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| 1 | Dynamic Analyses of PrP and PrP^(Sc) in Brain Tissues of Golden Hamsters Infected With Scrapie Strain 263K Revealed Various PrP Forms显示文摘Objective To expatiate dynamic changes in hamsters infected with scrapie strain 263K,to observe the presence and aggravation of various forms of PrP and PrP^sc during incubation period,and to probe primarily the relationship between the onset of clinic manifestations and the presence of different PrP^sc forms. Methods Hamster-adapted scrapie strain 263K was intracerebrally inoculated into hamsters. Different forms of PrP and PrP^sc were monitored dynamically by Western blot and immuno-histochemical assays. The presence of scrapie-associated fibril (SAF) was assayed by election microscopy analysis (EM) and immuno-golden EM.Results PrP^sc was initially detected in the brain tissues of the animals in 20 days post-inoculation by immunohistochemistry and 40 days with Western blot. Quantitative evaluations revealed that the amounts of PrP and PrP^sc in brain tissues increased along with the incubation.Several high and low molecular masses of PrP were seen in the brains of the long-life span infected animals. Deglycosylation assays identified that the truncated PrP in the infected brains showed similar glycosylation patterns as the full-length PrP. The presence of short fragments was seemed to relate with the onset of clinical conditions. Conclusion These results indicate that infectious agents exist and accumulate in central nerve system prior to the onset of the illness. Various molecular patterns of PrPs~ may indwell in brain tissues during the infection. | JIAN-MEIGAO CHENGAO JUNHAN XIAO-BOZHOU XIN-LIXIAO JINZHANG LANCHEN BAO-YUNZHANG TAOHONG XIAO-PINGDONG | 2004 | Biomedical and Environmental Sciences2004，17，1： | 14 |
| 2 | Bph30confers resistance to brown planthopperby fortifying sclerenchyma in rice leaf sheaths显示文摘Phloem-feeding insects cause massive losses in agriculture and horticulture.Host plant resistance to phloem-feeding insects is often mediated by changes in phloem composition,which deter insect settling and feeding and decrease viability.Here,we report that rice plant resistance to the phloem-feeding brown planthopper(BPH)is associated with fortification of the sclerenchyma tissue,which is located just beneath the epidermis and a cell layer or two away from the vascular bundle in the rice leaf sheath.We found that BPHs prefer to feed on the smooth and soft region on the surface of rice leaf sheaths called the long-cell block.We identified Bph30 as a rice BPH resistance gene that prevents BPH stylets from reaching the phloem due to the fortified sclerenchyma.Bph30 is strongly expressed in sclerenchyma cells and enhances cellulose and hemicellulose synthesis,making the cell walls stiffer and sclerenchyma thicker.The structurally fortified sclerenchyma is a formidable barrier preventing BPH stylets from penetrating the leaf sheath tissues and arriving at the phloem to feed.Bph30 belongs to a novel gene family,encoding a protein with two leucine-rich domains.Another member of the family,Bph40,also conferred resistance to BPH.Collectively,the fortified sclerenchyma-mediated resistance mechanism revealed in this study expands our understanding of plant-insect interactions and opens a new path for controlling planthoppers in rice. | Shaojie Shi Huiying Wang Lingyun Nie Di Tan Cong Zhou Qian Zhang YiLi Bo Du Jianping Guo Jin Huang DiWu Xiaohong Zheng Wei Guan Junhan Shan Lili Zhu Rongzhi Chen Longjian Xue Linda L.Walling Guangcun He | 2021 | Molecular Plant2021，14，10： | 7 |
| 3 | Comparison Study on Clinical and Neuropathological Characteristics of Hamsters Inoculated With Scrapie Strain 263K in Different Challenging Pathways显示文摘Objective To understand the infectious characteristics of a hamster-adapted scrapie strain 263K with five different routes of infection including intracerebral (i.c.), intraperitoneal (i.p.),intragastrical (i.g.), intracardiac and intramuscular (i.m.) approaches. Methods Hamsters were infected with crude- or fine-prepared brain extracts. The neuropathological changes, PrP^sc deposits,and patterns of PK-resistant PrP were analyzed by HE stain, immunohistochemistry (HC) assay and Western blot. Reactive gliosis and neuron loss were evaluated by glial fibfillary acidic protein (GFAP) and neuron specific enolase (NSE) specific IHC.Results The animals inoculated in i.m. and i.p.ways with crude PrP^sc extracts showed clinical signs at the average incubation of 69.2±2.8 and 65.5±3.9 days. Inoculation in i.c. and intracardiac ways with fine PrP^sc extracts (0.00035g) caused similar,but relative long incubation of around 90 days. Only one out of eight hamsters challenged in i.g way with low dosage (0.01g) became ill after a much longer incubation (185d), while all animals (4/4)with high dosage (0.04g) developed clinical signs 105 days postirrfection. The most remarkable spongiform degeneration and PrP^sc deposits were found in brain stem among the five challenge groups generally. The number of GFAP-positive astrocytes increased distinctly in brain stems in all infection groups, while the number of NSE-positive cells decreased significantly in cerebrum, except i.c. group. The patterns of PK-resistant PrP in brains were basically identical among the five infection routes. Conclusion Typical TSE could be induced in hamsters by inoculating strain 263K in the five infection ways.The incubation periods in bioassays depend on infective dosage, administrating pathway and preparation of PrP^sc.The neuropathological changes and PrP^sc deposits seem to be related with regions and inoculating pathways. | JINZHANG LANCHEN BAO-YUNZHANG JUNHAN XIN-LIXIAO HAI-YANTIAN BIN-LINGLI CHENGAO JIAN-MEIGAO GUI-PINGMA CAI-MINXU YONGLIU XIAO-PINGDONG | 2004 | Biomedical and Environmental Sciences2004，17，1： | 7 |
| 4 | 文献快报(6):青少年数字媒体使用与后续注意缺陷多动障碍症状关联显示文摘现代数字媒体平台具有易获得性和强烈的刺激性,频繁使用现代数字媒体是否与青少年注意缺陷多动障碍(ADHD)症状存在关联尚未明确。一项来自南加卅I大学的纵向队列研究通过方便抽样的方法,对从洛杉矶10所高中招募的4100名15—16岁青少年开展为期24个月的随访调查(2014年9月至2016年12月,每6个月进行1次),基线调查共纳入3051名十年级学生。 | CHAELIN K R JUNHAN C H MATTHEW D S 陶心琢 陶舒曼 | 2018 | 中国学校卫生2018，39，8： | 6 |
| 5 | Theoretical modeling of steel reinforced ECC column under eccentric compressive loading显示文摘Fiber reinforced cementitious composites(ECC) are a class of advanced composites with strain hardening and multiple cracking behaviors. Substitution of concrete with ECC can significantly improve the seismic resistance and durability of the infrastructures. In this paper, it is proposed to use ECC as the matrix of frame columns for improving its load carrying capacity, ductility, and avoiding the brittleness of concrete. Based on the assumption of plane remaining plane and constitutive models of materials, theoretical models for calculating the load-carrying capacity of the steel reinforced ECC columns under small and large eccentric compression are proposed. With the parameters of the constitutive models from the existing experimental data, the relationship between ultimate axial load and moment capacities is also derived with the proposed models. To verify the validity of the proposed theoretical models, finite element analysis with the software of ATENA is conducted to simulate the mechanical behavior of the steel reinforced ECC columns under eccentric compressive loading. The calculation results from the theoretical models show good consistency with the simulated results, indicating that the proposed models are feasible and reliable for design. Finally, based on the theoretical models, the effect of the ultimate tensile strain and compressive strength of ECC, longitudinal reinforcement ratio on the load carrying capacity of the steel reinforced ECC column are comprehensively studied. | PAN JinLong GU Jie CHEN JunHan | 2015 | Science China(Technological Sciences)2015，58，5： | 4 |
| 6 | Efficient and Quick Inactivation of SARS Coronavirus and Other Microbes Exposed to the Surfaces of Some Metal Catalysts显示文摘Objective To study the two metal catalysts Ag/Al2O3 and Cu/Al2O3 that interdict the transmission pathway for SARS and other respiratory infectious diseases. Methods Two metal catalysts Ag/Al2O3 and Cu/Al2O3 were pressed into wafers. One hundred μL 106 TCID50/mL SARS-CoV, 100 μL 106 PFU/mL recombinant baculovirus expressing hamster’s prion protein (haPrP) protein and roughly 106 E. coli were slowly dropped onto the surfaces of the catalyst wafers and exposed for 5 and 20 min, respectively. After eluted from the surfaces of wafers, the infectivity of viruses and propagation of bacteria were measured. The expression of PrP protein was determined by Western blot. The morphological changes of bacteria were observed by electronic microscopy. Results After exposure to the catalysts surfaces for 5 and 20 min, the infectivity of SARS-CoV in Vero cells and baculovirus in Sf9 cells dropped down to a very low and undetectable level, and no colony was detected using bacteria culture method. The expression of haPrP protein reduced to 21.8% in the preparation of Sf9 cells infected with recombinant baculovirus exposed for 5 min and was undetectable exposed for 20 min. Bacterial membranes seemed to be cracked and the cytoplasm seemed to be effluent from cell bodies. Conclusion Exposures to the surfaces of Ag/Al2O3 and Cu/Al2O3 destroy the replication and propagation abilities of SARS-CoV, baculovirus and E. coli. Inactivation ability of metal catalysts needs to interact with air, utilizing oxygen molecules in air. Efficiently killing viruses and bacteria on the surfaces of the two metal catalysts has a promising potential for air-disinfection in hospitals, communities, and households. | JUNHAN LANCHEN SHU-MINDUAN QING-XIANGYANG MINYANG CHENGAO BAO-YUNZHANG HONGHE XIAO-PINGDONG | 2005 | Biomedical and Environmental Sciences2005，18，3： | 4 |
| 7 | A Verifier-based Password-authenticated Key Exchange Protocol via Elliptic Curves显示文摘 | Yang Junhan Cao Tianjie | | 0，，02： | 1 |
| 8 | Study of the currentstatus and factors that influence indoor air pollution in 138 housesin the urban area in Xi,an显示文摘 | LIU Junhan GUO Yuraing PAN Xiaochuan | 2008 | Environmental Challenges in thePacific Basin2008，1140，： | 1 |
| 9 | High-voltage insulation organic-lnorganic nanocomposites by plasma polymerization 显示文摘 | Wei Yan Zhao Junhan Phung B Toan | 2014 | Materials2014，7，1： | 1 |
| 10 | Processing and structure relationships in electrospining of ceramic fiber systems显示文摘 | Sigmund Wolfgang Yuh Junhan | 2006 | J Am Ceram Soc2006，89，2： | 1 |
| 11 | Macular pigments lutein and zeaxanthin as blue light tilters studied inliposomes显示文摘 | Junhans A Sies H Stahl W | 2001 | Archives of Biochemistrty and Biophysics2001，391，2： | 1 |
| 12 | Mychonastes afer HSO - 3 - 1 as a potential new source of biodiesel 显示文摘 | YUAN Cheng LIU Junhan FAN Yong | 2011 | Biotechnol Biofuels2011，4，1： | 1 |
| 13 | Use of ultrasonic array method for positioning multiple partial dis- charge sources in transformer oil显示文摘 | XIE Qing TAO Junhan WANG Yongqiang | 2014 | Review of Scientific Instruments2014，85，08： | 1 |
| 14 | Modelling the thresholds of nitrogen/phosphorus concentration and hydraulic retention time for bloom control in reclaimed water landscape显示文摘The risks posed by algal blooms caused by nitrogen and phosphorus in reclaimed water used in urban water landscapes need to be carefully controlled.In this study,the combined effects of the nitrogen and phosphorus concentrations and the light intensity and temperature on the specific growth rates of algae were determined using Monod,Steele,and Arrhenius models,then an integrated algal growth model was developed.The algae biomass,nitrogen concentration,and phosphorus concentration mass balance equations were used to establish a new control model describing the nitrogen and phosphorus concentration and hydraulic retention time thresholds for algal blooms.The model parameters were determined by fitting the models to data acquired experimentally.Finally,the control model and numerical simulations for six typical algae and mixed algae under standard conditions were used to determine nitrogen/phosphorus concentration and hydraulic retention time thresholds for landscape water to which reclaimed water is supplied(i.e.,for a reclaimed water landscape). | Keying Song Shufeng Zhu Yun Lu Guohua Dao Yinhu Wu Zhuo Chen Shengnan Wang Junhan Liu Wenguang Zhou Hong-Ying Hu | 2022 | Frontiers of Environmental Science & Engineering2022，16，10： | 1 |
| 15 | Provably Secure Three-party Password Authenticated Key Exchange Protocol in the Standard Model显示文摘 | Yang Junhan Cao Tianjie | 2012 | Journal of Systems and Software2012，85，2： | 1 |
| 16 | Synthesis of barium titanate (BaTiO 3 ) nanofibers via electrospinning显示文摘 | Junhan Yuh Juan C. Nino Wolfgang M. Sigmund | 2005 | Materials Letters2005，，： | 1 |
| 17 | Lipidomic analyses reveal enhanced lipolysis in planthoppers feeding on resistant host plants显示文摘The brown planthopper(BPH)(Nilaparvata lugens St?l)is a highly destructive pest that seriously damages rice(Oryza sativa L.)and causes severe yield losses.To better understand the physiological and metabolic mechanisms through which BPHs respond to resistant rice,we combined mass-spectrometry-based lipidomics with transcriptomic analysis and gene knockdown techniques to compare the lipidomes of BPHs feeding on either of the two resistant(NIL-Bph6 and NIL-Bph9)plants or a wild-type,BPH susceptible(9311)plant.Insects that were fed on resistant rice transformed triglyceride(TG)to phosphatidylcholine(PC)and digalactosyldiacylglycerol(DGDG),with these lipid classes showing significant alterations in fatty acid composition.Moreover,the insects that were fed on resistant rice were characterized by prominent expression changes in genes involved in lipid metabolism processes.Knockdown of the NlBmm gene,which encodes a lipase that regulates the mobilization of lipid reserves,significantly increased TG content and feeding performance of BPHs on resistant plants relative to dsGFP-injected BPHs.Our study provides the first detailed description of lipid changes in BPHs fed on resistant and susceptible rice genotypes.Results from BPHs fed on resistant rice plants reveal that these insects can accelerate TG mobilization to provide energy for cell proliferation,body maintenance,growth and oviposition. | Xiaohong Zheng Yeyun Xin Yaxin Peng Junhan Shan Ning Zhang Di Wu Jianping Guo Jin Huang Wei Guan Shaojie Shi Cong Zhou Rongzhi Chen Bo Du Lili Zhu Fang Yang Xiqin Fu Longping Yuan Guangcun He | 2021 | Science China(Life Sciences)2021，64，9： | 1 |
| 18 | DOCK2 regulates antifungal immunity by regulating RAC GTPase activity显示文摘Fungal infections cause~1.5 million deaths each year worldwide,and the mortality rate of disseminated candidiasis currently exceeds that of breast cancer and malaria.The major reasons for the high mortality of candidiasis are the limited number of antifungal drugs and the emergence of drug-resistant species.Therefore,a better understanding of antifungal host defense mechanisms is crucial for the development of effective preventive and therapeutic strategies.Here,we report that DOCK2(dedicator of cytokinesis 2)promotes indispensable antifungal innate immune signaling and proinflammatory gene expression in macrophages.DOCK2-deficient macrophages exhibit decreased RAC GTPase(Rac family small GTPase)activation and ROS(reactive oxygen species)production,which in turn attenuates the killing of intracellular fungi and the activation of downstream signaling pathways.Mechanistically,after fungal stimulation,activated SYK(spleen-associated tyrosine kinase)phosphorylates DOCK2 at tyrosine 985 and 1405,which promotes the recruitment and activation of RAC GTPases and then increases ROS production and downstream signaling activation.Importantly,nanoparticle-mediated delivery of in vitro transcribed(IVT)Rac1 mRNA promotes the activity of Rac1 and helps to eliminate fungal infection in vivo.Taken together,this study not only identifies a critical role of DOCK2 in antifungal immunity via regulation of RAC GTPase activity but also provides proof of concept for the treatment of invasive fungal infections by using IVT mRNA. | Xiaojian Ma Xi Tan Bingbing Yu Wanwei Sun Heping Wang Huijun Hu Yanyun Du Ruirui He Ru Gao Qianwen Peng Zhihui Cui Ting Pan Xiong Feng Junhan Wang Chengqi Xu Bin Zhu Wei Liu Chenhui Wang | 2022 | Cellular & Molecular Immunology2022，19，5： | 1 |
| 19 | Influence of Mo Content on Microstructure and Mechanical Properties of High Strength Pipeline Steel显示文摘 | Kong Junhan Zhen Lin | 2003 | Material and Design2003，，25： | 1 |
| 20 | Boosting fire safety and mechanical performance of thermoplastic polyurethane by the face-to-face two-dimensional phosphorene/MXene architecture显示文摘Black phosphorus(BP), as one of the most promising fillers for flame retarding polymer, has been seriously limited in practical application, due to the agglomeration and poor structural stability challenges.Here, the BP was modified by MXene and polydopamine(PDA) via ultrasonication and dopamine modification strategy to improve the structural stability and dispersibility in the matrix. Then, the obtained(BP-MXene@PDA) nanohybrid was employed to promote the mechanical performance, thermal stability,and flame retardancy of thermoplastic polyurethane elastomer(TPU). The resultant TPU composite containing 2 wt.% of BP1-MXene2@PDA showed a 19.2% improvement in the tensile strength and a 13.8%increase in the elongation at break compared to those of the pure TPU. The thermogravimetric analysis suggested that BP-MXene@PDA clearly enhances the thermal stability of TPU composites. Furthermore,the introduction of the BP-MXene@PDA nanohybrids could considerably improve the flame retardancy of TPU composite, i.e., 64.2% and 27.3% decrease in peak heat release rate and total heat release, respectively. The flame-retardant mechanisms of TPU/BP-MXene@PDA in the gas phase and condensed phase were investigated systematically. This work provides a novel strategy to simultaneously enhance the fire safety and mechanical properties of TPU, thus expanding its industrial applications. | Yong Luo Yuhui Xie Wei Geng Junhan Chu Hua Wu Delong Xie Xinxin Sheng Yi Mei | 2022 | Journal of Materials Science & Technology2022，，34： | 1 |
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