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| 1 | Incidence and mortality of cervical cancer in China,2013显示文摘Objective: Estimating the incidence and mortality rate of cervical cancer became necessary to establish prevention measures and healthy policies.The aim of this study was to estimate the updated incidence and mortality rate of cervical cancer in 2013 in China.Methods: According to the evaluation criteria developed by the National Central Cancer Registry of China,the data submitted from 255 cancer registries met the required standards in 2013.Cervical cancer cases were retrieved from the national database and combined with the 2013 national population data.The age-standardized incidence and mortality rates were based on the demographic structure of the national census 2000 and Segi's world population.Results: In 2013,the estimated number of new cases and deaths from cervical cancer were 100,700 and 26,400,respectively.The crude incidence of cervical cancer was 15.17/100,000.The age-standardized incidence rates based on the Chinese standard population(ASIRC) and the world standard population(ASIRW) were 11.30/100,000 and10.30/100,000,respectively.The incidence of cervical cancer in urban areas was 15.62/100,000 and the ASIRC was11.12/100,000.The incidence of cervical cancer in rural areas was 14.65/100,000 and the ASIRC was11.47/100,000.The mortality rate of cervical cancer was 3.98/100,000.The age-standardized mortality rates based on the Chinese(ASMRC) and world standard populations(ASMRW) were 2.76/100,000 and 2.62/100,000,respectively.The mortality rate of cervical cancer in urban areas was 3.85/100,000 and in rural areas was4.14/100,000.Cervical cancer incidence and mortality increased with age.Urban areas had a higher incidence of cervical cancer and lower mortality rates when compared with rural areas.Conclusions: Dynamic monitoring of cervical cancer incidence and mortality is the fundamental work of cervical cancer prevention and control.Cervical cancer is a serious issue in women's health,and prevention strategies need to be enhanced,such as human papilloma virus(HPV) vaccination and screening programs. | Bingbing Song Chao Ding Wangyang Chen Huixin Sun Maoxiang Zhang Wanqing Chen | 2017 | Chinese Journal of Cancer Research2017，29，6： | 35 |
| 2 | Annual cost of illness of stomach and esophageal cancer patientsin urban and rural areas in China: A multi-center study显示文摘Objective: Stomach and esophageal cancer are imposing huge threats to the health of Chinese people whereas there were few studies on the financial burden of the two cancers.Methods: Costs per hospitalization of all patients with stomach or esophageal cancer discharged between September 2015 and August 2016 in seven cities/counties in China were collected, together with their demographic information and clinical details. Former patients in the same hospitals were sampled to collect information on annual direct non-medical cost, indirect costs and annual number of hospitalization. Annual direct medical cost was obtained by multiplying cost per hospitalization by annual number of hospitalization. Annual cost of illness(ACI)was obtained by adding the average value of annual direct medical cost, direct non-medical cost and indirect cost,stratified by sex, age, clinical stage, therapy and pathologic type in urban and rural areas. Costs per hospitalization were itemized into eight parts to calculate the proportion of each part. All costs were converted to 2016 US dollars(1 USD=6.6423 RMB).Results: Totally 19,986 cases were included, predominately male. Mean ages of stomach cancer and urban patients were lower than that of esophageal cancer and rural patients. ACI of stomach and esophageal cancer patients were $10,449 and $13,029 in urban areas, and $2,927 and $3,504 in rural areas, respectively. Greater ACI was associated with male, non-elderly patients as well as those who were in stage I and underwent surgeries.Western medicine fee took the largest proportion of cost per hospitalization.Conclusions: The ACI of stomach and esophageal cancer was tremendous and varied substantially among the population in China. Preferential policies of medical insurance should be designed to tackle with this burden and further reduce the health care inequalities. | Zhixun Yang Hongmei Zeng Ruyi Xia Qian Liu Kexin Sun Rongshou Zheng Siwei Zhang Changfa Xia He Li Shuzheng Liu Zhiyi Zhang Yuqin Liu Guizhou Guo Guohui Song Yigong Zhu Xianghong Wu Bingbing Song Xianzhen Liao Yanfang Chen Wenqiang Wei Guihua Zhuang Wanqing Chen | 2018 | Chinese Journal of Cancer Research2018，30，4： | 22 |
| 3 | Roles of a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism in ABA signal production in Arabidopsis显示文摘ABA, acting as a stress signal, plays crucial roles in plant resistance to water stress. Because ABA signal production is based on ABA biosynthesis, the regulation of NCED, a key enzyme in the ABA biosynthesis pathway, is normally thought of as the sole factor controlling ABA signal production. Here we demonstrate that ABA catabolism in combination with a synergistic regulation of ABA biosynthesis plays a crucial role in governing ABA signal production. Water stress induced a significant accumulation of ABA, which exhibited different patterns in detached and attached leaves. ABA catabolism followed a temporal trend of exponential decay for both basic and stress ABA, and there was little difference in the catabolic half-lives of basic ABA and stress ABA. Thus, the absolute rate of ABA catabolism, i.e. the amount of ABA catabolized per unit time, increases with increased ABA accumulation. From the dynamic processes of ABA biosynthesis and catabolism, it can be inferred that stress ABA accumulation may be governed by a synergistic regulation of all the steps in the ABA biosynthesis pathway. Moreover, to maintain an elevated level of stress ABA sustained activation of NCED3 should be required. This inference was supported by further findings that the genes encoding major enzymes in the ABA biosynthesis pathway, e.g. NCED3, AAO3 and ABA3 were all activated by water stress, and with ABA accumulation progressing, the expressions of NCED3, AAO3 and ABA3 remained activated. Data on ABA catabolism and gene expression jointly indicate that ABA signal production is controlled by a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism. | REN HuiBo FAN YiJian GAO ZhiHui WEI KaiFa LI GuiFen LIU Jing CHEN Lin LI BingBing HU JianFang JIA WenSuo | 2007 | Chinese Science Bulletin2007，52，4： | 12 |
| 4 | Integrated energy-oriented cruising control of electric vehicle on highway with varying slopes considering battery aging显示文摘Eco-driving strategies for vehicles with conventional powertrains have been studied for years and attempt to reduce fuel consumption by optimizing the driving velocity profile.For electric vehicles(EVs)with regenerative braking,the speed profile with the best energy efficiency should be different from conventional vehicles.This paper proposes an energy-oriented cruising control strategy for EVs with a hierarchical structure to realize eco-cruising on highways with varying slopes.The upper layer plans the energy-optimized vehicle velocity,and the lower layer calculates the torque allocation between the front and rear axles.However,the resulting speed profile with varying velocity may cause a high charge and discharge rate of the battery,resulting in rapid battery fading.To extend the battery life,we make a tradeoff between the energy consumption and wear of the battery by formulating an optimal control problem,where driving comfort and travel time are also considered.An indirect optimal control method is implemented to derive the optimal control rule.As an extension,the control rule for avoiding rear-end collisions is presented and simulated for driving in the real world. | ZHUANG WeiChao QU LingHu XU ShaoBing LI BingBing CHEN Nan YIN GuoDong | 2020 | Science China(Technological Sciences)2020，63，1： | 10 |
| 5 | Mechanical properties of electron beam welded dissimilar joints of TC17 and Ti60 alloys显示文摘Microstructure, hardness, tensile and high cycle fatigue(HCF) properties of the welded dissimilar joints of Ti60 and TC17 titanium alloys had been investigated in this study. A significant microstructural change was observed to occur after welding, with rod-like α and β phases in the fusion zone(FZ), equiaxed αphases, fine α laths and β phases in the heat-affected zone(HAZ) of TC17 side and acicular martensiteα' phases+ 'ghost' α phases in the HAZ of Ti60 side. The microhardness across the joints exhibited an inhomogeneous distribution with the highest hardness of ~404 HV in FZ and the lowest hardness of^304 HV in base material(BM) of Ti60. All the joints tested in tension fractured at BM of Ti60 side.Fatigue limits of the joints at 107 cycles were 425 MPa at room temperature and 380 MPa at 400℃,respectively. Welding micropores were found to be the main source of fatigue crack initiation. | Chao Cheng Bingbing Yu Zhiyong Chen Jianrong Liu Qingjiang Wang | 2018 | Journal of Materials Science & Technology2018，34，10： | 10 |
| 6 | Medical expenditures for colorectal cancer diagnosis and treatment: A 10-year high-level-hospital-based multicenter retrospective survey in China, 2002-2011显示文摘Objective: Colorectal cancer(CRC) causes a substantial burden of disease in China and the evidence of economic burden triggered is fundamental for priority setting. The aim of this survey was to quantify medical expenditures and the time trends for CRC diagnosis and treatment in China.Methods: From 2012 to 2014, a hospital-based multicenter retrospective survey was conducted in 13 provinces across China. For each eligible CRC patient diagnosed from 2002 to 2011, clinical information and expenditure data were extracted using a uniform questionnaire. All expenditure data were reported in Chinese Yuan(CNY)using 2011 values.Results: Of the 14,536 CRC patients included, the average age at diagnosis was 58.2 years and 15.8% were stageI cases. The average medical expenditure per patient was estimated at 37,902 CNY [95 % confidence interval(95%CI): 37,282-38,522], and the annual average increase rate was 9.2% from 2002 to 2011(P for trend <0.001), with a cumulative increase of 2.4 times(from 23,275 CNY to 56,010 CNY). The expenditure per patient in stages Ⅰ, Ⅱ, Ⅲ and Ⅳ were 31,698 CNY, 37,067 CNY, 38,918 CNY and 42,614 CNY, respectively(P<0.001). Expenditure significantly differed within various subgroups. Expenses for drugs contributed the largest proportion(52.6%).Conclusions: These conservative estimates illustrated that medical expenditures for CRC diagnosis and treatment in tertiary hospitals in China were substantial and increased rapidly over the 10 years, with drugs continually being the main expense by 2011. Relatively, medical expenditures are lower for CRC in the earlier stages. These findings will facilitate the economic evaluation of CRC prevention and control in China. | Jufang Shi Guoxiang Liu Hong Wang Ayan Mao Chengcheng Liu Lanwei Guo Huiyao Huang Jiansong Ren Xianzhen Liao Yana Bai Xiaojie Sun Xinyu Zhu Jialin Wang Bingbing Song Jinyi Zhou Lin Zhu Haike Lei Yuqin Liu Yunyong Liu Lingbin Du Yutong He Kai Zhang Ni Li Wanqing Chen Min Dai Jie He | 2019 | Chinese Journal of Cancer Research2019，31，5： | 8 |
| 7 | Microstructure Evolution and Tensile Properties of Ti_3Al/Ni-based Superalloy Welded Joint显示文摘In the present work,the dissimilar joining of a Ti_3AI-based alloy to a Ni-based superalloy was attempted by gas tungsten arc(GTA) welding technology.Sound joints were successfully achieved by using a Cu—Ni alloy as filler material.According to X-ray energy dispersive spectroscopy and X-ray diffraction analysis results three transitional layers at the weld/Ti_3AI interface were verified as follows:Ti_2AINb phase dissolved with Cu and Ni;AI(Cu,Ni)_2Ti,(Cu,Ni)_2Ti and(Nb,Ti) solid solution;Cu-rich phase and a complex multi-element phase.The In718/weld interface is characterized by solid solutions of Ni,Cu,Cr,Fe and Nb.The average tensile strength of the as-welded joints at room temperature is 1 63 MPa,and after a post—weld heat treatment it is increased slightly to 177 MPa.The fracture occurred at the surfacial layer of the joined Ti_3AI base alloy,indicating that the Ti_2AINb layer dissolved with Cu and Ni is the weak link of the Ti_3AI/ln71 8 joint. | Bingqing Chen Huaping Xiong Bingbing Sun Siyi Tang Shaoqing Guo Xuejun Zhang | 2014 | Journal of Materials Science & Technology2014，30，7： | 7 |
| 8 | Genome Editing as A Versatile Tool to Improve Horticultural Crop Qualities显示文摘The quality traits of horticultural crops,including the accumulation of nutrients and flavor substances,morphology,and texture,affect the palatability and nutritional value.For many years,efforts have been made to improve the quality of horticultural crops.The recent establishment of gene editing technology,with its potential applications in horticultural crops,provides a strategy for achieving this goal in a rapid and efficient manner.Here,we summarize research efforts aimed at improving horticultural crop quality through genome editing.We describe specific genome editing systems that have been used and traits that have been targeted in these efforts.Additionally,we discuss limiting factors and future perspectives of genome editing technology in improving horticultural crop qualities in both research and plant breeding.In summary,genome editing technology is emerging as a powerful tool for efficiently and rapidly improving horticultural crop quality,and we believe that the cautious application of genome editing in horticultural crops will generate new germplasms with improved quality in the near future. | Yating Chen Wenwen Mao Ting Liu Qianqian Feng Li Li Bingbing Li | 2020 | Horticultural Plant Journal2020，6，6： | 5 |
| 9 | Multifunctional SiC@SiO_(2) Nanofiber Aerogel with Ultrabroadband Electromagnetic Wave Absorption显示文摘Traditional ceramic materials are generally brittle and not flexible with high production costs,which seriously hinders their practical applications.Multifunctional nanofiber ceramic aerogels are highly desirable for applications in extreme environments,however,the integration of multiple functions in their preparation is extremely challenging.To tackle these challenges,we fabricated a multifunctional SiC@SiO_(2) nanofiber aerogel(SiC@SiO_(2) NFA)with a threedimensional(3D)porous cross-linked structure through a simple chemical vapor deposition method and subsequent heat-treatment process.The as-prepared SiC@SiO_(2) NFA exhibits an ultralow density(~11 mg cm^(-3)),ultra-elastic,fatigue-resistant and refractory performance,high temperature thermal stability,thermal insulation properties,and significant strain-dependent piezoresistive sensing behavior.Furthermore,the SiC@SiO_(2) NFA shows a superior electromagnetic wave absorption performance with a minimum refection loss(RL_(min))value of-50.36 d B and a maximum effective absorption bandwidth(EAB_(max))of 8.6 GHz.The successful preparation of this multifunctional aerogel material provides a promising prospect for the design and fabrication of the cutting-edge ceramic materials. | Limeng Song Fan Zhang Yongqiang Chen Li Guan Yanqiu Zhu Mao Chen Hailong Wang Budi Riza Putra Rui Zhang Bingbing Fan | 2022 | Nano-Micro Letters2022，14，9： | 4 |
| 10 | A highly selective dual-channel Hg^(2+) chemosensor based on an easy to prepare double naphthalene Schiff base显示文摘A non-sulfur chemosensor based on an easy to prepare double naphthalene Schiff base is reported for the colorimetric and fluorometric dual-channel sensing of Hg2+ ions by taking advantage of the twisted intramolecular charge transfer (TICT) mechanism. This work provides a novel approach for the selective recognition of mercury ions. Moreover, this sensor serves as a potential recyclable component in sensing materials and the complex L-Hg2+ (L = 1-[(2-naphthalenylimino)methyl]-2-naphthalenol) can therefore be used as a fluorescent sensor for iodine anions. Notably, the color changes are very significant and all the recognition and recycling processes can be observed by the naked eye. | ZHANG YouMing SHI BingBing ZHANG Peng HUO JianQiang CHEN Pei LIN Qi LIU Jun WEI TaiBao | 2013 | Science China Chemistry2013，56，5： | 4 |
| 11 | CRISPR/Cas9-introduced single and multiple mutagenesis in strawberry显示文摘CRISPR/Cas9-mediated genome editing is a powerful tool for life science research.Recently,strawberry (Fragaria × ananassa),an important horticultural crop,has emerged as a model organism for investigating the regulatory mechanisms of fruit development and ripening (Shulaev et al.,2011;Jia et al.,2013,2017;Kang et al.,2013;Han et al.,2015).While most cultivated strawberries (e.g.,F.× ananassa)are octoploid,most wild strawberries (e.g.,F.vesca)are diploid.Although CRISPR/Cas9 methods have been established in many plants,the use of this tool in strawberry needs to be further explored (Zhou et al.,2018). | Sinian Xing Meiru Jia Lingzhi Wei Wenwen Mao Usman Ali Abbasi Yaoyao Zhao Yating Chen Minglin Cao Kai Zhang Zhengrong Dai Zhechao Dou Wensuo Jia Bingbing Li | 2018 | Journal of Genetics and Genomics2018，45，12： | 4 |
| 12 | Hybrid speciation via inheritance of alternate alleles of parental isolating genes显示文摘It is increasingly realized that homoploid hybrid speciation(HHS),which involves no change in chromosome number,is an important mechanism of speciation.HHS will likely increase in frequency as ecological and geographical barriers between species are continuing to be disrupted by human activities.HHS requires the establishment of reproductive isolation between a hybrid and its parents,but the underlying genes and genetic mechanisms remain largely unknown.In this study,we reveal by integrated approaches that reproductive isolation originates in one homoploid hybrid plant species through the inheritance of altemate alleles at genes that determine parental premating isolation.The parent species of this hybrid species are reproductively isolated by differences in flowering time and survivorship on soils containing high concentrations of iron.We found that the hybrid species inherits alleles of parental isolating major genes related to flowering time from one parent and alleles of major genes related to iron tolerance from the other parent.In this way,it became reproductively isolated from one parent by the difference in flowering time and from the other by habitat adaptation(iron tolerance).These findings and further modeling results suggest that HHS may occur relatively easily via the inheritance of alternate parental premating isolating genes and barriers. | Zefu Wang Yuanzhong Jiang Hao Bi Zhiqiang Lu Yazhen Ma Xiaoyue Yang Ningning Chen Bin Tian Bingbing Liu Xingxing Mao Tao Ma Stephen P.DiFazio Quanjun Hu Richard J.Abbott Jianquan Liu | 2021 | Molecular Plant2021，14，2： | 4 |
| 13 | An insight into failure mechanism of NASICON-structured Na3V2(PO4)3 in hybrid aqueous rechargeable battery显示文摘NASICON (Na-super-ionic-conductors)-structured materials have attracted extensive research interest due to their great application potential in secondary batteries. However, the mechanism of capacity fading for NASICON-structured electrode materials has been rarely studied. In this paper, we synthesized the NASICON-structured Na3V2(PO4)3/C composite by simple sol-gel and high-temperature solid-phase method and investigated its electrochemical performance in Na-Zn hybrid aqueous rechargeable batteries. After characterizing the structure, morphology and composition variations as well as the interfacial resistance changes of Na3V2(PO4)3/C cathode during cycling, we propose a mechanical and interfacial degradation mechanism for capacity fading of NASICON-structured Na3V2(PO4)3/C in Na-Zn hybrid aqueous rechargeable batteries. This work will shed light on enhancing the mechanical and in terfacial stability of NASICON-structured Na3V2(PO4)3/C in Na-Zn hybrid aqueous rechargeable batteries. | Xinxin Zhang Jun Ma Pu Hu Bingbing Chen Chenglong Lu Xinhong Zhou Pengxian Han Lihua Chen Guanglei Cui | 2019 | Journal of Energy Chemistry2019，28，5： | 3 |
| 14 | CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus显示文摘 | Qi Liu Yu Jiang Lei Shao Ping Yang Bingbing Sun Sheng Yang Daijie Chen | 2017 | Acta Biochimica et Biophysica Sinica2017，49，9： | 3 |
| 15 | Databases and Web Tools for Cancer Genomics Study显示文摘Publicly-accessible resources have promoted the advance of scientific discovery. The era of genomics and big data has brought the need for collaboration and data sharing in order to make effective use of this new knowledge. Here, we describe the web resources for cancer genomics research and rate them on the basis of the diversity of cancer types, sample size, omics data comprehensiveness, and user experience. The resources reviewed include data repository and analysis tools; and we hope such introduction will promote the awareness and facilitate the usage of these resources in the cancer research community. | Yadong Yang Xunong Dong Bingbing Xie Nan Ding Juan Chen Yongjun Li Qian Zhang Hongzhu Qu Xiangdong Fang | 2015 | Genomics, Proteomics & Bioinformatics2015，13，1： | 3 |
| 16 | Microstructures and mechanical properties of Ti_3Al/Ni-based superalloy joints arc welded with Ti–Nb and Ti–Ni–Nb filler alloys显示文摘Dissimilar joining of Ti3Al-based alloy to Ni-based superalloy has been carried out using gas tungsten arc(GTA) welding technology with Ti–Nb and Ti–Ni–Nb filler alloys.The joint welded with the Ti–Nb filler alloy contained much less interfacial brittle phases than the one using the Ti–Ni–Nb filler alloy.The average room-temperature tensile strength of the joint welded with Ti–Nb was 202 MPa and the strength value of the one welded with Ti–Ni–Nb was 128 MPa.For both fillers,the weak links of the dissimilar joints were the weld/In718 interfaces.The presence of TiNi,TiNi3 and Ni3Nb intermetallic compounds in the joint welded with Ti–Ni–Nb induced microcracks at the weld/In718 interface and deteriorated the mechanical properties of the joint.And the adoption of the Ti–Nb filler alloy decreased the formation tendency of interfacial brittle phases to some extent and thus enhanced the tensile strength of the joint. | Bingqing Chen Huaping Xiong Bingbing Sun Siyi Tang Borui Du Neng Li | 2014 | Progress in Natural Science:Materials International2014，24，4： | 3 |
| 17 | Solitary beam propagation in periodic layered Kerr media enables high-efficiency pulse compression and mode self-cleaning显示文摘Generating intense ultrashort pulses with high-quality spatial modes is crucial for ultrafast and strong-field science and can be achieved by nonlinear supercontinuum generation(SCG)and pulse compression.In this work,we propose that the generation of quasi-stationary solitons in periodic layered Kerr media can greatly enhance the nonlinear lightmatter interaction and fundamentally improve the performance of SCG and pulse compression in condensed media.With both experimental and theoretical studies,we successfully identify these solitary modes and reveal their unified condition for stability.Space-time coupling is shown to strongly influence the stability of solitons,leading to variations in the spectral,spatial and temporal profiles of femtosecond pulses.Taking advantage of the unique characteristics of these solitary modes,we first demonstrate single-stage SCG and the compression of femtosecond pulses from 170 to 22 fs with an efficiency>85%.The high spatiotemporal quality of the compressed pulses is further confirmed by highharmonic generation.We also provide evidence of efficient mode self-cleaning,which suggests rich spatiotemporal self-organization of the laser beams in a nonlinear resonator This work offers a route towards highly efficient,simple,stable and highly flexible SCG and pulse compression solutions for state-of-the-art ytterbium laser technology. | Sheng Zhang Zongyuan Fu Bingbing Zhu Guangyu Fan Yudong Chen Shunjia Wang Yaxin Liu Andrius Baltuska Cheng Jin Chuanshan Tian Zhensheng Tao | 2021 | Light(Science & Applications)2021，10，4： | 3 |
| 18 | Highly active sites of low spin Fe^(Ⅱ)N_(4)species:The identification and the ORR performance显示文摘Over recent years,catalytic materials of Fe-N-C species have been recognized being active for oxygen reduction reaction(ORR).However,the identification of active site remains challenging as it generally involves a pyrolysis process and mixed components being obtained.Herein Fe_(3)C/C and Fe_(2)N/C samples were synthesized by temperature programmed reduction of Fe precursors in 15%CH_(4)/H_(2)and pure NH_(3),respectively.By acid leaching of Fe_(2)N/C sample,only single sites of FeN_(4)species were presented,providing an ideal model for identification of catalytic functions of the single sites of FeN_(4)in ORR.A correlation was conducted between the concentration of Fe^(Ⅱ)N_(4)in low spin state by Mossbauer spectra and the kinetic current density at 0.8 V in alkaline media,and such a structure-performance correlation assures the catalytic roles of low spin Fe^(Ⅱ)N_(4) species as highly active sites for the ORR. | Huizhu Cai Bingbing Chen Xiao Zhang Yuchen Deng Dequan Xiao Ding Ma Chuan Shi | 2021 | Nano Research2021，14，1： | 2 |
| 19 | High-risk phenotypes of genetic disease in a Neonatal Intensive Care Unit population显示文摘To the Editor: Genetic diseases contribute to 35% of deaths during the first year of life and are a significant cause of intensive care.[1] A previous study based on the China Neonatal Genomes Project investigated the genetic causes of early infant deaths and found that >25% of deceased neonates with genetic diagnoses can be cured if diagnosed in time.[2] Therefore, it is crucial to target and diagnose neonates with genetic diseases as early as possible. According to our experience, the typical phenotypes, such as special facial features or multiple congenital anomalies (MCAs), indicate a high risk of genetic disease and lead physicians to perform genetic testing in neonates as early as possible. However, in practice, infants without typical phenotypes typically undergo a long and costly diagnostic process before genetic diagnoses are confirmed. Moreover, a recent survey by the American College of Medical Genetics and Genomics (ACMG) and other national professional organizations indicated that there are insufficient numbers of qualified geneticists to fulfil genetic service needs.[3] The ACMG published the general clinical features for genetic testing indications. For example, patients with phenotypes or family history data that strongly implicate a genetic cause may undergo genetic testing.[1] However, the study indicated that many genetic conditions arise de novo or are inherited with no family history.[1] A previous study attempted to apply the non-phenotype-driven panel approach in neonates admitted to the neonate intensive care unit (NICU).[4] However, at present, the diagnostic yield is only 3.45% (1/29).[4] In addition, the economic and ethical issues associated with genomic screening remain challenging. Therefore, the available indications for genetic testing may improve the management of genetic diseases. | Tiantian Xiao Qi Ni Huiyao Chen Huijun Wang Lin Yang Bingbing Wu Yun Cao Guoqiang Cheng Laishuan Wang Liyuan Hu Hongfang Mei Yulan Lu Mengchun Gong Xinran Dong Wenhao Zhou | 2022 | Chinese Medical Journal2022，，5： | 2 |
| 20 | A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)-associated nuclease 9(Cas9)-based genome editing tool pCas/pTargetF system that we established previously has been widely used in Escherichia coli MG1655.However,this system failed to manipulate the genome of E.coli BL21(DE3),owing to the potential higher leaky transcription of the gRNA-pMB1 specific to pTargetF in this strain.In this study,we modified the pCas/pTargetF system by replacing the promoter of gRNA-pMB1 with a tightly regulated promoter PrhaB,changing the replicon of pCas to a nontemperature-sensitive replicon,adding the sacB gene into pCas,and replacing the original N20-specific sequence of pTargetF with ccdB gene.We call this updated system as pEcCas/pEcgRNA.We found that gRNA-pMB1 indeed showed a slightly higher leaky expression in the pCas/pTargetF system compared with pEcCas/pEcgRNA.We also confirmed that genome editing can successfully be performed in BL21(DE3)by pEcCas/pEcgRNA with high efficiency.The application of pEcCas/pEcgRNA was then expanded to the E.coli B strain BL21 Star^(TM)(DE3),K-12 strains MG1655,DH5α,CGMCC3705,Nissle1917,W strain ATCC9637,and also another species of Enterobacteriaceae,Tatumella citrea DSM13699,without any specific modifications.Finally,the plasmid curing process was optimized to shorten the time from|$\sim$|60 h to|$\sim$|32 h.The entire protocol(including plasmid construction,editing,electroporation and mutant verification,and plasmid elimination)took only|$\sim$|5.5 days per round in the pEcCas/pEcgRNA system,whereas it took|$\sim$|7.5 days in the pCas/pTargetF system.This study established a faster-acting genome editing tool that can be used in a wider range of E.coli strains and will also be useful for other Enterobacteriaceae species. | Qi Li Bingbing Sun Jun Chen Yiwen Zhang Yu Jiang Sheng Yang | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 2 |
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