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| 1 | A community-derived classification for extant lycophytes and ferns显示文摘发展史长通知了蕨类植物分类。当我们推断进化的树的能力改善了,针对认出生来的组的分类变得逐渐地预兆、稳定。这里,我们为 lycophytes 和蕨纲植物提供一个现代、全面分类,在下面,利用一条基于社区的途径类水平。我们 monophyly 用作主要标准让 taxa,而且目的识别保存两个广泛地被接受的存在 taxa 和界限并且与我们蕨类植物发展史的理解一致。总共,这个分类对待一在 337 个类, 51 个家庭, 14 目,和二个班估计了 11 916 种类。这个分类没在 lycophyte 和蕨纲植物上作为最后的词被打算分类,而是当前的假设的概括陈述,源于最好的可得到的数据并且在问题由熟悉植物的那些大多数塑造了。我们希望它将在蕨类植物上为最近的文学的那些想要的参考用作一个资源发展史和分类,为指导未来调查的一个框架,和推进讲话的刺激。 | Eric Schuettpelz Harald Schneider Alan R. Smith Peter Hovenkamp Jefferson Prado Germinal Rouhan Alexandre Salino Michael Sundue Thafs Elias Almeida Barbara Parris Emily B. Sessa Ashley R. Field Andre Luis de Gasper Carl J. Rothfels Michael D. Windham Marcus Lehnert Benjamin Dauphin Atsushi Ebihara Samuli Lehtonen Pedro Bond Schwartsburd Jordan Metzgar Li-Bing Zhang Li-Yaung Kuo Patrick J. Brownsey Masahiro Kato Marcelo Daniel Arana Francine C. Assis Michael S. Barker David S. Barrington Ho-Ming Chang Yi-Han Chang Yi-Shan Chao Cheng-Wei Chen De-Kui Chen Wen-Liang Chiou Vinicius Antonio de Oliveira Dittrich Yi-Fan Duan Jean-Yves Dubuisson Donald R. Farrar Susan Fawcett Jose Maria Gabriel y Galan Luiz Armando de Araujo Goes-Neto Jason R. Grant Amanda L. Grusz Christopher Haufler Warren Hauk Hai He Sabine Hennequin Regina Yoshie Hirai Layne Huiet Michael Kessler Petra Korall Paulo H. Labiak Anders Larsson Blanca Leen Chun-Xiang Li Fay-Wei Li Melanie Link-Perez Hong-Mei Liu Ngan Thi Lu Esteban I. Meza-Torres Xin-Yuan Miao Robbin Moran Claudine Massi Mynssens Nathalie Nagalingum Benjamin Ollgaard Alison M. Paul Jovani B. de S. Pereira Leon R. Perrie Monica Ponce Tom A. Ranker Christian Schulz Wataru Shinohara Alexander Shmakov Erin M. Sigel Filipe Soares de Souza Lana da Silva Sylvestre Weston Testo Luz Amparo Triana-Moreno Chie Tsutsumi Hanna Tuomisto IvAn A. Valdespino Alejandra Vasco Raquel Stauffer Viveros Alan Weakley Ran Wei Stina Weststrand Paul G. Wolf George Yatskievych Xiao-Gang Xu Yue-Hong Yan Liang Zhang Xian-Chun Zhang Xin-Mao Zhou | 2016 | Journal of Systematics and Evolution2016，54，6： | 44 |
| 2 | A life support-based comprehensive treatment regimen dramatically lowers the in-hospital mortality of patients with fulminant myocarditis: a multiple center study显示文摘Fulminant myocarditis(FM) has unacceptable high mortality. This study aimed to evaluate the therapeutic efficacy of a life support-based comprehensive treatment regimen(LSBCTR), a completely novel treatment regimen, for FM. A total of 169 FM patients recruited from January 2008 to December 2018 were divided into two groups: patients receiving LSBCTR(81 cases),which includes(i) mechanical life support(positive pressure respiration, intra-aortic balloon pump with or without extracorporeal membrane oxygenation),(ii) immunomodulation therapy using sufficient doses of glucocorticoids and immunoglobulins, and(iii) application of neuraminidase inhibitors, and those receiving conventional treatment(88 cases). The endpoints were in-hospital death and heart-transplantation. Of all the population, 44 patients(26.0%) died in hospitals. Inhospital mortality was 3.7%(3/81) for LSBCTR group and 46.6%(41/88) for traditional treatment(P<0.001). Early application of LSBCTR, mechanical life support, neuraminidase inhibitors, and immunomodulation therapy significantly contributed to reduction in in-hospital mortality. This study describes a novel treatment regimen for FM patients that dramatically reduces inhospital mortality. Its generalization and clinical application will efficiently save lives although further optimization is needed.This study offers an insight that virus infection induced inflammatory waterfall results in cardiac injury and cardiogenic shock and is the therapeutic target. | Sheng Li Shengyong Xu Chenze Li Xiao Ran Guanglin Cui Mengying He Kun Miao Chunxia Zhao Jiangtao Yan Rutai Hui Ning Zhou Yan Wang Jiangang Jiang Jing Zhang Daowen Wang | 2019 | Science China(Life Sciences)2019，62，3： | 43 |
| 3 | Effect of oxymatrine on the replication cycle of hepatitis B virus in vitro显示文摘AIM:To determine the antiviral mechanism or target of oxymatrine against hepatitis B virus(HBV).METHODS:HepG2.2.15 cells were incubated with culture medium containing 500 μg/mL of oxymatrine for 2 and 5 d.The surface antigen of HBV(HBsAg) and e antigen of HBV(HBeAg) in supernatant were determined by ELISA.HBV DNA in supernatant,and intracellular covalently closed circular DNA(cccDNA),relaxed circular DNA(rcDNA) and pregenomic RNA(pgRNA) were quantif ied by specif ic real-time polymerase chain reaction(PCR) or reverse transcription(RT)-PCR.RESULTS:Treatment with oxymatrine for 2 d and 5 d reduced the production of HBV by the cell line,as indicated by the decline of HBsAg(22.67%,t = 5.439,P = 0.0322 and 22.39%,t = 5.376,P = 0.0329,respectively),HBeAg(55.34%,t = 9.859,P = 0.0101 and 43.97%,t = 14.080,P = 0.0050) and HBV DNA(40.75%,t = 4.570,P = 0.0447 and 75.32%,t = 14.460,P = 0.0047) in the supernatant.Intracellular cccDNA was also markedly reduced by 63.98%(t = 6.152,P = 0.0254) and 80.83%(t = 10.270,P = 0.0093),and intracellular rcDNA by 34.35%(t = 4.776,P = 0.0413) and 39.24%(t = 10.050,P = 0.0097).In contrast,intracellular pgRNA increased by 6.90-fold(t = 8.941,P = 0.0123) and 3.18-fold(t = 7.432,P = 0.0176) after 500 μg/mL of oxymatrine treatment for 2 d and 5 d,respectively.CONCLUSION:Oxymatrine may inhibit the replication of HBV by interfering with the process of packaging pgRNA into the nucleocapsid,or inhibiting the activity of the viral DNA polymerase. | Xu, Wen-Sheng Zhao, Ke-Kai Miao, Xiao-Hui Ni, Wu Cai, Xiong Zhang, Rui-Qi Wang, Jun-Xue | 2010 | World Journal of Gastroenterology2010，16，16： | 27 |
| 4 | Cryo-electron microscopy structures of the SARS-CoV spike glycoprotein reveal a prerequisite conformational state for receptor binding显示文摘在 2002-2003 的 SARS 的全球爆发被新人的 coronavirus SARS-CoV 的感染引起。SARS-CoV 的感染主要通过病毒的表面 glycoproteins 被调停,它由 S1 和 S2 子单元组成并且在 virions 的信封上形成更整齐的尖铁。这里,我们报导处于单个粒子的 cryo 电子显微镜学决定的不同 conformational 状态更整齐的 SARS-CoV 表面尖铁的 ectodomain 结构。1 在 4.3 点决定了的符合构造?分辨率是三方面的对称并且有所有三受体绑定 C 终端域 1 (CTD1s ) S1 子单元在在位置下面。绑定下面到 SARS-CoV 受体 ACE2 的 CTD1s 不由于位的碰撞是可能的,建议 1 代表的符合构造受体绑定不活跃的状态。在 7.3, 5.7 和 6.8 点坚定的符合构造 2-4?决定都是不对称的,在哪个 RBD 旋转离开下面由不同角度到的位置一起来位置。起来 CTD1 为 ACE2 约会暴露受体绑定地点,建议符合构造 2-4 代表受体绑定活跃状态。这个 conformational 变化也为抵销指向 CTD1 的抗体的 SARS-CoV 的绑定被要求。这现象能被扩大到为受体绑定利用 S1 子单元的 CTD1 的另外的 betacoronaviruses,它提供新卓见进在感染期间更整齐的 coronavirus 熔化前尖铁的中间的状态。 | Miao Gui Wenfei Song Haixia Zhou Jingwei Xu Silian Chen Ye Xiang XinquanWang | 2017 | Cell Research2017，27，1： | 25 |
| 5 | Nomogram to predict overall survival after gallbladder cancer resection in China显示文摘AIM To integrate clinically significant variables related to prognosis after curative resection for gallbladder carcinoma(GBC) into a predictive nomogram.METHODS One hundred and forty-two GBC patients who underwent curative intent surgical resection at Peking Union Medical College Hospital(PUMCH) were included. This retrospective case study was conducted at PUMCH of the Chinese Academy of Medical Sciences and Peking Union Medical College(CAMS & PUMC) in China from January 1, 2003 to January 1, 2018. The continuous variable carbohydrate antigen 19-9(CA19-9) was converted into a categorical variable(cCA19-9) based on the normal reference range. Stages 0 to IIIA were merged into one category, while the remaining stages were grouped into another category. Pathological grade X(GX) was treated as a missing value. A multivariate Cox proportional hazards model was used to select variables to construct a nomogram. Discrimination and calibration of the nomogram were performed via the concordance index(C-index) and calibration plots. The performance of the nomogram was estimated using the calibration curve. Receiver operating characteristic(ROC) curve analysis and decision curve analysis(DCA) were performed to evaluate the predictive accuracy and net benefit of the nomogram, respectively.RESULTS Of these 142 GBC patients, 55(38.7%) were male, and the median and mean age were 64 and 63.9 years, respectively. Forty-eight(33.8%) patients in this cohort were censored in the survival analysis. The median survival time was 20 months. A series of methods, including the likelihood ratio test and Akaike information criterion(AIC) as well as stepwise, forward, and backward analyses, were used to select the model, and all yielded identical results. Jaundice [hazard ratio(HR) = 2.9; 95% confidence interval(CI): 1.60-5.27], cCA19-9(HR = 3.2; 95%CI: 1.91-5.39), stage(HR = 1.89; 95%CI: 1.16-3.09), and resection(R)(HR = 2.82; 95%CI: 1.54-5.16) were selected as significant predictors and combined into a survival time predictive nomogram(C-index = 0.803; 95%CI: 0.766-0.839). High prediction accuracy(adjusted C-index = 0.797) was further verified via bootstrap validation. The calibration plot demonstrated good performance of the nomogram. ROC curve analysis revealed a high sensitivity and specificity. A high net benefit was proven by DCA.CONCLUSION A nomogram has been constructed to predict the overall survival of GBC patients who underwent radical surgery from a clinical database of GBC at PUMCH. | Yi Bai Zhi-Song Liu Jian-Ping Xiong Wei-Yu Xu Jian-Zhen Lin Jun-Yu Long Fei Miao Han-Chun Huang Xue-Shuai Wan Hai-Tao Zhao | 2018 | World Journal of Gastroenterology2018，24，45： | 25 |
| 6 | A putative 6-transmembrane nitrate transporter OsNRT1.1b plays a key role in rice under low nitrogen显示文摘OsNRT1.1a is a low-affinity nitrate(NO_3^-) transporter gene. In this study, another mRNA splicing product, OsNRT1.1b,putatively encoding a protein with six transmembrane domains, was identified based on the rice genomic database and bioinformatics analysis. OsNRT1.1a/OsNRT1.1b expression in Xenopus oocytes showed OsNRT1.1a-expressing oocytes accumulated ^(15)N levels to about half as compared to OsNRT1.1bexpressing oocytes. The electrophysiological recording of OsNRT1.1b-expressing oocytes treated with 0.25 mM NO_3^- confirmed ^(15)N accumulation data. More functional assays were performed to examine the function of OsNRT1.1b in rice. The expression of both OsNRT1.1a and OsNRT1.1b was abundant in roots and downregulated by nitrogen(N) deficiency. The shoot biomass of transgenic rice plants with OsNRT1.1a or OsNRT1.1b overexpression increased under various N supplies under hydroponic conditions compared to wild-type(WT). The OsNRT1.1a overexpression lines showed increased plant N accumulation compared to the WT in 1.25 mM NH_4NO_3 and 2.5 mM NO_3^- or NH_4^+ treatments, but not in 0.125 mM NH_4NO_3.However, OsNRT1.1b overexpression lines increased total N accumulation in all N treatments, including 0.125 m M NH_4NO_3,suggesting that under low N condition, OsNRT1.1b would accumulate more N in plants and improve rice growth, but also that OsNRT1.1a had no such function in rice plants. | Xiaorong Fan Huimin Feng Yawen Tan Yanling Xu Qisong Miao Guohua Xu | 2016 | Journal of Integrative Plant Biology2016，58，6： | 21 |
| 7 | Inhibition of 12-lipoxygenase reduces proliferation and induces apoptosis of hepatocellular carcinoma cells in vitro and in vivo显示文摘BACKGROUND:12-lipoxygenase(12-LOX) has been reported to be an important gene in cancer cell proliferation and survival,and tumor metastasis.However,its role in hepatocellular carcinoma(HCC) cells remains unknown.METHODS:Expression of 12-LOX was assessed in a diethylnitrosamine-induced rat HCC model,and in SMMC-7721,HepG2 and L-02 cells using immunohistochemical staining and reverse transcriptase-polymerase chain reaction(RT-PCR).GST-π and Ki-67 were determined in vivo by immunohistochemical staining.Apoptosis was evaluated by TUNEL assay.Cell viability and apoptosis were determined by MTT assay and flow cytometry,respectively.Apoptosis-related proteins in SMMC-7721 and HepG2 cells were detected by Western blotting.RESULTS:Immunohistochemical staining and RT-PCR showed that 12-LOX was over-expressed in rat HCC and two HCC cell lines,while the expression was inhibited by baicalein,a specific inhibitor of 12-LOX.Baicalein inhibited cell proliferation and induced apoptosis in rat HCC and both cell lines in a dose-and time-dependent manner.Our in vivo study demonstrated that baicalein also reduced neoplastic nodules.Mechanistically,baicalein reduced Bcl-2 protein expression coupled with a slight increase of the expression of Bax and activation of caspase-3.Furthermore,baicalein inhibited the activation of ERK-1/2(phosphorylated).Interestingly,the effects of baicalein were reversed by 12(S)-HETE,a metabolite of 12-LOX.CONCLUSIONS:Inhibition of 12-LOX leads to reduced numbers of HCC cells,partially caused by increased apoptosis.12-LOX may be a potential molecular target for HCC prevention and treatment. | Xi-Ming Xu,Guang-Jin Yuan,Jun-Jian Deng,Hong-Ting Guo,Miao Xiang,Fang Yang,Wei Ge and Shi-You Chen Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China Cancer Center, the 82nd Hospital of the Chinese PLA, Huai’an 223001, China Department of Physiology, Medical College of Wuhan University, Wuhan 430071, China Department of Physiology & Pharmacology, University of Georgia, Athens, GA 30602, USA | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，2： | 20 |
| 8 | Antigen-presenting effects of effector memory Vγ9Vδ2 T cells in rheumatoid arthritis显示文摘 | Chaoying Hu Liu Qian Yi Miao Qiuyu Huang Ping Miao Ping Wang Qiwen Yu Hong Nie Jiying Zhang Dongyi He Rong Xu Xuehua Chen Bingya Liu Dongqing Zhang | 2012 | Cellular & Molecular Immunology2012，9，3： | 18 |
| 9 | Paclitaxel ameliorates fibrosis in hepatic stellate cells via inhibition of TGF-β/Smad activity显示文摘AIM: To investigated if paclitaxel can attenuate hepatic fi brosis in rat hepatic stellate cells (RHSCs). METHODS: RHSCs were cultured in vitro and randomly assigned to four groups: normal control group (treated only with Dulbecco's Modified Eagle's Medium), Taxol group (200 nmol/L paclitaxel was added to the cell culture), transforming growth factor (TGF)-β group (5 ng/mL recombinant human TGF-β1 was added to the cell culture), and TGF-β + Taxol group. TGF-β signaling cascade and status of various extracellular matrix proteins were evaluated by real time reverse transcriptase polymerase chain reaction and Western blotting. RESULTS: The paclitaxel treatment markedly suppressed Smad2/3 phosphorylation. This was associated with attenuated expression of collagen Ⅰ and Ⅲ and fi bronectin in RHSCs.CONCLUSION: These data indicate that 200 nmol/L paclitaxel ameliorates hepatic fi brosis via modulating TGF-β signaling, and that paclitaxel may have some therapeutic value in humans with hepatic fi brosis. | Jun Zhou, De-Wu Zhong, Qun-Wei Wang, Xiong-Ying Miao, Xun-Di Xu, Department of Hepatobiliary Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China | 2010 | World Journal of Gastroenterology2010，16，26： | 17 |
| 10 | Astragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosis显示文摘AIM: To determine the inhibitory effect of Astragalus memebranaceushas on gastric cancer cell supernatantinduced apoptosis of human peritoneal mesothelial cells. METHODS: Human peritoneal mesothelial cell (HPMC) line HMrSV5 was co-incubated with gastric cancer cell supernatant (MKN45) and/or Astragalus memebranaceushas. Morphological changes in gastric cancer cells were observed under phase-contrast microscope. Quantitative cell damage was determined by MTT assay. Apoptosis was determined under transmission electron microscope and quantified by detecting acridine orange/ethidium bromide-stained (AO/EB) condensed nuclei under fluorescent microscope or by flow cytometry. Expressions of Bcl-2 and Bax were evaluated with immunostaining. RESULTS: Morphological changes and exfoliation occurred and naked areas appeared in cultured HMrSV5 cells 24 h after they were treated with gastric cancer cell supernatant. Cell supernatant from MKN45 gastric cancer cells induced apoptosis of HMrSV5 cells in a time-dependent manner. Obvious morphological changes were observed in cell apoptosis, such as condensation of chromatin, nuclear fragmentations and apoptotic bodies. Astragalus memebranaceus could partly suppress these changes and regulate the expressions of Bcl-2 and Bax in HMrSV5 cells.CONCLUSION: Gastric cancer cells induce apoptosis of HPMCs through the supernatant. Astragalus memebranaceushas inhibits this phenomenon and can be used an adjuvant chemothera-peutic agent in gastric cancer therapy. | Di Na Fu-Nan Liu Zhi-Feng Miao Zong-Min Du Hui-Mian Xu | 2009 | World Journal of Gastroenterology2009，15，5： | 17 |
| 11 | Hypoglycemic effect of catalpol on high-fat diet/streptozotocin-induced diabetic mice by increasing skeletal muscle mitochondrial biogenesis显示文摘Catalpol, iridoid glycoside,在根值 Rehmanniae 的根存在。一些研究证明了 catalpol 为这效果在导致 streptozotocin 的糖尿病的模型,而是内在的机制有显著血糖过低的效果充分没被阐明。因为 mitochondrial 机能障碍在糖尿病的病理起一个重要作用并且因为改善 mitochondrial 功能可以为糖尿病的处理提供一条新途径,这研究被设计。Catalpol 口头上地予导致的高脂肪的 diet/streptozotocin (HFD/STZ ) 和 metformin 被以为 4 个星期的糖尿病的老鼠日报。身体重量(BW )(IPGTT ) , fasting 血葡萄糖(FBG ) 水平,和葡萄糖处理被测量在期间或在处理以后。结果整个 catalpol 管理的四个连续星期在 BW 没有明显的变化显示出 FBG 水平的剂量依赖者减小。Catalpol 治疗实质地在糖尿病的老鼠减少了浆液总数胆固醇和 triglyceride 层次。另外, catalpol 高效地增加了 mitochondrial ATP 生产并且在骨胳的肌肉织物颠倒了 mitochondrial 膜潜力和 mtDNA 拷贝数字的减少。而且, catalpol (200 mg/kg ) 在骨胳的肌肉救了 mitochondrial 超微结构,与传播电子显微镜学检测了。peroxisome 的相对 mRNA 水平(PGC1 ) 当这效果被 catalpol 颠倒时,激活 proliferator 的受体 gamma 激活剂 1 显著地在糖尿病的老鼠的肌肉组织被减少,导致一条剂量依赖者起来规定。一起拿,我们发现 catalpol 能够经由在 HFD/STZ-induced 糖尿病的老鼠的骨胳的肌肉改进 mitochondrial 功能的阴沉的 FBG 水平。 | Xia Li Zhimeng Xu Zhenzhou Jiang Lixin Sun Jinzi Ji Jingshan Miao Xueji Zhang Xiaojie Li Shan Huang Tao Wang Luyong Zhang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，9： | 16 |
| 12 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 13 | Research progress on synergistic anti-tumor mechanisms of compounds inTraditional Chinese Medicine显示文摘The curative effects of Chinese herbal compounds result from the coordination of numerous natural compounds.We aimed to review the mechanism of action of Traditional Chinese Medicine(TCM)compounds(TCMC),explore the rationality of formulation theory and synergistic effects in TCM compounds,and analyze the effectiveness of drug compatibility of TCMC in molecular biology.This literature review covers the mechanisms of the anti-tumor effects of compounds,and their synergistic antitumor mechanisms.We aim to provide reference for the effective development and use of natural resources and the organic combination of TCM and modern medicine using molecular biology. | Jing Zhou Tianyao Zhou Meijuan Chen Miao Jiang Xiaoxia Wang Qin Liu Zhen Zhan Xu Zhang | 2014 | Journal of Traditional Chinese Medicine2014，34，1： | 15 |
| 14 | Combining CLUE-S and SWAT Models to Forecast Land Use Change and Non-point Source Pollution Impact at a Watershed Scale in Liaoning Province, China显示文摘Non-point source(NPS) pollution has become a major source of water pollution. A combination of models would provide the necessary direction and approaches designed to control NPS pollution through land use planning. In this study, NPS pollution load was simulated in urban planning, historic trends and ecological protection land use scenarios based on the Conversion of Land Use and its Effect at Small regional extent(CLUE-S) and Soil and Water Assessment Tool(SWAT) models applied to Hunhe-Taizi River Watershed, Liaoning Province, China. Total nitrogen(TN) and total phosphorus(TP) were chosen as NPS pollution indices. The results of models validation showed that CLUE-S and SWAT models were suitable in the study area. NPS pollution mainly came from dry farmland, paddy, rural and urban areas. The spatial distribution of TN and TP exhibited the same trend in 57 sub-catchments. The TN and TP had the highest NPS pollution load in the western and central plains, which concentrated the urban area and farm land. The NPS pollution load would increase in the urban planning and historic trends scenarios, and would be even higher in the urban planning scenario. However, the NPS pollution load decreased in the ecological protection scenario. The differences observed in the three scenarios indicated that land use had a degree of impact on NPS pollution, which showed that scientific and ecologically sound construction could effectively reduce the NPS pollution load in a watershed. This study provides a scientific method for conducting NPS pollution research at the watershed scale, a scientific basis for non-point source pollution control, and a reference for related policy making. | LIU Miao LI Chunlin HU Yuanman SUN Fengyun XU Yanyan CHEN Tan | 2014 | Chinese Geographical Science2014，24，5： | 15 |
| 15 | Aggravated ulcerative colitis caused by intestinal Metrnl deficiency is associated with reduced autophagy in epithelial cells显示文摘Metrnl is a newly identified secreted protein highly expressed in the intestinal epithelium.This study aimed to explore the role and mechanism of intestinal epithelial Metrnl in ulcerative colitis.Metrnl-/-(intestinal epithelial cell-specific Metrnl knockout)mice did not display any phenotypes of colitis under basal conditions.However,under administration of 3%dextran sodium sulfate(DSS)drinking water,colitis was more severe in Metrnl-/-mice than in WT mice,as indicated by comparisons of body weight loss,the presence of occult or gross blood per rectum,stool consistency,shrinkage in the colon,intestinal damage,and serum levels of inflammatory factors.DSS-induced colitis activated autophagy in the colon.This activation was partially inhibited by intestinal epithelial Metrnl deficiency,as indicated by a decrease in Beclin-1 and LC3-Ⅱ/Ⅰand an increase in p62 in DSS-treated Metrnl-/-mice compared with WT mice.These phenomena were further confirmed by observation of autophagosomes and immunofluorescence staining for LC3 in epithelial cells.The autophagy-related AMPK-mTOR-p70S6K pathway was also activated in DSS-induced colitis,and this pathway was partially blocked by intestinal epithelial Metrnl deficiency,as indicated by a decrease in AMPK phosphorylation and an increase in mTOR and p70S6K phosphorylation in DSS-treated Metrnl-/-mice compared with WT mice.Therefore,Metrnl deficiency deteriorated ulcerative colitis at least partially through inhibition of autophagy via the AMPK-mTOR-p70S6K pathway,suggesting that Metrnl is a therapeutic target for ulcerative colitis. | Sai-long Zhang Zhi-yong Li Dong-sheng Wang Tian-ying Xu Mao-bing Fan Ming-he Cheng Chao-yu Miao | 2020 | Acta Pharmacologica Sinica2020，41，6： | 14 |
| 16 | Staged carotid artery angioplasty and stenting for patients with high-grade carotid stenosis with high risk of developing hyperperfusion injury:a retrospective analysis of 44 cases显示文摘Background:Hyperperfusion syndrome(HPS)is a rare but potentially a life-threatening complication after carotid artery angioplasty and stenting(CAS).Staged CAS has been an alternative to prevent HPS.Materials and methods:44 of 908 patients with high-grade internal carotid artery stenosis or near occlusion were at risk of HPS because of poor collateral flow and impaired cerebral blood flow(CBF).They were treated with first(stage 1),followed by a full CAS(stage 2)1 month later.Their 30-day outcomes were tabulated and analysed.Results:During follow-up,1 of the 44(2.2%)patients developed HPS immediately,3(7%)had postprocedural HPS(ie,transcranial Doppler(TCD)>120%)without clinical symptoms and 3(7%)required stenting at stage 1 for carotid dissections.After stage 1,there were significant improvement between the preprocedural and postprocedural CBF(0.98±0.06 vs 0.85±0.05,p<0.05),mean transit time(MTT;1.05±0.05 vs 1.15±0.05,p<0.05),time to peak(TTP;1.04±0.06 vs 1.20±0.06,p<0.05)on CT perfusion(CTP),and CBF(66.41±7.41 vs 44.44±6.43,p<0.05)on TCD.After stage 2,improvement was seen in CBF(1.01±0.07 vs 0.98±0.06,p<0.05),MTT(1.01±0.05 vs 1.05±0.05,p<0.05),TTP(0.99±0.06 vs 1.04±0.06,p<0.05)on CTP and CBF(66.41±7.41 vs 93.78±18.81,p<0.05)on TCD.2 had postoperative increase of middle cerebral artery mean flow velocity of 120%after stage 2 without clinical symptoms.Conclusion:Staged carotid artery stenting probably decreased the chance of developing HPS in this group of selected patients.Although requiring a 2-step intervention,staged CAS may be a safe and effective alternative. | Dapeng Mo Gang Luo Bo Wang Ning Ma Feng Gao Xuan Sun Xiaotong Xu Zhongrong Miao | 2016 | Stroke & Vascular Neurology2016，1，4： | 14 |
| 17 | Neuroprotective effects of autophagy inhibition on hippocampal glutamate receptor subunits after hypoxia-ischemia-induced brain damage in newborn rats显示文摘Autophagy has been suggested to participate in the pathology of hypoxic-ischemic brain damage(HIBD).However,its regulatory role in HIBD remains unclear and was thus examined here using a rat model.To induce HIBD,the left common carotid artery was ligated in neonatal rats,and the rats were subjected to hypoxia for 2 hours.Some of these rats were intraperitoneally pretreated with the autophagy inhibitor 3-methyladenine(10 m M in 10 μL) or the autophagy stimulator rapamycin(1 g/kg) 1 hour before artery ligation.Our findings demonstrated that hypoxia-ischemia-induced hippocampal injury in neonatal rats was accompanied by increased expression levels of the autophagy-related proteins light chain 3 and Beclin-1 as well as of the AMPA receptor subunit GluR 1,but by reduced expression of GluR 2.Pretreatment with the autophagy inhibitor 3-methyladenine blocked hypoxia-ischemia-induced hippocampal injury,whereas pretreatment with the autophagy stimulator rapamycin significantly augmented hippocampal injury.Additionally,3-methyladenine pretreatment blocked the hypoxia-ischemia-induced upregulation of Glu R1 and downregulation of GluR2 in the hippocampus.By contrast,rapamycin further elevated hippocampal Glu R1 levels and exacerbated decreased GluR2 expression levels in neonates with HIBD.Our results indicate that autophagy inhibition favors the prevention of HIBD in neonatal rats,at least in part,through normalizing Glu R1 and GluR2 expression. | Li-xiao Xu Xiao-juan Tang Yuan-yuan Yang Mei Li Mei-fang Jin Po Miao Xin Ding Ying Wang Yan-hong Li Bin Sun Xing Feng | 2017 | Neural Regeneration Research2017，12，3： | 12 |
| 18 | Nicotinamide phosphoribosyltransferase aggravates inflammation and promotes atherosclerosis in ApoE knockout mice显示文摘Nicotinamide phosphoribosyltransferase (Nampt) is the rate-limiting enzyme of nicotinamide adenine dinucleotide (NAD) salvage biosynthesis in mammals, and is involved in fundamental physiological processes and pathophysiology of many diseases. Thus far, however, the role of Nampt in atherosclerosis development is still in debate. In this study, we crossed global Nampt transgenic mice (Nampt-Tg) with a well-established atherosclerosis animal model (ApoE knockout mice, ApoE-/-) to generate ApoE-/-;Nampt-Tg mice and investigated the effects of Nampt overexpression on atherosclerosis development in ApoE-/- mice. Both ApoE-/- and ApoE-/-;Nampt-Tg mice were fed with a pro-atherosclerotic high-fat diet (HFD) for 16 weeks. Their serum lipid contents and atherosclerotic lesion were assessed. The results showed that there was no significant difference in body weight or serum levels of glucose, total cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol between the two strains of mice, but ApoE-/-;Nampt-Tg mice had a significantly higher level of serum non-esterified fatty acid. Compared with ApoE-/- mice, ApoE-/-;Nampt-Tg mice displayed significantly increased atherosclerotic lesion area and thickness, lower collagen content, decreased collagen I/III ratio (collagen immaturation), increased number of apoptotic cells, and enhanced activities of caspase-3, caspase-8, and caspase-9. Moreover, macrophage infiltration (F4/80 staining), tumor necrosis factor signaling, and chemokines expression (ICAM-1 and CXCR-4) were all activated in aortic atherosclerotic plaque of ApoE-/-;Nampt-Tg mice compared with ApoE-/- mice. Our results provide in vivo evidence that Nampt transgene aggravates atherosclerotic inflammation and promotes atherosclerosis development in ApoE-/- mice. | Yuan-yuan Kong Guo-qiang Li Wen-jie Zhang Xia Hua Can-can Zhou Tian-ying Xu Zhi-yong Li Pei Wang Chao-yu Miao | 2019 | Acta Pharmacologica Sinica2019，40，9： | 12 |
| 19 | Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells显示文摘Objective:We investigated the effects of intermittent negative pressure on osteogenesis in human bone marrowderived stroma cells(BMSCs) in vitro. Methods:BMSCs were isolated from adult marrow donated by a hip osteoarthritis patient with prosthetic replacement and cultured in vitro. The third passage cells were divided into negative pressure treatment group and control group. The treatment group was induced by negative pressure intermittently(pressure:50 kPa,30 min/times,and twice daily). The control was cultured in conventional condition. The osteogenesis of BMSCs was examined by phase-contrast microscopy,the determination of alkaline phosphatase(ALP) activities,and the immunohistochemistry of collagen type I. The mRNA expressions of osteoprotegerin(OPG) and osteoprotegerin ligand(OPGL) in BMSCs were analyzed by real-time polymerase chain reaction(PCR). Results:BMSCs showed a typical appearance of osteoblast after 2 weeks of induction by intermittent negative pressure,the activity of ALP increased significantly,and the expression of collagen type I was positive. In the treatment group,the mRNA expression of OPG increased significantly(P<0.05) and the mRNA expression of OPGL decreased significantly(P<0.05) after 2 weeks,compared with the control. Conclusion:Intermittent negative pressure could promote osteogenesis in human BMSCs in vitro. | Zhi YANG Miao LIU Yin-gang ZHANG Xiong GUO Peng XU | 2009 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2009，10，3： | 12 |
| 20 | Distribution of Major Pathogens from Sputum and Bronchoalveolar Lavage Fluid in Patients with Noncystic Fibrosis Bronchiectasis: A Systematic Review显示文摘 | Xia-Yi Miao Xiao-Bin Ji Hai-Wen Lu Jia-Wei Yang Jin-Fu Xu | 2015 | Chinese Medical Journal2015，，20： | 12 |
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