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| 1 | A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2显示文摘The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus,and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.On the other hand,several concerning SARS-CoV-2 variants,including Omicron,are required to be identified as soon as the samples are identified as‘positive’.Unfortunately,a traditional PCR test does not allow their specific identification.Herein,for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing),which combines an optical sensing technology-surface plasmon resonance(SPR)with the‘gene scissors’clustered regularly interspaced short palindromic repeat(CRISPR)technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants.MOPCS is a low-cost,CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA,without the need for amplification,within 38 min from sample input to results output,and achieve a limit of detection of 15 fM.MOPCS achieves a highly sensitive analysis of SARS-CoV-2,and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron)and BA.1(a subtype of Omicron).This platform was also used to analyze some recently collected patient samples from a local outbreak in China,identified by the Centers for Disease Control and Prevention.This innovative CRISPR-empowered SPR platform will further contribute to the fast,sensitive and accurate detection of target nucleic acid sequences with single-base mutations. | Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar AAl-Hartomy Swelm Wageh Abdullah GAl-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang | 2022 | National Science Review2022，9，8： | 3 |
| 2 | AIEgens in cell-based multiplex fluorescence imaging显示文摘Fluorescence imaging is an important branch of bioimaging.It is non-invasive and provides superior spatial and temporal resolution during the real-time monitoring of biological samples of interest.Although the spatial resolution limit of optical microscopes is about 200 nm,due to the diffraction limit,with the application of super-resolution fluorescence microscopy technologies this limit has been pushed below 30 nm.This makes it feasible to visualize biological structures in subcellular levels and to monitor subcellular biological processes in real time.However,due to the complexity of the biological structure and components within cells,simultaneous staining and monitoring multiple intracellular components with different coloured fluorophores is often needed during multiplex imaging,to better understand biological processes.Aggregation-induced emission luminogens(AIEgen)and AIEgen based nanoparticles(NPs)have presented many advantages in fluorescence imaging,with strong potential for biological science and nano-medicine.Herein this review,we focus on the advantages of AIEgen and AIEgen NP in cell-based fluorescence imaging,and the latest advances of AIEgens in cell-based multiplex imaging are summarized and discussed.The future perspectives are proposed. | Yabin Zhou Jin Hua Ben Zhong Tang Youhong Tang | 2019 | Science China Chemistry2019，62，10： | 3 |
| 3 | CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant显示文摘Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing techniques.Herein,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 variant.To enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold nanoparticles.Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays.The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system.Furthermore,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing. | Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar AAl-Hartomy SWageh Abdullah GAl-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang | 2022 | Nano-Micro Letters2022，14，10： | 2 |
| 4 | Machining Deformation Prediction of Thin-Walled Part Based on Finite Element Analysis显示文摘For the problems of machining distortion and the low accepted product during milling process of aluminum alloy thin-walled part,this paper starts from the analysis of initial stress state in material preparation process,the change process of residual stress within aluminum alloy pre-stretching plate is researched,and the distribution law of residual stress is indirectly obtained by delamination measurement methods,so the effect of internal residual stress on machining distortion is considered before finite element simulation. Considering the coupling effects of residual stress,dynamic milling force and clamping force on machining distortion,a threedimensional dynamic finite element simulation model is established,and the whole cutting process is simulated from the blank material to finished product,a novel prediction method is proposed,which can availably predict the machining distortion accurately. The machining distortion state of the thin-walled part is achieved at different processing steps,the machining distortion of the thin-walled part is detected with three coordinate measuring machine tools,show that the simulation results are in good agreement with experimental data. | Hongxiang Wang Yabin Tang Zhanshan Liu Shi Gao | 2015 | Journal of Harbin Institute of Technology(New Series)2015，22，4： | 2 |
| 5 | Multiplexed imaging detection of live cell intracellular changes in early apoptosis with aggregation-induced emission fluorogens显示文摘Apoptosis is an important process for maintaining tissue homeostasis and eliminating abnormal cells in multicellular organisms.Abnormality in apoptosis often leads to severe diseases such as cancers. Better understanding of its mechanisms and processes is therefore important. Accompanying molecular biology events of apoptosis is a series of cellular morphology changes: nucleus condensation, cell shrinkage and rounding, cell surface blebbing, dynamic blebbing, apoptotic membrane protrusions and nucleus fragmentations and finally, the formation and release of apoptotic bodies. It is difficult to detect cellular changes in the early phase of apoptosis due to the subtle changes at this phase. In the current study, we induced apoptosis in He La cells with H2 O2 and used nuclear dye Hoechst 33258, mitochondria, lysosome and cytoplasmic protein specific aggregation-induced emission fluorogens(AIEgens), TPE-Ph-In, 2 M-DABS and BSPOTPE to successfully perform live cell multiplexed imaging to investigate early apoptosis cellular events. We showed the gradual dissipation of mitochondria membrane potential until it is nondetectable by TPE-Ph-In. Increased mitophagy detected by TPE-Ph-In and 2 M-DABS, condensed nucleus detected by Hoechst33258, increased permeability and/or reduced integrity of nuclear membrane, and increased intracellular vesicles detected by2 M-DABS are some of the early events of apoptosis. | Yabin Zhou Haixiang Liu Na Zhao Zhiming Wang Michael Z.Michael Ni Xie Ben Zhong Tang Youhong Tang | 2018 | Science China Chemistry2018，61，8： | 2 |
| 6 | Genetic polymorphisms in alveolar macrophage response-related genes, and risk of silicosis and pulmonary tuberculosis in Chinese iron miners显示文摘 | Yabin Qu Yunxia Tang Duozhi Cao Fen Wu Jing Liu Guoliang Lu Zhongbin Zhang Zhaolin Xia | 2006 | International Journal of Hygiene and Environmental Health2006，，6： | 1 |
| 7 | Connection between earthquakes and deep fluids revealed by magnetotelluric imaging in Songyuan,China显示文摘Songyuan is the most earthquake prone area in northeast China.Since 2006,earthquakes have occurred in the area in the form of swarms,with a maximum magnitude of M_L5.8.There is much controversy about the cause of the Songyuan earthquakes.We attempted to determine the cause using a three-dimensional electrical conductivity structure inverted from a regional network of magnetotelluric data in the Songyuan area.The L-BFGS inversion method was applied,with a fullimpedance tensor data set used as the inversion input.Combined with an evaluation of the earthquake locations,the resistivity model revealed a northeast-oriented hidden fault running through the Songyuan earthquake area(SEA),which was speculated to be the preexisting Fuyu-Zhaodong Fault(FZF).Our resistivity model also found an apparent lithospheric low-resistivity anomaly beneath the earthquake area,which breached the high-resistivity lithospheric mantle and stalled at the base of the crust.A petrophysical analysis showed that this lower crustal low-resistivity anomaly was most likely attributed to hydrated partial melting,which could release water into the lower crust during later magma emplacements.While weakening the strength of the FZF,these ascending fluids also increased the pore pressure in the fault,further reducing the shear strength of the fault.Shear stress action(a fault strike component of the east-west regional compress),together with possible near-surface disturbances,may drive the fault to slip and trigger the earthquakes in Songyuan.It is possible that the continuous replenishment of fluids from the deeper mantle forces the Songyuan earthquakes into the form of swarms.We infer that the Songyuan earthquakes could be attributed to a combination of preexisting faults,regional stress,and deep fluids associated with plate subduction,and near surface disturbances might induce the earthquakes in advance.The Songyuan earthquakes are inherently induced earthquakes,fed by deep fluids. | Yu TANG Aihua WENG Yue YANG Shiwen LI Jianjun NIU Yanhui ZHANG Yabin LI Jianping LI | 2021 | Science China Earth Sciences2021，64，1： | 1 |
| 8 | Genetic polymor- phisms in alveolar macrophage response-related genes, and risk of silicosis and pulmonary tuberculosis in Chinese Iron miners 显示文摘 | Qu Yabin Tang Yunxia Cao Duozhi | 2007 | Int J Hyg Environ Health2007，210，6： | 1 |
| 9 | Role of wide contacts in the at response of a double-barrier stractare显示文摘 | Quan Jun Yu Yabin Tang Y H | 2006 | Phys Rev B2006，73，19： | 1 |
| 10 | Impact of coke dry quenching process on coke quality显示文摘 | LU JIN HE YABIN TANG CHANGGENG | 2002 | Iron and Steel2002，37，1： | 1 |
| 11 | The role of the metastasis suppressor gene KAI 1 in melanoma angiogenesis显示文摘 | Yun Tang Madhuri Bhandaru Yabin Cheng Jing Lu Gang Li Christopher J. Ong | 2015 | Pigment Cell Melanoma Res2015，，6： | 1 |
| 12 | Aggregation-induced Emission Fluorogen as Mammalian Cell Cytoplasmic Tracker with Long Retention Time and High Photo-stability显示文摘Fluorescent probes used for cell imaging are powerful tools in cell-based assays and research.In this study,we exhibited a water-soluble aggregation-induced emission fluorogen(AIEgen),BSPO-TPE,specifically stained cytoplasm in live cells and had an excellent photostability when compared to that of two widely used commercial fluorescent dyes.The long cytoplasm retention time of BSPO-TPE demonstrated its suitability as a live cell cytoplasm tracker. | ZHOU Yabin HUA Jin ZHANG Hong-ping TANG Youhong | 2021 | Chemical Research in Chinese Universities2021，37，1： | 0 |
| 13 | Culture of Eriocheir sinensis Larvae in Ponds in Greenhouse显示文摘A total of 15 pools were selected from a greenhouse, and they were randomly and evenly divided into three groups.In each group, one stocking density of Eriocheir sinensis was arranged. The results showed that after 45-d culture, the crab number per kilogram reached about 300 with survival rate of about 20%; the initial stocking density had significantly effect on the body size of E. sinensis on sell, instead on the survival rate of E. sinensis larvae. Therefore, it is feasible to conduct the culture of E. sinensis larvae in a pool in greenhouse. | Guoliang RUAN Liangcheng HU Hai ZHOU Dawen MA Yabin TANG | 2016 | Agricultural Science & Technology2016，17，3： | 0 |
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