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| 1 | Polymorphisms of three gene-derived STS on pig chromosome 13q41 are associated with susceptibility to enterotoxigenic Escherichia coli F4ab/ac in pigs显示文摘Neonatal diarrhea caused by enterotoxigenic Escherichia coli(ETEC)F4 is a common and serious disease,resulting in significant economical loss in the pig industry.The locus encoding ETEC F4 receptor has been mapped to pig chromosome(SSC)13q41,and one of the most significantly linked markers is S0075.In this study,we selected three genes including SLC12A8,MYLK and KPNA1 from a chromosomal region flanking S0075 on SSC13 to develop pig specific sequence tagged sites(STS). Seven single nucleotide polymorphisms were identified in the three pig STS using DNA of four full-sib susceptible and resistant animals in a White Duroc×Erhualian intercross.All grandparents,parents and 755 offspring in the intercross were genotyped for three polymorphisms,including SLC12A8 g.159A>G,MYLK g.1673A>G and KPNA1 g.306A>G.Family-based transmission disequilibrium test(TDT) revealed that all polymorphisms and the corresponding haplotypes are significantly associated with ETEC F4ab/ac(especially F4ac)brush border adhesion phenotypes,indicating that these polymor- phism are in linkage disequlibrium with causal mutation(s)of the gene encoding ETEC F4ab/ac receptor. Our results strengthen the evidence for the involvement of SSC13q41 in high acquiring risk of ETEC F4ab/ac infection,and provide novel polymorphic markers for fine mapping of the ETEC F4ab/ac receptor locus. | HUANG Xiang 1 ,REN Jun 1 ,YAN XueMing 1,2 ,PENG QiuLing 1 ,TANG Huan 1 ,ZHANG Bo 1 ,JI HuaYuan 1 , YANG ShuJin1&HUANG LuSheng 1 1Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China,Jiangxi Agricultural University, Nanchang 330045,China 2Department of Biology,Nanchang University of Science and Technology,Nanchang 330038,China | 2008 | Science China(Life Sciences)2008，51，7： | 8 |
| 2 | Aquaporin 5 is degraded by autophagy in diabetic submandibular gland显示文摘Autophagy is a catabolic process which is involved in the development of many diseases including diabetes mellitus and its complications. Hyposalivation is a common complication of diabetes mellitus, whereas its mechanism remains unclear. Here, we observed that the stimulated salivary flow rate of SMG was significantly decreased in db/db mice, a diabetic mice model. The expressions of aquaporin 5(AQP5), a water channel protein, were decreased, whereas the m RNA level of AQP5 was increased in SMGs of both diabetic patients and mice. Under transmission electron microcope, more autophagosomes were detected in diabetic SMGs. Expressions of autophagy related proteins LC3 II, Beclin-1 and ATG5 were increased, meanwhile autophagy substrate p62 was decreased in SMGs of diabetic patients and mice, indicating that autophagy was activated in diabetic SMG.Double immunofluorescence staining showed that the colocalization of AQP5 and LC3 was increased in SMGs of diabetic mice.In cultured SMG-C6 cells, high glucose(HG), but not high osmotic pressure, reduced AQP5 protein expression and induced autophagy. Moreover, inhibition of autophagy by 3-methyladenin, an autophagy inhibitor, or by autophagy-related gene 5 siRNA, decreased HG-induced AQP5 reduction in SMG-C6 cells. Additionally, the expression of p-p85, p-Akt and p-mTOR were decreased in HG-treated SMG-C6 cells. Pretreatment with 740 Y-P, a PI3 K agonist, significantly suppressed HG-induced autophagy and AQP5 degradation. Taken together, these results indicate that autophagy plays a crucial role in AQP5 degradation in diabetic SMG via PI3 K/Akt/mTOR signaling pathway, which contributes to the dysfunction of diabetic SMG. Our study provides a novel mechanism of diabetic hyposalivation. | Yan Huang Xijin Shi Qianying Mao Yan Zhang Xin Cong Xueming Zhang Zhejing Zhang Liling Wu Ruolan Xiang Guangyan Yu | 2018 | Science China(Life Sciences)2018，61，9： | 5 |
| 3 | Architecture of type VI secretion system membrane core complex显示文摘Dear Editor, Type VI secreti on system (T6SS) adopts a con tact-dependent secretion mechanism to deliver various lethal effectors into prokaryotic or eukaryotic cells.1,2 Due to its antibacterial and anti-eukaryotic activity, T6SS provides a powerful weapon for bacteria to kill their competitors and attackers.2 T6SS has been show n to resemble a bacteriophage tail-like nano machine of more than 13 components. | Meng Yin Zhaofeng Yan Xueming Li | 2019 | Cell Research2019，29，3： | 5 |
| 4 | DYNAMIC CHARACTERISTIC ANALY-SIS OF PRECISE LONG STROKE LINEAR MOTOR WITH AIR-BEARING IN OPTICAL LITHOGRAPHY显示文摘Dynamic characteristic is presented by identifying the model and the dynamic parameters of a precise long stroke linear motor (PLSLM) with the air-bearing in optical lithography.The PLSLM is supported by air-bearing on the stator,and is driven by on-board two large linear motors in a cross-configuration.Firstly,a model of the PLSLM is established by finite element method (FEM). Secondly,based on the model,the natural frequencies and model shapes are discussed.And the contribution of each active mode is evaluated by computing the modal participation factors (MPF), which indicates the major vibration direction.Furthermore,by the experimental modal analysis,the experimental results are in agreement with simulation results,which it is sure that the FEM is reasonable.What′s more,comparing with the effects on the frequency due to the air-bearing stiffness, the relations of the natural frequencies with the air-bearing stiffness are found.It is found that the frequency response curve is fluctuant with the air-bearing stiffness in each direction.Finally,it is conclusion that the natural frequency of the PLSLM is largely affected by the air-bearing stiffness variety.This research is contributed to the dynamic characteristics resulted from the air-bearing stiffness.Further work will include better optimization on the dynamic parameter in the controller design through the control algorithm for the precise long stroke motor. | CHEN Xuedong YU Xianzhong HE Xueming YAN Tianhong | 2008 | Chinese Journal of Mechanical Engineering2008，21，2： | 4 |
| 5 | Clinical curative effect of fuzi-cake-separated moxibustion for preventing dysuria after operation for lower limb fracture显示文摘OBJECTIVE: To assess the clinical curative effect of fuzi-cake-separated moxibustion at Zhongji(CV 3)and Guanyuan(CV 4) for preventing dysuria after internal fixation of lower limb fractures.METHODS: Sixty patients conforming to the inclusion standards were randomly divided into a treatment group(n=30) and a control group(n=30).Fuzi-cake-separated moxibustion was performed at Guanyuan(CV 4) and Zhongji(CV 3), 20 min at a time, twice a day, for 3 days before operation in the treatment group. No fuzi-cake-separated moxibustion was performed in the control group. After treatment, the score for symptoms of first urination, urinary time, urinary volume, 24 h remaining urinary volume, incidence of uroschesis, and rate of controlling dysuria were compared to evaluate the curative effect of preventing post-operative dysuria.RESULTS: The score for symptoms of first urination,24 h remaining urinary volume(maximum 120 m L vs 250 m L, and less than 10 m L in 24 cases vs 15 cases), and the rate of controlling dysuria(83.34% vs30%) were significantly better(P<0.05, P<0.05, and P<0.001, respectively) in the treatment compared with the control group. There was no statistical difference(P>0.05) between the two groups in first post-operative urinary time, urinary volume, or incidence of 24 h uroschesis.CONCLUSION: Fuzi-cake-separated moxibustion at Zhongji(CV 3) and Guanyuan(CV 4) can better prevent post-operative dysuria, effectively promote the functional restoration of the urinary bladder,and control the incidence of post-operative dysuria. | Yan Yue Lijun Tao Jianqiao Fang Qi Xie Shaofeng He Chunxia Huang Xueming Yang | 2014 | Journal of Traditional Chinese Medicine2014，34，5： | 4 |
| 6 | Development of a CLDN18.2-targeting immuno-PET probe for non-invasive imaging in gastrointestinal tumors显示文摘Claudin18.2(CLDN18.2)is a tight junction protein that is overexpressed in a variety of solid tumors such as gastrointestinal cancer and oesophageal cancer.It has been identified as a promising target and a potential biomarker to diagnose tumor,evaluate efficacy,and determine patient prognosis.TST001 is a recombinant humanized CLDN18.2 antibody that selectively binds to the extracellular loop of human Claudin18.2.In this study,we constructed a solid target radionuclide zirconium-^(89)(^(89)Zr)labled-TST001 to detect the expression of in the human stomach cancer BGC823CLDN18.2 cell lines.The[^(89)Zr]Zr-desferrioxamine(DFO)-TST001 showed high radiochemical purity(RCP,>99%)and specific activity(24.15±1.34 GBq/mmol),and was stable in 5%human serum albumin,and phosphate buffer saline(>85%RCP at 96 h).The EC_(50) values of TST001 and DFO-TST001 were as high as 0.413±0.055 and 0.361±0.058 nM(P>0.05),respectively.The radiotracer had a significantly higher average standard uptake values in CLDN18.2-positive tumors than in CLDN18.2-negative tumors(1.11±0.02 vs.0.49±0.03,P=0.0016)2 days post injection(p.i.).BGC823CLDN18.2 mice models showed high tumor/muscle ratios 96 h p.i.with[^(89)Zr]Zr-DFO-TST001 was much higher than those of the other imaging groups.Immunohistochemistry results showed that BGC823CLDN18.2 tumors were highly positive(+++)for CLDN18.2,while those in the BGC823 group did not express CLDN18.2().The results of ex vivo biodistribution studies showed that there was a higher distribution in the BGC823CLDN18.2 tumor bearing mice(2.05±0.16%ID/g)than BGC823 mice(0.69±0.02%ID/g)and blocking group(0.72±0.02%ID/g).A dosimetry estimation study showed that the effective dose of[^(89)Zr]Zr-DFO-TST001 was 0.0705 mSv/MBq,which is within the range of acceptable doses for nuclear medicine research.Taken together,these results suggest that Good Manufacturing Practices produced by this immuno-positron emission tomography probe can detect CLDN18.2-overexpressing tumors. | Yan Chen Xingguo Hou Dapeng Li Jin Ding Jiayue Liu Zilei Wang Fei Teng Hongjun Li Fan Zhang Yi Gu Steven Yu Xueming Qian Zhi Yang Hua Zhu | 2023 | Journal of Pharmaceutical Analysis2023，13，4： | 2 |
| 7 | Identification of EGF as an Important Regulator for Promoting CYP3A4 Expression in Human Embryonic Stem Cell-Derived Hepatocytes Using TALEN-Based Gene Targeting显示文摘Functional human hepatocytes are one of the most significant tools for studying drug absorption,distribution,metabolism and excretion/toxicity (ADME/Tox),especially for applications in preclinical drug development (Sahi et al.,2010;Godoy et al.,2013).They provide the closest in vitro model to the human liver and the only model that mimics the drug metabolic profiles found in vivo.However,these cells lose their metabolic function rapidly and dramatically during the in vitro culture process,which largely hinders their wider application in drug development (Sahi et al.,2010;Godoy et al.,2013).To overcome this obstacle,it is important to regulate the activities of key genes which are responsible for the detoxification metabolic function of human hepatocytes. | Ming YinaCollege of Veterinary Medicine Jilin University Huan Yang Xiaohua Su Ziyi Li Zhanpeng Yue Xueming Zhang Da Sun Yan Shi Dexue Li | 2014 | Journal of Genetics and Genomics2014，41，6： | 2 |
| 8 | Silencing myostatin gene by RNAi in sheep embryos显示文摘 | Dayun Tang Huabin Zhu Jianmin Wu Hanzhong Chen Yan Zhang Xueming Zhao Xiaoliang Chen Weihua Du Dong Wang Xiukun Lin | 2012 | Journal of Biotechnology2012，，3： | 2 |
| 9 | Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy显示文摘Major challenges for cancer treatment are how to effectively eliminate primary tumor and sufficiently induce immunogenic cell death(ICD)to provoke a robust immune response for metastasis control.Here,a self-assembled cascade bioreactor was developed to improve cancer treatment with enhanced tumor penetration and synergistic therapy of starvation,chemodynamic(CDT)and photothermal therapy.Ultrasmall Fe S-GOx nanodots were synthesized with glucose oxidase(GOx)as template and induced by paclitaxel(PTX)to form self-assembling Fe S-GOx@PTX(FGP)via hydrophobic interaction.After accumulated at tumor sites,FGP disassembles to smaller Fe S-GOx for enhanced deep tumor penetration.GOx maintains high enzymatic activity to catalyze glucose with assistant of oxygen to generate hydrogen peroxide(H2O2)as starvation therapy.Fenton reaction involving the regenerated H_(2)O_(2) in turn produced more hydroxyl radicals for enhanced CDT.Following near-infrared laser at 808 nm,FGPs displayed pronounced tumor inhibition in vitro and in vivo by the combination therapy.The consequent increased exposure to calreticulin amplified ICD and promoted dendritic cells maturation.In combination with anti-CTLA4 checkpoint blockade,FGP can absolutely eliminate primary tumor and avidly inhibit distant tumors due to the enhanced intratumoral infiltration of cytotoxic T lymphocytes.Our work presents a promising strategy for primary tumor and metastasis inhibition. | Hao Ren Jiahui Yong Qingqing Yang Zhen Yang Zhangya Liu Yan Xu Hao Wang Xing Jiang Wenjun Miao Xueming Li | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 2 |
| 10 | Identification of differentially expressed proteins between bull X and Y spermatozoa显示文摘 | Xiaoli Chen Huabin Zhu Chengjiang Wu Weidong Han Haisheng Hao Xueming Zhao Weihua Du Tong Qin Yan Liu Dong Wang | 2012 | Journal of Proteomics2012，，： | 1 |
| 11 | Silencing myostatin gene by RNAi in sheep embryos显示文摘 | Dayun Tang Huabin Zhu Jianmin Wu Hanzhong Chen Yan Zhang Xueming Zhao Xiaoliang Chen Weihua Du Dong Wang Xiukun Lin | 2012 | Journal of Biotechnology2012，，3： | 1 |
| 12 | Soil organic carbon changes in particle-size fractions following cultivation of Black soils in China显示文摘 | Aizhen Liang Xueming Yang Xiaoping Zhang Neil McLaughlin Yan Shen Wenfeng Li | 2009 | Soil & Tillage Research2009，，1： | 1 |
| 13 | Multiplex detection of transgenic maize by microdroplet PCR combined with capillary gel electrophoresis显示文摘With tremendous progress in the commercialization of genetically modified(GM)organisms(GMOs),the safety of GM foods has become a heated topic of debate,and many countries or organizations have adopted the GMO labeling systems[1].GM foods were labeled at 0.9%threshold in the European Union,1%in Brazil,3%in Korea,and 5%in Japan[1,2].In China,all GMOs and GM products are required to be labeled according to the Department of Agriculture,and the main purpose of labeling is to protect consumers’right to know and choose[2]. | Xiao Zhao Qi Zhang Xueming Tang Yan Xu Yanan Du Binan Zhao Xiaori Fan Xiaoxia Zhang Wanjing Zhang Wenjing Wu Kai Zhao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，5： | 1 |
| 14 | Susceptibility towards enterotoxigenic Escherichia coli F4ac diarrhea Is governed by the MUC13 gene in pigs显示文摘 | Ren Jun Yan Xueming | 2012 | PLoS One2012，7，44： | 1 |
| 15 | Dietary supplementation with purified mulberry ( Morus australis Poir) anthocyanins suppresses body weight gain in high-fat diet fed C57BL/6 mice显示文摘 | Tao Wu Xueming Qi Yan Liu Jun Guo Ruiyu Zhu Wei Chen Xiaodong Zheng Ting Yu | 2013 | Food Chemistry2013，，1： | 1 |
| 16 | Susceptibility Towards Enterotoxigenic Escherichiacoli F4 ac Diarrhea is Governed by the MUC13 Gene in Pigs显示文摘 | Jun Ren Xueming Yan Huashui Ai | | 0，，09： | 1 |
| 17 | Ultrasonic-assisted enzymatic degradation of cholecterol in egg yolk 显示文摘 | Yan Sun Hailing Yang Xueming Zhong | 2011 | Innovative Food Science and Emerging Technologies2011，12，： | 1 |
| 18 | Dietary supplementation with purified mulberry(Morus australis Poir)anthocyanins suppresses body weight gain in high-fat diet fed C57BL/6 mice显示文摘 | WU Tao QI Xueming LIU Yan GUO Jun ZHU Ruiyu CHEN Wei ZHENG Xiaodong YU Ting | 2013 | Food Chemistry2013，141，1： | 1 |
| 19 | Simulation of the water level influence on the difference within the water-tube tiltmeter in Shuangyang Lake显示文摘This article analyzes the relationship between the water level and the water-tube tilting in Shuangyang lake,based on the differential deformation features reflected by the NS and EW components of the water-tube tiltmeter.The results show a good spatiotemporal consistency between the variation of water level and the NS tilt component,which is considered to be affected by the magnitude and duration of the water level variation in Shuangyang Lake.The article uses Landsat remote sensing image data to extract the water boundary of Shuan-gyang Lake,and takes advantage of the finite element numerical simulation method to build three-dimensional models for different geological structural conditions of the Shuangyang seismostation.The simulation results show that when the underground medium is granite,the effect of water level variation on the vertical displacement of the surface is non-directional.With a 50-m soil layer in Model 2,the simulated NS tilt variation is equivalent to the actual observed water-tube tiltmeter NS component when the water level variation is 0.44 m and 0.8m.When the variation of water level reaches 2.0m,the simulation result of the NS component is 79.6 ms,which is slightly larger than the observed result of 60.32 ms.However,the simulation results show that the variation of the EW component is significantly smaller than that of the NS one.Due to the fact that the Shuan-gyang lake is long in the NS direction and short in the EW direction,the existence of the soil layer tends to generate ground deformation along the NS direction in the vicinity of the lake after the increase of water level,thereby resulting in the difference of the ground deformation in the two directions. | Chong Yue Chuncheng Tang Wei Yan Xiaodong Pan Xueme Li Yuwen Tan Zongfeng Zhang Tianlong Yu | 2021 | Earthquake Research Advances2021，1，2： | 1 |
| 20 | Autonomic reinnervation and functional regeneration in autologous transplanted submandibular glands in patients with severe keratoconjunctivitis sicca显示文摘Autologous submandibular gland(SMG) transplantation has been proved to ameliorate the discomforts in patients with severe keratoconjunctivitis sicca. The transplanted glands underwent a hypofunctional period and then restored secretion spontaneously.This study aims to investigate whether autonomic nerves reinnervate the grafts and contribute to the functional recovery, and further determine the origin of these nerves. Parts of the transplanted SMGs were collected from the epiphora patients, and a rabbit SMG transplantation model was established to fulfill the serial observation on the transplanted glands with time. The results showed that autonomic nerves distributed in the transplanted SMGs and parasympathetic ganglionic cells were observed in the stroma of the glands. Low-dense and unevenly distributed cholinergic axons, severe acinar atrophy and fibrosis were visible in the patients' glands 4–6 months post-transplantation, whereas the cholinergic axon density and acinar area were increased with time. The acinar area or the secretory flow rate of the transplanted glands was statistically correlated with the cholinergic axon density in the rabbit model, respectively. Meanwhile, large cholinergic nerve trunks were found to locate in the temporal fascia lower to the gland, and sympathetic plexus concomitant with the arteries was observed both in the adjacent fascia and in the stroma of the glands. In summary, the transplanted SMGs are reinnervated by autonomic nerves and the cholinergic nerves play a role in the morphological and functional restoration of the glands. Moreover, these autonomic nerves might originate from the auriculotemporal nerve and the sympathetic plexus around the supplying arteries. | Xueming Zhang Ningyan Yang Xiaojing Liu Jiazeng Su Xin Cong Liling Wu Yan Zhang Guangyan Yu | 2018 | International Journal of Oral Science2018，10，2： | 1 |
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