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| 1 | Enhanced production of seed oil with improved fatty acid composition by overexpressing NAD^(+)-dependent glycerol-3-phosphate dehydrogenase in soybean显示文摘There is growing interest in expanding the production of soybean oils(mainly triacylglycerol, or TAG) to meet rising feed demand and address global energy concerns. We report that a plastidlocalized glycerol-3-phosphate dehydrogenase(GPDH), encoded by GmGPDHp1 gene, catalyzes the formation of glycerol-3-phosphate(G3 P), an obligate substrate required for TAG biosynthesis.Overexpression of GmGPDHp1 increases soybean seed oil content with high levels of unsaturated fatty acids(FAs), especially oleic acid(C18:1), without detectably affecting growth or seed protein content or seed weight. Based on the lipidomic analyses, we found that the increase in G3 P content led to an elevated diacylglycerol(DAG) pool, in which the Kennedy pathwayderived DAG was mostly increased, followed by PC-derived DAG, thereby promoting the synthesis of TAG containing relatively high proportion of C18:1. The increased G3 P levels induced several transcriptional alterations of genes involved in the glycerolipid pathways. In particular, genes encoding the enzymes responsible for de novo glycerolipid synthesis were largely upregulated in the transgenic lines, in-line with the identified biochemical phenotype. These results reveal a key role for GmGPDHp1-mediated G3 P metabolism in enhancing TAG synthesis and demonstrate a strategy to modify the FA compositions of soybean oils for improved nutrition and biofuel. | Ying Zhao Pan Cao Yifan Cui Dongxu Liu Jiapeng Li Yabin Zhao Siqi Yang Bo Zhang Runnan Zhou Minghao Sun Xuetian Guo Mingliang Yang Dawei Xin Zhanguo Zhang Xin Li Chen Lv Chunyan Liu Zhaoming Qi Jingyu Xu Xiaoxia Wu Qingshan Chen | 2021 | Journal of Integrative Plant Biology2021，63，6： | 4 |
| 2 | AIEgens in cell-based multiplex fluorescence imaging显示文摘Fluorescence imaging is an important branch of bioimaging.It is non-invasive and provides superior spatial and temporal resolution during the real-time monitoring of biological samples of interest.Although the spatial resolution limit of optical microscopes is about 200 nm,due to the diffraction limit,with the application of super-resolution fluorescence microscopy technologies this limit has been pushed below 30 nm.This makes it feasible to visualize biological structures in subcellular levels and to monitor subcellular biological processes in real time.However,due to the complexity of the biological structure and components within cells,simultaneous staining and monitoring multiple intracellular components with different coloured fluorophores is often needed during multiplex imaging,to better understand biological processes.Aggregation-induced emission luminogens(AIEgen)and AIEgen based nanoparticles(NPs)have presented many advantages in fluorescence imaging,with strong potential for biological science and nano-medicine.Herein this review,we focus on the advantages of AIEgen and AIEgen NP in cell-based fluorescence imaging,and the latest advances of AIEgens in cell-based multiplex imaging are summarized and discussed.The future perspectives are proposed. | Yabin Zhou Jin Hua Ben Zhong Tang Youhong Tang | 2019 | Science China Chemistry2019，62，10： | 3 |
| 3 | Multiplexed imaging detection of live cell intracellular changes in early apoptosis with aggregation-induced emission fluorogens显示文摘Apoptosis is an important process for maintaining tissue homeostasis and eliminating abnormal cells in multicellular organisms.Abnormality in apoptosis often leads to severe diseases such as cancers. Better understanding of its mechanisms and processes is therefore important. Accompanying molecular biology events of apoptosis is a series of cellular morphology changes: nucleus condensation, cell shrinkage and rounding, cell surface blebbing, dynamic blebbing, apoptotic membrane protrusions and nucleus fragmentations and finally, the formation and release of apoptotic bodies. It is difficult to detect cellular changes in the early phase of apoptosis due to the subtle changes at this phase. In the current study, we induced apoptosis in He La cells with H2 O2 and used nuclear dye Hoechst 33258, mitochondria, lysosome and cytoplasmic protein specific aggregation-induced emission fluorogens(AIEgens), TPE-Ph-In, 2 M-DABS and BSPOTPE to successfully perform live cell multiplexed imaging to investigate early apoptosis cellular events. We showed the gradual dissipation of mitochondria membrane potential until it is nondetectable by TPE-Ph-In. Increased mitophagy detected by TPE-Ph-In and 2 M-DABS, condensed nucleus detected by Hoechst33258, increased permeability and/or reduced integrity of nuclear membrane, and increased intracellular vesicles detected by2 M-DABS are some of the early events of apoptosis. | Yabin Zhou Haixiang Liu Na Zhao Zhiming Wang Michael Z.Michael Ni Xie Ben Zhong Tang Youhong Tang | 2018 | Science China Chemistry2018，61，8： | 2 |
| 4 | Reinforce- ment effect of MAA on nano-CaCO3-filled EPDM vulcanizates and possible mechanism显示文摘 | Zhou Yabin Wang Shifeng Zhang Yinxi | 2006 | Journal of Polymer Science(part B) : Polymer Physics2006，44，8： | 1 |
| 5 | Reinforcement effect of MAA on nano-CaCO3 filled EPDM vulcanizates and possible mechanism显示文摘 | Zhou Yabin Wang Shifeng Zhang Yinxi | 2006 | Journal of Polymer Science (part B)2006，44，7： | 1 |
| 6 | History of high‐risk HPV and Pap test results in a large cohort of patients with invasive cervical carcinoma: Experience from the largest women’s hospital in China显示文摘 | Xiang Tao Christopher C. Griffith Xiangrong Zhou Zhiheng Wang Yabin Yan Zaibo Li Chengquan Zhao | 2015 | Cancer Cytopathology2015，，7： | 1 |
| 7 | Seroprevalence and risk factors of human cytomegalovirus infection in the eastern Chinese population显示文摘 | Ping Zhao Daoxin Ma Fuzhong Xue Chunyan Ji Shukang Wang Xiaomei Zhang Yabin Zhou Xiuping Yu | 2009 | Archives of Virology2009，，4： | 1 |
| 8 | Seroprevalence and risk factors of human cytomegalovirus infection in the eastern Chinese population显示文摘 | Ping Zhao Daoxin Ma Fuzhong Xue Chunyan Ji Shukang Wang Xiaomei Zhang Yabin Zhou Xiuping Yu | 2009 | Archives of Virology2009，，4： | 1 |
| 9 | Reinforcement Effect of MAA on Nano-CaCO3-filled EPDM Vulcanizates and Possible Mechanism显示文摘 | Zhou Yabin Wang Shifeng Zhang Yinxi | 2006 | Journal of Polymer Science Part B:Polymer Physics2006，44，8： | 1 |
| 10 | Reinforcement effect of MAA on nano-CaCO3 filled EPDM vulcanizates and possible mechanism显示文摘 | Yabin Zhou Shifeng Wang Yinxi Zhang Yong Zhang | 2006 | Journal of polymer science:Part B:polymer physics2006，44，： | 1 |
| 11 | Aggregation-induced Emission Fluorogen as Mammalian Cell Cytoplasmic Tracker with Long Retention Time and High Photo-stability显示文摘Fluorescent probes used for cell imaging are powerful tools in cell-based assays and research.In this study,we exhibited a water-soluble aggregation-induced emission fluorogen(AIEgen),BSPO-TPE,specifically stained cytoplasm in live cells and had an excellent photostability when compared to that of two widely used commercial fluorescent dyes.The long cytoplasm retention time of BSPO-TPE demonstrated its suitability as a live cell cytoplasm tracker. | ZHOU Yabin HUA Jin ZHANG Hong-ping TANG Youhong | 2021 | Chemical Research in Chinese Universities2021，37，1： | 0 |
| 12 | Fabrication of MgB_2 superconducting films by different methods显示文摘MgB 2 超导的电影成功地在单个水晶 MgO (111 ) 和 c-AL 2 由不同方法的 O 3 底层。当另外的二部电影由化学蒸汽免职制作了时,搏动的激光免职扔的电影是与 38.4K 的零抵抗 transistion 温度面向的 c 轴,电气泳动分别地是与 38 K 和 39 K 的零抵抗 transistion 温度粗糙的 c 轴。磁化磁滞现象大小产出批评当前的密度 J 在为薄电影并且为厚电影的 10 5A/ 厘米 2 的零块地里的 15K 的 10 7A/ 厘米 2 的 c 。为化学蒸汽免职扔的薄电影,在 10 K 的微波表面电阻被发现象 100 μ Ω 一样低;,它与 YBCO 的一部高质量的高温度的超导的薄电影的是可比较的。 | WANG Shufang ZHOU Yueliang ZHU Yabin CHEN Zhenghao LU Huibin YANG Guozhen | 2006 | Progress in Natural Science:Materials International2006，16，2： | 0 |
| 13 | 3 - 19 Preparation and Ultra-long PVP Assembly of Aligned Nanofibers显示文摘 | Zhou Ming Sun Jianrong Yao Cunfeng Gou Jie Sheng Yanbin Shen Tielong Zhu Yabin Pang Lilong Cui Minghuan Wang Zhiguang Xie Erqing | 2010 | IMP & HIRFL Annual Report2010，，1： | 0 |
| 14 | Establishment and application of national reference panels for SARS-CoV-2 antigen detection kit显示文摘To develop a national reference panel for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)antigen detection kit and establish a quality standard.The cultures of SARS-CoV-2 and other pathogens were collected to establish a national reference panel for SARS-CoV-2 antigen detection.The stability and homogeneity of the reference panel were evaluated.Based on World Health Organization(WHO)guidance and nucleic acid quantitative results,a quality standard reference panel was established.Currently,three generations of SARS-CoV-2 antigen national reference materials with batch numbers 370095–202001,370095–202202,and 370095–202203 have been successfully established.These national reference panels comprised 8 positive samples,20 negative samples,1 repetitive sample,and 1 lower detection limit sample.The stability and homogeneity of the reference panel meet the requirements.The quality standards are as follows:the positive and negative coincidence rates are 8/8 and 20/20,respectively.The 10 test results of the medium and low-concentration repetitive reference materials should be positive,and the color rendering should be uniform(or the coefficient of variance should not be higher than 20.0%).The lower detection limit should be at least 5×105 U/mL(equivalent to copies/mL),and higher concentrations above the lower detection limit must be positive.A national reference panel for the SARS-CoV-2 antigen detection kit has been established.As the standard of SARS-CoV-2 antigen reagents,the reference panel has played a crucial role in the pre-marketing quality evaluation and post-marketing quality supervision in China. | Tingting Ma Donglai Liu Keliang Lyu Tingting Gao Dawei Shi Lanqing Zhao Shu Shen Yabin Tian Sihong Xu Haiwei Zhou | 2023 | Biosafety and Health2023，5，6： | 0 |
| 15 | Approaches to improving critical current density of YBCO superconducting thick films deposited by electrophoresis显示文摘Fabrication of YBCO superconducting thick films by electrophoresis has been widely reported. However, the value of critical current density (Jc) is far from what the practical application requires. The superconducting thick films are obtained using top-seeded melt growth method with different proportional Y2BaCuO5 (Y211) addition. High-pressure oxygen has been used in the annealing process to improve the uniformity of the oxygen content in the superconducting thick films. When 40mol% Y211 powder is added to the YBa2Cu3O7-d (Y123) powder, Jc for the superconducting thick film by approaches described above reaches 7.008?03 A/cm2 (77 K, 0 T), which is greater than the value ever reported. | ZHU Yabin1,2, ZHOU Yueliang2, LIU Zhen2, WANG Shufang2, CHEN Zhenghao2, L?Huibin2, YANG Guozhen2, XIAO Ling3, REN Hongtao3, JIAO Yulei3 & ZHENG Minghui3 1. Department of Physics, College of Science, Beijing Jiaotong University, Beijing 100044, China 2. Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China 3. General Research Institute for Nonferrous Metals, Beijing 100088, China Correspondence should be addressed to Zhu Yabin (email: ybzhu@science.njtu.edu.cn) | 2004 | Science China(Physics,Mechanics & Astronomy)2004，47，2： | 0 |
| 16 | Culture of Eriocheir sinensis Larvae in Ponds in Greenhouse显示文摘A total of 15 pools were selected from a greenhouse, and they were randomly and evenly divided into three groups.In each group, one stocking density of Eriocheir sinensis was arranged. The results showed that after 45-d culture, the crab number per kilogram reached about 300 with survival rate of about 20%; the initial stocking density had significantly effect on the body size of E. sinensis on sell, instead on the survival rate of E. sinensis larvae. Therefore, it is feasible to conduct the culture of E. sinensis larvae in a pool in greenhouse. | Guoliang RUAN Liangcheng HU Hai ZHOU Dawen MA Yabin TANG | 2016 | Agricultural Science & Technology2016，17，3： | 0 |
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