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| 1 | A complex structure of arrestin-2 bound to a G protein-coupled receptor显示文摘Arrestins comprise a family of signal regulators of G-protein-coupled receptors(GPCRs),which include arrestins 1 to 4.While arrestins 1 and 4 are visual arrestins dedicated to rhodopsin,arrestins 2 and 3(Arr2 and Arr3)areβ-arrestins known to regulate many nonvisual GPCRs.The dynamic and promiscuous coupling of Arr2 to nonvisual GPCRs has posed technical challenges to tackle the basis of arrestin binding to GPCRs.Here we report the structure of Arr2 in complex with neurotensin receptor 1(NTSR1),which reveals an overall assembly that is strikingly different from the visual arrestin–rhodopsin complex by a 90°rotation of Arr2 relative to the receptor.In this new configuration,intracellular loop 3(ICL3)and transmembrane helix 6(TM6)of the receptor are oriented toward the N-terminal domain of the arrestin,making it possible for GPCRs that lack the C-terminal tail to couple Arr2 through their ICL3.Molecular dynamics simulation and crosslinking data further support the assembly of the Arr2‒NTSR1 complex.Sequence analysis and homology modeling suggest that the Arr2‒NTSR1 complex structure may provide an alternative template for modeling arrestin–GPCR interactions. | Wanchao Yin Zhihai Li Mingliang Jin Yu-Ling Yin Parker Wde Waal Kuntal Pal Yanting Yin Xiang Gao Yuanzheng He Jing Gao Xiaoxi Wang Yan Zhang Hu Zhou Karsten Melcher Yi Jiang Yao Cong X.Edward Zhou Xuekui Yu H.Eric Xu | 2019 | Cell Research2019，29，12： | 4 |
| 2 | Platelet-mediated adhesion facilitates leukocyte sequestration in hypoxia-reoxygenated microvessels显示文摘Leukocyte transendothelial migration and sequestration are two distinct outcomes following leukocyte adhesion to endothelium during ischemia-reperfusion injury, in which platelets may play a pivotal role. In the present study, we established an in vitro hypoxia-reoxygenation model to mimic ischemia-reperfusion injury and found platelet pre-incubation significantly increased leukocyte adhesion to endothelial cells after hyoxia-reoxygenation(over 67%). Blockade of endothelial-cell-expressed adhesion molecules inhibited leukocyte direct adhesion to endothelial cells, while platelet-mediated leukocyte adhesion was suppressed by blockade of platelet-expressed adhesion molecules. Further experiments revealed platelets acted as a bridge to mediate leukocyte adhesion, and platelet-mediated adhesion was the predominant pattern in the presence of platelets. However, platelet pre-incubation significantly suppressed leukocyte transendothelial migration after hypoxia-reoxygenation(over 31%), which could be aggravated by blockade of endothelial-cell-expressed adhesion molecules, but alleviated by blockade of platelet-expressed adhesion molecules. This would indicate that platelet-mediated adhesion disrupted leukocyte transendothelial migration. An in vivo mesenteric ischemia-reperfusion model demonstrated leukocyte transfusion alone caused mild leukocyte adhesion to reperfused vessels and subsequent leukocyte infiltration, while simultaneous leukocyte and platelet transfusion led to massive leukocyte adhesion and sequestration within reperfused microvessels. Our studies revealed platelets enhanced leukocyte adhesion to endothelial cells, but suppressed leukocyte transendothelial migration. Overall, this leads to leukocyte sequestration in hypoxia-reoxygenated microvessels. | Senfeng Zheng Yanting Cao Wenjian Zhang Honglin Liu Jia You Yiqing Yin Jinning Lou Chenghui Li | 2016 | Science China(Life Sciences)2016，59，3： | 3 |
| 3 | PINK1 kinase dysfunction triggers neurodegeneration in the primate brain without impacting mitochondrial homeostasis显示文摘In vitro studies have established the prevalent theory that the mitochondrial kinase PINK1 protects neurodegeneration by removing damaged mitochondria in Parkinson's disease(PD).However,difficulty in detecting endogenous PINK1 protein in rodent brains and cell lines has prevented the rigorous investigation of the in vivo role of PINK1.Here we report that PINK1 kinase form is selectively expressed in the human and monkey brains.CRISPR/Cas9-mediated deficiency of PINK1 causes similar neurodegeneration in the brains of fetal and adult monkeys as well as cultured monkey neurons without affecting mitochondrial protein expression and morphology.Importantly,PINK1 mutations in the primate brain and human cells reduce protein phosphorylation that is important for neuronal function and survival.Our findings suggest that PINK1 kinase activity rather than its mitochondrial function is essential for the neuronal survival in the primate brains and that its kinase dysfunction could be involved in the pathogenesis of PD. | Weili Yang Xiangyu Guo Zhuchi Tu Xiusheng Chen Rui Han Yanting Liu Sen Yan Qi Wang Zhifu Wang Xianxian Zhao Yunpeng Zhang Xin Xiong Huiming Yang Peng Yin Huida Wan Xingxing Chen Jifeng Guo Xiao-Xin Yan Lujian Liao Shihua Li Xiao-Jiang Li | 2022 | Protein & Cell2022，13，1： | 3 |
| 4 | Molecular assembly of rhodopsin with G protein-coupled receptor kinases显示文摘 | He, Yuanzheng Gao, Xiang Goswami, Devrishi Hou, Li Pal, Kuntal Yin, Yanting Zhao, Gongpu Ernst, Oliver P. Griffin, Patrick Melcher, Karsten Xu, H. Eric | 2017 | Cell Research2017，27，6： | 2 |
| 5 | Quantification,morphology and source of humic acid,kerogen and black carbon in offshore marine sediments from Xiamen Gulf,China显示文摘Three types of macromolecular organic matters (MOMs), i.e. humic acid (HA), kerogen+black carbon (KB), and black carbon (BC) were extracted from marine sediments of Xiamen Gulf, southeast of China. The chemical composition, morphological property and source of the three extractions were characterized by elemental analyzer/isotope ratio mass spectrometry (EA/IRMS) and scanning electron microscope (SEM). The results showed that KB was the predominant fraction in MOMs, which accounted for 61.79%-89.15% of the total organic content (TOC), while HA consisted less than 5%. The relative high contents of kerogen and BC, and low contents of HA in the samples indicated that anthropogenic input might be the major source of organic matter in marine sediments near the industrial regions. The characterization of SEM, not only revealed morphological properties of the three fractions, but also allowed a better understanding of the source of MOMs. The δ 13 C values of the three fractions suggested that materials from terrestrial C 3 plants were predominant. Furthermore, the anthropogenic activities, such as the discharge of sewage, coal and biomass combustion from industry nearby and agricultural practices within drainage basin of the Jiulong River, were remarkably contributed to the variations in δ 13 C values of MOMs in the offshore marine sediments. | Yanting Chen Jinping Zhao Liqian Yin Jinsheng Chen Dongxing Yuan | 2013 | Journal of Environmental Sciences2013，25，2： | 2 |
| 6 | A Probe into China's Near Term Finished Petroleum Products Market显示文摘自从 2008 ,这篇文章在中国在完成的石油产品的消费描述了变化,指颠倒了完成的油产品,和中国状况和油定价政策和主要因素与在工业 sectors.This 的变化联系了的国内经济的消费的国际经济环境文章,由在未来市场趋势上做设计,估计了在可预见的未来。 | Duan Yanting Yin Zhe (Beijing University of Technology,Beijing 100124) | 2009 | China Petroleum Processing & Petrochemical Technology2009，11，3： | 0 |
| 7 | Sensitivity of livelihood strategy to livestock production and marketization:An empirical analysis of grasslands in Inner Mongolia,China显示文摘Recent researches have primarily focused on the relationship between livelihood strategies and livelihood capital,with few empirical studies on the sensitivity of livelihood strategies to livestock production and marketization in Inner Mongolia Autonomous Region of China.This study used an income distribution approach to categorize livelihood strategies of the respondents(n=394)into three types,i.e.,herder livelihood strategy(LS1),petty-herder livelihood strategy(LS2),and non-herder livelihood strategy(LS3).Using the multinomial logistic regression model,we compared livestock production and marketization across the three livelihood strategies.Our findings showed that(1)livestock production and marketization tended to favor LS1;(2)an increase in the land asset(contracted and rented grassland)and off-take rate increased the probability of households choosing LS1;(3)stocking rate was higher for LS1;and(4)the higher critical market-related risks perceived by herders were animal price and hay and corn price.Moreover,higher livestock price acted as a deterrent to diversifying into other livelihood strategies(LS2 and LS3).Finally,this study advocates for policies that will promote the land transfer market,adopt modern techniques in animal husbandry,improve the medium for disseminating market information to herders,and provide incentives for long-term livelihood transformation. | Saheed Olaide JIMOH DING Wenqiang DONG Haibin BAI Haihua YIN Yanting LIU Huihui HOU Xiangyang | 2021 | Regional Sustainability2021，2，4： | 0 |
| 8 | Market opportunities do not explain the ability of herders to meet livelihood objectives over winter on the Mongolian Plateau显示文摘Drylands under pastoral land use are considered one of the most vulnerable social-ecological systems to global climate change,but the herders'abilities to adapt to the different extreme weather events have received little attention in the drylands.Herders on the Mongolian Plateau(MP;including Inner Mongolia Autonomous Region of China and Mongolia),have had a long history of adapting climatic variability and extreme weather events.However,it is unclear how changes such as increased levels of infrastructure and market integration affect the ability of herders to achieve the key livelihood objectives:the minimisation of the death and abortion rates of livestock in the winter.Here,we used remotely sensed and household survey data to map,model and explore the climate exposure and sensitivity of herders in the settled area(Inner Mongolia of China)and nomadic area(Mongolia)in the winter of 2012–2013.We aimed to quantify the multi-scaled characteristics of both climate exposure and sensitivity through the lens of key adaptive strategies utilized by herders.Our results showed that the higher levels of infrastructure and market integration,and the lower levels of remoteness on the MP did not increase the herders'ability to achieve the key livelihood objectives.Our results also suggested that exposure to the snow that is comparatively greater than the long-term average(cumulative exposure)may be more important in determining the social-ecological vulnerability than absolute exposure.We suggested that neither the risk management strategies available to these herders,nor the demographic variables,could compensate for the mode of production governing the pastoral systems.Our study could provide further evidence for the complex and scaled nature of climate exposure and sensitivity,and the results imply that any analysis of the relationship among exposure,sensitivity and vulnerability of pastoral households to climate change in the drylands will require a multi-scaled and interdisciplinary approach. | BAI Haihua YIN Yanting Jane ADDISON HOU Yulu WANG Linhe HOU Xiangyang | 2020 | Journal of Arid Land2020，12，3： | 0 |
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