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| 1 | A Verifier-based Password-authenticated Key Exchange Protocol via Elliptic Curves显示文摘 | Yang Junhan Cao Tianjie | | 0，，02： | 1 |
| 2 | Provably Secure Three-party Password Authenticated Key Exchange Protocol in the Standard Model显示文摘 | Yang Junhan Cao Tianjie | 2012 | Journal of Systems and Software2012，85，2： | 1 |
| 3 | Lipidomic analyses reveal enhanced lipolysis in planthoppers feeding on resistant host plants显示文摘The brown planthopper(BPH)(Nilaparvata lugens St?l)is a highly destructive pest that seriously damages rice(Oryza sativa L.)and causes severe yield losses.To better understand the physiological and metabolic mechanisms through which BPHs respond to resistant rice,we combined mass-spectrometry-based lipidomics with transcriptomic analysis and gene knockdown techniques to compare the lipidomes of BPHs feeding on either of the two resistant(NIL-Bph6 and NIL-Bph9)plants or a wild-type,BPH susceptible(9311)plant.Insects that were fed on resistant rice transformed triglyceride(TG)to phosphatidylcholine(PC)and digalactosyldiacylglycerol(DGDG),with these lipid classes showing significant alterations in fatty acid composition.Moreover,the insects that were fed on resistant rice were characterized by prominent expression changes in genes involved in lipid metabolism processes.Knockdown of the NlBmm gene,which encodes a lipase that regulates the mobilization of lipid reserves,significantly increased TG content and feeding performance of BPHs on resistant plants relative to dsGFP-injected BPHs.Our study provides the first detailed description of lipid changes in BPHs fed on resistant and susceptible rice genotypes.Results from BPHs fed on resistant rice plants reveal that these insects can accelerate TG mobilization to provide energy for cell proliferation,body maintenance,growth and oviposition. | Xiaohong Zheng Yeyun Xin Yaxin Peng Junhan Shan Ning Zhang Di Wu Jianping Guo Jin Huang Wei Guan Shaojie Shi Cong Zhou Rongzhi Chen Bo Du Lili Zhu Fang Yang Xiqin Fu Longping Yuan Guangcun He | 2021 | Science China(Life Sciences)2021，64，9： | 1 |
| 4 | PhaseFIT:live-organoid phase-fluorescent image transformation via generative AI显示文摘Organoid models have provided a powerful platform for mechanistic investigations into fundamental biological processes involved in the development and function of organs.Despite the potential for image-based phenotypic quantification of organoids,their complex 3D structure,and the time-consuming and labor-intensive nature of immunofluorescent staining present significant challenges.In this work,we developed a virtual painting system,PhaseFIT(phase-fluorescent image transformation)utilizing customized and morphologically rich 2.5D intestinal organoids,which generate virtual fluorescent images for phenotypic quantification via accessible and low-cost organoid phase images.This system is driven by a novel segmentation-informed deep generative model that specializes in segmenting overlap and proximity between objects.The model enables an annotation-free digital transformation from phase-contrast to multi-channel fluorescent images.The virtual painting results of nuclei,secretory cell markers,and stem cells demonstrate that PhaseFIT outperforms the existing deep learning-based stain transformation models by generating fine-grained visual content.We further validated the efficiency and accuracy of PhaseFIT to quantify the impacts of three compounds on crypt formation,cell population,and cell stemness.PhaseFIT is the first deep learning-enabled virtual painting system focused on live organoids,enabling large-scale,informative,and efficient organoid phenotypic quantification.PhaseFIT would enable the use of organoids in high-throughput drug screening applications. | Junhan Zhao Xiyue Wang Junyou Zhu Chijioke Chukwudi Andrew Finebaum Jun Zhang Sen Yang Shijie He Nima Saeidi | 2023 | Light(Science & Applications)2023，12，12： | 0 |
| 5 | Training needs in intravenous infusion care for children in China:A cross-sectional study显示文摘Aim:This study aims to evaluate demands for general and pharmacological knowledge and training related to intravenous(IV)therapy among pediatric nurses.Materials and Methods:This multicentric cross‑sectional study involved 12,707 pediatric nurses from 100 hospitals in China.A questionnaire was used to assess information about IV drug administration training received,and the demands for pharmacology‑related knowledge,and corresponding methods of acquisition.A generalized linear model using Logit link function was employed to assess relationships between factors and multivariate analysis was conducted.Results:More than 99%of participants showed their training demands for IV therapy training.Pediatric nurses’demands and methods for acquiring IV therapy knowledge and the knowledge related to IV therapy pharmacology have significant differences in social demographic factors,previous knowledge,whether they have received training or not,and other factors(all P<0.05).Received a needlestick injury in the past month(P=0.007)and knowledge acquired through in‑hospital or out‑of‑hospital training(P=0.039)were factors that reduced the demands for methods to acquire further pharmacology‑related knowledge of IV therapy.Working in internal medicine(P=0.025)and not having experienced a needlestick injury in the past year(P=0.007)reduced the demands for IV therapy knowledge.Attended hospital(P=0.007)or departmental meetings(P=0.009),being pediatric primary nurses(P=0.044),and studied special IV guidelines(P=0.006)reduced the desire for methods to acquire IV therapy knowledge.Conclusion:There was a high demand for greater general and pharmacological knowledge related to IV therapy among pediatric nurses.Resources should be coordinated to provide ongoing training to nurses to improve the quality of IV care. | Jingjing LI Jiaxin FANG Liu HAN Junhan YANG Xueyan FAN Dahua ZHANG | 2023 | Journal of Integrative Nursing2023，5，2： | 0 |
| 6 | Gene-like Precise Construction of Functional DNA Materials显示文摘CONSPECTUS:Developing a new system of material chemistry is an important molecular foundation for the design and preparation of functional materials,which resembles the preparation of raw materials such as bricks for constructing a building that determines the quality of the building.However,precisely controlling the synthesis processes and structures of functional materials always remains a grand challenge.It is expected to propose a paradigm of“gene-like”precise construction to realize the rational synthesis of functional materials,i.e.,a“structure-function-application”principle for the precise construction.As the core genetic material of life,the DNA molecule is a bioactive macromolecule that can accurately encode genetic information and also can act as the generic molecular building block for the precise construction of functional materials.In this Account,we describe our work on the design and construction of functional DNA materials,to illustrate the principle of“gene-like”construction of functional DNA materials.The DNA molecule shows the unique advantages in the“gene-like”construction of functional materials,mainly including the precise arrangement of bases(monomers),the controllable design and assembly of structure,the precise transmission of sequence information,and customization of function.First,the number and sequence of four deoxynucleotide monomers that constitute the DNA strand can be rationally designed and accurately synthesized.Second,the sequence information on DNA endows the precise and efficient assembly of DNA molecules and ensures the precise regulation on the specific biological functions of functional DNA materials.Third,the functions of DNA materials can couple with the biological environments,so as to achieve the predetermined applications.Based on the scale of the constructed DNA materials,we categorize DNA materials into three classes:molecular scale,nanoscale,and macroscale.At the molecular scale,the representative material is branched DNA-based materials;the construction strategies mainly include targettriggered polymerization,enzymatic extension,and hybrid coupling;the applications mainly include the nonenzymatic detection of base mutation,the construction of artificial cells for the study of compartmentalization and the confinement effect,and the regulation of optical and antibacterial properties of supernanoclusters.At the nanoscale,the representative material is the DNA nanocomplex;the construction strategies mainly include hybridization with polymer,small molecule,and metal ions;the applications mainly include gene drug delivery and luminescence bioimaging.At the macroscale,the representative material is the DNA hydrogel;the construction strategies mainly include double-rolling circle amplification(double-RCA),multistage-RCA,and chemical cross-linking;the applications mainly include cell isolation,cell delivery,antibacterial agents,and self-healing electric circuits.Based on the“gene-like”paradigm,we expect to develop a wider variety of functional DNA materials by the precise regulation of DNA assembly behaviors and topological structures.We further envision that our work on the design,synthesis,and applications of functional DNA materials is a typical paradigm for the“gene-like”precise construction of functional materials and hopefully will promote the development of materials genome. | Chi Yao Yuwei Xu Pin Hu Junhan Ou Dayong Yang | 2022 | Accounts of Materials Research2022，3，1： | 0 |
| 7 | Biomimetic“Gemini nanoimmunoregulators”orchestrated for boosted photoimmunotherapy by spatiotemporally modulating PD-L1 and tumor-associated macrophages显示文摘A novel strategy of not only stimulating the immune cycle but also modulating the immunosuppressive tumor microenvironment is of vital importance to efficient cancer immunotherapy.Here,a new type of spatiotemporal biomimetic“Gemini nanoimmunoregulators”was engineered to activate robust systemic photoimmunotherapy by integrating the triple-punch of amplified immunogenic cell death(ICD),tumor-associated macrophages(TAMs)phenotype reprogramming and programmed cell death ligand 1(PD-L1)degradation.The“Gemini nanoimmunoregulators”PM@RM-T7 and PR@RM-M2 were constructed by taking the biocompatible mesoporous polydopamine(mPDA)as nanovectors to deliver metformin(Met)and toll-like receptor 7/8 agonist resiquimod(R848)to cancer cells and TAMs by specific biorecognition via wrapping of red blood cell membrane(RM)inlaid with T7or M2 peptides.mPDA/Met@RM-T7(abbreviated as PM@RM-T7)was constructed to elicit an amplified in situ ICD effect through the targeted PTT and effectively stimulated the anticancer immunity.Meanwhile,PD-L1 on the remaining cancer cells was degraded by the burst metformin to prevent immune evasion.Subsequently,mPDA/R848@RM-M2(abbreviated as PR@RM-M2)specifically recognized TAMs and reset the phenotype from M2 to M1 state,thus disrupting the immunosuppressive microenvironment and further boosting the function of cytotoxic T lymphocytes.This pair of sister nanoimmunoregulators cooperatively orchestrated the comprehensive anticancer activity,which remarkably inhibited the growth of primary and distant 4T1 tumors and prevented malignant metastasis.This study highlights the spatiotemporal cooperative modalities using multiple nanomedicines and provides a new paradigm for efficient cancer immunotherapy against metastatic-prone tumors. | Honglin Huang Ningxi Li Xiaodan Wei Qingzhi Li Junhan Guo Geng Yang Hong Yang Lulu Cai Yiyao Liu Chunhui Wu | 2024 | Acta Pharmaceutica Sinica B2024，14，3： | 0 |
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