minimizing excess sludge production of an activated sludge process显示文摘In order to study the minimization of excess sludge production,the reduction in the excess sludge production in the presence of an inhibitor and uncoupler was studied in this work.The experimental results show that such an addition could effectively reduce the production of excess sludge.With the energy uncoupling model established in this work,energy uncoupling coefficient(Eu)was used to evaluate the reduction in excess sludge production.The energy uncoupling coefficients in the presence of dinitrophenol(dNP),Zn^(2+),and Cu^(2+)was 0.75,0.46,and 0.18,respectively.The analysis demonstrated that energy spilling occurred in the presence of dNP,and that dNP was an effective uncoupler for reducing the production of excess activated sludge without affecting the microbial respiration activity. | CHEN Guowei XI Pengge XU Deqian YU Hanqing | 2007 | Frontiers of Environmental Science & Engineering2007，1，1： | 1 |
| 16 | An active bacterial anti-adhesion strategy based on directional transportation of bacterial droplets driven by triboelectric nanogenerators显示文摘An active bacterial anti-adhesion strategy based on directional transportation of bacterial droplets driven by a triboelectric nanogenerator(TENG)has not been reported to date,although passive defense approaches can prevent bacterial adhesion by regulating superwetting surfaces combined with incorporated antibacterial substances.Here a triboelectric nanogenerator driving droplet system(TNDDS)was built to drive directional transportation of bacterial droplets to be eliminated,which comprises TENG with periodical frictional Kapton film and aluminum foils and a superhydrophobic driving platform(SDP)with paralleled driving electrodes.The current generated by the TENG triboelectricity is transmitted to the paralleled driving electrodes to form an electric field driving the directional transportation of charged droplets.The critical value of the driven droplet volume on SDP is closely related to the distributed electrodes’distance and width,and the driving distance of droplets is related to the number of electrodes.More crucially,TNDDS can actively drive the charged droplets of prepared triangular silver nanoprisms(Ag NPs)forward and back to mix with and remove a tiny bacterial droplet on an open SDP or in a tiny semi-enclosed channel.Bacteria could be killed by releasing Ag+and effectively removed by TNDDS by regulating the motion direction.Generally,this approach offers a promising application for removing bacteria from material surfaces driven by TENG and opens a new avenue for bacterial anti-adhesion. | Jing Lin Jialin Li Shichang Feng Caiqin Gu Huajian Li Hanqing Lu Fei Hu Duo Pan Ben Bin Xu Zhanhu Guo | 2023 | Nano Research2023，16，1： | 0 |
| 17 | Investigation on Atomization Performance of a Slinger显示文摘Slinger is a type of rotary atomizer wherein spray spatial distribution varies with the rotary speed and mass flowrate.The spray parameters at a series of locations in the radial direction of the slinger were measured and the parameters of the location where the spray Sauter Mean Diameter(SMD)corresponds to the smallest were selected to be analyzed.The results indicated that at a specific mass flowrate,an optimum diameter exists for the orifice,in which the orifice’s spray corresponds to the smallest and most uniform.Increases in the mass flowrate increase the optimum diameter.Further,the spray from the circular orifice is more uniform compared with that from the slot orifice with the same circumference.When the length of the orifice increases,the spray becomes larger but more uniform.The liquid regime in the orifice affects primary atomization of the liquid,significantly affecting the spray SMD and uniformity. | Wenlong Tan Weijun Fan Qiang Shi Hanqing Xu Rongchun Zhang | 2021 | Journal of Harbin Institute of Technology(New Series)2021，28，6： | 0 |
| 18 | Stability-constrained Two-stage Robust Optimization for Integrated Hydrogen Hybrid Energy System显示文摘In order to cope with the challenges brought by multiple uncertainties to integrated hydrogen hybrid energy systems,a stability-constrained two-stage robust optimization method considering small disturbance stability and characteristics of dynamic response is proposed in this paper.In the first operating stage,the charging/discharging state of the battery and the startstop state of the electrolyzer and fuel cell are determined.Then,power constraints for stabilizing ESSs power output is considered between the first and second stage optimization to improve the small disturbance stability of the robust operation plan.Then,the goal of minimizing the operation cost and determine a robust operation operating plan under the worst case is conducting in the second stage optimization.Through the small-signal model with time-delay effect,the eigenvalue analysis method is used to find the ESSs power range,and the small-signal stability of obtained robust operation plan can be enhanced.Finally,the effectiveness and superiority of the proposed method are proved by comparing with the traditional static robust optimization method.Impacts of uncertain parameters on economy and stability are also investigated in a typical example. | Qi Li Yibin Qiu Hanqing Yang Yan Xu Weirong Chen Peng Wang | 2021 | CSEE Journal of Power and Energy Systems2021，7，1： | 0 |
| 19 | Perspectives on Compound Flooding in Chinese Estuary Regions显示文摘Extreme surges and rainfall represent major driving factors for compound flooding in estuary regions along the Chinese coast.The combined effect of extreme surges and rainfall(that is,compound floods)might lead to greater impacts than if the drivers occurred in isolation.Hence,understanding the frequency and severity of compound flooding is important for improving flood hazard assessment and compound flood resilience in coastal cities.In this study,we examined the dependence between extreme surges and corresponding rainfall events in 26 catchments along the Chinese coastline during typhoon and non-typhoon seasons using copula functions,to identify where the two drivers more often occur together and the implication for flood management in these locations.We found that the interaction between flood drivers is statistically significant in 10catchments located around Hainan Island(south)and Shanghai,where surge peaks occur mainly during the typhoon season and around the Bohai Sea(north),where surge peaks occur mainly during the non-typhoon season.We further applied the copula-based framework to model the dependence between surge peaks and associated rainfall and estimate their joint and conditional probability in two specific locations—Hainan Island and the Bohai Sea,where the correlation between flood drivers is statistically significant.We observed that in Hainan Island where most of the surge peaks occur during the typhoon season,extreme rainfall events during the typhoon season are generally more intense compared to annual maxima rainfall.In contrast,around the Bohai Sea where surge peaks occur mainly outside the typhoon season,rainfall is less intense than annual maxima rainfall.These results show that the interaction between extreme surges and rainfall can provide valuable insight when designing coastal and urban infrastructure,especially in highly populated urban areas prone to both coastal and pluvial flooding,such as many Chinese coastal cities. | Hanqing Xu Elisa Ragno Jinkai Tan Alessandro Antonini Jeremy D.Bricker Sebastiaan N.Jonkman Qing Liu Jun Wang | 2023 | International Journal of Disaster Risk Science2023，14，2： | 0 |
| 20 | MORPHOLOGICAL STUDY ON VERTEBARTE MELANOCYTES显示文摘In order to explore the development of melanocytes (MC) in comparative systematic biology, so as to make a further understanding of the function of MC and diseases related to MC, we made a comparative study through pathological and TEM technique, on morphology of MC in different vertebrate animals, such as fish class (finel eel), amphibious animal (matured and immatured bulldog and frog), reptilian (turtle, walzard), aves (chicken) and mammalia (white mice, human embryo and adult in formative process of vertibate evolution.). The results showed that histologically, from the fish to amphibious animal, reptilia, and mammalia. MC were increased in number gradually, forming from stellar, polygonal form to more regular form with cell body growing smaller and dendrites growing from pyknic to slender with a gradual increase of branches, MC also scattreed differently and the organells developed more with evolution. These suggest that the change of MC in the evolution process of vertebrates may be related to the gradual adapte to alteration of living environment from water to land. As to the sunlight and ultraviolet ray increases. It also proves, on the other hand, that MC has the effect of blocking or to get shed of the sun. | Xu Hanqing,Song Changsuo,Su Baoshan, Ren Junfeng Department of Dermatology, Second Affiliated Hospital, Xi′an Medical University, Xi′an 710004 | 1999 | Journal of Pharmaceutical Analysis1999，13，2： | 0 |
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