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| 1 | Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution显示文摘The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits,which is of fundamental importance for neuroscience research.Here,we describe a new microscopy method of Volumetric Imaging with Synchronized on-the-fly-scan and Readout(VISo R)for high-throughput,high-quality brain mapping.Combining synchronized scanning beam illumination and oblique imaging over cleared tissue sections in smooth motion,the VISo R system effectively eliminates motion blur to obtain undistorted images.By continuously imaging moving samples without stopping,the system achieves high-speed 3D image acquisition of an entire mouse brain within1.5 hours,at a resolution capable of visualizing synaptic spines.A pipeline is developed for sample preparation,imaging,3D image reconstruction and quantification.Our approach is compatible with immunofluorescence methods,enabling flexible cell-type specific brain mapping and is readily scalable for large biological samples such as primate brains.Using this system,we examined behaviorally relevant whole-brain neuronal activation in 16 c-Fos-sh EGFP mice under resting or forced swimming conditions.Our results indicate the involvement of multiple subcortical areas in stress response.Intriguingly,neuronal activation in these areas exhibits striking individual variability among different animals,suggesting the necessity of sufficient cohort size for such studies. | Hao Wang Qingyuan Zhu Lufeng Ding Yan Shen Chao-Yu Yang Fang Xu Chang Shu Yujie Guo Zhiwei Xiong Qinghong Shan Fan Jia Peng Su Qian-Ru Yang Bing Li Yuxiao Cheng Xiaobin He Xi Chen Feng Wu Jiang-Ning Zhou Fuqiang Xu Hua Han Pak-Ming Lau Guo-Qiang Bi | 2019 | National Science Review2019，6，5： | 7 |
| 2 | Experimental study of photooxidation products of ethylbenzene显示文摘Smog chamber experiments were performed to investigate the composition of products formed from photooxidation of aromatic hydrocarbon ethylbenzene.Vacuum ultraviolet photoionization mass spectrometer and aerosol time-of-flight mass spectrometer were used to measure the products in the gas and particle phases in real-time.Experimental results demonstrated that ethylphenol,methylglyoxal,phenol,benzaldehyde,and 2-ethylfurane were the predominant photooxidation products in both the gas and particle phases.However,there were some dierences between detected gas phase products and those of particle phase,for example,2-ethylfurane,ethylglyoxylic acid,nitroethylbenzene,3,4-dioxopentanal and ethyl-nitrophenol were only existing in the particle-phase. The possible reaction mechanisms leading to these products were also discussed and proposed. | Mingqiang Huang Weijun Zhang Liqing Hao Zhenya Wang Li Fang Ruihong Kong Xiaobin Shan Fuyi Liu Liusi Sheng | 2010 | Journal of Environmental Sciences2010，22，10： | 5 |
| 3 | Monitoring of atmospheric radionuclides from the Fukushima nuclear accident and assessing their impact on Xi'an, China显示文摘Aerosol radionuclides (131I, 134Cs, 137Cs) and gaseous radioactive xenon (133Xe) were monitored at Xi'an, China following the accident at the Fukushima nuclear power plant in March 2011. The additional annual effective dose attributable to the Fukushima emissions was much lower than the public annual effective dose from natural radiation, according to Chinese national standards. The monitoring results were compared with data from other countries as well as with the radionuclide concentrations observed in Xi'an after the Chernobyl nuclear accident in 1986. Possible transport pathways of the released radionuclides from Fukushima to Xi'an were investigated. The occurrence of an anticyclone in the Pacific Ocean region and the extended period over which the radionuclides were released made the determination transport pathways complex, but divergence in the plume and easterly flow evidently brought the initial suite of radionuclides to Xi'an. | LIU LongBo WU Shan CAO JunJi XIE Feng SHI QuanLin ZHANG ChangYun TANG HanBing HE XiaoBin ZHANG Rong CHEN LiYun WEI GuanYi ZHANG ZhiHong ZHANG JiaMei DANG HaiJun | 2013 | Chinese Science Bulletin2013，58，13： | 4 |
| 4 | Carbon emissions induced by farmland expansion in China during the past 300 years显示文摘Scientific assessment of the accounting over carbon in the terrestrial ecosystem in the process land use/land cover changes caused by human activities will help reduce the uncertainty in estimating carbon emissions from the terrestrial ecosystem. This study employs a bookkeeping model to estimate the carbon emissions from farmland reclamation in China during the past 300 years based on the annual rate of land use changes(derived from historical natural vegetation, farmland data), preset carbon density and coefficients of disturbance curves. We find out that:(1) there was a net increase of 79.30×10~4km^2 in national farmland; about 65% of reclaimed farmland had been forest land and 26% of that had been grass land previously;(2) the total amount of carbon emissions from farmland expansion in China had been between 2.94 and 5.61 Pg with the median 3.78 Pg during the past 300 years; specifically, carbon emissions of vegetation were 1.58 Pg while those of soil ranged from 1.35 Pg to4.03 Pg with the median 2.20 Pg;(3) carbon emissions vary greatly across various ecosystems: the emissions were most from forest land, and then grass land and swamps, and the least from shrubs; deserts functioned more likely to be carbon stock in the process of land reclamation;(4) along the time line, carbon emissions had decreased first and then increased while the peak emissions occurred in the first half of 20 th century; and spatially, carbon emissions were most released in Northeast and Southwest China; Northwest China was of the minimum carbon emissions. | Xuhong YANG Xiaobin JIN Xiaomin XIANG Yeting FAN Jing LIU Wei SHAN Yinkang ZHOU | 2019 | Science China Earth Sciences2019，62，2： | 2 |
| 5 | The muscle-specific microRNAs miR-1 and miR-133 produce opposing effects on apoptosis by targeting HSP60, HSP70 and caspase-9 in cardiomyocytes显示文摘 | Chaoqian Xu Yanjie Lu Zhenwei Pan Wenfeng Chu Xiaobin Luo Huixian Lin Jiening Xiao Hongli Shan Zhiguo Wang and Baofeng Yang | | Journal of Cell Science0，120，17： | 1 |
| 6 | A VUV photoionization mass spectrometric study on the OH-initiated photooxidation of isoprene with synchrotron radiation显示文摘The gas-phase organic compounds resulting from OH-initiated photooxidation of isoprene have been investigated on-line by VUV photoionization mass spectrometry based on synchrotron radiation for the first time.The photoionization efficiency curves of the corresponding gaseous products as well as the chosen standards have been deduced by gating the interested peaks in the photoionization mass spectra while scanning the photon energy simultaneously,which permits the identification of the pivotal gaseous products of the photooxidation of isoprene,such as formaldehyde(10.84eV),formic acid(11.38eV),acetone(9.68eV),glyoxal(9.84eV),acetic acid(10.75eV),methacrolein(9.91eV),and methyl vinyl ketone(9.66eV).Proposed reaction mechanisms leading to the formation of these key products were discussed,which were completely consistent with the previous works of different groups.The capability of synchrotron radiation photoionization mass spectrometry to directly identify the chemical composition of the gaseous products in a simulation chamber has been demonstrated,and its potential application in related studies of atmospheric oxidation of ambient volatile organic compounds is anticipated. | Gang Pan Changjin Hu Mingqiang Huang Zhenya Wang Yue Cheng Zhi Liu Weixiong Zhao Weijun Zhang Jun Chen Fuyi Liu Xiaobin Shan Liusi Sheng | 2012 | Journal of Environmental Sciences2012，24，12： | 1 |
| 7 | Construction and Immunogenicity of Recombinant Lactococcus lactis Expressing S1 Protein of Porcine Epidemic Diarrhea Virus(PEDV)显示文摘To evaluate the specific immune responses induced by recombinant Lactococcus lactis(L.lactis) which expresses porcine epidemic diarrhea virus(PEDV) S1 protein through oral administration,the spike gene fragment of PEDV was amplified from PEDV SDLY strain to construct p MG36 e-S1 recombinant plasmid.The recombinant plasmid was then electro-transferred into competent cells of L.lactis MG1363,to prepare the recombinant L.lactis expressing S1 protein of PEDV.The expression of target protein was identified by SDS-PAGE and Western-blot.New Zealand white rabbits were orally administered with the recombinant strain;the antibody titer in intestinal mucosa and serum was detected by neutralizing test;and the specific Ig G in serum was evaluated by indirect ELISA.The results showed that the recombinant L.lactis could effectively induce high level of Ig G in serum and high level of mucosal immune antibody.The recombinant L.lactis is qualified to be a potential oral vaccine because it could successfully stimulate both humoral and mucosal immune responses against PEDV. | Wang Liping Han Xianjie Wang Xiaobin Gai Chunyun Li Junwei Shan Hu | 2018 | Animal Husbandry and Feed Science2018，10，2： | 1 |
| 8 | Antarcticite, a Phase Change Material for ThermalEnergy Storage: Experiments and Simulation显示文摘Antarcticite, a mineral with composition CaCl2·6H2O and structure P321, is an ideal phase change material (PCM) due to the high energy storage density and good thermal conductivity. However, the existence of supercooling and incongruent melting would weaken its thermal properties and then hinder its application. In this paper, based on the cooling curve method and DSC measurement, we experimentally selected the minor SrCl2·6H2O as the nucleator and carboxyl methyl cellulose as the thickening agent, which could significantly reduce supercooling and partly restrain the incongruent melting. Moreover, we incorporated Antarcticite as PCM into building envelopes in four different cases, the simulation of the heat transfer processes showed that the temperature fluctuation could be reduced to about 2℃ in the best case. | GU Xiaobin NIU Jingjing QIN Shan | 2013 | 矿物学报2013，33，S1： | 1 |
| 9 | A novel inhibitor of ARfl and ARv7 induces protein degradation to overcome enzalutamide resistance in advanced prostate cancer显示文摘Enzalutamide(ENZ) is a second-generation androgen receptor(AR) antagonist used for the treatment of castration-resistant prostate cancer(CRPC) and reportedly prolongs survival time within a year of starting therapy. However, CRPC patients can develop ENZ resistance(ENZR), mainly driven by abnormal reactivation of AR signaling, involving increased expression of the full-length AR(ARfl)or dominantly active androgen receptor splice variant 7(ARv7) and ARfl/ARv7 heterodimers. There is currently no efficient treatment for ENZR in CRPC. Herein, a small molecule LLU-206 was rationally designed based on the ENZ structure and exhibited potent inhibition of both ARfl and constitutively active ARv7 to inhibit PCa proliferation and suppress ENZR in CRPC. Mechanically, LLU-206 promoted ARfl/ARv7 protein degradation and decreased ARfl/ARv7 heterodimers through mouse double minute 2-mediated ubiquitination. Finally, LLU-206 exhibited favorable pharmacokinetic properties with poor permeability across the blood-brain barrier, leading to a lower prevalence of adverse effects, including seizure and neurotoxicity, than ENZ-based therapies. In a nutshell, our findings demonstrated that LLU-206 could effectively inhibit ARfl/ARv7-driven CRPC by dual-targeting of ARfl/ARv7 heterodimers and protein degradation, providing new insights for the design of new-generation AR inhibitors to overcome ARfl/ARv7-driven CRPC. | Yan Li Ya Chu Guangjiang Shi Xiaobin Wang Wanli Ye Chun Shan Dajia Wang Di Zhang Wei He Jingwei Jiang Shuqian Ma Yuhong Han Zhili Zhao Shijia Du Zhen Chen Zhiyu Li Yong Yang Chen Wang Xi Xu Hongxi Wu | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 0 |
| 10 | Soft X-ray in-line holographic experiment显示文摘Water window' soft X-ray has some advantages in the microimaging of biological specimens. Soft X-ray holography can give us three-dimensional images of biological cells in their natural condition. Quasi-monochromatic soft X-ray from the polymonochromatic synchrotron radiation is obtained by a zone plate-pinhole linear monochromator at National Synchrotron Radiation Laboratory (NSRL) at Hefei. The pinhole is used to increase the temporal coherence of the soft X-ray, and also to increase the spatial coherence of it. An in-line holographic experiment has been finished with soft X-ray of main wavelength 4.5 nm and pinhole of diameter 0.03 mm. The magnified hologram is reconstructed with He-Ne laser. A smaller pinhole is wanted to be used to improve the coherence of the soft X-ray so as to improve the quality of the hologram. | Shaoguang Yang Xingshu Xie Chengzhi Jia Yongfei Zhao Shiping Jiang Yuxuan Zhang Xiaobin Shan Xinyi Zhang | 1998 | Chinese Science Bulletin1998，43，8： | 0 |
| 11 | Single-cell landscape analysis reveals distinct regression trajectories and novel prognostic biomarkers in primary neuroblastoma显示文摘Neuroblastoma(NB),which is the most common pediatric extracranial solid tumor,varies widely in its clinical presentation and outcome.NB has a unique ability to spontaneously differentiate and regress,suggesting a potential direction for therapeutic intervention.However,the underlying mechanisms of regression remain largely unknown,and more reliable prognostic biomarkers are needed for predicting trajectories for NB.We performed scRNAseq analysis on 17 NB clinical samples and three peritumoral adrenal tissues.Primary NB displayed varied cell constitution,even among tumors of the same pathological subtype.Copy number variation patterns suggested that neuroendocrine cells represent the malignant cell type.Based on the differential expression of sets of related marker genes,a subgroup of neuroendocrine cells was identified and projected to differentiate into a subcluster of benign fibroblasts with highly expressed CCL2 and ZFP36,supporting a progressive pathway of spontaneous NB regression.We also identified prognostic markers(STMN2,TUBA1A,PAGE5,and ETV1)by evaluating intra-tumoral heterogeneity.Lastly,we determined that ITGB1 in M2-like macrophages was associated with favorable prognosis and may serve as a potential diagnostic marker and therapeutic target.In conclusion,our findings reveal novel mechanisms underlying regression and potential prognostic markers and therapeutic targets of NB. | Qingqing Liu Zhenni Wang Yan Jiang Fengling Shao Yue Ma Mingzhao Zhu Qing Luo Yang Bi Lijian Cao Liang Peng Jianwu Zhou Zhenzhen Zhao Xiaobin Deng Tong-Chuan He Shan Wang | 2022 | Genes & Diseases2022，9，6： | 0 |
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