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| 1 | HIF-1α-induced expression of m6A reader YTHDF1 drives hypoxia-induced autophagy and malignancy of hepatocellular carcinoma by promoting ATG2A and ATG14 translation显示文摘N6-methyladenosine(m6A),and its reader protein YTHDF1,play a pivotal role in human tumorigenesis by affecting nearly everystage of RNA metabolism.Autophagy activation is one of the ways by which cancer cells survive hypoxia.However,the possibleinvolvement of m6A modification of mRNA in hypoxia-induced autophagy was unexplored in human hepatocellular carcinoma(HCO).In this study,specific variations in YTHDF1 expression were detected in YTHDF1-overexpressing,knockout,and-knockdownHCC cells,HCC organoids,and HCC patient-derived xenograft(PDX)murine models.YTHDF1 expression and hypoxia inducedautophagy were significantly correlated in vitro;signifhcant overexpression of YTHDF1 in HCC tissues was associated with poorprognosis,Multivariate cox regression analysis identihed YTHDF1 expression as an independent prognostic factor in patients withHCC.Multiple HC models conhrmed that YTHDF1 deficiency inhibited HCC autophagy,growth,and metastasis.Luciferase reporterassays and chromatin immunoprecipitation demonstrated that HlIF-1a regulated YTHDF1 transcription by directly binding to itspromoter region under hypoxia.The results of methylated RNA immunoprecipitation sequencing,proteomics,and polysomeprofling indicated that YTHDF1 contibuted to the translation of autophagy-related genes ATG2A and ATG14 by binding to m6A-modifhed ATG2A and ATG14 mRNA,thus facilitating autophagy and autophagy-related malignancy of HCC.Taken together,HlE-1d-induced YTHDF1 expression was associated with hypoxia-induced autophagy and autophagy-related HCC progression via promoting translation of autophagy-related genes ATG2A and ATG14 in a m6A-dependent manner.Our fndings suggest thatYTHDF1 is a potential prognostic biomarker and therapeutic target for patients with HCC. | Qing Li Yong Ni Liren Zhang Runqiu Jiang Jing Xu Hong Yang Yuanchang Hu Jiannan Qiu Liyong Pu Jinhai Tang Xuehao Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 24 |
| 2 | Omics-based integrated analysis identified ATRX as a biomarker associated with glioma diagnosis and prognosis显示文摘Objective:ATRX is a multifunctional protein that is tightly regulated by and implicated in transcriptional regulation and chromatin remodeling.Numerous studies have shown that genetic alterations in ATRX play a significant role in gliomas.This study aims to further determine the relationship between ATRX and glioma prognosis and identify possible mechanisms for exploring the biological significance of ATRX using large data sets.Methods:We used The Cancer Genome Atlas(TCGA)database and 130 immunohistochemical results to confirm the difference in ATRX mutations in high-and low-grade gliomas.An online analysis of the TCGA glioma datasets using the cBioPortal platform was performed to study the relationship between ATRX mutations and IDH1,TP53,CDKN2 A and CDKN2 B mutations in the corresponding TCGA glioma dataset.In combination with clinical pathology data,the biological significance of the relationships were analyzed.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses and annotations of all adjacent genes in the network were performedin the Database for Annotation,Visualization and Integrated Discovery(DAVID)and R language.A protein-protein interaction(PPI)network was constructed,and the interactions of all adjacent nodes were analyzed by the String database and using Cytoscape software.Results:In the selected TCGA glioma datasets,a total of 2,228 patients were queried,21%of whom had ATRX alterations,which co-occurred frequently with TP53 and IDH1 mutations.ATRX alterations are associated with multiple critical molecular events,which results in a significantly improved overall survival(OS)rate.In low-grade gliomas,ATRX mutations are significantly associated with multiple important molecular events,such as ZNF274 and FDXR at mRNA and protein levels.A functional cluster analysis revealed that these genes played a role in chromatin binding and P53,and a link was observed between ATRX and IDH1 and TP53 in the interaction network.ATRX and TP53 are important nodes in the network and have potential links with the blood oxygen imbalance.Conclusions:ATRX mutations have clinical implications for the molecular diagnosis of gliomas and can provide diagnostic and prognostic information for gliomas.ATRX is expected to serve as a new therapeutic target. | Yingbin Xie Yanli Tan Chao Yang Xuehao Zhang Can Xu Xiaoxia Qiao Jianglong Xu Shaohui Tian Chuan Fang Chunsheng Kang | 2019 | Cancer Biology & Medicine2019，16，4： | 5 |
| 3 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 4 | Laboratory core flooding experimental systems for CO_2 geosequestration: An updated review over the past decade显示文摘Carbon dioxide(CO_2) geosequestration in deep saline aquifers has been currently deemed as a preferable and practicable mitigation means for reducing anthropogenic greenhouse gases(GHGs) emissions to the atmosphere, as deep saline aquifers can offer the greatest potential from a capacity point of view. Hence,research on core-scale CO_2/brine multiphase migration processes is of great significance for precisely estimating storage efficiency, ensuring storage security, and predicting the long-term effects of the sequestered CO_2 in subsurface saline aquifers. This review article initially presents a brief description of the essential aspects of CO_2 subsurface transport and geological trapping mechanisms, and then outlines the state-of-the-art laboratory core flooding experimental apparatus that has been adopted for simulating CO_2 injection and migration processes in the literature over the past decade. Finally, a summary of the characteristics, components and applications of publicly reported core flooding equipment as well as major research gaps and areas in need of further study are given in relevance to laboratory-scale core flooding experiments in CO_2 geosequestration under reservoir conditions. | Yankun Sun Qi Li Duoxing Yang Xuehao Liu | 2016 | Journal of Rock Mechanics and Geotechnical Engineering2016，8，1： | 3 |
| 5 | Microstructure and compressive/tensile characteristic of large size Zr-based bulk metallic glass prepared by laser solid forming显示文摘The large size, crack-free Zr_(55)Cu_(30)Al_(10)Ni_(5) bulk metallic glass(BMGs) with the diameter of 54 mm and the height of 15 mm was built by laser solid forming additive manufacturing technology, whose size is larger than the critical diameter by casting. The microstructure, tensile and compressive deformation behaviors and fracture morphology of laser solid formed Zr_(55)Cu_(30)Al_(10)Ni_5 BMGs were investigated. It is found that the crystallization mainly occurs in the heat-affected zones of deposition layers, which consist of Al_5Ni_3Zr_2, NiZr_2, ZrCu, CuZr_2 phases. The content of amorphous phase in the deposit is about 63%.Under the compressive loading, the deposit presents no plasticity before fracture occurs. The fracture process is mainly controlled by the shear stress and the compressive shear fracture angles of about39?. The compressive strength reaches 1452 MPa, which is equivalent to that of as-Cast Zr_(55)Cu_(30)Al_(10)Ni_5 BMGs, and there exist vein-like patterns, river-like patterns and smooth regions at the compressive fractography. Under the tensile loading, the deposit presents the brittle fracture pattern without plastic deformation. The fracture process exhibits normal fracture model, and the tensile shear fracture angle of about 90?. The tensile strength is only about 609 MPa, and the tensile fractography mainly consists of micro-scaled cores and vein-like patterns, dimple-like patterns, chocolate-like patterns and smooth regions. The results further verified the feasibility and large potential of laser additive manufacturing on fabrication and industrial application of large-scale BMGs parts. | Xin Lin Yuanyuan Zhang Gaolin Yang Xuehao Gao Qiao Hu Jun Yu Lei Wei Weidong Huang | 2019 | Journal of Materials Science & Technology2019，35，2： | 1 |
| 6 | Cucumber glossy fruit 1 (CsGLF1) encodes the zinc finger protein 6 that regulates fruit glossiness by enhancing cuticular wax biosynthesis显示文摘Cucumber glossiness is an important visual quality trait that affects consumer choice.Accumulating evidence suggests that glossy trait is associated with cuticular wax accumulation.However,the molecular genetic mechanism controlling cucumber glossiness remains largely unknown.Here,we report the map-based cloning and functional characterization of CsGLF1,a locus that determines the glossy trait in cucumber.CsGLF1 encodes a homolog of the Cys_(2)His_(2)-like fold group(C2H2)-type zinc finger protein 6(ZFP6)and its deletion leads to glossier pericarp and decreased cuticular wax accumulation.Consistently,transcriptomic analysis demonstrated that a group of wax biosynthetic genes were downregulated when CsZFP6 was absent.Further,transient expression assay revealed that CsZFP6 acted as a transcription activator of cuticular wax biosynthetic genes.Taken together,our findings demonstrated a novel regulator of fruit glossiness,which will provide new insights into regulatory mechanism of fruit glossiness in cucumber. | Yan Yang Congxi Cai Yipeng Wang Yanran Wang Haolun Ju Xuehao Chen | 2023 | Horticulture Research2023，10，1： | 1 |
| 7 | Production of lipase by repeated batch fermentation with immobilized Rhizop us arrhizus显示文摘 | Yang Xuehao Wang Bingwu Cui Fengnan | 2005 | Process Biochemistry2005，40，： | 1 |
| 8 | Production of lipase by repeated batch fermentation with immobilized Rhizopus arrhizus显示文摘 | Yang Xuehao Wang Bingwu Cui Fengnan | 2005 | Process Biochemistry2005，40，： | 1 |
| 9 | Chiral AuCu heterostructures with site-specific geometric control and tailored plasmonic chirality显示文摘Rational design and construction of chiral-achiral hybrid structures are of great importance to realize the multifunctional complex chiral structures toward emerging technological applications. However, significant challenges remain due to the lack of fine control over the heterostructure. Here, we have developed a general bottom-up synthetic strategy for the site-selective growth of Cu nanodomains on intrinsically chiral Au nanocrystals. Chiral AuCu heterostructures with three distinct architectures were achieved by controlling the overgrowth of Cu nanodomains in a site-specific manner. The geometry-dependent plasmonic chirality of the heterostructures was demonstrated experimentally by circular dichroism spectroscopy and theoretically through finite-difference time-domain simulations. The site-specific geometric control of chiral AuCu heterostructures was also extended to employ anisotropic chiral Au nanoplates and nanorods as the building blocks. By virtue of the galvanic replacement reactions between metal ions and Cu atoms, chiral heterostructures with increasing architectural complexity and compositional diversity can be further achieved. The current work not only opens up a promising strategy to synthesize complex chiral hybrid nanostructures but also provides an important knowledge framework that guides the rational design of multifunctional chiral hybrid nanostructures toward chiroptical applications. | Guizeng Yang Lichao Sun Yunlong Tao Qingqing Cheng Xuehao Sun Chuang Liu Qingfeng Zhang | 2023 | Science China Chemistry2023，66，11： | 0 |
| 10 | In-situ monitored chemical bath deposition of planar NiO x layer for inverted perovskite solar cell with enhanced efficiency显示文摘As a convenient,low-cost and up-scalable solution route,chemical bath deposition(CBD)has exhibited impressive advantages in fabricating electron transporting materials like SnO_(2),achieving record efficien-cies for regular n-i-p perovskite solar cells(PSCs).However,for the hysteresis-free and potentially more stable inverted p-i-n PSCs,CBD processing is rarely studied to improve the device performance.In this work,we first present a CBD planar NiO x film as the efficient hole transport layer for the inverted per-ovskite solar cells(IPSCs).The morphologies and semiconducting properties of the NiO x film can be ad-justed by varying the concentration of[Ni(H 2 O)x(NH 3)6-x]2+cation via in-situ monitoring of the CBD re-action process.The characterizations of ultraviolet photoelectron spectroscopy,transient absorption spec-troscopy,time-resolved photoluminescence suggest that the CBD planar NiO x film possesses enhanced conductivity and aligned energy band levels with perovskite,which benefits for the charge transport in the IPSCs.The devices based on planar NiO x at 50°C and low nickel precursor concentration achieved an enhanced efficiency from 16.14%to 18.17%.This work established an efficient CBD route to fabricate planar NiO x film for PSCs and paved the way for high performance PSCs with CBD-prepared hole transporting materials. | Liqi Li Wenjian Shen Chenquan Yang Yuxi Dou Xuehao Zhu Yao Dong Juan Zhao Junyan Xiao Fuzhi Huang Yi-Bing Cheng Jie Zhong | 2023 | Journal of Materials Science & Technology2023，，2： | 0 |
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