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| 1 | Single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to 2019-nCoV infection显示文摘It has been known that,the novel coronavirus,2019-nCoV,which is considered similar to SARS-CoV,invades human cells via the receptor angiotensin converting enzyme II(ACE2).Moreover,lung cells that have ACE2 expression may be the main target cells during 2019-nCoV infection.However,some patients also exhibit non-respiratory symptoms,such as kidney failure,implying that 2019-nCoV could also invade other organs.To construct a risk map of different human organs,we analyzed the single-cell RNA sequencing(scRNA-seq)datasets derived from major human physiological systems,including the respiratory,cardiovascular,digestive,and urinary systems.Through scRNA-seq data analyses,we identified the organs at risk,such as lung,heart,esophagus,kidney,bladder,and ileum,and located specific cell types(i.e.,type II alveolar cells(AT2),myocardial cells,proximal tubule cells of the kidney,ileum and esophagus epithelial cells,and bladder urothelial cells),which are vulnerable to 2019-nCoV infection.Based on the findings,we constructed a risk map indicating the vulnerability of different organs to 2019-nCoV infection.This study may provide potential clues for further investigation of the pathogenesis and route of 2019-nCoV infection. | Xin Zou Ke Chen Jiawei Zou Peiyi Han Jie Hao Zeguang Han | 2020 | Frontiers of Medicine2020，14，2： | 193 |
| 2 | Increased frequency and clinical significance of myeloidderived suppressor cells in human colorectal carcinoma显示文摘AIM:To investigate the frequency and clinical significance of the myeloid-derived suppressor cells(MDSC) in human colorectal carcinoma(CRC).METHODS:Samples of peripheral blood and tumor tissue from 49 CRC patients were analyzed.Mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation and were subjected to a flow cytometrybased immunophenotypic analysis.RESULTS:A considerable increase in the percentage of CD33+HLA-DR-MDSCs was observed in the peripheral blood(1.89% ± 0.75%) and tumor tissues(2.99% ± 1.29%) of CRC patients as compared with that in theperipheral blood of healthy controls(0.54% ± 0.35%).This expanded CD33+HLA-DR-subset exhibited immature myeloid cell markers,but not lineage markers,and showed up-regulation of CD18/CD11b expression as compared with the MDSCs from healthy donors.Further studies showed that the MDSC proportion in CRC peripheral blood was correlated with nodal metastasis(P = 0.023),whereas that in tumor tissues was correlated with nodal/distant metastasis(P = 0.016/P = 0.047) and tumor stage(P = 0.028),suggesting the involvement of MDSCs in CRC tumor development.CONCLUSION:Characterization of MDSCs in CRC suggests the clinical significance of circulating and tumorinfiltrating MDSCs and may provide new insights into the CRC immunotherapy targeting MDSCs. | Hong-Li Sun, Xin Zhou, Yi-Feng Xue Ke Wang, Yun-Feng ShenI Jing-Jue Mao Hong-Feng Guo Zong-Ning Miao | 2012 | World Journal of Gastroenterology2012，18，25： | 21 |
| 3 | Overexpression of p27^(KIP1)induced cell cycle arrest in G_1 phase and subsequent apoptosis in HCC-9204 cell line显示文摘AIM We have previously reported that inducibleover-expression of Bak may prolong cell cycle inG1 phase and lead to apoptosis in HCC-9204 cells.This study is to investigate whether p27KIP1playsan important role in this process.METHODS In order to elucidate the exactfunction of p27KIP1in this process,a zinc induciblep27KIP1stable transfectant and transient p27KIP1-GFP fusion transfectant were constructed.Theeffects of inducible,p27KIP1on cell growth,cellcycle arrest and apoptosis were examined in themock,control pMD vector,and pMD-KIP1transfected HCC-9204 cells.RESULTS This p27KIP1-GFP transfectant maytransiently express the fusion gene.The cellgrowth was reduced by 35% at 48 h of p27KIP1induction with zinc treatment as determined bytrypan blue exclusion assay.These differencesremained the same after 72 h of p27KIP1expression,p27KIP1caused cell cycle arrest after24 h of induction,with 40% increase in G1population.Prolonged p27KIP1expression in thiscell line induced apoptotic cell death reflected byTUNEL assay.Fourty-eight h and 72 h of p27KIP1expression showed a characteristic DNA ladder onagarose gel electrophoresis. CONCLUSION Bak may induce cell cycle arrest inG1 phase through upregulating expression ofp27KIP1and subsequently lead to apoptosis inHCC-9204 cells.The p27KIP1-GFP fusion proteincan be transiently expressed in HCC-9204 cells.The inducible p27KIP1-expressing cell line providesa model to assess p27KIP1function. | Jiang Li Xin Ke Yang Xin Xin Yu Meng Liang Ge Wen Liang Wang Jie Zhang Yun De Hou Department of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China State Key Laboratory for Molecular Virology and Genetic Engineering,Beijing 100052,China Department of Dermatology,Beijing Hospital,Beijing 100016,China Institute of Radiation Medicine,Beijing 100085,China | 2000 | World Journal of Gastroenterology2000，6，4： | 20 |
| 4 | ROS activates JNK-mediated autophagy to counteract apoptosis in mouse mesenchymal stem cells in vitro显示文摘目的: 为糖尿病的可勃起的机能障碍(编辑) 的治疗的间充质的干细胞(MSC ) 的移植被移植房间的 apoptosis 妨碍。在糖尿病的编辑,有增加的氧化应力并且在阴茎海绵体,和反应的氧种类(ROS ) 不减少导致移植房间的 apoptosis。在这研究,我们检验了是否并且 autophagy 怎么涉及 MSCs.Methods 的导致 ROS 的 apoptosis : 老鼠 C3H10 MSC 与 H 2 在糖尿病的编辑房间生存能力模仿高氧化的条件的 O 2 用 MTT 被测量试金。Apoptosis 被流动 cytometry 分析。Apoptosis 相关、 autophagy 相关的蛋白质与西方的污点试金被检测。细胞内部的 autophagosome 累积用传播电子 microscopy.Results 被学习: 有 H 2 O 2(50-400 μ mol/L ) 在集中依赖者和时间依赖者礼貌禁止了房间生存能力。而且, H 2 O 2(300 μ mol/L ) 在 MSC 的导致的 apoptosis,以及激活的 autophagy。有 lysosome 禁止者 chloroquine 的预告的处理(10 μ mol/L ) 或 PI3K 禁止者 3-methyladenine (5 mmol/L ) 显著地提高了 H 2 O 2-induced 房间死亡。有 JNK 禁止者 SP600125 的预告的处理(10 μ mol/L ) 废除 H 2 LC3-II 的 O 2-induced 累积,和稀释 H 2 在 MSCs.Conclusion 的 Bcl-2 层次的 O 2-induced 减小: ROS 导致 autophagy 由 JNK 的激活在 MSC 抵抗 apoptosis。因此, autophagy 的扩大可以减少 apoptosis,延长 MSC 幸存并且改进为糖尿病的编辑的基于 MSC 的治疗学的功效。 | Guan-yu LIU Xiao-xue JIANG Xin ZHU Wei-yang HE You-lin KUANG Ke REN Yong LIN Xin GOU | 2015 | Acta Pharmacologica Sinica2015，36，12： | 20 |
| 5 | Coinfection with influenza A virus enhances SARS-CoV-2 infectivity显示文摘The upcoming flu season in the Northern Hemisphere merging with the current COVID-19 pandemic raises a potentially severe threat to public health.Through experimental coinfection with influenza A virus(IAV)and either pseudotyped or live SARS-CoV-2 virus,we found that IAV preinfection significantly promoted the infectivity of SARS-CoV-2 in a broad range of cell types.Remarkably,in vivo,increased SARS-CoV-2 viral load and more severe lung damage were observed in mice coinfected with IAV.Moreover,such enhancement of SARS-CoV-2 infectivity was not observed with several other respiratory viruses,likely due to a unique feature of IAV to elevate ACE2 expression.This study illustrates that IAV has a unique ability to aggravate SARS-CoV-2 infection,and thus,prevention of IAV infection is of great significance during the COVID-19 pandemic. | Lei Bai Yongliang Zhao Jiazhen Dong Simeng Liang Ming Guo Xinjin Liu Xin Wang Zhixiang Huang Xiaoyi Sun Zhen Zhang Lianghui Dong Qianyun Liu Yucheng Zheng Danping Niu Min Xiang Kun Song Jiajie Ye Wenchao Zheng Zhidong Tang Mingliang Tang Yu Zhou Chao Shen Li Zhou Yu Chen Huan Yan Ke Lan Ke Xu | 2021 | Cell Research2021，31，4： | 16 |
| 6 | Laparoscopic vs computerized tomography-guided radiofrequency ablation for large hepatic hemangiomas abutting the diaphragm显示文摘AIM: To compare safety and therapeutic efficacy of laparoscopic radiofrequency(RF) ablation vs computed tomography(CT)-guided RF ablation for large hepatic hemangiomas abutting the diaphragm.METHODS: We retrospectively reviewed our sequential experience of treating 51 large hepatic hemangiomas abutting the diaphragm in 51 patients by CT-guided or laparoscopic RF ablation due to either the presence of symptoms and/or the enlargement of hemangioma.Altogether, 24 hemangiomas were ablated via a CTguided percutaneous approach(CT-guided ablation group), and 27 hemangiomas were treated via a laparoscopic approach(laparoscopic ablation group).RESULTS: The mean diameter of the 51 hemangiomas was 9.6 ± 1.8 cm(range, 6.0-12.0 cm). There was nodifference in the diameter of hemangiomas between the two groups(P > 0.05). RF ablation was performed successfully in all patients. There was no difference in ablation times between groups(P > 0.05). There were 23 thoracic complications in 17 patients: 15(62.5%, 15/24) in the CT-guided ablation group and2(7.4%, 2/27) in the laparoscopic ablation group(P< 0.05). According to the Dindo-Clavien classification,two complications(pleural effusion and diaphragmatic rupture grade Ⅲ) were major in two patients. All others were minor(grade Ⅰ). Both major complications occurred in the CT-guided ablation group. The minor complications were treated successfully with conservative measures, and the two major complications underwent treatment by chest tube drainage and thoracoscopic surgery, respectively. Complete ablation was achieved in 91.7%(22/24) and 96.3%(26/27) in the CT-guided and the laparoscopic ablation groups,respectively(P > 0.05).CONCLUSION: Laparoscopic RF ablation therapy should be used as the first-line treatment option for large hepatic hemangiomas abutting the diaphragm.It avoids thermal injury to the diaphragm and reduces thoracic complications. | Jun Gao Jian Kong Xue-Mei Ding Shan Ke Hai-Gang Niu Zong-Hai Xin Chun-Min Ning Shi-Gang Guo Xiao-Long Li Long Zhang Yong-Hong Dong Wen-Bing Sun | 2015 | World Journal of Gastroenterology2015，21，19： | 16 |
| 7 | Calcium channel blockers reduce severe fever with thrombocytopenia syndrome virus (SFTSV) related fatality显示文摘Severe fever with thrombocytopenia syndrome (SFTS),an emerging tick-borne infectious disease caused by a novel phlebovirus (SFTS virus, SFTSV), was listed among the top 10 priority infectious diseases by the World Health Organization due to its high fatality of 12%-50% and possibility of pandemic transmission. Currently, effective anti-SFTSV intervention remains unavailable. Here, by screening a library of FDA-approved drugs, we found that benidipine hydrochloride, a calcium channel blocker (CCB), inhibited SFTSV replication in vitro. Benidipine hydrochloride was revealed to inhibit virus infection through impairing virus internalization and genome replication. Further experiments showed that a broad panel of CCBs, including nifedipine, inhibited SFTSV infection. The anti-SFTSV effect of these two CCBs was further an a lyzed in a humanized mouse model in which CCB treatment resulted in reduced viral load and decreased fatality rate. Importantly, by performing a retrospective clinical investigation on a large cohort of 2087 SFTS patients, we revealed that nifedipine administration enhaneed virus clearance, improved clinical recovery, and remarkably reduced the case fatality rate by >5-fold. These findings are highly valuable for developing potential host-oriented therapeutics for SFTS and other lethal acute viral infections known to be inhibited by CCBs in vitro. | Hao Li Lei-Ke Zhang Shu-Fen Li Shao-Fei Zhang Wei-Wei Wan Yu-Lan Zhang Qi-Lin Xin Ke Dai Yuan-Yuan Hu Zhi-Bo Wang Xiang-Tao Zhu Yu-Jie Fang Ning Cui Pan-He Zhang Chun Yuan Qing-Bin Lu Jie-Ying Bai Fei Deng Geng-Fu Xiao Wei Liu Ke Peng | 2019 | Cell Research2019，29，9： | 15 |
| 8 | Radiofrequency ablation for hepatic hemangiomas: A consensus from a Chinese panel of experts显示文摘Recent studies have shown that radiofrequency(RF) ablation therapy is a safe, feasible, and effective procedure for hepatic hemangiomas, even huge hepatic hemangiomas. RF ablation has the following advantages in the treatment of hepatic hemangiomas: minimal invasiveness, definite efficacy, high safety, fast recovery, relatively simple operation, and wide applicability. It is necessary to formulate a widely accepted consensus among the experts in China who have extensive expertise and experience in the treatment of hepatic hemangiomas using RF ablation, which is important to standardize the application of RF ablation for the management of hepatic hemangiomas, regarding the selection of patients with suitable indications to receive RF ablation treatment, the technical details of the techniques, therapeutic effect evaluations, management of complications, etc. A final consensus by a Chinese panel of experts who have the expertise of using RF ablation to treat hepatic hemangiomas was reached by means of literature review, comprehensive discussion, and draft approval. | Jun Gao Rui-Fang Fan Jia-Yin Yang Yan Cui Jian-Song Ji Kuan-Sheng Ma Xiao-Long Li Long Zhang Chong-Liang Xu Xin-Liang Kong Shan Ke Xue-Mei Ding Shao-Hong Wang Meng-Meng Yang Jin-Jin Song Bo Zhai Chun-Ming Nin Shi-Gang Guo Zong-Hai Xin Jun Lu Yong-Hong Dong Hua-Qiang Zhu Wen-Bing Sun | 2017 | World Journal of Gastroenterology2017，23，39： | 15 |
| 9 | Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China显示文摘Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus(MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing(MLST), spa typing, multiple-locus variable number of tandem repeat analysis(MLVA), pulsed-field gel electrophoresis(PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance(MDR). Greater diversity was found in food-associated MRSA(7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA(3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates(40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone(96.4%), and CC9-ST9-t437-MC621(20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains(t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China. | WANG Wei LIU Feng ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin | 2017 | Biomedical and Environmental Sciences2017，30，8： | 13 |
| 10 | Meta-analysis of cognitive function in Chinese first-episode schizophrenia: MATRICS Consensus Cognitive Battery (MCCB) profile of impairment显示文摘Background Compromised neurocognition is a core feature of schizophrenia. With increasing studies researching cognitive function of Chinese patients with first-episode schizophrenia (FES) using MATRICS Consensus Cognitive Battery (MCCB), it is not clear about the level and pattern of cognitive impairment among this population. Aim To provide a meta-analysis systematically analysing studies of neurocognitive function using MCCB in Chinese patients with FES. Methods An independent literature search of both Chinese and English databases up to 13 March 2019 was conducted by two reviewers. Standardised mean difference (SMD) was calculated using the random effects model to evaluate the effect size. Results 56 studies (FES=3167, healthy controls (HC)=3017) were included and analysed. No study was rated as 'high quality' according to Strengthening the Reporting of Observational Studies in Epidemiology. Compared with HCs, Chinese patients with FES showed impairment with large effect size in overall cognition (SMD=-1.60,95% Cl -1.82 to -1.38,厂=67%) and all seven cognitive domains, with the SMD ranging from -0.87 to -1.41. In nine MCCB subtests, patients with FES showed significant difference in Symbol Coding (SMD=-1.90), Trail Making Test (TMT)(SMD=-1.36), Continuous Performance Test-Identical Pairs (SMD=-1.33), Hopkins Verbal Learning Test (SMD=-1.24), Brief Visuospatial Memory Test (SMD=-1.18), Mazes (SMD=-1.16), Category Fluency (SMD=-1.01), Spatial Span (SMD=-0.69) and Mayer-Salovey-Caruso Emotional Intelligence Test (SMD=-0.38). Conclusions Our meta-analysis demonstrates that Chinese patients with FES show neurocognitive deficits across all seven MCCB cognitive domains and all nine subtests, particularly in two neurocognitive domains: speed of processing and attention/vigilance, with the least impairment shown in social cognition. Symbol Coding and TMT may be the most sensitive tests to detect cognitive deficit in Chinese patients with FES. | Huijuan Zhang Yao Wang Yuliang Hu Yikang Zhu Tian hong Zhang Jijun Wang Ke Ma Chuan Shi Xin Yu Chunbo Li | 2019 | General Psychiatry2019，32，3： | 13 |
| 11 | Removal of heavy metals from a contaminated soilusing tartaric acid显示文摘This study reports the feasibility of remediation of a heavy metal (HM ) contaminated soil using tartaric acid, an environmentally-friendly extractant. Batch experiments were performed to test the factors influencing remediation of the HM contaminated soil. An empirical model was employed to describe the kinet ics of HM dissolution/desorption and to predict equilibrium concentrations of HM s in soil leachate. The changes of HMs in different fractions before and after t artaric acid treatment were also investigated. Tartaric acid solution containing HMs was regenerated by chestnut shells. Results show that utilization of tartar ic acid was effective for removal of HMs from the contaminated soil, attaining 5 0%—60% of Cd, 40%—50% of Pb, 40%—50% of Cu and 20%—30% of Zn in the pH ra nge of 3.5—4.0 within 24 h. Mass transfer coefficients for cadmium (Cd) and lead (Pb) were much higher than those for copper (Cu) and zinc (Zn). Sequ ential fractionations of treated and untreated soil samples showed that tartari c acid was effective in removing the exchangeable, carbonate fractions of Cd, Zn and Cu from the contaminated soil. The contents of Pb and Cu in Fe-Mn oxide fra citon were also significantly decreased by tartaric acid treatment. One hundred milliliters of tartaric acid solution containing HMs could be regenerated by 10 g chestnut shells in a batch reactor. Such a remediation procedure indicated tha t tartaric acid is a promising agent for remediation of HM contaminated soils. H owever, further research is needed before the method can be practically used for in situ remediation of contaminated sites. | KE Xin LI Pei-jun ZHOU Qi-xing ZHANG Yun SUN Tie-heng | 2006 | Journal of Environmental Sciences2006，18，4： | 13 |
| 12 | Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats显示文摘 | Wen-ya HUANG Xin ZOU Fu-er LU Hao SU Chu ZHANG Yan-lin REN Ke FANG Li-jun XU Kai- fu WANG Qing-jie CHEN Hui DONG | 2018 | Current Medical Science2018，38，4： | 13 |
| 13 | Low-temperature processed inorganic hole transport layer for efficient and stable mixed Pb-Sn low-bandgap perovskite solar cells显示文摘Metal halide perovskite solar cells(PSCs)have attracted tremendous attention as an emerging photovoltaic technology due to their high efficiency,low cost and ease of fabrication from earth-abundant materials[1,2].The power conversion efficiencies(PCEs)have been rapidly boosted from 3.8%in the pioneer’s work to a certified 24.2%nowadays in just ten years[3].The PCE breakthroughs in PSCs have mostly adopted full lead-based perovskites(APbX3)with bandgaps of 1.5–1.6 eV. | Qiaolei Han Ying Wei Renxing Lin Zhimin Fang Ke Xiao Xin Luo Shuai Gu Jia Zhu Liming Ding Hairen Tan | 2019 | Science Bulletin2019，64，19： | 13 |
| 14 | Molecular Typing of Leptospira interrogans Strains Isolated from Rattus tanezumi in Guizhou Province,Southwest of China显示文摘Objective To identify and type three leptospires isolated from Rattus tanezumi in Guizhou Province by using three molecular techniques (PFGE, MLVA, and MLST), reveal the molecular characteristic of causative agents of local leptospirosis and evaluate these three molecular methods based on their detection resolution and efficiency. Methods Three Leptospira strains were isolated from the kidney of Rattus tanezumi and cultured with EMJH medium. PFGE, MLVA, and MLST assays were applied to type the three strains isolated from Rattus tanezumi in Guizhou Province. Results PFGE, MLVA, and MLST typing showed that the three leptospiral isolates matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai. The findings of the genotyping methods were consistent. MLVA and MLST defined genotypes, whereas PFGE allowed the recognition of additional subgroups within the genotypes, and the findings of molecular typing were also consistent with those of traditional techniques. Conclusion Three leptospiral isolates from Guizhou Province matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai, and PFGE, MLVA, and MLST, as reliable molecular techniques for identifying and typing of Leptospira interrogans, would contribute to the active surveillance, outbreak investigation and source tracking for leptospirosis in Guizhou Province. | LI Shi Jun ZHANG Cui Cai LI Xiu Wen TIAN Ke Cheng TANG Guang Peng WANG Ding Ming LIU Ying NIE Yi Xin JIANG Xiu Gao | 2012 | Biomedical and Environmental Sciences2012，25，5： | 12 |
| 15 | Direct low-temperature synthesis of graphene on various glasses by plasma-enhanced chemical vapor deposition for versatile, cost-effective electrodes显示文摘在低温度的各种各样的玻璃底层上的 graphene 的没有催化剂、可伸缩的合成具有到象便宜透明电子学和最先进的显示器那样的众多的应用程序的首要的意义。然而,在这个有希望的研究领域以内的系统的学习远这样仍然保持少见。此处,我们用一个低温度的提高血浆的化学蒸汽免职方法在各种各样的眼镜上报导 graphene 的直接生长。如此的一条灵巧、可伸缩的途径在 400600 的一个生长温度范围在各种各样的玻璃底层上保证一致、没有转移的 graphene | Jingyu Sun Yubin Chen Xin Cai Bangjun Ma Zhaolong Chen Manish Kr. Priydarshi Ke Chen Teng Gao Xiuju Song Qingqing Ji Xuefeng Guo Dechun Zou Yanfeng Zhang Zhongfan Liu | 2015 | Nano Research2015，8，11： | 12 |
| 16 | Rapid and Accurate Sequencing of Enterovirus Genomes Using MinION Nanopore Sequencer显示文摘Objective Knowledge of an enterovirus genome sequence is very important in epidemiological investigation to identify transmission patterns and ascertain the extent of an outbreak.The MinION sequencer is increasingly used to sequence various viral pathogens in many clinical situations because of its long reads,portability,real-time accessibility of sequenced data,and very low initial costs.However,information is lacking on MinION sequencing of enterovirus genomes.Methods In this proof-of-concept study using Enterovirus 71(EV71) and Coxsackievirus A16(CA16) strains as examples,we established an amplicon-based whole genome sequencing method using MinION.We explored the accuracy,minimum sequencing time,discrimination and high-throughput sequencing ability of MinION,and compared its performance with Sanger sequencing.Results Within the first minute(min) of sequencing,the accuracy of MinION was 98.5% for the single EV71 strain and 94.12%-97.33% for 10 genetically-related CA16 strains.In as little as 14 min,99% identity was reached for the single EV71 strain,and in 17 min(on average),99% identity was achieved for 10 CA16 strains in a single run.Conclusion MinION is suitable for whole genome sequencing of enteroviruses with sufficient accuracy and fine discrimination and has the potential as a fast,reliable and convenient method for routine use. | WANG Ji KE Yue Hua ZHANG Yong HUANG Ke Qiang WANG Lei SHEN Xin Xin DONG Xiao Ping XU Wen Bo MA Xue Jun | 2017 | Biomedical and Environmental Sciences2017，30，10： | 11 |
| 17 | In Situ Corrosion Monitoring of Mild Steel in a Simulated Tidal Zone without Marine Fouling Attachment by Electrochemical Impedance Spectroscopy显示文摘The corrosion behaviors of the isolated short and vertical long scale Q235 B steel in a simulated tidal zone were studied by electrochemical impedance spectroscopy(EIS) monitoring and corrosion weight loss calculation in an experimental indoor simulating trough.The results show that the corrosion rate of the isolated short scale Q235 B steel in the tidal zone acquired by the EIS agrees with the corrosion weight loss result.The corrosion rates of the short scale steel are in the order of middle tidal zone > the central zone between the middle tidal zone and low tidal zone > high tidal zone > low tidal zone.The fastest corrosion rate in the middle tidal zone is attributed to the longest wet time in a tidal cycle.According to the comparison of corrosion weight loss between the vertical long scale and isolated short scale specimens,the corrosion rate of vertical long scale specimens of Q235 B steel is lower than that of the isolated short scale specimens in the tidal zone,but the result is contrary in the immersion zone. | Xin Mu Jie Wei Junhua Dong Wei Ke | 2014 | Journal of Materials Science & Technology2014，30，10： | 11 |
| 18 | Ultrafast fiber lasers mode-locked by two-dimensional materials:review and prospect显示文摘The year 2019 marks the 10th anniversary of the first report of ultrafast fiber laser mode-locked by graphene.This result has had an important impact on ultrafast laser optics and continues to offer new horizons.Herein,we mainly review the linear and nonlinear photonic properties of two-dimensional(2D)materials,as well as their nonlinear applications in efficient passive mode-locking devices and ultrafast fiber lasers.Initial works and significant progress in this field,as well as new insights and challenges of 2D materials for ultrafast fiber lasers,are reviewed and analyzed. | TIAN JIANG KE YIN CONG WANG JIE YOU HAO OUYANG RUNLIN MIAO CHENXI ZHANG KE WEI HAN LI HAITAO CHEN RENYAN ZHANG XIN ZHENG ZHONGJIE XU XIANGAI CHENG HAN ZHANG | 2020 | Photonics Research2020，8，1： | 11 |
| 19 | Metabonomic analysis of hepatitis B virus-induced liver failure:identification of potential diagnostic biomarkers by fuzzy support vector machine显示文摘Hepatitis B virus(HBV)-induced liver failure is an emergent liver disease leading to high mortality.The severity of liver failure may be reflected by the profile of some metabolites.This study assessed the potential of using metabolites as bio- markers for liver failure by identifying metabolites with good discriminative performance for its phenotype.The serum samples from 24 HBV-induced liver failure patients and 23 healthy volunteers were collected and analyzed by gas chromatography-mass spectrometry(GC-MS)to generate metabolite profiles.The 24 patients were further grouped into two classes according to the severity of liver failure.Twenty-five commensal peaks in all metabolite profiles were extracted,and the relative area values of these peaks were used as features for each sample.Three algorithms,F-test,k-nearest neighbor(KNN)and fuzzy support vector machine(FSVM)combined with exhaustive search(ES),were employed to identify a subset of metabolites(biomarkers)that best predict liver failure.Based on the achieved experimental dataset,93.62%predictive accuracy by 6 features was selected with FSVM-ES and three key metabolites,glyceric acid,cis-aconitic acid and citric acid,are identified as potential diagnostic bio- markers. | Yong MAO Xin HUANG Ke YU Hai-bin QU Chang-xiao LIU Yi-yu CHENG | 2008 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2008，9，6： | 11 |
| 20 | Dopamine D4 Receptor Gene Associated with the Frontal-Striatal-Cerebellar Loop in Children with ADHD: A Resting-State fMRI Study显示文摘Attention deficit hyperactivity disorder(ADHD)is a common childhood neuropsychiatric disorder that has been linked to the dopaminergic system. This study aimed to investigate the effects of regulation of the dopamine D4 receptor(DRD4) on functional brain activity during the resting state in ADHD children using the methods of regional homogeneity(Re Ho) and functional connectivity(FC). Resting-state functional magnetic resonance imaging data were analyzed in 49 children with ADHD. All participants were classified as either carriers of the DRD44-repeat/4-repeat(4 R/4 R) allele(n = 30) or the DRD42-repeat(2 R) allele(n = 19). The results showed that participants with the DRD4 2 R allele had decreased Re Ho bilaterally in the posterior lobes of the cerebellum, while Re Ho was increased in the left angular gyrus. Compared with participants carrying the DRD4 4 R/4 R allele, those with the DRD4 2 R allele showed decreased FC to the left angular gyrus in the left striatum, right inferior frontal gyrus, and bilateral lobes of the cerebellum. The increased FC regions included the left superior frontal gyrus, medial frontal gyrus, and rectus gyrus. These data suggest that the DRD4 polymorphisms are associated with localized brain activity and specific functional connections, including abnormality in the frontal-striatal-cerebellar loop. Our study not only enhances the understanding of the correlation between the cerebellar lobes and ADHD, but also provides an imaging basis for explaining the neural mechanisms underlying ADHD in children. | ANDan Qian Xin Wang Huiru Liu Jiejie Tao Jiejie Zhou Qiong Ye Jiance Li Chuang Yang Jingliang Cheng Ke Zhao Meihao Wang | 2018 | Neuroscience Bulletin2018，34，3： | 10 |
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